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Gelrite

Gelrite is a bacterial polysaccharide that forms a thermoreversible gel when dissolved in water.
It is commonly used as a gelling agent in plant tissue culture media, providing a semi-solid support for plant growth and development.
Gelrite exhibits excellent thermal stability and transparency, making it a popular alternative to tradtional agar.
Researchers can leverage AI-powered tools like PubCompare.ai to efficiently locate and compare the best Gelrite protocols from published literature, preprints, and patents, optimizing their experimental procedures and driving their discoveries forward.

Most cited protocols related to «Gelrite»

Cultivation and Selection of Sulfolobus Mutants

Cited 19 times

Sulfolobus acidocaldarius DSM 639, MW001, and all constructed deletion mutants were aerobically grown in Brock media (Brock et al., 1972 (link)) with a pH of 3 at 76°C. The media were supplemented with 0.1% (w/v) tryptone or with 0.1% (w/v) N-Z-Amine and 0.2% dextrine. The growth of the cells was monitored by measurement of the optical density at 600 nm.
For pouring Sulfolobus plates a two times concentrated Brock media was supplemented with 6 mM CaCl₂ and 20 mM MgCl₂. For first selection plates 0.2% NZ-Amine (Fluka) and 0.4% dextrin, for second selection plates 0.2% tryptone, 0.4% dextrin, 200 μg/ml 5-FOA, and 20 μg/ml uracil was added to the two times concentrated solution and prewarmed to 75°C. This solution was mixed with an equal volume of fresh boiling 1.4% Gelrite solution (Carl Roth, Karlsruhe, Germany) and poured in 40 ml portions into petri dishes (150 × 20 mm, Sarstedt, Nümbrecht, Germany).

Wagner M., van Wolferen M., Wagner A., Lassak K., Meyer B.H., Reimann J, & Albers S.V. (2012). Versatile Genetic Tool Box for the Crenarchaeote Sulfolobus acidocaldarius. Frontiers in Microbiology, 3, 214.

Publication 2012

Amines Deletion Mutation Dextrin Gelrite Hyperostosis, Diffuse Idiopathic Skeletal Magnesium Chloride Sulfolobus Sulfolobus acidocaldarius Uracil

Screening Polysaccharide-Degrading Enzymes

Cited 6 times

1% agar (catalog number A1296, Sigma-Aldrich Corp., St. Louis, MO, USA) containing 0.2% carboxymethylcellulose (catalog number C4888, Sigma-Aldrich Corp., St. Louis, MO, USA) was poured into petri dishes or into the wells of a 96-well plate, respectively. Stock solutions of polymer-degrading enzymes were dissolved in 1× Tris-buffered saline (TBS) buffer or deionized water, following the suppliers’ instructions for reconstitution of the enzyme. Spot plating was performed with 5 μL of commercial cellulase from Aspergillus niger (catalog number C1184, Sigma-Aldrich Corp., St. Louis, MO, USA) at different concentrations (0.1 and 5 μg/μL). Agarase (1 μg/μL) (catalog number EO0461, Fermentas, Burlington, ON, Canada), amylase (1 μg/μL) (catalog number 10065, Sigma-Aldrich Corp., St. Louis, MO, USA) and proteinase K (1 μg/μL) (catalog number EO0491, Fermentas, Burlington, ON, Canada) were used as control enzymes. In some cases, agar was replaced by 1% agarose or 0.75% Gelrite with or without 0.2% CMC, respectively. All plates were incubated at 27 °C for 12–16 h after which hydrolysis zones were visualized by flooding of the plates/wells with Gram’s iodine (2 g potassium iodide and 1 g iodine in 300 mL water) for 5 min followed by a rinses with deionized water. For detection with Congo Red, the plates were flooded with 0.1% Congo Red for 15–20 min and then rinsed with 1 M NaCl. Plates where CMC was omitted were used as non-substrate controls in all experiments.

Johnsen H.R, & Krause K. (2014). Cellulase Activity Screening Using Pure Carboxymethylcellulose: Application to Soluble Cellulolytic Samples and to Plant Tissue Prints. International Journal of Molecular Sciences, 15(1), 830-838.

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Publication 2014

Agar agarase Amylase Aspergillus niger Carboxymethylcellulose Cellulase Endopeptidase K Enzymes Gelrite Hydrolysis Hyperostosis, Diffuse Idiopathic Skeletal Iodine Polymers Potassium Iodide Saline Solution Sepharose Sodium Chloride

Growth Conditions for Arabidopsis and Brachypodium

Cited 5 times

Arabidopsis seeds were planted in wet LC-1 potting soil mix (Sun Gro
Horticulture, Agawam, MA, USA) and plants were grown in a growth chamber with
controlled conditions: 16-h light/ 8-h dark at 21°C, with relative humidity of
65% and light intensity of 160 μmol s^-1 m^-2.
For the extensin quantification experiments, Arabidopsis seedlings
were grown on plates in the same growth chamber as above. Seeds were sterilized with
sequential treatments of 70% ethanol and 0.5% bleach, washed multiple times with
sterile water and planted on -strength Murashige and Skoog medium (Murashige and Skoog, 1962 (link)) with 2% sucrose and
0.3% Gelrite (Research Products International, Mt. Prospect, IL, USA).
Brachypodium plants were grown in the same conditions as
Arabidopsis. After a vernalization period of 14 days in the dark
at 4°C to maximize germination rate, pots were transferred to a growth chamber
maintained at 21°C, 65% relative humidity, and 160 μmol s^-1m^-2 light intensity.

Reem N.T., Pogorelko G., Lionetti V., Chambers L., Held M.A., Bellincampi D, & Zabotina O.A. (2016). Decreased Polysaccharide Feruloylation Compromises Plant Cell Wall Integrity and Increases Susceptibility to Necrotrophic Fungal Pathogens. Frontiers in Plant Science, 7, 630.

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Publication 2016

Arabidopsis Brachypodium Ethanol Gelrite Germination Growth Disorders Humidity Light Marijuana Abuse Plant Embryos Plants Sucrose

Isolation and Purification of Methanotrophic Bacteria

Cited 5 times

The primary enrichment cultures were transferred five times in fresh LMM medium. Then, serial dilutions were made, and 100 μL of dilutions (10⁻⁵ to 10⁻⁸) were spread onto plates containing LMM medium solidified with gelrite (20 g·L⁻¹; Gelzan^TM CM, Sigma-Aldrich Corporation, St. Louis, MO, USA). The gelrite plates were incubated for two to four weeks at room temperature (22 °C) in jars filled with methane gas and air. Individual colonies were re-streaked onto fresh plates and re-incubated under the same conditions for two to three weeks, and this process was repeated twice. One strain from each of the environments, wet rice field soil, methane seep and warm spring sediments, was isolated and designated as strains BRS-K6, GFS-K6 and AK-K6, respectively. LMM medium was used for routine cultivation at 25 °C with methane and air in the head space. The purity of cultures was assessed by phase-contrast and transmission electron microscopy (TEM, at 60 KV, Jeol JEM-1230, Tokyo, Japan), the observation of single colony growth on gelrite plates in atmosphere without methane, heterotrophic contamination tests in Luria-Bertani broth in dilution (1 to 5% v/v) and on R2A (Reasoner’s 2A agar) plates [29 (link)]. The absence of growth on these media together with microscopy observation confirmed the purity of the cultures.

Islam T., Larsen Ø., Torsvik V., Øvreås L., Panosyan H., Murrell J.C., Birkeland N.K, & Bodrossy L. (2015). Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats. Microorganisms, 3(3), 484-499.

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Publication 2015

Agar Atmosphere Gelrite Head Heterotrophy Methane Microscopy Microscopy, Phase-Contrast Oryza sativa Strains Technique, Dilution Transmission Electron Microscopy

Isolation of Chemolithotrophic Iron-Oxidizing Bacteria

Cited 4 times

The strains K2D and TBK1r were isolated from iron-hydroxide deposits obtained at a depth of 1,734 m from the Valu Fa Ridge in the Pacific Ocean14. In brief, strain K2D was obtained from an inoculum in M30 medium53 with 70% aged seawater and 200 mg l⁻¹ ampicillin for enrichment culturing. The samples were added at ratios of 1:10 and 1:100, and incubated at room temperature. The enrichments were examined through light microscopy after one week. Following this, 1:100 enrichments were plated onto M30 medium gelrite plates with 70% aged seawater, 200 mg l⁻¹ streptomycin and 200 mg l⁻¹ ampicillin. Small colorless colonies were observed after two weeks and the strain was purified by continuous re-streaking on M30 gelrite plates. The TBK1r strain was obtained from an iron-hydroxide dilution series in PBS (tenfold dilution series up to 1:10⁴). A 100-μl volume of these dilutions was then directly plated onto seawater–peptone–yeast extract–gelrite plates with 200 mg l⁻¹ streptomycin and 200 mg l⁻¹ ampicillin. Pink colonies appeared after two weeks and these were examined using light microscopy and streaked on new plates; the colonies displaying budding growth or rosette formation were re-streaked. Strain SV_7m_r was isolated from a meromictic lake in Bergen, Norway. Liquid M30 medium was inoculated with meromictic lake water from a depth of 7 m (at a dilution of 1:10 and 1:100) and incubated at room temperature. After an incubation period of three weeks, 10 μl of the enrichment was plated onto solid M13 gelrite plates with 200 mg l⁻¹ ampicillin. Small red colonies were observed after three weeks and the strain was purified by continuous re-streaking on M13 gelrite plates.

Wiegand S., Jogler M., Boedeker C., Pinto D., Vollmers J., Rivas-Marín E., Kohn T., Peeters S.H., Heuer A., Rast P., Oberbeckmann S., Bunk B., Jeske O., Meyerdierks A., Storesund J.E., Kallscheuer N., Lücker S., Lage O.M., Pohl T., Merkel B.J., Hornburger P., Müller R.W., Brümmer F., Labrenz M., Spormann A.M., Op den Camp H.J., Overmann J., Amann R., Jetten M.S., Mascher T., Medema M.H., Devos D.P., Kaster A.K., Øvreås L., Rohde M., Galperin M.Y, & Jogler C. (2019). Cultivation and functional characterization of 79 planctomycetes uncovers their unique biology. Nature microbiology, 5(1), 126-140.

Publication 2019

Ampicillin Gelrite hydroxide ion Immunocytoadherence Iron Light Microscopy Peptones Strains Streptomycin Technique, Dilution Yeast, Dried

Most recents protocols related to «Gelrite»

Anaerobic Cultivation of Thermococcus kodakarensis

Thermoccocus kodakarensis was isolated from Kodakara Island, Kagoshima, Japan (Morikawa et al., 1994 (link); Atomi et al., 2004 (link)). T. kodakarensis KU216 (Sato et al., 2003 (link), 2005 (link)) and derivative strains were cultivated under strictly anaerobic conditions at 85°C in nutrient-rich medium (ASW-YT-m1-S⁰ or ASW-YT-m1-pyruvate) or synthetic medium (ASW-AA-m1-S⁰). ASW-YT-m1-S⁰, ASW-YT-m1-pyruvate, and ASW-AA-m1-S⁰ are modified versions of ASW-YT-S⁰, ASW-YT-pyruvate, and ASW-AA-S⁰ media, respectively. ASW-YT-S⁰ was composed of 0.8 × artificial seawater (ASW) (Robb and Place, 1995 ), 5 g L⁻¹ yeast extract, 5 g L⁻¹ tryptone, and 2 g L⁻¹ elemental sulfur. In ASW-YT-m1-S⁰, 20 μM KI, 20 μM H₃BO₃, 10 μM NiCl₂, and 10 μM Na₂WO₄ were supplemented. In ASW-YT-m1-pyruvate medium, elemental sulfur was replaced with 5 g L⁻¹ sodium pyruvate. ASW-AA-S⁰ was composed of 0.8 × ASW, a mixture of 20 amino acids, modified Wolfe’s trace minerals and a mixture of vitamins (Sato et al., 2003 (link)). In ASW-AA-m1-S⁰, 20 μM KI, 20 μM H₃BO₃, 10 μM NiCl₂, and 10 μM Na₂WO₄ were supplemented, and the concentrations of l-arginine hydrochloride and l-valine were increased (from 125 mg L⁻¹ to 250 mg L⁻¹ and from 50 mg L⁻¹ to 200 mg L⁻¹, respectively). When cells without a pyrF gene were grown, 10 μg mL⁻¹ uracil was added to make ASW-AA-m1-S⁰(+Ura). To remove oxygen in the medium, 5% (w/v) Na₂S solution was added until the medium became colorless. Resazurine (0.5 mg L⁻¹) was also added to all media as an oxygen indicator. For solid medium used to isolate transformants, 10 g L⁻¹ gelrite, 7.5 g L⁻¹ 5-fluoroorotic acid (5-FOA), 10 μg mL⁻¹ uracil, 4.5 mL of 1 M NaOH and 0.2% (v/v) polysulfide solution (10 g Na₂S 9H₂O and 3 g sulfur flowers in 15 mL H₂O) rather than elemental sulfur was supplemented to ASW-AA-m1 medium. Escherichia coli DH5α (Takara Bio, Kusatsu, Japan) and BL21-Codonplus(DE3)-RIL strains (Agilent Technologies, Santa Clara, CA) were cultivated at 37°C in Lysogeny broth (LB) medium supplemented with ampicillin (100 mg L⁻¹). E. coli DH5α was used for recombinant plasmid construction and E. coli BL21-Codonplus (DE3)-RIL was used for heterologous gene expression. Chemicals were purchased from Wako Pure Chemicals (Osaka, Japan) or Nacalai Tesque (Kyoto, Japan) unless mentioned otherwise.

Su Y., Michimori Y, & Atomi H. (2023). Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis. Frontiers in Microbiology, 14, 1126218.

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Publication 2023

5-fluoroorotic acid Amino Acids Ampicillin Arginine Hydrochloride Cells Escherichia coli Flowers Gelrite Gene Expression Genes Lysogeny Nutrients Oxygen Plasmids polysulfide Pyruvate Sodium sodium sulfide Strains Sulfur Trace Minerals Uracil Valine Vitamin A Yeast, Dried

Cultivation and Genetic Manipulation of Saccharolobus islandicus

Saccharolobus islandicus REY15A was grown aerobically at 75°C in STV medium containing mineral salt, 0.2% (w/v) sucrose (S), 0.2% (w/v) tryptone (T), and a mixed vitamin solution (V). Saccharolobus islandicus REY15A(E233S)(ΔpyrEFΔlacS), hereafter E233S, was grown in STVU (STV supplemented with 0.01% (w/v) uracil) medium. The medium was adjusted to pH 3.3 with sulfuric acid, as described previously (29 (link)). SCV medium containing 0.2% (w/v) casamino acid (C) was used for screening and cultivating uracil prototrophic transformants. ATV medium containing 0.2% (w/v) D-arabinose (A) was used for protein expression. Culture plates were prepared using gelrite (0.8% [w/v]) by mixing 2 × STV and an equal volume of 1.6% gelrite. The strains constructed and used in this study are listed in the Supplementary information (Supplementary Table S1).

Yang Y., Liu J., Fu X., Zhou F., Zhang S., Zhang X., Huang Q., Krupovic M., She Q., Ni J, & Shen Y. (2023). A novel RHH family transcription factor aCcr1 and its viral homologs dictate cell cycle progression in archaea. Nucleic Acids Research, 51(4), 1707-1723.

Publication 2023

Acids Arabinose Gelrite Minerals Proteins Salts Strains Sucrose sulfuric acid Uracil Vitamins

Optimized Gynostemma pentaphyllum Cell Culture

The GP plant was collected from Sadong-ri, Ulleung-eup, Ulleung-gun, Gyeongsangbuk-do in Korea on July 1, 2020 by Dr. Sang Hyun Moh. The rbcL amplicon sequence from the collected GP plant shared 100% sequence identity to that of Gynostemma pentaphyllum (Thunb.) Makino (Registration number: NIBRGR0000654599) deposited in the National Institute of Biological Resources (https://species.nibr.go.kr (accessed on 3 July 2022)).
The leaves were soaked in 70% ethanol for 30 s, followed by washing with distilled water. The leaves were shaken in 0.3% sodium hypochlorite (Waco, Osaka, Japan) for 20 min and washed again with distilled water. The leaves were cut into small pieces (0.5 to 1 cm) under aseptic conditions. The early stages of the plant cells were cultured in Murashige and Skoog (MS) medium (M0222, Duchefa, Haarlem, The Netherlands) [28 (link)] with plant growth regulators, auxin and cytokinin, in the dark at 25 ± 2 °C. The first induced plant cell was propagated in the same petri dish for 2–3 weeks. From the eighth week, the optimal combination ratio of dichlorophenoxyacetic acid (2,4-D; D0911, Duchefa) was chosen based on the color, shape, and degree of differentiation of the plant cells. The selected plant cell line or callus was propagated in the optimal medium containing 4.4 g/L of MS, 30 g/L sucrose (S0809, Duchefa), 1 mg/L 2,4-D, 2.3 g/L gelrite (G1101, Duchefa) at pH 5.8 in a petri dish. Thereafter, the callus was cultured in a bioreactor at the Plant Cell Research Institute of BIO-FD&C Co., Ltd., Incheon, Republic of Korea.
Freshly prepared plant cell cultures of GP were filtered to harvest the plant cells from the culture media. Thereafter, 100 g each of the cells were transferred to three bioreactors (total volume three liters), with each bioreactor containing 2 L of fresh MS (4.4 g/L, pH 5.8) basal medium supplemented with 30 g/L of sucrose and 1 mg/L 2,4-D.

Cho W.K., Paek S.H., Kim S.Y., Jang S.J., Lee S., Choi H., Jo Y., Lee J.H, & Moh S.H. (2023). Comparative Transcriptome Profiles of Human HaCaT Cells in Response to Gynostemma pentaphyllum Extracts Obtained Using Three Independent Methods by RNA Sequencing. Life, 13(2), 423.

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Publication 2023

Acids Asepsis Auxins Biopharmaceuticals Bioreactors Callosities Cell Lines Cells Cytokinins Differentiations, Cell Ethanol Gelrite Gynostemma pentaphyllum Hyperostosis, Diffuse Idiopathic Skeletal Plant Cells Plant Growth Regulators Plants Sodium Hypochlorite Sucrose

Sterilization and Growth of Tomato Seedlings

The seeds of S. lycopersicum L. (Arka Vikas cultivar) were obtained from the Indian Institute of Horticulture Research (IIHR, Bangalore, India). The seeds were surface-sterilized for 5 min with 70% ethanol and seeds were resuspended in 4% sodium hypochlorite (v/v) for 10 min before being rinsed three times with sterile distilled water. The sterile seeds were grown for 10–12 days in half-strength MS (Murashige and Skoog) media supplemented with 3% sucrose and 0.3% Gelrite (Sigma-Aldrich, St. Louis, MO, USA) at 25–28 °C and 70% relative humidity (RH) in a plant growth chamber with a 16/8 h light-dark photoperiod. Hypocotyls that were well-grown and healthy were used in the experiment.

Debbarma J., Saikia B., Singha D.L., Das D., Keot A.K., Maharana J., Velmurugan N., Arunkumar K.P., Reddy P.S, & Chikkaputtaiah C. (2023). CRISPR/Cas9-Mediated Mutation in XSP10 and SlSAMT Genes Impart Genetic Tolerance to Fusarium Wilt Disease of Tomato (Solanum lycopersicum L.). Genes, 14(2), 488.

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Publication 2023

Ethanol Gelrite Humidity Hypocotyl Light Plant Development Plant Embryos Sodium Hypochlorite Sterility, Reproductive Sucrose

Tobacco Protoplast Micro-callus Formation

After 4 weeks of culture, the initial protoplast culture medium containing TSA, NaB or Aza was replaced with protoplast culture medium without TSA, NaB, or Aza. Tobacco cell colonies and micro-callus were observed under a microscope. To test the effects of TSA, NaB, and Aza on the formation of micro-callus from tobacco and lettuce mesophyll protoplasts, ImageJ software was used to quantify the micro-callus area and images of the micro-callus at 14 days after the treatments were performed. Images of micro-callus were also acquired using a Zeiss Primovert optical microscope. To quantify the micro-callus area, images of each experimental group from three biological replicates were analyzed. The protoplasts treated with each chemical were cultured at 25°C in the dark for 4 weeks; thereafter, 10 mL of the protoplast culture medium was added and the cultures were transferred to a 16 h light/8 h dark photoperiod (30 μmol/m² s) and further cultured at 25°C with shaking at 50 rpm. After 4 weeks of culture, the micro-callus were transferred to the culture medium (MS medium containing 30 g/L sucrose, 6 g/L Gelrite, 0.1 mg/L NAA, 0.5 mg/L BAP, pH 5.7) for callus proliferation. For callus proliferation, three independent replicate experiments were performed, with four Petri dishes in each replicate. After about 4 weeks of culture on the callus proliferation medium, the number of cell aggregates with a diameter greater than 0.5 mm was counted using an automatic callus/colony counter (Quantica 500, Bioavlee).
To evaluate the effect of TSA on adventitious shoot formation in tobacco, protoplast-derived calluses were transferred to solid shoot induction medium. The shoot induction medium consisted of MS (Murashige and Skoog, 1962) with 0.4 mg/L thiamine HCl, 100 mg/L myo-inositol, 30 g/L sucrose, 2 mg/L Indole-3-acetic acid, and 1 mg/L BAP (pH 5.7). After 4 and 5 weeks of culture, the frequency of shoot formation (percentage of explants that formed adventitious shoots) was calculated. All culture combinations were evaluated in three replicates, and at least ten explants per Petri dish were analyzed per replicate.

Choi S.H., Ahn W.S., Lee M.H., Jin D.M., Lee A., Jie E.Y., Ju S.J., Ahn S.J, & Kim S.W. (2023). Effects of TSA, NaB, Aza in Lactuca sativa L. protoplasts and effect of TSA in Nicotiana benthamiana protoplasts on cell division and callus formation. PLOS ONE, 18(2), e0279627.

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Publication 2023

Biopharmaceuticals Callosities Cells DNA Replication Gelrite Hyperostosis, Diffuse Idiopathic Skeletal indoleacetic acid Inositol Lactuca sativa Light Light Microscopy Microscopy Nicotiana Protoplasts Sucrose thiamine hydrochloride

Top products related to «Gelrite»

Gelrite by Duchefa Biochemie

Sourced in Netherlands

Gelrite is a gelling agent used in the preparation of culture media for microbiological and plant tissue culture applications. It is a polysaccharide derived from the bacterium Sphingomonas elodea. Gelrite forms a transparent, rigid gel that provides a stable, supportive matrix for the growth and development of various biological specimens.

Gelrite by Merck Group

Sourced in United States

Gelrite is a gelling agent used in the preparation of culture media for various applications in microbiology and plant tissue culture laboratories. It is a powdered gellan gum that forms a clear, firm gel when dissolved in water or aqueous solutions.

Gelrite by Fujifilm

Sourced in Japan

Gelrite is a high-quality laboratory gelling agent used in various applications, including microbiology, tissue culture, and plant biotechnology. It is a natural polysaccharide that forms a transparent, firm gel when mixed with water or aqueous solutions. Gelrite is known for its reproducible gelling properties, making it a reliable choice for researchers and scientists.

Gelrite gellan gum by Merck Group

Sourced in Italy

Gelrite® gellan gum is a high-quality polysaccharide produced by the fermentation of Sphingomonas elodea. It is a natural, plant-based gelling agent used in various laboratory applications. Gelrite® forms firm, transparent gels that are thermally reversible and resistant to degradation by enzymes.

Gelrite agar by Duchefa Biochemie

Gelrite agar is a gelling agent used in microbiological and plant tissue culture applications. It is a high-purity polysaccharide derived from the bacterium Sphingomonas paucimobilis. Gelrite agar forms a transparent, firm gel at low concentrations and is commonly used as a substitute for traditional agar in various laboratory procedures.

Gelzan cm gelrite by Merck Group

Gelzan CM Gelrite is a powdered, high-purity gellan gum product manufactured by Merck Group. It is a microbial polysaccharide that can be used as a gelling agent and thickener in various applications.

Dimethyl sulfoxide (dmso) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh

DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

Ms basal salt mixture by Duchefa Biochemie

MS) basal salt mixture is a powdered mixture of inorganic salts and nutrients commonly used as a base medium for plant cell and tissue culture applications. Its core function is to provide the essential mineral elements required for plant growth and development in a controlled and standardized environment.

Gelrite by Research Products International

Sourced in United States

Gelrite is a gelling agent used in various laboratory applications. It is a microbial polysaccharide that can be used to create solid or semi-solid media for culturing microorganisms, plant tissue culture, and other scientific experiments. Gelrite forms transparent, thermoreversible gels that provide a stable support matrix for various applications.

Tissue culture flasks by BD

Sourced in United States, Germany, United Kingdom

Tissue culture flasks are laboratory containers designed for the in vitro cultivation of cells, tissues, or organisms. They provide a controlled environment for cell growth and experimentation, facilitating the study and manipulation of biological samples.

More about "Gelrite"

Gelrite, a bacterial polysaccharide also known as Gelrite® gellan gum or Gelzan CM Gelrite, is a popular gelling agent used in plant tissue culture media.
This thermoreversible gel provides a semi-solid support for plant growth and development, exhibiting excellent thermal stability and transparency.
Researchers can leverage AI-powered tools like PubCompare.ai to efficiently locate and compare the best Gelrite protocols from published literature, preprints, and patents, optimizing their experimental procedures and driving their discoveries forward.
Gelrite is commonly used as an alternative to traditional agar, offering advantages such as improved clarity and reproducibility.
It can be combined with MS (Murashige and Skoog) basal salt mixture to create a nutrient-rich, semi-solid culture medium for a variety of plant species.
The use of Gelrite in tissue culture flasks allows for controlled cultivation of plant samples, enabling researchers to study plant growth and development in a controlled environment.
Additonally, the use of DMSO (dimethyl sulfoxide) can enhance the solubility and performance of Gelrite in certain applications.
By leveraging AI-assisted tools like PubCompare.ai, researchers can optimize their Gelrite-based protocols, leading to more accurate and reproducible results in their plant research and discovery efforts.