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Gossypol

Gossypol is a polyphenolic compound found in cotton plants.
It has been studied for its potential medicinal properties, including antifertility, anticancer, and antimalarial effects.
Gossypol research can be optimized using the AI-driven platform PubCompare.ai, which helps researchers locate the best protocols and products by comparing data across literature, preprints, and patents.
This streamlines the research process and improves reproducibility.
PubCompare.ai is a valuable tool for scientists investigating the therapeutic applications of gossypol.

Most cited protocols related to «Gossypol»

1
Prostate Cancer Cell Line Cultivation
Cited 4 times
Cell culture reagents and fetal bovine serum (FBS) were purchased from HyClone (Waltham, MA). Human prostate cancer cell lines were obtained from American Type Culture Collection except the LNCaP-derived sublines CL-1 and C4-2B. Androgen-independent prostate cancer cell line CL-1 was derived from its parental androgen-dependent cells LNCaP and was provided by Dr. Arie Belldegrun at University of California - Los Angeles 38 (link). C4-2B cell line was provided by Dr. Kenneth Pienta at University of Michigan and maintained in T-medium supplemented with 10% (v/v) FBS and antibiotics. LNCaP, CL-1, PC-3 and DU-145 cells were cultured in DMEM Nutrient Mixture supplemented with 10% FBS and antibiotics. Normal human prostate epithelial cell line PrEC was purchased from Clonetics and maintained in PrEBM (Cambrex). (-)-Gossypol was purified from natural racemic gossypol as we previously described 14 (link), 39 , and dissolved in DMSO at 20mM as stock solution. 3-methyl adenine (3-MA), rapamycin and acridine orange were from Sigma-Aldrich (Louis, MO); caspase-3/CPP32 fluorometric assay kit was from Biovision (Mountain view, CA); antibodies against microtubule-associated protein 1 light chain 3 (LC3), PARP, Atg5, Atg10, Bcl-xL was from Cell Signaling (Boston, MA) and the antibodies against Bcl-2, Mcl-1, and Beclin1 were from Santa cruz (Santa cruz, CA). The antibody against PIK3C3 was from AbCam (Cambridge, MA). LC3 cDNA was kindly provided by Drs. N. Mizushima and T. Yoshimori at Osaka University, Japan. Beclin1 and Bcl-2 expression vectors were purchased from OriGene (Rockvill, MD).
Lian J., Wu X., He F., Karnak D., Tang W., Meng Y., Xiang D., Ji M., Lawrence T.S, & Xu L. (2010). A natural BH3 mimetic induces autophagy in apoptosis-resistant prostate cancer via modulating Bcl-2–Beclin1 interaction at endoplasmic reticulum. Cell Death and Differentiation, 18(1), 60-71.
Publication 2010
3-methyladenine Acridine Orange Androgens Antibiotics Antibodies BCL2 protein, human BECN1 protein, human Biological Assay CASP3 protein, human Caspase 3 Cell Culture Techniques Cell Lines Cells Cloning Vectors DNA, Complementary Fetal Bovine Serum Fluorometry Gossypol Homo sapiens Immunoglobulin Light Chains Immunoglobulins Light LINE-1 Elements Microtubule-Associated Protein 1 Nutrients Parent Prostate Prostate Cancer Prostatic Cancer, Castration-Resistant Sirolimus Sulfoxide, Dimethyl
2
Cell Viability Assay with MTT and WST-1
Cited 4 times
Cell viability was determined by the MTT-based assay using Cell Proliferation Reagent WST-1 (Roche, Basel, Switzerland) according to the manufacturer’s instruction. Cells (5000 cells/well) were plated in 96-well culture plates, and various concentrations of (-)-gossypol or docetaxel were added to the cells in triplicates. Four days later, WST-1 was added to each well and incubated for 1.5 hours at 37°C. Absorbance was measured with a plate reader at 450 nm with correction at 650 nm. The results are expressed as the % of absorbance of treated wells versus that of vehicle control. IC50, the drug concentration causing 50% growth inhibition, was calculated via sigmoid curve fitting using GraphPad Prism 5.0 (GraphPad, Inc.).
Meng Y., Tang W., Dai Y., Wu X., Liu M., Ji Q., Ji M., Pienta K., Lawrence T, & Xu L. (2008). Natural BH3-mimetic (-)-gossypol chemosensitizes human prostate cancer via Bcl-xL inhibition accompanied by increase of Puma and Noxa. Molecular cancer therapeutics, 7(7), 2192-2202.
Publication 2008
Biological Assay Cell Proliferation Cells Cell Survival Docetaxel Gossypol Pharmaceutical Preparations prisma Psychological Inhibition Sigmoid Colon
3
Evaluating Docetaxel and Gossypol Efficacy
Cited 4 times
Double-blinded in vivo experiments were carried out with 5-to-6-week old male athymic NCr-nu/nu nude mice purchased from NCI. After alcohol preparation of the skin, mice were inoculated s.c. with 0.1 ml of PC-3 cell suspension (5 × 106 cells) on both flanks using a sterile 22-gauge needle. When tumors reached 100 mm3, the mice were randomized into 6 groups with 5 to 8 mice per group. Group 1 was given carboxy methyl cellulose as vehicle control; Group 2 was given docetaxel 7.5mg/kg, i.v. 1/week ×3 weeks; Group 3 and Group 4 were respectively given (-)-gossypol 10 mg/kg and 20 mg/kg p.o. q.d. 7 weeks, Group 5 and Group 6 were given a combination of docetaxel 7.5mg/kg, i.v. 1/week ×3 weeks and (-)-gossypol 10 mg/kg or 20 mg/kg p.o. q.d. 7 weeks. The tumor sizes and animal body weights were measured twice weekly. Three weeks after the first treatment, one mouse from each group was sacrificed and the tumors were dissected. Tumor tissues with a size of about 8 mm3 were prepared for Western Blot as described above. All animal experiments were done according to the protocol approved by University of Michigan Guidelines for Use and Care of Animals.
Meng Y., Tang W., Dai Y., Wu X., Liu M., Ji Q., Ji M., Pienta K., Lawrence T, & Xu L. (2008). Natural BH3-mimetic (-)-gossypol chemosensitizes human prostate cancer via Bcl-xL inhibition accompanied by increase of Puma and Noxa. Molecular cancer therapeutics, 7(7), 2192-2202.
Publication 2008
Animals Body Weight Carboxymethylcellulose Cells Docetaxel Ethanol Gossypol Males Mice, Nude Mus Needles Neoplasms PC 3 Cell Line Skin Sterility, Reproductive Thymic aplasia Tissues Western Blot
4
Colon Cancer Cell Lines and Gossypol Derivatives
Cited 3 times

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Publication 2015
alpha-Tubulin Antibiotics Antibodies Autophagy bafilomycin A1 Biological Assay Biological Factors Cancer of Colon Caspase 3 Cell Lines Cells Colon CTNNB1 protein, human Cyclin D1 Fetal Bovine Serum Glutamine Goat Gossypol Heterozygote Homo sapiens HT29 Cells Melia azedarach methyleneimine Mus Novus Oligonucleotide Primers Powder Rabbits Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Sulfoxide, Dimethyl Survivin Western Blot Western Blotting
5
Gossypol and Docetaxel in Prostate Cancer
Cited 3 times
Human prostate cancer cell lines PC-3, DU-145, LNCaP and human lung fibroblast cell line WI-38 were obtained from the American Type Culture Collection. PC-3 cells were routinely maintained in RPMI 1640 (HyClone, Logan, UT), while DU-145, LNCaP and WI-38 were maintained in DMEM (HyClone, Logan, UT), supplemented with 10% fetal bovine serum (FBS; HyClone, Logan, UT). Murine pro-B lymphoid cell line FL5.12 stably transfected with Bcl-xL or vector were kindly provided by Dr. Gabriel Nunez and were maintained in IMEM (GIBCO, Grand Island, NY) supplemented with 10% FBS and 10% WEHI-3B (D-)-conditional medium as a source of IL-3 (21 (link)). The cell cultures were maintained in a humidified incubator at 37°C and with 5% CO2. (-)-Gossypol was purified from natural racemic gossypol. Briefly, racemic gossypol was reacted with L-phenylalanine methyl ester hydrochloride overnight at room temperature and sodium bicarbonate was added to yield gossypol Schiff’s base as a yellow solid. After silica gel column chromatography purification, the solution of the resolved (±)-gossypol-phenylalanine methyl ester Schiff’s base was hydrolyzed by a mixture of tetrahydrofuran, glacial acetic acid and hydrochloric acid at room temperature for 2 hours. The solution was extracted with acetic ether 4 times, then washed and dried. The (-)-gossypol was collected by filtration and evaporation. Some of the (-)-gossypol used for initial in vitro studies was kindly provided by Dr. Shaomeng Wang of the University of Michigan and by Dr. Dajun Yang through the NCI RAID program. For in vitro experiments, (-)-gossypol was dissolved in DMSO at 20mM as a stock solution. For in vivo studies, (-)-gossypol was suspended in carboxy methyl cellulose and then sonicated for 30 min, mixed before each administration. Docetaxel (Taxotere, TXT) was purchased from Sanofi-Aventis (Bridgewater, NJ) and diluted in PBS for in vivo studies.
Meng Y., Tang W., Dai Y., Wu X., Liu M., Ji Q., Ji M., Pienta K., Lawrence T, & Xu L. (2008). Natural BH3-mimetic (-)-gossypol chemosensitizes human prostate cancer via Bcl-xL inhibition accompanied by increase of Puma and Noxa. Molecular cancer therapeutics, 7(7), 2192-2202.
Publication 2008
Acetic Acid Bicarbonate, Sodium Carboxymethylcellulose Cell Culture Techniques Cell Lines Chromatography Cloning Vectors Docetaxel Ethyl Ether Fibroblasts Filtration Gel Chromatography Gossypol Homo sapiens Hydrochloric acid Lung Lymph Mus PC 3 Cell Line phenylalanine methyl ester Pro-B Lymphocytes Schiff Bases Silica Gel Silicon Dioxide Sulfoxide, Dimethyl tetrahydrofuran Vinegar

Most recents protocols related to «Gossypol»

1
Estrogen Signaling in Breast Cancer Cells
Cells were grown in DMEM containing 10% charcoal-stripped FBS until 80% confluence, followed by treatments with phosphate buffered saline (PBS; control) or 5 or 10 nM β-estradiol (E2; Sigma-Aldrich; #E1024) in PBS for 4 h. For E2 treatment in the MCF7 cell line, both PBS and E2-PBS buffers contained 20 nM MLN4924 (a small molecule inhibitor of the Nedd8 activating enzyme) (Sigma-Aldrich; # 5054770001). After two washes with ice-cold PBS, the cells were used for RNA and protein isolation. The T47D cells were also cultured in DMEM containing 10% charcoal-stripped FBS and then treated with PBS, 100 nM bufalin (Sigma-Aldrich; #B0261) in PBS, or 5 μM gossypol (Sigma-Aldrich; #PHL83856) in PBS for 6 h, followed by additional treatment with or without 10 nM E2 for another 4 h. The treated cells were washed twice with ice-cold PBS buffer and used for RNA and protein isolation.
Wang J, & Zhou Z. (2023). Estrogen-dependent activation of NCOA3 couples with p300 and NF-κB to mediate antiapoptotic genes in ER-positive breast cancer cells. Discover. Oncology, 14, 28.
Full text: Click here
Publication 2023
bufalin Buffers Cell Lines Cells Charcoal Common Cold Enzyme Inhibitors Estradiol Gossypol isolation MCF-7 Cells MLN4924 NEDD8 protein, human Phosphates Proteins Saline Solution
2
In Vivo Evaluation of NCOA3-p300-NF-κB Depletion
Animal experiments were performed following a protocol (NCU202019BM) reviewed and approved by the Institutional Animal Care and Use Committee of Jiangxi Provincial People’s Hospital. The in vivo effects of depletion of NCOA3-p300-NF-κB members were determined by mixing 5.0 × 105 Control-KD1, RelA-KD1, NFKB1-KD1, p300-KD1, and NCOA3-KD1 cells (all in T47D background) in 100 μL of PBS with 50% Matrigel (Sigma-Aldrich; #CLS354234) and inoculating them subcutaneously into one armpit of female BALB/c nude mice (22–25 g) (n = 10 for each group) (Vital River Laboratories, Beijing, China). Each group of mice was randomly divided into two subgroups. One mouse subgroup was implanted with a 0.18 mg E2 pellet (Innovative Research Of America, Sarasota, FL, USA; #NC1775204) following a previous protocol [21 (link)]. The other group underwent the same surgery but without E2 pellet implantation. The position of the E2 pellet implantation was contralateral to the cell injection site between the skin and the peritoneal wall. Tumor sizes were measured every 5 days with a digital caliper (Thermo Fisher; #0666416), and tumor volumes were calculated using the formula: volumes = ½ (Length × Width2). For evaluation of the in vivo effects of NCOA3 inhibitors, T47D cells (5.0 × 105) were injected into female BALB/c nude mice (n = 60), and half of the mice were further implanted with a 0.18 mg E2 pellet. After the tumor volumes reached approximately 150 mm3, the mice were randomly grouped and injected intraperitoneally every 5 days with 1.5 mg/kg bufalin (n = 10), 50 mg/kg gossypol (n = 10), or PBS (control, n = 10). Tumor sizes were measured with a digital caliper every 5 days.
Wang J, & Zhou Z. (2023). Estrogen-dependent activation of NCOA3 couples with p300 and NF-κB to mediate antiapoptotic genes in ER-positive breast cancer cells. Discover. Oncology, 14, 28.
Full text: Click here
Publication 2023
ACTR protein, human Axilla bufalin Cells EP300 protein, human Gossypol inhibitors Institutional Animal Care and Use Committees matrigel Mice, Inbred BALB C Mice, Nude Mus Neoplasms NF-kappa B Operative Surgical Procedures Ovum Implantation Peritoneum RELA protein, human Rivers Skin Woman
3
Replacing Fish Meal with Cottonseed Meal in Aquafeeds
Using FM (crude protein, 73.6%) and CSM (crude protein, 49.6%; free gossypol, 1.12 mg/g) as the primary protein sources, five isonitrogenous (41% crude protein) and isocaloric (21 kJ/g gross energy) diets (C0, C8.5, C17.2, C25.7, and C34.4) were prepared to replace 0%, 12%, 24%, 36%, and 48% FM protein with 0%, 8.5%, 17.2%, 25.7%, and 34.4% CSM, respectively. DL-methionine, L-lysine, and L-leucine were also supplemented to each diet to provide equal amounts of methionine, lysine, and leucine in all experimental diets. The feed ingredients and proximate composition of experimental diets are listed in Table 1, and the corresponding amino acid profiles are shown in Table 2.
All feed ingredients except lipid sources were crushed and passed through a sieve with a diameter of 320 μm. Soybean lecithin was preblended in the mixture of soybean oil and fish oil. All ingredients were thoroughly mixed; the lipid mixture was added, and then thoroughly mixed again. Suitable water was added to make a dough, and the dough was squeezed into 1.5-mm pellet by a pellet feed maker (KS-180; Jiangsu Jingu Rice Mill Co., Ltd., Zhenjiang, China). The wet pellets were dried at 40°C and then stored at −20°C until use.
Li B., Su L., Sun Y., Huang H., Deng J, & Cao Z. (2023). Evaluation of Cottonseed Meal as an Alternative to Fish Meal in Diet for Juvenile Asian Red-Tailed Catfish Hemibagrus wyckioides. Aquaculture Nutrition, 2023, 1741724.
Full text: Click here
Publication 2023
Amino Acids Diet G-substrate Gossypol GTP-Binding Proteins Lecithin Leucine Lipids Lysine Methionine Oils, Fish Oryza sativa Pellets, Drug Proteins Racemethionine Soybean oil Soybeans
4
Proximate Composition Analysis of Feed
The proximate composition of feed ingredients, experimental diets, and whole-body samples were measured following the method of AOAC [22 ]. Dry matter was determined by drying the sample to a constant weight at 105°C; crude protein content was examined by the Kjeldahl method (N × 6.25); crude lipid content was determined using the Soxhlet method with ether extraction; crude ash content was determined after burning in a muffle furnace at 550°C for 6 h. A bomb calorimeter (Parr 1351; Parr Instrument Co., Moline, IL, USA) was used to measure gross energy. Free gossypol content in diets was determined by high-performance liquid chromatography [23 (link)]. The amino acid composition of diets was determined using the method described by Mai et al. [24 (link)].
Li B., Su L., Sun Y., Huang H., Deng J, & Cao Z. (2023). Evaluation of Cottonseed Meal as an Alternative to Fish Meal in Diet for Juvenile Asian Red-Tailed Catfish Hemibagrus wyckioides. Aquaculture Nutrition, 2023, 1741724.
Full text: Click here
Publication 2023
Amino Acids Diet Ethyl Ether Gossypol High-Performance Liquid Chromatographies Human Body Lipids Proteins
5
Virus-Induced Gene Silencing for Gossypol Analysis
The online tool SGN VIGS (https://vigs.solgenomics.net/) was used to design the appropriate silencing region in the target gene [36 (link)]. The parameters were set as follows: n-mer size is 21, fragment length is 300, and mismatches is 0. The specific parameters could be adjusted according to the score. DNAMAN8 was used for restriction enzyme analysis, the target fragment was ligated to TRV2 (tobacco rattle virus) vector through EcoRI and BamHI digestion sites, and the recombinant vector was constructed into the competent cells of Agrobacterium tumefaciens GV3101 by freeze–thaw method. The bacterium GV3101 containing pTRV1 vector was used as auxiliary bacteria, TRV1/GV3101 and TRV2/GV3101 were mixed in a 1:1 ratio and injected into the cotyledons of 8-day-old cotton seedlings until the leaves were filled and incubated in the dark for 24 h, at least 30 cotton seedlings were injected for each gene. After 30 days, the leaves were harvested to measure the gossypol content.
Ye K., Teng T., Yang T., Zhao D, & Zhao Y. (2023). Transcriptome analysis reveals the effect of grafting on gossypol biosynthesis and gland formation in cotton. BMC Plant Biology, 23, 37.
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Publication 2023
Agrobacterium tumefaciens Bacteria Cells Cloning Vectors Cotyledon Deoxyribonuclease EcoRI Digestion Freezing Genes Gene Silencing Gossypium Gossypol Restriction Mapping Seedlings Tobacco rattle virus

Top products related to «Gossypol»

1
Gossypol by Merck Group
Sourced in United States, China, Macao, Germany
Gossypol is a chemical compound found in the cotton plant. It is a crystalline compound that has the molecular formula C30H30O8. Gossypol serves as a core component in various laboratory applications and research studies.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
4
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
5
Propidium iodide by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Macao, Canada, Spain, India, Belgium, Australia, Israel, Switzerland, Poland, Ireland, Argentina, Austria, Brazil, Sweden, Portugal, New Zealand, Netherlands, Slovakia, Norway, Hungary, Czechia, Denmark
Propidium iodide is a fluorescent dye commonly used in molecular biology and flow cytometry applications. It binds to DNA and is used to stain cell nuclei, allowing for the identification and quantification of cells in various stages of the cell cycle.
6
Cycloheximide (chx) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Japan, Spain, Canada, Switzerland, India, Israel, Brazil, Poland, Portugal, Australia, Morocco, Sweden, Austria, Senegal, Belgium
Cycloheximide is a laboratory reagent commonly used as a protein synthesis inhibitor. It functions by blocking translational elongation in eukaryotic cells, thereby inhibiting the production of new proteins. This compound is often utilized in research applications to study cellular processes and mechanisms related to protein synthesis.
7
Rpmi 1640 by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Canada, Japan, Australia, Italy, Switzerland, Belgium, New Zealand, Austria, Netherlands, Israel, Sweden, Denmark, India, Ireland, Spain, Brazil, Norway, Argentina, Macao, Poland, Holy See (Vatican City State), Mexico, Hong Kong, Portugal, Cameroon
RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.
8
Gossypol by MedChemExpress
Sourced in United States
Gossypol is a natural polyphenolic compound. It is a small molecule with a molecular weight of 518.58 g/mol. Gossypol exhibits a range of biological activities and can be used as a research tool.
9
Cellquest software by BD
Sourced in United States, Germany, United Kingdom, Italy, Canada, China, Japan, Belgium, France, Spain, Switzerland, Poland, Uruguay, Denmark, Singapore
CellQuest software is a data acquisition and analysis software designed for flow cytometry applications. It provides tools for acquiring, processing, and analyzing flow cytometry data.
10
Facscalibur by BD
Sourced in United States, Germany, United Kingdom, China, Canada, Japan, Italy, France, Belgium, Switzerland, Singapore, Uruguay, Australia, Spain, Poland, India, Austria, Denmark, Netherlands, Jersey, Finland, Sweden
The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.

More about "Gossypol"

Gossypol is a natural polyphenolic compound derived from the cotton plant.
This versatile molecule has garnered significant attention for its potential therapeutic applications, including its documented antifertility, anticancer, and antimalarial properties.
Researchers investigating the medicinal uses of gossypol can leverage the AI-driven platform PubCompare.ai to streamline their research process and improve reproducibility.
PubCompare.ai is designed to help scientists locate the best protocols and products for gossypol research by comparing data across published literature, preprints, and patent information.
This comprehensive, data-driven approach can save valuable time and resources, while also enhancing the reliability of research findings.
In addition to gossypol, researchers may also utilize other common cell culture reagents and techniques, such as fetal bovine serum (FBS), dimethyl sulfoxide (DMSO), Dulbecco's Modified Eagle Medium (DMEM), propidium iodide, cycloheximide, and RPMI 1640 medium.
Flow cytometry analysis using a FACSCalibur instrument and CellQuest software can also provide valuable insights into the effects of gossypol on cell viability and apoptosis.
By leveraging the power of PubCompare.ai and integrating these well-established cell culture methods, scientists can optimize their investigations into the therapeutic potential of this fascinating natural compound, leading to breakthroughs in areas like reproductive health, cancer treatment, and malaria prevention.