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Harmine

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PubCompare.ai's AI-driven platfrom can help streamline and optimie harmine research by providing access to protocols from literature, preprints, and patents, and using AI-based comparisons to identify the best protocols and products. This can enhance reproducibility and efffeciency in harmine studies.

Harmine is a naturally occurring beta-carboline alkaloid found in various plant species, including Peganum harmala.
It has been studied for its potential therapeutic applications in the fields of neuroscience, psychiatry, and oncology.
Harmine has been investigated for its effects on the central nervous system, including its ability to modulate monoamine oxidase activity and influence neurotransmitter levels.
Additionally, research has explored harmine's anti-inflammatory, antioxidant, and antitumor properties.
PubCompare.ai's AI-driven platform can help streamline and optimize harmine research by providng access to protocols from literature, preprints, and patents, and using AI-based comparisons to identify the best protocols and prodcuts.
This can enhance reproducibility and efficiency in harmine studies.

Most cited protocols related to «Harmine»

Quantifying Human Beta Cell Responses

Cited 5 times

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Publication 2018

accutase Adenovirus Infections Adenovirus Vaccine Cells DAPI Fetal Bovine Serum Flow Cytometry Harmine Homo sapiens Human Embryonic Stem Cells Human Herpesvirus 5 Insulin Islets of Langerhans Laminin Lysine NRG1 protein, human Pancreatic beta Cells Pharmaceutical Preparations Poly A Stem Cells Sulfoxide, Dimethyl Trypsin

MALDI-FT-ICR Mass Spectrometry Protocol

Cited 4 times

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Publication 2008

2,3-dihydroxybenzoic acid acetonitrile Cells Exanthema Harmine indole Nitrogen Pulses Sodium Chloride Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Stainless Steel Vacuum

Endothelial BRG1 Deletion and Liver Fibrosis

Cited 3 times

All animal experiments were reviewed and approved by the intramural Nanjing Medical University Ethics Committee on Humane Treatment of Experimental Animals. All mice were bred at the Nanjing Biomedical Research Institute of Nanjing University (NBRI). Endothelial-specific deletion of BRG1 was achieved by crossing the Smarca4^f/f strain (Li et al., 2018b (link)) to the Cdh5-Cre^ERT2 strain (Li et al., 2018c (link)). To delete BRG1, 6-week male Smarca4^f/f; Cdh5-Cre^ERT2 mice were injected with Tamoxifen (1mg/kg) daily for 5 consecutive days; the age- and sex-matched control mice (Smarca4^f/f) received the same injection regimen. Liver fibrosis was induced in mice by CCl₄ injection (1.0 mL/kg body weight as 50%, vol/vol, weekly for 6 weeks) as previously described (Fan et al., 2015 (link); Tian et al., 2015 (link)). The HIF1α inhibitors (LW-6, 20 mg/kg; YC-1, 30 mg/kg) were injected peritoneally every other day. The TWIST inhibitor (Harmine, 10 mg/kg) was injected peritoneally every other day.

Dong W., Kong M., Zhu Y., Shao Y., Wu D., Lu J., Guo J, & Xu Y. (2020). Activation of TWIST Transcription by Chromatin Remodeling Protein BRG1 Contributes to Liver Fibrosis in Mice. Frontiers in Cell and Developmental Biology, 8, 340.

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Publication 2020

Animals Body Weight CCL4 protein, human CDH5 protein, human Deletion Mutation Endothelium Ethics Committees Fibrosis, Liver Harmine HIF1A protein, human inhibitors Males Mice, House SMARCA4 protein, human Strains Tamoxifen Therapies, Investigational Treatment Protocols

Radiochemical Synthesis and Quality Control of [11C]Harmine

Cited 3 times

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Publication 2015

Communicable Diseases Endotoxins Europeans Gamma Rays Harmaline Harmine harmol High-Performance Liquid Chromatographies Physical Examination Radioactivity Radioisotopes Radiopharmaceuticals Retention (Psychology) Sterility, Reproductive

Angiogenesis Assays Using Rat Aortic Rings and Mouse Corneal Micropockets

Cited 3 times

A rat aortic ring assay was performed as described elsewhere [25] (link) with some modification. Briefly, thoracic aortas were sliced into rings of 1–1.5 mm in circumference. VEGF (200 ng) with or without harmine in serum-free MCDB131 medium was added to aortic rings coated with Matrigel. Medium was exchanged every 2 days. On day 7, microvessel outgrowths were photographed and analyzed. A mouse corneal micropocket assay was performed as described elsewhere [26] (link) with some modification. Briefly, slow-release pellets (0.35 × 0.35 mm) containing 200 ng VEGF with or without 5 µg harmine were prepared with a sucrose octasulfate-aluminum complex and poly-HEMA. A corneal micropocket was created in the eye of a 4-5-week-old C57BL/6 mouse with a modified needle, and pellets were implanted into these micropockets. Chlortetracycline hydrochloride ophthalmic ointment was applied to each operated eye to prevent infection. After 7 days, the vessel length and clock hours of new blood vessels were examined under a stereomicroscope and recorded. The area of neovasculature was calculated according to the formula: Area (mm²) = 0.2 × π × VL (mm) × CN (mm), where VL is the maximal vessel length extended from the limbal vasculature toward the pellet and CN is the clock hours of neovascularization, 1 clock hour equals 30 degrees of arc.

Dai F., Chen Y., Song Y., Huang L., Zhai D., Dong Y., Lai L., Zhang T., Li D., Pang X., Liu M, & Yi Z. (2012). A Natural Small Molecule Harmine Inhibits Angiogenesis and Suppresses Tumour Growth through Activation of p53 in Endothelial Cells. PLoS ONE, 7(12), e52162.

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Publication 2012

Aluminum Aorta Biological Assay Blood Vessel Cornea Harmine Hydrochloride, Chlortetracycline Infection matrigel Mice, House Mice, Inbred C57BL Microvessels Needles Ointments Pathologic Neovascularization Pellets, Drug Polyhydroxyethyl Methacrylate Serum sucrose octasulfate Thoracic Aorta Vascular Endothelial Growth Factors

Most recents protocols related to «Harmine»

Molecular Docking of Compound 3H

The molecular docking study was performed by using autodock 4.2 software package³⁹ (link). Firstly, the crystal structures of MAO-A (PDB ID: 2z5x) and MAO-B (PDB ID: 2v5z) were obtained from PDB website (https://www.rcsb.org/). Then, original ligands (harmine or safinamide) and water molecules were removed from the crystals, and the polar hydrogens were added to the obtained crystals. Subsequently, the chemical structure of 3h was plotted using chemdraw software, and then the lowest energy conformation of 3h was calculated in Chem3D and its torsion tree was defined in autodock 4.2 software. After preparation of macromolecules and ligand, the ligand was embed into the macromolecular receptor using autogrid. After the embedment, the autodock program was run, and the flexible docking program generally runs 100 times. The conformation with the highest populated cluster and lowest energy was chosen to the result discussion.

Cao Z., Wang X., Zhang T., Fu X., Zhang F, & Zhu J. (2023). Discovery of novel 2-(4-(benzyloxy)-5-(hydroxyl) phenyl) benzothiazole derivatives as multifunctional MAO-B inhibitors for the treatment of Parkinson’s disease. Journal of Enzyme Inhibition and Medicinal Chemistry, 38(1), 2159957.

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Publication 2023

Harmine Hydrogen Ligands MAOA protein, human Monoamine Oxidase B safinamide Trees

Preparing and Evaluating Proteasome Inhibitors

Harmine (Tocris 5075), LDN-192960 (Sigma-Millipore SML0755), KRIBB11 (Tocris 5480), bortezomib (Selleckchem S1013), carfilzomib (Selleckchem S2853), ixazomib (Selleckchem S2180), and oprozomib (Selleckchem S7049) were dissolved in DMSO at a stock concentration of 10 mM and treatments were carried out as indicated. Curcumin (Sigma-Millipore 08511) was diluted in DMSO at a stock concentration of 5 mM in the dark and prepared fresh prior to each experiment and the excess solution was never stored. Curcumin treatment at a final concentration of 5 µM was always carried out in media containing either 1% bovine serum albumin (BSA) or 10% fetal bovine serum (FBS) to maintain maximum stability and to avoid aggregation [15 (link),19 (link)].

Tandon V., Moreno R., Allmeroth K., Quinn J., Wiley S.E., Nicely L.G., Denzel M.S., Edwards J., de la Vega L, & Banerjee S. (2023). Dual inhibition of HSF1 and DYRK2 impedes cancer progression. Bioscience Reports, 43(1), BSR20222102.

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Publication 2023

Bortezomib carfilzomib Curcumin Fetal Bovine Serum Harmine ixazomib KRIBB 11 oprozomib Serum Albumin, Bovine Sulfoxide, Dimethyl

Evaluating Cell Viability and Invasion in 3D

Cell viability assays were carried out with or without 48–72 h treatment of indicated drugs or DMSO control using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay kit (Promega), following manufacturer’s instructions and data were represented as %viability compared with DMSO-treated control. The 3D Matrigel invasion assays were performed using 8 μm pore size transwells coated with Matrigel™ (BD Biosciences) as described previously [22 (link)]. The bottom chamber contained normal growth media (DMEM with 10% FBS with or without 5 μM curcumin or 10 μM harmine) as a chemoattractant. MDA-MB-231 cells were seeded into the upper chamber (20000 cells/insert) in DMEM with 1% BSA with or without 5 μM curcumin or 10 μM harmine. After 24 h of culture, cells that migrated through the matrix were quantified using Cyquant following manufacturer’s instructions (Life Technologies).

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Publication 2023

Biological Assay Cell Proliferation Cells Cell Survival Chemotactic Factors Culture Media Curcumin Harmine matrigel MDA-MB-231 Cells Pharmaceutical Preparations Promega Radioactivity Sulfoxide, Dimethyl

Antiviral Potential of Indole and Carboline Alkaloids

The tested compounds used in our investigation are listed, in detail (Table 1). Strychnine sulfate, reserpine, brucine, eserine, harmine, and norharmane were purchased from Sigma-Aldrich (Sigma-Aldrich, Germany). Harmane, harmaline, and harmalol were purchased from the Molekula group (Molekula, Germany). Methyl ergometrine was obtained from Novartis (Al-Amiria District, Cairo). Their documented biological activities/chemical nucleus served as the basis for their selection to be examined for their antiviral potential against IAVs. The molecular structures of the studied compounds are shown (Fig. 1).Table 1

The tested indole alkaloid and β-carboline indole alkaloid compounds.

Compound	Class	Biological activities	Viruses	Citation
Strychnine Sulfate	Indole Alkaloids	Antitumor	N/A	^{24 (link)}
Reserpine	Indole Alkaloids	Anti-inflammatory, antihypertensive, and antiviral activities	SARS-CoV	^{15 (link),25 (link),26 (link)}
Brucine	Indole Alkaloids	Anticarcinogenic, neurotoxic, anti-inflammatory, and analgesic	N/A	^{27 (link)–29 (link)}
Methyl ergometrine	Indole Alkaloids	Antihemorrhagic	N/A	^{30 (link)}
Eserine	Indole Alkaloids	Anticholinesterase and antiviral	SARS-CoV-2	^{14 (link),31 (link)}
Harmine	β-carboline Indole Alkaloids	Anticarcinogenic, antiviral antifungal, antimicrobial, antiplasmodial, and antioxidant	HSV-2, DENV-2, and EV-71	^{17 (link)–20 (link)}
Harmalol	β-carboline Indole Alkaloids	Anticarcinogenic and antifungal	N/A	^{32 (link),33 (link)}
Harmane	β-carboline Indole Alkaloids	Antianxiety, antidepressant, antidiabetic, antioxidant, antiparasitic and antiviral	HSV-1 & HSV-2	^{34 (link)–36 (link)}
Harmaline	β-carboline Indole Alkaloids	Antioxidant and anti-tumor	N/A	^{37 (link),38 (link)}
Norharmane	β-carboline Indole Alkaloids	Anticancer and antibacterial	N/A	^{39 (link),40 (link)}

Figure 1

The molecular structures of the studied compounds. (1) Strychnine sulfate, (2) Reserpine, (3) Brucine, (4) Methyl ergometrine, (5) Eserine, (6) Harmine, (7) Harmalol, (8) Harmane, (9) Harmaline, (10) Norharmane.

Hegazy A., Mahmoud S.H., Elshaier Y.A., Shama N.M., Nasr N.F., Ali M.A., El-Shazly A.M., Mostafa I, & Mostafa A. (2023). Antiviral activities of plant-derived indole and β-carboline alkaloids against human and avian influenza viruses. Scientific Reports, 13, 1612.

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Publication 2023

Anti-Inflammatory Agents Antidepressive Agents Antidiabetics Antihypertensive Agents Antioxidants Antiparasitic Agents Antiviral Agents Biopharmaceuticals brucine Carbolines Cell Nucleus Ergonovine Eserine Harmaline harmalol harman Harmine Indole Alkaloids Inflammation Microbicides Molecular Structure Neurotoxicity Syndromes norharman Reserpine Strychnine Sulfates, Inorganic

RNA-Seq of DMSO, Harmine, and 1H3-Treated CD4+ T Cells

Mock-infected CD4⁺ T cells treated with DMSO, harmine (10 µM) or 1H3 (200 nM) for 3 days (two donors per treatment condition) were used for sample preparation for RNA-Seq. Total RNA was extracted from treated samples using Aurum Total RNA Mini Kit (Bio-Rad #7326820EDU) following the manufacturer’s instructions with the addition of Turbo DNase (Ambion). RNA integrity was verified using an Agilent Technologies 2100 Bioanalyzer (RNA integrity number value ≥ 8) at Donnelley Sequencing Center (DSC, University of Toronto, Toronto, ON, Canada). Libraries were generated from 500 ng of total RNA using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7490S). Polyadenylated mRNA enrichment was performed using the NEBNext Poly(A) mRNA Magnetic Isolation Module followed by slight modification on RNA fragmentation time (10 min at 94°C) for an insert size of 350 bp (to span exon-exon junction). The RNA fragments were then copied into cDNA using NEBNext First Strand Synthesis and Second Strand Synthesis modules, and the double-stranded cDNA was purified using SPRIselect beads. This was followed by end-prep of cDNA library and adapter ligation using NEBNext Ligation Enhancer and NEBNext Ultra II Ligation Master Mix. Adapter ligated DNA was then PCR enriched using NEBNext Ultra II Q5 Master Mix and NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) followed by purification of PCR enriched reaction using SPRIselect beads. The quality of the library was assessed on an Agilent Bioanalyzer DNA 1000 Chip, normalized and pooled for cluster generation.

Dahal S., Clayton K., Cabral T., Cheng R., Jahanshahi S., Ahmed C., Koirala A., Villasmil Ocando A., Malty R., Been T., Hernandez J., Mangos M., Shen D., Babu M., Calarco J., Chabot B., Attisano L., Houry W.A, & Cochrane A. (2023). On a path toward a broad-spectrum anti-viral: inhibition of HIV-1 and coronavirus replication by SR kinase inhibitor harmine. Journal of Virology, 97(10), e00396-23.

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Publication 2023

2',5'-oligoadenylate Anabolism CD4 Positive T Lymphocytes cDNA Library Deoxyribonucleases DNA, Complementary DNA Chips Donors Exons Harmine isolation Ligation mRNA, Polyadenylated RNA-Seq RNA II Sulfoxide, Dimethyl

Top products related to «Harmine»

Harmine by Merck Group

Sourced in United States, Germany, France

Harmine is a chemical compound that is used as a laboratory reagent. It is a crystalline solid that is soluble in organic solvents. Harmine has a molecular formula of C₁₃H₁₂N₂O and a molecular weight of 212.24 g/mol.

Harmaline by Merck Group

Sourced in United States, Germany

Harmaline is a chemical compound that serves as a fluorescent dye. It is used in various laboratory applications, including fluorescence microscopy and analytical techniques. The core function of Harmaline is to provide a fluorescent signal that can be detected and analyzed in controlled experimental settings.

Harmane by Merck Group

Sourced in United States, Germany

Harmane is a laboratory product manufactured by Merck Group. It is a chemical compound used in various analytical and research applications. This product serves as a core functional component in specialized laboratory equipment.

Formic acid by Merck Group

Sourced in Germany, United States, Italy, United Kingdom, France, Spain, China, Poland, India, Switzerland, Sao Tome and Principe, Belgium, Australia, Canada, Ireland, Macao, Hungary, Czechia, Netherlands, Portugal, Brazil, Singapore, Austria, Mexico, Chile, Sweden, Bulgaria, Denmark, Malaysia, Norway, New Zealand, Japan, Romania, Finland, Indonesia

Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.

Harmine hydrochloride by Merck Group

Sourced in United States

Harmine hydrochloride is a chemical compound that is commonly used as a research tool in laboratory settings. It is a naturally occurring alkaloid derived from various plant species. Harmine hydrochloride is primarily utilized for its inhibitory effects on certain enzymes and its potential applications in scientific investigations, but its specific functions and intended uses should not be extrapolated beyond the scope of its established research applications.

Harmine by Cayman Chemical

Sourced in United States

Harmine is a complex organic compound commonly used in research and laboratory settings. It serves as a valuable analytical standard and reference material for various scientific applications. The core function of harmine is to provide a consistent and reliable source for comparative analysis, method development, and quality control purposes.

Dimethyl sulfoxide (dmso) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh

DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Accutase by Merck Group

Sourced in United States, Germany, United Kingdom, France, Italy, Switzerland, Sao Tome and Principe, Macao, Denmark

Accutase is a laboratory reagent that is used for the enzymatic dissociation and harvesting of adherent cells in cell culture. It is a proprietary mixture of proteolytic and collagenolytic enzymes that effectively detaches cells from the culture surface without affecting cell viability or cell surface receptors.

Harmine by Bio-Techne

Harmine is a chemical compound produced by Bio-Techne. It is a selective and potent inhibitor of the enzyme monoamine oxidase A (MAO-A). Harmine's core function is to serve as a research tool for the study of MAO-A and its role in various biological processes.

What is Harmine and what are its potential therapeutic applications?

How can PubCompare.ai help optimize Harmine research?

What are the key benefits of using PubCompare.ai for Harmine research?

PubCompare.ai allows researchers to screen protocol literature more efficiently, and leverage AI to pinpoint critical insights. The platform can help identify the most effective protocols related to Harmine for your specific research goals. The AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy.

More about "Harmine"

Harmine is a naturally occurring beta-carboline alkaloid found in various plant species, including Peganum harmala.
This intriguing compound has been the subject of extensive research in the fields of neuroscience, psychiatry, and oncology, with scientists exploring its potential therapeutic applications.
One key area of focus has been the impact of harmine on the central nervous system.
Researchers have investigated its ability to modulate monoamine oxidase activity and influence neurotransmitter levels, which could have implications for the management of neurological and psychiatric conditions.
Beyond its effects on the brain, harmine has also demonstrated anti-inflammatory, antioxidant, and antitumor properties, making it a subject of interest in the realms of inflammation and cancer research.
The structurally similar compounds harmaline and harmane have also been studied for their potential biological activities.
To streamline and optimize harmine research, PubCompare.ai's AI-driven platform offers a valuable resource.
By providing access to protocols from literature, preprints, and patents, and utilizing AI-based comparisons to identify the best protocols and products, the platform can enhance reproducibility and efficiency in harmine studies.
This can be particularly useful when working with related compounds like harmaline, harmane, and formic acid, or when using common research materials like DMSO and FBS.
Ultimately, the versatility and potential of harmine make it a compound worthy of continued exploration, and PubCompare.ai's tools can be a valuable asset in navigating the complexities of this research area.
Whether you're studying the neurological, inflammatory, or oncological implications of harmine, the platform can help streamline your workflow and enhance the quality of your findings.