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Hemp
Hemp
Hemp, also known as Cannabis sativa, is a versatile plant with a wide range of applications.
It is cultivated for its fiber, seeds, and therapeutic compounds.
Hemp fibers can be used in textiles, paper, and construction materials, while the seeds are a nutritious source of protein, healthy fats, and other beneficial nutrients.
The plant also produces cannabinoids, such as cannabidiol (CBD), which have demonstrated potential for therapeutic uses, including pain management, anxiety reduction, and anti-inflammatory effects.
However, the legal status and regulation of hemp production and use varies across different regions and countries.
Researchers and healthcare professionals continue to explore the diverse applications and potential benefits of this remarkable plant.
It is cultivated for its fiber, seeds, and therapeutic compounds.
Hemp fibers can be used in textiles, paper, and construction materials, while the seeds are a nutritious source of protein, healthy fats, and other beneficial nutrients.
The plant also produces cannabinoids, such as cannabidiol (CBD), which have demonstrated potential for therapeutic uses, including pain management, anxiety reduction, and anti-inflammatory effects.
However, the legal status and regulation of hemp production and use varies across different regions and countries.
Researchers and healthcare professionals continue to explore the diverse applications and potential benefits of this remarkable plant.
Most cited protocols related to «Hemp»
Cannabinoids
Cannabis
Cannabis sativa
Climate
Females
Fibrosis
Flowers
Head
Hemp
Illicit Drugs
Males
Marijuana Abuse
N-nitrosoiminodiacetic acid
Plant Embryos
Plants
Resins, Plant
Solvents
Stem, Plant
Sterility, Reproductive
Thai
50 μg of protein were labelled with Cy-dyes. Following the labelling, the samples were handled as described in [65 (link)]. The analysis of the gel images was performed with the DeCyder™ software (GE Healthcare, v. 7.0.8.53). Spots were considered as significantly different when detected on at least 75% of analysed gel images, protein abundance with a minimum fold change of 1.5 with a Student’s t-test p-value below 0.05 [Additional file 3 ]. Following MALDI analysis, the mass spectra of digested peptides were identified by carrying out a MASCOT database search against our in-house hemp transcriptome database (170,598 sequences; 64,508,806 residues) annotated using Blast2GO PRO version 3.0 against the A. thaliana non-redundant database and the NCBI Viridiplantae database, with the following parameters: fixed modifications: carbamidomethyl (C); variable modifications: dioxidation (W), oxidation (HW), oxidation (M), Trp → kynurenin (W); mass values: monoisotopic; peptide mass tolerance: ± 100 ppm; fragment mass tolerance: ± 0.5 Da and Max number of missed cleavages: 2. Individual ions scores greater than 42 indicate identity or extensive homology (p < 0.05), protein scores greater than 65 are significant (p < 0.05). A protein was identified with only one peptide if the individual ion score was higher than 84. Principal Component Analysis (PCA) was performed with the DeCyder™ software.
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Cytokinesis
Dyes
Exanthema
Hemp
Immune Tolerance
Kynurenine
Mass Spectrometry
Peptides
Plants
Post-Translational Protein Processing
Proteins
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Staphylococcal Protein A
Student
Transcriptome
The marijuana strains genotyped in this study were provided by author DH (grown by Health Canada authorized producers) and represent germplasm grown and used for breeding in the medical and recreational marijuana industries (S2 Table ). Hemp strains were provided by author JV (Health Canada hemp cultivation licensee), and represent modern seed and fibre cultivars grown in Canada as well as diverse European and Asian germplasm (S3 Table ). DNA was extracted from hemp leaf tissue using a Qiagen DNeasy plant mini kit, and from marijuana leaves using a Macherey-Nagel NucleoSpin 96 Plant II kit with vacuum manifold processing. Library preparation and sequencing were performed using the GBS protocol published by Sonah et al [15 (link)]. The raw sequence has been deposited in the NIH Sequence Read Archive (SRA), under BioProject PRJNA285813. SNPs with a read depth of 10 or more were called using the GBS pipeline developed by Gardner et al. [16 (link)], aligning to the canSat3 C. sativa reference genome assembly [3 (link)]. Quality filtering of genetic markers was performed in PLINK 1.07 [17 (link)] by removing SNPs with (i) greater than 20% missingness by locus (ii) a minor allele frequency less than 1% and (iii) excess heterozygosity (a Hardy-Weinberg equilibrium p-value less than 0.0001). After filtering, 14,031 SNPs remained for analysis.
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Asian Persons
Cannabis
DNA Library
Europeans
Fibrosis
Genetic Markers
Genome
Hemp
Heterozygote
Plants
Single Nucleotide Polymorphism
Tissues
Vacuum
First, we aggregated 138 food items from the the FFQ into 30 food groups (in Table 1 ) based on their similarity in nutrients and earlier studies [15 (link)] in China.We then conducted the factor analysis (principal component) on the 30 food groups to identify dietary patterns. The obtained factors were rotated using orthogonal rotation so that the factors(dietary patterns) were uncorrelated with each other and were easier to interpret.The eigenvalue and scree plot were applied to decide which factors remained [16 (link)]. After evaluating the eigenvalues, the scree plot test, and interpretability, factors with an eigenvalues ≥ 2.0 were retained. Individual food items with a factor loading ≥ |0.4| were considered to significantly contribute to the pattern in this study. The labeling of dietary patterns was based on the interpretation of foods with high factor loadings for each dietary pattern [17 (link)]. Factor scores were categorized into quartiles (Q1 represented a low intake of the food pattern; Q4 represented a high intake of the food pattern).
Food grouping used in the dietary pattern analyses
| Food groups | Food items |
|---|---|
| Refined grains | Rice, porridge, rice in soup, noodles,instant noodles,steamed bun, wonton, dumplings,white breads, toasted bread |
| Whole grains | Corn, sorghum, millet, oats |
| Tubers | Sweet potato, potato, taro |
| Vegetables | Wild vegetables, green vegetable, spinach, green peppers, tomato Chinese cabbage, radish, cucumer, eggplant |
| Fruit | Apple,pears, peach, apricots, cherries, grapes, bananas, cantaloupe, watermelon, oranges, grapefruit, kiwi,strawberries and et al. |
| Pickled vegetables | Salted vegetables, Chinese sauerkraut |
| Mushrooms | Mushroom, shiitakes, enoki |
| Red meat | Pork, mutton, beef |
| Poultry and organs | Chicken, duck, liver, animal blood |
| Processed and cooked meat | Ham and sausage, sauced pork, roast duck |
| Fish and shrimp | Fish, shrimp |
| Eggs | Duck eggs, chicken eggs |
| Seafood | Sea fish, shrimp, crab,squid, jellyfis, shellfish |
| Bacon and salted fish | Salted meat and duck, salted fish |
| Salted and preserved eggs | Salted duck and chicken eggs, preserved eggs |
| milk | Liquid milk, milk powder, yoghurt |
| Cheese | Cheese |
| Soya bean and its products | Tofu, dried bean curd, soy milk |
| Miscellaneous bean | Mung beans, red beans, hemp beans |
| Fats | Lard, butter |
| Vegetable oil | Soybean oil, tea oil, rapeseed oil, olive oil |
| Fast foods | KFC, Mcdonald,fried dough sticks and twists, fried cakes,pizza |
| Nuts | Walnut, peanuts, almonds, melon seeds |
| Snacks | Cookies, sachima, bread, cake, ice cream, candy, sweets,potato chips, shrimp roll, popcorn |
| Chocolates | Chocolates |
| Honey | Honey, hydromel |
| Drinks | Coca-cola, sprite, fruit and vegetable drink, fruits juice |
| Alcoholic beverages | Beer, fruit wine, grape wine |
| Tea | Tea,scented tea, wong Lo Kat |
| Coffee | Coffee |
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Agaricales
Alcoholic Beverages
Almonds
Animals
Apricot
Arachis hypogaea
Bacon
Banana
Bell Pepper
Brachyura
Bread
Cabbage
Candy
Cantaloupes
Cereals
Cheese
Chickens
Chinese
Chocolate
Citrus paradisi
Coffee
Cola
Diet
DNA Chips
Ducks
Eating
Eggs
factor A
Fast Foods
Fats
Fishes
Food
Fowls, Domestic
Fruit
Grapes
Hemp
Honey
Ice Cream
Liver
Meat
Melons
Milk, Cow's
Millets
Nutrients
Nuts
Oil, Rapeseed
Olives
Oryza sativa
Peach
Pears
Plant Tubers
Pork
Potato
Powder
Prunus cerasus
Raphanus
Red Meat
Seafood
Snacks
Sorghum
Soybeans
Spinach
Squid
Strawberries
Tomatoes
Vegetable Oils
Vegetables
Watermelon
Whole Grains
Wine
Hemp hypocotyls were embedded in Technovit 7100 resin (Kulzer). Briefly, sections of 5 mm were fixed in glutaraldehyde/paraformaldehyde/caffeine (1%/2%/1% v/v in Milli-Q water) under vacuum for 15 min and 24 h at 4°C, dehydrated in an ethanol series (70–95–100%), impregnated in resin containing PEG 400 (2% v/v) and dimethacrylate ethylene glycol (0.4% w/v) and finally included. Cross sections of 10 μm thickness were cut using a microtome (Leica) and stained with toluidine blue or used for immunohistochemistry (IHC). Image acquisition was performed with a Leica DMR for toluidine blue and with a confocal microscope LSM 510 Meta (Zeiss) for IHC.
LM5 (β-1,4-galactan), LM10 (xylan) and LM15 (xyloglucan) (Plant Probes) antibodies were diluted 10-fold in milk protein (MP)/PBS (5% w/v). Sections were then incubated for 1.5 h, rinsed three times in PBS and incubated for 1.5 h with the anti-rat IgG coupled to FITC (Sigma) diluted 100-fold in MP/PBS. Before observation, three washing steps with PBS were performed. CBM3a (crystalline cellulose, Plant Probes) was diluted to 10 μg/mL in MP/PBS, incubated in mouse anti-His monoclonal antibody (1% in MP/PBS, Sigma) and finally incubated in 50-fold diluted anti-mouse IgG coupled to FITC (Sigma). Each incubation lasted for 1.5 h. Between each step, three washes with PBS were performed. The slides were mounted in Möwiol 4-88 (Sigma) and observed with the following settings: excitation at 488 nm, filter HFT 488/594 and emission recorded with LP 505. The microscope settings were kept rigorously constant between the different observations for a given epitope. Negative controls where either the primary or secondary antibody was omitted resulted in a very weak and negligible signal.
LM5 (β-1,4-galactan), LM10 (xylan) and LM15 (xyloglucan) (Plant Probes) antibodies were diluted 10-fold in milk protein (MP)/PBS (5% w/v). Sections were then incubated for 1.5 h, rinsed three times in PBS and incubated for 1.5 h with the anti-rat IgG coupled to FITC (Sigma) diluted 100-fold in MP/PBS. Before observation, three washing steps with PBS were performed. CBM3a (crystalline cellulose, Plant Probes) was diluted to 10 μg/mL in MP/PBS, incubated in mouse anti-His monoclonal antibody (1% in MP/PBS, Sigma) and finally incubated in 50-fold diluted anti-mouse IgG coupled to FITC (Sigma). Each incubation lasted for 1.5 h. Between each step, three washes with PBS were performed. The slides were mounted in Möwiol 4-88 (Sigma) and observed with the following settings: excitation at 488 nm, filter HFT 488/594 and emission recorded with LP 505. The microscope settings were kept rigorously constant between the different observations for a given epitope. Negative controls where either the primary or secondary antibody was omitted resulted in a very weak and negligible signal.
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anti-IgG
Antibodies
Caffeine
Cellulose
Debility
Epitopes
Ethanol
Fluorescein-5-isothiocyanate
Galactans
Glutaral
Glycol, Ethylene
Hemp
Hypocotyl
Immunoglobulins
Immunohistochemistry
Microscopy
Microscopy, Confocal
Microtomy
Milk Proteins
Monoclonal Antibodies
Mus
paraform
Plants
polyethylene glycol 400
Resins, Plant
TDO inhibitor LM10
Technovit 7100
Tolonium Chloride
Vacuum
Xylans
xyloglucan
Most recents protocols related to «Hemp»
Example 2
3.5 grams of pine needle essential oil, 20 grams of hydroxypropyl beta cyclodextrin, 3692 grams of Harrell's 8-2-4 liquid fertilizer concentrate liquid fertilizer concentrate, 2.0 grams of humic acid 4.75 grams hemp sap, 80 grams sodium carboxymethylcellulose, 2 drops of color concentrate, and 0.25 grams of nonionic surfactant were combined using a high-speed mixer to produce one gallon of plant treatment concentrate composition. 4 milliliters of the resulting plant treatment concentrate were transferred to a 118 ml. bottle and deionized water was added until filled. A trigger spray top dispenser was added to provide a fragrant foliar nutritional composition for applying to the leaves and stem of an indoor plant.
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2-Hydroxypropyl-beta-cyclodextrin
Elk3 protein, human
Hemp
Humic Acids
Needles
Oils, Volatile
Pinus
Plants
Precipitating Factors
Scents
Sodium Carboxymethylcellulose
Stem, Plant
Surface-Active Agents
Example 8
A portion of the analyzed hemp sap was incorporated into a nutritional composition according to the composition detailed in the following formulation table.
Example 11
In the same manner as used in Example 8, dried barley was reconstituted, and its aqueous extract was juiced and filtered. The elemental analysis is reported in the following table.
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Ammonia
betadex
boric acid
Boron
Boron-8
Boron-9
Boron-10
Boron-12
Boron-13
Calcium, Dietary
calcium nitrate
Calcium Sulfate
Copper
Cyclodextrins
Ethanol
Gluconate, Calcium
Hemp
hempseed oil
Hordeum vulgare
Iron
Lavandula
lavender oil
Magnesium
Manganese
Methoxypectin
Molybdenum
Nitrogen-15
Phosphorus
Plants
Scents
Silicon Dioxide
sodium gluconate
Sulfate, Magnesium
Sulfur
Example 3
3.5 grams of eucalyptus oil, 20 grams of hydroxypropyl beta cyclodextrin, 3772 grams of Harrell's 8-2-4 Formula liquid fertilizer concentrate, 4.75 grams hemp sap, and 0.25 grams of nonionic surfactant, and 30 milliliters of lemon juice are combined with sufficient water, using a high-speed mixer to produce a gallon of plant treatment concentrate. 75 ml. of the resulting concentrate is added to 25 grams of chopped or cut dried hemp and set aside to dry. The resulting fragrant nutrient hemp stock may be used for potted plant mulch or top dressing.
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2-Hydroxypropyl-beta-cyclodextrin
Citrus limon
Eucalyptus Oil
Hemp
Nutrients
Plants
Scents
Surface-Active Agents
Example 7
Freshly harvested industrial hemp plant, including stalks and leaves were fed into a commercial juicer equipped with masticating blade and Archimedes screw feed to separate the fibrous pulp from the sap. The resulting hemp sap was filtered to further remove fibrous solids and thereafter collected as nutritional sap stock. Samples of the hemp sap were sent to a laboratory for elemental analysis. The results of the analysis are reported in Table 2.
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Ammonia
Boron
Boron-8
Boron-9
Boron-10
Boron-12
Boron-13
calcium nitrate
Cannabis
Copper
Dental Pulp
Fibrosis
Hemp
Iron
Manganese
Molybdenum
Nitrogen-15
Phosphorus
Plants
Scents
Silicon Dioxide
Stalking
Sulfur
Dogs received either a placebo or CBD capsule (~4 mg/kg bodyweight) within a Royal Canin® Pill Assist pocket, with their morning meal. Hemp-derived distillate and placebo oils were acquired from Canopy Growth Corporation (Ontario, Canada) and processed by Kazmira LLC (Colorado, USA). The distillate was diluted with a food-grade sunflower oil and manufactured in soft gel capsules (bovine origin; RNA Corporation, Illinois, USA). Capsules were then analyzed for potency and purity as previously described (40 (link)), finding only trace amounts of non-psychoactive cannabidivarin (0.004 mg CBDV/mg of CBD) in a small number of capsules, and no detectible THC or other cannabinoids. The placebo soft gel capsules were manufactured to match the conformity of the CBD-containing capsules, minus the CBD, to maintain the blinding of the study. Two hours after placebo/CBD administration had been confirmed, dogs were exposed to either the separation event or car travel.
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Body Weight
Canis familiaris
cannabidivarin
Cannabinoids
Capsule
Contraceptives, Oral
Food
Hemp
Oil, Sunflower
Oils
Placebos
Reproduction
Top products related to «Hemp»
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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The SUPRA 55VP is a versatile field emission scanning electron microscope (FE-SEM) manufactured by Zeiss. It provides high-resolution imaging and analytical capabilities for a wide range of applications. The SUPRA 55VP is designed to deliver reliable, high-quality results in a compact and user-friendly package.
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The FLUOstar Omega is a multimode microplate reader designed for a variety of fluorescence, luminescence, and absorbance-based applications. It offers high-performance detection capabilities and supports a wide range of microplate formats.
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Acetic acid is a colorless, vinegar-like liquid chemical compound. It is a commonly used laboratory reagent with the molecular formula CH3COOH. Acetic acid serves as a solvent, a pH adjuster, and a reactant in various chemical processes.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
More about "Hemp"
Discover the versatile world of hemp (Cannabis sativa), a remarkable plant with a wide range of applications.
Explore its rich history, diverse uses, and the ongoing research into its therapeutic potential.
Hemp, also known as cannabis or marijuana, has been cultivated for centuries for its strong fibers, nutritious seeds, and potential medicinal compounds.
The plant's fibers can be used to create textiles, paper, and even construction materials, while the seeds are a valuable source of protein, healthy fats, and other beneficial nutrients.
One of the key compounds found in hemp is cannabidiol (CBD), a non-psychoactive cannabinoid that has garnered significant attention for its potential therapeutic benefits.
Researchers are actively exploring the use of CBD and other hemp-derived compounds for managing pain, reducing anxiety, and addressing various inflammatory conditions.
The legal status and regulation of hemp production and use can vary widely across different regions and countries.
In some areas, the cultivation and use of hemp may be subject to strict controls, while in others, it is more readily accessible.
As the understanding of hemp and its diverse applications continues to evolve, researchers and healthcare professionals are working to unlock the full potential of this remarkable plant.
Cutting-edge technologies, such as AI-powered protocol optimization tools like PubCompare.ai, are revolutionizing hemp research by helping scientists locate the best protocols from literature, pre-prints, and patents, ensuring unparalleled reproducibility and accuracy.
Whether you're interested in the industrial applications of hemp, the potential therapeutic benefits of its compounds, or the latest advancements in hemp research, this comprehensive overview provides a glimpse into the fascinating and ever-expanding world of this versatile plant.
Explore its rich history, diverse uses, and the ongoing research into its therapeutic potential.
Hemp, also known as cannabis or marijuana, has been cultivated for centuries for its strong fibers, nutritious seeds, and potential medicinal compounds.
The plant's fibers can be used to create textiles, paper, and even construction materials, while the seeds are a valuable source of protein, healthy fats, and other beneficial nutrients.
One of the key compounds found in hemp is cannabidiol (CBD), a non-psychoactive cannabinoid that has garnered significant attention for its potential therapeutic benefits.
Researchers are actively exploring the use of CBD and other hemp-derived compounds for managing pain, reducing anxiety, and addressing various inflammatory conditions.
The legal status and regulation of hemp production and use can vary widely across different regions and countries.
In some areas, the cultivation and use of hemp may be subject to strict controls, while in others, it is more readily accessible.
As the understanding of hemp and its diverse applications continues to evolve, researchers and healthcare professionals are working to unlock the full potential of this remarkable plant.
Cutting-edge technologies, such as AI-powered protocol optimization tools like PubCompare.ai, are revolutionizing hemp research by helping scientists locate the best protocols from literature, pre-prints, and patents, ensuring unparalleled reproducibility and accuracy.
Whether you're interested in the industrial applications of hemp, the potential therapeutic benefits of its compounds, or the latest advancements in hemp research, this comprehensive overview provides a glimpse into the fascinating and ever-expanding world of this versatile plant.