The largest database of trusted experimental protocols
> Chemicals & Drugs > Organic Chemical > Heparin Sodium

Heparin Sodium

Heparin Sodium: A naturally-occurring anticoagulant used to prevent and treat thrombosis.
It acts by enhancing the inhibitory effect of antithrombin III on several activated clotting factors, including factors IIa, IXa, Xa, and XIIa.
Heparin Sodium is derived from mucosal tissues, typically bovine or porcine, and is available in various pharmaceutical formulations for clinical use.
It plays a crucial role in cardiovascular and hematologic therapies, with applications in the managment of conditions like deep vein thrombosis, pulmonary emoblism, and post-operative anticoagulation.
Reseachers can use PubCompare.ai's AI-powered platform to easily locate Heparin Sodium protocaols from literature, preprints, and patents, and identify the best protocols and products to streamline their research process.

Most cited protocols related to «Heparin Sodium»

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2013
artenimol Atmosphere Biological Assay BLOOD Cell Nucleus Erythrocytes Gentamicin Heparin Sodium Parasitemia Parasites Percoll Pharmaceutical Preparations Schizonts Sorbitol Sulfoxide, Dimethyl Thermal Plasma Trophozoite Volumes, Packed Erythrocyte
135 COVID-19 patients admitted to Yale-New Haven Hospital (YNHH) between March 18th and May 5th, 2020 were included in this study. Nasopharyngeal swabs were collected, as recently described23 (link), approximately every four days for SARS-CoV-2 RT-qPCR analysis where clinically feasible. Paired whole blood for flow cytometry analysis was collected simultaneously in sodium heparin-coated vacutainers and kept on gentle agitation until processing. All blood was processed the same day as collection from patients. Patients were scored for COVID-19 disease severity through review of electronic medical records (EMR) at each longitudinal time point. Scores were assigned by a clinical infectious disease physician according to a custom developed disease severity scale. Moderate disease status (Clinical Score 1, 2 and 3) was defined as: (1) SARS-CoV-2 infection requiring hospitalization without supplemental oxygen, (2) infection requiring non-invasive supplemental oxygen (<3 L / min, sufficient to maintain greater than 92% SpO2), (3) infection requiring non-invasive supplemental oxygen (> 3L supplemental oxygen to maintain SpO2 > 92%, or, required > 2L supplemental oxygen to maintain SpO2 > 92% and had a high sensitivity C-reactive protein (CRP) > 70) and received tocilizumab. Severe disease status (Clinical score 4 and 5) was defined as infection meeting all criteria for clinical score 3 while also requiring admission to the YNHH Intensive Care Unit (ICU) and > 6L supplemental oxygen to maintain SpO2 > 92% (4); or infection requiring invasive mechanical ventilation / extracorporeal membrane oxygenation (ECMO) in addition to glucocorticoid / vasopressor administration (5). Clinical score 6 was assigned for deceased patients. Of note, the use of tocilizumab can increase circulating levels of IL-6 through inhibition of IL-6Rα-mediated degradation. Analysis of our cohort indicate higher plasma levels of IL-6 in both moderate and severe patients that received tocilizumab treatment (Extended data Fig. 1d).
For all patients, days from symptom onset were estimated according to the following scheme: (1) highest priority was given explicit onset dates provided by patients; (2) next highest priority was given to the earliest reported symptom by a patient, and (3) in the absence of direct information regarding symptom onset, we estimated a date through manual assessment of the electronic medical record (EMRs) by an independent clinician. Demographic information was aggregated through a systematic and retrospective review of patient EMRs and was used to construct Extended Table 1. Symptom onset and etiology was recorded through standardized interview with patients or patient surrogates upon enrollment in our study, or alternatively through manual EMR review if no interview was possible due to clinical status. The clinical data was collected using EPIC EHR and REDCap 9.3.6 software.
Publication 2020
BLOOD Communicable Diseases COVID 19 C Reactive Protein Extracorporeal Membrane Oxygenation Flow Cytometry Glucocorticoids Hematologic Tests Heparin Sodium Hospitalization Infection Mechanical Ventilation Nasopharynx Oxygen Patients Physicians Plasma Psychological Inhibition SARS-CoV-2 Saturation of Peripheral Oxygen tocilizumab Vasoconstrictor Agents
135 COVID-19 patients admitted to Yale-New Haven Hospital (YNHH) between March 18th and May 5th, 2020 were included in this study. Nasopharyngeal swabs were collected, as recently described23 (link), approximately every four days for SARS-CoV-2 RT-qPCR analysis where clinically feasible. Paired whole blood for flow cytometry analysis was collected simultaneously in sodium heparin-coated vacutainers and kept on gentle agitation until processing. All blood was processed the same day as collection from patients. Patients were scored for COVID-19 disease severity through review of electronic medical records (EMR) at each longitudinal time point. Scores were assigned by a clinical infectious disease physician according to a custom developed disease severity scale. Moderate disease status (Clinical Score 1, 2 and 3) was defined as: (1) SARS-CoV-2 infection requiring hospitalization without supplemental oxygen, (2) infection requiring non-invasive supplemental oxygen (<3 L / min, sufficient to maintain greater than 92% SpO2), (3) infection requiring non-invasive supplemental oxygen (> 3L supplemental oxygen to maintain SpO2 > 92%, or, required > 2L supplemental oxygen to maintain SpO2 > 92% and had a high sensitivity C-reactive protein (CRP) > 70) and received tocilizumab. Severe disease status (Clinical score 4 and 5) was defined as infection meeting all criteria for clinical score 3 while also requiring admission to the YNHH Intensive Care Unit (ICU) and > 6L supplemental oxygen to maintain SpO2 > 92% (4); or infection requiring invasive mechanical ventilation / extracorporeal membrane oxygenation (ECMO) in addition to glucocorticoid / vasopressor administration (5). Clinical score 6 was assigned for deceased patients. Of note, the use of tocilizumab can increase circulating levels of IL-6 through inhibition of IL-6Rα-mediated degradation. Analysis of our cohort indicate higher plasma levels of IL-6 in both moderate and severe patients that received tocilizumab treatment (Extended data Fig. 1d).
For all patients, days from symptom onset were estimated according to the following scheme: (1) highest priority was given explicit onset dates provided by patients; (2) next highest priority was given to the earliest reported symptom by a patient, and (3) in the absence of direct information regarding symptom onset, we estimated a date through manual assessment of the electronic medical record (EMRs) by an independent clinician. Demographic information was aggregated through a systematic and retrospective review of patient EMRs and was used to construct Extended Table 1. Symptom onset and etiology was recorded through standardized interview with patients or patient surrogates upon enrollment in our study, or alternatively through manual EMR review if no interview was possible due to clinical status. The clinical data was collected using EPIC EHR and REDCap 9.3.6 software.
Publication 2020
BLOOD Communicable Diseases COVID 19 C Reactive Protein Extracorporeal Membrane Oxygenation Flow Cytometry Glucocorticoids Hematologic Tests Heparin Sodium Hospitalization Infection Mechanical Ventilation Nasopharynx Oxygen Patients Physicians Plasma Psychological Inhibition SARS-CoV-2 Saturation of Peripheral Oxygen tocilizumab Vasoconstrictor Agents
Blood samples were collected from 4 healthy volunteers using the following blood collection tubes; normal clotting tube (SST II Advance, BD Biosciences) for serum and sodium heparin (NH), EDTA, or sodium citrate (NC) tubes for collecting plasma (all BD Biosciences) in the morning on 2 following days. All samples were kept on room temperature and were spun within 1 hour at 700 × g at room temperature. Cell free plasma or serum was aliquoted and stored at -80°C until analysis. Before analysis all thawed samples were centrifuged through a polypropylene centrifuge tube containing a 0.22 μm nylon membrane (Spin-X column; Corning, Corning, NY, USA) to remove debris. Non-specific heterophilic antibodies, such as natural polyspecfic antibodies, idiotypic antibodies and natural rheumatoid factors, were pre-absorbed, without loss of cytokine concentration, from all samples with protein-L pre coated ELISA plates (Pierce, Rockford, IL, USA) as described previously [14 (link)]. 100 μl of sample well was incubated for 1 hour at room temperature under continuous shaking. As this incubation step removes 60-80% of the total immunoglobulin fraction without depleting natural occurring cytokines [14 (link)]samples were then diluted with 10% v/v normal rat and mouse serum (1:1 ratio; Rockland, Gilbertsville PA, USA) and incubated for 10 additional minutes at room temperature to block any residual interfering proteins which are able to bind (a) specifically to rat or mouse immunoglobulins. Animal serum batches were tested upfront, and only used when they did not show any cross-reactivity within the assay.
All samples obtained were approved for collection by the medical ethical committee of the UMC Utrecht. Informed consent was obtained from each individual who donated samples.
Full text: Click here
Publication 2009
Animals Antibodies Biological Assay BLOOD Cardiac Arrest Cross Reactions Cytokine Edetic Acid Enzyme-Linked Immunosorbent Assay Healthy Volunteers Heparin Sodium Heterophile Antibodies Immunoglobulins Mus Nylons Plasma Plasma Cells Polypropylenes Proteins Rheumatoid Factor Serum Sodium Citrate Tissue, Membrane
A healthy cannabis smoker provided written informed consent to participate in a study investigating cannabinoid pharmacokinetics, in vitro cannabinoid stability and novel markers of cannabis intake following a single smoked cannabis dose. The Institutional Review Board of the National Institute on Drug Abuse, National Institutes of Health approved this protocol. Cannabis cigarettes contained 6.8% THC (w/w) or approximately 56 mg THC and were smoked ad libitum over a 10 min period following an overnight stay on a secure residential unit. Whole blood was collected with sodium heparin 0.5 h prior to, 0.25 h after and 1.0 h after the start of cannabis smoking. Blood was transferred to polypropylene storage tubes and stored refrigerated until analysis within 24 h.
Publication 2011
BLOOD Cannabinoids Cannabis Drug Kinetics Ethics Committees, Research Heparin Sodium Polypropylenes

Most recents protocols related to «Heparin Sodium»

Fasting blood samples were collected between 9:30 and 11:30 a.m. into sodium heparin blood collection tubes. Histopaque®-1077 (2.5 ml) was added to the bottom of the tube containing 5 ml blood and centrifuged for 20 min at 610g at 4 °C without the brake. Tubes were visually inspected for proper separation and hemolysis. The top layer containing plasma was aliquoted and stored at − 80 °C.
Gelsolin was measured by BioAegis® Therapeutics by a proprietary ELISA using a primary antibody raised against the N terminal tail specific to plasma gelsolin [8 (link)]. Samples from the different visits were run on the same plate for each individual, with the exception of nine samples. Plates were balanced to contain individuals across race and diabetes diagnosis across all plates. Each sample was run in triplicate, and the sample average was used. The coefficient of variance (CV) for sample replicates was 3.73% (time 1) and 3.49% (time 2). Controls (QC) were produced by spiking recombinant human plasma gelsolin into a plasma sample at three different concentrations 10 ng/ml, 50 ng/ml and 100 ng/ml; the same donor plasma was used for the entire study. In addition, the 50 ng/ml spike-in control (QC-M) was aliquoted, stored at − 80 °C and run on each subsequent plate. The mean intra-assay CV was 3.64% and 3.99% for QC and QC-M respectively. The mean inter-assay CV was 6.05% and 6.19%, respectively.
Full text: Click here
Publication 2023
Biological Assay BLOOD Diabetes Mellitus Diagnosis Enzyme-Linked Immunosorbent Assay Gelsolin Hemolysis Heparin Sodium histopaque Homo sapiens Immunoglobulins Plasma Tail Therapeutics Tissue Donors

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Activated Partial Thromboplastin Time Antithrombin III BLOOD Citrates C Reactive Protein Creatinine Diagnosis Edetic Acid Factor VIII Factor VIII-Related Antigen fibrin fragment D Fibrinogen Heparin Sodium Patient Admission Patients Protein C protein S, human Serum
Blood sampling was performed as previously described (25 ). After euthanasia with 0.02% MS222, blood was collected from the caudal vein using a glass needle (outer diameter 1 mm, inner diameter 0.5 mm; GD-1; Narishige) coated with 0.05 U/µL heparin sodium (Sigma) in phosphate-buffered saline (PBS). The blood was stored at −80 °C until use. For transcript level analysis, pituitaries were isolated and directly transferred into tubes containing 300 µL of TRIzol (Invitrogen) and 6 zirconium oxide beads (diameter 1.4 μm; Bertin Technologies). For cell imaging analysis, the brain–pituitary complexes were fixed overnight at 4 °C in 4% paraformaldehyde (Electron Microscopy 135 Sciences) in phosphate-buffered saline with Tween (PBST: PBS, 0.1%; Tween-20). After washing with PBST, tissues were serially dehydrated in 25, 50, 75, and 96% ethanol at room temperature for 10 minutes each, followed by storage in 100% methanol at −20 °C until use.
Publication 2023
BLOOD Brain Cells Electron Microscopy Ethanol Euthanasia Heparin Sodium Methanol Needles paraform Phosphates Saline Solution Tissues trizol Tween 20 Tweens Veins zirconium oxide

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Anticoagulants BLOOD Cells Erythrocytes Fluorescein-5-isothiocyanate Heparin Sodium Muromonab-CD3 paraform Sodium Chloride
Extremely premature-born animals were cared for in humidified, heated incubators (Drägerwerk) as outlined in Fig. 1A. In brief, PC-CMV was continuously adapted to maintain paO2 between 7.3 and 9.3 kPa (55-70 mmHg) and paCO2 between 6.0 and 7.3 kPa (45-55 mmHg). Ketamine and midazolam (Akorn) were provided as required. Heparinized, sodium- and pH-adapted maintenance fluids (normal saline, half-normal saline, or sodium acetate with 1 U/mL unfractionated heparin—all from Hospira) and an individually prepared, amino acid-rich, electrolyte, and glucose-adapted total parenteral nutrition were infused alongside intravenous lipids. Maintenance fluid rates were adapted to maintain mean arterial pressures> >25 mmHg. If hypotensive, a maximum of 2 boluses (10 mL/kg) normal saline was given and escalated to inotropic therapy if required with dopamine ± dobutamine (both from Hospira) up to doses of 20 µg/kg/min, respectively. If hypotension persisted, hydrocortisone at 1 mg/kg every 12 hours was initiated and tapered as soon as possible. Acidosis was buffered with sodium bicarbonate (Hospira) at 1 mmol/kg. Animals were transfused with stored placental or maternal packed blood cells at 10 mL/kg if hematocrits persisted below 30%. To prevent sepsis, preemptive ampicillin (50 mg/kg), gentamicin (2.5 mg/kg) and vancomycin (15 mg/kg, all from Hospira) were given (Fig. 1A). Whole-body anteroposterior X-ray films were taken every 24 hours to assess the lung, heart, intestines and evaluate the position of the endotracheal tube and the intravascular catheters. Echocardiographic studies were performed daily by a pediatric cardiologist; an open ductus arteriosus was neither treated medically, nor surgically.
Publication 2023
Acetate Acidosis Amino Acids Ampicillin Animals Bicarbonate, Sodium BLOOD Cardiologists Catheters Childbirth Dobutamine Dopamine Ductus Arteriosus Echocardiography Electrolytes Gentamicin Glucose Heart Heparin Sodium Human Body Hydrocortisone Intestines Ketamine Lipids Lung Midazolam Normal Saline Operative Surgical Procedures Parenteral Nutrition, Total Placenta Premature Birth Septicemia Sodium Stem Cells Therapeutics Vancomycin Volumes, Packed Erythrocyte X-Ray Film

Top products related to «Heparin Sodium»

Sourced in United States, United Kingdom, Italy, Germany, China
Heparin sodium is an anticoagulant medication used to prevent and treat blood clots. It is a natural substance derived from porcine (pig) intestinal mucosa. Heparin sodium acts by enhancing the activity of antithrombin, a naturally occurring substance that inactivates certain clotting factors, thereby reducing the risk of thrombus formation.
Sourced in United States, United Kingdom, Brazil, Germany, Canada, France, Spain, Italy, Switzerland, Australia, Sweden, Belgium, New Zealand, Denmark, Mexico, Jersey, South Sudan, Austria, Japan
The BD Vacutainer is a blood collection system used to collect, process, and preserve blood samples. It consists of a sterile evacuated glass or plastic tube with a closure that maintains the vacuum. The Vacutainer provides a standardized method for drawing blood samples for laboratory analysis.
Sourced in United States, Germany, United Kingdom, Switzerland
Heparin sodium salt is a natural anticoagulant compound used in various laboratory applications. It functions as an anticoagulant by inhibiting the activity of thrombin and other proteases involved in the blood clotting process. Heparin sodium salt is widely used in research settings to prevent coagulation of blood samples, enabling accurate analysis and testing.
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
Sourced in United States, Germany
Sodium heparin is a type of anticoagulant used in laboratory settings. It is a salt of heparin, a naturally occurring substance that helps prevent blood clotting. Sodium heparin is commonly used in blood collection tubes to prevent coagulation of the sample, allowing for accurate analysis of blood components.
Sourced in Sweden, United States, United Kingdom, Germany, Canada, Japan, Italy, China, France, Australia, Belgium, Austria, Spain, Switzerland, Norway, New Zealand, Denmark, Finland, Georgia
Ficoll-Paque PLUS is a sterile, ready-to-use medium for the isolation of mononuclear cells from blood or bone marrow by density gradient centrifugation. It is a polysucrose and sodium diatrizoate solution with a density of 1.077 g/mL.
Sourced in United States, United Kingdom, Canada, Australia, Germany, France, Brazil, Spain, Switzerland, Sweden, Belgium, Singapore, New Zealand, Italy, Japan, India
Vacutainer tubes are laboratory collection tubes used to obtain blood samples from patients. They are designed to maintain the integrity of the collected sample and prevent contamination. The tubes come in various sizes and contain different additives that help preserve the sample for subsequent analysis.
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
Sourced in United States, United Kingdom
Sodium heparin tubes are laboratory sample collection tubes used to collect blood samples. They contain sodium heparin, an anticoagulant that prevents the blood from clotting during the collection and transportation process.
Sourced in United States, France
Sodium heparin Vacutainers are laboratory blood collection tubes used to collect and store blood samples. They contain the anticoagulant sodium heparin to prevent the blood from clotting during the collection and transportation process.

More about "Heparin Sodium"

Heparin Sodium is a naturally-occurring anticoagulant used to prevent and treat thrombosis, acting by enhancing the inhibitory effect of antithrombin III on several activated clotting factors.
Derived from mucosal tissues, typically bovine or porcine, it is available in various pharmaceutical formulations for clinical use.
Heparin plays a crucial role in cardiovascular and hematologic therapies, with applications in the management of conditions like deep vein thrombosis, pulmonary embolism, and post-operative anticoagulation.
Researchers can utilize PubCompare.ai's AI-powered platform to easily locate Heparin Sodium protocols from literature, preprints, and patents, and identify the best protocols and products to streamline their research process.
This includes accessing information on related terms like BD Vacutainer, Heparin sodium salt, FBS (fetal bovine serum), Sodium heparin, Ficoll-Paque PLUS, Vacutainer tubes, DMSO, Sodium heparin tubes, and Sodium heparin Vacutainers.
By leveraging these insights, researchers can make more informed decisions and optimize their work with Heparin Sodium.