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Hexafluoroisopropanol

Hexafluoroisopropanol is a unique chemical compound with diverse applications in research and industry.
This fluorinated alcohol possesses interesting physicochemical properties, making it a valuable tool for scientists and researchers.
PubCompare.ai's AI-driven platform can help optimize your research protocols and enhance reproducibility by providing easy access to protocols from literature, preprints, and patents, and leveraging AI-driven comparisons to identify the best protocols and products for your needs.
Streamline your research process and discover the power of Hexafluoroisopropanol with PubCompare.ai's powerful tools.ode

Most cited protocols related to «Hexafluoroisopropanol»

Fabrication of PLGA Nanofiber Mats

Cited 23 times

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Publication 2015

Aluminum Fibrosis Gamma Rays glycolic acid hexafluoroisopropanol Molar Needles Poly A Polylactic Acid-Polyglycolic Acid Copolymer Steel Viscosity

Metabolite Extraction and Analysis Protocol

Cited 16 times

Cells were washed twice with phosphate buffered saline (Mediatech, Manassas, VA) (1 mM pH 7.4) before being scraped into 750 µL of ice-cold methanol/water (4/1 v/v). For cells treated with rapamycin, samples were spiked with internal standards (500 ng [¹³C₄]-succinate, 500 ng [¹³C₆]-citrate, 500 ng [¹³C₃]-pyruvate, 2 µg [¹³C₃]-lactate, 500 ng [¹³C₄,¹⁵N]-aspartate, 2 µg [¹³C₅,¹⁵N]-glutamate and 500 ng [¹³C₆]-glucose 6-phosphate). Samples were pulse-sonicated for 30 s with a probe tip sonicator and centrifuged at 16,000 × g for 10 min. The supernatant was transferred to two new tubes: 50 µL were transferred to one tube and diluted 5 times with 50 mM ammonium carbonate for direct analysis of the underivatized redox cycling metabolites (Figure 2) and 700 µL were transferred to one tube containing 300 µL of phenylhydrazine in water (3 mg/mL) for analysis of underivatized and derivatized metabolites (Figure 2). Derivatization was conducted by incubation at room temperature for 2 h before evaporation to dryness under nitrogen. 100 µL of water was used to re-suspend the samples. Injection volume was 5 µL in both methods. The phenylhydrazine-derivatized samples were run with gradient 1 and the underivatized samples were run with gradient 2.

Guo L., Worth A.J., Mesaros C., Snyder N.W., Glickson J.D, & Blair I.A. (2016). Diisopropylethylamine/hexafluoroisopropanol-mediated ion-pairing UHPLC-MS for phosphate and carboxylate metabolite analysis: utility for studying cellular metabolism. Rapid communications in mass spectrometry : RCM, 30(16), 1835-1845.

Publication 2016

ammonium carbonate Aspartate Cells Citrates Cold Temperature Glucose-6-Phosphate Glutamates Ice Lactates Methanol Nitrogen Oxidation-Reduction phenylhydrazine Phosphates Pulse Rate Pyruvate Saline Solution Sirolimus Succinate

Preparation of Monomeric and Oligomeric Amyloid-Beta

Cited 14 times

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Publication 2010

Bath Cold Temperature hexafluoroisopropanol Homo sapiens Homozygote Peptides polypeptide C Sulfoxide, Dimethyl Syringes

Protocol for Analyzing Neuronal-Glial Cell Stress

Cited 14 times

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Publication 2011

Actins ALOX15 protein, human Antioxidants beta-Tubulin Biological Assay Brain Brain Diseases Caimans Cell Culture Techniques Cells Chelating Agents Complement System Proteins Electrophoretic Mobility Shift Assay Faculty Gels Glial Fibrillary Acidic Protein guanidine isothiocyanate hexafluoroisopropanol Homo sapiens I-kappa B Proteins Immunoglobulins Interleukin-1 beta isolation MicroRNAs Microtubule-Associated Proteins MIRN9 microRNA, human neuro-oncological ventral antigen 2, human Neuroglia Neurons NF-kappa B Northern Blot Peptides Phosphotransferases Primary Cell Culture prolinedithiocarbamate Proteins pyrrolidine dithiocarbamic acid Quantitative Real-Time Polymerase Chain Reaction Rabbits Reproduction Reverse Transcription RNA, Messenger Serum Albumin, Human Silica Gel Sulfoxide, Dimethyl Tissue, Membrane Translocation, Chromosomal

IAPP Monomerization and NMR Sample Prep

Cited 11 times

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Publication 2011

Amylin Buffers cysteinylcysteine Disulfides hexafluoroisopropanol Homo sapiens Isotopes Peptides Phosphates Sodium-20 Sodium Chloride sodium phosphate Solvents

Most recents protocols related to «Hexafluoroisopropanol»

Oligonucleotide Structural Analysis by LC-MS

Not available on PMC !

The structure of the solid-phase synthesized oligonucleotides was determined by liquid chromatographymass spectrometry (LC-MS) using an Agilent 6120 series single quadrupole LC/MS system (Agilent Technologies). Liquid chromatography was performed using an ACQUITY BEH C18 column (1.7 mm, 2.1 • 50 mm; Waters) with buffers A (8.6 mM trimethylamine and 100 mM hexafluoroisopropanol in water) and B (methanol).

Iwai H., Kimura Y., Honma M., Nakamoto K., Motosawa K., Atago T., Asano K., Hashiya F., Abe N., Kobayashi K., Ogisu R., Hashimoto A., Hiraishi K., Saito S., Yamamoto J., & Abe H. (2024). Position-Specific Nucleoside Sugar Modifications in mRNA ORF: Enhancing Translational Function through Complete Chemical Synthesis of mRNA.

Publication 2024

In Vitro Cell Culture Protocol

PLGA (lactide/glycolide ratio of 50:50, M_W = 100,000 Da), PCL (M_W = 80,000 Da), and 1,1,1,3,3,3-Hexafluoroisopropanol (HFIP) solution were purchased from Aladdin (Shanghai, China). Phosphate-buffered saline (PBS), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin (P/S) were bought from (Shanghai, China). Cell counting-kit-8 (CCK8) reagent was acquired from APExBIO (Beijing, China). Live/dead staining reagents were obtained from Solarbio (Shanghai, China).

Qiao H., Gao C., Lu C., Liu H., Zhang Y., Jin A., Dai Q., Yang S., Zhang B, & Liu Y. (2024). A Novel Method for Fabricating the Undulating Structures at Dermal—Epidermal Junction by Composite Molding Process. Journal of Functional Biomaterials, 15(4), 102.

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Publication 2024

Vancomycin-Loaded Chitosan-PLGA Nanoparticles

Vancomycin HCl was purchased from Tocris
Bioscience (Minneapolis). Chitosan medium molecular weight (Mw 47,000
Da), poly(vinyl alcohol) (PVA; Mw 146,000–186,000 Da), poly(ethylene
oxide) (PEO; Mw 600,000), and ammonium dihydrogen phosphate were purchased
from Sigma-Aldrich (Burlington). Glacial acetic acid high-performance
liquid chromatography (HPLC) grade, dimethylformamide (DMF), hexafluoroisopropanol
(HFIP), dichloromethane (DCM), and acetone were purchased from Thermo
Fisher Scientific (Waltham). Poly(lactic-co-glycolic
acid) (PLGA) 50:50 was purchased from Durect (Cupertino).

Serpelloni S., Williams M.E., Caserta S., Sharma S., Rahimi M, & Taraballi F. (2024). Electrospun Chitosan-Based Nanofibrous Coating for the Local and Sustained Release of Vancomycin. ACS Omega, 9(10), 11701-11717.

Publication 2024

Peptide Solubilization and Aggregation

Peptides were dissolved at 2.5 mM in hexafluoroisopropanol (HFIP). The peptide solution was then added to sterile water at a 1:9 ratio to yield a final solution containing 250 µM peptide in 10% HFIP. Solutions were then either annealed (heated to 95 °C for 30 min and cooled to 25 °C at 1 °C/min) or aged 2 h at room temperature. Assemblies were stored at 4 °C.

Klawa S.J., Lee M., Riker K.D., Jian T., Wang Q., Gao Y., Daly M.L., Bhonge S., Childers W.S., Omosun T.O., Mehta A.K., Lynn D.G, & Freeman R. (2024). Uncovering supramolecular chirality codes for the design of tunable biomaterials. Nature Communications, 15, 788.

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Publication 2024

Synthesis of Bioactive Composite Materials

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Publication 2024

Top products related to «Hexafluoroisopropanol»

Hexafluoroisopropanol by Merck Group

Sourced in United States

Hexafluoroisopropanol is a highly fluorinated organic compound used as a specialty chemical in various industrial and laboratory applications. It is a colorless, volatile liquid with a distinctive odor. Hexafluoroisopropanol is known for its unique physical and chemical properties, which make it a useful solvent and reagent in specific research and manufacturing processes.

Dimethyl sulfoxide (dmso) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh

DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

Hexafluoroisopropanol hfip by Merck Group

Sourced in United States

Hexafluoroisopropanol (HFIP) is a specialty chemical compound used primarily as a solvent and reagent in various laboratory applications. It has a unique chemical structure with a high degree of fluorination, which gives it distinct physical and chemical properties. HFIP is known for its ability to dissolve a wide range of organic compounds, making it a versatile tool in chemical synthesis and analysis.

Advancebio oligonucleotides c18 by Agilent Technologies

AdvanceBio oligonucleotides C18 are a type of analytical column used in high-performance liquid chromatography (HPLC) for the separation and purification of oligonucleotides. The column features a C18 stationary phase designed for efficient and reliable oligonucleotide analysis.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Penicillin streptomycin by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia

Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

1 1 1 3 3 3 hexafluoroisopropanol hfip by Merck Group

Sourced in United States

1,1,1,3,3,3-hexafluoroisopropanol (HFIP) is a fluorinated organic compound with the chemical formula (CF3)2CHOH. It is a colorless, dense liquid with a high boiling point. HFIP is commonly used as a solvent in various laboratory applications due to its unique chemical properties.

Aβ1 42 by AnaSpec

Sourced in United States, Belgium

Aβ1–42 is a synthetic peptide that corresponds to the first 42 amino acids of the amyloid-beta (Aβ) protein. It is commonly used in research and development for the study of Alzheimer's disease and other neurodegenerative disorders.

Methanol by Merck Group

Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan

Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.

6530 accurate mass q tof by Agilent Technologies

Sourced in United States, Germany

The 6530 Accurate-Mass Q-TOF is a quadrupole time-of-flight (Q-TOF) mass spectrometer designed for high-resolution, accurate-mass analysis. It features a dual-spray ionization source and provides precise mass measurements for a wide range of applications.

What are the unique properties of Hexafluoroisopropanol that make it a valuable tool for researchers?

Hexafluoroisopropanol is a fluorinated alcohol with interesting physicochemical properties, such as high acidity, polarity, and ability to form strong hydrogen bonds. These unique characteristics make it a versatile solvent and reagent in various research applications, including organic synthesis, polymer chemistry, and biochemical studies. The fluorinated nature of Hexafluoroisopropanol also contributes to its distinct solvation behavior and ability to interact with biomolecules in unique ways.

How can PubCompare.ai help researchers optimize the use of Hexafluoroisopropanol in their protocols?

PubCompare.ai's AI-driven platform can assist researchers in streamlining their use of Hexafluoroisopropanol by providing easy access to protocols from literature, preprints, and patents. The platform's AI-driven comparisons can help identify the most effective protocols and products related to Hexafluoroisopropanol for specific research goals. This enables researchers to choose the best option for reproducibility and accuracy, enhancing the overall efficiency and quality of their work.

What are some common challenges researchers face when working with Hexafluoroisopropanol, and how can PubCompare.ai help address them?

One common challenge with Hexafluoroisopropanol is its high acidity and reactivity, which can sometimes lead to compatibility issues with certain materials or experimental setups. PubCompare.ai's platform can help researchers identify protocols that addess these challenges, such as by providing information on suitable containment materials or optimal reaction conditions. Additionally, the platform's AI-driven analysis can highlight key differences in protocol effectiveness, enabling researchers to choose the most robust and reliable methods for their work with Hexafluoroisopropanol.

Are there different variations or types of Hexafluoroisopropanol, and how do they differ in their applications?

Yes, there are several variations of Hexafluoroisopropanol, including anhydrous and aqueous forms, as well as different purits levels. The anhydros form is commonly used in organic synthesis and polymer chemistry, while the aqueous form finds applications in biochemical research, such as protein purification and stabilization. The purity level of Hexafluoroisopropanol can also be important, depending on the specific application, and PubCompare.ai can help researchers identify the most suitable form and purity for their needs.

How can PubCompare.ai assist researchers in discovering new applications for Hexafluoroisopropanol?

PubCompare.ai's AI-driven platform can help researchers discover new and emerging applications for Hexafluoroisopropanol by providing a comprehensive view of the existing literature and protocols. The platform's search functionality and data visualizations can enable researchers to quickly identify trends, patterns, and novel use cases for this versatiel chemical. Additionally, the AI-driven comparisons can highlight unexpected synergies or combinations of Hexafluoroisopropanol with other reagents or techniques, potentially leading to the discovery of novel applications. By streamlining the research process and leveraging AI-powered insights, PubCompare.ai can be a powerful tool for expanding the horizons of Hexafluoroisopropanol utilization.

More about "Hexafluoroisopropanol"

Hexafluoroisopropanol (HFIP) is a unique and versatile fluorinated alcohol with a wide range of applications in research and industry.
This compound, also known as 1,1,1,3,3,3-hexafluoroisopropanol, possesses intriguing physicochemical properties that make it a valuable tool for scientists and researchers.
HFIP is commonly used as a solvent for proteins, peptides, and other biomolecules, including Aβ1–42, due to its ability to disrupt hydrogen bonding and solubilize these compounds.
This property makes it useful in the study of protein structure and aggregation, as well as in the preparation of oligonucleotides like AdvanceBio oligonucleotides C18.
In addition to its solvent capabilities, HFIP has been employed in the synthesis of fluorinated polymers and as a component in chemical reactions.
It has also found applications in the analysis of complex samples, such as those encountered in mass spectrometry, where it can be used in conjunction with techniques like 6530 Accurate-Mass Q-TOF.
To optimize your research protocols and enhance reproducibility, PubCompare.ai's AI-driven platform can provide easy access to a wealth of information on HFIP and related compounds, such as DMSO and methanol.
The platform's powerful tools enable you to compare protocols from literature, preprints, and patents, helping you identify the best approaches and products for your specific needs.
By leveraging the insights and capabilities of PubCompare.ai, you can streamline your research process, discover new applications for HFIP, and unlock the full potential of this versatile chemical compound.
Embrace the power of HFIP and let PubCompare.ai guide you towards more efficient and reproducible research.