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Hoechst33342

Hoechst 33342 is a fluorescent dye that binds to the minor groove of DNA, emitting blue fluorescence when excited.
It is commonly used in cell biology research for staining nuclei, tracking cell division, and identifying apoptotic cells.
The PubCompare.ai platform helps researchers discover the optimal Hoechst33342 protocols by leveraging data-driven comparisons of scientific literature, preprints, and patents to find the most up-to-date and effective solutions for their research needs.
This AI-driven platform enables users to locate and compare Hoechts33342 protocols, ensuring they have access to the best methods for their specific experimental requirements.

Most cited protocols related to «Hoechst33342»

1
Comprehensive Resource of Natural Compounds
Cited 20 times
A chemical library of 658-natural compounds was kindly provided by Dr. Sang Jeon Chung of Sungkyunkwan University (Suwon, Korea). Kaempferide (69545), dimethylsulfoxide (D2650), bafilomycin A1 (B1793), rapamycin (553210), tiliroside (79257), chloroquine (C6628), orlistat (O4139), palmitic acid (P5585), oleic acid (O1383), acridine orange (A6014), oil-red-O (O0625), dexamethasone (D8893), insulin (I0516), and 3-isobutyl-1-methylxanthine (I5879) were purchased from Sigma-Aldrich. BODIPY 493/503 (D3922), Hoechst33342 (H3570), lipofectamine LTX (94756), lipofectamine 2000 (52887), Plus reagent (10964), protease and phosphatase inhibitor solution (78441), M-PER kit (89842Y), DMEM, fetal bovine serum (FBS), bovine serum, and antibiotics were purchased from Invitrogen ThermoFisher Scientific. For in vivo experiments, Kaempferide (K0057) was purchased from TCI Chemicals. siRNA targeting TUFM was purchased from Dharmacon. mRFP-GFP-LC3B plasmids were kindly provided by Dr. Jaewhan Song of Yonsei University (Seoul, Korea).
Kim D., Hwang H.Y., Ji E.S., Kim J.Y., Yoo J.S, & Kwon H.J. (2021). Activation of mitochondrial TUFM ameliorates metabolic dysregulation through coordinating autophagy induction. Communications Biology, 4, 1.
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Publication 2021
1-Methyl-3-isobutylxanthine 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene Acridine Orange Antibiotics, Antitubercular bafilomycin A1 Bos taurus Chloroquine Dexamethasone Fetal Bovine Serum Hoechst33342 Insulin kaempferide Lipofectamine lipofectamine 2000 Oleic Acid Orlistat Palmitic Acid Peptide Hydrolases Phosphoric Monoester Hydrolases Plasmids RNA, Small Interfering Serum Sirolimus solvent red 27 Sulfoxide, Dimethyl tiliroside
2
Live Cell Imaging and Immunofluorescence Analysis
Cited 7 times
For live cell imaging, cells were cultured in CO2 independent media (Invitrogen) with 50-100 ng/ml Hoechst33342 for 30 min prior to observation. Immunofluorescence in human cells was conducted as described previously22 (link) using antibodies against fibronectin (a generous gift from Richard Hynes, 1:10,000), tubulin (DM1α; Sigma, 1:500), p150 dynactin (610473, BD transduction Laboratories, 1:500), Gαi-1 (sc-56536, Santa Cruz Biotechnology, 1:200), and NuMA (ab36999, Abcam, 1:1,000). To test spindle orientation, cells were plated on L-patterned fibronectin coated coverslips (CYTOO). For RNAi experiments on patterned coverslips, cells were synchronized using a double thymidine block prior to plating.
Images were acquired on a DeltaVision Core microscope (Applied Precision) equipped with a CoolSnap HQ2 CCD camera. 30 to 40 Z-sections were acquired at 0.5-μm steps using Olympus 40x, 1.35 NA U-PlanApo, 20x, 0.75 NA UApo, or a 4x, 0.16 NA U-PlanSApo objective with 1×1 binning. Images were deconvolved using the DeltaVision software. Equivalent exposure conditions and scaling was used as appropriate. Fluorescence intensity and distance measurements were analyzed using Softworx (Applied Precision) and Metamorph (Molecular Devices). Line scans were generated using Metamorph (Molecular Devices). Kymographs were generated using Photoshop (Adobe).
Kiyomitsu T, & Cheeseman I.M. (2012). Chromosome and spindle pole-derived signals generate an intrinsic code for spindle position and orientation. Nature cell biology, 14(3), 311-317.
Publication 2012
Antibodies Cells Culture Media Dynactin Subunit 1 Elp1 protein, human Fluorescence Fluorescent Antibody Technique FN1 protein, human Hoechst33342 Homo sapiens Kymography Medical Devices Microscopy NUMA1 protein, human Radionuclide Imaging RNA Interference Thymidine Tubulin
3
HeLa Cell Culture and Staining Protocol
Cited 6 times
HeLa cells were cultured in DMEM supplemented with 10% (vol/vol) FBS (Gemini Bio Products), 25 mM Hepes, and GlutaMAX (Thermo Fisher Scientific). The transfection reagents used include Lipofectamine LTX (Thermo Fisher Scientific) and X-tremeGENE 9 (Roche). Cells were stained using Hoechst33342 (Thermo Fisher Scientific), MitoTracker deep red FM (Thermo Fisher Scientific), LysoTracker green DND-26 (Thermo Fisher Scientific), and Cascade blue–conjugated dextran (10,000 mol wt, anionic, lysine fixable; Thermo Fisher Scientific). The following rabbit monoclonal and polyclonal antibodies were used: HA (Cell Signaling Technology), GABARAPL1 (Abcam), GABARAPL2 (Abcam), PLEKHM1 (Abcam), LC3A (Cell Signaling Technology), LC3B (Cell Signaling Technology), LC3C (Cell Signaling Technology), LAMP1 (Cell Signaling Technology), GABARAP (Cell Signaling Technology), NDP52 (Cell Signaling Technology), OPTN (Proteintech), and Tom20 (Santa Cruz Biotechnology, Inc.). MFN1 and B17.2L antibodies were generated previously (Lazarou et al., 2007 (link), 2015 (link)). The polyclonal MFN1 and B17.2L antibodies were generated in rabbits using recombinant GST-Mfn1 (aa 667–741) and full-length B17.2L immunogens, respectively. The following mouse monoclonal antibodies were used: CoxII (Abcam), Hsp60 (Abcam), FLAG (Sigma-Aldrich), LAMP2 (Abcam), DNA (Progen), Actin (Abcam), p62 (Abnova), Parkin (Santa Cruz Biotechnology, Inc.), and Tom20 (Santa Cruz Biotechnology, Inc.). Chicken anti-GFP (Thermo Fisher Scientific) was also used. See Table S2 for catalog numbers.
Nguyen T.N., Padman B.S., Usher J., Oorschot V., Ramm G, & Lazarou M. (2016). Atg8 family LC3/GABARAP proteins are crucial for autophagosome–lysosome fusion but not autophagosome formation during PINK1/Parkin mitophagy and starvation. The Journal of Cell Biology, 215(6), 857-874.
Publication 2016
Actins Antibodies Antigens blue dextran Cells Chickens HeLa Cells HEPES Hoechst33342 LAMP2 protein, human Lipofectamine Lysine lysosomal-associated membrane protein 1, human LysoTracker Monoclonal Antibodies Mus Oryctolagus cuniculus PARK2 protein, human Progens PTGS2 protein, human Rabbits Transfection
4
Automated Image Quantification of Cellular Reporters
Cited 5 times
Image quantification was performed with CellProfiler version 2.1.1 (Kamentsky et al. 2011 (link)) with an in-house developed module implementing the watershed masked algorithm for segmentation (Di et al. 2012 (link)). The watershed separates an image in regions with single cells followed by pixel classification for each region as fore- or background, and this method performs well detecting the Hoechst33342 stained nuclei of the closely packed HepG2 cells. The binary mask containing the segmented nuclei was fed to the identify-primary-objects module, overlap-based-tracking module and intensity-nuclei-size-shape-measurement modules of CellProfiler. For the cytosol location of the Srxn1-GFP, Btg2-GFP and BiP-GFP reporters, the nuclear objects were used as seeds for the identify-secondary-objects module set to a propagation method with the MCT algorithm on adaptive (window size approximately 20 pixels) segmentation. The Keap1-GFP and 53bp1-GFP reporters are based on foci detection. The nuclei are segmented and used as seeds for the cytosol identification using the cytosolic GFP signal for the Keap1-GFP cell line. The foci detection is performed with the FociPicker3D plug-in (Du et al. 2011 (link)) in ImageJ, and each individual focus is assigned to either the nuclei (for 53bp1) or cytosol (Keap1) using the CellProfiler assign parent–child relationship module. The p21, p53, Nrf2, Xbp1, Atf4 and Chop reporters are based on quantifying the GFP signal in the nuclei. The nuclei signal is segmented, and these regions are directly used to quantify the GFP intensity. Segmentation results were stored as PNG files for quality control purposes, and CellProfiler pipelines were stored for reproducibility. Image analysis results were stored on the local machine as HDF5 files.
Wink S., Hiemstra S., Herpers B, & van de Water B. (2016). High-content imaging-based BAC-GFP toxicity pathway reporters to assess chemical adversity liabilities. Archives of Toxicology, 91(3), 1367-1383.
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Publication 2016
Acclimatization ATF4 protein, human Cell Nucleus Cells Cytosol DDIT3 protein, human Hoechst33342 KEAP1 protein, human NFE2L2 protein, human Plant Embryos Reproductive Techniques Signal Transduction TP53BP1 protein, human X-box binding protein 1, human
5
Shikonin Modulates NF-κB Activation
Cited 5 times

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Publication 2011
anti-IgG Cell Nucleus Cells Goat Hoechst33342 Immunoglobulins Macrophage Microscopy, Confocal Rabbits Rhodamine Serum Albumin, Bovine shikonin Sulfoxide, Dimethyl tetramethylrhodamine isothiocyanate Transcription Factor RelA Triton X-100

Most recents protocols related to «Hoechst33342»

1
Immunofluorescence Microscopy for CHIKV E2 Detection
Immunofluorescence microscopy (IFA) was performed essentially as described previously [14 (link)]. Primary antibodies used were rabbit polyclonal against CHIKV E2 [113 (link)] and mouse monoclonal antibody J2 against dsRNA (English & Scientific Consulting) diluted in 0.5% BSA in PBS. Primary antibodies were detected with donkey-a-rabbit-Cy3 or goat-a-mouse-Alexa488 (Jackson). Nuclei were stained with Hoechst33342. Coverslips were mounted with Prolong (Invitrogen) and examined using a Zeiss Axioskop2 fluorescence microscope with Axiocam HRc camera and AxioVision software.
Treffers E.E., Tas A., Scholte F.E., de Ru A.H., Snijder E.J., van Veelen P.A, & van Hemert M.J. (2023). The alphavirus nonstructural protein 2 NTPase induces a host translational shut-off through phosphorylation of eEF2 via cAMP-PKA-eEF2K signaling. PLOS Pathogens, 19(2), e1011179.
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Publication 2023
Antibodies Cell Nucleus Equus asinus Goat Hoechst33342 Immunofluorescence Microscopy Mice, House Microscopy, Fluorescence Monoclonal Antibodies Rabbits RNA, Double-Stranded
2
Exosome Uptake by Fibroblasts
Exosomes were fluorescently labeled using PKH67 membrane dye (Sigma). Then, the exosomes were cocultured with fibroblasts for 24 h. Nuclei were stained with Hoechst33342 for seconds, and the coverslips were sealed with resistant fluorescence quenching liquid. The fluorescence signals were analyzed by Leica SP5 confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany).
Zhang Q., Wang C., Li R., Liu J., Wang J., Wang T, & Wang B. (2023). The BAP31/miR-181a-5p/RECK axis promotes angiogenesis in colorectal cancer via fibroblast activation. Frontiers in Oncology, 13, 1056903.
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Publication 2023
Cell Nucleus Exosomes Fibroblasts Fluorescence Hoechst33342 Microscopy, Confocal Neoplasm Metastasis PKH67 Tissue, Membrane
3
EdU-Based Proliferation Assay
The EdU Apollo567 In Vitro kit (Ribobio, China) was used to assess cell proliferation. Cells transfected with miRNA mimics for 24 h were seeded into 6-well plates (2 × 105 cells/well). After culture for 12 h cultivated, the cells were transfected for 24 h and were cultivated for 40 h. The cells were then incubated in EdU working solution for 2 h, fixed with 4% paraformaldehyde, permeabilized, washed, and stained with 1× Apollo solution and 1× Hoechst33342 solution, according to the manufacturer’s instructions. Results were analyzed from microphotographs taken using a fluorescence microscope.
Wang X., Shi J., Huang M., Chen J., Dan J., Tang Y., Guo Z., He X, & Zhao Q. (2023). TUBB2B facilitates progression of hepatocellular carcinoma by regulating cholesterol metabolism through targeting HNF4A/CYP27A1. Cell Death & Disease, 14(3), 179.
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Publication 2023
Cell Proliferation Cells Hoechst33342 MicroRNAs Microscopy, Fluorescence paraform
4
Immunofluorescence Imaging of Respiratory and Sperm Cells
Respiratory and sperm cell samples were treated and incubated with primary and secondary antibodies as reported in previous studies (Omran and Loges, 2009 (link); Cindrić et al., 2019 (link); Aprea et al., 2021a (link)). Antibodies and their applied dilutions are listed in Supplementary Table S2. Cell nuclei were stained using Hoechst33342 (Thermo Fischer Scientific, Waltham, United States). Samples were mounted in DAKO fluorescence mounting medium (Dako North America Inc., Carpinteria, United States). IF images were taken with a Zeiss AxioObserver Z1 Apotome (Carl Zeiss Meditec AG, Oberkochen, Germany) or Laser Scanning Microscope LSM 880 (Carl Zeiss Microscopy GmbH, Jena, Germany). Images were processed and exported using the ZEISS ZEN Imaging Software 2012 (Carl Zeiss Microscopy GmbH, Jena, Germany) and figure panels created with the Adobe Creative Suite CS5 (Adobe Systems, San José, United States).
Aprea I., Wilken A., Krallmann C., Nöthe-Menchen T., Olbrich H., Loges N.T., Dougherty G.W., Bracht D., Brenker C., Kliesch S., Strünker T., Tüttelmann F., Raidt J, & Omran H. (2023). Pathogenic gene variants in CCDC39, CCDC40, RSPH1, RSPH9, HYDIN, and SPEF2 cause defects of sperm flagella composition and male infertility. Frontiers in Genetics, 14, 1117821.
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Publication 2023
Antibodies Cell Nucleus Fluorescence Hoechst33342 Laser Scanning Microscopy Microscopy Respiratory Rate Sperm Technique, Dilution
5
Alzheimer's Disease Biomarker Assay
Pectinase, cellulase, β-galactosidase, dextranase, β-mannase, and standards for maltose (purity ≥98%), maltotriose (purity ≥98%), malttetraose (purity ≥97%), and maltpentaose (purity ≥7%) were products of Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). D-glucose standard (purity ≥98%) was obtained from Sichuan Weikeqi Biotechnology Co., Ltd. (Chengdu, China). Moreover, 8-aminonaphthalene-1,3,6-trisulfonic acid (ANTS) was purchased from Meryer (Shanghai, China) Biochemical Technology Co., Ltd. (Shanghai, China). Aβ25–35 and dimethylsulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was obtained from Gibco (Burlington, ON, Canada), Annexin V-APC/PI and Hoechst33342/PI dual staining kits were from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing, China), Dulbecco’s modified Eagles medium (DMEM) cell culture medium was purchased from Procell Life Science &Technology Co., Ltd. (Wuhan, China). Trypsin-EDTA from Hyclone Laboratories (Logan, UT, USA). Detection kits for ROS, SOD, LDH and MDA were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). TRIzol reagent and the RevertAid First Strand cDNA Synthesis Kit was purchased from Thermo Fisher (Waltham, MA, USA).
Hu M.B., Gao K.X., Wang Y, & Liu Y.J. (2023). Characterization of Polysaccharides from the Pericarp of Zanthoxylum bungeanum Maxim by Saccharide Mapping and Their Neuroprotective Effects. Molecules, 28(4), 1813.
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Publication 2023
1-Naphthylamine Acids Anabolism Annexin A5 Ants beta-Galactosidase beta Mannosidase Cells Cellulase Culture Media Dextranase DNA, Complementary Eagle Edetic Acid Fetal Bovine Serum Glucose Hoechst33342 Maltose maltotriose Polygalacturonase Sulfoxide, Dimethyl trizol Trypsin

Top products related to «Hoechst33342»

1
Hoechst 33342 by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, China, France, Canada, Spain, Belgium, Italy, Australia, Austria, Denmark, Netherlands, Switzerland, Ireland, New Zealand, Portugal, Brazil, Argentina, Singapore, Poland, Ukraine, Macao, Thailand, Finland, Lithuania, Sweden
Hoechst 33342 is a fluorescent dye that binds to DNA. It is commonly used in various applications, such as cell staining and flow cytometry, to identify and analyze cell populations.
2
Hoechst 33342 by Merck Group
Sourced in United States, Germany, Italy, United Kingdom, Japan, China, France, Sao Tome and Principe, Canada, Macao, Belgium, Israel, Spain, Australia, Poland, Switzerland, India, Denmark, Austria, Netherlands
Hoechst 33342 is a fluorescent dye that binds to DNA. It can be used to visualize and quantify nucleic acids in various applications such as flow cytometry, fluorescence microscopy, and nucleic acid staining.
3
Hoechst 33342 by Beyotime
Sourced in China, United States, Japan, Germany
Hoechst 33342 is a fluorescent dye that binds to the minor groove of DNA. It is commonly used in cell biology and microscopy applications to stain and visualize cell nuclei.
4
Hoechst 33342 by Dojindo Laboratories
Sourced in Japan, United States, China
Hoechst 33342 is a fluorescent dye that binds to the minor groove of DNA. It is commonly used in various biological applications for the detection and analysis of cell nuclei.
5
Triton x 100 by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, China, Japan, France, Canada, Sao Tome and Principe, Switzerland, Macao, Poland, Spain, Australia, India, Belgium, Israel, Sweden, Ireland, Denmark, Brazil, Portugal, Panama, Netherlands, Hungary, Czechia, Austria, Norway, Slovakia, Singapore, Argentina, Mexico, Senegal
Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.
6
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
7
Propidium iodide by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Macao, Canada, Spain, India, Belgium, Australia, Israel, Switzerland, Poland, Ireland, Argentina, Austria, Brazil, Sweden, Portugal, New Zealand, Netherlands, Slovakia, Norway, Hungary, Czechia, Denmark
Propidium iodide is a fluorescent dye commonly used in molecular biology and flow cytometry applications. It binds to DNA and is used to stain cell nuclei, allowing for the identification and quantification of cells in various stages of the cell cycle.
8
Alexa fluor 488 by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Japan, France, Italy, Canada, China, Spain, Switzerland, Denmark, Australia, Hungary, Belgium, Ireland, Israel, Netherlands, Moldova, Republic of, India, Austria, Czechia, Poland
Alexa Fluor 488 is a fluorescent dye used in various biotechnological applications. It has an excitation maximum at 495 nm and an emission maximum at 519 nm, producing a green fluorescent signal. Alexa Fluor 488 is known for its brightness, photostability, and pH-insensitivity, making it a popular choice for labeling biomolecules in biological research.
9
Lsm 710 by Zeiss
Sourced in Germany, United States, United Kingdom, Japan, Switzerland, France, China, Canada, Italy, Spain, Singapore, Austria, Hungary, Australia
The LSM 710 is a laser scanning microscope developed by Zeiss. It is designed for high-resolution imaging and analysis of biological and materials samples. The LSM 710 utilizes a laser excitation source and a scanning system to capture detailed images of specimens at the microscopic level. The specific capabilities and technical details of the LSM 710 are not provided in this response to maintain an unbiased and factual approach.
10
Bovine serum albumin (bsa) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico
Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.

More about "Hoechst33342"

Hoechst 33342, also known as bisbenzimide or Hoechst 33258, is a widely used fluorescent dye in cell biology research.
This DNA-binding dye emits a bright blue fluorescence when excited, making it a popular choice for staining cell nuclei and tracking cell division and apoptosis.
Hoechst 33342 binds to the minor groove of DNA, allowing researchers to visualize and analyze cellular processes with high specificity and accuracy.
In addition to Hoechst 33342, researchers may also utilize other fluorescent dyes and reagents, such as Triton X-100 (a non-ionic detergent used for cell permeabilization), FBS (fetal bovine serum, a common cell culture supplement), propidium iodide (a red fluorescent dye that binds to DNA), and Alexa Fluor 488 (a green fluorescent dye used for labeling proteins or antibodies).
These tools, combined with advanced imaging techniques like confocal microscopy (e.g., LSM 710), enable comprehensive investigation of cellular dynamics and structures, including the identification of apoptotic cells and the quantification of cell proliferation.
The PubCompare.ai platform helps researchers discover the optimal Hoechst33342 protocols by leveraging data-driven comparisons of scientific literature, preprints, and patents.
This AI-driven platform enables users to locate and compare Hoechts33342 protocols, ensuring they have access to the most up-to-date and effective solutions for their specific experimental requirements.
By incorporating insights from various related terms and techniques, researchers can optimize their use of Hoechst 33342 and other fluorescent dyes to answer their scientific questions with greater precision and efficiency.