The largest database of trusted experimental protocols
> Chemicals & Drugs > Organic Chemical > Hydroxytyrosol

Hydroxytyrosol

Hydroxytyrosol is a polyphenolic compound found in olive oil and other plant sources.
It has been studied for its potential health benefits, including antioxidant, anti-inflammatory, and cardioprotective effects.
Researchers use PubCompare.ai's AI-powered platform to locate the most reproducible and accurate protocols for extracting and analyzing hydroxytyrosol.
The platform scans literature, pre-prints, and patents to identify the best methods and products, using intelligent comparisons to ensure optimal results.
Experince the power of this AI-driven research solution today and discover the latest insights on hydroxytyrosol.

Most cited protocols related to «Hydroxytyrosol»

Immunohistochemical analysis was performed as already described [54 (link)]. Sections were probed overnight with anti-zonula occludens (ZO) antibody (1:100; Millipore, Abingdon, UK) or anti-occludin antibody (1:100; Santa Cruz Biotechnology) or anti-intracellular adhesion molecule-1 (ICAM-1) (1:100; Santa Cruz Biotechnology) or anti-P-selectin (1:100; Santa Cruz Biotechnology). Sections were washed with phosphate-buffer saline (PBS) and incubated with peroxidase-conjugated bovine anti-mouse IgG, secondary antibody (1:2000 Jackson Immuno Research, WestGrove, Pennsylvania, USA). Specific labeling was provided with a biotin-conjugated goat anti-mouse IgG and avidin-biotin peroxidase complex (Vector Laboratories, Burlingame, CA, USA). Images were collected using a Leica DM6 (Milan, Italy) microscope. The histogram profile reports the positive pixel intensity value taken from a computer program.
Full text: Click here
Publication 2020
anti-IgG Antibodies, Anti-Idiotypic Avidin Biotin Buffers Cattle Cell Adhesion Molecules Cloning Vectors Goat Immunoglobulins Microscopy Mus Occludin Peroxidase Phosphates Protoplasm Saline Solution SELP protein, human Tight Junctions

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2014
Agaricales Biological Assay Buffers dopachrome kojic acid Levodopa Monophenol Monooxygenase Phosphates Psychological Inhibition Sulfoxide, Dimethyl

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2014
Agaricales Biological Assay Buffers Cells Centrifugation dopachrome Enzymes H 450 kojic acid Levodopa Melanoma, B16 Monophenol Monooxygenase Phosphates Proteins Serum Albumin, Bovine Triton X-100 Vision
The superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S-transferase (GST) activity was evaluated as already described previously [47 ,48 (link)]. SOD activity was evaluated by quantitative sandwich enzyme immunoassay technique using a commercial kit (Cusabio, Houston, Texas). The results were expressed as U/mg of protein. For GPx activity, the absorbance was monitored at 340 nm at 37 °C for 10 min, and the results were expressed as μmol of reduced glutathione (GSH)/min/mg of protein. The GR activity was evaluated by measuring the consumption of nicotinamide adenine dinucleotide phosphate (NADPH) as a cofactor in the reduction of oxidized glutathione to reduce GSH. The results were expressed as U of GR/mg of protein. One U of enzyme activity was defined as the amount of GR that oxidizes 1 μmol of NADPH per min. The GST activity was measured using 1-chloro-2,4-dinitrobenzene (CDNB) as a substrate. The results were expressed as U of GST/mg of protein. One U of enzyme activity was defined as the amount of GST that produces 1 μmol of the conjugate of GSH with CDNB per min. The total protein concentration in the homogenate was measured using the method of Bradford.
Full text: Click here
Publication 2020
Dinitrobenzenes enzyme activity Enzyme Immunoassay Glutathione Disulfide Glutathione Reductase Glutathione S-Transferase NADP Peroxidase, Glutathione Proteins Reduced Glutathione Superoxide Dismutase
Western blots were performed as described in our previous studies [55 (link)]. Pancreas and colon tissue from each mouse were suspended in an extraction’s buffer containing 0.15 µM pepstatin A, 0.2 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM sodium orthovanadate and 20 µM leupeptin, homogenized at the highest setting for 2 min and centrifuged at 1000× g for 10 min at 4 °C. Supernatants contained the cytosolic fractions, while the pellets represented the nuclear ones. Pellets were resuspended in a second buffer containing 150 mM sodium chloride (NaCl), 1% Triton X-100, 1 mM ethylene glycol tetraacetic acid (EGTA), 10 mM tris–chloridric acid (HCl) pH 7.4, 0.2 mM PMSF, 1 mM Ethylenediaminetetraacetic acid (EDTA), 0.2 mM sodium orthovanadate and 20 µm leupeptin. After centrifugation at 4 °C for 30 min, the nuclear proteins containing the supernatants were stored at −80 °C for further analysis. Specific primary antibody anti-Nrf2 (1:1000, Santa Cruz Biotechnology) or anti-HO-1 (1:1000; Santa Cruz Biotechnology) was mixed in 1× PBS, 5% w/v nonfat dried milk and 0.1% Tween-20 and incubated at 4 °C overnight. After, blots were incubated with peroxidase-conjugated bovine anti-mouse IgG secondary antibody or peroxidase-conjugated goat anti-rabbit IgG (1:2000, Jackson Immuno Research) for 1 h at room temperature. To verify the equal amounts of protein, membranes were also incubated with the antibody against laminin (1:1000; Santa Cruz Biotechnology) and β-actin (1: 1000; Santa Cruz Biotechnology). Signals were identified with enhanced chemiluminescence (ECL) detection system reagent and the relative expression of the protein bands was measured by densitometry with BIORAD ChemiDocTM XRS+software (Bio-rad, Milan, Italy). A representation of blot signals was imported to analysis software (Image Quant TL, v2003).
Full text: Click here
Publication 2020
Acids Actins anti-IgG Bos taurus Buffers Centrifugation Chemiluminescence Colon Cytosol Densitometry Edetic Acid Egtazic Acid Goat Immunoglobulins Laminin leupeptin Milk, Cow's Mus NFE2L2 protein, human Nuclear Proteins Orthovanadate Pancreas Pellets, Drug pepstatin Peroxidase Phenylmethylsulfonyl Fluoride Proteins Rabbits Sodium Sodium Chloride Tissue, Membrane Tissues Triton X-100 Tromethamine Tween 20 Western Blot

Most recents protocols related to «Hydroxytyrosol»

Hydroxytyrosol and its metabolites
in plasma were determined by liquid–liquid extraction followed
by LC-MS/MS analysis as previously described.11 (link) Briefly, 200 μL of plasma was mixed with 10 μL of freshly
prepared 10% ascorbic acid followed by 10 μL of 0.5% acetic
acid and 10 μL of 2-(3-hydroxyphenyl) ethanol (10 μM,
I.S.). Then, 2 mL of ethyl acetate was added to the samples that were
vigorously mixed in a vortex (5 min), placed into an ultrasonic bath
(10 min), and centrifuged at 1500g at 4 °C (15
min) (Centrifuge Megafuge 1.0R). A second extraction of the pellet
with 2 mL of ethyl acetate was carried out. Subsequently, the two
pooled supernatants were evaporated to dryness, reconstituted with
100 μL of 80% methanol, placed into amber vials, and immediately
analyzed by LC-MS/MS.
An Agilent 1260 liquid chromatograph (Agilent
Technologies, Santa Clara, USA) coupled to a QTRAP 4000 mass spectrometer
(AB Sciex, Toronto, Canada) equipped with a Turbo V electrospray ionization
(ESI) source was used for the determination of hydroxytyrosol and
its metabolites. Analyst software, version 1.6.2. (AB Sciex) operated
the instrument and was employed for data analysis. The equipment was
located at the Scientific and Technological Centre of the Universitat
de Barcelona (CCiTUB).
Injections of 2 μL of each sample
were performed by an automated
autosampler that maintained vials at 10 °C to avoid degradation.
Chromatographic separation of hydroxytyrosol and its metabolites was
performed in a Zorbax Eclipse XDB-C18 reversed-phase column (150
mm × 4.6 mm, 5 μm) protected with a guard cartridge of
the same material (Zorbax Eclipse XDB-C18, 12.5 mm × 4.6 mm,
5 μm) with the temperature set at 30 °C. The mobile phase
consisted of phase A formed by Milli-Q water with 0.025% acetic acid
and phase B containing acetonitrile with 5% acetone delivered at a
flow of 0.8 mL/min. The following gradient elution was used: 0 min,
95% A and 5% B; 1 min, 90% A and 10% B; 10 min, 35% A and 65% B; 10.5
min, 0% A and 100% B. Solvent B was maintained at 100% for 5 min to
prevent carryover prior to returning to initial conditions. A 6 min
delay was programmed before the next injection to ensure the equilibration
of the system. Moreover, the injector needle was washed with 1:1:1
(v/v) 2-propanol, tetrahydrofuran, and Milli-Q water to avoid further
carryover.
The ESI source, operating in negative mode, was set
as follows:
temperature, 600 °C; curtain gas (N2), 25 arbitrary
units (au); ion source gas 1 (source heating gas, N2);
50 au; ion source gas 2 (drying gas, N2); 50 au, and ionization
spray voltage, −3500 V. The MS analysis was performed in multiple
reaction monitoring (MRM) mode and the specific parameters are shown
in Figure 1. Within
each analytical run, a full set of calibration standards was injected
including a reagent blank and blank plasma.
The plasmatic concentrations were calculated by
the interpolation
of the peak area ratio of hydroxytyrosol versus I.S. on a calibration
curve. Calibration standards were constructed with blank plasma obtained
by cardiac puncture from overnight fasted rats that had never received
either table olives or hydroxytyrosol. Then, 190 μL of blank
plasma was spiked with 10 μL of working standards at 0, 200,
500, 1000, 2000, 3000, and 5000 nmol/L to obtain the final concentrations
of 0, 10, 25, 50, 100, 150, and 250 nmol/L. Metabolites were identified
with the m/z indicated in Figure 1 and were assumed
to possess a LC-MS/MS response similar to that of hydroxytyrosol.
Hence, the concentrations of the sulfate and glucuronide derivatives
were quantified using the standard curve of the parent compound. Results
were expressed in nmol per liter of plasma (nmol/L). The method was
validated following the EMA guidelines33 at six different concentrations ranging from 0 to 250 nmol/L analyzed
on three different days. Validation results indicated that the analytical
method is linear (R2 > 0.998), precise
(CV < 15%), with satisfactory recovery (98.4 ± 1.64%), absence
of matrix effect (96.7 ± 2.75%), no carry-over, and adequate
sensibility with a limit of quantification (LOQ) of 0.2 nmol/L.
Full text: Click here
Publication 2024
Bovine Serum Albumin, fluorescein isothiocyanate-dextran (wt 4,000), dimethyl-sulfoxide (DMSO), Bradford reagent, Cell Lytic-M, lipopolysaccharide from Escherichia coli, hydroxytyrosol, tyrosol, NaCl, NaF, K2HPO4, KH2PO4, MgSO4x7H2O, CaCl2x6H2O, NaHCO3, Tween 80 and all solvents of analytical grade were purchased from Sigma Aldrich (Milano, Italy). tyrosol glucuronide, tyrosol sulfate sodium salt, 3′- hydroxytyrosol 3′-glucuronide, hydroxytyrosol 3-sulfate sodium salt, were obtained from LGC standards (Sesto San Giovanni, Italy). The Phosphatase and Protease Inhibitor Cocktail, nitrocellulose membranes, gels and all material for electrophoresis were purchased from ThermoFisher Scientific (Massachusetts, United States). ReagentPack Subculture Reagents with Trypsin/EDTA, TNS (Trypsin Neutralizer solution) and HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid) solutions were obtained from Lonza (Basel, Switzerland). Transwell inserts were obtained from Corning Costar Corp. (New York, N.Y., United States).
Full text: Click here
Publication 2024
The OMWW extract enriched in polyphenols (hydroxytyrosol and tyrosol) was provided by Stymon Natural Products P.C., Patras, Greece (www.stymon.com, accessed on 21 December 2023). This product derives from OMWW of the olive (Olea Europaea L.) variety Koroneiki and is produced based on a unique patent (Patent number 1,010,150 IOBE (INT.CL.2021.01) A23L 19/00 A23L 33/105, Stymonphen Liquid) using only green technologies. Its total polyphenol content was equal to 15,000 ± 592 mg/kg, according to the Folin–Ciocalteu method [66 (link)]; hydroxytyrosol and tyrosol were the main phenolic compounds (8784 mg/kg and 1638 mg/kg, respectively), detected by HPLC-DAD [67 ]. The stock solution was filtered, vortexed, and diluted in phosphate-buffered saline (PBS, Euroclone, Milan, Italy) to reach 1400 µg/mL; from this, different scalar concentrations of polyphenols (0.35; 0.7; 7; 14; 70; 140 µg) were obtained for the successive analyses by diluting them in complete culture medium.
Full text: Click here
Publication 2024
All chemicals for the following assays were purchased from Sigma-Aldrich, Munich, Germany, except for the HT and OLE reference standards, which were purchased from ExtraSynthase (Lyon Nord, France).
Full text: Click here
Publication 2024
Lipopolysaccharide (LPS, L4391-1MG), L-glutamic acid (G8415), and a hydrogen peroxide solution (30%w/w, H1009) were sourced from Sigma-Aldrich (United States). Recombinant murine IL-1β (211-11B) was procured from PEPROTECH (United States). Fluoxetine HCl (S27345, purity >98%), was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. Hydroxytyrosol (purity >98%) was synthesized and purified by the Biopharmaceutical Laboratory of Shandong Academy of Pharmaceutical Sciences (China). Detailed structural and chromatographic information for HT is shown in Supplementary Figure S1.
Full text: Click here
Publication 2024

Top products related to «Hydroxytyrosol»

Sourced in United States, Germany, Italy, France, Spain
Hydroxytyrosol is a natural compound found in olive oil. It is a polyphenol with antioxidant properties. Hydroxytyrosol is used in laboratory research and testing.
Sourced in United States, Germany, Italy, France, Spain
Oleuropein is a chemical compound found in the leaves and fruit of olive trees. It is a complex phenolic compound with various biological properties. Oleuropein is used in laboratory research applications, but a detailed description of its core function cannot be provided in a concise, unbiased, and factual manner without risk of extrapolation or interpretation.
Sourced in United States, Germany, Italy, Spain, France, India, China, Poland, Australia, United Kingdom, Sao Tome and Principe, Brazil, Chile, Ireland, Canada, Singapore, Switzerland, Malaysia, Portugal, Mexico, Hungary, New Zealand, Belgium, Czechia, Macao, Hong Kong, Sweden, Argentina, Cameroon, Japan, Slovakia, Serbia
Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
Sourced in United States, Germany, Italy, France, Switzerland, Spain
Tyrosol is a laboratory reagent used for the detection and quantification of tyrosine in biological samples. It functions as a colorimetric assay based on the reaction between tyrosine and Tyrosol, resulting in the production of a colored complex that can be measured spectrophotometrically.
Sourced in United States, Germany, Italy, Poland, Spain, China, France, India, Sao Tome and Principe, United Kingdom, Malaysia, Portugal, Australia, Switzerland, Macao
Luteolin is a laboratory equipment product manufactured by Merck Group. It is a flavonoid compound used as a chemical standard and reference material for analytical and research applications.
Sourced in United States, Germany, Italy, France, Poland, Spain, China, United Kingdom, Australia, Sao Tome and Principe, Switzerland, India, Ireland, Canada, Macao, Brazil, Austria, Mexico, Czechia, Portugal
Caffeic acid is a phenolic compound commonly found in various plants. It serves as a laboratory standard for the identification and quantification of similar phenolic compounds using analytical techniques such as high-performance liquid chromatography (HPLC) and spectrophotometry.
Sourced in France, Switzerland, Germany
Hydroxytyrosol is a natural phenolic compound found in olive oil. It is a powerful antioxidant that helps protect cells from oxidative stress. Hydroxytyrosol can be used in various applications, such as dietary supplements and cosmetic products.
Sourced in United States, Germany, Italy, Spain, France, China, Poland, United Kingdom, Sao Tome and Principe, Switzerland, Canada, Ireland, India, Australia, Japan, Macao, Portugal
P-coumaric acid is a naturally occurring phenolic compound that can be utilized as a reference standard or an analytical reagent in various laboratory settings. It is a white to off-white crystalline solid that is soluble in organic solvents. P-coumaric acid is commonly used as a standard in analytical techniques, such as high-performance liquid chromatography (HPLC) and spectrophotometric measurements, to quantify and characterize similar compounds in sample matrices.
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
Sourced in France, United States, Italy, China, Finland
Oleuropein is a natural compound found in the leaves and fruit of olive trees. It is a key active ingredient used in laboratory research and analysis. Oleuropein has well-documented antioxidant and anti-inflammatory properties. Further details on its specific functions or applications are not available.

More about "Hydroxytyrosol"

Hydroxytyrosol is a polyphenolic compound found in olive oil and other plant sources, such as olives, olive leaves, and red wine.
It has been extensively studied for its potential health benefits, including its antioxidant, anti-inflammatory, and cardioprotective properties.
Researchers often utilize PubCompare.ai's AI-powered platform to locate the most reproducible and accurate protocols for extracting and analyzing hydroxytyrosol.
The PubCompare.ai platform scans a vast array of literature, pre-prints, and patents to identify the best methods and products for hydroxytyrosol research.
By using intelligent comparisons, the platform ensures that researchers can access the optimal strategies for their experiments, leading to more reliable and meaningful results.
Hydroxytyrosol is closely related to other polyphenolic compounds like oleuropein, gallic acid, tyrosol, luteolin, caffeic acid, and p-coumaric acid, all of which have been studied for their potential health benefits.
These compounds are often found in similar plant sources and share some common biochemical properties.
The extraction and analysis of hydroxytyrosol can be challenging, as it requires careful optimization of factors like solvent selection (e.g., methanol), extraction time, and purification techniques.
PubCompare.ai's AI-driven research solutions can help researchers navigate these complexities and identify the most reproducible and accurate protocols, ultimately advancing the understanding of hydroxytyrosol and its potential applications in various fields, such as nutrition, medicine, and cosmetics.