Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Organic Chemical > Hypaque

Hypaque

Hypaque is a non-ionic, water-soluble, iodinated contrast medium used for radiographic imaging procedures.
It is commonly employed in angiography, urography, and other diagnostic tests to enhance visibility of blood vessels, organs, and other structures.
Hypaque provides superior contrast and reduced side effects compared to older ionic contrast agents.
Researchers can utilize PubCompare.ai's AI-powered platform to streamline their Hypaque-related research, easily locating relevant protocols from literature, preprints, and patents, while leveraging automated comparisons to identify the most optimal protocols and products.
This innovative solution helps researchers optimize their Hypaque studies and advance their work more efficiently.

Most cited protocols related to «Hypaque»

1
Isolation and Sorting of Hematopoietic Cell Populations
Cited 78 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2011
Antibodies B-Lymphocytes Basophils Cells Dendritic Cells Eosinophil Ficoll Flow Cytometry Granulocyte Progenitor Cells Hypaque Immunophenotyping ITGAM protein, human Lymph Megakaryocyte-Erythroid Progenitor Cells Megakaryocytes Monocytes Myeloid Progenitor Cells Natural Killer Cells Neutrophil Population Group Stem Cells, Hematopoietic T-Lymphocyte trizol
2
Isolation and Infection of CD4+ T Cells
Cited 13 times
Human tonsil or splenic tissues were obtained from the National Disease Research Interchange and the Cooperative Human Tissue Network and processed as previously described11 (link). Dead cells within the complete HLACs were first removed by Ficoll-Hypaque gradient centrifugation. CD4 T cells (CD3+) were isolated from HLACs by positive selection using CD4 microbeads (Miltenyi) as described11 (link). CCR5-expressing CD4 T cells were positively separated from CD4 T-cell cultures (PlusCellect R&D Systems Cat # PLS180), negatively isolated from complete HLACs (STEMCELL Technologies, EasySep™ Human CD4+ T-cell Enrichment Kit). In shRNA experiments, infections with R5-tropic HIV-1, and when splenic cells that are extremely refractory to HIV-1 infection were used, we modified the infection system by overlaying HLAC cells on a monolayer of 293T cells that had been transfected with HIV-1 proviral clones, as previously described11 (link). Flow cytometry data were collected on a FACS Calibur (BD Biosciences) and analyzed with FlowJo software (Treestar). HIV-1 viruses were generated by transfection of proviral DNA into 293T cells using calcium phosphate.
Doitsh G., Galloway N.L., Geng X., Yang Z., Monroe K.M., Zepeda O., Hunt P.W., Hatano H., Sowinski S., Muñoz-Arias I, & Greene W.C. (2014). Pyroptosis drives CD4 T-cell depletion in HIV-1 infection. Nature, 505(7484), 509-514.
Publication 2014
Calcium Phosphates CCR5 protein, human CD4 Positive T Lymphocytes Centrifugation Clone Cells Ficoll Flow Cytometry HEK293 Cells HIV-1 HIV Infections Homo sapiens Hypaque Microspheres Palatine Tonsil Proviruses Sepsis Short Hairpin RNA Spleen Stem Cells Tissues Transfection Virus
3
Isolation and Culture of Diverse Cell Lines
Cited 12 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2011
Breast Cells Cell Separation Centrifugation, Density Gradient Donors Embryo Epithelial Cells Ficoll Gastric Cancer HEK293 Cells Hypaque Kidney Malignant Neoplasm of Breast MBD protocol MCF-7 Cells Myeloproliferative Syndrome, Transient Neoplasms Patients Percoll
4
Isolation of Normal-Density Neutrophils
Cited 10 times
Normal-density neutrophils were isolated by dextran sedimentation of RBC pellets as described (14 ). The cells were collected by centrifugation and contained over 95% neutrophils. In additional experiments, and to exclude an effect of the isolation technique on the differences observed between normal-density neutrophils and LDGs, an alternative isolation procedure was performed. After collecting the PBMC fraction, the residual Ficoll/Hypaque suspension was harvested and slowly layered on equal volumes of Histopaque-1119 (Sigma). Neutrophils were recovered by centrifugation at 1440 rpm for 30 min at room temperature. The neutrophil fraction was recovered from the interface, washed twice with PBS and once with ice-cold PBS/2mM EDTA/0.5% BSA. The supernatant was then harvested and the cell pellet was incubated with an Ab cocktail similar to the one used to isolate LDGs by negative selection, as specified above. This was followed by negative selection with magnetic beads, identical to the procedure used to isolate LDGs. The purity of the neutrophil fraction also typically exceeded 95% and was determined by CD15, CD14 and CD10 expression by FACS, as detailed above.
Denny M.F., Yalavarthi S., Zhao W., Thacker S.G., Anderson M., Sandy A.R., McCune W.J, & Kaplan M.J. (2010). A distinct subset of proinflammatory neutrophils isolated from patients with systemic lupus erythematosus induces vascular damage and synthesizes type I Interferons. Journal of immunology (Baltimore, Md. : 1950), 184(6), 3284-3297.
Publication 2010
Cells Centrifugation Cold Temperature Dextran Edetic Acid Ficoll histopaque Hypaque isolation Neutrophil Pellets, Drug
5
Longitudinal Study of Breast Cancer Micrometastases
Cited 9 times
A longitudinal trial for the study of micrometastatic disease in breast cancer has been underway in our institution since 1996. Peripheral blood samples are obtained from patients who provide their written informed consent as part of the routine evaluation before the initiation of and at the end of adjuvant treatment, as well as prior to and after the completion of each chemotherapy line in patients with metastatic disease. Frozen RNA samples and peripheral blood mononuclear cell (PBMC) cytospins are prepared simultaneously and stored at -80°C until use. In the current trial, samples taken from patients with adjuvant or metastatic breast cancer were screened for cytokeratin (CK)-19 mRNA expression [41 (link),42 (link)] by RT-PCR. Fifty CK-19 mRNA-positive patients (25 with early stage breast cancer and 25 with metastatic breast cancer) were enrolled in the present study. We used archived slides from these patients because we wanted to have the same blood samples for real-time RT-PCR and immunocytochemistry. Ten healthy female blood donors were also included as a control group. All blood samples were obtained at the middle of venipuncture after the first 5 mL of blood were discarded. These precautions were undertaken to avoid contamination of the blood samples with epithelial cells from the skin during sample collection. All patients and healthy volunteers gave their written informed consent to participate in the study, which has been approved by the Ethics and Scientific Committees of our institution.
The volume of blood drawn from each patient was 20 mL each for immunofluorescence and RT-PCR experiments and 20 mL for immunomagnetic isolation. PBMCs were isolated by Ficoll-Hypaque density gradient centrifugation (d = 1,077 g/mol) at 1,800 rpm for 30 minutes. PBMCs were washed three times with PBS and centrifuged at 1500 rpm for 10 minutes. Aliquots of 250,000 cells were cytocentrifuged at 2,000 rpm for 2 minutes on glass slides. Cytospins were dried up and stored at -80°C. Four to five slides from each patient were used for staining experiments, so 1 × 106 PBMCs per patient were scanned.
Kallergi G., Papadaki M.A., Politaki E., Mavroudis D., Georgoulias V, & Agelaki S. (2011). Epithelial to mesenchymal transition markers expressed in circulating tumour cells of early and metastatic breast cancer patients. Breast Cancer Research : BCR, 13(3), R59.
Full text: Click here
Publication 2011
BLOOD Blood Volume Breast Cells Centrifugation, Density Gradient Donor, Blood Epithelial Cells Ficoll Freezing Healthy Volunteers Hypaque Immunocytochemistry Immunofluorescence isolation Keratin-19 Malignant Neoplasm of Breast Neoplasm Metastasis Patients PBMC Peripheral Blood Mononuclear Cells Pharmaceutical Adjuvants Pharmacotherapy Phlebotomy Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Skin Specimen Collection Woman

Most recents protocols related to «Hypaque»

1
Llama Nanobody Isolation Protocol
Not available on PMC !

EXAMPLE 36

Peripheral blood mononuclear cells were prepared from blood samples obtained from llama No. 45 and No. 46 using Ficoll-Hypaque according to the manufacturer's instructions. Next, total RNA extracted was extracted from these cells and used as starting material for RT-PCR to amplify Nanobody encoding gene fragments. These fragments were cloned into phagemid vector pAX50. Phage was prepared according to standard methods (see for example the prior art and applications filed by applicant cited herein) and stored after filter sterilization at 4° C. for further use.

US11858997B2. Amino acid sequences that modulate the interaction between cells of the immune system (2024-01-02). Ablynx N.V. [BE]. Inventors: Guy Hermans [BE], Peter Verheesen [BE], Edward Dolk [NL], Hendricus Renerus Jacobus Mattheus Hoogenboom [NL], Michael John Scott Saunders [BE], Hans De Haard [NL], Renee de Bruin [NL].
Full text: Click here
Patent 2024
Bacteriophages BLOOD Cloning Vectors DNA Library Ficoll Genes Hypaque Llamas PBMC Peripheral Blood Mononuclear Cells Reverse Transcriptase Polymerase Chain Reaction Sterilization
2
Llama Nanobody Library Generation
Not available on PMC !

EXAMPLE 12

Peripheral blood mononuclear cells were prepared from blood samples obtained from llama No. 146 and No. 147 using Ficoll-Hypaque according to the manufacturer's instructions. Next, total RNA extracted was extracted from these cells and used as starting material for RT-PCR to amplify Nanobody encoding gene fragments. These fragments were cloned into phagemid vector pAX50. Phage was prepared according to standard methods (see for example the prior art and applications filed by applicant cited herein) and stored after filter sterilization at 4° C. for further use.

US11858997B2. Amino acid sequences that modulate the interaction between cells of the immune system (2024-01-02). Ablynx N.V. [BE]. Inventors: Guy Hermans [BE], Peter Verheesen [BE], Edward Dolk [NL], Hendricus Renerus Jacobus Mattheus Hoogenboom [NL], Michael John Scott Saunders [BE], Hans De Haard [NL], Renee de Bruin [NL].
Full text: Click here
Patent 2024
Bacteriophages BLOOD Cloning Vectors DNA Library Ficoll Genes Hypaque Llamas PBMC Peripheral Blood Mononuclear Cells Reverse Transcriptase Polymerase Chain Reaction Sterilization
3
Llama-Derived Nanobody Preparation
Not available on PMC !

EXAMPLE 30

Peripheral blood mononuclear cells were prepared from blood samples obtained from llama No. 149 using Ficoll-Hypaque according to the manufacturer's instructions. Next, total RNA extracted was extracted from these cells and used as starting material for RT-PCR to amplify Nanobody encoding gene fragments. These fragments were cloned into phagemid vector pAX50. Phage was prepared according to standard methods (see for example the prior art and applications filed by applicant cited herein) and stored after filter sterilization at 4° C. for further use.

US11858997B2. Amino acid sequences that modulate the interaction between cells of the immune system (2024-01-02). Ablynx N.V. [BE]. Inventors: Guy Hermans [BE], Peter Verheesen [BE], Edward Dolk [NL], Hendricus Renerus Jacobus Mattheus Hoogenboom [NL], Michael John Scott Saunders [BE], Hans De Haard [NL], Renee de Bruin [NL].
Full text: Click here
Patent 2024
Bacteriophages BLOOD Cloning Vectors DNA Library Ficoll Genes Hypaque Llamas PBMC Peripheral Blood Mononuclear Cells Reverse Transcriptase Polymerase Chain Reaction Sterilization
4
Nanobody Encoding Gene Amplification
Not available on PMC !

EXAMPLE 24

Peripheral blood mononuclear cells were prepared from blood samples obtained from llama No. 149 using Ficoll-Hypaque according to the manufacturer's instructions. Next, total RNA extracted was extracted from these cells and used as starting material for RT-PCR to amplify Nanobody encoding gene fragments. These fragments were cloned into phagemid vector pAX50. Phage was prepared according to standard methods (see for example the prior art and applications filed by applicant cited herein) and stored after filter sterilization at 4° C. for further use.

US11858997B2. Amino acid sequences that modulate the interaction between cells of the immune system (2024-01-02). Ablynx N.V. [BE]. Inventors: Guy Hermans [BE], Peter Verheesen [BE], Edward Dolk [NL], Hendricus Renerus Jacobus Mattheus Hoogenboom [NL], Michael John Scott Saunders [BE], Hans De Haard [NL], Renee de Bruin [NL].
Full text: Click here
Patent 2024
Bacteriophages BLOOD Cloning Vectors DNA Library Ficoll Genes Hypaque Llamas PBMC Peripheral Blood Mononuclear Cells Reverse Transcriptase Polymerase Chain Reaction Sterilization
5
Llama Nanobody Encoding Protocol
Not available on PMC !

EXAMPLE 18

Peripheral blood mononuclear cells were prepared from blood samples obtained from llama No. 149 using Ficoll-Hypaque according to the manufacturer's instructions. Next, total RNA extracted was extracted from these cells and used as starting material for RT-PCR to amplify Nanobody encoding gene fragments. These fragments were cloned into phagemid vector pAX50. Phage was prepared according to standard methods (see for example the prior art and applications filed by applicant cited herein) and stored after filter sterilization at 4° C. for further use.

US11858997B2. Amino acid sequences that modulate the interaction between cells of the immune system (2024-01-02). Ablynx N.V. [BE]. Inventors: Guy Hermans [BE], Peter Verheesen [BE], Edward Dolk [NL], Hendricus Renerus Jacobus Mattheus Hoogenboom [NL], Michael John Scott Saunders [BE], Hans De Haard [NL], Renee de Bruin [NL].
Full text: Click here
Patent 2024
Bacteriophages BLOOD Cloning Vectors DNA Library Ficoll Genes Hypaque Llamas PBMC Peripheral Blood Mononuclear Cells Reverse Transcriptase Polymerase Chain Reaction Sterilization

Top products related to «Hypaque»

1
Ficoll hypaque by GE Healthcare
Sourced in United States, Sweden, United Kingdom, Germany, China, Norway, Spain, Canada, Italy, Austria
Ficoll-Hypaque is a density gradient medium used for the isolation of mononuclear cells from whole blood, bone marrow, or other tissues. It is a sterile, pyrogen-free solution consisting of Ficoll polymer and sodium diatrizoate.
2
Ficoll hypaque by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, Macao, Sao Tome and Principe
Ficoll-Hypaque is a density gradient medium used for the separation of cells, such as lymphocytes, from whole blood or other biological samples. It enables the isolation of specific cell types based on their density differences. The product provides a consistent and reliable method for cell separation, which is a crucial step in various applications, including immunology, cell biology, and diagnostics.
3
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
4
Rpmi 1640 medium by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Japan, France, Canada, Australia, Italy, Switzerland, Belgium, New Zealand, Spain, Israel, Sweden, Denmark, Macao, Brazil, Ireland, India, Austria, Netherlands, Holy See (Vatican City State), Poland, Norway, Cameroon, Hong Kong, Morocco, Singapore, Thailand, Argentina, Taiwan, Province of China, Palestine, State of, Finland, Colombia, United Arab Emirates
RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
5
Ficoll hypaque by Cytiva
Sourced in Sweden, United States, United Kingdom, Germany, Italy
Ficoll-Hypaque is a density gradient medium used for the separation and isolation of cells and cellular components. It is composed of a mixture of Ficoll, a hydrophilic polysaccharide, and sodium diatrizoate, a high-density compound. This mixture creates a density gradient that allows the separation of different cell types and subcellular fractions based on their density during centrifugation.
6
Rpmi 1640 by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Canada, Japan, Australia, Italy, Switzerland, Belgium, New Zealand, Austria, Netherlands, Israel, Sweden, Denmark, India, Ireland, Spain, Brazil, Norway, Argentina, Macao, Poland, Holy See (Vatican City State), Mexico, Hong Kong, Portugal, Cameroon
RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.
7
Streptomycin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Australia, Japan, Switzerland, Italy, Belgium, Israel, Austria, Spain, Netherlands, Poland, Brazil, Denmark, Argentina, Sweden, New Zealand, Ireland, India, Gabon, Macao, Portugal, Czechia, Singapore, Norway, Thailand, Uruguay, Moldova, Republic of, Finland, Panama
Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
8
Penicillin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Japan, Australia, Switzerland, Italy, Israel, Belgium, Austria, Spain, Brazil, Netherlands, Gabon, Denmark, Poland, Ireland, New Zealand, Sweden, Argentina, India, Macao, Uruguay, Portugal, Holy See (Vatican City State), Czechia, Singapore, Panama, Thailand, Moldova, Republic of, Finland, Morocco
Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
9
L glutamine by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, France, Canada, Switzerland, Italy, Australia, Belgium, China, Japan, Austria, Spain, Brazil, Israel, Sweden, Ireland, Netherlands, Gabon, Macao, New Zealand, Holy See (Vatican City State), Portugal, Poland, Argentina, Colombia, India, Denmark, Singapore, Panama, Finland, Cameroon
L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
10
Ficoll hypaque gradient by GE Healthcare
Sourced in United States, Sweden, United Kingdom, Austria, Germany
Ficoll-Hypaque gradient is a laboratory reagent used for the separation and isolation of specific cell types from a complex mixture, such as blood or other biological samples. It is a density gradient medium that allows the separation of cells based on their density.

More about "Hypaque"

Hypaque is a non-ionic, water-soluble iodinated contrast medium commonly used in radiographic imaging procedures such as angiography, urography, and other diagnostic tests.
It is known for its superior contrast and reduced side effects compared to older ionic contrast agents.
Researchers can utilize PubCompare.ai's AI-powered platform to streamline their Hypaque-related research, easily locating relevant protocols from literature, preprints, and patents, while leveraging automated comparisons to identify the most optimal protocols and products.
This innovative solution helps researchers optimize their Hypaque studies and advance their work more efficiently.
Ficoll-Hypaque, a density gradient medium, is often used in combination with Hypaque for cell separation and purification.
The Ficoll-Hypaque gradient is a popular technique for isolating mononuclear cells from whole blood or other biological samples.
RPMI 1640 medium, a commonly used cell culture medium, is also frequently used in conjunction with Hypaque and Ficoll-Hypaque for maintaining cell viability and growth.
In addition to Hypaque, researchers may also utilize other common reagents and supplements, such as fetal bovine serum (FBS), streptomycin, penicillin, and L-glutamine, to support their cell culture and experimental setups.
These components help to provide the necessary nutrients, antibiotics, and growth factors for optimal cell performance and survival.
By leveraging the insights and capabilities of PubCompare.ai's platform, researchers can streamline their Hypaque-related research, quickly identify the most relevant protocols and products, and optimize their studies for greater efficiency and success.