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Hypnorm

Hypnorm is a potent anesthetic agent that combines the analgesic properties of fentanyl, a powerful opioid, with the sedative and muscle-relaxing effects of fluanisone, a butyrophenone derivative.
This combination produces a profound state of deep anesthesia and analgesia, making it a valuable tool in veterinary and medical procedures requiring general anesthesia.
Hypnorm is commonly used in animal research protocols, particularly in studies involving pain management and surgical interventions.
Researchers can leverage the power of AI-driven optimization with PubCompare.ai to easily locate and compare Hypnorm-related protocols from literature, pre-prints, and patents, empowering them to identify the best protocols and products for their specific research needs.
By streamlining the research process and unlocking new insights, PubCompare.ai helps researchers maximize the efficiency and effectiveness of their Hypnorm-based studies.

Most cited protocols related to «Hypnorm»

Six weeks old female NMRI (Naval Medical Research Institute) nude mice were acquired from Taconic Europe (Lille Skensved, Denmark) and allowed to acclimate one week in the animal facility before any intervention was initiated. All experimental procedures were conducted with the guidelines set forth by the Danish Ministry of Justice. Estrogen pellets, 0.72 mg 17-β-Estradiol, 60-day release (Innovative Research of America, Sarasota, FL, USA), were implanted s.c. during anesthesia with 1:1 v/v mixture of Hypnorm® (Janssen Pharmaceutica, Beerse, Belgium) and Dormicum® (Roche, Basel, Switzerland). One week after implantation of pellets, MCF-7 (human breast adenocarcinoma) tumor cells (107 cells in 100 μL medium mixed with 100 μL Matrixgel™ Basement Membrane Matrix (BD Biosciences, San Jose, CA, USA)) were injected subcutaneous into the left and right flank respectively. Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) medium supplemented with 10% fetal calf serum and 1% penicillin-streptomycin in 5% CO2 at 37°C.
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Publication 2008
Adenocarcinoma Anesthesia Animals Breast Cells Culture Media Dormicum Eagle Estradiol Estrogens Females Fetal Bovine Serum Homo sapiens Hypnorm Magnetic Resonance Imaging Membrane, Basement Mice, Nude Neoplasms Ovum Implantation Pellets, Drug Penicillins Streptomycin
Adult, female 200–250 g Wistar rats (n=4 eyes per treatment) were anaesthetized intraperitoneally with Hypnorm/Hypnovel anaesthetic (Janssen Pharmaceuticals, Oxford, UK) and the ON of both eyes were crushed (ONC) intraorbitally to completely transect all RGC axons leaving sheath and artery intact. All reagents were intravitreally injected using glass micropipettes in a final volume of 10 μl. Animals were treated with either PBS or with 20 μg control siCNL or with 5, 10 or 20 μg siCASP2 or with 5 μg BDNF (Peprotech Ltd, London, UK) immediately after ONC and within 5 min post-surgery.
At 5 days post-ONC, 2 μl of 4% FG (Cambridge Bioscience, Cambridge, UK) retrograde tracer was injected into the nerve mid-way between the lamina cribrosa and the site of ONC. After 48 h, animals were killed by exposure to CO2 and intracardially perfused with 4% formaldehyde (TAAB Laboratories, Aldermaston, UK). Retinas were dissected out and immersion fixed in 4% formaldehyde (TAAB Laboratories) for 30 min and whole-mounted onto glass slides (VWR International, Lutterworth, UK). Four equidistal radial cuts were made to give four equally sized quadrants, attached together around the optic disc. Retinal whole mounts were air dried and mounted in Vectashield mounting medium (Vector Laboratories, Peterborough, UK). An observer was blinded to the treatments and groups and samples were randomly assigned numbers before image capture and analysis. Images were captured at × 20 magnification using a Zeiss epifluorescent microscope (Zeiss, Hertfordshire, UK) equipped with a Axiocam HRc camera (Zeiss) running the Axiovision software (Zeiss). Images were captured from three different areas of each quadrant (total=12 counts per quadrant, n=4 retinas per treatment, to account for variation of RGC numbers in the different areas and quantified using the built-in particle counting facilities in ImagePro; Media Cybernetics, Bethesda, MD, USA) and expressed as the number of RGC per mm2±S.E.M.
Publication 2011
Administration, Ophthalmic Adult Anesthetics Animals Arteries Axon Cloning Vectors Eye Formaldehyde Hypnorm Microscopy Nervousness Operative Surgical Procedures Optic Disk Pharmaceutical Preparations Plates, Cribriform Rats, Wistar Retina Submersion Woman
Studies were conducted with 197 male and female homozygous nude rats (Han:rnu/rnu Rowett) bred and maintained in an isolation facility in a pathogen free environment on a standard 12/12 h day and night cycle. Animals were fed a standard sterilised pellet diet and provided sterile tap water ad libitum. The athymic nude rat is T-cell deficient, but has normal complement and B-cell function [11 (link)]. 10 tumour spheroids (250-350 μm in diameter) were selected under a light microscope. The animals were anaesthetized with Hypnorm-Dormicum (0.4 ml/kg) s.c., the head secured in a stereotactic frame (Benchmark; Neurolab, St Louis, MO) and a short longitudinal incision was made in the scalp exposing the calvarium. A burr-hole was made 1 mm posterior to the bregma and 3 mm to the right of the sagittal suture using a micro-drill with a bit diameter of 2,9 mm. A Hamilton syringe with inner diameter of 810 μm was introduced to a depth of 2, 5 mm below the brain surface, and the spheroids were slowly injected and the syringe left in place for 3 min before withdrawal. The skin was closed with an Ethilon 3-0 suture. The tumours were allowed to grow for 4-5 months, then harvested and passaged onto new animals after initiation of spheroids in vitro. Animals were sacrificed at the onset of symptoms using CO2 and the brains were removed. All procedures and experiments involving animals in this study were approved by The National Animal Research Authority and conducted according to the European Convention for the Protection of Vertebrates Used for Scientific purposes.
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Publication 2009
Animals B-Lymphocytes Brain Calvaria Conferences Diet Dormicum Drill Ethilon Europeans Females Head Homozygote Hypnorm isolation Light Microscopy Males Mice, Nude Neoplasms Pathogenicity Physiology, Cell Rats, Nude Reading Frames Scalp Skin Sterility, Reproductive Sutures Syringes T-Lymphocyte Trephining Vertebrates
Upon termination, the mice were anesthetized by subcutaneous injection of Hypnorm/Dormicum mixture diluted in sterile water 1:2 (Hypnorm: fentanyl citrate 0.75 mg/kg and fluanisone 23.8 mg/kg, VetaPharma Leeds, UK; Dormicum: midazolam 11.9 mg/kg, Roche a/s, Hvidovre, Denmark). Heart blood was withdrawn. Lungs were flushed twice with sterile 0.9% NaCl through the trachea to obtain BAL fluid, the used volume was calculated as 1 ml 0.9% NaCl/25g mouse weight and varied from 0.7 to 0.9 ml. BAL fluid was stored on ice until centrifugation at 400g for 10 min at 4°C. The BAL cells were re-suspended in 100 µl medium (HAM F-12 with 1% penicillin/streptomycin and 10% fetal bovine serum). Acellular BAL fluid was recovered and stored at −80°C. The total number of living and dead cells in BAL was determined by NucleoCounter NC-200TM (Chemometec, Denmark) from diluted cell suspensions following manufacturer's protocol. The total cell counts were calculated for each mouse.
Samples for COMET assay were prepared from 40 µl re-suspended BAL cells and 60 µl freezing media (HAM F-12, 1% penicillin/streptomycin, 15% fetal bovine serum and 10% DMSO). Samples were divided into two aliquots and immediately frozen at −80°C. The rest of the cell re-suspension (40 µl) was used to estimate the number of granulocytes (neutrophil and eosinophil), macrophages, lymphocytes, and epithelial cells in BAL fluid. The cell suspension was centrifuged at 55g for 4 min in Cytofuge 2 (StatSpin, TRIOLAB, Brøndby, Denmark) and fixed for 5 min in 96% ethanol. All slides were stained with May-Günewald-Giemsa stain, randomized, and blinded before counting 200 cells/sample under light microscope with 100 × magnification (using immersion oil). The numbers of counted cells were expressed as % observations based on cell distribution of the 200 counted cells multiplied with the total number of cells for each mouse. Lung and liver tissue were divided into 4 parts, snap frozen in liquid nitrogen, and stored at −80°C.
Publication 2014
BLOOD Cells Centrifugation Comet Assay Dormicum Eosinophil Epithelial Cells Ethanol Fentanyl Citrate Fetal Bovine Serum fluanisone Freezing Granulocyte Heart Hypnorm Light Microscopy Liver Lung Lymphocyte Macrophage Midazolam Mus Neutrophil Nitrogen Normal Saline Penicillins Stain, Giemsa Stains Sterility, Reproductive Streptomycin Subcutaneous Injections Submersion Sulfoxide, Dimethyl Tissues Trachea
Confluent cells were imaged on 35-mm glass bottom dishes at 37°C and 5% CO2 using a Leica DMI 6000 SP8 confocal microscope or an Olympus FV1000 confocal microscope with a SIM scanner (Serrels et al., 2009 (link)). For photobleaching (FRAP, FLIP) experiments in live xenograft tumors, mice were anesthetized using a combination of 1:1 hypnorm - H2O + 1:1 hypnovel - H2O, and the subcutaneous tumor was exposed surgically via a skin flap procedure (Serrels et al., 2009 (link)) on a 37°C heated stage. Mice were sacrificed within 4 hr of imaging.
Detailed protocols can be found in the Supplemental Experimental Procedures.
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Publication 2015
Cells Hyperostosis, Diffuse Idiopathic Skeletal Hypnorm Microscopy, Confocal Mus Neoplasms Neoplasms, Experimental Operative Surgical Procedures Skin Surgical Flaps Xenografting

Most recents protocols related to «Hypnorm»

BN rats were anesthetised on Day 53 by injection of hypnorm-dormicum and initial ear thickness was measured twice. Subsequently, an EST was performed by intradermally injecting 10 µg of PPE in 20 µL PBS into right ear of each rat as previously described (34 (link)). Ear thickness was measured again 30 min after the injection and ear swelling was determined as a measure of the clinical relevance of the peanut sensitisation.
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Publication 2023
Arachis hypogaea Dormicum Hypnorm Rats, Inbred BN
For Insulin Tolerance Test, non-starved mice were injected intraperitoneally with insulin (Actrapid Penfill) (1 U/kg body weight). Intraperitoneal Glucose Tolerance Test (GTT) combined with Glucose Stimulated Insulin Secretion (GSIS) were performed on 6 h fasted and sedated mice (Hypnorm (Veta Pharma)/Midazolam (Hamlenmice)) following i.p. administration of glucose (SIGMA #G7021) (1 g/kg body weight). Blood glucose and plasma insulin levels were analysed from blood samples taken at indicated time points for both procedures using a Glucometer (Ultra 2, One Touch) and the ultra-sensitive mouse insulin ELISA kit (Chrystal Chem Inc. #90080), respectively.
Blood profiling of liver function was performed using the Mammalian Liver Profile rotor on a VetScan VS2 (Abaxis, Inc.). Liver TG and cholesterol were measured using Serum Triglyceride Determination Kit (Sigma-Aldrich #TR0100) and Cholesterol / Cholesteryl Ester Quantitation Kit (MBL #JM-K603-100) according to manufacturer’s recommendations. Body composition of live mice was measured using EchoMRI (EchoMRI LLC; EchoMRI 3-in-1). Liver and tumour histology was assessed using haematoxylin and eosin (H&E) and picrosirius red in paraffin-embedded sections using standard procedures.
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Publication 2023
BLOOD Blood Glucose Blood Physiological Phenomena Body Composition Body Weight Cholesterol Cholesterol Esters Enzyme-Linked Immunosorbent Assay Eosin Glucose Glucose Tolerance Test Hematoxylin Hypnorm Immune Tolerance Insulin Insulin Secretion Liver Mammals Mice, House Midazolam Neoplasms Paraffin Embedding Plasma Serum Touch Triglycerides
Isolated perfused rat hearts were prepared as previously described [53 (link)]. In brief, rats were anesthetized by a subcutaneous injection of Hypnorm-Dormicum (fentanyl citrate, 0.158 mg/kg body weight; fluanisone, 0.5 mg/kg body weight; midazolam, 0.5 mg/kg body weight) and mechanically ventilated through a tracheostomy. A bolus of 500 IU heparin for anticoagulation was administered through the femoral vein. Then, the ascending aorta was cannulated via a thoracoabdominal incision and retrogradely perfused at a constant pressure of 80 mmHg with 37 °C KH buffer continuously aerated with 5% CO2/balance O2. Following transfer to the Langendorff system, a balloon was inserted in the left ventricle through the left atrial appendage and pressurized to 5–8 mmHg to simulate preload. After the hearts had stabilized in KH buffer for 45 minutes, we tested the influence of adding 3 or 10 mM racemic Na-3-OHB or NaCl. We report changes from baseline (measured 10 or 20 minutes after buffer change) for Na-3-OHB after subtraction of the response to equimolar NaCl. We excluded hearts that during stabilization did not fulfill the following inclusion criteria adjusted for rat size [14 (link)]: Coronary flow: 10–35 mL/min, arrhythmias: <10 ectopic beats and no sustained ventricular tachycardia or fibrillation, heart rate: 150–400 min–1, left ventricular systolic pressure: >120 mmHg.
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Publication 2023
Ascending Aorta Atrium, Left Auricular Appendage Body Weight Buffers Cardiac Arrhythmia Dormicum Fentanyl Citrate fluanisone Heart Heparin Hypnorm Left Ventricles Midazolam Premature Cardiac Complex Pressure Rate, Heart Sodium Chloride Subcutaneous Injections Systolic Pressure Tachycardia, Ventricular Tracheostomy Vein, Femoral
Animal procedures were followed the guidelines of the Institutional Ethics Review Board of Xijing Hospital. The rat IVDD models were constructed by needle puncturing, as previously described [12 (link)].
To investigate the impact of miR-27a-3p on macrophages M1 polarization and IVDD progression in vivo, we extracted NPc from Sprague-Dawley (SD) rats and regulated the expression of miR-27a-3p using mimic or inhibitor. The exosomes of the manipulated NPc were then collected. Then, SD rats (200–250 g) were used to construct IVDD model. In brief, Hypnorm and Dormicum were used to anesthetize the SD rats. The caudal spine was identified by palpation. A longitudinal incision was made in the rat tail skin, and the muscle tissue and subcutaneous connective tissue were separated. Next, an 18-gauge sterile needle was inserted into the disc center to a 5 mm depth, rotated 360 degrees and held for 30 s. Then, a total of 5µL rat-miR-27a-3p-mimic or rat-miR-27a-3p-inhibitor was injected into the rat model via a 27G needle. We monitored the rats daily after surgery to ensure their health condition. After 4 weeks, the rats were put to death and IVDs were obtained from different groups for further experiments. The degenerative control (DC) group consisted of rats treated with PBS after puncture surgery, while the normal control (NC) group consisted of rats that underwent no surgery. A total of 30 SD rats were used for the animal experiment. The primer sequences of miR-27-3p mimic or inhibitor can be found in Additional Table 1.
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Publication 2023
Animals ARID1A protein, human Disease Progression Dormicum Exosomes Hypnorm Macrophage Activation Muscle Tissue Needles Oligonucleotide Primers Operative Surgical Procedures Palpation Punctures Rats, Sprague-Dawley Rattus norvegicus Skin Sterility, Reproductive Subcutaneous Tissue Tail Vertebral Column

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Publication 2023
Anesthesia Animals Asepsis Blink Reflexes Cochlea Dental Health Services Diazepam Drill Fenestra Cochleae Fentanyl Citrate Fibrosis fluanisone Foot Hypnorm Intramuscular Injection Lidocaine Hydrochloride Muscle Tissue Nylons Operative Surgical Procedures Ovum Implantation Povidone Iodine Silicones Sulfate, Atropine Sutures

Top products related to «Hypnorm»

Sourced in Belgium, United Kingdom
Hypnorm is a laboratory equipment product manufactured by Johnson & Johnson. It is designed to provide a stable and controlled environment for conducting various experiments and research activities.
Sourced in United Kingdom, Switzerland
Hypnovel is a sedative-hypnotic medication manufactured by Roche. It contains the active ingredient midazolam, which is a benzodiazepine drug used for the induction and maintenance of general anesthesia, as well as for the treatment of acute agitation and anxiety. Hypnovel is administered intravenously and has a rapid onset of action.
Sourced in Switzerland, Belgium, United Kingdom, Germany, France, Japan, Sweden, United States, Turkiye
Dormicum is a drug used as a sedative and anesthetic in various medical procedures. It contains the active ingredient midazolam, which is a benzodiazepine medication. Dormicum is administered intravenously or intramuscularly by healthcare professionals to induce sedation or anesthesia.
Sourced in United States, United Kingdom, Germany, Denmark, Switzerland, Italy, Australia
Rimadyl is a veterinary pharmaceutical product manufactured by Pfizer. It is a non-steroidal anti-inflammatory drug (NSAID) used to reduce inflammation and pain in dogs and cats. The active ingredient in Rimadyl is carprofen.
Sourced in United States, Germany, Italy
The Stereotaxic frame is a laboratory instrument used to immobilize and position the head of a subject, typically an animal, during surgical or experimental procedures. It provides a secure and reproducible method for aligning the subject's head in a three-dimensional coordinate system to enable precise targeting of specific brain regions.
Sourced in Switzerland, Germany, Denmark, United States, United Kingdom, Belgium, Finland
Midazolam is a benzodiazepine compound used as a sedative and hypnotic in medical and clinical settings. It is commonly used for the induction and maintenance of anesthesia, as well as for the management of certain types of seizures. Midazolam primarily functions as a short-acting central nervous system depressant, exhibiting anxiolytic, amnestic, hypnotic, anticonvulsant, and muscle relaxant properties.
Sourced in Germany
Midazolam is a type of laboratory equipment used for medical purposes. It is a benzodiazepine drug that acts as a sedative and muscle relaxant. Midazolam is commonly used as a pre-medication before medical procedures or to induce anesthesia.
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PClamp 9 software is a comprehensive data acquisition and analysis software designed for electrophysiology research. It provides a suite of tools for recording, visualizing, and analyzing electrical signals from cells and tissues.
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The Mouse Insulin ELISA is a laboratory assay used to measure insulin levels in mouse samples. It is a quantitative enzyme-linked immunosorbent assay (ELISA) designed to determine the concentration of insulin in mouse serum, plasma, or other biological samples.
Sourced in United States, Denmark
BALB/c mice are a widely used inbred mouse strain. They are a common laboratory animal model known for their susceptibility to certain diseases and their use in immunological research.

More about "Hypnorm"

Hypnorm, a powerful anesthetic agent, combines the analgesic properties of the opioid fentanyl with the sedative and muscle-relaxing effects of the butyrophenone derivative fluanisone.
This potent combination induces a profound state of deep anesthesia and analgesia, making it a valuable tool in veterinary and medical procedures requiring general anesthesia.
Hypnorm is widely used in animal research protocols, particularly in studies involving pain management and surgical interventions.
Researchers can leverage the power of AI-driven optimization with PubCompare.ai to easily locate and compare Hypnorm-related protocols from literature, pre-prints, and patents.
This empowers them to identify the best protocols and products for their specific research needs.
By streamlining the research process and unlocking new insights, PubCompare.ai helps researchers maximize the efficiency and effectiveness of their Hypnorm-based studies.
Hypnovel, Dormicum, and Midazolam are benzodiazepine sedatives that may be used in conjunction with or as alternatives to Hypnorm in various research and clinical settings.
Rimadyl, a non-steroidal anti-inflammatory drug (NSAID), is often used to manage pain and inflammation in animal research studies.
Stereotaxic frames are essential tools used in neuroscience research, including studies involving Hypnorm anesthesia.
The PClamp 9 software is a popular tool for electrophysiological data acquisition and analysis, which can be utilized in Hypnorm-based research protocols.
Furthermore, the Mouse Insulin ELISA is a common assay used to measure insulin levels in Hypnorm-anesthetized BALB/c mice, a widely used mouse strain in biomedical research.
By incorporating these related terms and concepts, researchers can optimize their Hypnorm-based studies and unlock new discoveries.