BRafCA, Tyr::CreER and Ptenlox4-5 mice were genotyped as previously described 17 (link),20 (link),24 (link). Cre-mediated conversion of BRafCA to BRafVE and the deletion of exons 4 and 5 of Pten were assessed by PCR as previously described. Topical administration of 4-hydroxytamoxifen (4-HT) was performed by preparing a 25-50mg/ml (65-130mM) solution of 4-HT (70% Z-isomer, Sigma) in DMSO and applying enough solution to wet the right ear, right flank and tail with a small paint brush on post-natal days 2, 3, and 4. For localized melanoma induction on the back skin, adult (6-8 weeks of age) mice were treated topically with 1-2 μl of 1.9mg/ml (5mM) 4-HT at 6-8 weeks of age using a similar protocol. Generalized induction in adult mice was performed by intra-peritoneal injection of 1mg of tamoxifen/40g mouse on 3 consecutive days. In this case tamoxifen was prepared as a 10mg/ml suspension in peanut oil. PD352901 was dissolved in 0.5%(w/v) Hydroxy-propyl-methylcellulose, 0.2%(v/v) Tween 80 (Sigma) and administered to mice daily by oral gavage at a dose of 12.5mg/kg. Rapamycin (LC Laboratories, Woburn, MA) was suspended in 0.5%(w/v) methylcellulose and administered to mice daily by oral gavage at a dose of 7.5mg/kg. Control animals in the melanoma prevention studies were administered with the relevant solvent. Tissues were prepared for analysis as previously described 17 (link),20 (link)
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Hypromellose
Hypromellose
Hypromellose, also known as hydroxypropyl methylcellulose, is a semisynthetic, partially substituted cellulose ether used as a pharmaceutical excipient and viscosity-increasing agent.
It is commonly employed in formulations for controlled drug release, ocular delivery, and topical applications.
PubCompare.ai's AI-driven platform helps researchers identify the most repoducible and accurate hypromellose research methods from literature, preprints, and patents, empowering reliable studies.
Explore this seamless solution to optimize your hypromellose-related research needs.
It is commonly employed in formulations for controlled drug release, ocular delivery, and topical applications.
PubCompare.ai's AI-driven platform helps researchers identify the most repoducible and accurate hypromellose research methods from literature, preprints, and patents, empowering reliable studies.
Explore this seamless solution to optimize your hypromellose-related research needs.
Most cited protocols related to «Hypromellose»
Administration, Topical
Adult
Animals
Deletion Mutation
Exons
Familial Atypical Mole-Malignant Melanoma Syndrome
hydroxytamoxifen
Hypromellose
Injections, Intraperitoneal
Isomerism
Melanoma
Methylcellulose
Mice, House
Peanut Oil
PTEN protein, human
Sirolimus
Solvents
Sulfoxide, Dimethyl
Tail
Tamoxifen
Tissues
Tube Feeding
Tween 80
Antidepressive Agents
beta-Arrestin 2
Brain
Corticosterone
Cortisone
Cyclodextrins
Fluoxetine
Fluoxetine Hydrochloride
Hypromellose
Imipramine Hydrochloride
Light
Mice, House
Reboxetine
Tube Feeding
Animals
Cells
Common Cold
Cyclodextrins
Doxycycline
Food
Hypromellose
Leukemia
Mice, House
RNA Interference
Short Hairpin RNA
Sucrose
Sulfoxide, Dimethyl
Transplantation
Drugs were administered according to a randomised latin-square design. CNO (Enzo Lifesciences, NY, USA) and CLZ (Sigma Aldrich, UK) were dissolved in hydrochloric acid (1:15) and 40% hydroxypropyl-β-cyclodextrine (1:10). The pH was adjusted to 6.5–7.5. Cmpd-21 (HelloBio, UK) was dissolved in 0.9% saline. The dose range and pre-treatment time were selected based on in-house pharmacokinetic data and published literature32 (link). Since a fully automated data acquisition system was used, the experimenter was not blinded to treatment. All drugs were administered i.p. at a volume of 10 ml/kg 10 min before the start of the behavioural test. The applied doses were 3.5 or 10 mg/kg for CNO, 0.1, 0.3 or 1 mg/kg for CLZ, and 0.3, 1 or 3 mg/kg for Cmpd-21, in addition to vehicle in each case. Mice were habituated to injections by i.p. administration of 0.9% saline 3–5 days before drug administration.
Behavior Test
Dysplasia, Campomelic
Hydrochloric acid
Hypromellose
Mice, House
Normal Saline
Pharmaceutical Preparations
6,7-dinitroquinoxaline-2,3-dione
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Amino Acids
Bicarbonate, Sodium
Cardiac Arrest
cesium chloride
CGP 55845
CGP55845
Diploid Cell
Dopamine D2 Receptor
GABA-A Receptor
Glucose
HEPES
Hypromellose
Induced Pluripotent Stem Cells
Inhibitory Postsynaptic Currents
isoguvacine
isolation
Kainate
Kinetics
Magnesium Chloride
Medical Devices
N-Methylaspartate
Pharmaceutical Preparations
Phosphinic Acids
QX-314
Seizures
Sodium Chloride
Sulfate, Magnesium
Sulpiride
Tetrodotoxin
Most recents protocols related to «Hypromellose»
Example 4
Composition H was manufactured according to the following procedure:
- a) Specified amount of purified water was taken in a suitable container and specified quantity of docusate sodium was added and stirred continuously to obtain a solution.
- b) Sodium lauryl sulphate was added to the step (a) solution and stirred continuously to obtain a solution.
- c) Hydroxypropyl methyl cellulose was added to the step (b) solution and stirred continuously to obtain a solution.
- d) Mifepristone was added to the step (c) solution and stirred for 5 minutes to obtain Mifepristone dispersion.
- e) Mifepristone dispersion was homogenized using IKA's Ultra TURRAX® homogenizer at 1000 RPM for 15 minutes.
- f) The above homogenized mifepristone slurry was nano-sized in ball-mill chamber to obtain nano-suspension containing desired particle size of mifepristone. The particle size distribution was measured by using Mastersizer 3000 particle analyser.
- g) Specified quantities of the silicified microcrystalline cellulose and sodium starch glycolate were dispensed in a bowl and warmed to reach 28° C. to 30° C. temperature.
- h) The nano-sized mifepristone suspension according to step (f) was sprayed onto the warmed intra-granular material according to step (g). The sprayed granules were dried at a temperature of 50° C. to 65° C. and sieved through 30 number mesh sieve.
- i) Specified quantities of milled granules of step (h), sodium starch glycolate, microcrystalline cellulose, colloidal silicon dioxide and magnesium stearate were blended and compressed using tablet compression machine. The tablets according to step (i) were coated with suitable coating materials.
Cytoplasmic Granules
Docusate Sodium
Hypromellose
magnesium stearate
microcrystalline cellulose
Mifepristone
Pharmaceutical Preparations
Silicon
Silicon Dioxide
sodium starch glycolate
Sulfate, Sodium Dodecyl
Example 3
The pulverized vicagrel salt was placed with pregelatinized starch, lactose, and sodium carboxymethyl starch in a fluidized bed, fluidized mixing is started for 10 min, and 5% hydroxypropyl methylcellulose is prepared as a binder. At an air inlet temperature of 80° C., the binder was sprayed while maintaining a bed temperature at 40-50° C. The formed particles were dried for 30 min while the bed temperature was maintained at 50-60° C., and were discharged. Sodium stearyl fumarate was added and mixed for 5 min, and the particles were filled into capsules.
Capsule
carboxymethyl starch
Fumarate
Hypromellose
Lactose
sodium carboxymethyl starch
Sodium Chloride
sodium stearyl fumarate
Starch
vicagrel
Example 133
Step 1: tert-butyl (2S)-2-(3-cyano-3-hydroxypropyl)pyrrolidine-1-carboxylate. To a solution of tert-butyl (S)-2-(3-oxopropyl)pyrrolidine-1-carboxylate (114 mg, 0.5 mmol) in DCM (1 mL) was added Et3N (0.1 mL) and acetone cyanohydrin (0.1 mL, 1.2 mmol). The mixture was stirred at r.t. over night. The solution was concentrated by vacuum and purified by silica gel column to afford the title product (120 mg) as colorless oil. MS: [M+1]+ 255.
Step 2: 2-hydroxy-4-((S)-pyrrolidin-2-yl)butanoic acid. To a solution of tert-butyl (2S)-2-(3-cyano-3-hydroxypropyl)pyrrolidine-1-carboxylate (60 mg, 0.23 mmol) in dioxane (0.8 mL) was added HCl (con., 0.8 mL). The mixture was stirred at 100° C. overnight. After cooling to r.t., dioxane was removed by evaporation. The residue was washed by EtOAc (0.5 mL×2). The aqueous phase was collected and evaporated to give a crude product which was used directly in the next step without purification. MS: [M+1]+ 174.
Step 3: 4-((S)-1-(7,8-dichloro-4-(1H-imidazol-1-yl)quinolin-2-yl)pyrrolidin-2-yl)-2-hydroxybutanoic acid (I-817). The title compound was prepared essentially by the same methods as for I-664. MS: [M+1]+ 435.
2-hydroxybutyric acid
acetone cyanohydrin
Anabolism
Butyric Acid
Dioxanes
Hypromellose
imidazole
pyrrolidine
Silica Gel
TERT protein, human
Vacuum
Example 21
To a solution of (R)-5-(tert-butyl)-N-(8-(2-((1-methyl-1H-pyrazol-4-yl)amino)pyrimidin-4-yl)-2,3,4,5-tetrahydro-1H-benzo[c]azepin-5-yl)-1,3,4-oxadiazole-2-carboxamide (101 mg, 0.21 mmol) in MeOH (7 mL) was added (S)-2-methyloxirane (29 μL, 0.42 mmol) and cesium carbonate (135 mg, 0.42 mmol). The mixture was stirred at 60° C. for 16 h. The reaction mixture was cooled to room temperature and filtered. The filtrate was concentrated and the crude product was purified by silica gel chromatography (DCM:MeOH=10:1) to give 5-(tert-butyl)-N—((R)-2-((S)-2-hydroxypropyl)-8-(2-((1-methyl-1H-pyrazol-4-yl)amino)pyrimidin-4-yl)-2,3,4,5-tetrahydro-1H-benzo[c]azepin-5-yl)-1,3,4-oxadiazole-2-carboxamide as a yellow solid (50 mg, yield: 44%). ESI-MS (M+H)+: 546.0. 1H NMR (400 MHz, METHANOL-d4) δ: 8.41 (d, J=5.3 Hz, 1H), 8.07-8.02 (m, 2H), 7.97 (s, 1H), 7.63 (s, 1H), 7.48 (d, J=8.5 Hz, 1H), 7.22 (d, J=5.3 Hz, 1H), 5.62-5.53 (m, 1H), 4.33-4.10 (m, 2H), 4.04-3.95 (m, 1H), 3.89 (s, 3H), 3.33-3.27 (m, 2H), 2.58-2.46 (m, 2H), 2.38-2.23 (m, 1H), 2.07-1.95 (m, 1H), 1.48 (s, 9H), 1.15 (d, J=6.3 Hz, 3H).
1H NMR
cesium carbonate
Chromatography
compound 21
Gel Chromatography
Hypromellose
Methanol
Oxadiazoles
propylene oxide
pyrazole
Silica Gel
Silicon Dioxide
TERT protein, human
Example 124
Step 1: tert-Butyl (S)-2-(2-oxoethyl)pyrrolidine-1-carboxylate. To a solution of tert-butyl (S)-2-(2-hydroxyethyl)pyrrolidine-1-carboxylate (340 mg, 1.5 mmol) in DCM (6 mL) and pyridine (1 mL) was added DMP (800 mg) at 0° C. The mixture was stirred at r.t. for 2 h and quenched by Na2S2O3/NaHCO3 (sat., 10 mL). The organic phase was concentrated by vacuum and purified by silica gel chromatography to afford the title product (280 mg) as colorless oil. MS: [M+1]+ 214.
Step 2 and step 3 were essentially the same procedure as described above to prepare tert-butyl (S)-2-(3-hydroxypropyl)pyrrolidine-1-carboxylate preparation (Step 1 and Step 2).
Anabolism
Bicarbonate, Sodium
Chromatography
Gel Chromatography
Hypromellose
pyridine
pyrrolidine
Silica Gel
Silicon Dioxide
TERT protein, human
Vacuum
Top products related to «Hypromellose»
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2-hydroxypropyl-β-cyclodextrin is a modified form of the naturally occurring cyclodextrin molecule. It is a cyclic oligosaccharide composed of seven glucose units. The hydroxypropyl group is used to increase the solubility and stability of the cyclodextrin.
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Hydroxypropyl methylcellulose is a water-soluble, non-ionic cellulose ether that is commonly used as a thickening agent, stabilizer, and binder in various industrial and pharmaceutical applications. It is a versatile material that can be used to modify the physical and rheological properties of aqueous solutions, suspensions, and gels.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Tween 80 is a non-ionic surfactant and emulsifier. It is a viscous, yellow liquid that is commonly used in laboratory settings to solubilize and stabilize various compounds and formulations.
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Hydroxypropyl-β-cyclodextrin is a modified form of the naturally occurring cyclodextrin compound. It is a white, water-soluble powder commonly used as an excipient in pharmaceutical and biotechnology applications. Hydroxypropyl-β-cyclodextrin is capable of forming inclusion complexes with a variety of guest molecules, which can help improve their solubility, stability, and bioavailability.
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Hydroxypropyl methylcellulose (HPMC) is a water-soluble, semi-synthetic, and non-ionic cellulose ether. It is commonly used as a binder, thickening agent, and film-forming material in various pharmaceutical, cosmetic, and food applications.
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Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.
More about "Hypromellose"
Hypromellose, also known as hydroxypropyl methylcellulose (HPMC), is a semisynthetic, partially substituted cellulose ether used as a pharmaceutical excipient and viscosity-increasing agent.
It is commonly employed in formulations for controlled drug release, ocular delivery, and topical applications.
Closely related to hypromellose are other cellulose derivatives like 2-hydroxypropyl-β-cyclodextrin and hydroxypropyl-β-cyclodextrin, which have similar applications in drug delivery and formulation.
These compounds can be used in conjunction with hypromellose to enhance solubility, stability, and bioavailability of active pharmaceutical ingredients (APIs).
Hypromellose research often involves the use of various solvents and reagents, such as DMSO, Tween 80, acetonitrile, sodium hydroxide, methanol, and ethanol.
These substances can be utilized to prepare, characterize, and evaluate hypromellose-based formulations and their performance.
PubCompare.ai's AI-driven platform helps researchers identify the most reproducible and accurate hypromellose research methods from literature, preprints, and patents, empowering reliable studies.
This seamless solution optimizes the research process, allowing scientists to explore and compare hypromellose-related protocols with ease and confidence.
Leveraging the insights gained, researchers can conduct high-quality studies that advance the understanding and applications of this versatile pharmaceutical excipient.
It is commonly employed in formulations for controlled drug release, ocular delivery, and topical applications.
Closely related to hypromellose are other cellulose derivatives like 2-hydroxypropyl-β-cyclodextrin and hydroxypropyl-β-cyclodextrin, which have similar applications in drug delivery and formulation.
These compounds can be used in conjunction with hypromellose to enhance solubility, stability, and bioavailability of active pharmaceutical ingredients (APIs).
Hypromellose research often involves the use of various solvents and reagents, such as DMSO, Tween 80, acetonitrile, sodium hydroxide, methanol, and ethanol.
These substances can be utilized to prepare, characterize, and evaluate hypromellose-based formulations and their performance.
PubCompare.ai's AI-driven platform helps researchers identify the most reproducible and accurate hypromellose research methods from literature, preprints, and patents, empowering reliable studies.
This seamless solution optimizes the research process, allowing scientists to explore and compare hypromellose-related protocols with ease and confidence.
Leveraging the insights gained, researchers can conduct high-quality studies that advance the understanding and applications of this versatile pharmaceutical excipient.