Hawaiian CB4856 males were crossed into EG1000 dpy-5(e61) I; rol-6(e187) II; lon-1(e1820) III. Fifty Dpy animals and fifty non-Dpy animals from among the self-progeny of EG1000/CB4856 heterozygote hermaphrodites were picked into separate tubes, each containing 20 μL single-worm lysis buffer (50 mM KCl, 10 mM Tris pH 8.3, 2.5 mM MgCl2, 0.45% IGEPAL CA-630, 0.45% Tween 20, 0.01% (w/v) gelatin, 60 ug/ml proteinase K). A further 96 Dpy animals were picked to individual plates for use in interval mapping (see below). They were lysed by freezing at -80°C followed by incubation at 65°C 1 hour and proteinase was inactivated by incubation at 95°C 15 minutes. The Dpy lysate DNA templates were then added to a PCR master mix containing 424 μL water, 52 μL 10X PCR buffer (10X: 22.5 mM MgCl2, 500 mM Tris-HCl, 140 mM (NH4)2SO4, pH 9.2 at 25°C), 10.4 μL 10 mM dNTPs, and 3.12 μL Taq (5 units/μl). A similar mix was made with the 50 non-Mutant animals. 9.8 μL of the mutant mix or the non-mutant mix was aliquoted into alternate rows of a 96-well PCR plate (Figure 1A ). Primer pairs were arrayed into a microtiter plate at 10 μM each primer, so that neighboring rows contain duplicate pairs, and pin-replicated into the master mix. PCR reactions were done using the cycling conditions: 2' 94°C, 35 cycles of (15" 94°C, 45" 60°C, 1' 72°C), 5' 72°C. After amplification, PCR products were digested in the plate with the restriction enzyme DraI in a final volume of 16 μL (10 μL PCR product, 4.15 μL H2O 1.6 μL 10X DraI buffer (New England Biolabs), 0.25 μL DraI (10 units/μL, New England Biolabs)). This was accomplished by adding 6 μL of the enzyme plus enzyme buffer mix to each well using a multi-channel pipette followed by brief centrifugation in a Sorval RT6000D centrifuge with an H1000B rotor. Digestion reactions were incubated at 37°C at least 4 hours. Samples were then loaded onto a 2.5% agarose gel using an 8-channel pipette. The resulting gel displays all 48 SNP markers, from left to right and from chromosome I to X. Each Mutant SNP is next to its non-Mutant control, so that the whole genome can be quickly scanned for linkage.
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Igepal CA-630
Igepal CA-630
Igepal CA-630 is a non-ionic detergent widely used in biochemical research and applications.
It is a polyoxyethylene octylphenyl ether that serves as a mild, non-denaturing surfactant for solubilizing and stabilizing proteins and other biomolecules.
Researchers can leverge PubCompare.ai's AI-driven tools to optimize their Igepal CA-630 protocols, easily locate relevant literature, pre-prints and patents, and identify the best products and procedures for their needs.
This helps streamline Igepal CA-630 reserch and experimentation.
It is a polyoxyethylene octylphenyl ether that serves as a mild, non-denaturing surfactant for solubilizing and stabilizing proteins and other biomolecules.
Researchers can leverge PubCompare.ai's AI-driven tools to optimize their Igepal CA-630 protocols, easily locate relevant literature, pre-prints and patents, and identify the best products and procedures for their needs.
This helps streamline Igepal CA-630 reserch and experimentation.
Most cited protocols related to «Igepal CA-630»
Animals
Buffers
Centrifugation
Digestion
DNA Restriction Enzymes
Endopeptidase K
Endopeptidases
Enzymes
Gelatins
Genome
Helminths
Hermaphroditism
Heterozygote
Igepal CA-630
Magnesium Chloride
Males
Native Hawaiians
Oligonucleotide Primers
Sepharose
Tromethamine
Tween 20
X Chromosome
PCR templates were generated by cloning mutant animals from among the self-progeny of EG1000/CB4856 F1 hermaphrodites (described above) onto individual seeded plates. After 5 days, self progeny were washed from each plate using water (>100 worms / plate) and placed in a single well of a 96-well plate. Worms were allowed to settle to the bottom of the wells for 15' at 4°C then excess water was pipetted off to leave 45 μl in each well. The plates were frozen and stored at -80°C. The plates were thawed and 15 μl of 4X lysis buffer (200 mM KCl, 40 mM Tris pH 8.3, 10 mM MgCl2, 1.8% IGEPAL CA-630, 1.8% Tween 20, 0.04% (w/v) gelatin, 240 ug/ml proteinase K) was then added to each well to give 1X lysis buffer. The plates were covered with sealing tape and briefly vortexed to break up the worm pellet. The worms were lysed by incubation at 65°C 1 hour and 95°C 15 minutes. These PCR templates were stored frozen at -80°C and thawed prior to each use. For each PCR, each well of the 96-well plate received 9.8 μL of a PCR mix containing 8.5 μL water, 1 μL 10X buffer, 0.2 μL 10 mM dNTP, 0.02 μL each primer (100 μM), and 0.06 μL Taq (5 units/μl). Templates were then pin-replicated from the lysis plate. PCR conditions and DraI digests were the same as in chromosome mapping.
Animals
Buffers
Endopeptidase K
Freezing
Gelatins
Helminths
Hermaphroditism
Igepal CA-630
Magnesium Chloride
Oligonucleotide Primers
Tromethamine
Tween 20
Cell pellets were thawed on ice and incubated for 30 min at room temperature in 1 ml lysis buffer containing 150 mM NaCl, 50 mM Tris, pH 7.5, 5% glycerol, 1% IGEPAL-CA-630, 1 mM MgCl2, 200 U benzonase (Merck), and EDTA-free complete protease inhibitor cocktail (Roche). When studying phospho-dependent interactions, phosphatase inhibitors (Roche) were added as well. Lysates were cleared by centrifugation at 4,000 g and 4°C for 15 min to remove remaining membrane and DNA, and the supernatant was incubated with 50 µl magnetic beads coupled to monoclonal mouse anti-GFP antibody (Miltenyi Biotec) for 15 min on ice. Because of the extremely small size of the beads (50 nm), they are nonsedimenting and show fast reaction kinetics. Magnetic columns were equilibrated using 250 µl lysis buffer. Cell lysates were added to the column after incubation and washed three times with 800 µl ice-cold wash buffer I containing 150 mM NaCl, 50 mM Tris, pH 7.5, 5% glycerol, and 0.05% IGEPAL-CA-630, and two times with 500 µl of wash buffer II containing 150 mM NaCl, 50 mM Tris, pH 7.5, and 5% glycerol. Purified proteins were predigested by adding 25 µl 2 M urea in 50 mM Tris, pH 7.5, 1 mM DTT, and 150 ng EndoLysC (Wako Chemicals USA, Inc.) for SILAC experiments or 150 ng trypsin (Promega) for label-free experiments. After in-column digestion for 30 min at room temperature, proteins were eluted by adding two times 50 µl 2 M urea in 50 mM Tris, pH 7.5, and 5 mM chloroacetamide. In SILAC experiments, heavy and light eluates of transgenic cell line and the corresponding WT cell line were combined immediately after elution from the columns. Proteins were digested overnight at room temperature. The digestion was stopped by adding 1 µl trifluoroacetic acid, and peptides of each experiment were split and purified on two C18 Stage Tips and stored at 4°C (Rappsilber et al., 2007 (link)).
Pull-downs can be performed manually on a hand magnet. In our laboratory, pull-downs were performed on the automated liquid-handling platform (Freedom EVO 200; Tecan) in a fully automated manner.
Pull-downs can be performed manually on a hand magnet. In our laboratory, pull-downs were performed on the automated liquid-handling platform (Freedom EVO 200; Tecan) in a fully automated manner.
Animals, Transgenic
Antibodies, Anti-Idiotypic
Benzonase
Buffers
Cell Lines
Cells
Centrifugation
chloroacetamide
Cold Temperature
Digestion
Edetic Acid
Glycerin
Igepal CA-630
inhibitors
Kinetics
Lanugo
Magnesium Chloride
Mice, Laboratory
Pellets, Drug
Peptides
Phosphoric Monoester Hydrolases
Promega
Protease Inhibitors
Proteins
Sodium Chloride
Tissue, Membrane
TNFSF14 protein, human
Trifluoroacetic Acid
Tromethamine
Trypsin
Urea
Bath
BP 400
Buffers
Cell Extracts
Cell Lines
Cell Nucleus
Cells
Chromatin
Cytosol
Deoxycholate
Dietary Fiber
DNA Chips
Edetic Acid
Egtazic Acid
Formaldehyde
Glycerin
HEPES
Ice
Igepal CA-630
isolation
N-lauroylsarcosine
Nonidet P-40
Polypropylenes
Protease Inhibitors
Proteins
Sepharose
Sodium Chloride
Tissues
Triton X-100
Tromethamine
Trypan Blue
m6A MeRIP is based on the previously described m6A-seq protocol [27 (link)] with several modifications: 30 μl of protein A magnetic beads (10002D; Thermo Fisher Scientific) and 30 μl of protein G magnetic beads (10004D; Thermo Fisher Scientific) were washed twice by IP buffer (150 mM NaCl, 10 mM Tris-HCl [pH 7.5], 0.1% IGEPAL CA-630 in nuclease-free H2O), resuspended in 500 μl of IP buffer, and tumbled with 5 μg anti-m6A antibody at 4 °C for at least 6 hours. Following 2 washes in IP buffer, the antibody–bead mixture was resuspended in 500 μl of the IP reaction mixture containing fragmented total RNA, 100 μl of 5× IP buffer, and 5 μl of RNasin Plus RNase Inhibitor (N2611; Promega, Madison, WI) and incubated for 2 hours at 4 °C.
In the low/high salt-washing method, the RNA reaction mixture was washed twice in 1,000 μl of IP buffer, twice in 1,000 μl of low-salt IP buffer (50 mM NaCl, 10 mM Tris-HCl [pH 7.5], 0.1% IGEPAL CA-630 in nuclease-free H2O), and twice in 1,000 μl of high-salt IP buffer (500 mM NaCl, 10 mM Tris-HCl [pH 7.5], 0.1% IGEPAL CA-630 in nuclease-free H2O) for 10 minutes each at 4 °C. After extensive washing, the m6A-enriched fragmented RNA was eluted from the beads in 200 μl of RLT buffer supplied in RNeasy Mini Kit (74106; QIAGEN; Germany) for 2 minutes at room temperature. A magnetic separation rack was used to pull beads to the side of the tube. Supernatant was collected to a new tube, and 400 μl of 100% ethanol was added to it. The mixture was transferred to an RNeasy MiniElute spin column and centrifuged at >12,000 rpm at 4 °C for 1 minute. The spin column membrane was washed with 500 μl of RPE buffer once, then 500 μl of 80% ethanol once, and centrifuged at full speed for 5 minutes at 4 °C to remove the residual ethanol. The m6A-enriched RNA was eluted with 14 μl ultrapure H2O. For a second round of IP, eluted RNA was re-incubated with protein A/G magnetic beads coupled to anti-m6A antibody, followed by washes, elution from the protein A/G beads, and purification as above. In addition, it is the high salt that contributes to better S/N ratio (S7A Fig ).
In the m6A competitive elution method, the m6A competitive elution buffer for each pulldown was prepared by mixing 45 μl of 5× IP buffer, 75 μl of 20 mM m6A (M2780; Sigma-Aldrich), 3.5 μl of RNasin Plus RNase Inhibitor, and 101.5 μl of ultrapure H2O. The immunoprecipitated m6A RNA with protein A/G magnetic beads was then washed 3 times in 1,000 μl of IP buffer for 10 minutes each at 4 °C and was resuspended in 100 μl of m6A competitive elution buffer with continuous shaking for 1 hour at 4 °C. The mixture was placed on a magnetic separation rack, and supernatant containing the eluted m6A RNA was collected to a new tube. Then, another 100 μl of m6A competitive elution buffer was added for one more elution. To purify the eluted RNA, 700 μl of RLT buffer and 1,400 μl of 100% ethanol were added to 200 μl of eluted supernatant collected and mixed thoroughly. The mixture was transferred to an RNeasy MiniElute spin column (QIAGEN) and centrifuged at >12,000 rpm at 4 °C for 1 minute. This step was repeated until all of the sample was loaded to the column. The spin column membrane was washed with 500 μl of RPE buffer once, then 500 μl of 80% ethanol once, and centrifuged at full speed for 5 minutes at 4 °C to remove the residual ethanol. The m6A-enriched RNA was eluted with 14 μl ultrapure H2O.
The MeRIP-seq data has been deposited in NCBI GEO database (GSE116002).
In the low/high salt-washing method, the RNA reaction mixture was washed twice in 1,000 μl of IP buffer, twice in 1,000 μl of low-salt IP buffer (50 mM NaCl, 10 mM Tris-HCl [pH 7.5], 0.1% IGEPAL CA-630 in nuclease-free H2O), and twice in 1,000 μl of high-salt IP buffer (500 mM NaCl, 10 mM Tris-HCl [pH 7.5], 0.1% IGEPAL CA-630 in nuclease-free H2O) for 10 minutes each at 4 °C. After extensive washing, the m6A-enriched fragmented RNA was eluted from the beads in 200 μl of RLT buffer supplied in RNeasy Mini Kit (74106; QIAGEN; Germany) for 2 minutes at room temperature. A magnetic separation rack was used to pull beads to the side of the tube. Supernatant was collected to a new tube, and 400 μl of 100% ethanol was added to it. The mixture was transferred to an RNeasy MiniElute spin column and centrifuged at >12,000 rpm at 4 °C for 1 minute. The spin column membrane was washed with 500 μl of RPE buffer once, then 500 μl of 80% ethanol once, and centrifuged at full speed for 5 minutes at 4 °C to remove the residual ethanol. The m6A-enriched RNA was eluted with 14 μl ultrapure H2O. For a second round of IP, eluted RNA was re-incubated with protein A/G magnetic beads coupled to anti-m6A antibody, followed by washes, elution from the protein A/G beads, and purification as above. In addition, it is the high salt that contributes to better S/N ratio (
In the m6A competitive elution method, the m6A competitive elution buffer for each pulldown was prepared by mixing 45 μl of 5× IP buffer, 75 μl of 20 mM m6A (M2780; Sigma-Aldrich), 3.5 μl of RNasin Plus RNase Inhibitor, and 101.5 μl of ultrapure H2O. The immunoprecipitated m6A RNA with protein A/G magnetic beads was then washed 3 times in 1,000 μl of IP buffer for 10 minutes each at 4 °C and was resuspended in 100 μl of m6A competitive elution buffer with continuous shaking for 1 hour at 4 °C. The mixture was placed on a magnetic separation rack, and supernatant containing the eluted m6A RNA was collected to a new tube. Then, another 100 μl of m6A competitive elution buffer was added for one more elution. To purify the eluted RNA, 700 μl of RLT buffer and 1,400 μl of 100% ethanol were added to 200 μl of eluted supernatant collected and mixed thoroughly. The mixture was transferred to an RNeasy MiniElute spin column (QIAGEN) and centrifuged at >12,000 rpm at 4 °C for 1 minute. This step was repeated until all of the sample was loaded to the column. The spin column membrane was washed with 500 μl of RPE buffer once, then 500 μl of 80% ethanol once, and centrifuged at full speed for 5 minutes at 4 °C to remove the residual ethanol. The m6A-enriched RNA was eluted with 14 μl ultrapure H2O.
The MeRIP-seq data has been deposited in NCBI GEO database (GSE116002).
angiogenin
Antibodies, Anti-Idiotypic
Buffers
Cationic Protein, Eosinophil
Ethanol
G-substrate
GTP-Binding Proteins
Igepal CA-630
Immunoglobulins
Promega
Sodium Chloride
Staphylococcal Protein A
Tissue, Membrane
Tromethamine
Most recents protocols related to «Igepal CA-630»
Cells (5 × 104) were spun at 500 × g for 5 min, followed by a wash using 50 μL of cold 1× PBS and centrifugation at 500 × g for 5 min. Cells were lysed using cold lysis buffer (10 mM Tris-Cl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630). Immediately after lysis, nuclei were spun at 500 × g for 10 min using a refrigerated centrifuge. To avoid losing cells during the nuclei preparation, we used a fixed angle centrifuge and carefully pipetted away from the pellet after centrifugations. Immediately following the centrifugation, the pellet was resuspended in the transposase reaction mix (10 μL 5× TTBL buffer, 3 μL TTE Mix V50 (Illumina) and 37 μL of nuclease free water). The transposition reaction was carried out for 30 min at 37 °C. Directly following transposition the sample was purified using a DNA Clean&Concentrator-5 kit (ZYMO RESEARCH, D4014, California, USA). Following purification, we amplified library fragments using Q5® Hot Start High-Fidelity DNA Polymerase and PCR primers N5 and N7 (TruePrep® Index Kit V2 for Illumina, Vazyme, TD202), using the following PCR conditions: 72 °C for 5 min, 98 °C for 30 s, followed by thermocycling at 98 °C for 10 s, 63 °C for 30 s and 72 °C for 1 min. We amplified the full libraries for 13 cycles, after 13 cycles we took purification to the PCR reaction by VAHTS DNA Clean Beads (Vazyme, N411-01) according to the manufacturer’s instructions. Purified DNA was analyzed by high-throughput sequencing (Novogene, Beijing, China).
Buffers
Cell Nucleus
Cells
Centrifugation
Cold Temperature
DNA, A-Form
DNA-Directed DNA Polymerase
DNA Library
Igepal CA-630
Magnesium Chloride
Oligonucleotide Primers
Sodium Chloride
Transposase
Tromethamine
A20 and primary B lymphocytes were harvested from the culture and lysed for 10 min in RIPA buffer (150 mM NaCl, 1.0% IGEPAL CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0; Sigma) containing 1 X Halt protease/phosphatase inhibitors (Thermo Scientific). Following lysis, samples were clarified by centrifugation at 15,000 × g for 10 min at 4°C. Supernatants were collected and protein concentration was determined using the Pierce Rapid Gold BCA Protein Assay Kit (Thermo Scientific). Equal concentrations of protein extract were loaded into 4–12% NuPAGE BisTris gels (Life Technologies) and transferred to PVDF membranes utilizing the Transblot Turbo Transfer System (Bio-Rad). Membranes were blocked for 1 hr at room temperature in Odyssey Blocking Buffer in TBS (LI-COR) and incubated overnight at 4°C with primary antibody. The following antibodies and dilutions were used: MCU (1:2000; 14997 S, Cell Signaling Technology), GAPDH (1:5000; MAB374, Sigma), Total OXPHOS Rodent Antibody Cocktail (1:1000, ab110413, Abcam), NFAT1 (1:1000, 4389 S, Cell Signaling Technology), NFAT2 (1:1000, 8032 S, Cell Signaling Technology), α-Tubulin (1:5000, 3873 S, Cell Signaling Technology), phospho-CREB (Ser133, 1:1000, 9198 S, Cell Signaling Technology), and CREB (1:1000, 9104 S, Cell Signaling Technology). Membranes were washed with TBST and incubated for 1 hr at room temperature with the following secondary antibodies: IRDye 680RD goat anti-mouse (1:10,000 LI-COR) or IRDye 800RD donkey anti-rabbit (1:10,000 LI-COR). Membranes were imaged on an Odyssey CLx Imaging System (LI-COR) and analysis was performed in Image Studio Lite version 5.2 (LI-COR) and ImageJ.
alpha-Tubulin
Antibodies
B-Lymphocytes
Biological Assay
Bistris
Buffers
Centrifugation
Combined Antibody Therapeutics
Deoxycholic Acid, Monosodium Salt
Equus asinus
GAPDH protein, human
Gels
Goat
Gold
Igepal CA-630
Immunoglobulins
Mus
Phosphoric Monoester Hydrolases
polyvinylidene fluoride
Protease Inhibitors
Proteins
Rabbits
Radioimmunoprecipitation Assay
Rodent
Sodium Chloride
Technique, Dilution
Tissue, Membrane
Tromethamine
Cells were lysed in Radioimmunoprecipitation assay (RIPA) buffer (1% IGEPAL CA-630, 150 mM NaCl, 0.1% sodium dodecyl sulfate [SDS], 50 mM Tris pH 8.0, 1 mM Ethylenediaminetetraacetic acid (EDTA), 0.5% sodium deoxycholate) and protease inhibitor cocktail supplement (Roche, 11836170001). Protein concentration was determined by bicinchoninic acid assay (Pierce, 23227). 5× SDS loading buffer (235 mM SDS, 10% β-mercaptoethanol, 0.005% bromophenol blue, 210 mM Tris–HCl pH 6.8, 50% glycerol) was added to lysates to a final 1× concentration and incubated for 10 min at 90°C. Proteins were then resolved by SDS–polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane. Membranes were blocked using Odyssey Blocking Buffer (LI-COR Biosciences, P/N 927-40000). Antibodies used: anti-Cas9 (Diagenode, C15200229, 1:10,000) and anti-ERK (Cell Signaling Technologies, 4695S, 1:1000). Secondary antibodies used: anti-rabbit (LI-COR Biosciences, 926-32213, 1:10,000) and anti-mouse (LI-COR Biosciences, 926-32210, 1:10,000). Membranes were visualised using a LI-COR Odyssey CLx Infrared Imager.
2-Mercaptoethanol
Antibodies
bicinchoninic acid
Biological Assay
Bromphenol Blue
Buffers
Cardiac Arrest
Cells
Deoxycholic Acid, Monosodium Salt
Dietary Supplements
Edetic Acid
Glycerin
Igepal CA-630
Mus
Nitrocellulose
Protease Inhibitors
Proteins
Rabbits
Radioimmunoprecipitation Assay
SDS-PAGE
Sodium Chloride
Sulfate, Sodium Dodecyl
Tissue, Membrane
Tromethamine
Cholesterol levels were determined fluorometrically using the Amplex Red Cholesterol Assay kit (Thermo Fisher Sci.; A12216). Samples (0.5 × 105 cells) were extracted with chloroform:isopropanol:IGEPAL CA-630 (7:11:0.1) and centrifuged at 13,000 g for 10 min to remove insoluble material. The organic phase was dried under vacuum, dissolved in 1 × cholesterol reaction buffer, and analyzed following the guidelines provided by the supplier. To evaluate the intracellular distribution of the cholesterol load, cells were fixed in 4% paraformaldehyde (PFA) for 20 min at room temperature, and after washing three times, were incubated with the naturally fluorescent polyene antibiotic filipin III (0.25 mg/ml; Sigma-Aldrich, F4767) for 30 min. Images were acquired with a Zeiss Axiophot fluorescence microscope using a 40 × /1.3 N.A. objective. The ImageJ software [34 (link)] was used to calculate the corrected total cell fluorescence (CTCF) by applying the following formula: CTCF = Integrated Density − (Area of selected cell × Mean fluorescence of background readings). Mitochondria from SH-SY5Y cells were isolated by digitonin fractionation as described previously [35 ] and the GSH content was analyzed using the Glutathione Assay Kit (Sigma-Aldrich, CS0260-1 KT) following the manufacturer's instructions.
Antibiotics
Biological Assay
Buffers
Cells
Chloroform
Cholesterol
CTCF protein, human
Digitonin
Filipin
Fluorescence
Fractionation, Chemical
Glutathione
Igepal CA-630
Isopropyl Alcohol
Microscopy, Fluorescence
Mitochondria
paraform
Polyenes
Protoplasm
Vacuum
Cholesterol levels were determined fluorometrically using the Amplex Red Cholesterol Assay kit (Thermo Fisher Sci.; A12216). Samples (0.5 × 105 cells) were extracted with chloroform:isopropanol:IGEPAL CA-630 (7:11:0.1) and centrifuged at 13,000 g for 10 min to remove insoluble material. The organic phase was dried under vacuum, dissolved in 1 × cholesterol reaction buffer, and analyzed following the guidelines provided by the supplier. To evaluate the intracellular distribution of the cholesterol load, cells were fixed in 4% paraformaldehyde (PFA) for 20 min at room temperature, and after washing three times, were incubated with the naturally fluorescent polyene antibiotic filipin III (0.25 mg/ml; Sigma-Aldrich, F4767) for 30 min. Images were acquired with a Zeiss Axiophot fluorescence microscope using a 40 × /1.3 N.A. objective. The ImageJ software [34 (link)] was used to calculate the corrected total cell fluorescence (CTCF) by applying the following formula: CTCF = Integrated Density − (Area of selected cell × Mean fluorescence of background readings). Mitochondria from SH-SY5Y cells were isolated by digitonin fractionation as described previously [35 ] and the GSH content was analyzed using the Glutathione Assay Kit (Sigma-Aldrich, CS0260-1 KT) following the manufacturer's instructions.
Antibiotics
Biological Assay
Buffers
Cells
Chloroform
Cholesterol
CTCF protein, human
Digitonin
Filipin
Fluorescence
Fractionation, Chemical
Glutathione
Igepal CA-630
Isopropyl Alcohol
Microscopy, Fluorescence
Mitochondria
paraform
Polyenes
Protoplasm
Vacuum
Top products related to «Igepal CA-630»
Sourced in United States, Germany, United Kingdom, Macao, Italy, Switzerland, France
IGEPAL CA-630 is a non-ionic detergent used in various laboratory applications. It is a polyoxyethylene octylphenyl ether that acts as a wetting agent and emulsifier. The product assists in solubilizing and dispersing components in aqueous solutions.
Sourced in United States, Germany, China, United Kingdom, Italy, Japan, Sao Tome and Principe, France, Canada, Macao, Switzerland, Spain, Australia, Israel, Hungary, Ireland, Denmark, Brazil, Poland, India, Mexico, Senegal, Netherlands, Singapore
The Protease Inhibitor Cocktail is a laboratory product designed to inhibit the activity of proteases, which are enzymes that can degrade proteins. It is a combination of various chemical compounds that work to prevent the breakdown of proteins in biological samples, allowing for more accurate analysis and preservation of protein integrity.
Sourced in United States, Switzerland, Germany, China, United Kingdom, France, Canada, Japan, Italy, Australia, Austria, Sweden, Spain, Cameroon, India, Macao, Belgium, Israel
Protease inhibitor cocktail is a laboratory reagent used to inhibit the activity of proteases, which are enzymes that break down proteins. It is commonly used in protein extraction and purification procedures to prevent protein degradation.
Sourced in Germany, United States
The MinElute kit is a laboratory equipment used for DNA and RNA purification. It facilitates the efficient extraction and concentration of nucleic acids from various sample types.
Sourced in Germany, United States, United Kingdom, Italy, Japan, Netherlands, China, France, Switzerland
The MinElute PCR Purification Kit is a laboratory equipment product designed for the efficient purification of PCR amplicons. It utilizes a silica-membrane-based technology to capture and purify DNA fragments from PCR reactions, allowing for the removal of primers, nucleotides, enzymes, and other impurities.
Sourced in United States, Germany, Switzerland, United Kingdom, China, France, Japan, Canada, Spain, Belgium, Australia, Sweden, Italy, Ireland, Macao
The Complete Protease Inhibitor Cocktail is a laboratory product designed to inhibit a broad spectrum of proteases. It is a concentrated solution containing a mixture of protease inhibitors effective against a variety of protease classes. This product is intended to be used in research applications to preserve the integrity of target proteins by preventing their degradation by proteolytic enzymes.
Sourced in United States, Germany, China, United Kingdom, Morocco, Ireland, France, Italy, Japan, Canada, Spain, Switzerland, New Zealand, India, Hong Kong, Sao Tome and Principe, Sweden, Netherlands, Australia, Belgium, Austria
PVDF membranes are a type of laboratory equipment used for a variety of applications. They are made from polyvinylidene fluoride (PVDF), a durable and chemically resistant material. PVDF membranes are known for their high mechanical strength, thermal stability, and resistance to a wide range of chemicals. They are commonly used in various filtration, separation, and analysis processes in scientific and research settings.
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AMPure XP beads are a magnetic bead-based product used for the purification of nucleic acids, such as DNA and RNA, from various samples. The beads are designed to selectively bind to nucleic acids, allowing for the removal of contaminants and unwanted molecules during the purification process. The core function of AMPure XP beads is to provide an efficient and reliable method for the cleanup and concentration of nucleic acids in preparation for downstream applications.
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The HiSeq 2500 is a high-throughput DNA sequencing system designed for a wide range of applications, including whole-genome sequencing, targeted sequencing, and transcriptome analysis. The system utilizes Illumina's proprietary sequencing-by-synthesis technology to generate high-quality sequencing data with speed and accuracy.
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
More about "Igepal CA-630"
Igepal CA-630 is a non-ionic detergent that is widely utilized in biochemical research and applications.
Also known as octylphenol ethoxylate, it serves as a mild, non-denaturing surfactant that helps solubilize and stabilize proteins and other biomolecules.
Researchers can leverage the AI-driven tools provided by PubCompare.ai to optimize their Igepal CA-630 protocols, easily locate relevant literature, preprints, and patents, and identify the best products and procedures for their needs.
This helps streamline Igepal CA-630 research and experimentation.
Igepal CA-630 is often used in conjunction with other research tools and techniques, such as protease inhibitor cocktails, MinElute kits, PVDF membranes, AMPure XP beads, and HiSeq 2500 sequencing platforms.
The detergent's ability to solubilize and stabilize biomolecules makes it useful for a variety of applications, including protein purification, western blotting, and nucleic acid extraction and purification.
Researchers can leverage the power of PubCompare.ai's AI-driven tools to compare and optimize their Igepal CA-630 protocols, ensuring they are using the most effective and efficient methods for their research needs.
By easily locating relevant literature, preprints, and patents, researchers can stay up-to-date on the latest advancements in Igepal CA-630 usage and best practices.
This helps streamline the research process and ensures researchers are using the most effective protocols and products for their Igepal CA-630-based experiments.
Also known as octylphenol ethoxylate, it serves as a mild, non-denaturing surfactant that helps solubilize and stabilize proteins and other biomolecules.
Researchers can leverage the AI-driven tools provided by PubCompare.ai to optimize their Igepal CA-630 protocols, easily locate relevant literature, preprints, and patents, and identify the best products and procedures for their needs.
This helps streamline Igepal CA-630 research and experimentation.
Igepal CA-630 is often used in conjunction with other research tools and techniques, such as protease inhibitor cocktails, MinElute kits, PVDF membranes, AMPure XP beads, and HiSeq 2500 sequencing platforms.
The detergent's ability to solubilize and stabilize biomolecules makes it useful for a variety of applications, including protein purification, western blotting, and nucleic acid extraction and purification.
Researchers can leverage the power of PubCompare.ai's AI-driven tools to compare and optimize their Igepal CA-630 protocols, ensuring they are using the most effective and efficient methods for their research needs.
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