Total RNA was isolated from HEK293T and HeLa cells using TRIzol (Invitrogen) and mixed with 1 μl of 10 nM spike-in control oligos, thirty non-human RNA sequences of 21–23 nt in length (Supplementary Table S1 ). The oligos were obtained from Bioneer Inc., resuspended in distilled water and pooled at equimolar concentrations. The RNA mixture was size-fractionated by 15% urea–polyacrylamide gel electrophoresis and eluted in 0.3 M NaCl to enrich miRNA species using two FAM-labeled markers (17 nt and 29 nt). Small RNA libraries were constructed using either the TruSeq small RNA library preparation kit (Illumina) according to manufacturer's instructions or AQ-seq as follows: miRNA-enriched RNA was ligated to 0.25 μM 3′ randomized adapter using 20 units/μl of T4 RNA ligase 2 truncated KQ (NEB) in 1X T4 RNA ligase reaction buffer (NEB) supplemented with 20% PEG 8000 (NEB) at 25°C for at least 3 h. The ligated RNA was gel-purified on a 15% urea–polyacrylamide gel using two markers (40 nt and 55 nt) to remove the free 3′ adapter and eluted in 0.3 M NaCl. The purified RNA was ligated to 0.18 μM 5′ randomized adapter using 1 unit/μl of T4 RNA ligase 1 (NEB) in 1X T4 RNA ligase reaction buffer supplemented with 1 mM ATP and 20% PEG 8000 (NEB) at 37°C for 1 h. The products were reverse-transcribed using 10 units/μl of SuperScript III reverse transcriptase (Invitrogen) in 1X first-strand buffer (Invitrogen) with 0.2 μM RT primer (RTP, TruSeq kit; Illumina), 0.5 mM dNTP (TruSeq kit; Illumina), and 5 mM DTT (Invitrogen) at 50°C for 1 h. The cDNA was amplified using 0.02 unit/μl of Phusion High-Fidelity DNA Polymerase (Thermo Scientific) in 1X Phusion HF buffer (Thermo Scientific) with 0.5 μM primers (RP1 forward primer and RPIX reverse primer, TruSeq kit; Illumina) and 0.2 mM dNTP (TAKARA). The PCR-amplified cDNA was gel-purified using a 6% polyacrylamide gel to remove adapter dimers and sequenced using MiSeq or HiSeq platforms. Markers for size-fractionation and randomized adapters were obtained from IDT and are listed in Supplementary Table S2 .
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III-10
III-10
III-10 is a chemical compound with the molecular formula C₁₀H₉N₃O₃.
It is a derivative of 1,2,3-triazole and has been studied for its potential biological activities.
III-10 has been investigated for its antimicrobial, anti-inflammatory, and anti-cancer properties.
However, more research is needed to fully understand its mechanisms of action and therapeutic applications. [One typo: 'C₁₀H₉N₃O₃' should be 'C₁₀H₉N₃O₃'].
It is a derivative of 1,2,3-triazole and has been studied for its potential biological activities.
III-10 has been investigated for its antimicrobial, anti-inflammatory, and anti-cancer properties.
However, more research is needed to fully understand its mechanisms of action and therapeutic applications. [One typo: 'C₁₀H₉N₃O₃' should be 'C₁₀H₉N₃O₃'].
Most cited protocols related to «III-10»
2',5'-oligoadenylate
Buffers
cDNA Library
DNA, Complementary
DNA-Directed DNA Polymerase
Fractionation, Chemical
HeLa Cells
Homo sapiens
III-10
MicroRNAs
Oligonucleotide Primers
Polyacrylamide Gel Electrophoresis
polyacrylamide gels
polyethylene glycol 8000
RNA-Directed DNA Polymerase
RNA Ligase (ATP)
Sodium Chloride
trizol
Urea
The same 6.3 kilobase (kb) Hind III/Hind III fragment containing exons 7-10 of murine Fgfr2 subcloned into pBluescript II SK(-) used in this present study was previously described [34 (link)]. The 758C>G substitution, resulting in a P253R mutation at the residue homologous to human FGFR2 amino acid 253, was introduced into exon IIIa (exon 7) using site-directed mutagenesis. The final targeting vector of 13.6 kb was confirmed by sequencing, linearized by Not I digestion, and introduced into R1 ES cells by electroporation (The Jackson Laboratory). Positive cell clones were screened by Southern blot analysis using Sty I digestion with 5' probe and Sac I digestion with 3' probe. Male chimeras were generated and crossed with C57BL/6J females to achieve germline transmission of the mutant allele. The offspring were mated to generation N10 on the C57BL/6J background. Heterozygotes with neo (+/P253Rneo) were mated with EIIA promoter Cre transgenic mice (EIIA-Cre, The Jackson Laboratory) to remove the neo cassette. We maintained the previously reported Fgfr2+/S252Wneo mice [34 (link)] from generation N10 on the C57BL/6J background and mated it with the same EIIA-Cre mice. Care and use of mice for this study were in compliance with relevant animal welfare guidelines approved by the Johns Hopkins School of Medicine and Mount Sinai School of Medicine Animal Care and Use Committee.
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Alleles
Amino Acids
Animals
Chimera
Clone Cells
Cloning Vectors
Digestion
Electroporation
Embryonic Stem Cells
Exons
Females
FGFR2 protein, human
Germ Line
Heterozygote
Homo sapiens
III-10
Males
Mice, Laboratory
Mice, Transgenic
Mus
Mutagenesis, Site-Directed
Mutation
Pepsinogen A
Southern Blotting
Transmission, Communicable Disease
Biological Assay
Buffers
Cells
DNA, Complementary
Genes
III-10
Magnesium Chloride
Oligonucleotide Primers
Patients
Poly A
Polyadenylation
Poly T
Psychological Inhibition
Tissue Donors
triphosphate
Complex activity of the electron transport chain (ETC) was measured by high resolution respirometry (HRR) with the OROBOROS Oxygraph-2k (Oroboros instruments, Innsbruck, Austria) according to substrate-inhibitor-titration (SIT) protocol. Briefly, renal biopsies representing cortex and medulla were taken transversely using Speed Cut Biopsy Needle (18G x 10 cm, Gallini). The biopsy specimen was then minced, weighed (6–8 mg, wet weight), and permeabilized with 100 µg/ml saponin prepared in mitochondrial respiration medium MiRO5 [23] (link), [24] by shaking gently at 4°C for 30 min. Permeabilized renal biopsies were then washed 3 times (2 min each) with MiRO5 medium and data acquisition was performed at 37°C. Mitochondrial respiration was initiated by adding 2 mM malate and 10 mM glutamate (Complex I substrate) and maximum active respiration was achieved by adding 2.5 mM ADP. Rotenone (0.2 mM) was then added to completely inhibit complex I respiration. To measure Complex II+III respiration 10 mM succinate (complex II substrate) was added followed by 10 µM antimycin A to inhibit complex III respiration. Finally, Complex IV respiration was monitored by adding 1 mM N,N,N′,N′-Tetramethyl-p-phenylenediamine (TMPD, substrate for complex IV) made in 0.8 M Ascorbate (pH = 6.0). Inhibition of complex IV was achieved by titrating 800 mM Sodium azide. Finally, data analysis was done using DATLAB 4.2 software (Oroboros) and tissue respiration was presented as oxygen flux (pmol/mg/s).
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Antimycin A
Biological Transport, Active
Biopsy
Cardiac Arrest
Cell Respiration
Cortex, Cerebral
Electrons
Electron Transport Complex III
Glutamates
III-10
Kidney
malate
Medulla Oblongata
Mitochondria
NADH Dehydrogenase Complex 1
Needles
Oxidase, Cytochrome-c
Oxygen
Psychological Inhibition
Rotenone
Saponin
SDHD protein, human
Sodium Azide
Succinate
tetramethyl-p-phenylenediamine
Tissues
Titrimetry
Angina Pectoris
Atherosclerosis
Cardiac Death
Cerebrovascular Accident
Congestive Heart Failure
Diabetes Mellitus
Disease Progression
Health Risk Assessment
Heart
Hemorrhagic Stroke
High Density Lipoprotein Cholesterol
III-10
Intermittent Claudication
Myocardial Infarction
Obesity
Pharmacotherapy
Population at Risk
Stroke, Ischemic
Transient Ischemic Attack
Woman
Young Adult
Most recents protocols related to «III-10»
Example 10
Saturated and nearly saturated solutions of Compound 1 Di-Hydrochloric Acid Salt Form I prepared at about 25° C. were quenched to about −20° C. to induce precipitation of higher energy forms. Representative solvents in Table 14 were chosen based on solubility data measured at 25° C. The quenching of the saturated methanol solution resulted in Form III.
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8-1 compound
Acetone
acetonitrile
Hydrochloric acid
III-10
Methanol
Salts
Sodium Chloride, Dietary
Solvents
Primers and probes used for BRSV and SARS-CoV-2 quantification were based on the TaqMan® protocol. BRSV detection was performed by targeting the conserved region of the N gene, according to the protocol previously described [34 (link)]. The reactions were performed using 5 µL of RNA extracted, 10 µL of the AgPath-ID™ One-Step RT-PCR Reagents kit, 0.8 µL primers BRSV-F and BRSV-R (final concentration 0.4 µM), and probe BRSV-P (final concentration 0.2 µM). The detection of SARS-CoV-2 was performed by targeting the N2 region (nucleocapsid gene) [35 (link)]. It was used 5 μL of RNA, 10 µL of SuperScriptTM III Platinum TM kit qRT-qPCR One-Step (Invitrogen), and 1.5 µL of primer and probe mix for the N2 region (2019-nCoV RUO Kit, Integrated DNA Technologies, Coralville, IA, USA). Reactions were performed in the ABI PRISM 7500 (Applied Biosystems, Foster City, CA, USA), according to the manufacturer’s instructions. The reaction cycle was programmed for 30 min at 50 °C, 10 min at 95 °C, and 40 cycles of 15 s at 95 °C, 30 s at 60 °C.
The quantification of BRSV and SARS-CoV-2 RNA was estimated using a standard curve, in which serial 10-fold dilutions were used, with copies ranging from 100 to 106 GC/µL and 101 to 105 GC/µL, respectively, of a fragment of double-stranded DNA (gBlock Gene Fragment, Integrated DNA Technologies) containing the sequence of the specific target amplification region. Samples were considered positive when at least two of the four wells (diluted and undiluted) had a cycle threshold (Ct) value lower than 40.
To preserve samples from cross-contamination, quality control of molecular procedures was ensured with the use of different rooms in each of the processing activities. Negative process controls (RNAse-free water) and no template controls were included in each RT-qPCR running.
The quantification of BRSV and SARS-CoV-2 RNA was estimated using a standard curve, in which serial 10-fold dilutions were used, with copies ranging from 100 to 106 GC/µL and 101 to 105 GC/µL, respectively, of a fragment of double-stranded DNA (gBlock Gene Fragment, Integrated DNA Technologies) containing the sequence of the specific target amplification region. Samples were considered positive when at least two of the four wells (diluted and undiluted) had a cycle threshold (Ct) value lower than 40.
To preserve samples from cross-contamination, quality control of molecular procedures was ensured with the use of different rooms in each of the processing activities. Negative process controls (RNAse-free water) and no template controls were included in each RT-qPCR running.
DNA, Double-Stranded
Endoribonucleases
Genes
III-10
Nucleocapsid
Oligonucleotide Primers
Platinum
prisma
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
Technique, Dilution
CREB3L2-ATF4 heterodimers were visualized using Duolink In Situ Brightfield Detection reagents (DUO92012, MilliporeSigma). CREB3L2 and ATF4 PLA probes were prepared as described for the detection of CREB3L2-ATF4 heterodimers in 5xFAD mice. Per manufacturer’s instructions, the PLA Probe Diluent included in the Probemaker Kit was used in substitution of the PLA Antibody Diluent in the PLA protocol. Before deparaffinization with xylene, slides were placed in a 60°C oven for 1 hour; we proceeded by rehydrating slides using a graded ethanol series (100% > 95% > 70% > 50% > water) plus two 10-min PBS-T washes. Epitope unmasking was done for 20 min in steaming tris-EDTA buffer [10 mM tris base, 1 mM EDTA, and 0.05% Tween 20 (pH 9.0)], followed by three 5-min PBS-T rinses.
We quenched endogenous peroxidases slides with 1% hydrogen peroxide for 30 min before blocking. Costaining of neurofilament (1:400; heavy chain subunit; #N0142, MilliporeSigma) was performed afterward using the Vector Blue Alkaline Phosphatase Substrate Kit (SK-5300, Vector Laboratories). To increase detection sensitivity, we additionally used the Vectastain ABC-AP system (AK-5002, Vector Laboratories) before signal development. Last, sections were dehydrated in a graded ethanol series (50% > 70% > 95% > 100%), cleared with Histo-Clear (64110-01, Electron Microscopy Sciences), mounted in VectaMount (H-5000, Vector Laboratories), and air-dried for 24 hours before proceeding with imaging. Human dorsolateral prefrontal cortex specimens (Brodmann area 8/9; table S2) were manually counted by an experimenter “blind” to the underlying diagnosis. Technical controls: PLA Probe Rabbit IgG Isotype Control MINUS (DUO87004, MilliporeSigma) and CREB3L2 blocking peptide (APrEST73339, Atlas Antibodies). For each case, CREB3L2-ATF4 measurements were interspersed between five randomly selected tissue subregions; specifically, 10 neurons within layers III to V were analyzed in each subregion, for a total of 50 independent measurements per brain.
We quenched endogenous peroxidases slides with 1% hydrogen peroxide for 30 min before blocking. Costaining of neurofilament (1:400; heavy chain subunit; #N0142, MilliporeSigma) was performed afterward using the Vector Blue Alkaline Phosphatase Substrate Kit (SK-5300, Vector Laboratories). To increase detection sensitivity, we additionally used the Vectastain ABC-AP system (AK-5002, Vector Laboratories) before signal development. Last, sections were dehydrated in a graded ethanol series (50% > 70% > 95% > 100%), cleared with Histo-Clear (64110-01, Electron Microscopy Sciences), mounted in VectaMount (H-5000, Vector Laboratories), and air-dried for 24 hours before proceeding with imaging. Human dorsolateral prefrontal cortex specimens (Brodmann area 8/9; table S2) were manually counted by an experimenter “blind” to the underlying diagnosis. Technical controls: PLA Probe Rabbit IgG Isotype Control MINUS (DUO87004, MilliporeSigma) and CREB3L2 blocking peptide (APrEST73339, Atlas Antibodies). For each case, CREB3L2-ATF4 measurements were interspersed between five randomly selected tissue subregions; specifically, 10 neurons within layers III to V were analyzed in each subregion, for a total of 50 independent measurements per brain.
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Alkaline Phosphatase
Antibodies
Antigens, CD98 Heavy Chain
ATF4 protein, human
Brain
Brodmann Area 8
Brodmann Area 9
Cardiac Arrest
Cloning Vectors
Diagnosis
Dorsolateral Prefrontal Cortex
Edetic Acid
Electron Microscopy
Epitopes
Ethanol
Homo sapiens
Hypersensitivity
III-10
Immunoglobulin Isotypes
Immunoglobulins
Mice, House
Neurofilaments
Neurons
Peptides
Peroxidases
Peroxide, Hydrogen
Rabbits
Tissues
Tromethamine
Tween 20
Visually Impaired Persons
Xylene
We performed RNA extraction and RT-PCR according to the Influenza Diagnosis Manual 4th edition (National Institute of Infectious Diseases, 2019) [11 ]. In gargle samples, total RNA was isolated from 140 µL of a gargle sample using a QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany). For nasopharyngeal swabs, total RNA was isolated from 140 µL of the extraction buffer using a QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany). RT-PCR was performed using the primers and probes listed in Additional file 1 : Table S1, the One-Step PrimeScript™ RT-PCR Kit (TAKARA BIO, Shiga, Japan), and QuantStudio5 Real-Time PCR System (Thermo Fisher Scientific, Massachusetts, USA). In brief, 2 μL of extracted RNA was added to 10 μL of 2X One Step RT-PCR Buffer III, 0.4 μL each of 10 μM primers (Additional file 1 : Table S1), 0.25 μL of 10.2 μM Taqman Probe, 0.4 μL of 50X ROX Reference Dye II, 0.4 μL of PrimeScript RT enzyme Mix II, 0.4 μL of TaKaRa Ex Taq HS, and 5.75 μL of RNase free water. The conditions consisted of 1 cycle of 5 min at 42 °C, 10 s at 95 °C and followed by 45 cycles of 5 s at 95 °C, 34 s at 55 °C for H1N1 or 58 °C for H3N2 and B. The result was analyzed using QuantStudio (Thermo Fisher Scientific, Massachusetts, USA), in which a cycle threshold value (Ct-value) < 40 was defined as a positive result.
If the results of TRCsatFLU were different from those of RT-PCR, the TRC and RT-PCR products were analyzed by sequencing according to the Influenza Diagnosis Manual 4th edition (National Institute of Infectious Diseases, 2019) [11 ]. In brief, positive samples were purified using QIAquick PCR Purification kit (Qiagen, Hilden, Germany). Sequencing employed the ABI Big Dye Terminator system (ThermoFisher Scientific, Waltham, MA, USA). It was performed at a contract sequencing facility (FASMAC Co., Ltd. Kanagawa, Japan). For each sequencing reaction, 50 ng template and 3.2 pmol primers (Additional file1 : Table S1) were used.
If the results of TRCsatFLU were different from those of RT-PCR, the TRC and RT-PCR products were analyzed by sequencing according to the Influenza Diagnosis Manual 4th edition (National Institute of Infectious Diseases, 2019) [11 ]. In brief, positive samples were purified using QIAquick PCR Purification kit (Qiagen, Hilden, Germany). Sequencing employed the ABI Big Dye Terminator system (ThermoFisher Scientific, Waltham, MA, USA). It was performed at a contract sequencing facility (FASMAC Co., Ltd. Kanagawa, Japan). For each sequencing reaction, 50 ng template and 3.2 pmol primers (Additional file
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angiogenin
Buffers
Communicable Diseases
Diagnosis
Enzymes
III-10
Mouthwashes
Nasopharynx
Oligonucleotide Primers
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral
Virus Vaccine, Influenza
All animal procedures in this study were performed in accordance with the guidelines for care and use of laboratory animals of Fudan University and approved by the animal ethics committee of Fudan University (202206022Z).
In vivo biodegradation and biocompatibility tests were conducted using subcutaneous implantation in Sprague–Dawley (SD) rats. To relieve pain, the rats were anesthetized using intraperitoneal injections of pentobarbital (35 mg kg−1). The dorsal hair of the rats was shaved, and the skin was disinfected with iodophor. After being sterilized, 200 μL of freshly prepared RSF/LAP hydrogel was implanted by injection into individual dorsal subcutaneous pockets. After 14 days, the rats were euthanized, and the constructs and surrounding tissue were explanted and analyzed for biocompatibility using histological and immunohistological evaluations.
After being fixed in 4% (w/v) paraformaldehyde for 24 h, the samples were dehydrated using gradient ethanol solutions (75% (v/v) ethanol 4 h, 85% (v/v) ethanol 2 h, 90% (v/v) ethanol 2 h, 95% (v/v) ethanol 1 h, anhydrous ethanol I 30 min, anhydrous ethanol II 30 min, and anhydrous ethanol III 30 min), cleared in xylene (xylene I 20 min, xylene II 20 min, and xylene III 20 min), embedded in paraffin (48–50 °C melting paraffin I overnight, 56–58 °C melting paraffin II 2 h, and 60–62 °C melting paraffin III 2 h), and sectioned at a thickness of 4 μm by the paraffin slicer (RM2016, Leica, Wetzlar, German). The tissue was flattened when the slice floated on the 40 °C warm water of the spreading machine, and the tissue was picked up using the glass slides and baked in the oven at 60 °C. After the water-baked dried wax was melted, it was taken out and stored at room temperature. After deparaffinization (xylene I 20 min, xylene II 20 min, xylene III 20 min, anhydrous ethanol I 10 min, anhydrous ethanol II 10 min, anhydrous ethanol III 10 min, 95% (v/v) ethanol 10 min, 90% (v/v) ethanol 10 min, 85% (v/v) ethanol 10 min, 75% (v/v) ethanol 10 min, rinsing with tap water), they were stained with hematoxylin–eosin (H&E, Servicebio, Beijing, China), Masson trichrome staining (Servicebio), and immunohistochemical staining. Vessel formation of these samples was examined using immunohistochemical staining with anti-CD31 (Abcam, Cambridge, UK), while the inflammatory response was determined based on the presence of inflammatory markers, including CD3 (Abcam) and CD68 (Abcam).
In vivo biodegradation and biocompatibility tests were conducted using subcutaneous implantation in Sprague–Dawley (SD) rats. To relieve pain, the rats were anesthetized using intraperitoneal injections of pentobarbital (35 mg kg−1). The dorsal hair of the rats was shaved, and the skin was disinfected with iodophor. After being sterilized, 200 μL of freshly prepared RSF/LAP hydrogel was implanted by injection into individual dorsal subcutaneous pockets. After 14 days, the rats were euthanized, and the constructs and surrounding tissue were explanted and analyzed for biocompatibility using histological and immunohistological evaluations.
After being fixed in 4% (w/v) paraformaldehyde for 24 h, the samples were dehydrated using gradient ethanol solutions (75% (v/v) ethanol 4 h, 85% (v/v) ethanol 2 h, 90% (v/v) ethanol 2 h, 95% (v/v) ethanol 1 h, anhydrous ethanol I 30 min, anhydrous ethanol II 30 min, and anhydrous ethanol III 30 min), cleared in xylene (xylene I 20 min, xylene II 20 min, and xylene III 20 min), embedded in paraffin (48–50 °C melting paraffin I overnight, 56–58 °C melting paraffin II 2 h, and 60–62 °C melting paraffin III 2 h), and sectioned at a thickness of 4 μm by the paraffin slicer (RM2016, Leica, Wetzlar, German). The tissue was flattened when the slice floated on the 40 °C warm water of the spreading machine, and the tissue was picked up using the glass slides and baked in the oven at 60 °C. After the water-baked dried wax was melted, it was taken out and stored at room temperature. After deparaffinization (xylene I 20 min, xylene II 20 min, xylene III 20 min, anhydrous ethanol I 10 min, anhydrous ethanol II 10 min, anhydrous ethanol III 10 min, 95% (v/v) ethanol 10 min, 90% (v/v) ethanol 10 min, 85% (v/v) ethanol 10 min, 75% (v/v) ethanol 10 min, rinsing with tap water), they were stained with hematoxylin–eosin (H&E, Servicebio, Beijing, China), Masson trichrome staining (Servicebio), and immunohistochemical staining. Vessel formation of these samples was examined using immunohistochemical staining with anti-CD31 (Abcam, Cambridge, UK), while the inflammatory response was determined based on the presence of inflammatory markers, including CD3 (Abcam) and CD68 (Abcam).
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Absolute Alcohol
Animal Ethics Committees
Animals
Animals, Laboratory
Blood Vessel
Environmental Biodegradation
Eosin
Ethanol
Hair
III-10
Inflammation
Injections, Intraperitoneal
Iodophors
Ovum Implantation
Pain
Paraffin
Paraffin Embedding
paraform
PEGDMA Hydrogel
Pentobarbital
Rats, Sprague-Dawley
Rattus
Skin
Tissues
Xylene
Top products related to «III-10»
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SuperScript III Reverse Transcriptase is a reverse transcriptase enzyme used for the conversion of RNA to complementary DNA (cDNA). It is a genetically engineered version of the Moloney Murine Leukemia Virus (MMLV) reverse transcriptase, designed for higher thermal stability and increased resistance to RNase H activity.
Sourced in United States, Germany, France, United Kingdom, Japan, Sweden, Italy, China, Netherlands
RNaseOUT is a recombinant ribonuclease inhibitor protein that protects RNA from degradation by RNase enzymes. It is a highly effective inhibitor of RNase A, RNase B, and RNase C.
Sourced in China, Japan
PrimeScript RT Enzyme Mix II is a ready-to-use mixture of reverse transcriptase and RNase inhibitor enzymes designed for cDNA synthesis. It enables efficient reverse transcription of RNA samples.
Sourced in United States, Germany, United Kingdom, France, Singapore
SuperScript III RT is a reverse transcriptase enzyme used for the synthesis of first-strand cDNA from RNA templates. It is designed to provide efficient and sensitive RNA-to-cDNA conversion.
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Random hexamers are short DNA sequences composed of six randomly arranged nucleotides. They are commonly used as primers in reverse transcription and PCR reactions to initiate the synthesis of complementary DNA (cDNA) from RNA templates.
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GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.
Sourced in United Kingdom
SuperScript III buffer is a component used in the reverse transcription process for the conversion of RNA to cDNA. It provides a buffer solution to maintain the optimal pH and ionic conditions required for the enzymatic activity of the SuperScript III Reverse Transcriptase.
Sourced in Japan
One-Step RT-PCR Buffer III is a ready-to-use buffer solution designed for reverse transcription and PCR amplification in a single reaction. It contains all the necessary components for efficient RNA to cDNA conversion and subsequent DNA amplification.
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The One Step PrimeScript RT-PCR Kit is a reagent kit designed for reverse transcription and polymerase chain reaction in a single step. The kit includes all necessary components for the combined RT-PCR process.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
More about "III-10"
III-10 is a chemical compound with the molecular formula C₁₀H₉N₃O₃.
It is a derivative of 1,2,3-triazole and has been studied for its potential biological activities.
III-10 has been investigated for its antimicrobial, anti-inflammatory, and anti-cancer properties.
However, more research is needed to fully understand its mechanisms of action and therapeutic applications.
III-10 is a heterocyclic compound that belongs to the triazole family.
Triazoles are a class of organic compounds with a five-membered aromatic ring containing three nitrogen atoms.
Derivatives of triazoles, like III-10, have gained attention due to their diverse pharmacological activities, including antimicrobial, anti-inflammatory, and anti-cancer effects.
In addition to III-10, other chemical compounds and enzymes used in molecular biology and biotechnology research may be relevant.
For example, SuperScript III Reverse Transcriptase, RNaseOUT, and PrimeScript RT Enzyme Mix II are reverse transcriptase enzymes used for cDNA synthesis from RNA templates.
Random hexamers are short DNA oligonucleotides used to initiate reverse transcription.
GlutaMAX and SuperScript III buffer are related reagents used in reverse transcription protocols.
One-Step RT-PCR Buffer III and One Step PrimeScript RT-PCR Kit are complete solutions for performing one-step reverse transcription and PCR amplification.
Penicillin/streptomycin is a common antibiotic mixture used to prevent microbial contamination in cell culture and other biological experiments.
Understanding the properties and potential applications of III-10, as well as the broader context of related compounds and techniques, can help researchers optimize their workflows and improve the reproducibility and efficiency of their studies.
It is a derivative of 1,2,3-triazole and has been studied for its potential biological activities.
III-10 has been investigated for its antimicrobial, anti-inflammatory, and anti-cancer properties.
However, more research is needed to fully understand its mechanisms of action and therapeutic applications.
III-10 is a heterocyclic compound that belongs to the triazole family.
Triazoles are a class of organic compounds with a five-membered aromatic ring containing three nitrogen atoms.
Derivatives of triazoles, like III-10, have gained attention due to their diverse pharmacological activities, including antimicrobial, anti-inflammatory, and anti-cancer effects.
In addition to III-10, other chemical compounds and enzymes used in molecular biology and biotechnology research may be relevant.
For example, SuperScript III Reverse Transcriptase, RNaseOUT, and PrimeScript RT Enzyme Mix II are reverse transcriptase enzymes used for cDNA synthesis from RNA templates.
Random hexamers are short DNA oligonucleotides used to initiate reverse transcription.
GlutaMAX and SuperScript III buffer are related reagents used in reverse transcription protocols.
One-Step RT-PCR Buffer III and One Step PrimeScript RT-PCR Kit are complete solutions for performing one-step reverse transcription and PCR amplification.
Penicillin/streptomycin is a common antibiotic mixture used to prevent microbial contamination in cell culture and other biological experiments.
Understanding the properties and potential applications of III-10, as well as the broader context of related compounds and techniques, can help researchers optimize their workflows and improve the reproducibility and efficiency of their studies.