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Immobilon P

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Most cited protocols related to «Immobilon P»

1
SDS-PAGE and Western Blot Analysis
Cited 44 times
Protein samples were treated for 30 min at 50°C in a sample-treating solution containing 2% SDS and 5% β-mercaptoethanol. The samples were then loaded onto a 12% or 15% SDS-polyacrylamide gel (7.0×8.3 cm ×0.75 mm) and electrophoresed using Mini-Protean Tetra system (Bio-Rad) at 15 mA (current constant). After electrophoresis, proteins separated on the gel were transferred onto a methanol-activated PVDF membrane (Immobilon-P, pore size 0.45 µm, Millipore) or a nitrocellulose membrane (Protran BA85, pore size 0.45 µm, Whatman) for 2 h using TE22 Mighty Small Transfer system (Hoefer Scientific) at 100 V (voltage constant). The membrane was then treated with or without phosphate-buffered saline (PBS) containing 0.4% PFA for 30 min at room temperature, followed by blocking for 1 h with 5% skim milk (Carnation) in Tris-buffered saline containing 0.1% Tween-20 (TBS-T). The membrane was then incubated for 1 h with a primary antibody in TBS-T containing 1% skim milk. As primary antibody, mouse monoclonal anti-α-syn antibodies 4D6 and LB509 (Santa Cruz Biotechnology) and rabbit monoclonal anti-phospho α-syn antibody EP1536Y (Epitomics, Burlingame, CA) were used at a dilution 1∶1,000, and rabbit polyclonal anti-actin antibody (Sigma) was also used at a dilution 1∶5,000. After washing with TBS-T containing 1% skim milk for 5 min three times, the membrane was incubated for 1 h with a secondary antibody, horseradish peroxidase-conjugated anti-mouse IgG or anti-rabbit IgG antibody (Santa Cruz Biotechnology), in TBS-T containing 1% skim milk. After washing with TBS-T for 10 min three times, protein bands on the membrane were detected by chemiluminescence method using ECL-Plus immunoblotting detection system (GE Healthcare).
Lee B.R, & Kamitani T. (2011). Improved Immunodetection of Endogenous α-Synuclein. PLoS ONE, 6(8), e23939.
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Publication 2011
2-Mercaptoethanol Actins Anti-Antibodies anti-IgG Antibodies, Anti-Idiotypic Carnation Chemiluminescence Electrophoresis Horseradish Peroxidase Immobilon P Immunoglobulins Membrane Proteins Methanol Mice, House Milk, Cow's Nitrocellulose Phosphates polyacrylamide gels polyvinylidene fluoride Proteins Rabbits Saline Solution Technique, Dilution Tetragonopterus Tissue, Membrane Tween 20
2
Embryo Protein Extraction and Western Blot
Cited 23 times
The samples were dissolved in 2 μl 2× SDS-sample buffer per embryo and incubated for 5 min at 95°C. No homogenisation was necessary since the cells dissolved rapidly in the buffer. After full speed centrifugation for 1 min in a microcentrifuge to remove insoluble particles, samples were loaded on a gel (per lane 10–15 embryos for a minigel, 15–30 embryos for large format gels). If not enough sample buffer was added, a slurry formed (presumably from DNA). If a semi-quantitative analysis is needed, care should be taken to load the same amounts of embryos in every lane. Since the volume of cells and remaining supernatant after deyolking is hard to control, we suggest to (1) either deyolk only the desired number of embryos and to load the whole sample or to (2) deyolk a sufficiently large number of embryos so that the volume of cells and remaining supernatant can be neglected against the large volume of added sample buffer.
Electrophoresis, blotting and detection was performed essentially as described in [13 ]. Briefly, 10% SDS-gels (10 × 10 cm) were run, semi-dry-blotted onto PVDF-membrane as described in the manual (Immobilon P, Millipore), stained for 5 min with Ponceau S, blocked 1 h in 5% nonfat dry milk in PBST (0.5% Tween-20 in PBS), incubated over night at 4°C with primary antibody in blocking buffer, washed 30 min with 4 changes of PBST, incubated 1 h with secondary antibody in blocking buffer and washed 30 min with 4 changes of PBST. Membranes were incubated for 5 min in ECL Plus (Amersham Biosciences) and emitted light was detected using a cooled CCD-camera (LAS-1000, Fujifilm).
Link V., Shevchenko A, & Heisenberg C.P. (2006). Proteomics of early zebrafish embryos. BMC Developmental Biology, 6, 1.
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Publication 2006
Antibodies, Blocking Cardiac Arrest Cells Centrifugation Electrophoresis Embryo Gels Immobilon P Immunoglobulins Light Milk, Cow's polyvinylidene fluoride ponceau S Tissue, Membrane Tween 20
3
Peptide-Specific Antibodies for PAD Enzymes
Cited 22 times
Peptides specific to PAD1 (H2N-CPKEPTRDAREAAPQ-COOH and H2N-ETCCRREVAE-COOH), PAD2 (H2N-EAEDNQTNAENVC-COOH and H2N-CNADACLEEKAADSSK-COOH) and the entire array (H2N-VETDVDYIAPQFQET-CONH2 and H2N-TQQADKLGQDVCTER-COOH) were used to generate antipeptide antibodies (Eurogentec). Immunofluorescence analysis was performed31 (link) using a Zeiss Axioscope 2 or Leica SP5 confocal microscope and analysed using Volocity software (Improvision). Images were processed using Adobe Photoshop CS. Western blotting was performed by low-voltage SDS-PAGE and wet transfer onto Immobilon-P PVDF (Millipore) according to the manufacturer's instructions; proteins were detected using the LI-COR Odyssey system for quantification against a tubulin loading control. Flow cytometry analysis was performed using the FACS-Calibur flow cytometer (Becton Dickenson)11 (link).
Dean S., Marchetti R., Kirk K, & Matthews K.R. (2009). A surface transporter family conveys the trypanosome differentiation signal. Nature, 459(7244), 213-217.
Publication 2009
Antibodies Flow Cytometry Immobilon P Immunofluorescence Microscopy, Confocal Peptides polyvinylidene fluoride Proteins SDS-PAGE Tubulin
4
Immunoblotting Protocol for Prion Protein Analysis
Cited 15 times
SDS-PAGE was performed with 10-well precast 1 mm 12% BisTris NuPAGE gels (NuPAGE gel electrophoresis system with MOPS buffer; Invitrogen, Breda, The Netherlands). Molecular weight markers used were MagicMark and SeeBlue Mark12 (Invitrogen). Sample volumes applied varied between 10 to 20 μl per lane, or 0.5 – 10 mg tissue equivalents (TE)/lane. Electrotransfer onto polyvinylidene difluoride membranes (PVDF, Immobilon-P; Millipore, Bedford, Mass.) and immunostaining were performed according to established procedures [67 (link), 68 (link)]. After electrotransfer, blots were blocked for 30 min with 5%skim milk protein in antibody incubation solution (25 mM Tris-HCl, 0.15 M NaCl, 2.7 mM KCl, 0.05% Tween20 at pH7.4). Primary antibodies were used at concentrations between 0.2–2 μg IgG/ml in antibody incubation solution. Secondary antibody used was rabbit anti-mouse immunoglobulinG conjugated to alkaline phosphatase (Dako, Glostrup, Denmark). Signal was developed with CDPStar by following the supplier's instructions (Tropix, Bedford, Mass.) and were recorded on photographic film, usually with exposure times between 1–45 min (Hyperfilm ECL; Amersham, Buckinghamshire, United Kingdom). Molecular weights were determined according to a method described [69 (link)]. To estimate glycoprofiles of PrPres i.e. the relative proportions of di-, mono-, and aglycosyl fraction, films were recorded with an Agfa Duoscan T200XL scanner and further processed with GelPro software (MediaCybernetics, Silver Spring, MD) from which calculation of mutual densities of the three protein bands was possible. In experiments to compare the relative affinity for ovine PrPres, antibodies were applied in concentration series on PVDFstrips from blots transferred from single well gels run with ovine scrapie infected brain stem homogenates varying between 1.25–20 mg tissue equivalents (TE).
Langeveld J.P., Jacobs J.G., Erkens J.H., Bossers A., van Zijderveld F.G, & van Keulen L.J. (2006). Rapid and discriminatory diagnosis of scrapie and BSE in retro-pharyngeal lymph nodes of sheep. BMC Veterinary Research, 2, 19.
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Publication 2006
Alkaline Phosphatase Antibodies Biological Markers Bistris Brain Stem Buffers Electrophoresis Gels Immobilon P Immunoglobulins Mice, House Milk Proteins morpholinopropane sulfonic acid polyvinylidene fluoride Proteins PrPSc Proteins Rabbits Scrapie SDS-PAGE Sheep Silver Sodium Chloride Tissue, Membrane Tissues Tromethamine Tween 20
5
Quantifying Exosomal Proteins by Western Blot
Cited 14 times
Protein-based quantitation of isolated exosomes was done using the protein DC assay kit as described earlier by us7 (link),34 (link). Subsequently, equal amount of exosomes (25 μg) collected from different isolation methods was denatured using 6X denaturation buffer at 95 °C for 10 min and then resolved on 12% SDS-Polyacrylamide gel by electrophoresis. Resolved proteins were transferred onto Immobilon-P PVDF membrane and then blocked by incubating in 5% skimmed milk to minimize non-specific binding of antibodies. Blocked blots were submerged with primary antibodies for CD9 and ARF-6 overnight, and subsequently washed three times (10 min each) with 1X Tris buffer saline with 0.1% Tween-20 (TBST) buffer followed by incubation with HRP-conjugated secondary anti-mouse (for CD9) and anti-rabbit (for ARF-6) antibodies. Unbound antibodies were removed by washing with 1X TBST buffer (3 × 10 min), and signal recorded using WestFemto maximum sensitivity substrate kit under Bio-Rad ChemiDoc Imager (Hercules, CA, USA).
Patel G.K., Khan M.A., Zubair H., Srivastava S.K., Khushman M., Singh S, & Singh A.P. (2019). Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications. Scientific Reports, 9, 5335.
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Publication 2019
Antibodies ARF6 protein, human Biological Assay Buffers Electrophoresis Exosomes Hypersensitivity Immobilon P isolation Mice, House Milk, Cow's polyacrylamide gels polyvinylidene fluoride Proteins Rabbits Saline Solution Tissue, Membrane Tromethamine Tween 20

Most recents protocols related to «Immobilon P»

1
Mass Spectrometry Glycoproteomic Protocol
Sodium borohydride, sodium chloride, Dowex cation-exchange resin (50W-X8), ammonium bicarbonate (ABC), TFA, Dulbecco’s PBS (DPBS), hydrochloric acid (HCl), and dl-DTT were purchased from Sigma–Aldrich. Ethanol (Reag. Ph. Eur) and bovine submaxillary mucin (BSM), type I-S, were purchased from Merck. Tandem mass tag (TMT)pro label reagents, 8 M guanidine hydrochloride, Dulbecco’s modified Eagle’s medium, 0.25% trypsin/EDTA, and fetal calf serum (FCS) were obtained from Thermo Fisher Scientific. Potassium hydroxide was obtained from Honeywell Fluka. Solid phase extraction bulk sorbent carbograph was obtained from Grace Discovery Sciences. HPLC SupraGradient acetonitrile (methyl cyanide [MeCN]) was obtained from Biosolve. Peptide N-glycosidase F (PNGase F) and complete EDTA-free protease inhibitor cocktail tablets were purchased from Roche Diagnostics. A 96-well PP filter plate was purchased from Orochem Technologies. MultiScreen HTS 96 multiwell plates (hydrophobic Immobilon-P polyvinylidene difluoride [PVDF] membrane) and 96-well PP microplate were obtained from Millipore.
Madunić K., Luijkx Y.M., Mayboroda O.A., Janssen G.M., van Veelen P.A., Strijbis K., Wennekes T., Lageveen-Kammeijer G.S, & Wuhrer M. (2023). O-Glycomic and Proteomic Signatures of Spontaneous and Butyrate-Stimulated Colorectal Cancer Cell Line Differentiation. Molecular & Cellular Proteomics : MCP, 22(3), 100501.
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Publication 2023
acetonitrile Acetonitriles ammonium bicarbonate BSM1 protein, Bos taurus Cation Exchange Resins Diagnosis Dietary Fiber Dowex Eagle Edetic Acid Endo-beta-N-Acetylglucosaminidase F Ethanol Fetal Bovine Serum High-Performance Liquid Chromatographies Hydrochloric acid Hydrochloride, Guanidine Immobilon P polyvinylidene fluoride potassium hydroxide Protease Inhibitors sodium borohydride Sodium Chloride Solid Phase Extraction Tissue, Membrane Trypsin
2
Western Blot Analysis of Signaling Pathways
A549 cells cultured in 2D monolayer or 3D aggregates were harvested and lysed in cell signaling lysis buffer (Merck Millipore) containing protease/phosphatase inhibitor cocktail (Cell Signaling Technology, Inc.). Protein quantification was performed using a Bradford assay kit (Bio-Rad Laboratories, Inc.). A total of 20 µg protein per lane was separated by 10% SDS-PAGE, electroblotted onto Immobilon-P Transfer Membranes (Merck Millipore) and blocked for 1 h at room temperature with 3% BSA (Sigma-Aldrich; Merck KGaA) in TBS-T (0.1% Tween-20). The membranes were then probed with the indicated primary antibodies: phosphorylated (p)-STAT3 (Y705; 1:250; rabbit, monoclonal; cat. no. 9145), STAT3 (1:3,000; rabbit, polyclonal; cat. no. 4904), p-Akt (1:250; rabbit, polyclonal; cat. no. 9271), Akt (1:1,000; rabbit, polyclonal; cat. no 9272), p-ERK (1:10,000; rabbit, polyclonal; cat. no. 9101), ERK (1:4,000; rabbit, polyclonal; cat. no. 9102), p-p38 (T180/Y182; 1:250; rabbit, polyclonal; cat. no. 9211), p38 (1:1,000; rabbit, polyclonal; cat. no. 9212), p-FAK (1:200; rabbit, monoclonal; cat. no. 8556), FAK (1:1,000; rabbit, polyclonal; cat. no. 3285), Mcl-1 (1:250; rabbit, polyclonal; cat. no. 4572), survivin (1:200; rabbit, polyclonal; cat. no. 2803), puma (1:500; rabbit, polyclonal; cat. no. 4976), cyclin D1 (1:250; rabbit, polyclonal; cat. no. 2922), cyclin D3 (1:1,000; mouse, monoclonal; cat. no. 2936), CDK2 (1:1,000; rabbit, monoclonal; cat. no. 2546) (all from Cell Signaling Technology, Inc.), MYLK (1:200; mouse, monoclonal; cat. no. sc-365352; Santa Cruz Biotechnology, Inc.) and GAPDH (1:50,000; rabbit, monoclonal; cat. no. ab190480; Abcam) at 4°C overnight. The membranes were washed and incubated for 1 h at room temperature in a 1:5,000 dilution of HRP-conjugated goat anti-rabbit (monoclonal; cat. no. 7074; Cell Signaling Technology, Inc.) or rabbit anti-mouse (polyclonal; cat. no. P0260; Dako; Agilent Technologies, Inc.) secondary antibodies. Visualization of the protein bands was performed with the SuperSignal™ West Pico PLUS Chemiluminescent Substrate (cat. no. 34580; Thermo Fisher Scientific) or SuperSignal™ West Femto Maximum Sensitivity Substrate (cat. no. 34096; Thermo Fisher Scientific, Inc.). Quantification of the bands was carried out by densitometry using ImageJ version 1.53k software (National Institutes of Health).
Keeratichamroen S., Sornprachum T., Ngiwsara L., Ornnork N, & Svasti J. (2023). p‑STAT3 influences doxorubicin and etoposide resistance of A549 cells grown in an in vitro 3D culture model. Oncology Reports, 49(4), 71.
Publication 2023
A549 Cells Antibodies Biological Assay Buffers CDK2 protein, human Cyclin D1 Cyclin D3 Densitometry GAPDH protein, human Goat Hypersensitivity Immobilon P Mice, House Mitogen-Activated Protein Kinase 3 MYLK protein, human Phosphoric Monoester Hydrolases Protease Inhibitors Proteins Puma Rabbits SDS-PAGE Staphylococcal Protein A STAT3 Protein Survivin Technique, Dilution Tissue, Membrane Tween 20
3
Western Blot Analysis of Protein Expression
The transfected cells or xenograft tumor tissue samples were rinsed with phosphate-buffered saline (PBS) and lysed in Pierce™ RIPA buffer (cat. no. 89900; Thermo Fisher Scientific, Inc.) with Halt™ phosphatase and protease inhibitor cocktail (cat. no. 1862495 and 1862209; Thermo Fisher Scientific, Inc.). The quantification of proteins in the cell lysate was performed using a BCA protein assay (cat. no. 23228; Thermo Fisher Scientific, Inc.). Equal amounts (20 µg/lane) of protein lysate were separated by electrophoresis on 8-12% polyacrylamide gels and transferred onto Immobilon®-P transfer membranes (cat. no. IPVH00010; MilliporeSigma). The blot membranes were incubated with 5% BSA solution at room temperature for 1 h and immunoblotted with specific antibodies (1:1,000 dilution) overnight at 4°C. Antibodies against ADAM12 (cat. no. ab28747), matrix metalloproteinase (MMP)2 (cat. no. ab37150) and MMP9 (cat. no. ab58803) were purchased from Abcam. Antibodies against E-cadherin (cat. no. #14472), Snail (cat. no. #3879), vimentin (cat. no. #5741), claudin-1 (cat. no. #4933), integrin α5 (cat. no. #4705), integrin β1 (cat. no. #9699), integrin β3 (cat. no. #13166), phosphorylated (p)-AKT (S473) (cat. no. #4060), p-phosphoinositide-dependent protein kinase 1 (PDK1) (S241) (cat. no. #3438), p-glycogen synthase kinase-3β (GSK-3β) (S9) (cat. no. #9323), total AKT (cat. no. #4691), total PDK1 (cat. no. #3062), total GSK-3β (cat. no. #9832) and Myc-tag (cat. no. #2278) were obtained from Cell Signaling Technology, Inc. Antibodies against β-tubulin (cat. no. sc-9104) and GAPDH (cat. no. sc-25778) were purchased from Santa Cruz Biotechnology, Inc. The blot membranes were washed four times with Tris-buffered saline-0.1% Tween-20 (TBS-T) and were then incubated with a horseradish peroxidase-conjugated secondary antibody (anti-rabbit, cat. no. #7074, anti-mouse, cat. no. #7076; Cell Signaling, Technology, Inc.) at 1:2,000 dilution for 1 h at room temperature. Amersham ECL Prime Western Blotting Detection Reagent (cat no. RPN2232SK; Cytiva) was used for blot development. Visualization of specific bands was obtained using the LAS-400 luminescent image analyzer (FUJIFILM Wako Pure Chemical Corporation). Semi-quantification of specific bands was performed using Multi-Gauge gel analysis software (version 3.0; FUJIFILM Wako Pure Chemical Corporation).
Oh H.H., Park Y.L., Park S.Y, & Joo Y.E. (2023). A disintegrin and metalloprotease 12 contributes to colorectal cancer metastasis by regulating epithelial-mesenchymal transition. International Journal of Oncology, 62(4), 50.
Publication 2023
1-Phosphatidylinositol 4-Kinase ADAM12 protein, human Antibodies Biological Assay Buffers Cadherins Cells Claudin-1 Electrophoresis GAPDH protein, human Glycogen Synthase Kinase 3 beta Helix (Snails) Horseradish Peroxidase Immobilon P Immunoglobulins Integrins Luminescence Matrix Metalloproteinase 2 MMP9 protein, human Mus Neoplasms Phosphates Phosphatidylinositols Phosphoric Monoester Hydrolases polyacrylamide gels Protease Inhibitors Protein Kinases Proteins Rabbits Radioimmunoprecipitation Assay Saline Solution Technique, Dilution Tissue, Membrane Tissues Tubulin Tween 20 Vimentin Xenografting
4
Western Blot Analysis of Pancreatic Cancer Cells
Panc-1 and MiaPaCa-2 cells were collected and lysed for 15 min on ice in RIPA buffer (Beyotime Institute of Biotechnology). Quantification of protein concentration was measured by bicinchoninic acid kit (Beyotime Institute of Biotechnology). A total of 30-50 µg protein was loaded per lane, separated by SDS-PAGE on 8-12% gels and transferred to 0.45-µm polyvinylidene difluoride (PVDF) membranes (Immobilon-P; MilliporeSigma). Subsequently, 5% skim milk (cat. no. 70166; Sigma-Aldrich; Merck KGaA) was used to block PVDF membranes for 1 h at room temperature. The membranes were incubated with primary antibodies overnight at 4°C. Horseradish peroxidase-conjugated secondary antibodies were then used to incubate membranes at room temperature for 1 h. Protein expression was detected using an enhanced chemiluminescence detection system (Thermo Fisher Scientific, Inc.). The following primary antibodies were used: Rabbit anti-human HNF1A (1:1,000; cat. no. ab96777; Abcam), 53BP1 (1:1,000; cat. no. ab87097; Abcam), mouse anti-human GAPDH (1:2,000; cat. no. abs830030; Absin Bioscience, Inc.) The secondary antibodies were goat anti-rabbit IgG-HRP (1:10,000; abs20002) and goat anti-mouse IgG-HRP (1:10,000; cat. no. abs20001) (both from Absin Bioscience, Inc.).
Xia R., Hu C., Ye Y., Zhang X., Li T., He R., Zheng S., Wen X, & Chen R. (2023). HNF1A regulates oxaliplatin resistance in pancreatic cancer by targeting 53BP1. International Journal of Oncology, 62(4), 45.
Publication 2023
anti-IgG Antibodies bicinchoninic acid Buffers Cardiac Arrest Cells Chemiluminescence GAPDH protein, human Gels Goat HNF1A protein, human Homo sapiens Immobilon P Milk, Cow's Mus polyvinylidene fluoride Proteins Rabbits Radioimmunoprecipitation Assay SDS-PAGE Staphylococcal Protein A Tissue, Membrane TP53BP1 protein, human
5
Whole Cell Protein Extraction and Analysis
2.5 OD600 units of yeast cells were harvested by centrifugation for 5min with 3.000 g at room temperature. Cells were resuspended in 200μL 0.1M NaOH followed by incubation at room temperature for 5min. Whole cell extracts were pelleted for 5min with 3.000 g at room temperature and the pellet was resuspended in 50μL 1x SDS sample buffer. Whole cell extracts were separated by electrophoresis, transferred onto polyvinylidene difluoride membranes (Immobilon®-P PVDF Membrane, Sigma-Aldrich) and blocked in 5% skimmed milk dissolved in 0.05% Tween/PBS for 1h at room temperature. Membranes were incubated with primary antibodies overnight at 4°C followed by washing in 0.1% Tween/TBS. Membranes were incubated with appropriate HRP- linked secondary antibodies at 25°C for 1h and washed thrice prior to signal detection. Membranes were developed by chemiluminescence using ECL reagent.
Weiβ M., Chanou A., Schauer T., Tvardovskiy A., Meiser S., König A.C., Schmidt T., Kruse E., Ummethum H., Trauner M., Werner M., Lalonde M., Hauck S.M., Scialdone A, & Hamperl S. (2023). Single-copy locus proteomics of early- and late-firing DNA replication origins identifies a role of Ask1/DASH complex in replication timing control. Cell Reports, 42(2), 112045.
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Publication 2023
Antibodies Buffers Cell Extracts Cells Centrifugation Chemiluminescence Electrophoresis Immobilon P Milk, Cow's polyvinylidene fluoride Signal Detection (Psychology) Tissue, Membrane Tweens Yeast, Dried

Top products related to «Immobilon P»

1
Immobilon p by Merck Group
Sourced in United States, Germany, United Kingdom, Japan, Ireland, Morocco, Switzerland, Sweden, France, Italy, Spain, China, Canada, Israel, Denmark
Immobilon-P is a polyvinylidene fluoride (PVDF) membrane designed for use in Western blotting applications. It provides a high-binding capacity for proteins and offers good mechanical strength and low background. The membrane is chemically stable and has a pore size of 0.45 μm.
2
Immobilon p membrane by Merck Group
Sourced in United States, Germany, United Kingdom, Japan, France, China, Morocco, Spain, Italy, Belgium, Canada, Australia
Immobilon-P membranes are a type of laboratory equipment used for protein transfer and immobilization. They are made of polyvinylidene fluoride (PVDF) material and are designed for efficient protein transfer during Western blotting and other related applications.
3
Immobilon p pvdf membrane by Merck Group
Sourced in United States, Germany, United Kingdom, Ireland, Australia, Italy, Morocco
Immobilon-P PVDF membrane is a polyvinylidene difluoride (PVDF) membrane designed for protein transfer and immobilization in Western blotting and other protein analysis applications. The membrane provides high protein-binding capacity and is compatible with a variety of detection methods.
4
Immobilon p transfer membrane by Merck Group
Sourced in United States, Germany, Japan, Spain, United Kingdom, China, Ireland, France
Immobilon-P transfer membrane is a polyvinylidene difluoride (PVDF) membrane used for the transfer and immobilization of proteins, DNA, and RNA from gels to solid supports. It provides a high-binding capacity and is suitable for use in a variety of blotting techniques, including Western blotting, Southern blotting, and Northern blotting.
5
Immobilon p polyvinylidene difluoride membrane by Merck Group
Sourced in United States, United Kingdom, Germany
Immobilon-P polyvinylidene difluoride (PVDF) membranes are a type of lab equipment used for protein and nucleic acid transfer and immobilization. They provide a stable, hydrophobic surface for the binding and retention of these biomolecules.
6
Protease inhibitor cocktail by Merck Group
Sourced in United States, Germany, China, United Kingdom, Italy, Japan, Sao Tome and Principe, France, Canada, Macao, Switzerland, Spain, Australia, Israel, Hungary, Ireland, Denmark, Brazil, Poland, India, Mexico, Senegal, Netherlands, Singapore
The Protease Inhibitor Cocktail is a laboratory product designed to inhibit the activity of proteases, which are enzymes that can degrade proteins. It is a combination of various chemical compounds that work to prevent the breakdown of proteins in biological samples, allowing for more accurate analysis and preservation of protein integrity.
7
Protease inhibitor cocktail by Roche
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Protease inhibitor cocktail is a laboratory reagent used to inhibit the activity of proteases, which are enzymes that break down proteins. It is commonly used in protein extraction and purification procedures to prevent protein degradation.
8
β actin by Merck Group
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β-actin is a protein that is found in all eukaryotic cells and is involved in the structure and function of the cytoskeleton. It is a key component of the actin filaments that make up the cytoskeleton and plays a critical role in cell motility, cell division, and other cellular processes.
9
Immobilon western chemiluminescent hrp substrate by Merck Group
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Immobilon Western Chemiluminescent HRP Substrate is a laboratory reagent used for the detection of proteins in Western blot analysis. It is a chemiluminescent substrate that reacts with horseradish peroxidase (HRP) enzyme, enabling the visualization of target proteins on a membrane.
10
Pierce bca protein assay kit by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, Australia, Switzerland, France, Italy, Canada, Spain, Japan, Belgium, Sweden, Lithuania, Austria, Denmark, Poland, Ireland, Portugal, Finland, Czechia, Norway, Macao, India, Singapore
The Pierce BCA Protein Assay Kit is a colorimetric-based method for the quantification of total protein in a sample. It utilizes the bicinchoninic acid (BCA) reaction, where proteins reduce Cu2+ to Cu+ in an alkaline environment, and the resulting purple-colored reaction is measured spectrophotometrically.

More about "Immobilon P"

Immobilon P is an innovative AI-powered platform developed by PubCompare.ai that empowers researchers to optimize their research protocols and enhance the reproducibility of their studies.
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