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Isoproterenol

Isoproterenol is a synthetic sympathomimetic amine with potent beta-adrenergic agonist activity.
It is used as a bronchodilator and to treat bradycardia and heart block.
Isoproterenol stimulates both beta-1 and beta-2 adrenergic receptors, leading to increased heart rate, myocardial contractility, and vasodilation.
It is also employed in research settings to induce cardiac hypertrophy and other cardiovascular effects in animal models.
Optimizing isoproterenol research can be challenging, but PubCompare.ai's AI-driven comparison and validation platform can help researchers locate the best protocols and products from literature, preprints, and patents, enhancing reproducibility and reliability.
Experince the power of data-driven decision making for your isoproterenol studies.

Most cited protocols related to «Isoproterenol»

Comprehensive Cardiac Molecular Analyses

Cited 19 times

Cell culture, luciferase reporter assays, quantitative reverse transcriptase–polymerase chain reaction, Northern blot analyses, Western blot analyses, and immunochemistry were performed according to routine protocols. Generation of miR-22 conventional and cardiac-specific knockout (KO) mice, isoproterenol (ISO) administration, and measurement of cardiac function by echocardiography are described in the Online Materials and Methods.

Huang Z.P., Chen J., Seok H.Y., Zhang Z., Kataoka M., Hu X, & Wang D.Z. (2013). MicroRNA-22 Regulates Cardiac Hypertrophy and Remodeling in Response to Stress. Circulation research, 112(9), 1234-1243.

Publication 2013

Biological Assay Cell Culture Techniques Echocardiography Heart Heart Function Tests Isoproterenol Luciferases Mice, Knockout Northern Blotting Reverse Transcriptase Polymerase Chain Reaction Western Blot

Glosensor Assay for Gα-Coupled Receptors

Cited 18 times

Cells were plated in 10-cm plates as previously described. Cells were transfected with plasmids encoding cDNA for the Glosensor reporter (Promega, Madison, WI), receptor, and Gα-subunit at a ratio of 2:1:1 (2 μg: 1 μg: 1μg). The next day, cells were plated in black, clear-bottom, 384-well white plates. After aspiration of the medium on the day of the assay, cells were incubated for 60 minutes at 37°C with 20 μL of 5 mM luciferin substrate (GoldBio, St. Louis, MO) freshly prepared in assay buffer. For Gαs activity, 10 μL of drugs were added using the FLIPR Tetra® liquid-handling robot and read after 15 minutes in a Spectramax luminescence plate reader (Molecular Devices, San Jose, CA) with a 0.5 second signal integration time. For Gαi activity, 10 μL of drugs were added for a 15-minute incubation period. Subsequently, 10 μL of isoproterenol (final concentration of 200 nM) was added and incubated for an additional 15-minute period before reading.

Olsen R.H., DiBerto J.F., English J.G., Glaudin A.M., Krumm B.E., Slocum S.T., Che T., Gavin A.C., McCorvy J.D., Roth B.L, & Strachan R.T. (2020). “TRUPATH, an Open-Source Biosensor Platform for Interrogating the GPCR Transducerome”. Nature chemical biology, 16(8), 841-849.

Publication 2020

Biological Assay Buffers Cells DNA, Complementary Isoproterenol Luciferins Luminescence Medical Devices Pharmaceutical Preparations Plasmids Promega Protein Subunits Tetragonopterus

Measuring Adipocyte Lipolysis

Cited 16 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2014

Adipocytes Adrenergic Agents Cells Colforsin Glycerin Isoproterenol Lipolysis

Glosensor Assay for Gα-Coupled Receptors

Cited 13 times

Publication 2020

Biological Assay Buffers Cells DNA, Complementary Isoproterenol Luciferins Luminescence Medical Devices Pharmaceutical Preparations Plasmids Promega Protein Subunits Tetragonopterus

Cardiac Differentiation of NKX2-5 and MYBPC3 hiPSCs

Cited 13 times

H3 NKX2-5^eGFP/w hESCs or M1 NKX2-5^eGFP/w hESCs as previously generated (Elliott et al., 2011 (link)) were maintained on mouse embryonic fibroblasts and passaged using TrypLE select (Life Technologies). The generation of transgene-free hiPSCs from skin fibroblasts of one healthy male donor (LUMC0004iCtrl [Con1]) and three patients each with a c.2373dupG mutation in MYBPC3 (LUMC0033iMyBPC [HCM1], LUMC0034iMyBPC [HCM2], and LUMC0035iMyBPC [HCM3]) was previously reported (Dambrot et al., 2014 (link)). A second transgene-free control hiPSC line (LUMC0047iCtrl [Con2]) generated from another healthy male donor was included in this study. hiPSCs were maintained on Matrigel (growth factor reduced; Corning 354230) in mTeSR1 medium (Stem Cell Technologies) and passaged with 1 mg/ml Dispase (Life Technologies). NKX2-5^eGFP/w hiPSCs (R.P.D. and C.L.M., unpublished data) were maintained in Essential 8 medium (Life Technologies) and differentiated as previously described (van den Berg et al., 2015 ).
Cardiac differentiation was induced from monolayer cultures on Matrigel in a serum-free medium (BSA, polyvinyl alcohol, essential lipids [BPEL]) as described in the Supplemental Information. Contracting cultures were dissociated on day 13 and replated on Matrigel-coated 24-well plates. The following experimental factors were added on day 16, refreshed on day 20, and measured on day 21: 100 ng/ml Long R3 IGF-1 (in the main text: IGF-1), 1 μM SAG (Millipore), 1 μM dexamethasone, 100 nM triiodothyronine hormone, 10 μM phenylephrine, 1 μM isoproterenol, and 1-1000 nM norepinephrine. Unless otherwise stated, all factors were obtained from Sigma-Aldrich.
The composition of the defined cardiomyocyte medium used in the MYBPC3 shRNA experiment can be found in the Supplemental Information.

Birket M.J., Ribeiro M.C., Kosmidis G., Ward D., Leitoguinho A.R., van de Pol V., Dambrot C., Devalla H.D., Davis R.P., Mastroberardino P.G., Atsma D.E., Passier R, & Mummery C.L. (2015). Contractile Defect Caused by Mutation in MYBPC3 Revealed under Conditions Optimized for Human PSC-Cardiomyocyte Function. Cell Reports, 13(4), 733-745.

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Publication 2015

Dexamethasone dispase Embryo Fibroblasts Growth Factor Heart Hormones Human Embryonic Stem Cells Human Induced Pluripotent Stem Cells IGF1 protein, human Isoproterenol Liothyronine Lipids Males matrigel Mice, Laboratory Mutation Myocytes, Cardiac Norepinephrine Patients Phenylephrine Polyvinyl Alcohol Serum Short Hairpin RNA Skin Stem Cells Tissue Donors Transgenes

Most recents protocols related to «Isoproterenol»

Investigating β2AR-βarr Binding Interactions

Not available on PMC !

Example 1

This example demonstrates that the binding interaction of βarr with the β2-adrenergic receptor (β2AR).

The binding of βarr to GPCRs is mainly initiated through an interaction with the phosphorylated receptor C terminus, and conformational changes induced in βarr by this interaction promote coupling to the receptor TM core, as shown in FIG. 1. Co-immunoprecipitation experiments confirmed that heterotrimeric Gs protein, but not βarr1, can interact with purified non-phosphorylated β2-adrenergic receptor (β2AR), as shown in FIG. 2A.

To verify that this apparent lack of interaction with βarr is not simply due to poor complex stability, two assays capable of detecting complex formation in situ were performed. First, competition radioligand binding was used to measure the allosteric effects of transducers on ligand binding to the receptor. As described by the ternary complex model, first for G proteins and later for βarrs, ligand-induced changes in receptor conformation enhance the binding and affinity of transducers, which reciprocally increase ligand affinity by stabilizing an active receptor state (De Lean A, et al. (1980) J Biol Chem 255(15):7108-7117., Gurevich V V, et al. (1997) J Biol Chem 272(46):28849-28852). When wild-type (WT) β2AR was reconstituted in high-density lipoprotein (HDL) particles to mimic a cellular membrane environment (Denisov I G & Sligar S G (2016) Nat Struct Mol Biol 23(6):481-486), G protein enhanced the affinity of the full agonist isoproterenol for non-phosphorylated HDL-β2AR by nearly 1000-fold, as expected, but βarr1 had no effect even at micromolar concentrations, as shown in FIG. 2B.

Second, to directly monitor β2AR conformational changes associated with activation, the C265 at the cytoplasmic end of TM6 was labeled with monobromobimane, an environmentally sensitive fluorophore. Receptor activation leads to an outward movement of TM6 that places the bimane label in a more solvent-exposed position, causing a decrease in fluorescence and a shift in λmax (Yao X J, et al. (2009) Proc Natl Acad Sci USA 106(23):9501-9506). Indeed, isoproterenol reduced β2AR-bimane fluorescence compared to control (DMSO), and addition of Gs but not βarr1 further attenuated fluorescence, as shown in FIG. 2C.

The results of this example demonstrate that non-phosphorylated β2AR fails to form a productive interaction with βarr.

US11866482B2. System and method for homogenous GPCR phosphorylation and identification of beta-2 adrenergic receptor positive allosteric modulators (2024-01-09). Duke University [US]. Inventors: Robert J. Lefkowitz [US], Seungkirl Ahn [US], Biswaranjan Pani [US], Alem W. Kahsai [US], Laura Wingler [US], Dean Staus [US].

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Patent 2024

Adrenergic Agents beta-2 Adrenergic Receptors Biological Assay Co-Immunoprecipitation Cytoplasm Fluorescence GTP-Binding Proteins high density lipoprotein receptors High Density Lipoproteins Homozygote Isoproterenol Ligands monobromobimane Movement Phosphorylation Plasma Membrane Proteins Solvents Sulfoxide, Dimethyl Transducers

Isoprenaline-Induced LMS Contraction

Culture medium was refreshed after 1, 24 and subsequently every 48 h by replacing 1.6 of 2.4 mL in the BMCC. Preload was readjusted to 1 mN at every medium exchange. Isoprenaline was added after each medium exchange, maintaining the final concentration constant at 1 µM. Without isoprenaline enhancement, LMS contraction force quenched to a minimum level. Contraction force was measured using dedicated MyoDish Software^{3 (link)}.

Amesz J.H., de Groot N.M., Langmuur S.J., Azzouzi H.E., Tiggeloven V.P., van Rooij M.M., Knops P., Bogers A.J, & Taverne Y.J. (2023). Biomimetic cultivation of atrial tissue slices as novel platform for in-vitro atrial arrhythmia studies. Scientific Reports, 13, 3648.

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Publication 2023

Isoproterenol

Isolation and Characterization of Murine Fibroblastic Reticular Cells

FRC cell lines were established from peripheral LNs by long-term culturing as described previously^{74 (link)} with minor modifications. In brief, LNs from 8-week-old C57BL/6 N mice were dissected and disrupted using two 25 G needles before enzymatic digestion with DMEM medium containing 3.5 mg/ml Collagenase D and 40 μg/ml DNase I at 37 °C for 30 min with agitation⁷⁵. The mixture was then filtered through a 70 μm cell strainer and centrifuged at 300 g for 5 min at 4 °C. The cell pellet was resuspended and cultured in DMEM medium (supplemented with 10% FBS and 1% Penicillin/Streptomycin) (5% CO₂, 37 °C). After 24 h, non-adherent cells were removed, and fresh medium was added to continue culturing until cells reached confluence. Adherent-stromal cells were then trypsinized, and triple-stained with antibodies to identify FRCs: Cd45 Pacific blue (1:100), Cd31 PE (1:100) and gp38 APC (1:100). FRCs were sort-purified using a MoFlo Optical Bench Sorter (Beckman Coulter) to achieve a purity of ≥95%. The sorted cells were immediately cultured in DMEM medium for expansion, and then seeded into 60 mm dishes in a density of 3 × 10⁵ per well to grow until confluence, followed by starvation for overnight and treatment with 10 μM isoproterenol (Sigma-Aldrich), 5 μM forskolin (Sigma-Aldrich), and 5 μM PKA inhibitor H89 dihydrochloride hydrate (Sigma-Aldrich) for 8 h. Culture media were collected to determine the concentration of IL-33 using a mouse IL-33 immunoassay kit (Immunodiagnostics Limited) or LDH using a CyQUANT LDH Cytotoxicity fluorescent assay kit (Thermo Fisher Scientific).

Cheong L.Y., Wang B., Wang Q., Jin L., Kwok K.H., Wu X., Shu L., Lin H., Chung S.K., Cheng K.K., Hoo R.L, & Xu A. (2023). Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice. Nature Communications, 14, 1213.

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Publication 2023

Antibodies Biological Assay Cell Lines Cells Colforsin Collagenase Culture Media Cytotoxin Deoxyribonuclease I Digestion Enzymes Hyperostosis, Diffuse Idiopathic Skeletal IL33 protein, human Immunoassay Immunodiagnosis Isoproterenol Mice, Inbred C57BL Mus Needles Penicillins PKA inhibitor Streptomycin Stromal Cells Vision

Transgenic Mice for Cardiac Studies

The BAC transgene Tbx5^CreERT2 was constructed from the BAC clone RP23-267B15^{70 (link)} by replacement of exon 2 of Tbx5 with a CreERT2 cassette at the first methionine of the open reading frame in EL250 cells^{23 (link),71 (link)}. The BAC-Tbx5^CreERT2 transgenes were crossed with Rosa26R^eYFP/eYFP transgenic mice (B6.129×1-Gt(ROSA)26Sortm1(EYFP)Cos/J) from Jackson laboratories stock# 006148, in order to produce Tbx5^CreERT2/Rosa26R^eYFP/eYFP mice employed in this study. The Tbx5^CreERT2/+/Rosa26R^eYFP/+/Rosa26R^iDTR/+ was created using the tamoxifen-induced Rosa26R^iDTR/iDTR transgene^{72 (link)} (Jackson laboratories stock# 007900) provided by the Klinakis lab (BRFAA).
All animal work has been approved by the BRFAA ethics committee and the Attica Veterinary Department (Animal Licence; 60876/23-1-20). All animals used were 2–3 months of age upon the time of ischemia/reperfusion (I/R) or isoproterenol administration experiments following relevant inclusion/exclusion guideline criteria^{73 (link)}.

Siatra P., Vatsellas G., Chatzianastasiou A., Balafas E., Manolakou T., Papapetropoulos A., Agapaki A., Mouchtouri E.T., Ruchaya P.J., Korovesi A.G., Mavroidis M., Thanos D., Beis D, & Kokkinopoulos I. (2023). Return of the Tbx5; lineage-tracing reveals ventricular cardiomyocyte-like precursors in the injured adult mammalian heart. NPJ Regenerative Medicine, 8, 13.

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Publication 2023

Animals Clone Cells Ethics Committees Exons Ischemia Isoproterenol Methionine Mice, Laboratory Mice, Transgenic Reperfusion Rosa Tamoxifen Transgenes

Primary Airway Cell Culture Protocol

PBECs were cultured with keratinocyte serum-free medium (KSFM, Gibco) containing 0.2 ng/ml epidermal growth factor (EGF) and 25 µg/ml pituitary bovine extract (BPE) supplemented with 1 µM isoproterenol, until confluence on fibronectin/collagen pre-coated plates.

Wu D.D., Lau A.T., Xu Y.M., Reinders-Luinge M., Koncz M., Kiss A., Timens W., Rots M.G, & Hylkema M.N. (2023). Targeted epigenetic silencing of UCHL1 expression suppresses collagen-1 production in human lung epithelial cells. Epigenetics, 18(1), 2175522.

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Publication 2023

Bos taurus Collagen Culture Media Epidermal growth factor FN1 protein, human Isoproterenol Keratinocyte Serum

Top products related to «Isoproterenol»

Isoproterenol by Merck Group

Sourced in United States, Germany, Japan, United Kingdom, Sao Tome and Principe, Canada, France, Denmark, Italy, Sweden

Isoproterenol is a synthetic catecholamine used as a laboratory reagent. It acts as a non-selective beta-adrenergic agonist, stimulating both beta-1 and beta-2 adrenergic receptors. Isoproterenol is commonly used in research applications to study cardiovascular and respiratory function.

Isoprenaline by Merck Group

Sourced in United States, United Kingdom, Germany, France, Sao Tome and Principe

Isoprenaline is a laboratory reagent used as a research tool in various scientific applications. It functions as a synthetic catecholamine and is commonly utilized in experiments related to physiology, pharmacology, and biochemistry. The core function of Isoprenaline is to act as a non-selective beta-adrenergic agonist, which can be used to study the effects of beta-adrenergic receptor activation in various experimental models.

Free glycerol reagent by Merck Group

Sourced in United States, Germany, Denmark, Japan, Spain, Holy See (Vatican City State)

The Free Glycerol Reagent is a laboratory product designed to measure the concentration of free glycerol in a sample. It provides a reliable and accurate method for determining the level of free glycerol, which is a useful metric in various applications.

Insulin by Merck Group

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Insulin is a lab equipment product designed to measure and analyze insulin levels. It provides accurate and reliable results for research and diagnostic purposes.

Isoproterenol iso by Merck Group

Sourced in United States

Isoproterenol (ISO) is a synthetic catecholamine compound used as a laboratory reagent. It is a non-selective beta-adrenergic agonist, primarily stimulating both beta-1 and beta-2 adrenergic receptors. ISO is commonly used in research applications to study the physiological effects of beta-adrenergic receptor activation.

Propranolol by Merck Group

Sourced in United States, Germany, United Kingdom, China, France, Macao, Sao Tome and Principe, Spain

Propranolol is a laboratory reagent used as a β-adrenergic receptor antagonist. It is commonly used in research applications to study the role of the sympathetic nervous system.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Isoproterenol by Bio-Techne

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Isoproterenol is a laboratory reagent used as a non-selective beta-adrenergic agonist. It is commonly used in research applications to study the effects of beta-adrenergic receptor activation.

Dexamethasone (dex) by Merck Group

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Dexamethasone is a synthetic glucocorticoid medication used in a variety of medical applications. It is primarily used as an anti-inflammatory and immunosuppressant agent.

Carbachol by Merck Group

Sourced in United States, United Kingdom, Germany, Sao Tome and Principe, Italy, Australia, Japan, Hungary, Brazil, France

Carbachol is a chemical compound that acts as an acetylcholine receptor agonist. It is commonly used in laboratory settings as a research tool to study the effects of acetylcholine on various biological systems.

What are some common challenges in optimizing isoproterenol research?

Optimizing isoproterenol research can be challenging due to the complex pharmacological effects of this beta-adrenergic agonist. Researchers need to carefully consider factors like dosage, administration route, and timing to achieve the desired cardiovascular responses in animal models. Inconsistencies in experimental protocols can lead to variability in results and poor reproducibility. This is where PubCompare.ai can help by enabling more efficient screening of protocol literature and leveraging AI to pinpoint critical insights that guide researchers towards the most effective isoproterenol protocols for their specific needs.

How can PubCompare.ai assist in optimizing isoproterenol research?

PubCompare.ai's AI-driven comparison and validation platfrom can be invaluable for researchers working with isoproterenol. The platfrom allows you to more efficiently screen protocol literature from published studies, preprints, and patents to identify the most effective approaches. The AI-powered analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy in your isoproterenol studies. This data-driven decision making can help enhance the reliability and reproducibility of your research, leading to more meaningful and impactful findings.

Are there different variations or types of isoproterenol that researchers should be aware of?

Yes, there are several variations and formulations of isoproterenol that researchers should be familair with. In addition to the widely used hydrochloride salt form, there is also an isoproterenol sulfate formulation. The choice of isoproterenol type can impact factors like solubility, stability, and potency, which are important considerations when designing animal studies. Researchers should carefully review the literature and consult with PubCompare.ai to determine the most suitable isoproterenol variation for their specific experimental protocols and research objectives.

What are some of the key applications of isoproterenol in research?

Isoproterenol has a wide range of applications in research settings. It is commonly used as a bronchodilator and to treat conditions like bradycardia and heart block. Importantly, isoproterenol is also employed to induce cardiac hypertrophy and other cardiovascular effects in animal models, making it a valuable tool for studying the underlying mechanisms and potential therapeutic interventions for heart disease. By leveraging PubCompare.ai's platform, researchers can more easily identify the most effective isoproterenol protocols to address their specific research goals and questions.

More about "Isoproterenol"

Isoproterenol, also known as isoprenaline or Isuprel, is a synthetic catecholamine and potent beta-adrenergic agonist.
It acts as a non-selective agonist, stimulating both beta-1 and beta-2 adrenergic receptors.
This leads to increased heart rate, myocardial contractility, and vasodilation, making it a valuable tool in the treatment of bradycardia, heart block, and as a bronchodilator for respiratory conditions like asthma.
Researchers commonly use isoproterenol in animal models to induce cardiac hypertrophy and study other cardiovascular effects.
However, optimizing isoproterenol research can be challenging.
Factors like dosage, administration route, and timing can significantly impact the results.
Fortunately, AI-driven platforms like PubCompare.ai can help researchers identify the best protocols and products from literature, preprints, and patents, enhancing the reproducibility and reliability of their isoproterenol studies.
In addition to isoproterenol, related compounds like isoprenaline, free glycerol reagent, insulin, propranolol (a beta-blocker), and dexamethasone (a corticosteroid) may also be relevant in cardiovascular and metabolic research.
By leveraging the power of data-driven decision making, researchers can optimize their use of these compounds and gain deeper insights into the underlying mechanisms and therapeutic potential.
Experience the benefits of AI-powered research optimization for your isoproterenol and related studies.