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Ketoprofen

Ketoprofen is a propionic acid derivative with anti-inflammatory, analgesic, and antipyretic properties.
It is used to treat rheumatoid arthritis, osteoarthritis, and other musculoskeletal conditions.
Ketoprofen inhibits prostaglandin synthesis by blocking the cyclooxygenase enzyme, leading to reduced inflammation and pain.
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Most cited protocols related to «Ketoprofen»

rAAVs (AAV2/1; synapsin-1 promoter) were injected into the primary somatosensory cortex (S1) of 2–3 week old C57Bl/6Crl wild-type mice. Two weeks after injection, mice were anaesthetized with 2% isoflurane, and a 1.5mm circular craniotomy was performed over the injection site as previously described 43 (link). Cells were recorded with a patch pipette containing (in mM): 10.0 KCl, 140 K-gluconate, 10.0 HEPES, 2.0 MgCl2, 2.0 CaCl2, 0.05 Alexa 594, pH 7.25, 290 mOsm. For recording and stimulation a MultiClamp 700B amplifier (Molecular Devices, Sunnyvale, California) was used. In whole cell mode, action potentials were evoked by 2–5 ms long current injections; in cell attached mode currents up to 100 nA were necessary. The Ti:Sapphire laser (Mai Tai, Spectro-Physics, CA) was tuned to 910 nm for GCaMP3 imaging. Fluorescence images were simultaneously acquired using a custom-built, two-photon laser-scanning microscope equipped with a 40X, 0.8 NA objective (Olympus, Tokyo, Japan). Frame scans were acquired at 15 Hz (256×32 pixels) for a period of 3 seconds.
For imaging awake, head-fixed running mice, virus injection and surgery were identical to the anesthetized condition, except that the injection and craniotomy were performed over the primary whisker and forelimb motor area (M1). In addition, local (Marcaine) and general (Buprenorphine, 0.1mg/kg IP and Ketoprofen, 5mg/kg SC) anesthetics were administered. After full recovery on a heating pad the animals were head restrained, but allowed to run freely on a linear treadmill. Action potentials were recorded using a loose-seal cell attached configuration with patch pipettes filled with buffer (in mM: 125 NaCl, 2.5 KCl, 25.0 glucose, 10.0 HEPES, 2.0 CaCl2, 2.0 MgSO4, 0.05 Alexa 594; pH 7.4, 285 mOsm), and signals were amplified using a MultiClamp 700B (Molecular Devices, Sunnyvale, California). To confirm the identity of recorded neurons, each recording was terminated by breaking into the cell and filling with red pipette solution. During the imaging sessions the animals were kept alert by sporadic acoustic stimuli (clapping) or by presenting a pole or mild air puffs to the whisker field. Images were acquired at frame rates of 4–8 Hz at a resolution of 256×512 pixels using a 16X, 0.8 NA water immersion objective (Nikon USA, Lewisville, TX). All images acquired while awake were corrected for movement artifacts using the ImageJ plug-in TurboReg (http://bigwww.epfl.ch/thevenaz/turboreg/). ΔF/F was calculated by subtracting the baseline fluorescence level (F0, 35th percentile of total fluorescence) from the actual fluorescence level and normalized to F0.
Publication 2009
Acoustics Action Potentials Alexa594 Anesthetics Animals Buffers Buprenorphine Cells Craniotomy Fluorescence Forelimb gluconate Glucose Head HEPES Isoflurane Ketoprofen Laser Scanning Microscopy Magnesium Chloride Marcaine Medical Devices Mice, Inbred C57BL Motor Cortex Movement Mus Neoplasm Metastasis Neurons Operative Surgical Procedures Phocidae Radionuclide Imaging Reading Frames Sapphire Sodium Chloride Somatosensory Cortex, Primary Submersion Sulfate, Magnesium Synapsin I Vibrissae Virus
For intracranial injections, post-natal day 14–40 mice were anesthetized with isofluorane and placed into a stereotaxic apparatus. For co-injections, viruses were mixed at ratios that were adjusted empirically to achieve approximately equal expression levels of GFP and mCherry/TdTomato fluorophores. Virus was delivered at 100nl/min using a Microinject system (WPI). Striatum was targeted by directing the needle approximately 0.9mm anterior and 2.4mm lateral from Bregma and 2.1mm from the pial surface. Cortical injections were targeted using the same anterior–posterior and medial-lateral coordinates, but the needle was directed 0.4mm from the pial surface. Coordinates were adjusted slightly by age and 300–1000nl were injected bilaterally. Following injections and wound closure, mice received ketoprofen (10 mg/kg) for analgesia and were returned to home cages for 3–4 weeks. All surgical procedures, animal handling, and euthanasia were carried out in accordance with federal guidelines and were approved by the Harvard Medical School Institutional Animal Care and Use Committee.
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Publication 2012
Animals Euthanasia Institutional Animal Care and Use Committees Ketoprofen Kidney Cortex Management, Pain Mice, Laboratory Needles Operative Surgical Procedures Striatum, Corpus tdTomato Virus Wounds
All animal studies were approved by the Institutional Animal Care and Use Committee of Vanderbilt University. Briefly, hypertension was induced in male wild-type mice (C57BL/6; Jackson Laboratories) at 3 months of age via chronic infusion of angiotensin-II (Ang II; Sigma-Aldrich) at a continuous rate of 490 ng/kg/min for 2 or 4 weeks using an osmotic mini-pump (Alzet). This pump was implanted subcutaneously on the flank under sterile conditions during a brief surgery in which Ketoprofen (5 mg/kg) was used for pre-anesthesia and ketamine (100mg/kg) and xylazine and 10 (mg/kg) was used for anesthesia. Blood pressure was measured every hour over the up to 4-week study period via an indwelling catheter and telemetry system (Figure S1). At the prescribed endpoint, the mice were euthanized by CO2 inhalation and the descending thoracic aorta was excised from the left subclavian artery to the third pair of intercostal branches. Age-matched control vessels were obtained similarly, but following a Sham procedure wherein the implanted mini-pumps released normal saline rather than Ang II. Additional details can be found elsewhere.1 (link)The Online Data Supplement describes established methods for biaxial mechanical testing, quantitative histology, and computing wall stress, material stiffness, (an intrinsic property of the wall), structural stiffness (which depends on material stiffness and geometry), and stored energy (http://hyper.ahajournals.org).
Publication 2016
Anesthesia Angiotensin II Animals Blood Pressure Blood Vessel Dietary Supplements High Blood Pressures Indwelling Catheter Inhalation Institutional Animal Care and Use Committees Ketamine Ketoprofen Males Mice, House Normal Saline Operative Surgical Procedures Osmosis Sterility, Reproductive Subclavian Artery Telemetry Thoracic Aorta Xylazine
As previously reported, the W/+Emx1-Cre mice develop spontaneous tonic–clonic seizures at 4 weeks of age, and 75% succumb to seizure-induced death by 2 months.25 (link) D/+ mice have a milder phenotype, with seizure onset at 8 to 16 weeks and only 50% lethality by 6 months.27 (link) Custom headsets (Plastics One, Roanoke, VA) were implanted in 4- to 5-week-old W/+Emx1-Cre and 8- to 10-week-old D/+ mice using approved surgical techniques as previously described by our laboratories.18 (link),25 (link) Briefly, electroencephalographic (EEG) electrodes were placed over the parietal lobe unilaterally, and electrocardiographic (ECG) electrodes were placed in an approximate lead II configuration. In a subset of D/+ mice, ECG leads were replaced with leads that extended to contact the diaphragm, so as to record diaphragm electromyogram (EMG) as previously described.30 (link) A reference electrode was placed over the cerebellum. Normal saline (8ml/kg, intraperitoneal [i.p.]) and ketoprofen (5mg/kg, i.p.) were given postoperatively. Mice were monitored, and additional ketoprofen was administered every 24 hours as needed. After recovery from surgery (minimum of 4 days), mice underwent continuous monitoring of EEG, ECG/EMG, and breathing in custom-designed plethysmography chambers that comply with guidelines for continuous housing described in the Guide for the Care and Use of Laboratory Animals,31 and as described previously.18 (link) The same techniques were used for recording seizures from W/+EIIA-Cre mice at postnatal age 15 days. However, because W/+EIIA-Cre mice were used prior to weaning, due to their early lethality, they could only be allowed 24 hours to recover from surgery, because degradation of ECG signals was common due to parental grooming.
Audiogenic seizures were induced in W/+EIIA-Cre and D/+ mice with a 15kHz pure tone (~80–100dB), generated using Tone Generator software (NCH Software, Canberra, Australia), amplified with a Kinter K3118 stereo amplifier (Kinter USA, Amazon.com), and converted to sound using a small 3W speaker lowered into the plethysmography chamber. For mechanical ventilation experiments, a sonicator (Branson 200 ultrasonic cleaner) was placed next to the mouse cage and turned on to induce audiogenic seizures (see Video S5).
PTZ-induced seizures were generated by i.p. injection of 90mg/kg PTZ, which resulted in 100% lethality, as previously reported.32 (link),33 (link) PTZ (Sigma-Aldrich, St Louis, MO) was dissolved in normal sterile saline on the day of administration.
Publication 2021
Animals, Laboratory Cerebellum Clonic Seizures, Tonic Electrocardiograph Electroencephalography Electromyography Ketoprofen Mechanical Ventilation Mice, Laboratory Normal Saline Operative Surgical Procedures Parent Parietal Lobe Phenotype Plethysmography Reflex Epilepsy, Audiogenic Seizures Sound Sterility, Reproductive Ultrasonics Vaginal Diaphragm
Six- to 8-week-old male C57BL/6 mice served as recipients of donor trachea obtained from C57Bl/6 female mice of the same age and similar weight (Fig. 1).
Tracheas were harvested from female donors using an aseptic surgical technique. Mice were euthanized using a cocktail of ketamine (100 mg/kg), xylazine (10 mg/kg), and ketoprofen (5 mg/kg) via intraperitoneal injection. After obtaining a deep anesthetic plane, the surgical site was shaved and disinfected with alcohol and povidone iodine. A midline incision was made from the chin to the center of the chest, and subcutaneous fat pads were removed from the field. The strap muscles surrounding the trachea were separated and the trachea was dissected circumferentially. A 5-mm length of trachea was harvested and maintained in fresh phosphate-buffered saline (PBS) until implant.
Recipient male mice were prepared for transplant using the previously described surgical process for female mice. The native trachea was resected and replaced with donor female trachea using interrupted sutures of 9–0 nylon (AROS Surgical Instruments, Newport Beach, CA). After the transplanted trachea was secured in an air-tight fashion, the strap muscles were reapproximated, and the midline incision was closed using running stitches with 6–0 Vicryl suture (Ethicon, Somerville, NJ).
Postoperative care was the same as our previous study.4 Animals were evaluated daily during the first 2 weeks monitoring for weight loss >20%, stridor, and respiratory distress. Animals meeting the criteria were recommended for humane euthanasia prior to the planned endpoint and removed from the study.
Publication 2020
Anesthetics Animals Asepsis Chest Chin Donors Ethanol Euthanasia Females Injections, Intraperitoneal Ketamine Ketoprofen Males Mice, House Mice, Inbred C57BL Muscle Tissue Nylons Operative Surgical Procedures Pad, Fat Phosphates Postoperative Care Povidone Iodine Respiratory Rate Saline Solution Stridor Surgical Instruments Sutures Trachea Vicryl Xylazine

Most recents protocols related to «Ketoprofen»

Retention times of the analytes were measured with Shimadzu HPLC system on the CHIRALPAK®HAS stationary phase (50 × 3 mm, 5 μm, Chiral Technologies, DAICEL Group, Europe SAS, France). The mobile phase A consisted of 50 mM aqueous ammonium acetate buffer (pH 7.4) and phase B of 2-propanol according to Valko et al.65 (link) Analysis was performed at prolonged 1 mL min−1 flow rate in the linear gradient. Retention capacity factors (k′) were calculated by using DMSO or a substance with 0% HAS binding for systems' dead time (Rt0). The system was calibrated by injecting the reference compounds: acetylsalicylic acid (CAS 69-72-7), betamethasone (CAS 378-44-9), budesonide (CAS 5133-22-3), carbamazepine (CAS 298-46-4), cimetidine (CAS 51481-61-9), ciprofloxacin (CAS 85721-33-1), indomethacin (CAS 53-86-1), isoniazid (CAS 54-85-3), metronidazole (CAS 443-48-1), nicardipine (CAS 55985-32-5), nizatidine (CAS 76963-41-2) and warfarin (CAS 81-81-2) obtained from Sigma-Aldrich, diclofenac (CAS 15307-86-5) from EMD Chemicals Inc., flumazenil (CAS 78755-81-4) from ABX and ketoprofen (CAS 22071-15-4) from LKT Labs. The logarithmic capacity factors of the references' Rt (log(k′)) on the HSA column were plotted against the %PPB values from literature. The slope and the intercept were used to convert the log(k′) of the compounds (6a, c, f, h, m–o) to %PPB using the regression equation.66 (link)
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Publication 2023
ammonium acetate Aspirin Betamethasone Budesonide Buffers Carbamazepine Cimetidine Ciprofloxacin Diclofenac Flumazenil High-Performance Liquid Chromatographies Indomethacin Isoniazid Ketoprofen Metronidazole Nicardipine Nizatidine Propanols Retention (Psychology) Sulfoxide, Dimethyl Warfarin

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Publication 2023
Analgesics Animals Animals, Laboratory Antibiotics Cefazolin Donors Institutional Animal Care and Use Committees Ketoprofen Mice, House Mice, Inbred BALB C

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Publication 2023
Allografts Buffers Cefazolin Erythema Face IL10 protein, human Ketoprofen Mice, House Necrosis Saline Solution
The porcine models were kept fasting for 12 hours before administration of general anesthesia. Anesthesia was achieved using tiletamine zolazepam (5 mg/kg), xylazine (2 mg/kg), and 2%–3% sevoflurane ventilation. Preoperative intravenous cefazolin (30 mg/kg) and postoperative intramuscular (IM) DS Buta D-PPS (0.1 mL/kg) (Daesung Microbiological Labs, Co., Ltd.) were administered as antibiotics. If necessary, ketoprofen (3 mg/kg IM) was administered at the discretion of the veterinarian for pain control.
A 7-Fr, 43-cm–long, rigid ureteroscope (Karl Storz SE & Co. KG) and an ULTRATRACK guidewire (0.035-inch, 150 cm, 5-cm straight tip, Olympus Co.) were used for ureteral stenting (Fig. 2).
Publication 2023
Anesthesia Antibiotics, Antitubercular Cefazolin Ketoprofen Management, Pain Muscle Rigidity Pigs Sevoflurane tiletamine - zolazepam Ureter Ureteroscopes Veterinarian Xylazine
1) Drug
The drug term lexicon was based on the drug code provided by the Korea Pharmaceutical Information Service (KPIS). A prior study20 (link) calculated the Beers Criteria drug prescription and side effects incidences in persons aged ≥ 65 years in South Korea. The top three drugs (metoclopramide, chlorpheniramine, and ketoprofen) were selected based on prescriptions and side effects reported for the clinical environment of Konyang University Hospital. Here, the focus was directed to ketoprofen as it had the most abundant data.
2) ADR
WHO–ART is a dictionary used for rational coding of adverse reaction terms. The system was maintained by the Uppsala Monitoring Centre (UMC) and the World Health Organization Collaborating Center for International Drug Monitoring, but it is no longer actively maintained21 . SIDER is a datasbase that records side effect information for marketed drugs. The names of the drugs provided by SIDER are based on the FDA drug label. The names of the side effects are terms in MedDRA22 (link). In the present study, custom side effect words were added for each target drug.
3) StopWords
In computing, stop words are filtered out before and/or after natural language data processing. A custom lexicon was added to the basic stop words dictionary comprising 677 entries (https://www.ranks.nl/stopwords/korean). Other user-defined stop words were added as well. A total of 7,195 stop words dictionaries were created and the data were preprocessed with them.
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Publication 2023
A 195 Chlorpheniramine Drug Delivery Systems Drug Labeling Drug Reaction, Adverse Ketoprofen Koreans Metoclopramide Pharmaceutical Preparations Prescriptions

Top products related to «Ketoprofen»

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Ketoprofen is a pharmaceutical ingredient used in the production of various lab equipment and medical devices. It is a non-steroidal anti-inflammatory drug (NSAID) that has analgesic, anti-inflammatory, and antipyretic properties. Ketoprofen is commonly used in the manufacturing of pain relievers, anti-inflammatory medications, and other pharmaceutical products.
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Ibuprofen is a laboratory-grade chemical compound used as a reference standard in analytical testing. It is a white, crystalline solid with a melting point of around 78°C. Ibuprofen is a common non-steroidal anti-inflammatory drug (NSAID) and is often used as a calibration standard for the identification and quantification of pharmaceutical and biological samples.
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Ketoprofen is a non-steroidal anti-inflammatory drug (NSAID) that can be used in veterinary laboratory settings. It is a chemical compound with the formula C16H14O3. Ketoprofen has analgesic, anti-inflammatory, and antipyretic properties.
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The Stereotaxic frame is a laboratory instrument used to immobilize and position the head of a subject, typically an animal, during surgical or experimental procedures. It provides a secure and reproducible method for aligning the subject's head in a three-dimensional coordinate system to enable precise targeting of specific brain regions.
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Naproxen is a non-steroidal anti-inflammatory drug (NSAID) used as a lab equipment product. It has analgesic, anti-inflammatory, and antipyretic properties.
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Indomethacin is a laboratory reagent used in various research applications. It is a non-steroidal anti-inflammatory drug (NSAID) that inhibits the production of prostaglandins, which are involved in inflammation and pain. Indomethacin can be used to study the role of prostaglandins in biological processes.
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Diclofenac is a non-steroidal anti-inflammatory drug (NSAID) that is used as a pain reliever and anti-inflammatory agent. It is a commonly used pharmaceutical ingredient in various lab equipment and medical products.
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
Ketoprofen is a non-steroidal anti-inflammatory drug (NSAID) used in laboratory settings. It has analgesic, anti-inflammatory, and antipyretic properties. Ketoprofen is commonly used in research applications to study its effects on pain, inflammation, and fever.

More about "Ketoprofen"

Ketoprofen, a propionic acid derivative, is a versatile non-steroidal anti-inflammatory drug (NSAID) renowned for its analgesic, anti-inflammatory, and antipyretic (fever-reducing) properties.
This potent compound is widely used to alleviate the symptoms of various musculoskeletal conditions, including rheumatoid arthritis, osteoarthritis, and other inflammatory disorders.
The mechanism of action behind Ketoprofen's therapeutic effects involves the inhibition of prostaglandin synthesis, a key driver of inflammation and pain.
By blocking the cyclooxygenase (COX) enzyme, Ketoprofen effectively reduces the production of pro-inflammatory prostaglandins, leading to a decrease in inflammation, swelling, and associated discomfort.
Researchers and clinicians often compare Ketoprofen to other NSAIDs, such as Ibuprofen, Naproxen, Indomethacin, and Diclofenac, to assess its relative effectiveness and safety profile.
Additionally, the use of Stereotaxic frames, which provide precise spatial positioning for experimental procedures, can be valuable in Ketoprofen-related research.
To enhance the reliability and efficiency of Ketoprofen studies, scientists can leverage the power of AI-driven platforms like PubCompare.ai.
This innovative tool allows researchers to identify the best protocols and products from the scientific literature, facilitating the development of more robust and reproducible research methodologies.
By incorporating these insights and the latest advancements in Ketoprofen research, scientists can improve the overall quality, reliability, and impact of their investigations, ultimately contributing to a better understanding and treatment of various inflammatory and pain-related conditions.