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Laminaran

Laminaran is a sulfated polysaccharide derived from brown seaweed.
It has been studied for its potential therapeutic applications, including anti-inflammatory, anti-oxidant, and anti-tumor properties.
Laminaran has also been investigated for its ability to modulate immune function and enhance wound healing.
Researchers utilze PubCompare.ai's AI-driven comparisons to identify the best laminaran-based protocols and products for their scientific investigations, enabling seamless reproducibility and accelerating their discoveries.

Most cited protocols related to «Laminaran»

1
Antioxidant Capacity of Lotus Oligomers
Cited 1 time
The DPPH radical scavenging activity of LOs was investigated by a previous method with slight modification (19 (link)). In brief, 100 μL different concentrations (0.1–2.0 mg/mL) of LOs were added to 100 μL of 50 mmol/L DPPH in ethanol. Then the mixture was shaken and incubated in dark place for 30 min, and the value of absorbance was measured at 517 nm. Ascorbic acid was used as a positive control. The ability of DPPH scavenging was calculated using the following equation:
Where A0 was the absorbance of blank (ethanol instead of sample), Ai was the absorbance of sample (sample with DPPH-methanol solution), Aj was the absorbance of control (sample without DPPH-methanol solution).
The potential of LOs in scavenging ABTS radicals was evaluated as described earlier with some adjustments (20 (link)). Briefly, ABTS radical solution was prepared as follows: 7 mmol/L of ABTS solution (5 mL) was incubated with 2.45 mmol/L of potassium persulfate (88 μL) at room temperature for 16 h. Next, 2 mL ABTS radical solution were added into LOs samples (1 mL) with various concentrations (0.0625, 0.125, 0.25, 0.5, 1, and 2 mg/mL), respectively. After rection at room temperature (in dark place) for 10 min, the value of absorbance was measured at 734 nm. Ascorbic acid was used as the positive control. The ABTS radical scavenging activity (%) was calculated as DPPH radical scavenging activity equation above.
Cheong K.L., Li J.K, & Zhong S. (2022). Preparation and Structure Characterization of High-Value Laminaria digitata Oligosaccharides. Frontiers in Nutrition, 9, 945804.
Publication 2022
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid 2-(4'-diethylaminophenyl)benzothiazole Ascorbic Acid Ethanol Methanol potassium persulfate
2
Extraction and Hydrolysis of Laminaran from Saccharina latissima
Cited 1 time
Frozen, milled S. latissima (two batches, harvested October 2014 and 2016, respectively, from the Trondheim Fjord, Norway) was thawed at 4°C over night. The extracts (SWEs) were prepared according to the protocol developed by Horn et al. (2000a) (link), but using pH ∼3.5, since later works have shown that the laminaran becomes less available for enzymatic hydrolysis after extraction at pH < 3 (Adams et al., 2008 (link); Sandbakken et al., 2018 (link)). Briefly, hot water (60–70°C) was added to the biomass (biomass:water, 1:1, weight basis), and pH was adjusted to ∼3.5 by addition of 1M H2SO4, before incubation at 70°C for 1 h to completely dissolve laminaran. The slurry was centrifuged when still hot (>60°C) (7000 rpm, 12220 × G, 15 min). The supernatant was sieved, and the pH was adjusted to pH 5.0 for enzymatic hydrolysis of laminaran or to pH 6.5–7 for cultivations without any laminaran hydrolysis.
For laminaran hydrolysis, 575–885 μl of the enzyme Cellic CTect2 (SAE0020 Sigma) was added per 100 ml SWE, followed by incubation with rotation at 50°C for 16–18 h. After incubation, the pH of the enzyme-treated extracts was adjusted to 6.5–7. The resulting extracts (SWE2) were frozen at −20°C, and either autoclaved or sterile filtered (0.22 μm) before use in shake flask and microwell cultivations.
Extract without added enzyme was treated in parallel and used as control in growth experiments. The conductivities and osmolarities of the extracts were determined using a Sension + MM 374 Laboratory meter (Hach, Loveland, CO, United States) and an Osmomat 030-D (Gonotec GmbH, Berlin, Germany), respectively.
Hakvåg S., Nærdal I., Heggeset T.M., Kristiansen K.A., Aasen I.M, & Brautaset T. (2020). Production of Value-Added Chemicals by Bacillus methanolicus Strains Cultivated on Mannitol and Extracts of Seaweed Saccharina latissima at 50°C. Frontiers in Microbiology, 11, 680.
Publication 2020
Electric Conductivity Enzymes Fjord Freezing Horns Hydrolysis laminaran Osmolarity Strains Tremor
3
Polysaccharide Characterization Protocol
Cited 1 time
Fucoidan (F5631), alginate (A7003), laminaran (L9634), carrageenan (C1013), sulfated Dextran (D6001), chondroitin (C4384), oat spelt xylan (X0627), gum Arabic (G9752) and citrus pectin (P9135) were obtained from Sigma-Aldrich. Tamarind xyloglucan, potato galactan, guar galactomannan, sugar beet arabinan and citrus polygalacturonan were obtained from Megazyme International (Bray, Ireland).
Torode T.A., Marcus S.E., Jam M., Tonon T., Blackburn R.S., Hervé C, & Knox J.P. (2015). Monoclonal Antibodies Directed to Fucoidan Preparations from Brown Algae. PLoS ONE, 10(2), e0118366.
Publication 2015
Alginate araban Beta vulgaris Carrageenan Chondroitin Citrus citrus pectin Cyamopsis Dextran fucoidan Galactans galactomannan Gum Arabic laminaran Potato Tamarindus indica Triticum spelta Xylans xyloglucan
4
Sulfated Laminaran Extraction and Modification
Native laminaran AaL from A. angusta was modified by chlorosulfonic acid/pyridine method to obtain the sulfated laminaran AaLs as described earlier [14 (link)].
Malyarenko O.S., Malyarenko T.V., Usoltseva R.V., Surits V.V., Kicha A.A., Ivanchina N.V, & Ermakova S.P. (2021). Combined Anticancer Effect of Sulfated Laminaran from the Brown Alga Alaria angusta and Polyhydroxysteroid Glycosides from the Starfish Protoreaster lincki on 3D Colorectal Carcinoma HCT 116 Cell Line. Marine Drugs, 19(10), 540.
Publication 2021
chlorosulfonic acid laminaran pyridine
5
Isolation of Laminaran from A. angusta
The laminaran AaL was isolated from the brown alga A. angusta by the methods as described previously [23 (link)].
Malyarenko O.S., Malyarenko T.V., Usoltseva R.V., Surits V.V., Kicha A.A., Ivanchina N.V, & Ermakova S.P. (2021). Combined Anticancer Effect of Sulfated Laminaran from the Brown Alga Alaria angusta and Polyhydroxysteroid Glycosides from the Starfish Protoreaster lincki on 3D Colorectal Carcinoma HCT 116 Cell Line. Marine Drugs, 19(10), 540.
Publication 2021
laminaran

Most recents protocols related to «Laminaran»

1
Laminaria digitata Phytoconstituent Preparation
The ligands used in this study were seven phytoconstituents from Laminaria digitata, including Eckol; Fucodiphlorethol G; 7-Phloroecol; dieckol, Laminaran; Alginic Acid; and Fucoidan. Each structure was downloaded from the PubChem database https://pubchem.ncbi.nlm.nih.gov/ in 3D SDF form and converted to PDB form using the Chimera software. Then, each ligand was prepared (water or unnecessary compounds were removed and hydrogen atoms were added) using AutoDock Tools and saved in the PDBQT file.
Adella Putri A.D., Sembiring M.H, & Tuba S. (2024). Phytochemical constituents analysis in laminaria digitata for Alzheimer’s disease: molecular docking and in-silico toxicity approach. Communicative & Integrative Biology, 17(1), 2357346.
Publication 2024
2
ESR Analysis of Laminaran's ROS Scavenging
The ROS-scavenging ability of laminaran was measured via ESR. Laminaran was dissolved in Milli-Q water at a concentration of 500 mg mL−1. ROS levels were measured according to the method of Oowada et al. [19 (link)]; 1O2 was generated in combination with acid red and light. This reaction mixture comprised Milli-Q water with 200 µM acid red and 10 mM TEMPOL with or without 50 mg mL−1 laminaran. •OH was generated in combination with H2O2 and light. This reaction mixture comprised Milli-Q water with 10 mM H2O2 and 10 mM CYPMPO with or without 50 mg mL−1 laminaran. O2•− was generated from a xanthine/xanthine oxidase reaction. The reaction mixture comprised Milli-Q water with 20 mM hypoxanthine, 20 units mL−1 xanthine oxidase, and 10 mM CYPMPO with or without 50 mg mL−1 laminaran. The ESR spectra of the laminaran-containing mixtures were recorded using a JEOL-TE X-band spectrometer (JEOL, Tokyo, Japan) and compared with those of each mixture without laminaran. ESR spectra were obtained under the following conditions: 20 mW incident microwave power, 9.2 GHz frequency, 0.2 mT modulation width, 7.5 mT sweep width, 0.1 s time contrast, and 335.5 mT center field. All experiments were repeated three times.
Kurokawa H., Marella T.K., Matsui H., Kuroki Y, & Watanabe M.M. (2023). Therapeutic Potential of Seaweed-Derived Laminaran: Attenuation of Clinical Drug Cytotoxicity and Reactive Oxygen Species Scavenging. Antioxidants, 12(7), 1328.
Publication 2023
Hypoxanthine laminaran Light Microwaves Peroxide, Hydrogen tempol Xanthine Oxidase
3
Laminaran Elicitation of Tomato Seedlings
Solanum lycopersicum L. seeds (cv. St Pierre) were sterilized with ethanol and sodium hypochlorite and cultivated on agar ½ MS medium at 24 °C (80–90% relative humidity (RH), 16 h to 8 h day and night cycle), in a growth chamber for 13 days. The seedlings were elicited with water, 1% (w/v) standard laminaran from L. digitata (LAM std) or 1% (w/v) laminaran LAM2 (from L. hyperborea) three days after sprouting. Root and aerial parts lengths were measured using the ImageJ software (1.54f version).
The standard laminaran (LAM std) was obtained from Sigma-Aldrich (L9634; Saint-Quentin-Fallavier, France). The laminaran LAM2 fraction was isolated using an industrial targeted Laminaria hyperborea extraction process involving ethanol precipitation. Precipitated laminaran was resuspended in water and recovered from fucoidans using an ultrafiltration step.
Mirande-Ney C., Arnaudin Q., Durambur G., Plasson C., Bernard S., Chamot C., Grivotte J., Mati-Baouche N., Driouich A., Brebion J., Hennequart F., Lerouge P, & Boulogne I. (2023). LAM2: An Unusual Laminaran Structure for a Novel Plant Elicitor Candidate. Biomolecules, 13(10), 1483.
Publication 2023
Agar Ethanol Humidity laminaran Laminaria Lycopersicon esculentum Plant Embryos Plant Roots Seedlings Sodium Hypochlorite Ultrafiltration
4
Evaluating Cell Viability with Laminaran
Cell viability was evaluated using the Cell Counting Kit-8 (CCK8) (Dojindo, Tokyo, Japan). The cells were cultured in 96-well plates at a density of 5 × 103 cells well−1 and incubated for 2 d. The supernatant was aspirated, and the cells were cultured in a medium containing 0, 1, 10, and 100 µg mL−1 laminaran with 300 µM Ind or 15 µM Dab for 24 h. After cultivation, the cells were incubated within 10% CCK8. As CCK-8Absorbance at 450 nm was measured using a Synergy H1 microplate reader (BioTek Instruments Inc., Winooski, VT, USA).
Kurokawa H., Marella T.K., Matsui H., Kuroki Y, & Watanabe M.M. (2023). Therapeutic Potential of Seaweed-Derived Laminaran: Attenuation of Clinical Drug Cytotoxicity and Reactive Oxygen Species Scavenging. Antioxidants, 12(7), 1328.
Publication 2023
Cells Cell Survival Culture Media laminaran
5
Seaweed-Derived Laminaran Antioxidant Assay
Laminaran, extracted from seaweed Eisenia bicyclis, was purchased from Tokyo Chemical Industry (CAS No.: 9008-22-4, Tokyo, Japan). Ind was purchased from Wako Pure Chemical Industries (Osaka, Japan) and dissolved in dimethyl sulfoxide (DMSO) at a concentration of 100 mM and prepared for each experiment. Dab was purchased from Cayman Chemical Co. (Ann Arbor, MI, USA) and dissolved in DMSO at a concentration of 10 mM and stored at −20 °C until use. Acid red and 1-oxyl-2,2,6,6-tetramethyl-4-hydroxypiperidine (TEMPOL) were purchased from Tokyo Chemical Industry (Tokyo, Japan). Hydrogen peroxide (H2O2) and xanthine were purchased from Wako Pure Chemical Industries. 5-(2,2-dimethyl-1,3-propoxycyclophosphoryl)-5-methyl-1-pyrroline N-oxide (CYPMPO) was purchased from Radical Research Inc. (Tokyo, Japan). xanthine oxidase was purchased from Nacalai Tesque (Kyoto, Japan).
Kurokawa H., Marella T.K., Matsui H., Kuroki Y, & Watanabe M.M. (2023). Therapeutic Potential of Seaweed-Derived Laminaran: Attenuation of Clinical Drug Cytotoxicity and Reactive Oxygen Species Scavenging. Antioxidants, 12(7), 1328.
Publication 2023
Caimans laminaran Oxides Peroxide, Hydrogen pyrroline Sulfoxide, Dimethyl tempol Xanthine Xanthine Oxidase

Top products related to «Laminaran»

1
Sugar beet arabinan by Megazyme
Sourced in Ireland
Sugar beet arabinan is a type of plant-derived polysaccharide. It is extracted from the cell walls of sugar beet. Sugar beet arabinan is a complex carbohydrate composed of arabinose units.
2
Guar galactomannan by Megazyme
Sourced in Ireland
Guar galactomannan is a polysaccharide extracted from the seeds of the guar plant (Cyamopsis tetragonoloba). It is a water-soluble fiber with a linear backbone of (1→4)-linked β-D-mannopyranosyl units with single-unit (1→6)-linked α-D-galactopyranosyl branches.
3
Tamarind xyloglucan by Megazyme
Sourced in Ireland
Tamarind xyloglucan is a polysaccharide derived from the seeds of the tamarind tree. It is a complex carbohydrate composed of a backbone of glucose units with xylose side chains. Tamarind xyloglucan is commonly used in various laboratory applications due to its unique physical and chemical properties.
4
Powerwave xs microplate reader by Agilent Technologies
Sourced in United States, Spain
The PowerWave XS microplate reader is a laboratory instrument designed for absorbance-based assays. It can measure the optical density of samples in microplates, which is a common technique used in various scientific applications.
5
Laminaran by Tokyo Chemical Industry
Sourced in Japan
Laminaran is a laboratory equipment product manufactured by Tokyo Chemical Industry. It is a polysaccharide extracted from brown algae that can be used for various applications in research and analysis. The core function of Laminaran is to serve as a biochemical reagent for experimental and analytical purposes.
6
Potato galactan by Megazyme
Sourced in Ireland
Potato galactan is a high molecular weight polysaccharide extracted from potato. It can be used for analysis and research purposes in laboratory settings.
7
L9634 by Merck Group
Sourced in France
The L9634 is a piece of laboratory equipment manufactured by Merck Group. It is designed for general laboratory use. The core function of the L9634 is to assist in the processing and analysis of samples within a controlled laboratory environment. Further details on the specific capabilities and intended use of this product are not available.
8
Synergy h1 by Agilent Technologies
Sourced in United States, Germany, Canada, China, Switzerland, Japan, United Kingdom, France, Australia
The Synergy H1 is a multi-mode microplate reader designed for a variety of applications. It is capable of absorbance, fluorescence, and luminescence detection.
9
3 3 diaminobenzidine (dab) by Cayman Chemical
Sourced in United States
DAB is a chromogenic substrate used for immunohistochemical detection of target proteins in biological samples. It produces a brown precipitate upon enzymatic conversion, allowing visualization of the labeled antigen.
10
Tlc silica gel 60f plates by Merck Group
Sourced in Germany
TLC Silica gel 60F plates are a type of thin-layer chromatography (TLC) plates used for the separation and analysis of chemical compounds. The plates are coated with a layer of silica gel, which serves as the stationary phase for the chromatographic process. The 'F' in the product name indicates the presence of a fluorescent indicator, which allows for the visualization of separated compounds under ultraviolet light.

More about "Laminaran"

Laminaran, a sulfated polysaccharide derived from brown seaweed, has been extensively studied for its potential therapeutic applications.
This unique compound has demonstrated promising anti-inflammatory, anti-oxidant, and anti-tumor properties, making it a subject of great interest in the scientific community.
One of the key advantages of laminaran is its ability to modulate immune function and enhance wound healing.
Researchers have utilized advanced AI-driven comparisons, such as those provided by PubCompare.ai, to identify the best laminaran-based protocols and products for their scientific investigations.
This approach has enabled seamless reproducibility and accelerated their discoveries.
Beyond laminaran, other polysaccharides like Sugar beet arabinan, Guar galactomannan, and Tamarind xyloglucan have also been studied for their potential therapeutic applications.
These compounds have been investigated using various analytical techniques, including the PowerWave XS microplate reader and TLC Silica gel 60F plates.
The optimization of laminaran and related polysaccharides has become a hot topic in the field of biomedical research.
Researchers are utilizing advanced platforms like PubCompare.ai to streamline their workflows and identify the most effective protocols and products for their studies.
This collaborative approach has led to breakthroughs in understanding the complex mechanisms underlying the therapeutic potential of these natural compounds.
As the scientific community continues to unravel the mysteries of laminaran and its counterparts, the future holds great promise for the development of innovative therapies and the advancement of our understanding of the natural world.
With the aid of cutting-edge technologies and collaborative research platforms, the possibilities for discovery are truly limitless.