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Lanolin

Lanolin: A Versatile Lipid Derived from Sheep Wool Grease.
Lanolin is a complex mixture of esters, fatty alcohols, and other lipids extracted from the sebum of sheep wool.
This naturally-occuring substance has a long history of use in a variety of cosmetic, pharmaceutical, and industrial applications due to its emollient, moisturizing, and protective properties.
Lanolin is commonly used in skincare products, ointments, and balms to help maintain healthy skin barrier function.
Reserach has also explored the potential of lanolin-based formulations for wound healing, drug delivery, and other medical applications.
Discover the power of this versatile lipid with PubCompare.ai, your AI-driven platform for optimizing Lanolin-based research protocols and products.

Most cited protocols related to «Lanolin»

The in-vitro skin permeation of RT from the NEG systems was evaluated using a static Franz diffusion cell [24 (link)]. A Franz diffusion cell is divided into two compartments (donor and receptor compartments) and a sample of shaved, excised dorsal skin from Wistar rats was mounted between these two compartments. The RT-NEG sample was applied to the donor compartment, while the receptor compartment was filled with release medium (PBS, pH 5.5) and the whole assembly was maintained at 37 °C. Aliquots were collected at different time intervals (0, 0.25, 0.5, 1, 2, 3, 4, 6, 8, 10, 12, and 24 h) and replaced by an equal volume of receptor media. The aliquots were analyzed using a UV spectrophotometer (λmax 325 nm) to elucidate the cumulative amount of drug that had permeated the skin by the various time intervals.
In-vitro drug deposition within the skin was evaluated using the same skin samples utilizing the tape-stripping technique [25 (link)]. The skin samples were unclipped from the Franz diffusion cells after 24 h of the permeation study and then washed with PBS. Cellophane tape was used for the tape stripping of skin. The first strip of tape was discarded due to the fact they potentially contained drug that was adhered to the surface of the skin sample. Approximately 10 strips were used in the removal of the entire subcutaneous (SC) layer of skin, in a manner that utilized the maximum area of tape. The treated skin samples and tape used for the stripping procedure were both then chopped and incubated in ethanol to completely extract the RT. Afterward, samples were sonicated for 15 min and then centrifuged at 3000 rpm for 15 min. The extracted samples were analyzed by UV spectroscopy at λmax 325 nm to measure the amount of RT deposited in the skin. This procedure for quantifying the skin permeation was then repeated for the RT-gel (accurately weighed amounts of RT dissolved in the small quantity of propylene glycol and dispersed into placebo gel to obtain RT-gel of strength 1% w/w) and RT creams (accurately weighed amounts of RT in the required quantity of cream base [composed of PEG 4000, PEG 400, lanolin, glyceryl monostearate, and poloxamer 188] to obtain a RT cream at a concentration of 1% w/w) in order to compare the skin permeation of RT with that of the developed NEG systems.
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Publication 2020
A-A-1 antibiotic Cellophane Cells Diffusion Ethanol glyceryl monostearate Lanolin Pharmaceutical Preparations Placebos Poloxamer 188 polyethylene glycol 400 Propylene Glycol Rats, Wistar Skin Skin Tape Spectrum Analysis Tissue Donors
A topical dose of 62.5 mg IMQ cream (5% Aldara; 3 M Pharmaceuticals) was applied daily to the shaved back region of mice. Control (CTL) animals were treated similarly with a non-toxic lanolin-derived occlusion cream (VWR, catalog number 56614-414). Mice in the CTL group did not exhibit macroscopic indications of skin irritation or inflammation (e.g., erythema, scaling, or induration). IMQ and CTL treatments were carried out for 5 consecutive days and mice were sacrificed on day 6 following injection with euthanasia solution (ketamine, 16.5 mg/mL; xylazine, 1.65 mg/mL) followed by cervical dislocation. Mice were weighed daily and provided saline (i.p.) as needed to supplement fluid loss associated with IMQ treatment, a well-known side effect [25 (link)]. This limited weight loss in most strains, although we note that one of five MOLF males and one of five MOLF females died prior to day 6. Samples from these mice were excluded from analyses, but for all other mice back skin and spleens were collected immediately upon sacrifice on day 6, flash frozen, and stored at −80 °C prior to isolation of total RNA. Skin adjacent to that collected for RNA was placed into formalin and processed for histology. For all strains, tissues were collected at approximately the same time of day to limit gene expression variation associated with circadian rhythms [26 (link)].
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Publication 2017
Aldara Animals Circadian Rhythms Dental Occlusion Dietary Supplements Erythema Euthanasia Females Formalin Freezing Genetic Diversity Inflammation isolation Joint Dislocations Ketamine Lanolin Males Mice, House Neck Pharmaceutical Preparations Saline Solution Skin Strains Tissues Xylazine
For live cell fluorescence microscopy studies, C. difficile strains were harvested in PBS, pelleted, and resuspended in PBS. For initial characterization of mutant phenotypes, cells were resuspended in PBS containing 1 μg/mL FM4-64 (Molecular Probes) and 15 μg/mL Hoechst 33342 (Molecular Probes). All live bacterial suspensions (4 μL) were added to a freshly prepared 1% agarose pad on a microscope slide, covered with a 22 x 22 mm #1 coverslip and sealed with VALAB (1:1:1 of vaseline, lanolin, and beeswax) as previously described [27 (link)].
DIC and fluorescence microscopy was performed using a Nikon PlanApo Vc 100x oil immersion objective (1.4 NA) on a Nikon Eclipse Ti2000 epifluorescence microscope. Multiple fields for each sample were acquired with an EXi Blue Mono camera (QImaging) with a hardware gain setting of 1.0 and driven by NIS-Elements software (Nikon). Images were subsequently imported into Adobe Photoshop CS6 for minimal adjustments in brightness/contrast levels and pseudocoloring.
DIC and fluorescence microscopy for cells that were processed for peptidoglycan labeling experiments were performed and processed with the same equipment as described above with the following differences: a Nikon PlanApo Vc 60x oil immersion objective (1.4 NA) was utilized, hardware gain setting 2.0, and fields were imaged with Z-spacing of 0.15 μm followed by deconvolution using AutoQuant 3x software (MediaCybernetics).
Quantification of total cells undergoing sporulation was determined by analyzing multiple fields for each strain at random. At least 50 cells were enumerated for each strain. Sporulating cells were identified as either having a polar septum with or without DNA staining in the forespore, a DIC-dark forespore with or without DNA staining in the forespore compartment, a DIC-bright forespore without DNA staining, or a free spore (no mother cell compartment).
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Publication 2015
Bacteria beeswax Cells DNA, A-Form FM 4-64 HOE 33342 Lanolin Microscopy Microscopy, Fluorescence Molecular Probes Peptidoglycan Phenotype Sepharose Spores Stem Cells Strains Submersion Vaseline
Strains used in this study are listed in Table S1. Homothallic (h90) strains able to switch mating type or 1:1 mixtures of heterothallic h+ × h cells (also called P × M cells) were used as indicated. Minimal sporulation liquid (MSL) media with or without nitrogen (MSL+N and MSL−N) liquid or agar were used to grow and mate the cells, respectively (Egel et al., 1994 (link)). All live-cell imaging was performed on cells placed on MSL−N with 2% electrophoresis-grade agarose pads, covered with a coverslip sealed with VALAP (1:1:1 Vaseline/lanolin/paraffin).
Genes were tagged at their endogenous genomic locus at their 3′ end, yielding C-terminally tagged proteins. This was achieved by PCR amplification of a fragment from a template plasmid with primers carrying 5′ extensions corresponding to the last 80 nucleotides of the ORF and the first 80 nucleotides of the 3′UTR, which was transformed and integrated in the genome by homologous recombination, as previously described (Bähler et al., 1998 (link)). For tagging of genes with sfGFP, a pFA6a-sfGFP-kanMX plasmid was used as a template. sfGFP was amplified from pMaM4 (a plasmid provided by M. Knop, University of Heidelberg, Heidelberg, Germany; containing yeast codon-optimized sfGFP), with primers osm2680 (5′-ccTTAATTAActccaagggtgaagagctatttac-3′; PacI site uppercase) and osm2681 (5′-aGGCGCGCCcttataaagctcgtccattccg-3′; AscI site uppercase), digested with AscI and Pac1 and ligated to similarly treated pSM674 (pFA6a-EGFP-kanMX6; described in Bähler et al., 1998 (link)). The sfGFP replaced EGFP, resulting in pFA6a-sfGFP-kanMX6 (pSM1538). We then used this vector as template for PCR-based targeted tagging of fus1, agn2, eng2, and exg3 (Bähler et al., 1998 (link)).
To yield Pmap3-driven fluorescent reporters, the map3 promoter region was amplified from genomic DNA with primers osm935 (5′-cccCTGCAGaagcatgcacgctgctcac-3′; PstI site uppercase) and osm936 (5′-agaGTCGACggtaaactcaacgtataag-3′; SalI site uppercase), digested with Pst1 and SalI, and ligated to similarly treated pSM242 (pRIP42:GFP; an integrative plasmid containing GFP under control of nmt41 promoter and a ura4+ selection marker), replacing the nmt41 promoter and yielding plasmid pSM793 (pRIP-Pmap3:GFP, ura4+). To generate a red reporter, the tdTomato tandem repeat was amplified from pFA6a-tdTomato-kanMX with primers osm944 (5′-aatGGATCCatggtgagcaagggcgaggaggtc-3′; BamHI site uppercase) and osm945 (5′-ttaCCCGGGcttgtacagctcgtccatgc-3′; XmaI site uppercase), digested with BamHI and XmaI, and ligated to similarly treated pSM793, yielding plasmid pSM1709 (pRIP-Pmap3:tdTomato; ura4+). Plasmids were linearized with NruI and integrated at the map3 promoter in h90 cells.
Publication 2015
Agar Cells citrate carrier Cloning Vectors Codon Electrophoresis Genes Genome Homologous Recombination Lanolin M Cells Nitrogen Nucleotides Oligonucleotide Primers Paraffin Plasmids Saccharomyces cerevisiae Sepharose Strains Tandem Repeat Sequences tdTomato Vaseline
This method was modified from the protocol by van der Fits [10 (link)]. The mouse was shaved on the dorsal skin. The imiquimod cream with a dose of 62.5 mg was topically administered on the mouse back every day for 8 consecutive days. The sham control mouse was treated similarly with the vanishing cream base (United States Pharmacopeia). The ingredients of vanishing cream vehicle were stearic acid (25%, w/w), triethanolamine (1.35%), lanolin (4%), propylene glycol (5%), methyl paraben (0.18%), propyl paraben (0.02%), and water (64.45%).
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Publication 2015
Imiquimod Lanolin methylparaben Mice, House Propylene Glycol propylparaben Seizures Skin stearic acid triethanolamine

Most recents protocols related to «Lanolin»

To ensure this model is useful for therapeutic evaluation, a wound healing efficacy study was performed using a formulation known to treat NM wounds in mice.20 The DDD model was then validated using a test formulation, NDH‐4338 in 49.5% PEG400 and 49.5% lanolin, that was previously shown to treat NM burns and reduce expression of associated markers such as iNOS, COX‐2 and mast cell degranulation.20 Optimized DDD model parameters were used for this study. One hour after NM application, mice were anesthetized and 20 μl of NDH‐4338 formulation or vehicle only were applied to the vesicant exposed regions. Mice were then kept unconscious for 5 min before being monitored while recovering from anesthesia. The treatment was administered every 6 h for 24 h (QID), at which point mice were euthanized and edema was evaluated.
Publication 2023
Anesthesia Burns Edema Lanolin Mast Cell Mus NOS2A protein, human polyethylene glycol 400 PTGS2 protein, human Therapeutic Uses Vesicants Wounds
Mechlorethamine hydrochloride (NM) was purchased from Sigma‐Aldrich (St. Louis, MO, USA). Methylene blue (MB) was purchased from Thermo Fisher Scientific (Fair Lawn, NJ, USA). Lanolin (C10‐30 cholesterol/lanosterol esters) and polyethylene glycol 400 were gifted from Croda Inc. (Edison, NJ, USA). NDH‐4338, a cyclooxygenase, inducible nitric oxide synthase, and acetylcholinesterase inhibitor prodrug, was synthesized as described.28 Ultrapure water (Milli‐Q® Advantage A10®, Type 1 water, MilliporeSigma, Burlington, MA, USA, water) was used throughout the study.
Publication 2023
Acetylcholinesterase Inhibitors Cholesterol Esters croda Lanolin Lanosterol Mechlorethamine Hydrochloride Methylene Blue Nitric Oxide Synthase Type II polyethylene glycol 400 Prodrugs PTGS1 protein, human
PMSS1 gland colonization was assessed as previously described (8 (link)) with the following modifications. One third of the stomach was embedded in 4% agarose in 1× phosphate-buffered saline (PBS, Gibco) and cut into 200 μm thick longitudinal sections using a Leica VT1200S Vibratome. Tissue sections were then permeabilized overnight at 4°C by gently rocking in blocking buffer comprising 3% bovine serum albumin (Sigma-Aldrich), 1% saponin (Sigma-Aldrich) and 1% Triton X-100 (Sigma-Aldrich) in phosphate-buffered saline (PBS). Stomachs were incubated with 1:1,000 rabbit polyclonal anti-H. pylori PMSS1 antibody (gift of Manuel Amieva, Stanford University) and 1:2,000 GS-II 488 (conjugated lectin from Griffonia simplicifolia, Fisher) for 2 h at 4°C with gentle rocking. After three 10-minute washes in PBS, samples were incubated with 1:2,000 Alexa Fluor 647 donkey anti-rabbit IgG (Invitrogen) and 1:2,000 DAPI for 2 h at room temperature with gentle rocking. After three 10-minute washes in PBS, sections were mounted onto glass slides with imaging spacers cut from Parafilm (Bemis) in ProLong Diamond Anti-Fade Reagent (Molecular Probes) and coverslips were sealed with VaLP (1:1:1 Vaseline:Lanolin:Paraffin). Stomach sections were imaged on a Zeiss LSM 780 laser-scanning confocal microscope, or with an UltraView spinning disk microscope (PerkinElmer). Z-stacks were collected to visualize H. pylori within glands and assessed in Volocity (Quorum Technologies) to enumerate H. pylori in glands based on fluorescent voxels as previously described (8 (link)).
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Publication 2023
Alexa Fluor 647 anti-IgG Antibodies, Anti-Idiotypic DAPI Diamond Equus asinus Griffonia Helicobacter pylori Lanolin Lectin Microscopy Microscopy, Confocal Molecular Probes Paraffin Phosphates Rabbits Saline Solution Saponin Sepharose Serum Albumin, Bovine Stomach Tissues Triton X-100 Vaseline
Two 100% lanolin products specifically indicated for breastfeeding use; HPA LANOLIN (Lansinoh Laboratories Inc., VA, USA), and PURELAN (Medela AG, Baar, CH) were evaluated, along with two other lanolin ingredients; PHARMALAN PH EU-SO-(RB) (Croda, Goole, UK) and CORONA-8 SO-(RB) (Croda, Goole, UK). Three independent batches of each commercial product were tested, while the lanolin ingredients were tested in singlicate.
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Publication 2023
croda Lanolin
Lanolin (10 g) was suspended in 50 mL of an aqueous solution of KOH 10% (w/v) and heated at reflux (110 °C) for 48 h. After cooling the mixture, all its unsaponifiable components were extracted with ethyl acetate, and the organic phase was discarded. The alkaline phase was then brought to acidic pH (~1) with HCl 30% and allowed to rest for a few minutes for the precipitation of a portion of the acid components, which was then filtered. The attained solid (A, 3.5 g), was treated with 30 mL of methanol for 2 h: a precipitate was formed and collected (B, 1.2 g) and the methanolic solution was dried under vacuum. The attained waxy solid (C, 2 g) was then treated with 10 mL of cyclohexane overnight, to obtain a precipitate (D, 0.75 g) and a solution, then evaporated under reduced pressure to obtain a solid wax (E, 1 g). See Figure 1 for reference. This procedure was performed three times, in order to have three samples for each portion, for a total of n. 15 samples.
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Publication 2023
Acids Cyclohexane ethyl acetate Lanolin Methanol Pressure Vacuum

Top products related to «Lanolin»

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Lanolin is a waxy substance extracted from the wool of sheep. It is a natural emollient and lubricant with moisturizing properties. Lanolin is commonly used as a component in various personal care and pharmaceutical products.
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NIS-Elements software is a comprehensive imaging and analysis platform developed by Nikon for its advanced microscope systems. The software's core function is to provide users with a robust and user-friendly interface for capturing, processing, and analyzing high-quality microscopic images and data.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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The IXon Ultra 897 is a scientific-grade electron-multiplying CCD (EM-CCD) camera designed for low-light imaging applications. It features high quantum efficiency, low readout noise, and high frame rates, making it suitable for a variety of scientific research and industrial uses.
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Propylene glycol is a clear, colorless, and odorless liquid that is commonly used as a solvent and humectant in various industries. It has a wide range of applications, including in the manufacturing of pharmaceuticals, cosmetics, and food products. Propylene glycol exhibits low toxicity and is generally recognized as safe for certain applications.
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Stearic acid is a saturated fatty acid with the chemical formula CH3(CH2)16COOH. It is a white, odorless, and waxy solid at room temperature. Stearic acid is commonly used as a laboratory reagent and has various industrial applications.
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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The DeltaVision microscope is a high-performance imaging system designed for advanced biological research. It utilizes deconvolution technology to capture high-resolution, three-dimensional images of cellular structures and dynamic processes. The core function of the DeltaVision microscope is to provide researchers with a powerful tool for detailed visualization and analysis of samples.
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The 488 and 561 nm lasers are compact and reliable light sources designed for use in various scientific and industrial applications. These lasers provide stable and coherent light output at their respective wavelengths, which can be utilized for a range of purposes, such as fluorescence excitation, flow cytometry, and microscopy. The core function of these lasers is to generate the specified wavelengths of light, which can then be integrated into larger systems or instrumentation as needed.
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Poly-L-lysine solution is a high-molecular-weight polymer of the amino acid L-lysine. It is commonly used as a cell culture substrate to enhance cell attachment and growth.

More about "Lanolin"

Lanolin, also known as wool grease or wool wax, is a complex mixture of esters, fatty alcohols, and other lipids extracted from the sebum of sheep wool.
This naturally-occurring substance has a long history of use in a variety of cosmetic, pharmaceutical, and industrial applications due to its emollient, moisturizing, and protective properties.
Lanolin is commonly used in skincare products, ointments, and balms to help maintain healthy skin barrier function.
Research has also explored the potential of lanolin-based formulations for wound healing, drug delivery, and other medical applications.
Lanolin can be used in combination with other ingredients like DMSO (Dimethyl Sulfoxide), Propylene glycol, Stearic acid, and Hydrochloric acid to enhance its effectiveness and versatility.
When paired with advanced imaging techniques like the DeltaVision microscope and 488 and 561 nm lasers, lanolin-based formulations can be studied in detail to optimize their performance.
The NIS-Elements software and IXon Ultra 897 camera can be utilized to capture high-quality images and analyze the behavior of lanolin in various biological and chemical systems.
Additionally, Poly-L-lysine solution can be used to improve the adhesion and attachment of cells or tissues to surfaces, facilitating the study of lanolin's interactions with living systems.
Discover the power of this versatile lipid with PubCompare.ai, your AI-driven platform for optimizing Lanolin-based research protocols and products.
Leverage the power of AI to identify the most effective Lanolin-based formulations and streamline your research efforts.
Expereince the future of scientific discovery with PubCompare.ai.