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Lipid Peroxides

Lipid peroxides are oxidized lipid molecules that can be formed through the breakdown of unsaturated fatty acids.
These reactive compounds can cause cellular damage and are implicated in various pathological conditions, such as atherosclerosis, cancer, and neurodegenerative diseases.
Reseachers studying lipid peroxides can leverage PubComapre.ai's AI-driven platform to optimize their research protocols, enhance reproducibility, and identify the most effective methods and products across the literature, preprints, and patents.

Most cited protocols related to «Lipid Peroxides»

1
Comprehensive Molecular Profiling of Astronaut Twins
Cited 10 times
The materials and methods are briefly summarized here; expanded materials and methods are included in the supplementary materials. Data were generated across human and microbial cells, including isolations of stool, saliva, skin, urine, blood, plasma, PBMCs, and immune cells that are CD4+, CD8+, and CD19+ enriched and lymphocyte-depleted (LD), from AutoMACS magnetic bead separation and validated by FACS (fig. S1). Molecular techniques included assessments of telomere length, telomerase activity, and chromosome aberration frequencies (qRT-PCR T:A, qRT-PCR TRAP, Telo-FISH, and dGH), WGBS, RNA-seq (polyA, riboRNA, and miRNA), mitochondrial quantification (qPCR and qRT-PCR), shotgun metagenome sequencing of fecal microbiome, targeted proteomics (LC-MS), untargeted proteomics (PECAN, MaxQuant for urine and SWATH-MS for plasma), targeted metabolomics (GC-MS), untargeted metabolomics (LC-MS), mitochondrial respiration (Seahorse XF), oxidative state measures (EPR), TCR and BCR (T cell and B cell receptor repertoire) profiling, 10 cognitive tests (motor praxis, visual object learning, fractal 2-back, abstract matching, line orientation, emotion recognition, matrix reasoning, digit symbol substitution, balloon analog risk, and psychomotor vigilance), vascular and ocular measures by ultrasound and optical coherence tomography, respectively, and a wide range of other biometrics (e.g., nutrition, height, and weight). Finally, a large set of biochemical profiles were measured pre-, in-, and postflight for both subjects: body mass, height, energy intake, vitamin levels (A, B6, B12, C, D, and E and 1-carbon metabolites), minerals (copper, ceruloplasmin, selenium, zinc, calcium, phosphorus, magnesium, and iodine), iron levels (ferritin, transferrin, transferrin receptors, Hgb, Hct, MCV, TIBC, and hepcidin), urine proteins (total, albumin, TTR, RBP, creatinine, metallothionein, 3-MH, nitrogen, and fibrinogen), bone markers (BSAP, PTH, OPG, RANKL, P1NP, sclerostin, and osteocalcin), collagen crosslinks (NTX, CTX, and DPD), oxidative stress and antioxidant capacity (8-OHdG, PGF2α, GPX, SOD, TAC, oxLDL, total lipid peroxides, heme, and glutathione), protein carbonyls (myeloperoxidase, lp-PLA2, neopterin, and beta-2 microglobulin), hormones and immune system markers (cytokines, testosterone, estradiol, DHEA/S, cortisol, IGF1, leptin, thyroid hormones, angiotensin, aldosterone, ANP, PRA, and insulin), and general urine chemistry (Na, K, and Cl ions; uric acid; cholesterol; triglyceride; HDL; LDL; phospholipids; renal stone risk; liver enzymes; hsCRP; NAD/P; and pH). Together, these data span 25 months for the flight subject twin (TW), who was compared with himself, either preflight, inflight, or postflight, and also with his twin control (HR) on Earth using generalized linear models (GLM), DESeq2, and fuzzy c-means clustering for longitudinal trends. All P values were corrected for multiple testing using a FDR of 0.05 or 0.01, and q values are reported in all tables.
Garrett-Bakelman F.E., Darshi M., Green S.J., Gur R.C., Lin L., Macias B.R., McKenna M.J., Meydan C., Mishra T., Nasrini J., Piening B.D., Rizzardi L.F., Sharma K., Siamwala J.H., Taylor L., Vitaterna M.H., Afkarian M., Afshinnekoo E., Ahadi S., Ambati A., Arya M., Bezdan D., Callahan C.M., Chen S., Choi A.M., Chlipala G.E., Contrepois K., Covington M., Crucian B.E., De Vivo I., Dinges D.F., Ebert D.J., Feinberg J.I., Gandara J.A., George K.A., Goutsias J., Grills G.S., Hargens A.R., Heer M., Hillary R.P., Hoofnagle A.N., Hook V.Y., Jenkinson G., Jiang P., Keshavarzian A., Laurie S.S., Lee-McMullen B., Lumpkins S.B., MacKay M., Maienschein-Cline M.G., Melnick A.M., Moore T.M., Nakahira K., Patel H.H., Pietrzyk R., Rao V., Saito R., Salins D.N., Schilling J.M., Sears D.D., Sheridan C.K., Stenger M.B., Tryggvadottir R., Urban A.E., Vaisar T., Van Espen B., Zhang J., Ziegler M.G., Zwart S.R., Charles J.B., Kundrot C.E., Scott G.B., Bailey S.M., Basner M., Feinberg A.P., Lee S.M., Mason C.E., Emmanuel M.i.g.n.o.t., Rana B.K., Smith S.M., Snyder M.P, & Turek F.W. (2019). The NASA Twins Study: A multidimensional analysis of a year-long human spaceflight. Science (New York, N.Y.), 364(6436), eaau8650.
Publication 2019
8-Hydroxy-2'-Deoxyguanosine Albumins Aldosterone Angiotensins Antioxidants BETA MICROGLOBULIN 2 BLOOD Blood Vessel Bones Calcium Carbon Cell Respiration Cells Ceruloplasmin Cholesterol Chromosome Aberrations Cognitive Testing Collagen Copper C Reactive Protein Creatinine Cytokine Dehydroepiandrosterone Sulfate Dinoprost Emotions Enzymes Estradiol Feces Ferritin Fibrinogen Fingers Fishes Gas Chromatography-Mass Spectrometry Glutathione Heme Hepcidin Homo sapiens Hormones Human Body Hydrocortisone IGF1 protein, human Insulin Iodine Ions Iron isolation Kidney Calculi Leptin Lipid Peroxides Liver Lymphocyte Magnesium Metagenome Metallothionein Microbiome MicroRNAs Minerals Mitochondria Neopterin Nitrogen Osteocalcin Oxidative Stress oxidized low density lipoprotein PAF 2-Acylhydrolase PAX5 protein, human Pecans Peroxidase Phospholipids Phosphorus Plasma Poly A procollagen Type I N-terminal peptide Proteins Receptors, Antigen, B-Cell RNA-Seq Saliva Seahorses Selenium Skin T-Lymphocyte Telomerase Telomere Testosterone Thyroid Hormones TNFSF11 protein, human Tomography, Optical Coherence Transferrin Transferrin Receptor Triglycerides Twins Ultrasonics Uric Acid Urine Vision Vitamins Wakefulness Zinc
2
Oxidative Stress Evaluation in Cells
Cited 8 times

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Publication 2020
2',7'-dichlorodihydrofluorescein diacetate Biological Assay Catabolism Cells Fluorescent Dyes Fluorometry Lipid Peroxidation Lipid Peroxides Lipolysis Malondialdehyde Oxidants Peroxide, Hydrogen Peroxides Proteins thiobarbituric acid Thiobarbituric Acid Reactive Substances Trichloroacetic Acid
3
Spectrophotometric Assay for GPx1 and GPx4
Cited 6 times

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Publication 2020
Biological Assay Cell Motility Assays Electrons Ethanol Glutathione Glutathione Disulfide Glutathione Reductase Lipid Peroxides NADP Peroxide, Hydrogen Phospholipid Hydroperoxide Glutathione Peroxidase Phospholipids Plasma Membrane Spectrum Analysis
4
Measuring Lipid Peroxidation by Modified TBARS Assay
Cited 5 times
A modified thiobarbituric acid-reactive species (TBARS) assay [14 (link)] was used to measure the lipid peroxide formed, using egg-yolk homogenates as lipid-rich media [15 (link)]. Malondialdehyde (MDA), a secondary product of the oxidation of polyunsaturated fatty acids, reacts with two molecules of thiobarbituric acid (TBA), yielding a pinkish red chromogen with an absorbance maximum at 532 nm [16 (link)]. Egg homogenate (250 μL, 10% in distilled water, v/v) and 50 μL of extract were mixed in a test tube and the volume was made up to 500 μL, by adding distilled water. Finally, 25 μL “FeSO4” (0.07 M) was added to the above mixture and incubated for 30 min, to induce lipid peroxidation. Thereafter, 750 μL of 20% acetic acid (pH 3.5) and 750 μL of 0.8% TBA (w/v) (prepared in 1.1% sodium dodecyl sulphate) and 25 μL 20% TCA were added, vortexed, and then heated in a boiling water bath for 60 min. After cooling, 3.0 mL of 1-butanol was added to each tube and centrifuged at 3000 rpm for 10 min. The absorbance of the organic upper layer was measured against 3 mL butanol at 532 nm. For the blank 50 μL of distilled water was used in place of the extract.
Upadhyay R., Chaurasia J.K., Tiwari K.N, & Singh K. (2014). Antioxidant Property of Aerial Parts and Root of Phyllanthus fraternus Webster, an Important Medicinal Plant. The Scientific World Journal, 2014, 692392.
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Publication 2014
Acetic Acid azo rubin S Bath Biological Assay Butyl Alcohol Lipid Peroxidation Lipid Peroxides Lipids Malondialdehyde Polyunsaturated Fatty Acids Sulfate, Sodium Dodecyl thiobarbituric acid Yolks, Egg
5
Mitochondrial Superoxide Production Measurement
Cited 5 times

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Publication 2012
Anabolism Egtazic Acid Fluorescence Glucose HEPES Hexokinase Horseradish Peroxidase Hydroxyl Radical Ketoglutarate Dehydrogenase Complex Lipid Peroxides Magnesium Chloride malate Mitochondria Oligomycins Oxidoreductase Peroxidase Peroxide, Hydrogen Proteins Pyruvate Rotenone Serum Albumin, Bovine Superoxide Dismutase Superoxides

Most recents protocols related to «Lipid Peroxides»

1
Quantifying Lipid and DNA Oxidation
Lipid peroxidation was assessed using a colorimetric assay kit, by measuring free MDA as decomposition products of lipid peroxides, and DNA peroxidation was measured using an enzyme-linked immunoassay kit that measures 8-OHdG as a marker of DNA peroxides. All assay procedures were performed according to the manufacturers' instructions. The values of the MDA and 8-OHdG were calculated as μmol/mg and ng/mL, respectively.
Kong W., Zhou W., He Z., Zhang X., Li S., Zhong R, & Liu J. (2023). Polymerized human cord hemoglobin assisted with ascorbic acid as a red blood cell substitute alleviating oxidative stress for blood transfusion. Frontiers in Bioengineering and Biotechnology, 11, 1151975.
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Publication 2023
8-Hydroxy-2'-Deoxyguanosine Biological Assay Colorimetry DNA hydroperoxide Enzyme Immunoassay Lipid Peroxidation Lipid Peroxides
2
Lipid Peroxidation Assay in Dissociated Motor Neurons
Dissociated motor neurons were seeded on a six‐well plate and incubated for 2 days. Cells were treated with RSL3 overnight. The next day, BODIPY™581/591 C11 (cat. D3861; Thermo Fisher Scientific, Waltham, MA, USA) was added to each well to the final concentration of 1 μm, and the culture plate was incubated for 20 min at 37 °C. Cells were harvested using a cell scraper and centrifuged to make a cell pellet. The pellet was resuspended and washed with Hanks' balanced salt solution (HBSS; cat. 14025092; Thermo Fisher Scientific) to remove excess BODIPY‐C11 dye. After washing, cells were pelleted again by spinning, and the cell pellet was resuspended in 500 μL of HBSS. Next, the cell suspension was strained through a 40‐μm cell strainer (BD, San Jose, CA, USA), followed by flow cytometry analysis using Guava easyCyte Plus (Millipore, Billerica, MA, USA). BODIPY‐C11 signal, which reflects the lipid peroxide level, was measured using the FL1 channel. Experiments were performed in biological triplicates, and a representative result was shown.
Martinez A.M., Kim A., Flores C.A., Rahman D.F, & Yang W.S. (2023). Mouse embryonic stem cell‐derived motor neurons are susceptible to ferroptosis. FEBS Open Bio, 13(3), 419-433.
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Publication 2023
Biopharmaceuticals BODIPY Cells Flow Cytometry Hanks Balanced Salt Solution Hemoglobin, Sickle Lipid Peroxides Motor Neurons Psidium guajava
3
Antioxidant Analysis in Mice Jejunum
According to Tsakiris et al. (40 (link)), pieces of mice jejuna from all experimental groups were weighed and homogenized to produce 10% (w/v) homogenate in an ice-cold solution containing 300 mM sucrose and 50 mM Tris(hydroxymethyl)aminomethane-HCl (Sigma, St Louis, MO, USA). The homogenate was centrifuged at 500×g at 4 °C for 10 min. The supernatant was separated and used in a variety of biochemical analyses. According to the technique used by Beutler et al. (41 ), the concentrations of reduced glutathione (GSH) were calculated using the absorbance measurement at 405 nm and expressed as mg/g. The level of glutathione peroxidase (GPx) was assessed using the Pagila and Valentine (42 ) method, which reported values as U/g. Lipid peroxide by-product as malondialdehyde (MDA) contents (43 (link)) were estimated in the supernatant of jejunum homogenate at 534 nm and represented as nmol/g. The Montgomery and Dymock (44 ) procedure were used to measure the level of nitric oxide (NO), which was then represented as µmol/L. The quantity of superoxide dismutase (SOD) was quantified at 560 nm and displayed as U/g (45 (link)). All these parameters were measured using the proper kits (Biodiagnostic Co., Egypt), which were supported by the software of SoftMax® Pro v. 6.3.1. The molecular device used for the analysis was the Spectra MAX 190.
Abdel-Gaber R., Hawsah M.A., Al-Otaibi T., Alojayri G., Al-Shaebi E.M., Mohammed O.B., Elkhadragy M.F., Al-Quraishy S, & Dkhil M.A. (2023). Biosynthesized selenium nanoparticles to rescue coccidiosis-mediated oxidative stress, apoptosis and inflammation in the jejunum of mice. Frontiers in Immunology, 14, 1139899.
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Publication 2023
Cold Temperature Jejunum Lipid Peroxides Malondialdehyde Medical Devices methylamine Mus Oxide, Nitric Peroxidase, Glutathione Reduced Glutathione Sucrose Superoxide Dismutase Tromethamine
4
Detecting ROS-Induced Membrane Disruption
ROS destroying the bacterial membrane generally accompanies with the production of lipid peroxide radical, thereby forming the malondialdehyde (MDA). To verify the role of ROS in breaking the bacterial membrane (Ayaz Ahmed and Anbazhagan, 2017 (link)), MDA expression was detected by TBA assay. Briefly, 10% TCA was introduced into the bacterial liquid that was co-cultured with EG for 24 h, followed by the addition of 0.67% TBA to incubate for 1 h at 95°C. After being cooled to room temperature, the reaction mixture was centrifuged at 6000 rpm for 15 min, and the absorbance of the supernatant was measured at 532 nm using a microplate reader. Bacteria treated with 10 μM hydrogen peroxide and untreated bacteria were used as positive and negative controls, respectively.
In addition, excessive ROS is inclined to reduce the intracellular concentration of GSH and then weaken the anti-oxidation ability of bacteria, thus leading to lipid peroxidation of the membrane (Rahman et al., 2007 (link)). Hence, GSH expression induced by EG was detected by DTNB assay. Briefly, the bacterial liquid, co-cultured with EG for 24 h, was collected and cracked on ice with 10% TCA solution for 15 min. Then, 200 μl of the bacterial lysate was mixed with 1,800 μl Tris buffer (30 mM, pH 8.3) and 100 μl 0.1% DTNB for the incubation of 90 min in the dark at room temperature. After that, the absorbance of solutions was monitored at 412 nm using a microplate reader. Bacteria treated with 10 μM hydrogen peroxide and untreated bacteria were used as positive and negative controls, respectively. The relative production of MDA or GSH was estimated as the following formula:
Where ODs, ODn, and ODp represent the detected absorbance of solutions for the sample group, negative and positive control, respectively.
Liu W., Chen G., Dou K., Yi B., Wang D., Zhou Q, & Sun Y. (2023). Eugenol eliminates carbapenem-resistant Klebsiella pneumoniae via reactive oxygen species mechanism. Frontiers in Microbiology, 14, 1090787.
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Publication 2023
Bacteria Biological Assay Dithionitrobenzoic Acid Lipid Peroxides Malondialdehyde Membrane Lipids Peroxide, Hydrogen polyvalent mechanical bacterial lysate Protoplasm Tissue, Membrane Tromethamine
5
Oxygen Radical Absorbance Capacity Assay
An oxygen radical absorbance capacity (ORAC) assay was conducted per the method in the literature65 (link). Briefly, 100 μL of fluorescein (10 μM) and a 50 μL sample (0.1 mg/mL) of Trolox (15–250 μM) were transferred into a 96-well plate. Subsequently, 50 μL of 2, 2’-azobis(2-methylpropionamidine) dihydrochloride (500 mM) was added and the fluorescence of the mixture was recorded for 2 h at 485-nm excitation and 528-nm emission in a Synergy LX microplate reader (BioTek, Winooski, VT, USA). The ORAC was reported in terms of micromoles of Trolox equivalents (TE) per gram of dry RPH sample (µmol TE/g RPH)65 (link). ROO·+AH→ROOH+A· ROO·+FLH→ROOH+FL·
Chen H.J., Dai F.J., Chen C.Y., Fan S.L., Zheng J.H., Chau C.F., Lin Y.S, & Chen C.S. (2023). Effects of molecular weight fraction on antioxidation capacity of rice protein hydrolysates. Scientific Reports, 13, 3464.
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Publication 2023
Biological Assay Fluorescein Fluorescence Lipid Peroxides Oxygen Radical Absorbance Capacity Trolox C

Top products related to «Lipid Peroxides»

1
Lipid hydroperoxide assay kit by Cayman Chemical
Sourced in United States
The Lipid Hydroperoxide Assay Kit is a quantitative colorimetric assay designed to measure lipid hydroperoxide levels in a variety of biological samples. The kit utilizes ferrous ion oxidation-xylenol orange (FOX) methodology to detect and quantify lipid hydroperoxides.
2
Liperfluo by Dojindo Laboratories
Sourced in Japan, United States, China
Liperfluo is a fluorescent dye used for the detection and quantification of lipid peroxidation in biological samples. It functions by specifically reacting with lipid peroxides to produce a fluorescent signal that can be measured using a fluorescence spectrophotometer.
3
Tbars assay kit by Cayman Chemical
Sourced in United States, United Kingdom, Estonia, Japan
The TBARS assay kit is a laboratory tool used to measure the levels of thiobarbituric acid reactive substances (TBARS) in biological samples. TBARS are commonly used as a biomarker for oxidative stress and lipid peroxidation. The kit provides the necessary reagents and protocols to perform this analysis.
4
Thiobarbituric acid by Merck Group
Sourced in United States, Germany, India, United Kingdom, Sao Tome and Principe, Poland, Spain, Italy, Brazil, Macao, France, China, Czechia, Portugal, Canada, Mexico, Japan
Thiobarbituric acid is a chemical compound used in various laboratory applications. It is a white to pale yellow crystalline solid that is soluble in water and organic solvents. Thiobarbituric acid is commonly used as a reagent in analytical techniques to detect the presence of certain compounds, particularly those related to lipid peroxidation.
5
Ultrospec 5000 by GE Healthcare
Sourced in United Kingdom
The Ultrospec 5000 is a high-performance UV/Visible spectrophotometer designed for accurate and reliable measurements in a wide range of laboratory applications. It features a high-resolution display, intuitive user interface, and advanced data analysis capabilities.
6
Bodipy 581 591 c11 by Thermo Fisher Scientific
Sourced in United States, Germany, China, Italy, Japan
BODIPY 581/591 C11 is a fluorescent lipid probe used for the detection and quantification of lipid peroxidation in biological samples. It exhibits a shift in fluorescence emission from red to green upon oxidation, allowing for the monitoring of oxidative processes.
7
Dc protein assay kit by Bio-Rad
Sourced in United States, United Kingdom, Germany, Italy, Australia, Spain, China, Japan, France, Canada, Belgium, Czechia, Sweden
The DC protein assay kit is a colorimetric-based protein quantification method developed by Bio-Rad. It allows for the determination of protein concentration in aqueous solutions.
8
Image it lipid peroxidation kit by Thermo Fisher Scientific
Sourced in United States, United Kingdom
The Image-iT™ Lipid Peroxidation Kit is a fluorescence-based assay designed to detect and quantify lipid peroxidation in biological samples. The kit provides the necessary reagents and protocols to measure the formation of lipid hydroperoxides, a key indicator of oxidative stress.
9
Victor x3 by PerkinElmer
Sourced in United States, Finland, Italy, Germany, France, Singapore, Netherlands, Canada
The Victor X3 is a multimode microplate reader designed for a wide range of detection technologies. It offers high-performance detection capabilities for various applications, including absorbance, fluorescence, and luminescence measurements. The Victor X3 provides reliable and reproducible results, making it a versatile tool for researchers and scientists working in various fields.
10
Hanks balanced salt solution (hbss) by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Japan, France, Canada, Switzerland, Sweden, Italy, China, Australia, Austria, Ireland, Norway, Belgium, Spain, Denmark
HBSS (Hank's Balanced Salt Solution) is a salt-based buffer solution commonly used in cell culture and biological research applications. It provides a balanced ionic environment to maintain the pH and osmotic pressure of cell cultures. The solution contains various inorganic salts, including calcium, magnesium, and potassium, as well as glucose, to support cell viability and homeostasis.

More about "Lipid Peroxides"

Lipid peroxides, also known as lipid hydroperoxides, are highly reactive compounds formed during the oxidation of unsaturated fatty acids.
These molecules can cause significant cellular damage and have been implicated in various pathological conditions, including atherosclerosis, cancer, and neurodegenerative diseases.
Researchers studying lipid peroxides can leverage advanced tools and technologies to optimize their research protocols and enhance the reproducibility of their findings.
PubCompare.ai's AI-driven platform, for example, can help scientists identify the most effective methods and products for lipid peroxide analysis across the literature, preprints, and patents.
One key technique for measuring lipid peroxides is the Lipid hydroperoxide assay kit, which utilizes the ferrous oxidation-xylenol orange (FOX) method to quantify these reactive compounds.
The Liperfluo assay is another option, using the BODIPY 581/591 C11 fluorescent probe to detect lipid peroxidation in live cells.
The TBARS (Thiobarbituric Acid Reactive Substances) assay is a widely used method for indirectly measuring lipid peroxidation, where malondialdehyde (a lipid peroxide breakdown product) reacts with thiobarbituric acid to form a colored complex.
The Ultrospec 5000 spectrophotometer can be used to measure the absorbance of this complex.
For more targeted lipid peroxide detection, the Image-iT™ Lipid Peroxidation Kit employs the fluorescent BODIPY 581/591 C11 probe to visualize lipid peroxidation in live cells using fluorescence microscopy and the Victor X3 multimode plate reader.
Researchers may also utilize the DC protein assay kit to determine the protein content of their samples, which is essential for normalizing lipid peroxide measurements.
Additionally, Hanks' Balanced Salt Solution (HBSS) is a common buffer used in lipid peroxide studies to maintain cell viability and ensure accurate measurements.
By leveraging these advanced tools and techniques, researchers can streamline their lipid peroxide studies, enhance the reproducibility of their findings, and gain valuable insights into the role of these reactive compounds in various disease processes.