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Lipofectamine 2000

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Most cited protocols related to «Lipofectamine 2000»

1
Measuring DNA Repair by Transient Transfection
Cited 146 times
To measure the repair by transient transfection, 2.5×104 cells/cm2 were plated and transfected the next day with 0.8 µg/ml of pCBASce mixed with 3.6 µl/ml of Lipofectamine 2000 (Invitrogen) along with a variety of other vectors. The KU and RAD52 expression vectors were added at 0.8 µg/ml, the ERCC1 vector was added at 0.4 µg/ml, the RAD51-K133R vector was added at 0.1 µg/ml, and the BRC3 vector was added at 0.2 µg/ml. For each experiment, an equivalent amount of empty vector (pCAGGS-BSKX) was included in the parallel transfections. Each of these expression vectors have been previously described [18] (link). GFP positive cells were quantified by flow cytometric analysis (FACS) 3d after transfection on a Cyan ADP (Dako). Amplification of PCR products from sorted GFP+ cells, associated restriction digests, and quantification of bands were performed using the primers KNDRF and KNDRR as previously described for analysis of DR-GFP [50] (link).
To measure repair using the inducible I-SceI protein (TST) in combination with siRNA-mediated inhibition of CtIP, HEK293 cell lines with each of the reporters and stable expression of TST were first plated on 24 well plates at 105 cells/well. The following day, the wells were transfected with 70nM siRNA duplex mixed with 4ul/ml of Lipofectamine 2000 in Optimem (Invitrogen). After 4.5h, transfection complexes were diluted two-fold with media without antibiotics, and 48h after the initiation of transfection, 4OHT was added at 3 µM for 24h. Three days after 4OHT was added, the percentage of GFP+ cells was analyzed by FACS as described above. Knockdown of CtIP levels using the various siRNAs was confirmed by RT-PCR from RNA samples isolated from parallel transfections at the time of 4OHT addition (data not shown). Amplification product was quantified at the threshold cycle by including SYBR green in the PCR reaction and using an iQ5 cycler for real-time analysis at the end of each cycle (BioRad). Products were normalized relative to a primer set directed against actin. Sequences of the siRNAs siCtIP-p (Santa Cruz Biotechnology), and siCtIP-1 [25] (link), and primers for RT-PCR are shown in Figure S1D.
Repair frequencies are the mean of at least three transfections or four 4OHT treatments, and error bars represent the standard deviation from the mean. For some experiments, repair frequencies are shown relative to samples co-transfected with I-SceI and an empty vector (EV). For this calculation, the percentage of GFP+ cells from each sample was divided by the mean value of the EV samples treated in the parallel experiment. Similarly, to calculate the fold-difference in repair between siRNA-treated and control-siRNA treated cells, the percentage of GFP+ cells from each sample was divided by the mean value of control-siRNA samples from the parallel experiment. Statistical analysis was performed using the unpaired t-test.
Bennardo N., Cheng A., Huang N, & Stark J.M. (2008). Alternative-NHEJ Is a Mechanistically Distinct Pathway of Mammalian Chromosome Break Repair. PLoS Genetics, 4(6), e1000110.
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Publication 2008
Actins Antibiotics Cloning Vectors Flow Cytometry HEK293 Cells lipofectamine 2000 Oligonucleotide Primers Psychological Inhibition RAD52 protein, human RBBP8 protein, human Reverse Transcriptase Polymerase Chain Reaction RNA, Small Interfering SYBR Green I Synapsin I Transfection Transients
2
TALEN-Mediated Gene Editing Protocols
Cited 77 times
One of the pairs of TALENs targeting the human HPRT1 gene was subcloned into the mammalian expression vector pCDNA3.1(-) (Invitrogen) using XhoI and AflII. These enzymes excise the entire TALEN from pTAL3 or pTAL4 and place the coding sequence under control of the CMV (cytomegalovirus) promoter. The resulting plasmids were introduced into HEK293T cells by transfection using Lipofectamine 2000 (Invitrogen) following the manufacturer's protocol. Cells were collected 72 h after transfection and genomic DNA isolated and digested with Hpy188I, which cuts in the spacer sequence of the TALEN target site. After digestion, a chromosomal fragment encompassing the target site was amplified by PCR. Upon completion, the reactions were incubated for 20 min at 72°C with 4 µl of Taq DNA polymerase. PCR products then were digested with Hpy188I and cloned in a TOPO TA vector (Invitrogen). Independent clones containing the full-length PCR product were sequenced to evaluate mutations at the cleavage site.
The TALENs targeting the Arabidopsis ADH1 gene were subcloned into the plant expression vector pFZ14 (27 (link)) using XbaI and SacI. These enzymes excise the entire TALEN from pTAL3 or pTAL4 and place the coding sequence under control of the CaMV (cauliflower mosaic virus) 35S promoter. Recombinant plasmids were transformed into Arabidopsis protoplasts as previously described (27 (link)). Forty-eight hours after transformation, DNA was prepared and digested with PflFI, which cuts in the spacer sequence of the TALEN target site. After digestion, a chromosomal fragment encompassing the target site was amplified by PCR and the reaction products were once again digested with PflFI and run on an agarose gel. The band corresponding in size to undigested product was excised and cloned and individual clones were sequenced to evaluate mutations at the cleavage site.
Cermak T., Doyle E.L., Christian M., Wang L., Zhang Y., Schmidt C., Baller J.A., Somia N.V., Bogdanove A.J, & Voytas D.F. (2011). Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Research, 39(12), e82.
Publication 2011
Arabidopsis Cauliflower Mosaic Virus Cells Chromosomes Clone Cells Cloning Vectors Cytokinesis Cytomegalovirus Digestion DNA polymerase C Enzymes Genes Genome lipofectamine 2000 Mammals Mutation Open Reading Frames Plants Plasmids Protoplasts Sepharose Topotecan Transcription Activator-Like Effector Nucleases Transfection
3
Efficient CRISPR Editing in Cell Lines
Cited 68 times
K562 cells (American Type Culture Collection) were cultured in RPMI 1640 (Gibco) supplemented with 10% fetal bovine serum (FBS, HyClone), 1% penicillin/streptomycin. A pool of K562 cells stably expressing green fluorescent protein (GFP) was generated by transduction with the lentivirus construct pCCLsin.PPT.hPGK.GFP.pre (10 (link)). Because the lentiviral construct integrates randomly, the distribution of GFP expression levels is broad. This cell pool also includes cells that that were not transduced and do not express GFP at all. For transient transfection with CRISPR vectors, 1 × 106 K562 cells were resuspended in Nucleofector Solution V (Lonza) with 1 μg plasmid DNA, and electroporated in an Amaxa 2D Nucleofector using program T-016. In case of LBR editing, a clonal K562 line stably transformed with Cas9 was used.
Human retinal pigment epithelial (RPE) cells were cultured in a 1:1 mixture of Dulbecco's modified Eagle's medium (Gibco) with Nutrient F12 (Gibco) supplemented with 10% FBS (HyClone), 1% penicillin/streptomycin. CRISPR vectors were transfected with 5 μl Lipofectamine 2000 Reagent (Invitrogen) and 2.5 μg plasmid DNA in 250 μl antibiotic-free medium (Gibco).
Kc167 cells were cultured in Shields and Sang M3 Insect Medium (Sigma-Aldrich) with 0.25% Bacto Peptone (BD), 0.1% Yeast Extract (BD), 5% heat-inactivated FBS and 1% penicillin/streptomycin. Note that 1 × 106 cells were electroporated with 1 μg each of Cas9 and sgRNA expression plasmid using a BioRad Gene Pulser II (450 μF, 86 V).
Brinkman E.K., Chen T., Amendola M, & van Steensel B. (2014). Easy quantitative assessment of genome editing by sequence trace decomposition. Nucleic Acids Research, 42(22), e168.
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Publication 2014
5-hydroxyethoxy-N-acetyltryptamine Antibiotics Bacto-peptone Cells Clone Cells Cloning Vectors Clustered Regularly Interspaced Short Palindromic Repeats Epithelial Cells Genes Genetic Vectors Green Fluorescent Proteins Homo sapiens Insecta K562 Cells Lentivirus lipofectamine 2000 Nutrients Penicillins Plasmids Retinal Pigments Streptomycin Transients Yeast, Dried
4
Visualizing APEX-Fusion Protein Localization
Cited 67 times
Genes were introduced into HEK 293T or COS-7 cells either through transient transfection with Lipofectamine 2000 or lentiviral infection. For lipofection, 100 ng of the APEX-fusion plasmid and 0.7 μL Lipofectamine 2000 in MEM (without serum) was used per well of a 48-well plate (0.95 cm2) of cells at 60-80% confluence. 3-6 hours later, the cell culture medium was changed back to fresh growth media. After 24 hours, biotin-phenol labeling was initiated by changing the medium to 200 μL of fresh growth media containing 500 μM biotin-phenol. This was incubated at 37°C under 5% CO2 for 30 minutes according to previously published protocols 1 (link). Afterwards, 2 μL of 100 mM H2O2 was added to each well, for a final concentration of 1 mM H2O2, and the plate gently agitated for 1 minute. The reaction was then quenched by addition of 200 μL of 10 mM Trolox and 20 mM sodium ascorbate in DPBS (for a final concentration of 5 mM Trolox and 10 mM sodium ascorbate). Cells were washed with DPBS containing 5 mM Trolox and 10 mM sodium ascorbate three times and fixed with 3.7% paraformaldehyde in DPBS at room temperature for 10 min. Cells were then washed with DPBS three times and permeabilized with cold methanol at -20°C for 5 min. Cells were washed again three times with DPBS and blocked for 1 hour with 3% BSA in DPBS (“blocking buffer”) at 4°C. To detect APEX-fusion expression, cells were incubated with either mouse-α-FLAG antibody (Agilent, 1:500 dilution) or mouse-α-V5 antibody (Invitrogen, 1:500 dilution) for 1 hour to overnight at 4°C. After washing three times with 0.2% Tween in DPBS, cells were simultaneously incubated with secondary Alexa Fluor 488 goat anti-mouse IgG (Life Technologies, 1:750 dilution) and homemade streptavidin-Alexa Fluor 568 conjugates for 1 hour at 4°C. Cells were then washed three times with 0.2% Tween in DPBS and maintained in DPBS on ice for imaging.
Confocal imaging was performed using a Zeiss Axio Observer. Z1 microscope equipped with a Yokogawa spinning disk confocal head and a Cascade II: 512 camera. The confocal head contained a Quad-band notch dichroic mirror (405/488/568/647 nm). Samples were excited by solid state 491 nm (∼20 mW) or 561 nm (∼20 mW) lasers. Images were acquired using Slidebook 5.0 software (Intelligent Imaging Innovations), through a 48 × oil-immersion objective for YFP/AF488 (528/38 emission filter), AF568 (617/73 emission filter), and differential interference contrast (DIC) channels. Acquisition times ranged from 10 to 1000 milliseconds. Imaging conditions and intensity scales were matched for each dataset presented together unless otherwise noted.
Lam S.S., Martell J.D., Kamer K.J., Deerinck T.J., Ellisman M.H., Mootha V.K, & Ting A.Y. (2014). Directed evolution of APEX2 for electron microscopy and proteomics. Nature methods, 12(1), 51-54.
Publication 2014
5
High-Resolution Intracellular Ca2+ Imaging
Cited 58 times
Cultured cells were transfected using Lipofectamine 2000 (Invitrogen) 2 or 3 days before imaging. Jurkat T cells were electroporated using a MicroPorator (MP-100, Digital Bio) 1 day before imaging. For cytosolic Ca2+ imaging using fura-2, cells were loaded with 5 μM fura-2 AM (Molecular Probes, USA) at room temperature (22–24 °C) for 40–60 min in 0.1% BSA-supplemented physiological salt solution (PSS) containing (in mM) 150 NaCl, 4 KCl, 2 CaCl2, 1 MgCl2, 5.6 glucose and 25 HEPES (pH 7.4). Before imaging, the loading solution was replaced with PSS without BSA.
The images were captured using an inverted microscope (IX81, Olympus, Japan) equipped with a × 20 objective (numerical aperture (NA)=0.75, UPlanSApo, Olympus) or a × 40 objective (NA 0.90, UApo/340, Olympus), an electron-multiplying cooled-coupled device (EM-CCD) camera (ImagEM, Hamamatsu Photonics, Japan), a filter wheel (Lambda 10-3, Sutter Instrument, USA), a xenon lamp (ebx75) and a metal halide lamp (EL6000, Leica, Germany) at a rate of one frame per 2 or 3 s with the following excitation/emission filter settings: 472±15 nm/520±17.5 nm for G-GECO1.1, CEPIA1er, G-CEPIA1er, CEPIA2–4mt and EYFP-er; 562±20 nm/641±37.5 nm for R-GECO1, R-CEPIA1er and mCherry-STIM1; 377±25 nm/466±20 nm and 377±25 nm/520±17.5 nm for GEM-GECO1 and GEM-CEPIA1er; 340±13 nm/510±42 nm and 365±6 nm/510±42 nm for fura-2; 440±10.5 nm/480±15 nm and 440±10.5 nm/535±13 nm for D1ER19 (link)20 (link). For analysis of the ratiometric indicators, we calculated the fluorescence ratio (F466/F520 for GEM-GECO1 and GEM-CEPIA1er; F340/F365 for fura-2; F535/F480 for D1ER). Photobleaching was corrected for using a linear fit to the fluorescence intensity change before agonist stimulation. All images were analysed with ImageJ software.
To image subcellular ER Ca2+ dynamics during agonist-induced Ca2+ wave formation, we imaged HeLa cells expressing either G-CEPIA1er or R-CEPIA1er. Images were captured at a rate of one frame per 30–100 ms using a × 60 objective (NA 1.45, PlanApo TIRF, Olympus) and the metal halide lamp or an LED lamp (pE-100, CoolLED, UK). To evaluate Ca2+ wave velocity in the ER and cytosol, images were normalized by the resting intensity, and a linear region of interest (ROI) was defined along the direction of wave propagation. A line-scan image was created by averaging 30 adjacent linear ROIs parallel to the original ROI, and time derivative was obtained to detect the time point that showed maximal change during the scan duration. Then, the time points were plotted against the pixel, and the wave velocity was estimated by the slope of the least-squares regression line.
For mitochondrial Ca2+ imaging with ER and cytosolic Ca2+, mitochondrial inner membrane potential or mitochondrial pH at subcellular resolution, we imaged HeLa cells with a confocal microscope (TCS SP8, Leica) equipped with a × 63 objective (NA 1.40, HC PL APO, Leica) at a rate of one frame per 2 or 3 s with the following excitation/emission spectra: R-GECO1mt (552 nm/560–800nm), G-CEPIA1er (488 nm/500–550 nm) and GEM-GECO1 (405 nm/500–550 nm); GEM-GECO1mt (405 nm/500–550 nm), JC-1 (488 nm/500–550 nm and 488 nm/560–800nm); R-GECO1mt (552 nm/560–800nm), SypHer-dmito (405 nm/500–550 nm and 488 nm/500–550 nm). For analysis of JC-1 and SypHer-dmito, we calculated the fluorescence ratio (488 nm/560–800 nm over 488 nm/500–550 nm for JC-1 (ref. 55 (link)); 488 nm/500–550 nm over 405 nm/500–550 nm for SypHer-dmito62 (link)).
To perform in situ Ca2+ titration of CEPIA, we permeabilized the plasma membrane of HeLa cells with 150 μM β-escin (Nacalai Tesque, Japan) in a solution containing (in mM) 140 KCl, 10 NaCl, 1 MgCl2 and 20 HEPES (pH 7.2). After 4 min treatment with β-escin, we applied various Ca2+ concentrations in the presence of 3 μM ionomycin and 3 μM thapsigargin, and estimated the maximum and minimum fluorescent intensity (Rmax and Rmin), dynamic range (Rmax/Rmin), Kd and n.
For the estimation of [Ca2+]ER based on the ratiometric measurement using GEM-CEPIA1er (Figs 1e,f and 5b and Supplementary Fig. 5f), [Ca2+]ER was obtained by the following equation:

where R=(F at 466 nm)/(F at 510 nm), n=1.37 and Kd=558 μM.
To evaluate pH-dependent change of EYFP-er fluorescence (Supplementary Fig. 4a–d), we stimulated HeLa cells expressing EYFP-er in a PSS (adjusted to pH 6.8) containing monensin (10 μM, Wako) and nigericin (10 μM, Wako). Subsequently, the cells were alkalinized with a solution containing (in mM) 120 NaCl, 30 NH4Cl, 4 KCl, 2 CaCl2, 1 MgCl2, 5 HEPES and 5.6 Glucose (pH 7.4)67 (link).
Suzuki J., Kanemaru K., Ishii K., Ohkura M., Okubo Y, & Iino M. (2014). Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA. Nature Communications, 5, 4153.
Publication 2014
Aftercare Cells Cultured Cells Cytosol Electrons Escin Fingers Fluorescence Fura-2 fura-2-am Glucose HeLa Cells HEPES Ionomycin Jurkat Cells lipofectamine 2000 Magnesium Chloride Medical Devices Membrane Potential, Mitochondrial Metals Microscopy Microscopy, Confocal Mitochondria Molecular Probes Monensin Nigericin physiology Plasma Radionuclide Imaging Reading Frames Reproduction Sodium Chloride STIM1 protein, human Thapsigargin Titrimetry Xenon

Most recents protocols related to «Lipofectamine 2000»

1
Lipofectamine 2000 Plasmid Transfection
Plasmid transfection was conducted using Lipofectamine 2000 with cells at 70-80% confluency. Per microgram of plasmid DNA, 2.5 μl of Lipofectamine 2000 was used. After incubation, DNA-Lipofectamine 2000 complexes were added to the cells. Post-transfection, cells were incubated for 48 hours for optimal gene expression.
Du G., Jiang Z., Xia T., Liu M., Liu Z., Zhou H., Zhang H., Zhai X, & Jin B. (2024). lincRNA00907 promotes NASH progression by targeting miRNA-942-5p/TAOK1. Aging (Albany NY), 16(8), 6868-6882.
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Publication 2024
2
RNA Transfection of BHK Cells
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BHK cells were transfected with RNA using Lipofectamine 2000, based on the manufacturer's protocol (Invitrogen). The RNA was mixed with Opti-Mem (Gibco), with 1 µg (suitable for a 24-well transfection) made up to 50 µL. The Lipofectamine 2000 was diluted 1 in 10 in Opti-Mem to make an equal volume of Lipofectamine 2000-Opti-Mem mix (per RNA sample). Each was incubated at room temperature for 5 minutes, before the RNA/Opti-Mem solution was mixed with an equal volume of the Lipofectamine 2000/Opti-Mem solution and incubated for a further 15 minutes at room temperature. The medium was aspirated from cell monolayers and replaced with the RNA transfection mix, along with sufficient Opti-Mem to cover the cells and prevent them from drying out. The medium was replaced after two hours with an appropriate volume of 1% VGM.
Neil C., Newman J., Stonehouse N.J., Rowlands D.J., Belsham G.J., & Tuthill T.J. (2024). The pseudoknot region and poly-(C) tract comprise an essential RNA packaging signal for assembly of foot-and-mouth disease virus.
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3
Knockdown of STAT3 and VAV3 in L02 cells
Small interfering RNA (siRNA)-to knock down STAT3 (si-STAT3), VAV3 siRNA (si-VAV3) and negative control NC (si-NC) were designed and synthesized by RiboBio (Guangzhou, China). L02 cell lines were transfected with the siRNAs using Lipofectamine 2000 (Thermo Fisher, USA). Cells were seeded in complete medium approximately 12h before transfection. Then, siRNAs mixed with Lipofectamine 2000 were added to the cells with fresh Opti-MEM medium (gibco, USA). SiRNAs were transfected at a concentration of 50 nM. After 6 h, the medium containing siRNAs and Lipofectamine 2000 was replaced with complete medium.
Jiang Y., Luo P., Cao Y., Peng D., Huo S., Guo J., Wang M., Shi W., Zhang C., Li S., Lin L, & Lv J. (2024). The role of STAT3/VAV3 in glucolipid metabolism during the development of HFD-induced MAFLD. International Journal of Biological Sciences, 20(6), 2027-2043.
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Publication 2024
4
TPX2 Knockdown and MMP13 Overexpression
For TPX2 knockdown, cell transfection was conducted with small interfering RNA (siRNA) targeting TPX2 or negative control (NC) by Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). For overexpression of MMP13, cells were transiently transfected with overexpression plasmid encoding MMP13 or empty vector through Lipofectamine 2000 reagent.
Yu J., Wang W., Jiang Z, & Liu H. (2024). TPX2 upregulates MMP13 to promote the progression of lipopolysaccharide-induced osteoarthritis. PeerJ, 12, e17032.
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Publication 2024
5
NFE2L3 Modulation in Gastric Cancer
Plasmids (pcDNA3.1) were purchased from Shanghai Genechem. Plasmids were transfected into AGS via Lipofectamine 2000 (ThermoFisher). siRNA (siNFE2L3#1, siNFE2L3#2, siNFE2L3#3) purchased from RIBOBIO and transfected into HGC-27 using Lipofectamine 2000. qRT-PCR was applied to detect the efficiency of NFE2L3 overexpression or knockdown.
The sequences of siRNA were as follows:
siNFE2L3#1 GCATGACCTTTATCATGAT
siNFE2L3#2 GTATGACCTTGACATAAAT
siNFE2L3#3 GTCAGGATGTGAATCTTCA
Li F, & Wen Z. (2024). Identification roles of NFE2L3 in digestive system cancers. Journal of Cancer Research and Clinical Oncology, 150(3), 150.
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Publication 2024

Top products related to «Lipofectamine 2000»

1
Lipofectamine 2000 by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Italy, Switzerland, Australia, Spain, Belgium, Denmark, Singapore, India, Netherlands, Sweden, New Zealand, Portugal, Poland, Israel, Lithuania, Hong Kong, Argentina, Ireland, Austria, Czechia, Cameroon, Taiwan, Province of China, Morocco
Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
2
Lipofectamine 2000 reagent by Thermo Fisher Scientific
Sourced in United States, China, Germany, Canada, United Kingdom, Japan, France, Italy, Australia, Switzerland, Spain, Netherlands, Singapore, Cameroon, Colombia, Denmark, Lithuania
Lipofectamine 2000 reagent is a cationic lipid-based transfection reagent used for the delivery of nucleic acids, such as DNA and RNA, into eukaryotic cells. It facilitates the uptake of these molecules by the cells, enabling efficient gene expression or gene silencing studies.
3
Dual luciferase reporter assay system by Promega
Sourced in United States, China, Germany, United Kingdom, Switzerland, Japan, France, Italy, Spain, Austria, Australia, Hong Kong, Finland
The Dual-Luciferase Reporter Assay System is a laboratory tool designed to measure and compare the activity of two different luciferase reporter genes simultaneously. The system provides a quantitative method for analyzing gene expression and regulation in transfected or transduced cells.
4
Lipofectamine 2000 transfection reagent by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Canada, Austria, Japan, Netherlands, Sweden, Spain, France, Switzerland, Italy, Lithuania
Lipofectamine 2000 is a cationic lipid-based transfection reagent used for the introduction of nucleic acids, such as DNA and RNA, into a variety of eukaryotic cell types. It facilitates the uptake and delivery of these molecules into the cells.
5
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
6
Opti mem by Thermo Fisher Scientific
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Opti-MEM is a cell culture medium designed to support the growth and maintenance of a variety of cell lines. It is a serum-reduced formulation that helps to reduce the amount of serum required for cell culture, while still providing the necessary nutrients and growth factors for cell proliferation.
7
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
8
Lipofectamine rnaimax by Thermo Fisher Scientific
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Lipofectamine RNAiMAX is a transfection reagent designed for efficient delivery of small interfering RNA (siRNA) and short hairpin RNA (shRNA) into a wide range of cell types. It is a cationic lipid-based formulation that facilitates the uptake of these nucleic acids into the target cells.
9
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
10
Dual luciferase reporter assay kit by Promega
Sourced in United States, China, Germany, United Kingdom, Switzerland, Finland, Italy, Japan
The Dual-Luciferase Reporter Assay Kit is a laboratory tool that allows for the measurement and quantification of two different reporter luciferase enzymes in a single sample. The kit provides the necessary reagents to perform this dual-reporter analysis, which can be used to study gene expression and regulatory elements.

More about "Lipofectamine 2000"

Lipofectamine 2000 is a widely used transfection reagent for efficiently delivering genetic material, such as DNA and RNA, into a variety of cell lines.
This powerful tool is often employed in gene expression studies, reporter assays, and other molecular biology applications.
The Lipofectamine 2000 reagent forms liposomes that can encapsulate and transport nucleic acids into target cells, facilitating their uptake and expression.
To optimize transfection protocols, researchers can utilize the PubCompare.ai platform, which leverages machine learning to analyze and compare the effectiveness of Lipofectamine 2000 protocols from scientific literature, preprints, and patents.
PubCompare.ai provides data-driven insights to help researchers improve their experimental outcomes and maximize the potential of the Lipofectamine 2000 reagent.
By using this AI-powered platform, scientists can discover the most effective Lipofectamine 2000 protocols, enhancing reproducibility and research accuracy.
In addition to Lipofectamine 2000, researchers may also employ other related reagents and assays, such as the Dual-Luciferase Reporter Assay System, Lipofectamine RNAiMAX, and various cell culture media like FBS, Opti-MEM, and DMEM.
These complementary tools and materials can further support successful gene expression studies and transfection experiments.
By harnessing the power of AI and the wealth of available data, PubCompare.ai empowers researchers to optimize their Lipofectamine 2000 protocols, leading to more robust and reliable experimental results.
Experieence the benefits of data-driven protocol optimization and discover the full potential of this versatile transfection reagent.