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Lucigenin

Lucigenin is a luminescent compound used in bioluminescence assays to detect and quantify superoxide.
It emits light when oxidized by superoxide, allowing for the measurement of this important reactive oxygen species.
Lucigenin has been widely utilized in research on oxidative stress, inflammation, and cell signaling pathways.
Optimizing Lucigenin-based protocols is crucial for accurate and reproducible results in these areas of study.
PubCompare.ai's AI-driven platform can help researchers easily locate relevant protocols from literature, preprints, and patents, while providing intelligent comparisons to identify the best methoods and products.
This enhaned reporducibility and accuracy in Lucigenin studies.

Most cited protocols related to «Lucigenin»

Cardiac Function in Nox2 Transgenic Mice

Cited 14 times

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Publication 2015

Animals Biological Assay Caffeine Calcium calyculin A Chemiluminescence DNA, Complementary Homo sapiens Left Ventricles Lucigenin malachite green Mice, Laboratory Mice, Transgenic Myocardium Myocytes, Cardiac myosin light chain 2 Pressure Protein Phosphatase Psychological Inhibition Sarcomeres Sodium Student Systole Transients

Bioluminescent Imaging of ROS

Cited 9 times

Luminol (5-amino-2,3-dihydro-1,4-phthalazinedione) and lucigenin (bis-N-methylacridinium nitrate) are used to detect reactive oxygen species (ROS). Luminol bioluminescence detects mainly to neutrophil myeloperoxidase (MPO) activity, and lucigenin bioluminescence detects NADPH oxidase activity and extracellular superoxide production of macrophages (39 (link),40 (link)). Na-luminol and lucigenin (Santa Cruz Biotechnology) were dissolved in phosphate buffered saline to form 20-mg/ml and 2.5-mg/ml stock solutions, respectively. Anesthetized mice were injected intraperitoneally with 150 mg/kg luminol (days 0, 1, 2, and 4) or 25 mg/kg lucigenin (days 0, 2, 6, and 10). Bioluminescence imaging was performed 10 minutes postinjection using an IVIS Lumina II. Acquisition time was 60 seconds, F-stop 1, Binning 8. Data were analyzed and ROIs were applied; luminescence was expressed as total radiance (photons/second/cm²/steradian) within the ROIs.

Botz B., Bölcskei K., Kereskai L., Kovács M., Németh T., Szigeti K., Horváth I., Máthé D., Kovács N., Hashimoto H., Reglődi D., Szolcsányi J., Pintér E., Mócsai A, & Helyes Z. (2014). Differential Regulatory Role of Pituitary Adenylate Cyclase–Activating Polypeptide in the Serum-Transfer Arthritis Model. Arthritis & Rheumatology (Hoboken, N.j.), 66(10), 2739-2750.

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Publication 2014

10,10'-dimethyl-9,9'-biacridinium Lucigenin Luminescence Luminol Macrophage Mice, House NADPH Oxidase Neutrophil Nitrates Peroxidase Phosphates Reactive Oxygen Species Saline Solution Superoxides

Fungal Immune Response Protocol

Cited 8 times

Luminol, Lucigenin, Isoluminol, HRP Type VI, PMA, SOD from bovine erythrocytes and zymosan were obtained from Sigma (St Louis, MO). FCS, HBSS, H₂DCF-DA were from Invitrogen Molecular Probes (Oregon). DMEM was purchased from PAA (Vienna, Austria), anti-mouse antibodies CD16/CD32, CD11b-FITC, CD11c-APC, F4/80-PE-Cy5 were obtained from BD Bioscience (Mountain View, CA). Rich medium (YPD) and synthetic complete were prepared essentially as described (Kaiser et al., 1994 ). BMDM media are composed of DMEM, 10% heat-inactivated FCS, 20% l-conditioned medium. mDC media are composed of DMEM, 10% heat-inactivated FCS, 10% X-conditioned medium. C. albicans strains were grown at 30°C in YPD medium overnight, diluted to an OD₆₀₀ = 0.2 the next morning, grown to the logarithmic growth phase and used for the experiment unless indicated otherwise. For the preparation of mature filaments, an overnight culture of C. albicans was diluted 1:10 in YPD + 10% FCS and grown at 37°C for 3–4 h. For experiments requiring stimulation of macrophages with filaments, an aliquot of each culture was pelleted and the dry weight was determined by routine procedures. Aliquots of cultures equalling the indicated dry weights of yeast or filaments were used for experiments. Typically, 4 × 10⁴ yeast cells correspond to 1 μg dry weight.

Frohner I.E., Bourgeois C., Yatsyk K., Majer O, & Kuchler K. (2009). Candida albicans cell surface superoxide dismutases degrade host-derived reactive oxygen species to escape innate immune surveillance. Molecular Microbiology, 71(1), 240-252.

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Publication 2009

Anti-Antibodies Bos taurus Candida albicans Cells Culture Media, Conditioned Cytoskeletal Filaments Erythrocytes Fluorescein-5-isothiocyanate Hemoglobin, Sickle isoluminol ITGAM protein, human Lucigenin Luminol Macrophage Molecular Probes Mus Strains Yeasts Zymosan

Cardioprotective Effects of LXR Agonists in Acute Myocardial Infarction

Cited 6 times

This investigation conformed to the National Institutes of Health Guidelines on the Use of Laboratory Animals, and was approved by the Institute’s Animal Ethics Committee. Experiments were performed on wild type (WT) C57BL/6 and LXR-deficient [LXRα-, LXRβ-, or LXRα/β double-knockout (KO)] male mice. Pharmacologic experiments employed LXR endogenous agonist 22(R)-hydroxycholesterol [22(R)-HC] and synthetic agonist GW3965.^{14 (link), 15 (link)} In the acute MI/R protocol, reperfusion commenced for 24 hours following 30 minutes of ischemia. Mice were randomly assigned to the following groups: sham, vehicle, 22(R)-HC (20 mg/kg), or GW3965 (20 mg/kg) by intraperitoneal injection (IP) 15 minutes before reperfusion. To observe the long-term cardioprotective effect of LXRα/β dual agonists, mice were randomly assigned to one of the following groups: sham, vehicle, 22(R)-HC (20 mg/kg), or GW3965 (20 mg/kg) by IP injection 15 minutes before reperfusion, and daily following reperfusion for 4 weeks. Other experiments were designed for MI/R outcome determination in LXRα-, β-, and α/β double-deficiency mice and their WT littermates. Myocardial apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) technique and cardiac caspase-3 activity. Myocardial infarct size was determined by Evans blue-TTC double staining methods.^{16 (link)} In-vivo cardiac function was determined by echocardiography.^{17 (link)} Viable myocardium was assessed via micro-positron emission tomography/computed tomography (micro-PET/CT) scanning. Myocardial reactive oxygen species generation was measured by confocal microscopy via in-situ dehydroetidium (DHE) stain or lucigenin-enhanced chemiluminescence. Myocardial nitrative stress was assessed by nitrotyrosine content. In-vivo knockdown of cardiac-specific LXRα or LXRβ expression was achieved by intramyocardial delivery of siRNA. In-vivo cardiac gene overexpression was achieved by adenoviral-encoded LXRα or LXRβ transfection. An expanded Methods appears in the Data Supplement.

He Q., Pu J., Yuan A., Lau W.B., Gao E., Koch W.J., Ma X.L, & He B. (2014). Activation of LXRα but not LXRβ Protects Against Myocardial Ischemia/Reperfusion Injury. Circulation. Heart failure, 7(6), 1032-1041.

Publication 2014

3-nitrotyrosine Adenoviruses agonists Animal Ethics Committees Animals, Laboratory Apoptosis Caspase 3 Chemiluminescence deoxyuridine triphosphate Dietary Supplements DNA Nucleotidylexotransferase Echocardiography Evans Blue Genes GW 3965 Heart Hydroxycholesterols Injections, Intraperitoneal Ischemia Longterm Effects Lucigenin Males Mice, House Microscopy, Confocal Myocardial Infarction Myocardium Obstetric Delivery Reactive Oxygen Species Reperfusion RNA, Small Interfering Scan, CT PET Stains Transfection

Angiotensin II and Noradrenaline Infusion in Nox2 Tg Mice

Cited 5 times

All animal experiments were conducted in accordance with the UK Home Office Animals (Science Procedures) Act 1986 (HMSO UK). Endothelium-targeted Nox2-overexpressing (Nox2 Tg) mice on C57BL/6 background were generated by our laboratory as described previously.14 (link) For Ang II or noradrenaline infusion, mice anesthetized with isoflurane received osmotic mini-pumps (Alzet Corp) delivering saline, Ang II at 1 mg⋅kg⁻¹⋅d⁻¹, or noradrenaline at 10 mg⋅kg⁻¹⋅d⁻¹ for 3, 5, 14, or 28 days. Systolic blood pressure was measured with the Visitech tail-cuff system in conscious mice. Primary endothelial cells were isolated from lung, macrophages from peritoneal lavage, and VSMCs from aorta. Terminally anesthetized mice were injected with vascular cell adhesion molecule-1 (VCAM-1)/P-selectin or IgG-conjugated microparticles of iron oxide (MPIO) via the left ventricle as described previously.15 (link) Ex vivo high-resolution magnetic resonance imaging (MRI) and 3-dimensional reconstruction were performed as described previously.16 (link) ROS production was quantified with dihydroethidium high-performance liquid chromatography and lucigenin chemiluminescence.
See the online-only Data Supplement for a full description of materials and methods.

Fan L.M., Douglas G., Bendall J.K., McNeill E., Crabtree M.J., Hale A.B., Mai A., Li J.M., McAteer M.A., Schneider J.E., Choudhury R.P, & Channon K.M. (2014). Endothelial Cell–Specific Reactive Oxygen Species Production Increases Susceptibility to Aortic Dissection. Circulation, 129(25), 2661-2672.

Publication 2014

ANGPT1 protein, human Animals Aorta Cell-Derived Microparticles Chemiluminescence Consciousness Dietary Supplements dihydroethidium Endothelial Cells Endothelium ferric oxide High-Performance Liquid Chromatographies Isoflurane Left Ventricles Lucigenin Lung Macrophage Macrophages, Peritoneal Mice, Inbred C57BL Mus Norepinephrine Osmosis Peritoneal Lavage Reconstructive Surgical Procedures Saline Solution SELP protein, human Systolic Pressure Tail Vascular Cell Adhesion Molecule-1

Most recents protocols related to «Lucigenin»

NADPH Oxidase Activity Assay

The enhanced lucigenin-derived chemiluminescence method was adopted to measure the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and ROS level in aortic tissues or A7r5 cells. Both dark-adapted 100 μM NADPH and 5 μM lucigenin were used to trigger the photon emission and the background chemiluminescence was detected by a luminometer (Turner, CA, USA). The data were presented as the mean of light unit (MLU)/min/mg protein.

Wang H.Y., Wang F.Z., Chang R., Wang Q., Liu S.Y., Cheng Z.X., Gao Q., Zhou H, & Zhou Y.B. (2023). Adrenomedullin Improves Hypertension and Vascular Remodeling partly through the Receptor-Mediated AMPK Pathway in Rats with Obesity-Related Hypertension. International Journal of Molecular Sciences, 24(4), 3943.

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Publication 2023

Aorta Cells Chemiluminescence Lucigenin NADP NADPH Oxidase Precipitating Factors Tissues Tumor Necrosis Factor Ligand Superfamily Member 14

Assessing Aortic Superoxide Levels

Superoxide levels in thoracic and abdominal aorta rings were assessed by lucigenin-enhanced chemiluminescence as previously documented [25 (link)]. Samples were added to polypropylene cuvettes with PBS and lucigenin (5 μmol/L). After 10 min of dark adaptation, basal chemiluminescence was measured for 5 min using a luminometer (FB15, Zylux, Maryville, TN, USA). For measurement of NOX activity, luminescence was assessed using NADH (0.2 and 1 mmol/L, ST358, Beyotime, Shanghai, China) or NADPH (0.2 and 1 mmol/L, ST360, Beyotime) as substrates. The source of superoxide was determined by treating samples with apocynin (targeting NOX, 100 µmol/L, 178385, Sigma-Aldrich), L-NAME (targeting eNOS, 100 µmol/L) or oxypurinol (targeting XO, 100 µmol/L, O6881, Sigma-Aldrich) for 30 min prior to chemiluminescence measurement. Relative light unit (RLU) values were normalized to the surface area of each vessel.

Li Q., Du Y., Xiang P., Chen G., Qian X., Li S., Mao Y., Ling W, & Wang D. (2023). Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets. Nutrients, 15(4), 832.

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Publication 2023

acetovanillone Aortas, Abdominal Blood Vessel Chemiluminescence Chemiluminescent Assays Dark Adaptation Light Lucigenin Luminescence NADH NADP NG-Nitroarginine Methyl Ester NOS3 protein, human Oxypurinol Polypropylenes Superoxides

Measuring NOX Activity in Brain Microvessels

NOX activity was assessed as described previously [30 (link)]. The brain microvasculature samples containing phenylmethylsulfonyl fluoride and protease inhibitor cocktail (Thermo Fisher Scientific; 20 μL) were added to a 96-well luminescence plate containing 6.25 μmol/L of lucigenin. The reaction was initiated by an addition of nicotinamide adenine dinucleotide phosphate (100 μmol/L). NOX activity was measured by the change in luminescence recorded by the DTX-880 Multimode Detector.

Zhu Y., Haddad Y., Yun H.J., Geng X, & Ding Y. (2023). Induced Inflammatory and Oxidative Markers in Cerebral Microvasculature by Mentally Depressive Stress. Mediators of Inflammation, 2023, 4206316.

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Publication 2023

Brain Lucigenin Luminescence Microvascular Network NADP Phenylmethylsulfonyl Fluoride Protease Inhibitors

Myeloid Cell MPO Activity Quantification

Cited 2 times

MPO activity was assessed by luminol imaging22 (link) of the isolated CD11b⁺Ly6G⁺ and CD11b⁺ myeloid cells; 1×10⁵ isolated myeloid cells were added to 100 mM luminol, 50 µM L-012, or 100 µM lucigenin in colorless DMEM media in a 96-well black walled plate. Cells were immediately imaged for 120 min using the IVIS 100 bioluminescence imaging system (Perkin Elmer/Caliper Life Sciences, MA, USA) at 37°C under 5% CO₂ flow. Typical acquisition parameters were as follows: acquisition time, autoexposure; binning, 8; FOV, 15 cm; f/stop, 1; filter, open; image-image interval, 5 min; total number of acquisitions, 24. Bioluminescence photon flux (photons/s) data were analyzed by ROI measurements with background subtraction in Living Image 4.5 (Perkin Elmer/Caliper Life Sciences); these raw data were imported into Excel (Microsoft, WA, USA), averaged in each individual experiment if done in duplicate or triplicate wells. Total photon flux for each experiment subtracted the total photon flux of reporter alone controls at each time point. For the CD11b⁺ myeloid cells, the photon flux was normalized to 1000 CD11b⁺ cells quantified by FACS. For luminol, the area under the curve was calculated from images taken at 5–60 min. For L-012, the area under the curve was calculated from images taken at 5–90 min and lucigenin was quantified using images taken at 5–25 min. The area under the curve for each reporter was taken from n=3 independent isolation experiments.

Liu T.W., Gammon S.T., Yang P., Ma W., Wang J, & Piwnica-Worms D. (2023). Inhibition of myeloperoxidase enhances immune checkpoint therapy for melanoma. Journal for Immunotherapy of Cancer, 11(2), e005837.

Publication 2023

Cells isolation ITGAM protein, human L 012 Lucigenin Luminol Myeloid Cells

Reagents for Oxidative Burst Assay

Luminol (sodium salt), lucigenin (N, N′-dimethyl-9,9′-biacridinium dinitrate), phobol 12-myristate 13-acetate (PMA), G418, casein (from bovine milk, sodium salt), and 4-aminobenzoic hydrazide (4-ABAH, myeloperoxidase inhibitor-1; Calbiochem, EMD Millipore) were purchased from Sigma-Aldrich (Sigma-Aldrich, MO, USA). L-012 sodium salt was purchased from Wako Chemicals USA (Richmond, VA, USA). Verdiperstat, AZD5904, and hydroxypropyl-β-cyclodextrin (HP-β-CD) were purchased from MedChemExpress (NJ, USA). Anti-CTLA-4 (9D9), αPD-1 (RMP1-14), CD8α (2.43), CD4⁺ (GK1.5), NK1.1 (PR136), and polyclonal rat IgG control were obtained from Bio X cell (NH, USA). Luminol sodium salt was dissolved in sterile phosphate-buffered saline (PBS) to a final concentration of 50 mg/mL or 100 mg/mL and stored at −20°C. L-012 powder was dissolved in sterile double-distilled water (ddH₂0) to a final concentration of 20 mM and stored at −20°C. 4-ABAH was dissolved in sterile ddH₂O heated to 65°C to a final concentration of 2 mg/mL.44 (link) Verdiperstat and AZD5904 were dissolved in sterile DMSO to a final concentration of 10 mM and stored at −20°C; AZD5904—5% DMSO, 15% HP-β-CD w/v, 200 µL intraperitoneal injection daily. Verdiperstat—4% DMSO,15% HP-β-CD w/v, 300 µL intraperitoneal injection daily. Nine grams of casein was dissolved in 100 mL PBS, pH7.2 containing 0.9 mM CaCl₂ and 0.5 mM MgCl₂.45 (link)

Publication 2023

Acetate antibiotic G 418 Bos taurus Caseins Cells Cyclodextrins Cytotoxic T-Lymphocyte Antigen 4 Hydrazide Hypromellose Injections, Intraperitoneal L 012 Lucigenin Luminol Magnesium Chloride Milk, Cow's Myristate Peroxidase Phosphates Powder Saline Solution Sodium Sodium Chloride Sterility, Reproductive Sulfoxide, Dimethyl

Top products related to «Lucigenin»

Lucigenin by Merck Group

Sourced in United States, Germany, France, China, Switzerland, Japan, United Kingdom

Lucigenin is a fluorescent compound used in analytical chemistry and biochemistry as a chemiluminescent indicator. It is commonly employed in assays for the detection and quantification of superoxide radicals.

Nadph by Merck Group

Sourced in United States, Germany, Sao Tome and Principe, China, United Kingdom, France, Czechia, Spain, India, Italy, Canada, Macao, Japan, Portugal, Brazil, Belgium

NADPH, or Nicotinamide Adenine Dinucleotide Phosphate, is a cofactor essential for various cellular processes. It plays a crucial role in enzymatic reactions, serving as an electron donor in oxidation-reduction reactions. NADPH is a key component in several metabolic pathways, including biosynthesis, antioxidant defense, and energy production.

Luminol by Merck Group

Sourced in United States, Germany, France, China, Switzerland, Canada, Brazil, Sao Tome and Principe, Italy, United Kingdom, Macao

Luminol is a chemiluminescent compound used in analytical chemistry and forensic science. It emits light when oxidized, which can be used to detect the presence of certain substances, such as blood. The core function of Luminol is to serve as a reagent in various chemical and biological assays.

Microplate reader by Agilent Technologies

Sourced in United States, China, Germany, Japan, United Kingdom, France, Australia, Switzerland, Ireland, Canada, India, Mongolia, Hong Kong

The Microplate reader is a versatile laboratory instrument used to measure and analyze the optical properties of samples in microplates. It is designed to quantify absorbance, fluorescence, or luminescence signals from various assays and applications.

Lumat lb 9507 by Berthold Technologies

Sourced in Germany, United States, Japan, France, Belgium, Switzerland

The Lumat LB 9507 is a luminometer designed for sensitive detection and quantification of luminescent signals. It is capable of measuring various types of luminescence, including bioluminescence, chemiluminescence, and fluorescence.

Apocynin by Merck Group

Sourced in United States, Germany, Italy, United Kingdom, Sao Tome and Principe, Macao, Brazil, China, France, Austria

Apocynin is a chemical compound used in laboratory settings. It functions as an inhibitor of the enzyme NADPH oxidase, which plays a role in the production of reactive oxygen species. Apocynin is commonly utilized in research applications to investigate the involvement of oxidative stress in various biological processes.

Luminometer by Berthold Technologies

Sourced in Germany, United States, China

The Luminometer is a laboratory instrument used to measure and quantify the amount of light produced by a bioluminescent or chemiluminescent reaction. It serves as a sensitive and accurate tool for detecting and analyzing luminescent signals generated in various biological and chemical processes.

Penicillin streptomycin by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia

Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

Microplate luminometer by Hidex

Sourced in Finland

The Microplate Luminometer is a laboratory instrument designed to measure the light emission from samples in microplates. It is used to detect and quantify luminescent signals, which can be generated by various biological and chemical processes.

Bis n methylacridinium nitrate by Merck Group

Sourced in United States, United Kingdom

Bis-N-methylacridinium nitrate is a chemical compound used as a laboratory reagent. It is a bright yellow crystalline solid that is soluble in water and organic solvents. The compound is commonly used in various analytical and experimental procedures, but a detailed description of its core function would require further information to maintain an unbiased and factual approach.

What is Lucigenin and how is it used in research?

Lucigenin is a luminescent compound that is widely used in bioluminescence assays to detect and quantify superoxide, an important reactive oxygen species. When oxidized by superoxide, Lucigenin emits light, allowing for the measurement of oxidative stress, inflammation, and cell signaling pathways. Optimizing Lucigenin-based protocols is crucial for accurate and reproducible results in these areas of study.

What are some common challenges in using Lucigenin for research?

One of the main challenges in using Lucigenin is ensuring accurate and reproducible results. Factors such as assay conditions, sample preparation, and interfering substances can significantly impact the reliability of Lucigenin-based measurements. Researchers must carefully optimize their protocols to minimize these sources of variability and ensure the validity of their findings.

Are there different types or variations of Lucigenin that researchers should be aware of?

Yes, there are several different forms of Lucigenin that can be used in research. The most common are Lucigenin (bis-N-methylacridinium nitrate) and its derivatives, such as Lucigenin C and Lucigenin C methiodide. These variations can have slightly different properties, including sensitivity, specificity, and stability, which can impact their suitability for different experimental applications.

What are some specific applications of Lucigenin in research?

Lucigenin is widely used in a variety of research fields, including: 1. Oxidative stress and inflammation: Measuring superoxide production in cells and tissues to understand the role of oxidative stress in disease pathogenesis. 2. Cell signaling: Monitoring the activation of signaling pathways that involve superoxide, such as those related to immune function and cell proliferation. 3. Drug discovery: Screening for compounds that modulate superoxide levels or antioxidant defenses as potential therapeutic targets.

How can PubCompare.ai help researchers optimize the use of Lucigenin in their studies?

PubCompare.ai's AI-driven platform can assist researchers in optimizing their use of Lucigenin in several ways: 1. Efficiently screen protocol literature: PubCompare.ai allows you to quickly and easily find relevant protocols from the literature, preprints, and patents related to Lucigenin-based assays. 2. Leverage AI to pinpoint critical insights: The platform's intelligent analysis can highlight key differences in protocol effectiveness, enabling you to identify the best methods for your specific research goals and ensure reproducibility and accuracy. 3. Identify the most effective Lucigenin protocols: PubCompare.ai can help you locate the protocols that have been most successful in achieving reliable and reproducible results, saving you time and effort in your research.

More about "Lucigenin"

Lucigenin, a luminescent compound, is widely used in bioluminescence assays to detect and quantify superoxide, a key reactive oxygen species involved in oxidative stress, inflammation, and cell signaling pathways.
This chemical emits light when oxidized by superoxide, allowing for the measurement of this important biological marker.
Optimizing Lucigenin-based protocols is crucial for accurate and reproducible results in these areas of study.
Researchers can leverage PubCompare.ai's AI-driven platform to easily locate relevant protocols from literature, preprints, and patents, while receiving intelligent comparisons to identify the best methods and products.
This can enhance the reproducibility and accuracy of Lucigenin studies, which are vital for understanding the role of NADPH oxidases, Apocynin, and other factors in oxidative stress and related signaling cascades.
Luminol, another luminescent compound, is sometimes used in conjunction with Lucigenin to provide a more comprehensive analysis of reactive oxygen species.
Microplate readers and Lumat LB 9507 luminometers are common instrumentation used to measure the light output in Lucigenin-based assays.
By incorporating best practices and cutting-edge tools, researchers can unlock deeper insights into the complex interplay between superoxide, inflammation, and cellular processes, leading to improved understanding and potential therapeutic interventions.
Visit PubCompare.ai today to discover how our platform can streamline your Lucigenin research and enhance the reproducibility and accuracy of your studies in this exciting field of study.