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LY 294002

LY 294002 is a powerful phosphoinositide 3-kinase (PI3K) inhibitor, widely used in biomedical research to investigate the PI3K signaling pathway.
This compound has been extensively studied for its effects on cell growth, survival, and metabolism, making it a valuable tool for understanding the role of PI3K in various cellular processes.
Researchers can leverage PubCompare.ai's AI-driven platform to easily locate and compare protocols involving LY 294002 from literature, preprints, and patents, optimizing their research effeciently.

Most cited protocols related to «LY 294002»

1
Quantifying Macropinocytosis and Endocytosis
Cited 18 times
To measure macropinocytosis, serum-starved A431 cells grown on coverslips and placed in Chamlid chambers were incubated with 0.5 mg/ml TMR-dextran and, where noted, stimulated with 100–200 ng/ml EGF in the indicated buffer for 10 min at 37°C. Cells were washed and both DIC and red fluorescence images of live cells were acquired. Where indicated, the following inhibitors were used: 10 µM latrunculin B, 100 µM LY294002, 1 mM amiloride, or 10 µM HOE-694. In the case of latrunculin B and LY294002 the cells were preincubated with the inhibitors at 37°C for 30 min before EGF addition. Macropinocytosis was quantified as the number of cells containing macropinosomes in the cells outlining each island.
Endocytosis was assessed by incubating the cells with 50 µg/ml Alexa 546–conjugated transferrin in the indicated buffer for 15 min at 37°C, after which the cells were placed on ice and acid washed with 0.2 M acetic acid in 150 mM NaCl and PBS to remove exofacial fluorescence. The cells were then fixed and mounted on slides, and red fluorescence was imaged and quantified.
Koivusalo M., Welch C., Hayashi H., Scott C.C., Kim M., Alexander T., Touret N., Hahn K.M, & Grinstein S. (2010). Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling. The Journal of Cell Biology, 188(4), 547-563.
Publication 2010
Acetic Acid Acids Amiloride Buffers Cells Dextran Endocytosis Erythrocytes Fluorescence HOE 694 inhibitors latrunculin B LY 294002 Serum Sodium Chloride Transferrin
2
Directed Differentiation of hESCs
Cited 13 times
The hESC lines H1, Hes2-ICN1-ER™ and HES3-RUNX1CGFP/w -EGFP were maintained on irradiated mouse embryonic fibroblasts in hESC media as described previously2 (link). For differentiation, hESCs were cultured on Matrigel-coated plasticware (BD Biosciences) for 24 hours, followed by embryoid body generation, as described previously2 (link). Briefly, the undifferentiated hESCs were dissociated with Tryp-LE (GIBCO) treatment, followed by scraping. Aggregates were resuspended in StemPro-34 (Invitrogen), supplemented with penicillin/streptomycin (10 ng/mL), L-glutamine (2mM), ascorbic acid (1mM), monothioglycerol (MTG, 4×10−4M; Sigma), transferrin (150 μg/mL) (referred to as “supplemented StemPro-34”) and BMP-4 (10 ng/mL), and cultured in 6-well low-cluster plates (Corning) in a volume of 2 mL per well. Following 24 hours, bFGF was added to a final concentration of 5ng/mL. At day 2, the developing EBs were harvested, washed and resuspended in supplemented StemPro-34 with BMP-4, bFGF and either CHIR99021 (3 μM, Stemgent), IWP2 (3μM, Tocris Bioscience) or Activin-A (1ng/ml) as indicated. After 24 hours, the EBs were again harvested and resuspended in supplemented StemPro-34 containing VEGF (15 ng/mL), bFGF (5 ng/mL), IL-6 (10 ng/mL) and IL-11 (5 ng/mL) and cultured for 48 hours. At this stage, the EBs were fed with 2ml of the same media containing also SCF (50 ng/mL final), IGF-1 (25 ng/mL final) and EPO (2 U/mL final) and cultured until day 8. Cultures were maintained in a 5% CO2/5% O2/90% N2 environment. All recombinant factors are human and were purchased from R&D Systems. Where indicated 4-OHT (1 μM, Sigma), GSI (L-685,458, 10 μM, R&D Systems) PI3Ki (LY294002, 10 μM R&D System), MEKi (PD0325901, 1 μM, Stemgent) were included.
Ditadi A., Sturgeon C.M., Tober J., Awong G., Kennedy M., Phillips A., Azzola L., Ng E.S., Stanley E., French D.L., Cheng X., Gadue P., Speck N., Elefanty A.G, & Keller G. (2015). HUMAN DEFINITIVE HAEMOGENIC ENDOTHELIUM AND ARTERIAL VASCULAR ENDOTHELIUM REPRESENT DISTINCT LINEAGES. Nature cell biology, 17(5), 580-591.
Publication 2015
4,17 beta-dihydroxy-4-androstene-3-one activin A Ascorbic Acid BMP4 protein, human Chir 99021 Embryo Embryoid Bodies Fibroblasts Glutamine HES2 protein, human Homo sapiens Human Embryonic Stem Cells IGF1 protein, human Interleukin-11 L 685458 LY 294002 matrigel Mus PD-0325901 Penicillins Streptomycin thioglycerol Transferrin Vascular Endothelial Growth Factors
3
Allantois Dissection and Culture for Vascular Analysis
Cited 13 times
Allantoises were dissected from E8.5 embryos and cultured for 18 hours in 8-well µ-slides (Ibidi) coated with bovine fibronectin (Sigma) in DMEM (Gibco) supplemented with 10% fetal calf serum (Invitrogen) in a humidified tissue culture incubator at 37°C and 5% CO2 (Sanyo). Inhibitors were made up as per manufacturer's instructions and added into the growth medium when setting up the culture. Control explants were vehicle treated. Inhibitors were obtained from Calbiochem and used at the following concentrations: LY294002, 10 µM; Y-27632, 10 µM. Allantois explants were fixed with 4% paraformaldehyde, followed by wholemount staining with rat anti-VE-cadherin antibody (BD Biosciences) together with rat anti-endomucin. Overlapping pictures of the explants were taken with an inverted epifluorescence microscope (Olympus CellR), making sure that no area was over-or underexposed. Photographs were assembled using the panorama function of Photoshop software.
Zudaire E., Gambardella L., Kurcz C, & Vermeren S. (2011). A Computational Tool for Quantitative Analysis of Vascular Networks. PLoS ONE, 6(11), e27385.
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Publication 2011
Allantois Antibodies, Anti-Idiotypic Bos taurus CDH5 protein, human Culture Media Embryo Endomucins Fetal Bovine Serum FN1 protein, human inhibitors LY 294002 Microscopy paraform Tissues Y 27632
4
Pluripotent Stem Cell Differentiation to Hepatic and Pancreatic Endoderm
Cited 9 times
hESCs (H9 from WiCell, Maddison, WI, USA) and hIPSCs (BBHX8, A1ATD-1, JRO1D; University of Cambridge, Cambridge, UK) [16 (link)] were passaged weekly using collagenase IV and maintained in chemically defined medium (CDM) supplemented with activin A (10 ng/ml) and FGF2 (12 ng/ml), as described previously [17 (link)]. Differentiation was carried out as described in Fig. 1 and is explained in detail in the electronic supplementary material (ESM) Methods.

Protocols to generate hepatic and pancreatic endoderm from hESCs and hIPSCs. Successive culture conditions driving differentiation of pluripotent stem cells towards pancreatic endoderm and hepatic endoderm. A, activin; ADV, advanced DMEM; B, BMP; CMRL, Connaught Medical Research Laboratories medium; CYCP, cytochrome P450; DAPT, N-(N-[3,5-diflurophenylacetyl]-l-alanyl)-S-phenylglycine t-butyl ester; F, FGF; HGF, hepatocyte growth factor; Ly, LY294002; Nog, noggin; OSM, oncostatin M

Cho C.H., Hannan N.R., Docherty F.M., Docherty H.M., Joåo Lima M., Trotter M.W., Docherty K, & Vallier L. (2012). Inhibition of activin/nodal signalling is necessary for pancreatic differentiation of human pluripotent stem cells. Diabetologia, 55(12), 3284-3295.
Publication 2012
1,2-dilinolenoyl-3-(4-aminobutyryl)propane-1,2,3-triol activin A Activins alpha 1-Antitrypsin Deficiency Collagenase Cytochrome P450 Endoderm Esters Fibroblast Growth Factor 2 Hepatocyte Growth Factor Human Embryonic Stem Cells Human Induced Pluripotent Stem Cells LY 294002 noggin protein OSM protein, human Pancreas Pluripotent Stem Cells
5
Expression Plasmids for TGF-β Signaling
Cited 9 times

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Publication 2009
1-Phosphatidylinositol 3-Kinase 4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide ADAM17 protein, human Amino Acids Epitopes LY 294002 MG 132 N-((2-(hydroxyaminocarbonyl)methyl)-4-methylpentanoyl)-3-(2'-naphthyl)alanylalanine, 2-aminoethylamide Open Reading Frames PD 98059 Phosphotransferases Plasmids sulfosuccinimidyl 6-(biotinamido)hexanoate TGFBR1 protein, human TGFBR2 protein, human Transforming Growth Factor beta U 0126

Most recents protocols related to «LY 294002»

1
LSCC Cell Line Characterization and Inhibition
The human LSCC cell lines of AMC-HN-8 and TU177 were purchased from Beijing Beina Chuanglian Institute of Biotechnology (Beijing, China). The AMC-HN-8 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM), containing 10% foetal bovine serum (FBS). The TU177 cells were cultured in RPMI-1640 medium, supplemented with 10% FBS. All of the above cell culture reagents were provided by GIBCO (Thermo Fisher Scientific, Inc). Accordingly, the culture of two cells was performed in a humidified incubator with 5% CO2 atmosphere at 37°C (Thermo Fisher Scientific, Inc). The PI3K inhibitor (LY294002) and mTOR inhibitor (Rapamycin) were purchased from MCE (New Jersey, USA). LSCC cells were incubated with LY294002 or Rapamycin to perform functional assays and Western blot assay.
Yu L., Cao H., Yang J.W., Meng W.X., Yang C., Wang J.T., Yu M.M, & Wang B.S. (2023). HDAC5-mediated PRAME regulates the proliferation, migration, invasion, and EMT of laryngeal squamous cell carcinoma via the PI3K/AKT/mTOR signaling pathway. Open Medicine, 18(1), 20230665.
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Publication 2023
Atmosphere Biological Assay Cell Culture Techniques Cell Lines Cells Culture Media Eagle Fetal Bovine Serum Homo sapiens LY 294002 MTOR Inhibitors Phosphatidylinositol 3-Kinases Sirolimus Western Blot
2
PRAME-Driven Tumor Growth in Xenograft Model
An animal xenograft model was employed to examine the effect of PRAME on tumor growth in vivo. A total of 20 male nude (BALB/c nude Crlj) mice (age: 6 weeks old; weight: 18−22 g) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Animal Permit NO: SCXK [Jing] 2016-0011). The nude mice were randomly assigned into four groups, including the empty vector group; the transiently transfected PRAME group; the stably transfected PRAME group; and the LY294002 (an inhibitor of PI3K) group (n = 5 in the respective group). 1 × 107 AMC-HN-8 cells transfected with overexpressed PRAME were mixed with 100 μl culture medium free of FBS, followed by the subcutaneous injection into the right flanks of the BALB/c nude mice. The tumor volume was documented every 3 days with a caliper by the formula: (A × B2)/2, where A denotes the maximum diameter and B represents the diameter perpendicular to A. Four weeks later, all nude mice were sacrificed, and the tumor xenografts were harvested immediately and weighed. The animal study was conducted at the Institute of Otolaryngology of Hebei Medical University following the NIH guidelines. All procedures of animal experiments were approved by the Hebei Medical University and were conducted in agreement with international guidelines for the maintenance and care of laboratory animals.
Yu L., Cao H., Yang J.W., Meng W.X., Yang C., Wang J.T., Yu M.M, & Wang B.S. (2023). HDAC5-mediated PRAME regulates the proliferation, migration, invasion, and EMT of laryngeal squamous cell carcinoma via the PI3K/AKT/mTOR signaling pathway. Open Medicine, 18(1), 20230665.
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Publication 2023
Animal Model Animals Animals, Laboratory Cells Cloning Vectors Culture Media Heterografts LY 294002 Males Mice, Inbred BALB C Mice, Nude Mus Neoplasms Phosphatidylinositol 3-Kinases Rivers Subcutaneous Injections
3
Modulation of GRP78 Signaling in Primary Murine Macrophages
Primary MC from C57Bl/6 mice were isolated for culture using Dynabeads. They were cultured in DMEM (1,000 mg/L or 5.6 mM glucose) supplemented with 20% FBS, 100 µg/mL streptomycin, and 100 µg/mL penicillin at 37°C in 95% O2, 5% CO2. Cells were serum-deprived in 0.5% FBS for 24 h prior to treatment with HG (30 mM) with or without inhibitors of csGRP78 signaling: the C-terminus targeting GRP78 antibody C38 (2 µg/mL) (Munro and Pelham, 1987 (link); De Ridder et al., 2012 (link); Trink et al., 2022 (link)) or vaspin (100 ng/mL) (Nakatsuka et al., 2012 (link); Nakatsuka et al., 2013 (link); Abdolahi et al., 2022 (link)), or the following PI3K/Akt inhibitors: wortmannin (100 ng/mL), LY294002 (20 µM), and Akt Inhibitor VIII (10 µM).
Trink J., Ahmed U., O’Neil K., Li R., Gao B, & Krepinsky J.C. (2023). Cell surface GRP78 regulates TGFβ1-mediated profibrotic responses via TSP1 in diabetic kidney disease. Frontiers in Pharmacology, 14, 1098321.
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Publication 2023
1,3-dihydro-1-(1-((4-(6-phenyl-1H-imidazo(4,5-g)quinoxalin-7-yl)phenyl)methyl)-4-piperidinyl)-2H-benzimidazol-2-one Cells Glucose Glucose Regulated Protein 78 kDa Immunoglobulins inhibitors LY 294002 Mice, Inbred C57BL Penicillins PIK3CB protein, human Serum Streptomycin Wortmannin
4
ATP Regulation of FGF21 in Muscle
To determine the effect of ATP (Adenosine 5′-triphosphate, Sigma-Aldrich Corp, St. Louis, MO, USA) on either FGF21 protein or mRNA levels, isolated fiber cultures or FDB muscles were previously serum-starved for 2 h (DMEM culture medium without horse serum). Subsequently, muscles were stimulated with exogenous ATP at selected concentrations (0.1-100 µM), for different times (30-360 min). When blockers or inhibitors were used, they were incubated 30 min before and during the stimulation with ATP. Evaluation of changes in mRNA and protein levels in response to ATP was performed in the presence of 100 μM Suramin (Sigma-Aldrich Corp, St. Louis, MO, USA), 25 μM Nifedipine (Sigma-Aldrich Corp, St. Louis, MO, USA), 100 nM Rapamycin (Sigma-Aldrich Corp, St. Louis, MO, USA), 50 μM LY294002 (Cell Signaling Technology, Danvers, MA, EEUU), 10 μM Akt VIII (Sigma-Aldrich Corp, St. Louis, MO, USA) 30 μM Cycloheximide (Sigma-Aldrich Corp, St. Louis, MO, USA) or 0.5 μM actinomycin-D (Sigma-Aldrich Corp, St. Louis, MO, USA).
Arias-Calderón M., Casas M., Balanta-Melo J., Morales-Jiménez C., Hernández N., Llanos P., Jaimovich E, & Buvinic S. (2023). Fibroblast growth factor 21 is expressed and secreted from skeletal muscle following electrical stimulation via extracellular ATP activation of the PI3K/Akt/mTOR signaling pathway. Frontiers in Endocrinology, 14, 1059020.
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Publication 2023
Adenosine Triphosphate Culture Media Cycloheximide Dactinomycin Equus caballus fibroblast growth factor 21 Fibrosis inhibitors LY 294002 Muscle Tissue Nifedipine Proteins RNA, Messenger Serum Sirolimus Suramin
5
Macrophage Polarization Assay by Western Blot
To estimate the polarization of macrophages induced by PMA, lipopolysaccharide(LPS), interferon-γ(IFN-γ), IL-4, and IL-13, the expression of the markers of M1-type macrophages, CD86 and inducible nitric oxide synthase(iNOS), and the markers of M2-type macrophages, CD163 and IL-10, were detected by Western blotting. The influence of AFP on the expression of these marker proteins and PI3K/Akt signaling pathway-related proteins was analyzed in M0-type macrophages. M0 macrophages were infected with the AFP-expressed lentiviral vectors and treated with the PI3K inhibitor Ly294002 (final concentration:20 μM) for 48 h. The expression of these proteins was analyzed by Western blotting. Briefly, these proteins were probed with the following primary antibodies: mouse anti-CD86 (1:500), anti-iNOS (1:500), anti-CD163 (1:500), anti-IL-10 (1:500), anti-β-actin (1:1000), rabbit anti-PI3K (1:400), anti-Akt (1:400), or anti-p-Akt(Ser473) (1:400) (all from eBioscience and Abcam Inc.). The detailed procedure has been previously described (20 (link), 28 (link)).
Zhang M., Liu K., Zhang Q., Xu J., Liu J., Lin H., Lin B., Zhu M, & Li M. (2023). Alpha fetoprotein promotes polarization of macrophages towards M2-like phenotype and inhibits macrophages to phagocytize hepatoma cells. Frontiers in Immunology, 14, 1081572.
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Publication 2023
Actins Antibodies CD163 protein, human Cloning Vectors IL10 protein, human Interferon Type II Interleukin-13 LY 294002 Macrophage Macrophage Activation Mus NOS2A protein, human PIK3CG protein, human Proteins Rabbits Signal Transduction Pathways

Top products related to «LY 294002»

1
Ly294002 by Merck Group
Sourced in United States, Germany, United Kingdom, China, Sao Tome and Principe, Macao, Italy, Canada, Switzerland, Japan, France, Israel, Spain, Morocco
LY294002 is a chemical compound that functions as a specific inhibitor of phosphoinositide 3-kinase (PI3K). It is commonly used in laboratory research settings to investigate the role of PI3K signaling pathways.
2
Ly294002 by Cell Signaling Technology
Sourced in United States, Germany, United Kingdom, China
LY294002 is a selective and potent inhibitor of phosphoinositide 3-kinase (PI3K). It functions by blocking the ATP-binding site of the PI3K enzyme, thereby inhibiting its catalytic activity.
3
Ly294002 by Selleck Chemicals
Sourced in United States, China, Germany, United Kingdom, Japan
LY294002 is a chemical compound used in research laboratories. It is a potent and selective inhibitor of the PI3 kinase enzyme.
4
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
5
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
6
Pd98059 by Merck Group
Sourced in United States, Germany, United Kingdom, China, Macao, Sao Tome and Principe, Italy, Japan
PD98059 is a chemical compound used as a laboratory reagent. It functions as a specific and potent inhibitor of the mitogen-activated protein kinase (MAPK) pathway.
7
Ly294002 by MedChemExpress
Sourced in United States, China
LY294002 is a potent and selective phosphoinositide 3-kinase (PI3K) inhibitor. It inhibits the activity of PI3K, a key enzyme involved in cell signaling pathways.
8
Lipofectamine 2000 by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Italy, Switzerland, Australia, Spain, Belgium, Denmark, Singapore, India, Netherlands, Sweden, New Zealand, Portugal, Poland, Israel, Lithuania, Hong Kong, Argentina, Ireland, Austria, Czechia, Cameroon, Taiwan, Province of China, Morocco
Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
9
Rapamycin by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Macao, Israel, Sao Tome and Principe, Spain, Canada, Japan, France, Switzerland, Senegal, Belgium
Rapamycin is a macrolide compound isolated from the bacterium Streptomyces hygroscopicus. It functions as an immunosuppressant and has anti-proliferative effects.
10
Sb203580 by Merck Group
Sourced in United States, Germany, Macao, United Kingdom, China, Japan, Spain, Switzerland, Canada, Sao Tome and Principe, Sweden, Italy
SB203580 is a lab equipment product manufactured by Merck Group. It is a pyridinyl imidazole compound that functions as a selective inhibitor of p38 mitogen-activated protein kinase (MAPK).

More about "LY 294002"

Exploring the Power of LY 294002: A Versatile PI3K Inhibitor for Biomedical Research LY 294002, a potent phosphoinositide 3-kinase (PI3K) inhibitor, has become a staple in the world of biomedical research.
This compound, widely used to investigate the PI3K signaling pathway, offers valuable insights into cell growth, survival, and metabolism.
Researchers can leverage PubCompare.ai's AI-driven platform to easily locate and compare protocols involving LY 294002 from literature, preprints, and patents, optimizing their research efficiently.
PI3K, a crucial enzyme in cellular processes, is responsible for regulating a variety of functions, including cell proliferation, differentiation, and survival.
LY 294002 has emerged as a powerful tool for researchers to study the role of PI3K in these processes, making it a valuable asset in understanding the underlying mechanisms of various cellular activities.
In addition to LY 294002, other commonly used compounds in biomedical research include FBS (Fetal Bovine Serum), DMSO (Dimethyl Sulfoxide), PD98059, Lipofectamine 2000, Rapamycin, and SB203580.
These compounds, each with their own unique properties and applications, can be used in conjunction with LY 294002 to create a comprehensive research approach.
PubCompare.ai's AI-driven platform empowers researchers to navigate the vast landscape of biomedical literature, preprints, and patents, seamlessly locating and comparing protocols involving LY 294002.
This streamlined access to relevant information allows researchers to identify the most effective protocols and products, optimizing their research efforts and accelerating their discoveries.
Explore the versatility of LY 294002 and unlock the potential of your biomedical research with the help of PubCompare.ai's powerful tools and expert insights.
Discover new possibilities and drive your research forward with confidence.