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Lymphoprep

Lymphoprep is a density gradient medium used for the isolation and purification of mononuclear cells, such as lymphocytes, from whole blood or other biological samples.
This AI-driven comparison tool allows researchers to easily locate and evaluate Lymphoprep protocols from literature, preprints, and patents, ensuring reproducible and accurate research.
By optimizing Lymphoprep protocols, scientists can experince the power of AI-assisted procedure selection for their cell separation needs, leading to more reliable and efficient lymphocyte isolation.

Most cited protocols related to «Lymphoprep»

1
High-Quality Plasmodium falciparum SNP Calling
Cited 28 times
DNA was extracted directly from blood samples taken from patients at admission, after leukocyte depletion to minimize contamination from human DNA. Leukocyte depletion was achieved by CF11 filtration for most samples10 (link) or alternatively by Lymphoprep density gradient centrifugation (Axis-Shield) followed by Plasmodipur filtration (Euro-Diagnostica)36 (link) or by Plasmodipur filtration alone. Genomic DNA was extracted using the QIAamp DNA Blood Midi or Maxi kit (Qiagen), and the quantities of human and Plasmodium DNA were determined by fluorescence analysis using a Qubit instrument (Invitrogen) and multispecies quantitative PCR(qPCR) using the Roche LightCycler 480 II system, as described previously11 (link). Samples with >50 ng of DNA and <80% human DNA contamination were selected for sequencing on the Illumina HiSeq platform following the manufacturer's standard protocols37 (link). Paired-end sequencing reads of 200–300bp in length were obtained, generating approximately 1Gb of read data per sample.
Polymorphism discovery, quality control and sample genotyping followed a process described elsewhere11 (link). Short sequence reads from 3,281 P. falciparum samples included in the MalariaGEN Plasmodium falciparum Community Project were aligned against the P. falciparum 3D7 reference sequence V3 using the bwa program38 (link) as previously described11 (link), to identify an initial global set of 3,373,632 potential SNPs. This list was then used to guide stringent realignment using the SNP-o-matic algorithm39 (link), to reduce misalignment errors. Stringent alignments were then examined by a series of quality filters, with the aim of removing alignment artifacts and their sources. In particular, the following were removed: (i) noncoding SNPs; (ii) SNPs where polymorphisms had extremely low support (<10 reads in 1 sample); (iii) SNPs with more than 2 alleles, with the exception of loci known to be important for drug resistance, which were manually verified to not have artifacts; (iv) SNPs where coverage across samples was lower than the 25th percentile or higher than the 95th percentile of coverage in coding SNPs (these thresholds were determined from an analysis of artifact incidence); (v) SNPs located in regions of relatively low uniqueness11 (link); (vi) SNPs where heterozygosity levels were found to be inconsistent with the heterozygosity distribution at the SNP's allele frequency; and (vii) SNPs where the genotype could not be established in at least 70% of samples. These analyses produced a final list of 681,587 high-quality SNPs in the 14 chromosomes of the nuclear genome, whose genotypes were used for analysis in this study.
All samples were genotyped at each high-quality SNP by a single allele, on the basis of the number of reads observed for the two alleles at that position in the sample. At positions with fewer than five reads, the genotype was set to undetermined (no call was made). At all other positions, the sample was determined to be heterozygous if both alleles were each observed in more than two reads; otherwise, the sample was called as homozygous for the allele observed in the majority of reads. For the purposes of estimating allele frequencies and genetic distances, a within-sample allele frequency (fw) was also assigned to each valid call. For heterozygous calls, fw was estimated as the ratio of the non-reference read count to the reference read count; homozygous calls were assigned fw = 0 when called with the reference allele and fw = 1 when called with the non-reference allele.
For specific analyses that required no genotype missingness in our data set, we produced a set of genotypes where missing calls (with coverage <5 reads) were assigned a genotype by simple imputation. First, we considered missing calls where the two flanking positions (on each side) had valid genotypes, imputing with the allele that most frequently appeared at the same position between the same flanking alleles in the full sample set. Finally, remaining samples with missing genotypes were assigned with the most common allele at that position in their population.
Miotto O., Amato R., Ashley E.A., MacInnis B., Almagro-Garcia J., Amaratunga C., Lim P., Mead D., Oyola S.O., Dhorda M., Imwong M., Woodrow C., Manske M., Stalker J., Drury E., Campino S., Amenga-Etego L., Thanh T.N., Tran H.T., Ringwald P., Bethell D., Nosten F., Phyo A.P., Pukrittayakamee S., Chotivanich K., Chuor C.M., Nguon C., Suon S., Sreng S., Newton P.N., Mayxay M., Khanthavong M., Hongvanthong B., Htut Y., Han K.T., Kyaw M.P., Faiz M.A., Fanello C.I., Onyamboko M., Mokuolu O.A., Jacob C.G., Takala-Harrison S., Plowe C.V., Day N.P., Dondorp A.M., Spencer C.C., McVean G., Fairhurst R.M., White N.J, & Kwiatkowski D.P. (2015). Genetic architecture of artemisinin-resistant Plasmodium falciparum. Nature genetics, 47(3), 226-234.
Publication 2015
2
ELISPOT Assay for Peptide-Specific T Cells
Cited 21 times
96-well polyvinylidene difluoride backed plates (MAIP S 45; Millipore, Bedford, MA) were coated with 15 μg/ml of anti–IFN-γ mAb 1-D1K (Mabtech, Stockholm, Sweden) overnight at 4°C. Plates were then washed 6 times with RPMI-1640 and blocked with RPMI supplemented with l-glutamine, penicillin, and 10% heat-inactivated pooled human AB serum (R10) for 1 h. PBMCs were separated from heparinized whole blood on LYMPHOPREP (Nycomed Pharma AS, Oslo, Norway), washed 3 times, and resuspended in R10. PBMCs were added in 100 μl R10/well to the precoated plates. Input cell numbers were 5 × 105/well, in duplicate wells. For assays performed in parallel with limiting dilution analyses (LDAs), duplicate wells with 5 x 105 and 2.5 × 105 PBMCs/well were used.
Detection of peptide-specific T cells from freshly isolated PBMCs is complicated by the fact that the target cells used for peptide presentation elicit responses from T cells of other specificities. Heterologous B cell lines (BCLs) register strong responses from alloreactive T cells, whereas autologous BCLs result in potent EBV-specific responses. Allo-specific and EBV-specific responses were circumvented by using the autologous fresh PBMCs themselves to present peptide. Peptides were added to a final concentration of 2 μM. Where the cell line CIR-A2.01 was used to present the M1 58–66 peptide to fresh PBMCs, the cell line was prepulsed with a 2 μM concentration of peptide in R10 for 1 h, and then washed 3 times.
Assays were incubated for 6 h at 37°C, 5% CO2, but some experiments were run overnight (14 h) for convenience. Assays were arrested by shaking off the contents and washing 6 times with PBS 0.05% Tween 20 (Sigma Chemical Co., St. Louis, MO). Next, 100 μl of 1 μg/ml of the biotinylated anti–IFN-γ mAb 7-B6-1 biotin (Mabtech, Stockholm, Sweden) was added. After 3 h of incubation, plates were washed six times more and a 1:1,000 dilution of streptavidin alkaline phosphatase conjugate (Mabtech) was added to the wells and the plates incubated at room temperature for a further 2 h. Next, wells were again washed 6 times and 100 μl of chromogenic alkaline phosphatase substrate (Bio Rad Labs., Hercules, CA), diluted 1:25 with deionized water, was added. After 30 min, the colorimetric reaction was terminated by washing with tap water and plates were air dried.
Lalvani A., Brookes R., Hambleton S., Britton W.J., Hill A.V, & McMichael A.J. (1997). Rapid Effector Function in CD8+ Memory T Cells. The Journal of Experimental Medicine, 186(6), 859-865.
Publication 1997
3
Comparative Analysis of Leukocyte and Platelet Removal Methods for P. vivax Cultures
Cited 13 times
Due to the limited quantity of P. vivax IRBC healthy donor blood was used for the initial side-by-side comparisons of leukocytes and platelet removal methods. Ten ml of whole blood were collected onto Lithium heparin from five healthy donors by venepuncture. The whole blood was centrifuged at 500 g for 5 min at room temperature. The plasma supernatant was removed, but the buffy coat fraction containing white blood cells (WBC) was added back to the red blood cell (RBC) pellet. The RBC+WBC mix from each donor was divided into five two ml portions to which an equal volume of RPMI was added with gentle mixing. The first tube was used as the control for this study. The 2nd tube was centrifuged again as above and the PBS supernatant and buffy coat carefully removed from the packed RBC, this tube was the 'Buffy Coat Removal' treatment. The 3rd tube was loaded into a five ml syringe (the plunger removed) and mounted onto a Plasmodipur™ filter (Euro-Diagnostica®) that was pre-rinsed with a sterile PBS solution. Then gentle pressure was applied to the syringe attached to the Plamodipur unit and the filtered RBC/PBS (50% haematocrit) mix was collected as the 'Plasmodipur' treatment. The 4th and 5th tubes were added to PBS-wetted CF11 columns. The filtered 4th sample was then collected as the 'CF11' treatment. The filtered 5th sample was then added to another unused CF11 column (pre-wetted with 5 ml of PBS). The double filtered 5th sample was the 'CF11x2' treatment. In addition to the above samples, three more healthy volunteers were recruited, and 8 ml of whole blood were collected onto Lithium heparin. This blood was processed as above and divided into four 2 ml samples. The first and 2nd tubes were processed as described above for the control and the 'CF11' treatments, respectively. The contents of the 3rd tube were layered over 2 ml of Lymphoprep™ (Greiner Bio-One®) and centrifuged at 600 g for 20 min at 20°C, the leukocytes that banded at the interface were removed using a pipette, and the supernatant RBC fraction was the 'Lymphoprep' treatment. The 4th tube was processed as above for the 'Lymphoprep' treatment, but then subsequently processed using the 'Plasmodipur' treatment protocol described above. This final sample was called the 'Lymphoprep and Plasmodipur' treatment.
Please note; unless specifically stated, the term 'CF11 filtration' involved only a single CF11 filter, as opposed to 'CF11x2' which involved processing the sample using two CF11 columns.
Complete blood counts of the control samples and the suspensions obtained from the six treatments were conducted using an Automated Hematology Analyzer (Model pocH-100i, Sysmex Company) and by microscopic examination (x100 oil immersion) of Giemsa-stained thick (250 fields) and thin smears (450 fields). The data from the thin smears were used for differential lymphocyte counts.
Sriprawat K., Kaewpongsri S., Suwanarusk R., Leimanis M.L., Lek-Uthai U., Phyo A.P., Snounou G., Russell B., Renia L, & Nosten F. (2009). Effective and cheap removal of leukocytes and platelets from Plasmodium vivax infected blood. Malaria Journal, 8, 115.
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Publication 2009
BLOOD Blood Platelets Cells Complete Blood Count Donor, Blood Donors Erythrocytes Filtration Healthy Volunteers Heparin Leukocytes Lithium Lymphocyte Count lymphoprep Microscopy Plasma Pressure Sterility, Reproductive Submersion Syringes Tissue Donors Treatment Protocols Venipuncture Volumes, Packed Erythrocyte
4
Isolation and Differentiation of Primary Immune Cells
Cited 8 times
Human primary CD4+ T cells and monocytes were obtained from peripheral blood mononuclear cells (PBMCs) of healthy volunteer donors. Briefly, PBMCs were isolated using Lymphoprep (Axis-Shield) and monocytes were obtained first by positive selection using CD14 MicroBeads (Miltenyi Biotec). CD4+ T cells were then isolated from the remaining cells using the CD4+ T cell isolation kit II (Miltenyi Biotec). The purity of isolated cell populations was always >90% as judged by flow cytometric analysis of specific markers (CD4 and CD3 for CD4+ T cells, or CD14 for monocytes). After 3 h in serum-free RPMI 1640 medium, monocytes were further differentiated into macrophages by culture for 4 to 6 days in RPMI 1640 supplemented with 10% fetal bovine serum (FBS; Gibco), and 100 ng/ml granulocyte-macrophage colony stimulating factor (GM-CSF; R&D Systems). CD4+ T cells were stimulated for 24 h with 1 μg/ml phytohemagglutinin (PHA; Oxoid) and 50 U/ml interleukin-2 (IL-2; BD Pharmingen) prior to culture in the presence or absence of IFNα. Human monocytic THP-1 and U937 cells, and lymphoid Jurkat, CEM, CEM-SS, or HUT78 cells were grown in RPMI 1640 medium, supplemented with 10% FBS. The differentiation of THP-1 and U937 cells (PMA-THP-1 and PMA-U937, respectively) was achieved by 24 h stimulation with 100 ng/ml phorbol-12-myristate-13-acetate (PMA; SIGMA-Aldrich). Human glioblastoma U87-MG and 293T cells were maintained in complete DMEM plus 10% FBS. When indicated, IFNα (Universal type 1 IFN, PBL InterferonSource) was added at 500 to 1000 U/ml for 24 h prior to RNA extraction, immunoblotting, or virus infection.
U87-MG/CD4/CXCR4 (i.e., U87-MG cells modified to express HIV-1 receptors) were obtained in one of two ways: 1) for experiments where viral DNA accumulation was analysed (i.e., Fig 3), cells were transduced with a MLV-based retroviral vector containing a CD4-IRES-CXCR4 expression cassette (derived from pMIGR1 48 by introduction of CD4 as an XhoI-EcoRI fragment and CXCR4 as an NcoI-SalI fragment to replace GFP); 2) for the other experiments (e.g., Fig 1B, 2, 4E), cells were transduced with a combination of an HIV-1-based lentiviral vector expressing a CD4-IRES-puromycin N-acetyltransferase (called herafter puromycinR) expression cassette (pRRL.sin.cPPT.CMV/CD4-IRES-puromycinR.WPRE, obtained by replacement of CMV/eGFP cassette in pRRL.sin.cPPT.CMV/eGFP.WPRE by a CD4-IRES-puromycinR BamHI-Sall fragment, using overlapping PCR) and an MLV-based retroviral vector containing a CXCR4-IRES-neomycinR expression cassette (modified from NG/neo by adding CXCR4 as an XhoI-EcoRI fragment2 (link)). In the latter case, transduced cells were selected with 1 μg/ml puromycin plus 100 μg/ml G418. 293T-TetR:KRAB cells (expressing a fusion of the tetracyclin repressor protein - TetR - with a transcriptional repressor domain, the Krüppel-associated box protein, KRAB) were obtained via 293T transduction with an HIV-1-based lentiviral vector expressing a TetR:KRAB-IRES-puromycinR expression cassette (obtained by replacing CD4 from pRRL.sin.cPPT.CMV/CD4-IRES-puromycinR.WPRE with the TetR:KRAB coding sequence, amplified from pptTRKrab49 (link)) and selected for a few days with 1 μg/ml puromycin. Fresh 293T-TetR:KRAB cells were generated every ~10 days.
U87-MG/LTR-Luc indicator cells were obtained by transducing U87-MG cells with an HIV-1-based lentiviral vector containing the firefly luciferase reporter gene under the control of HIV-1 long terminal repeat (LTR) (pK365, provided by John Kappes 50). These cells mirror the widely used TZM-bl indicator cells50 (link) and express luciferase in response to expression of HIV and SIV Tat trans-activator proteins.
Goujon C., Moncorgé O., Bauby H., Doyle T., Ward C.C., Schaller T., Hué S., Barclay W.S., Schulz R, & Malim M.H. (2013). Human MX2 is an interferon-induced post-entry inhibitor of HIV-1 infection. Nature, 502(7472), 10.1038/nature12542.
Publication 2013
5
Isolation and Expansion of EBV-Specific Cytotoxic T Cells
Cited 8 times
Heparinized whole blood (50 ml) was diluted 1:1 in RPMI 1640 medium and separated by centrifugation on lymphoprep (Nycomed Pharma, Oslo, Norway). Peripheral blood mononuclear cells (PBMCs) were harvested from the interface, washed, and cryopreserved within 3 h of venesection. Aliquots of PBMCs were used for the establishment of an EBV-transformed LCL either by spontaneous transformation with the donor's own virus strain or by addition of exogenous EBV (B95.8 strain); for this, the culture medium was RPMI 1640 + 2 mM glutamine + 10% vol/vol FCS, initially supplemented with 0.1 μg/ml cyclosporin A. The proportion of cells spontaneously entering lytic cycle in such lines was determined by indirect immunofluorescence staining using the BZLF1specific mAb BZ-1 (31 (link)) and the virus capsid antigen–specific mAb V3 (32 (link)).
Acute IM PBMC effectors for use in ex vivo cytotoxicity assays were thawed in IL-2–enriched medium (see below) and used either directly or after initial depletion of CD16+ NK cells. For the latter, PBMCs were incubated for 30 min at 4°C with 5 μl antiCD16 IgM mAb (leu 11b; Becton Dickinson, San Jose, CA)/106 cells, washed once, and incubated for 1 h at 37°C in IL-2–enriched medium containing 5:1 vol/vol rabbit complement (C0999f; Harlan Sera-Lab, Loughborough, U.K.), and then washed twice before use in the cytotoxicity assay.
Primary polyclonal CTL lines were established by stimulating thawed IM PBMCs on day 0 and weekly thereafter with the autologous LCL at an effector/stimulator ratio of 4:1 in RPMI 1640 + 2 mM glutamine + 10% vol/vol FCS, 1% vol/vol human serum, 30% vol/vol supernatant from the IL-2–secreting cell line MLA-144 (MLA-SN), and 100 U/ml of recombinant IL-2 (hereafter called IL-2–enriched medium). Primary CTL clones were established by limiting dilution of thawed IM PBMCs directly into IL-2–enriched medium at 0.3–100 cells/0.2 ml round-bottomed well in the presence of irradiated preactivated allogeneic PBMC feeders (106/ml) either alone, with irradiated autologous LCL cells (105/ml), or with the addition of the anti-CD3 mAb (OKT3; Unipath, Basingstoke, U.K.) to a final concentration of 50 ng/ml. In each case, growing microcultures were further expanded by transfer into 2-ml wells using the same stimulation protocol as before. Feeder cells were from pooled fresh buffy coats (National Blood Service, Birmingham, U.K.) and were incubated with PHA at 10 μg/ml for 1 h, and then washed five times before irradiation and use. In some cases, CTL clones were also established from 14-d cultures of the polyclonal CTL line or from limiting dilution cultures by reseeding at 0.3–3 cells/well using the conditions outlined above. Memory CTL clones were established as previously described (33 (link)). In brief, PBMCs from postconvalescent IM donors or from long-term virus carriers were stimulated with the autologous LCL at a responder/stimulator ratio of 40:1, and then restimulated 10 d later at a responder/ stimulator ratio of 4:1. Cloning took place 4 d later by seeding at 0.3–3 cells/well using the conditions outlined above. All primary and memory CTL clones were screened for CD4 and CD8 expression by indirect immunofluorescence staining using the CD4-specific mAb 716 and the CD8-specific mAb 707 (Dako, High Wycombe, U.K.).
Steven N.M., Annels N.E., Kumar A., Leese A.M., Kurilla M.G, & Rickinson A.B. (1997). Immediate Early and Early Lytic Cycle Proteins Are Frequent Targets of the Epstein-Barr Virus–induced Cytotoxic T Cell Response. The Journal of Experimental Medicine, 185(9), 1605-1618.
Publication 1997
Antigens, Viral Biological Assay BLOOD Capsid Proteins Cell Lines Cells Centrifugation Clone Cells Cyclosporine Cytotoxin Donors Feeder Cells Glutamine Homo sapiens Indirect Immunofluorescence lymphoprep Memory Muromonab-CD3 Natural Killer Cells PBMC Peripheral Blood Mononuclear Cells Phlebotomy Rabbits Radiotherapy Serum Strains Technique, Dilution Virus

Most recents protocols related to «Lymphoprep»

1
Isolation and Activation of Human PBMCs
Human peripheral blood mononuclear cells (HPBMCs) were obtained from healthy participants at the same institute as mentioned above. Based on the manufacturer's instructions, HPBMCs were harvested via Lymphoprep (Stemcell Technologies, Vancouver, Canada) as previously described.
19 (link) Briefly, the diluted blood was carefully added on the top of Lymphoprep. After centrifuging, the mononuclear cell layer at the plasma: Lymphoprep interface was isolated and washed once with Hanks' Balanced Salt Solution. Typically, 1 × 106 HPBMC was activated with 25 mL ImmunoCult Human CD3/CD28 T Cell Activator (STEMCELL, catalog no. 10971) in 1 mL ImmunoCult‐XF T Cell Expansion Medium (STEMCELL, catalog no. 10981) supplemented with 10 ng/mL IL2 (Peprotech, catalog no. 200‐02).
Guo B., Zheng Q., Jiang Y., Zhan Y., Huang W, & Chen Z. (2024). Long non‐coding RNAFOXD1‐AS1 modulated CTCs epithelial‐mesenchymal transition and immune escape in hepatocellular carcinoma in vitro by sponging miR‐615‐3p. Cancer Reports, 7(3), e2050.
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Publication 2024
2
Isolation of Primary Human Lymphocytes
Buffy coats from healthy blood donors were received from the blood center at the Uppsala University Hospital, Sweden. Peripheral blood mononuclear cells (PBMC) were isolated using SepMate tubes-50 (Stem Cell Technologies) by density gradient centrifugation. Briefly, 10 ml Lymphoprep reagent (Stem Cell Technologies) was added to the tubes followed by addition of blood on top of Lymphoprep. The tubes were then centrifuged at 1200×g for 10 min. Next, cell suspension above the Lymphoprep was collected and PBMCs were washed twice with phosphate-buffered saline (PBS, Thermo Fisher Scientific). For optimal lysis of red blood cells, 5 ml ACK lysis buffer (Thermo Fisher Scientific) was added to the cells and incubated in the dark for 10 min at room temperature followed by centrifugation at 500 × g for 5 min. After that, primary monocytes were removed by an EasySep CD14+ selection kit II (Stem Cell Technology) according to the manufacturer’s instructions. Primary human lymphocytes were stored in −150 °C until use.
Papakyriacou I., Kutkaite G., Rúbies Bedós M., Nagarajan D., Alford L.P., Menden M.P, & Mao Y. (2024). Loss of NEDD8 in cancer cells causes vulnerability to immune checkpoint blockade in triple-negative breast cancer. Nature Communications, 15, 3581.
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Publication 2024
3
Isolation and Cryopreservation of PBMCs
Whole blood samples from participants were collected in ethylenediaminetetraacetic acid-treated tubes and inert separation gel vacuum procoagulant collective tubes. Serum was obtained by centrifugation of procoagulant blood and frozen at −80°C. PBMCs were separated by Lymphoprep (Stemcell Technologies, Canada) centrifugation and frozen at −196°C in liquid nitrogen. Briefly, fresh blood samples were mixed with an identical volume of phosphate-buffered saline (PBS) and carefully placed on the surface of the Lymphoprep separation medium. After centrifugation at 500 × g for 20 min at 28°C, the PBMCs were collected at the interphase and washed with PBS by centrifugation for 10 min at 300 × g.
Qiu Y., Li Z.T., Zeng W., Yang J.L., Tang M.X., Wang Y., Wang H.R., Li Y., Zhan Y.Q., Li S.Q., Zhang J.Q, & Ye F. (2024). Th1 cell immune response in Talaromyces marneffei infection with anti-interferon-γ autoantibody syndrome. Microbiology Spectrum, 12(5), e03646-23.
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Publication 2024
4
Isolation of Human PBMCs
Human PBMCs were purified by lymphoprep (StemCell Technologies, catalog no. 07851) density gradient centrifugation from healthy donor leukocyte cones (purchased from NHS Blood and Transplant, Speke, Liverpool, United Kingdom).
Saulters E.L., Kennedy P.T., Carter R.J., Alsufyani A., Jones T.M., Woolley J.F, & Dahal L.N. (2024). Differential Regulation of the STING Pathway in Human Papillomavirus–Positive and -Negative Head and Neck Cancers. Cancer Research Communications, 4(1), 118-133.
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Publication 2024
5
Tumor and Blood Cell Isolation for Single-cell Analysis
Single-cell tumor and blood suspensions were prepared, as previously described7 (link). In brief, freshly resected tumor samples were mechanically dissociated and digested in HBSS medium with collagenase IV (2.5 mg/ml; Roche) and DNase I (0.2 mg/ml; Worthington Biochemical Corporation) at 37°C for 30 min. Dissociated tumor suspensions were then isolated using Lymphoprep gradient centrifugation. Peripheral blood mononuclear cells were isolated from whole blood using Lymphoprep gradient centrifugation. For samples Mel-T-09, Mel-T-11, Mel-UT-03, Mel-UT-05, Mel-UT-08–13, and Mel-UT-17–18, single-cell suspensions were cryopreserved in GemCell human AB serum (Gemini) with 20% DMSO in liquid nitrogen. The single-cell suspensions were then thawed per 10X Genomics protocols CG000233 and CG000447 for tumor and blood samples, respectively, prior to further processing.
Hafler D., Lu B., Lucca L., Lewis W., Wang J., Nogeuira C., Heer S., Axisa P.P., Buitrago-Pocasangre N., Pham G., Kojima M., Wei W., Aizenbud L., Bacchiocchi A., Zhang L., Walewski J., Chiang V., Olino K., Clune J., Halaban R., Kluger Y., Coyle A., Kisielow J., Obermair F.J, & Kluger H. (2024). Circulating Tumor Reactive KIR+CD8+ T cells Suppress Anti-Tumor Immunity in Patients with Melanoma. Research Square.
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Publication Preprint 2024

Top products related to «Lymphoprep»

1
Lymphoprep by STEMCELL
Sourced in Canada, United Kingdom, United States, France, Germany, Norway, China, Australia, Singapore, Italy
Lymphoprep is a density gradient medium used for the isolation of lymphocytes from whole blood or buffy coat. It separates lymphocytes from other blood cells based on their density.
2
Lymphoprep by Axis-Shield
Sourced in Norway, United Kingdom, United States, Canada, Germany
Lymphoprep is a density gradient medium used for the separation and isolation of mononuclear cells from whole blood or bone marrow. It is designed to facilitate the separation of lymphocytes and other mononuclear cells from erythrocytes and granulocytes.
3
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
4
Rpmi 1640 by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Canada, Japan, Australia, Italy, Switzerland, Belgium, New Zealand, Austria, Netherlands, Israel, Sweden, Denmark, India, Ireland, Spain, Brazil, Norway, Argentina, Macao, Poland, Holy See (Vatican City State), Mexico, Hong Kong, Portugal, Cameroon
RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.
5
L glutamine by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, France, Canada, Switzerland, Italy, Australia, Belgium, China, Japan, Austria, Spain, Brazil, Israel, Sweden, Ireland, Netherlands, Gabon, Macao, New Zealand, Holy See (Vatican City State), Portugal, Poland, Argentina, Colombia, India, Denmark, Singapore, Panama, Finland, Cameroon
L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
6
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
7
Rpmi 1640 medium by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Japan, France, Canada, Australia, Italy, Switzerland, Belgium, New Zealand, Spain, Israel, Sweden, Denmark, Macao, Brazil, Ireland, India, Austria, Netherlands, Holy See (Vatican City State), Poland, Norway, Cameroon, Hong Kong, Morocco, Singapore, Thailand, Argentina, Taiwan, Province of China, Palestine, State of, Finland, Colombia, United Arab Emirates
RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
8
Lymphoprep by Takeda Pharmaceuticals
Sourced in Norway, Switzerland, United Kingdom
Lymphoprep is a sterile density gradient medium used for the isolation of mononuclear cells, such as lymphocytes and monocytes, from whole blood or bone marrow samples. It facilitates the separation of these cells through differential migration during centrifugation.
9
Lymphoprep by Abbott
Sourced in Norway, United States, United Kingdom, Germany
Lymphoprep is a density gradient medium used for the separation and isolation of lymphocytes from whole blood or buffy coat samples. It facilitates the separation of mononuclear cells, including lymphocytes, from erythrocytes and granulocytes.
10
Streptomycin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Australia, Japan, Switzerland, Italy, Belgium, Israel, Austria, Spain, Netherlands, Poland, Brazil, Denmark, Argentina, Sweden, New Zealand, Ireland, India, Gabon, Macao, Portugal, Czechia, Singapore, Norway, Thailand, Uruguay, Moldova, Republic of, Finland, Panama
Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.

More about "Lymphoprep"

Lymphoprep is a density gradient medium widely used for the isolation and purification of mononuclear cells, such as lymphocytes, from whole blood or other biological samples.
This AI-driven comparison tool, PubCompare.ai, allows researchers to easily locate and evaluate Lymphoprep protocols from literature, preprints, and patents, ensuring reproducible and accurate research.
By optimizing Lymphoprep protocols, scientists can experience the power of AI-assisted procedure selection for their cell separation needs, leading to more reliable and efficient lymphocyte isolation.
Lymphoprep is often used in conjunction with other cell culture components like Fetal Bovine Serum (FBS), RPMI 1640 medium, L-glutamine, and Dimethyl Sulfoxide (DMSO) to create a complete cell isolation and culture system.
RPMI 1640 medium is a widely used cell culture medium that provides essential nutrients for the growth and maintenance of a variety of cell types, including lymphocytes.
L-glutamine is a common supplementt added to RPMI 1640 to support cell metabolism and proliferation.
DMSO is often used as a cryoprotectant to preserve cells during freezing and storage.
By leveraging the power of AI-driven protocol comparison and optimization, researchers can streamline their Lymphoprep-based cell isolation workflows, leading to more consistent and reliable results in their lymphocyte-focused studies and applications.