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Lysophosphatidylcholines

Lysophosphatidylcholines are a class of lipid molecules that play crucial roles in cellular signaling, membrane structure, and metabolic processes.
These amphipathic compounds are derived from phosphatidylcholines through the enzymatic removal of one fatty acid, resulting in a single fatty acid chain attached to a glycerophosphocholine backbone.
Lysophosphatidylcholines are involved in a wide range of biological functions, including inflammation, cell proliferation, and lipid homeostasis.
They serve as important intermediates in the biosynthesis and degradation of phospholipids and can act as second messengers, influencing cell behavior and physiological responses.
Accurate and reproducible protocols for the study of lysophosphatidylcholines are essential for advancing our understanding of their roles in health and disease.
PubCompare.ai helps researchers optimize their lysophosphatidylcholine research by identifying the most reliable and efficient protocols from the scientific literature, preprints, and patents, enhancing protocol selection and product identification to ensure reliable and efficeint studies.

Most cited protocols related to «Lysophosphatidylcholines»

1
Lipid Extraction from Diluted Plasma
Cited 53 times
The lipid extraction (adapted from Matyash et al. 23 (link)) was carried out in high grade polypropylene deep well plates. Fifty microliters of diluted plasma (50×) (equivalent of 1 μL of undiluted plasma) was mixed with 130 μL of ammonium bicarbonate solution and 810 μL of methyl tert-butyl ether/methanol (7:2, v/v) solution was added. Twenty-one microliters of internal standard mixture was pre-mixed with the organic solvents mixture. The internal standard mixture contained: 50 pmol of lysophasphatidylglycerol (LPG) 17:1, 50 pmol of lysophosphatic acid (LPA) 17:0, 500 pmol of phosphatidylcholine (PC) 17:0/17:0, 30 pmol of hexosylceramide (HexCer) 18:1;2/12:0, 50 pmol of phosphatidylserine (PS) 17:0/17:0, 50 pmol of phosphatidylglycerol (PG) 17:0/17:0, 50 pmol of phosphatic acid (PA) 17:0/17:0, 50 pmol of lysophposphatidylinositol (LPI 17:1), 50 pmol of lysophosphatidylserine (LPS) 17:1, 1 nmol cholesterol (Chol) D6, 100 pmol of diacylglycerol (DAG) 17:0/17:0, 50 pmol of triacylglycerol (TAG) 17:0/17:0/17:0, 50 pmol of ceramide (Cer) 18:1;2/17:0, 200 pmol of sphingomyelin (SM) 18:1;2/12:0, 50 pmol of lysophosphatidylcholine (LPC) 12:0, 30 pmol of lysophosphatidylethanolamine (LPE) 17:1, 50 pmol of phosphatidylethanolamine (PE) 17:0/17:0, 100 pmol of cholesterol ester (CE) 20:0, 50 pmol of phosphatidylinositol (PI) 16:0/16:0. The plate was then sealed with a teflon-coated lid, shaken at 4°C for 15 min, and spun down (3000 g, 5 min) to facilitate separation of the liquid phases and clean-up of the upper organic phase. Hundred microliters of the organic phase was transferred to an infusion plate and dried in a speed vacuum concentrator. Dried lipids were re-suspended in 40 μL of 7.5 mM ammonium acetate in chloroform/methanol/propanol (1:2:4, v/v/v) and the wells were sealed with an aluminum foil to avoid evaporation and contamination during infusion. All liquid handling steps were performed using Hamilton STARlet robotic platform with the Anti Droplet Control feature for organic solvents pipetting.
Surma M.A., Herzog R., Vasilj A., Klose C., Christinat N., Morin-Rivron D., Simons K., Masoodi M, & Sampaio J.L. (2015). An automated shotgun lipidomics platform for high throughput, comprehensive, and quantitative analysis of blood plasma intact lipids. European Journal of Lipid Science and Technology, 117(10), 1540-1549.
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Publication 2015
1-Propanol Acids Aluminum ammonium acetate ammonium bicarbonate Ceramides Chloroform Cholesterol Cholesterol Esters Diacylglycerol Lipids Lysophosphatidylcholines lysophosphatidylethanolamine lysophosphatidylserine Methanol methyl tert-butyl ether Phosphates Phosphatidylcholines phosphatidylethanolamine Phosphatidyl Glycerol Phosphatidylinositols Phosphatidylserines Plasma Polypropylenes Solvents Sphingomyelins Teflon Triglycerides Vacuum
2
Comprehensive Metabolomics Profiling
Cited 26 times
In total, 163 different metabolites were detected (Table 3 in Online Methods). The metabolomics dataset contains 14 amino acids, hexose (H1), free carnitine (C0), 40 acylcarnitines (Cx:y), hydroxylacylcarnitines (C(OH)x:y), and dicarboxylacylcarnitines (Cx:y-DC), 15 sphingomyelins (SMx:y) and N-hydroxylacyloylsphingosyl-phosphocholine (SM (OH)x:y), 77 phosphatidylcholines (PC, aa=diacyl, ae=acyl-alkyl) and 15 lysophosphatidylcholines. Lipid side chain composition is abbreviated as Cx:y, where x denotes the number of carbons in the side chain and y the number of double bonds. E.g. “PC ae C33:1” denotes an acyl-alkyl phosphatidylcholine with 33 carbons in the two fatty acid side chains and a single double bond in one of them. Full biochemical names are provided in Supplementary Table 4. The precise position of the double bonds and the distribution of the carbon atoms in different fatty acid side chains cannot be determined with this technology. In some cases, the mapping of metabolite names to individual masses can be ambiguous. For example, stereo-chemical differences are not always discernible, neither are isobaric fragments. In such cases, possible alternative assignments are indicated.
Illig T., Gieger C., Zhai G., Römisch-Margl W., Wang-Sattler R., Prehn C., Altmaier E., Kastenmüller G., Kato B.S., Mewes H.W., Meitinger T., de Angelis M.H., Kronenberg F., Soranzo N., Wichmann H.E., Spector T.D., Adamski J, & Suhre K. (2009). A genomewide perspective of genetic variation in human metabolism. Nature genetics, 42(2), 137-141.
Publication 2009
acylcarnitine Amino Acids Carbon Carnitine Fatty Acids Hexoses Lipids Lysophosphatidylcholines Phosphatidylcholines Phosphorylcholine single bond Sphingomyelins
3
Mass Spectrometry Analysis of Lipid Extracts
Cited 25 times
Lipid extracts were dissolved in 60 μl of chloroform/methanol (1:2, v/v) and subjected to mass spectrometric analysis using an LTQ Orbitrap XL instrument (Thermo Fisher Scientific) equipped with a TriVersa NanoMate (Advion Biosciences) as previously described [4 (link),7 (link)]. The 10:1-phase lipid extracts were analyzed by positive ion mode multiplexed FT MS analysis with scan ranges m/z 280-580 (monitoring lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE) species) and m/z 500-1200 (monitoring sphingomyelin (SM), ceramide (Cer), diacylglycerol (DAG), PC, ether-linked PC (PC O-), phosphatidylethanolamine (PE), ether-linked phosphatidylethanolamine (PE O-) and triacylglycerol (TAG) species). The 2:1-phase lipid extracts were analyzed by negative ion mode multiplexed FT MS analysis with scan ranges m/z 370-660 (monitoring lysophosphatidic acid (LPA), lysophosphatidylserine (LPS) and lysophosphatidylinositol (LPI) species) and m/z 550-1700 (monitoring phosphatidic acid (PA), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidylglycerol (PG) and sulfatide (SHexCer) species). All FT MS spectra were acquired in profile mode using a target mass resolution of 100,000 (fwhm), activation of isolation waveforms, automatic gain control at 1e6, max injection time at 250 ms and acquisition of 2 µscans.
Husen P., Tarasov K., Katafiasz M., Sokol E., Vogt J., Baumgart J., Nitsch R., Ekroos K, & Ejsing C.S. (2013). Analysis of Lipid Experiments (ALEX): A Software Framework for Analysis of High-Resolution Shotgun Lipidomics Data. PLoS ONE, 8(11), e79736.
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Publication 2013
Ceramides Chloroform Diacylglycerol Ethyl Ether isolation Lipids lysophosphatidic acid Lysophosphatidylcholines lysophosphatidylethanolamine lysophosphatidylinositol lysophosphatidylserine M 280 Mass Spectrometry Methanol Phosphatidic Acid phosphatidylethanolamine Phosphatidyl Glycerol Phosphatidylinositols Phosphatidylserines Radionuclide Imaging Sphingomyelins Sulfoglycosphingolipids Triglycerides
4
Lipid Annotation and Quantification
Cited 21 times
Lipid classes are: PE, phosphatidylethanolamines; LPE; lyso-phosphatidylethanolamines; PE-O, 1-alkyl-2-acylglycerophosphoethanolamines; PS, phosphatidylserines; PC, phosphatidylcholines; PC-O, 1-alkyl-2-acylglycerophosphocholines; LPC, lysophosphatidylcholines; SM, sphingomyelins; PA, phosphatidic acids; PG, phosphatidylglycerols; PI, phosphatidylinositols; DAG, diacylglycerols; TAG, triacylglycerols; CL, cardiolipins; LCL, triacyl-lysocardiolipins; Cer, ceramides; Chol, cholesterol; CholEst, cholesterol esters.
Individual molecular species are annotated as follows: :/:. For example, PC 18:0/18:1 stands for a phosphatidylcholine comprising the moieties stearic (18:0) and oleic (18:1) fatty acids. If the exact composition of fatty acid or fatty alcohol moieties is not known, the species are annotated as: :. In this way, PC 36:1 stands for a PC species having 36 carbon atoms and one double bond in both fatty acid moieties.
Herzog R., Schwudke D., Schuhmann K., Sampaio J.L., Bornstein S.R., Schroeder M, & Shevchenko A. (2011). A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language. Genome Biology, 12(1), R8.
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Publication 2011
Carbon Cardiolipins Ceramides Cholesterol Cholesterol Esters Diglycerides Fatty Acids Fatty Alcohols Lipids Lysophosphatidylcholines Phosphatidic Acids Phosphatidylcholines Phosphatidylethanolamines Phosphatidylglycerols Phosphatidylinositols Phosphatidylserines Sphingomyelins Triglycerides
5
Membrane Protein Dynamics in a Realistic Plasma Membrane
Cited 12 times
The detailed methods are described in the Supporting Information. In summary, we embedded 10 membrane proteins in
a previously characterized model of the plasma membrane.20 (link) The starting structures of the 10 membrane proteins
simulated in this study were taken from the Protein Data Bank or obtained
from the corresponding publication: aquaporin-1 (AQP1, PDB ID 1J4N);98 (link) prostaglandin H2 synthase (COX1, PDB ID 1Q4G);99 (link) the dopamine transporter (DAT, PDB ID 4M48);44 (link) the epidermal growth factor receptor (EGFR);77 (link) AMPA-sensitive glutamate receptor 2 (GluA2,
PDB ID 3KG2);100 (link) glucose transporter 1 (GluT1, PDB ID 4PYP);101 (link) voltage-dependent Shaker potassium channel 1.2 (Kv1.2,
PDB ID 3LUT,102 (link) residues 32 to 4421 for each monomer); sodium,
potassium pump (Na,K-ATPase, PDB ID 4HYT);103 (link) δ-opioid
receptor (δ-OPR, PDB ID 4N6H);104 (link) and P-glycoprotein
(P-gp, PDB ID 4M1M).105 (link) In each system, four copies of each
protein were included and positioned at a distance of ca. 20 nm from
each other. Proteins were simulated using standard Martini protocols
with minor variations between systems to accommodate system-specific
issues (Supporting Information). The following
lipid classes were included: cholesterol (CHOL), in both leaflets;
charged lipids phosphatidylserine (PS), phosphatidic acid (PA), phosphatidylinositol
(PI), and the PI-phosphate, PI-bisphosphate, and PI-trisphosphate
(PIPs) placed in the inner leaflet; and ganglioside (GM) in the outer
leaflet. The zwitterionic phosphatidylcholine (PC), phosphatidylethanolamine
(PE), and sphingomyelin (SM) lipids were placed in both leaflets,
with PC and SM primarily in the outer leaflet and PE in the inner
leaflet. Ceramide (CER), diacylglycerol (DAG), and lysophosphatidylcholine
(LPC) lipids were also included, with all the LPC in the inner leaflet,
and CER and DAG primarily in the outer leaflet. The details of the
Martini lipids used in this study can be found on the Martini Lipidome
webpage (http://www.cgmartini.nl/index.php/force-field-parameters/lipids) and are described by Ingolfsson et al., and Wassenaar et al.20 (link),106 (link) The exact lipid composition of each system is given in the Supporting Information. The systems are ca. 42
× 42 nm in the membrane plane (x and y), including 4 proteins and ca. 6000 lipids.
Production
runs were performed in the presence of weak position
restraints applied to the protein backbone beads, with a force constant
of 1 kJ mol–1 nm–2, preventing
proteins from associating with each other. Each of the systems has
been simulated for 30 μs, which turned out to be adequate to
obtain convergence of major lipid components in the lipid shells around
the individual copies of the proteins (Supporting Information). Additional control simulations were performed
in the AQP1 system, in order to test the effects of simulation length,
position restraints on the proteins, lipid composition, and water
model on the results of lipid composition near the proteins (Supporting Information).
Simulations were
performed using the GROMACS simulation package
version 4.6.3,107 (link) with the Martini v2.2
force field parameters,62 (link),63 (link) and standard simulation
settings.108 (link) Additional details are provided
in Supporting Information. All the analyses
were performed on the last 5 μs of each simulation system.
Corradi V., Mendez-Villuendas E., Ingólfsson H.I., Gu R.X., Siuda I., Melo M.N., Moussatova A., DeGagné L.J., Sejdiu B.I., Singh G., Wassenaar T.A., Delgado Magnero K., Marrink S.J, & Tieleman D.P. (2018). Lipid–Protein Interactions Are Unique Fingerprints for Membrane Proteins. ACS Central Science, 4(6), 709-717.
Publication 2018
Adenosinetriphosphatase alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid AMPA Receptors AQP1 protein, human Aquaporin 1 Cell Membrane Proteins Ceramides Cholesterol Debility Diacylglycerol Dopamine Transporter Epidermal Growth Factor Receptor Gangliosides Glucose Transporter Glutamate Glutamate Receptor Lipids Lysophosphatidylcholines Na(+)-K(+)-Exchanging ATPase P-Glycoproteins Phosphates Phosphatidic Acid Phosphatidylcholines phosphatidylethanolamine Phosphatidylinositols Phosphatidylserines Potassium Channel Proteins PTGS1 protein, human SLC2A1 protein, human Sphingomyelins Tissue, Membrane Vertebral Column

Most recents protocols related to «Lysophosphatidylcholines»

1
Comprehensive Metabolomic Profiling via LC-MS/MS
The metabolomic profile was assessed with a validated targeted metabolomics approach, implementing liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), and using the AbsoluteIDQ p180 kit (Biocrates Life Sciences AG AbsoluteIDQ® p180 Kit, Innsbruck, Austria), which benefits of an established good interlaboratory reproducibility (27 (link)). Briefly, the serum samples were placed on a 96-well plate pre-loaded with the isotopic labeled internal standards, along with a phosphate buffer solution as blank sample, a calibration curve (7 levels), and three levels of quality control samples. Two different plates were implemented for this study. The sample preparation consisted in the derivatization of amino acids and biogenic amines with phenyl isothiocyanate, evaporation, extraction with 5 mM ammonium acetate in methanol, centrifugation, and dilution. Amino acids and biogenic amines were separated and analyzed through an analytical column before the mass spectrometry (LC-MS/MS), while lipids and the hexose were analyzed with a simple flow injection analysis (FIA-MS/MS). A total of 188 metabolites were measured, including 21 amino acids, 21 biogenic amines, the sum of hexoses, 40 acylcarnitine, 15 sphingolipids (SM), and 90 glycerophospholipids among which 14 lysophosphatidylcholines (LysoPC), 38 diacylphosphatidylcholine (PC aa), and 38 acylalkylphosphatidylcholine (PC ae). Further instrumental and analytical details have been previously reported (28 (link)).
Borroni E., Frigerio G., Polledri E., Mercadante R., Maggioni C., Fedrizzi L., Pesatori A.C., Fustinoni S, & Carugno M. (2023). Metabolomic profiles in night shift workers: A cross-sectional study on hospital female nurses. Frontiers in Public Health, 11, 1082074.
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Publication 2023
acylcarnitine Amino Acids ammonium acetate Biogenic Amines Buffers Centrifugation Flow Injection Analysis Glycerophospholipids Hexoses Isotopes Lipids Liquid Chromatography Lysophosphatidylcholines Mass Spectrometry Methanol phenylisothiocyanate Phosphates Serum Sphingolipids Tandem Mass Spectrometry Technique, Dilution
2
Urine and Kidney Lipid Profiling
Urine was collected from nonobese and obese mice and centrifuged at 1,000g for 10 minutes to remove cellular debris. Lysosome-enriched subcellular fractions were isolated from kidneys using a modified version of a method described previously (66 (link)). Kidneys were homogenized with pestles in 1 mL of subcellular fractionation buffer (HEPES 20 mM, sucrose 250 mM, KCl 10 mM, MgCl2 1.5 mM, EDTA 1 mM, EGTA 1 mM, dithiothreitol 8 mM, pH adjusted to 7.5 with NaOH). Debris and nuclei were pelleted at 750g for 12 minutes. The supernatant was centrifuged at 10,000g for 35 minutes to pellet the lysosome-enriched fraction. The pellet was washed once with subcellular fractionation buffer. Lipid extraction from urine and the lysosome-enriched fraction was performed using the Bligh and Dyer method with minor modifications (67 (link)). BMP, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, lysophosphatidylcholine, lysophosphatidylethanolamine, monoacylglycerol, diacylglycerol, triacylglycerol, cholesterol, ceramide, hexose ceramide, lactosylceramide, and sphingomyelin were analyzed by supercritical fluid chromatography (SFC) (Nexera UC system, Shimadzu; equipped with an ACQUITY UPC2 Torus diethylamine [DEA] column: 3.0 mm inner diameter [i.d.] × 100 mm, 1.7 μm particle size, Waters) and triple quadrupole mass spectrometry (TQMS; LCMS-8060, Shimadzu) (DEA-SFC/MS/MS) in multiple reaction monitoring (MRM) mode (68 (link)). Fatty acids and cholesterylester were analyzed using an SFC (Shimadzu) with an ACQUITY UPC2 HSS C18 SB column (3.0 mm i.d. × 100 mm, 1.8 μm particle size, Waters) coupled with a TQMS (Shimadzu) (C18-SFC/MS/MS) in MRM mode (69 (link)). The amount of each lipid species was normalized either to the urine creatinine concentration, measured using a QuantiChrom Creatinine Assay Kit (DICT-500) (BioAssay Systems), or to kidney weight.
Nakamura J., Yamamoto T., Takabatake Y., Namba-Hamano T., Minami S., Takahashi A., Matsuda J., Sakai S., Yonishi H., Maeda S., Matsui S., Matsui I., Hamano T., Takahashi M., Goto M., Izumi Y., Bamba T., Sasai M., Yamamoto M., Matsusaka T., Niimura F., Yanagita M., Nakamura S., Yoshimori T., Ballabio A, & Isaka Y. (2023). TFEB-mediated lysosomal exocytosis alleviates high-fat diet–induced lipotoxicity in the kidney. JCI Insight, 8(4), e162498.
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Publication 2023
Biological Assay Buffers CDw17 antigen Cell Nucleus Cells Ceramides Cholesterol Chromatography, Supercritical Fluid Creatinine Diacylglycerol diethylamine Dithiothreitol Edetic Acid Egtazic Acid Fatty Acids HEPES Hexoses Kidney Laser Capture Microdissection Lipids Lysophosphatidylcholines lysophosphatidylethanolamine Lysosomes Magnesium Chloride Mass Spectrometry Mice, Obese Monoglycerides Phosphatidylcholines Phosphatidylethanolamines Phosphatidylglycerols Phosphatidylinositols Phosphatidylserines Radiotherapy Dose Fractionations Sphingomyelins Subcellular Fractions Sucrose Tandem Mass Spectrometry Triglycerides Urine
3
Comparative Lipid Profiling by FIA-FTMS
The analysis of lipids was performed by direct flow injection analysis (FIA) using a high-resolution Fourier Transform (FT) hybrid quadrupole-Orbitrap mass spectrometer (FIA-FTMS) [53 (link)]. TG, diglycerides (DG) and cholesteryl esters (CE) were recorded in positive ion mode as [M + NH4]+ in m/z range 500–1000 and a target resolution of 140,000 (at m/z 200). CE species were corrected for their species-specific response [54 (link)]. Ceramides (Cer), phosphatidylcholines (PC), ether PC (PC O), phosphatidylethanolamines (PE), ether PE (PE O), phosphatidylglycerols (PG), phosphatidylinositols (PI), and sphingomyelins (SM) were analyzed in negative ion mode in m/z range 520–960; lysophosphatidylcholines (LPC) and lysophosphatidylethanolamine (LPE) in m/z range 400–650. Multiplexed acquisition (MSX) was applied for free cholesterol (FC) and the internal standard FC[D7] [54 (link)]. Lipid annotation is based on the latest update of the shorthand notation [55 (link)].
The datasets from liver and plasma lipidomes were subjected to principal component analysis (PCA) using the MetaboAnalystR 3.2 package for R version 4.2.1. For the PCA, the relative metabolite composition of individual lipid species within the different lipid classes were used. Prior to the PCA, variables with missing values were either excluded from the analyzes if more than 50% of the samples were missing or the missing values were replaced by the limit of detection (1/5 of the minimum positive value of each variable). After normalization by log transformation and autoscaling the remaining values were used for the PCA.
Schäfer L., Grundmann S.M., Maheshwari G., Höring M., Liebisch G., Most E., Eder K, & Ringseis R. (2023). Effect of replacement of soybean oil by Hermetia illucens fat on performance, digestibility, cecal microbiome, liver transcriptome and liver and plasma lipidomes of broilers. Journal of Animal Science and Biotechnology, 14, 20.
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Publication 2023
Ceramides Cholesterol Cholesterol Esters Diglycerides Ethyl Ether Flow Injection Analysis Hybrids Lipidome Lipids Liver Lysophosphatidylcholines lysophosphatidylethanolamine M-200 Phosphatidylcholines Phosphatidylethanolamines Phosphatidylglycerols Phosphatidylinositols Plasma Sphingomyelins
4
Lysolecithin-Induced Demyelination Model
Lysolecithin (LPC)-mediated demyelination was performed as described in Kosaraju et al. (2020) (link). Briefly, 1-month-old WT mice were injected with LPC (Sigma, L1381) (1 μL of 1% solution in 1x PBS) using a stereotactic apparatus at two sites: +1.0 mm AP, +1.0 mm ML, −2.2 mm DV and +0.7 mm AP, +1.0ML, −2.2 DV. Animals were allowed to recover for 14 days.
de Almeida M.M., Watson A.E., Bibi S., Dittmann N.L., Goodkey K., Sharafodinzadeh P., Galleguillos D., Nakhaei-Nejad M., Kosaraju J., Steinberg N., Wang B.S., Footz T., Giuliani F., Wang J., Sipione S., Edgar J.M, & Voronova A. (2023). Fractalkine enhances oligodendrocyte regeneration and remyelination in a demyelination mouse model. Stem Cell Reports, 18(2), 519-533.
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Publication 2023
Animals Demyelination Lysophosphatidylcholines Mice, House
5
Newborn Screening of 57 Metabolites by FIA-MS/MS
The neonatal screening center of G. d’Annunzio University participates in an accredited national network of laboratories that adopts a uniform screening panel covering 57 clinically-relevant metabolites, including 14 amino acids, 2 nucleosides, free carnitine, 35 acyl-carnitines, 4 lysophosphatidylcholines and succinylacetone. Details of the metabolites evaluated and their reference values, deriving from the NeoBase™ 2 Non-derivatized MSMS kit (PerkinElmer Life and Analytical Sciences, Turku, Finland) are reported in Table S1. Metabolites were extracted from plasma specimens using a modified protocol based on the manufacturer’s workflow designed for newborn screening. Briefly, 10 µL of plasma were subjected to protein precipitation by incubation with 125 µL of internal standards (IS) solution (PerkinElmer) for 30 min at 45 °C, 700 rpm (Eppendorf ThermoMixer® C). Proteins were removed after centrifugation (at max speed in an Eppendorf 5424) and clear supernatants (125 µL) were transferred into new vials. An additional 1 h incubation step was required to derivatize succinylacetone (SA). Finally, 100 µL of centrifuged samples were transferred to 96-well plates for injection of 10 µL into the ion source. Acquisition, 1.2 min long injection-to-injection, was carried out on a FIA platform RenataDX Screening System, including a 3777 C IVD Sample Manager and an ACQUITY™ UPLC™ I-Class IVD Binary Solvent Manager, coupled to a Xevo™ TQD IVD tandem quadrupole mass spectrometer (both from Waters Corporation, Milford, MA, USA). The flow gradient for the mobile phase provided by the kit was set as follows: 0.15 mL/min from 0 to 0.170 min; 0.01 mL/min from 0.170 to 0.980 min; 0.7 mL/min from 0.980 to 1.180 min; 0.15 mL/min from 1.180 min to the end. Data were processed by MassLynx™ (IVD) Software V4.2 with NeoLynx™ Application Manager (Waters Corp). Mass spectrometry (MS) parameters and a complete list of the metabolites and their internal standards (ISs) are provided in Table S3. Metabolite extraction from dried venous or capillary blood spotted on filter paper was performed according to PerkinElmer’s protocol using the NeoBase™ 2 non-derivatized MSMS kit. As already described [62 (link)–65 (link)], samples were punched out into 3.2 mm disks for the extraction of amino acids, nucleosides, free carnitine, acyl-carnitines, lysophosphatidylcholines and succinylacetone. Finally, 10 µL of supernatant were analyzed on the same FIA-MS/MS platform described above, using the same parameters for injection, MS acquisition and raw data processing. The micromolar (µM) concentrations of all tested metabolites are presented in Table S7.
De Fabritiis S., Valentinuzzi S., Piras G., Cicalini I., Pieragostino D., Pagotto S., Perconti S., Zucchelli M., Schena A., Taschin E., Berteşteanu G.S., Esposito D.L., Stigliano A., De Laurenzi V., Schiavi F., Sanna M., Del Boccio P., Verginelli F, & Mariani-Costantini R. (2023). Targeted metabolomics detects a putatively diagnostic signature in plasma and dried blood spots from head and neck paraganglioma patients. Oncogenesis, 12(1), 10.
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Publication 2023
acylcarnitine Amino Acids BLOOD Capillaries Centrifugation Levocarnitine Lysophosphatidylcholines Mass Spectrometry Nucleosides Plasma Proteins Solvents succinylacetone Tandem Mass Spectrometry Veins

Top products related to «Lysophosphatidylcholines»

1
Lysolecithin by Merck Group
Sourced in United States
Lysolecithin is a type of phospholipid molecule used in various laboratory applications. It is derived from lecithin, a common component of cell membranes. Lysolecithin is primarily used as a tool for studying membrane structure and function in biological research.
2
Absoluteidq p180 kit by Biocrates
Sourced in Austria, United States, Japan, Germany
The AbsoluteIDQ p180 kit is a targeted metabolomics assay developed by Biocrates. The kit provides a quantitative analysis of up to 188 metabolites from various chemical classes, including acylcarnitines, amino acids, biogenic amines, and lipids. The kit utilizes flow injection analysis-tandem mass spectrometry (FIA-MS/MS) technology to enable the simultaneous measurement of these metabolites in a single analysis.
3
Lysophosphatidylcholine by Merck Group
Sourced in United States
Lysophosphatidylcholine is a lipid molecule that is a component of cell membranes. It is used in various laboratory applications as a research tool.
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L α lysophosphatidylcholine by Merck Group
Sourced in United States
L-α-lysophosphatidylcholine is a lipid molecule used in various laboratory applications. It is a type of phospholipid that can be utilized as a reagent or component in experimental procedures. The core function of this product is to serve as a laboratory tool, without further interpretation of its intended use.
5
Lysophosphatidylcholine by Avanti Polar Lipids
Sourced in United States
Lysophosphatidylcholine is a lipid compound that is a by-product of the enzymatic cleavage of phosphatidylcholine. It is commonly used as a component in various cell culture media and as a tool in biochemical research.
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Phosphatidylethanolamine by Avanti Polar Lipids
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Phosphatidylethanolamine is a lab equipment product manufactured by Avanti Polar Lipids. It is a type of phospholipid, a key component of biological membranes. Phosphatidylethanolamine plays a role in various cellular processes and functions.
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Phosphatidylcholine by Avanti Polar Lipids
Sourced in United States
Phosphatidylcholine is a naturally occurring phospholipid that is a major component of cell membranes. It is a colorless, viscous liquid at room temperature. Phosphatidylcholine is a key structural element in biological membranes and plays a crucial role in cellular function and integrity.
8
Methanol by Thermo Fisher Scientific
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Methanol is a colorless, volatile, and flammable liquid chemical compound. It is commonly used as a solvent, fuel, and feedstock in various industrial processes.
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Phosphatidylserine by Avanti Polar Lipids
Sourced in United States
Phosphatidylserine is a phospholipid found naturally in the human body. It is a key component of cell membranes and plays a role in various cellular processes.
10
Formic acid by Merck Group
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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.

More about "Lysophosphatidylcholines"

Lysophosphatidylcholines (LPCs), also known as lysolecithins, are a class of lipid molecules that play crucial roles in cellular signaling, membrane structure, and metabolic processes.
These amphipathic compounds are derived from phosphatidylcholines (PCs) through the enzymatic removal of one fatty acid, resulting in a single fatty acid chain attached to a glycerophosphocholine backbone.
LPCs are involved in a wide range of biological functions, including inflammation, cell proliferation, and lipid homeostasis.
They serve as important intermediates in the biosynthesis and degradation of phospholipids and can act as second messengers, influencing cell behavior and physiological responses.
The AbsoluteIDQ p180 kit is a targeted metabolomics assay that can quantify LPCs and other lipid species.
Accurate and reproducible protocols for the study of LPCs are essential for advancing our understanding of their roles in health and disease.
PubCompare.ai helps researchers optimize their LPC research by identifying the most reliable and efficient protocols from the scientific literature, preprints, and patents, enhancing protocol selection and product identification to ensure reliable and efficieint studies.
LPCs are closely related to other phospholipids, such as phosphatidylethanolamine (PE) and phosphatidylserine (PS), which are also involved in cellular processes.
The analysis of LPCs often involves the use of methanol and formic acid for sample preparation and separation techniques like liquid chromatography-mass spectrometry (LC-MS).
Understanding the interactions and dynamics of LPCs with these related lipids can provide valuable insights into their roles in health and disease.