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Maltotriose

Maltotriose is a trisaccharide composed of three glucose units linked by α-(1->4) glycosidic bonds.
It is a component of starch and can be produced by the enzymatic hydrolysis of starch or maltose.
Maltotriose has applications in food, pharmaceutical, and industrial processes, and is an important target for research and development.
PubCompare.ai's AI-driven protocol comparison can help unlock the power of maltotriose research by discover8ng the best protocols and products from the literature, pre-prints, and patents with ease.

Most cited protocols related to «Maltotriose»

1
Glycan Array Profiling of Trypanosoma Protein Interactions
Cited 12 times
Glycan arrays consisting of 367 diverse glycans with and without the presence of one of three spacers (sp2, sp3 or sp4 [49 (link)]) were prepared from two previously described glycan libraries [83 (link),84 (link)]. Amine containing glycans with spacer’s sp2, sp3 or sp4 were synthesised as previously described [49 (link)] and glycans without spacers were amine functionalised as previously published [85 (link)]. All glycans were suspended in 1:1 DMF:DMSO at a concentration of 500 mM and were printed onto SuperEpoxy 2 glass slides (ArrayIt, Sunnyvale, CA) using a ArrayIt SpotBot Extreme array spotter in a six pin subarray print per glass slide format. All glycans were printed in replicates of four, including four FITC control spots as well as additional position controls (S1 Fig), per subarray using SMP4 pins and a contact time of 1 second at 60% relative humidity, with pins being reloaded after every 12 spots.
Prior to performing glycan array experiments, slides were scanned using a ProScanArray Microarray 4-laser scanner (Perkin Elmer, Waltham, MA) using the blue argon 488 laser set to the FITC settings (492 nm excitation and 517 nm emission). Array slides were blocked with 0.1% BSA in 50 mM phosphate buffered saline (PBS), pH 7.4 for 5 min at 22°C. After washing with PBS, each slide was dried by placing them in an empty 50 mL tube and centrifuging for 5 min at 200 x g (900 rpm). Recombinant TconTS-LD (2 μg) was incubated at a molar ratio of 1:2:3 with anti His-tag mouse polyclonal antibody (10 mg/mL, Cell Signalling Technology), anti mouse-IgG-Alexa555 conjugated rabbit polyclonal antibody (2 mg/mL, Life Technologies) and anti rabbit-IgG-Alexa555 conjugated goat polyclonal antibody (2 mg/mL, Life Technologies) in 50 mM PBS, pH 7.4 containing 0.1% BSA and 10 mM maltotriose for 15 min on ice protected from light. All subarrays on the slide were isolated using a Gene Frame (1.5 x 1.6 cm, 65 μL, Abgene, Epsom, UK) prior to the addition of the TconTS-LD-antibody mix to the array. A coverslip was applied to the GeneFrame and array slides incubated for 30 min at 22°C in the dark. The GeneFrame and coverslip were subsequently removed and the slide gently washed twice with 50 mM PBS, pH 7.4 containing 0.01% TWEEN 20 and 10 mM maltose, and once with 50 mM PBS, pH 7.4 containing 10 mM maltose. Slides were dried by centrifugation for 5 min at 200 x g (900 rpm), allowed to air dried for a further 5 min, and the fluorescence associated with the array spots detected using the microarray scanner settings outlined above. Image analysis and spot visualisation was performed using the ProScanArray software, ScanArray Express (Perkin Elmer). The resulting images were visually examined. Fluorescence signals were judged as being positive, if all four replicates for a glycan were clearly detectable (S1 Fig).
Waespy M., Gbem T.T., Elenschneider L., Jeck A.P., Day C.J., Hartley-Tassell L., Bovin N., Tiralongo J., Haselhorst T, & Kelm S. (2015). Carbohydrate Recognition Specificity of Trans-sialidase Lectin Domain from Trypanosoma congolense. PLoS Neglected Tropical Diseases, 9(10), e0004120.
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Publication 2015
2
Quantitative Glycomic Analysis of HeLa Cells
Cited 4 times
HeLa cells transfected with control or GRASP RNAi were washed with cold PBS, scraped and collected by centrifugation. Proteins were harvested from the cell pellets as previously described53 (link). Briefly, the cell pellets were resuspended in ice-cold water and disrupted by Dounce homogenization on ice. The homogenate was adjusted to 4:8:3::choloroform:methanol:water before removal from ice. After 18 hours of end-over-end agitation (Nutator), the insoluble proteinacious material was collected by centrifugation. Following re-extraction of the pellet three times with 4:8:3 and once with ice-cold 80% acetone (v/v, aqueous) the final pellet was dried under a gentle stream of nitrogen and stored as a fine white powder. All supernatants were combined and dried under nitrogen for analysis of LLO and FOS. Protein content of the powder was determined by BCA assay (Pierce). N-linked glycans were harvested from the protein powder as previously described53 (link). Generally, 2 mg of protein powder (dry weight) was resuspended and digested with trypsin/chymotrypsin. Glycopeptides were enriched by Sep-Pak C18 cartridge chromatography and subjected to digestion with PNGase F for 18 h at 37°C. Released oligosaccharides were separated from residual peptide by Sep-Pak C18 clean-up and permethylated prior to mass spectrometric analysis54 .
For analysis of LLO and FOS, the dried supernatants from the cell extraction were resuspended in 50% aqueous methanol and loaded onto a Sep-Pak C18 column previously washed with methanol and equilibrated in water. FOS were collected in the run-through and a subsequent water wash, which were combined and dried. The collected FOS fraction was permethylated for analysis by MS54 . The LLOs remained bound to the Sep-Pak C18 column and were recovered by elution with methanol and 10:10:3::chloroform:methanol:water as previously described55 (link). Briefly, after drying, the LLO fraction was resuspended in 10:10:3 and applied to a DEAE-cellulose column. Following washes with 10:10:3 to remove neutral lipids, the LLOs were eluted in 10:10:3 containing 300 mM ammonium acetate. The eluted LLOs were recovered by phase partition into the organic layer, which was dried and resuspended in 0.1 M HCl in isopropanol. The LLO oligosaccharides were released from dolichol pyrophosphate by heating at 50°C for 1 h. After cooling and drying under nitrogen, the released LLO oligosaccharides were resuspended in water and recovered in the aqueous phase after addition of n-butanol. The LLO oligosaccharides in the aqueous phase were dried by lyophilization and permethylated for analysis by MS54 .
Permethylated N-linked, FOS, and LLO glycans were analyzed by nanospray ionization mass spectrometry using an ion trap instrument (NSI-LTQ Orbi Discoverer, Thermo-Fisher) by dissolving in 1 mM NaOH in 50% methanol and directly infusing into the instrument at a syringe flow rate of 0.40–0.60 µl/min. For fragmentation by CID in MS/MS and MSn modes 40% collision energy was applied. The total ion mapping (TIM) functionality of the XCalibur software package (version 2.0) was utilized to detect and quantify the prevalence of individual glycans in the total glycan profile. Most permethylated oligosaccharides were identified as singly, doubly, and triply charged species by NSI-MS. TIM peaks for all charge states of a given ion with m/z ≤ 2000 were summed together for quantification. Glycan quantification was performed relative to a known quantity of external standards (maltotriose and maltotetraose, Supelco) that was permethylated with heavy methyliodide (13CH3I) and spiked into the sample matrix.
Xiang Y., Zhang X., Nix D.B., Katoh T., Aoki K., Tiemeyer M, & Wang Y. (2013). Regulation of protein glycosylation and sorting by the Golgi matrix proteins GRASP55/65. Nature communications, 4, 1659.
Publication 2013
Acetone ammonium acetate Biological Assay Butyl Alcohol Centrifugation Chloroform Chromatography chymotrypsin - trypsin Cold Temperature DEAE-Cellulose Digestion dolichol pyrophosphate Freeze Drying Glycopeptidase F Glycopeptides GRASP protein, human HeLa Cells Isopropyl Alcohol Lipids maltotetraose maltotriose Mass Spectrometry Methanol methyl iodide MS 54 Nitrogen Oligosaccharides Pellets, Drug Peptides Polysaccharides Powder Proteins RNA Interference Sep-Pak C18 Syringes Tandem Mass Spectrometry
3
Thin-Layer Chromatography for Maltase Assay
Cited 3 times
To verify substrate hydrolysis and detect the products formed, MAL1 WT and mutant Asp199Ala reaction mixtures were separated on TLC plates (Silica Gel 60 F254) with concentrating zones (Merck, Germany). The reactions were conducted in maltase buffer at 37°C, and at certain time points aliquots were withdrawn and heated for 5 min at 96°C to inactivate the enzyme. Concentrations of 1‐kestose, nystose, melezitose and maltotriose in the reaction mixture were 50 mm, 6‐kestose was used at 10 mm, and malt extract and IMOs were used at 2% w/v. Palatinose and maltose were used at 100 mm in the assay of catalytic ability of the Asp199Ala mutant. Maltase was added at 1 U/ml reaction mixture, or at 247 µg/ml in the case of the Asp199Ala mutant. The amount of protein equivalent to 1 U was determined through activity assays on 1 mmα‐pNPG; 0.5 µl of each of the stopped reaction mixtures were spotted onto TLC plates and sugars were separated with two runs in chloroform:acetic acid:water (6:7:1 v/v/v) (Stingele et al., 1999). Sugars were visualized by immersion of the plates in aniline–diphenylamine reagent and subsequent heating of the dried plates at 120°C (Jork et al., 1990).
Viigand K., Visnapuu T., Mardo K., Aasamets A, & Alamäe T. (2016). Maltase protein of Ogataea (Hansenula) polymorpha is a counterpart to the resurrected ancestor protein ancMALS of yeast maltases and isomaltases. Yeast (Chichester, England), 33(8), 415-432.
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Publication 2016
4-nitrophenylgalactoside 6-kestose Acetic Acid alpha Glucosidase aniline Biological Assay Buffers Catalysis Chloroform Diphenylamine Enzymes Hydrolysis isomaltulose Maltose maltotriose melezitose nystose Proteins Silica Gel Submersion Sugars
4
Characterization of S. suis MsmK Mutant
Cited 3 times
The bacterial strains and plasmids used in this study are listed in Table 1. S. suis strain SC-19 (streptomycin-resistant) of serotype 2 used in this study was isolated from a sick pig during the epidemic outbreak in Sichuan province of China in 2005 [37 (link)]. SC-19 and its genetically modified strains were grown in tryptic soy broth (TSB) or on tryptone soy agar (TSA) (Difco, France) supplemented with 10% fetal bovine serum (FBS) (Sijiqing, Hangzhou, China) at 37°C. For screening mutant strain ΔmsmK, erythromyein (90 μg/ml) was added. For construction of complementary strain CΔmsmK, erythromyein (90 μg/ml) and spectinomycin (100 μg/ml) were added when needed. Chemically defined medium (CDM) [38 (link)] supplemented with no sugar, or 1% of glucose, maltose, maltotriose, maltotetraose, raffinose, melibiose or glycogen as indicated was used for growth assays and Western blot. Bacteria cultured with CDM were grown at 37°C.
E. coli DH5α (Trans, China) was used as a host strain for cloning, and E. coli BL21 (DE3) (Trans) was used as a host strain to express His-tag fusion protein MsmK. Both were cultured in Luria-Bertani (LB) broth (Difco) or plated on LB agar plates at 37°C or 18°C.When appropriate, kanamycin (25 μg/ml) was added for bacterial selection.
Unless otherwise specified, all the antibiotics, chemicals and substrates were purchased from Biosharp (Hefei, China).
Tan M.F., Gao T., Liu W.Q., Zhang C.Y., Yang X., Zhu J.W., Teng M.Y., Li L, & Zhou R. (2015). MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis. PLoS ONE, 10(7), e0130792.
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Publication 2015
Agar Antibiotics, Antitubercular Bacteria Biological Assay Carbohydrates Culture Media Epidemics Escherichia coli Fetal Bovine Serum Glucose Glycogen Kanamycin Maltose maltotetraose maltotriose Melibiose Plasmids Proteins Raffinose Spectinomycin Strains Streptomycin tryptic soy broth Western Blotting
5
Momentary Mood and Salivary Alpha-Amylase
Cited 3 times

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Publication 2011
2-nitrophenol Anger Arousal Biological Assay Emotions Hydrocortisone Kinetics maltotriose Mood Nervousness Plant Roots Saliva Transients

Most recents protocols related to «Maltotriose»

1
Validation of Molecular Docking Setup
Not available on PMC !
Authentication of the molecular docking procedure was performed by redocking the two cocrystallized ligands, MCC950 and α-maltotriose, in the active sites of NLRP3 and the ASC-CARD, respectively. The performed validation reproduced the same binding patterns and interactions of the cocrystallized ligands, confirming that the docking setup used was appropriate for the proposed docking study. This was illustrated by the perfect alignment between the self-docked poses and the cocrystallized ligands MCC950 and α-maltotriose, with root mean square deviations (RMSDs) of 1.262 and 0.52 Å and docking scores (S) of -9.2 and -9.9 kcal mol -1 in the binding sites of NLRP3 and ASC-CARD, respectively.
Ali S.O., Ghaiad H.R., Elmasry G.F, & Mehana N.A. (2024). Sinapic Acid Mitigates Pentylenetetrazol-induced Acute Seizures By Modulating the NLRP3 Inflammasome and Regulating Calcium/calcineurin Signaling: In Vivo and In Silico Approaches. Inflammation.
Publication 2024
2
Determining Exponential Growth Phase via OD600
Not available on PMC !
To determine which OD600 readings corresponded to exponential phase, a 48-hour broth culture (mNYC III supplemented with 1% oyster glycogen or maltotriose) was diluted 1:10 and 200 µL aliquots of the diluted sample were pipetted into 8 wells of a flat bottom 96-well plate (VWR Tissue Culture Plate, Non-treated, Sterilized, Non-Pyrogenic, Avantor). The 96-well plate was then incubated over the course of 72 hours at 37°C under anaerobic conditions (GasPak EZ Anaerobe Gas Generating Pouch System with Indicator, BD) with OD600 readings taken periodically over 72 hours with a microplate reader (Varikoskan LUX 3020-1160). The time spent to read the OD600 was minimized to ensure little disturbance and the GasPak was replaced at each time point. Growth curves were performed for each strain in each condition (maltotriose or glycogen). Data was fitted to a logistic growth model using GraphPad Prism version 10.1.0 for macOS (GraphPad Software, Boston, USA).
Kim A., Fernando C., Kularatne D., & Hill J.E. (2024). Investigation of expression of genes encoding glycogen degrading enzymes inGardnerella swidsinskiiand identification of reference genes for quantitative real-time PCR.
Publication 2024
3
Purified Maltooligosaccharides and Starch Substrates
Not available on PMC !
Maltooligosaccharides (purity ≥ 95%), including maltose (G2), maltotriose (G3), maltotetraose (G4), maltopentaose (G5), and maltohexaose (G6), were purchased from Wako Pure Chemical (Osaka, Japan).
The substrates (soluble starch, wheat starch, corn starch, potato starch and cassava starch) were acquired from the Sigma Chemical Co. (St. Louis, MO, USA).
Wang Y., Xie T., Yan G., Xue H., Zhao Z, & Ye X. (2024). Heterologous Expression and Characterization of a Novel Mesophilic Maltogenic α-Amylase AmyFlA from Flavobacterium sp. NAU1659. Applied biochemistry and biotechnology.
Publication 2024
4
Wort Mashing and Composition Analysis

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Publication 2024
5
Cryo-EM and Crystal Structure Docking
Not available on PMC !
The PDB was used for obtaining the cryo-electron microscopy and crystal structures of the selected targets. Human NLRP3 bound to the specific inhibitor MCC950 (PDB ID: 7VTP) [66] (link) and the crystal structure of the human ASC-CARD complex bound to α-maltotriose (PDB ID: 6KI0) [67] (link) were selected for the subsequent docking study.
Ali S.O., Ghaiad H.R., Elmasry G.F, & Mehana N.A. (2024). Sinapic Acid Mitigates Pentylenetetrazol-induced Acute Seizures By Modulating the NLRP3 Inflammasome and Regulating Calcium/calcineurin Signaling: In Vivo and In Silico Approaches. Inflammation.
Publication 2024

Top products related to «Maltotriose»

1
Maltotriose by Merck Group
Sourced in United States, Sao Tome and Principe
Maltotriose is a trisaccharide composed of three glucose units. It is a key intermediate in the breakdown of starch and glycogen. Maltotriose serves as a substrate for various enzymes involved in carbohydrate metabolism.
2
Maltose by Merck Group
Sourced in United States, Germany, United Kingdom, Sao Tome and Principe, Switzerland, Australia, Italy, China, France, Greece
Maltose is a disaccharide composed of two glucose units linked together. It is commonly used as a standard in various biochemical and analytical laboratory applications to measure the activity or concentration of enzymes, such as amylases, that cleave maltose into glucose units.
3
Sucrose by Merck Group
Sourced in United States, Germany, United Kingdom, France, Switzerland, Sao Tome and Principe, China, Macao, Italy, Poland, Canada, Spain, India, Australia, Belgium, Japan, Sweden, Israel, Denmark, Austria, Singapore, Ireland, Mexico, Greece, Brazil
Sucrose is a disaccharide composed of glucose and fructose. It is commonly used as a laboratory reagent for various applications, serving as a standard reference substance and control material in analytical procedures.
4
D glucose by Merck Group
Sourced in United States, Germany, United Kingdom, China, Australia, France, Italy, Canada, Sao Tome and Principe, Japan, Macao, Israel, Switzerland, Spain, Belgium, India, Poland, Sweden, Denmark, Norway, Ireland, Mexico, New Zealand, Brazil, Singapore, Netherlands
D-glucose is a type of monosaccharide, a simple sugar that serves as the primary source of energy for many organisms. It is a colorless, crystalline solid that is soluble in water and other polar solvents. D-glucose is a naturally occurring compound and is a key component of various biological processes.
5
Soluble starch by Merck Group
Sourced in United States, Germany, Italy, China, India
Soluble starch is a laboratory reagent used as a thickening, stabilizing, and suspending agent in various applications. It is a naturally occurring polysaccharide derived from plants. Soluble starch is a white, odorless, and tasteless powder that dissolves easily in water, forming a translucent, viscous solution.
6
Methanol by Merck Group
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
7
D fructose by Merck Group
Sourced in United States, Germany, Italy, Belgium, China, United Kingdom, Sao Tome and Principe, India
D-fructose is a monosaccharide that can be used as a laboratory reagent. It is a natural sugar found in fruits and honey. D-fructose has the molecular formula C6H12O6 and serves as a fundamental building block for carbohydrates.
8
Maltotetraose by Merck Group
Sourced in United States
Maltotetraose is a carbohydrate compound consisting of four glucose units. It is a common laboratory reagent used in various biochemical and analytical applications.
9
Fructose by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, Australia, China, Sao Tome and Principe, Spain, Switzerland, France, Sweden, Canada, Belgium, Poland, Greece, India
Fructose is a type of monosaccharide sugar that is commonly used in laboratory settings. It is a naturally occurring carbohydrate found in fruits, honey, and certain vegetables. Fructose serves as a key component in various experimental and analytical procedures, particularly in the fields of biochemistry, food science, and nutrition research.
10
Carbopac pa1 column by Thermo Fisher Scientific
Sourced in United States, Germany
The CarboPac PA1 column is a high-performance anion-exchange chromatography column designed for the analysis of carbohydrates. It features a polymer-based packing material that provides excellent resolution and peak shape for a wide range of carbohydrate species.

More about "Maltotriose"

Maltotriose is a trisaccharide composed of three glucose units linked by α-(1->4) glycosidic bonds.
It is a key component of starch and can be produced through the enzymatic hydrolysis of starch or maltose.
This versatile carbohydrate has a wide range of applications in the food, pharmaceutical, and industrial sectors, making it an important target for research and development.
Closely related to maltotriose are other saccharides like maltose, sucrose, and D-glucose.
Maltose is a disaccharide made up of two glucose units, while sucrose is a disaccharide composed of glucose and fructose.
D-glucose, also known as dextrose, is a monosaccharide that serves as a building block for larger carbohydrates like starch and maltodextrins.
Soluble starch, a mixture of amylose and amylopectin, is another important carbohydrate that is closely tied to maltotriose.
Methanol and D-fructose are also relevant compounds, as they can be involved in the production or modification of maltotriose and related substances.
To unlock the full potential of maltotriose research, tools like PubCompare.ai's AI-driven protocol comparison can help scientists discover the best protocols and products from the literature, pre-prints, and patents with ease.
By leveraging intelligent AI, researchers can optimize their work and make more informed decisions, leading to breakthroughs in the field of maltotriose and related carbohydrate chemistry.
Whether you're working with a CarboPac PA1 column or exploring new applications for maltotriose, PubCompare.ai can be a valuable resource in your research endeavors.
With its comprehensive database and advanced analytical capabilities, you can uncover the latest developments and insights in this dynamic area of study.