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Mangostin

Mangostin is a natural compound derived from the mangosteen fruit (Garcinia mangostana), a tropical evergreen tree native to Southeast Asia.
This polyphenol compound has garnered attention for its potential health benefits, including anti-inflammatory, antioxidant, and antimicrobial properties.
Mangostin's diverse biological activities have made it a subject of intensive research in fields such as medicine, nutrition, and pharmacology.
Optimizing your Mangostin research can be facilitated by tools like PubCompare.ai, which utilizes AI-driven technology to help researchers easily locate relevant protocols from literature, preprints, and patents, while identifying the best protocols and products through intelligent comparisons.
This can enhance the reproducibility and accuracy of your Mangostin studies.

Most cited protocols related to «Mangostin»

1
Antiviral Evaluation of α-Mangostin
The antiviral effect of the compounds (at the maximum non-toxic dose) were assessed before infection (pre-treatment of cells), during infection (co-treatment) and after infection (post-treatment). During pre-treatment, the cells (50,000 cells/well) were pre-treated with compound at 37 °C for 24 h. Then the compound containing culture supernatant was removed and the cells were infected with 0.1 MOI of DENV-2. After infection, the cells were washed twice to remove the unbounded virus and kept for incubation by adding maintenance media (MEM with antibiotics and 2% Fetal bovine serum). In co-treatment, the virus was mixed with different concentrations of the compound and the mixture was used for infecting cells for duration of one h. For post-treatment, the cells were infected with 0.1 MOI DENV-2 for 1 h. and treated with the compound after 24 h. For all types of treatments, the cells were incubated for five days post-infection. In the virus control, wells contain only infected cells without any treatment while the cell control contains uninfected cells only. In all conditions, plates were frozen at −80 °C and thawed to collect cell supernatant for quantitative estimation of viral RNA.
For the compound α-Mangostin which showed antiviral activity, the assays were repeated under pre-, co- and post-treatment conditions with different concentrations of the compounds as described earlier. The collected supernatant was used for determination of viral genomic RNA level by real-time RT-PCR and titer of infectious virus particle by focus forming unit assay. All the experiments were performed in triplicates in two independent trials.
Panda K., Alagarasu K., Patil P., Agrawal M., More A., Kumar N.V., Mainkar P.S., Parashar D, & Cherian S. (2021). In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2). Molecules, 26(10), 3016.
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Publication 2021
Antibiotics Antiviral Agents Biological Assay Cells Fetal Bovine Serum Freezing Genome Infection mangostin Real-Time Polymerase Chain Reaction RNA, Viral Viral Genome Virus
2
Caspase-3 Quantification by ELISA
Caspase-3 levels were measured by ELISA kit (Elabsciences, China) in the brain homogenate. The procedure was performed according to the instructions of the manufacturer. The assay employs the enzyme immunoassay competitive method [67 (link)].
Tiwari A., Khera R., Rahi S., Mehan S., Makeen H.A., Khormi Y.H., Rehman M.U, & Khan A. (2021). Neuroprotective Effect of α-Mangostin in Ameliorating Propionic Acid-Induced Experimental Model of Autism in Wistar Rats. Brain Sciences, 11(3), 288.
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Publication 2021
Biological Assay Brain Caspase 3 Enzyme-Linked Immunosorbent Assay Enzyme Immunoassay Enzymes Immunoassay
3
Cytotoxicity Screening of Apigenin, α-Mangostin, and Doxorubicin
CCD-986Sk and WI-38 cells were seeded at 1 × 104 cells/well in 96-well plates containing 200 μL of medium for overnight culture, whereas SKOV-3 cells were cultured in the same manner but seeded at 5 × 103 cells/well. Then, the cells were treated with various concentrations of apigenin, α-mangostin, or doxorubicin, or the 0.1% (v/v) dimethyl sulfoxide (DMSO) solvent only (control). The SKOV-3 cells were treated for 24, 48, and 72 hr, whereas the CCD-986Sk and WI-38 cells were treated for 24 hr only. After the indicated incubation (exposure) time was reached, 10 μL of 5 mg/mL MTT solution was added to each well and the culture plates were incubated for 3 hr to allow for mazan formation. The culture medium was then removed, the formazan was solubilized by the addition of 150 μL of DMSO, and the absorbance at 560 nm (A560) was measured with a microplate reader. The cell viability (%) was calculated using Eq. (1) as follows:
The IC50 value of each compound was calculated from the graphical plot of the relative number of viable cells (%) vs. the test compound concentration.
Ittiudomrak T., Puthong S., Roytrakul S, & Chanchao C. (2019). α-Mangostin and Apigenin Induced Cell Cycle Arrest and Programmed Cell Death in SKOV-3 Ovarian Cancer Cells. Toxicological Research, 35(2), 167-179.
Publication 2019
Apigenin Cells Cell Survival Culture Media Doxorubicin Formazans mangostin Solvents Sulfoxide, Dimethyl
4
Mangiferin Alleviates Arthritis in Rats
Male Sprague Dawley rats (180 ± 10 g) were supplied by Qinglongshan Laboratory Animal Company (Nanjing, Jiangsu, China). The animals were housed in a specific pathogen free (SPF) laboratory with standard environmental conditions. All the rats were fed with sterile rodent chow and water ad libitum. After 7 days acclimatization, AIA was induced in all the rats except for normal healthy controls. Prior to this procedure, heating-inactivated BCG was carefully grinded in IFA, and subsequently homogenized with the same volume of water to obtain complete Freund’s adjuvant (CFA, 20 mg/ml). On day 0, an intradermal injection of 0.1 ml CFA was carried out on the right hind paw, which was followed by a boost CFA injection at the base of tail 7 days later. Thereafter, the rats were divided into three groups with six rats each, and received different oral treatments:
Group 1: Normal healthy control (normal) administered with 0.5% sodium carboxymethyl cellulose solution (CMC-Na); Group 2: AIA model control (AIA) administered with 0.5% CMC-Na; Group 3: MAN-treated AIA rats (MAN + AIA) administered with MAN (dispersed in 0.5% CMC-Na by the aid of ethanol and tween-80).
The therapeutic dose of MAN was designated as 50 mg/kg, as it can result in effective concentrations in both blood and tissues (Xu et al., 2017 (link)). Also, MAN at this dose can effectively cure experimental arthritis (Yang et al., 2019 (link)). The treatment duration was 30 days, and body weight of all the rats was periodically recorded. All the animal experiment procedures were performed in accordance with the national institutes of health guide for the care and use of laboratory animals (NIH Publications No. 8023, revised 1978) and the approval for animal studies in this study was obtained from the Ethics Committee of Wannan Medical College.
Hu Y.H., Han J., Wang L., Shi C., Li Y., Olatunji O.J., Wang X, & Zuo J. (2021). α-Mangostin Alleviated Inflammation in Rats With Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback. Frontiers in Pharmacology, 12, 692806.
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Publication 2021
Acclimatization Administration, Oral Animals Animals, Laboratory Arthritis, Experimental BLOOD Body Weight Ethanol Ethics Committees Freund's Adjuvant Intradermal Injection Rats, Sprague-Dawley Rattus norvegicus Rodent Sodium Carboxymethylcellulose Specific Pathogen Free Sterility, Reproductive Tail Therapeutics Tissues Tween 80
5
GMCT: Standardized Herbal Blend Extract
GMCT (LI80020F4 or CinDura®, Laila Nutraceuticals, Vijayawada, India) is a proprietary composition; it includes seven parts of an herbal blend containing aqueous ethanol extracts of Garcinia mangostana (GM) fruit rind and Cinnamomum tamala (CT) leaf at 1:2 ratio and three parts of the excipients. GMCT contains 28.1% (w/w) microcrystalline cellulose and 1.9% (w/w) Syloid as excipients. The final product was standardized to contain at least 3.5% α-mangostin and 0.1% rutin.
Konda M.R., Alluri K.V., Janardhanan P.K., Trimurtulu G, & Sengupta K. (2018). Combined extracts of Garcinia mangostana fruit rind and Cinnamomum tamala leaf supplementation enhances muscle strength and endurance in resistance trained males. Journal of the International Society of Sports Nutrition, 15, 50.
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Publication 2018
Cinnamomum Ethanol Excipients Fruit Garcinia mangostana mangostin microcrystalline cellulose Nutraceuticals Plant Leaves Rutin

Most recents protocols related to «Mangostin»

1
Computational Analysis of Alpha-Mangostin's ADME and Biological Activities
Not available on PMC !
The assessment of the ADME & Toxicity (Absorption, Distribution, Metabolism, Excretion, and Toxicity) characteristics of the selected alpha-mangostin molecule was conducted through the utilization of the pkCSM online tool. The SMILE ID corresponding to alpha-mangostin was retrieved from the PubChem database (https://pub-chem.ncbi.nlm.nih.gov/#query=alpha-Mangostin) and subsequently employed in the pkCSM server to elucidate its ADME and Toxicity properties.
In the realm of chemoinformatics and drug discovery, the Prediction of Activity Spectra for Substances (PASS) serves as a computational instrument for forecasting the biological activities inherent to chemical compounds. For the execution of the PASS analysis, the chemical structure of alpha-mangostin, delineated in the standardized "SMILES" format, was submitted to the online server accessible at http://www.pharmaexpert.ru/passonline/ to ascertain the likelihood of biological activities associated with this compound.
Furthermore, to explore the potential associations of alpha-mangostin with various biological properties, the SwissTargetPrediction online tool, accessible via http:// www.swisstargetprediction.ch/, was also employed in this investigation.
Razique A. (2024). Identification of small molecule inhibitors of penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus for the therapeutics of bacterial infection. Cellular and molecular biology (Noisy-le-Grand, France), 70(3).
Publication 2024
2
Synthesis and Characterization of Bioactive Compound
Not available on PMC !
Dark powder. Yield 53.74 %; m.p. 18 8-190°C. UV (MeOH) λmax: 217 nm. 1 HNMR (DMSO, 500 MHz) δ 6.62 (s, 1H, H-4), δ 6.75 (s, 1H, H-5), δ 3.30 (t, 1H, H-8a), δ 4.12 (d, 1H, J = 6.5 Hz, H-9), δ 3.95 (t, 1H, H-10a), δ 3.94 (d, 2H, H-11), δ 5.09 (m, 1H, H-12), δ 1.114 (s, 3H, H-14), δ 1.07 (s, 3H, H-15), δ 3.94 (d, 2H, J = 6.5 Hz, H-16), δ 5.24 (t, 1H, H-17), δ 1.14 (s, 3H, H-19), δ 1.07 (s, 3H, H-20). 13
Mardianingrum R., Endah S.R.N., Daruwati I., Muchtaridi M., & Ruswanto R. (2024). Synthesis and computational study of metal complex of α-mangostin as an anticancer candidate. Journal of Pharmacy & Pharmacognosy Research, 12(3), 423-438.
Publication 2024
3
Isolation and Characterization of Bioactive Compound
Not available on PMC !
). Greenishyellow powder. Yield 89.46%; m.p. 18 4-186°C. UV (MeOH) λmax: 244 nm (I) and 261 nm (II). 1 HNMR (DMSO, 500 MHz) δ 6.74 (s, 1H, H-4), δ 6.80 (s, 1H, H-5), δ 3.15 (t, 1H, H-8a), δ 3.98 (d, 1H, J = 6.5 Hz, H-9), δ 3.96 (d, 2H, H-11), δ 5.11 (m, 1H, H-12) , δ 1.62 (s, 3H, , H-14), δ 1.83 (s, 3H, H-15), δ 4.08 (d, 2H, J = 6.5 Hz, H-16), δ 5.13 (m, 1H, H-17), δ 1.69 (s, 3H, H-19), δ 1.58 (s, 3H, H-20), δ 3.65 (s, 3H, OCH3). 13
Mardianingrum R., Endah S.R.N., Daruwati I., Muchtaridi M., & Ruswanto R. (2024). Synthesis and computational study of metal complex of α-mangostin as an anticancer candidate. Journal of Pharmacy & Pharmacognosy Research, 12(3), 423-438.
Publication 2024
4
Quantitative Analysis of α-Mangostin in Extracts
Not available on PMC !
For the quantitative analysis of α-mangostin content in the extract, we adapted the HPLC method outlined by Bundeesomchok et al. (2016) (link) with modifications. Our method utilized a binary HPLC pump (Shimadzu 33236, Tokyo, Japan), coupled with a photodiode array detector (Shimadzu 12623, Tokyo, Japan) and an autosampler (Shimadzu 03224, Tokyo, Japan). Furthermore, we employed a 150 × 4.6 mm ACE 5 C18-PFP column (Advanced Chromatography Technologies, Aberdeen, Scotland). Standardization of the sample injection volume was set at 20 µL, and the column was subjected to isocratic elution using a blend of acetonitrile and 0.2% formic acid (70:30, v/v) at a flow rate of 1 mL/min. The concentration of α-mangostin was determined at a wavelength of 240 nm, utilizing a calibration curve constructed from five α-mangostin concentrations (ranging from 12.5 to 200 µg/mL), with their respective peak areas plotted against concentrations. Each calibration concentration underwent triplicate analysis to establish the linear equation Y = 93,150X -32,893 (R 2 = 1). The analysis revealed that the α-mangostin extract contained 0.04% (w/v) α-mangostin (Figure 1).
Meah M.S., Panichayupakaranant P., Silvano K.J., Ugli R.A.R., & Kim M.G. (2024). A Comparative Study on Antibacterial Activities of Alcohol Disinfectants and α-Mangostin Extract. Pharmacognosy Magazine.
Publication 2024
5
Microwave-Assisted Extraction of Mangosteen Pericarp
Not available on PMC !
Dried powders of G. mangostana pericarps (1.5 kg) were subjected to microwave-assisted extraction using a microwave frequency of 1,450 MHz and an electric power of 900 W. The extraction process comprised three cycles, each consisting of 8 minutes of power-on followed by 1 minute of power-off, conducted at 90°C. During the extraction, PEG 400 (3 L) was utilized as the solvent.
Meah M.S., Panichayupakaranant P., Silvano K.J., Ugli R.A.R., & Kim M.G. (2024). A Comparative Study on Antibacterial Activities of Alcohol Disinfectants and α-Mangostin Extract. Pharmacognosy Magazine.
Publication 2024

Top products related to «Mangostin»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
α mangostin by Merck Group
Sourced in United States
α-mangostin is a laboratory reagent used as a reference standard. It is a naturally occurring xanthone compound isolated from the pericarp of the mangosteen fruit (Garcinia mangostana).
3
Dimethyl sulfoxide (dmso) by Merck Group
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
4
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
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GraphPad Prism 5 is a data analysis and graphing software. It provides tools for data organization, statistical analysis, and visual representation of results.
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Methyl thiazolyl tetrazolium (mtt) by Merck Group
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MTT is a colorimetric assay used to measure cell metabolic activity. It is a lab equipment product developed by Merck Group. MTT is a tetrazolium dye that is reduced by metabolically active cells, producing a colored formazan product that can be quantified spectrophotometrically.
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Specord 200 by Analytik Jena
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The Specord 200 is a UV-Vis spectrophotometer designed for accurate and reliable absorbance measurements. It features a double-beam optical system and a wavelength range of 190 to 1100 nanometers. The Specord 200 provides precise and reproducible results for a variety of analytical applications.
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Prism 6 by GraphPad
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Prism 6 is a data analysis and graphing software developed by GraphPad. It provides tools for curve fitting, statistical analysis, and data visualization.

More about "Mangostin"

Mangosteen (Garcinia mangostana) is a tropical evergreen tree native to Southeast Asia, and its fruit is the source of the natural compound mangostin.
This polyphenol has garnered significant attention for its potential health benefits, including anti-inflammatory, antioxidant, and antimicrobial properties.
Mangostin's diverse biological activities have made it a subject of intensive research in various fields such as medicine, nutrition, and pharmacology.
Optimizing your mangostin research can be facilitated by tools like PubCompare.ai, which utilizes AI-driven technology to help researchers easily locate relevant protocols from literature, preprints, and patents.
This platform can also identify the best protocols and products through intelligent comparisons, enhancing the reproducibility and accuracy of your mangostin studies.
Mangostin research often involves the use of related compounds and materials, such as α-mangostin, a structural isomer of mangostin, and DMSO, a commonly used solvent.
Additionally, cell culture techniques may utilize FBS (fetal bovine serum), Penicillin/streptomycin, and TRIzol reagent for RNA extraction.
Analytical tools like GraphPad Prism 5, Prism 8, and Specord 200 can be employed for data analysis and visualization.
By leveraging the capabilities of PubCompare.ai and incorporating the use of relevant materials and analytical tools, researchers can enhance the efficiency, reproducibility, and accuracy of their mangostin-related studies, ultimately contributing to the advancement of knowledge in this exciting field of research.