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Mannitol

Mannitol is a naturally occurring sugar alcohol found in various plants and fungi.
It has diverse applications in the pharmaceutical, food, and cosmetic industries.
Mannitol is commonly used as a diuretic, laxative, and osmotic agent in the treatment of various medical conditions, including increased intracranial pressure, glaucoma, and cerebral edema.
It also serves as a bulking agent and sweetener in the formulation of medications, food products, and personal care items.
Mannitol is known for its low caloric value and ability to maintain moisture, making it a popular choice in suger-free and moisture-retaining formulations.
Reseach on the efficacy and reproducibility of mannitol-based products is crucial for optimizing its clinical and commercial utilization.
PubCompare.ai provides an AI-driven platform to quickly locate and compare mannitol research protocols from literature, preprints, and patents, enhancing the accuracy and reproducibilty of mannitol experiments.

Most cited protocols related to «Mannitol»

1
Efficient Arabidopsis Protoplast Isolation
Leaves (width: 2 cm, length: 5 cm in optimal light condition; width: 0.5 cm; length: 2.5 cm in low light conditions) were collected from 3 to 5-week-old plants grown under optimal light (ca. 150 μE·m-2·s-1) or low light (ca. 50·μE m-2·s-1) conditions. Arabidopsis protoplasts were isolated in two ways. First, to recreate the current technique, protoplasts were made according to the procedure of Yoo et al. [4 (link)]. Second, in a new technique, selected leaves were used in a 'Tape-Arabidopsis Sandwich' experiment. The upper epidermal surface was stabilized by affixing a strip of Time tape (Time Med, Burr Ridge, IL) while the lower epidermal surface was affixed to a strip of Magic tape (3 M, St. Paul, MN). The Magic tape was then carefully pulled away from the Time tape, peeling away the lower epidermal surface cell layer. The peeled leaves (7 to 10 optimal-light-growth leaves, about 1-2 g, up to 5 g), still adhering to the Time tape, were transferred to a Petri dish containing 10 mL of enzyme solution [1% cellulase 'Onozuka' R10 (Yakult, Tokyo, Japan), 0.25% macerozyme 'Onozuka' R10 (Yakult), 0.4 M mannitol, 10 mM CaCl2, 20 mM KCl, 0.1% BSA and 20 mM MES, pH 5.7]. The leaves were gently shaken (40 rpm on a platform shaker) in light for 20 to 60 min until the protoplasts were released into the solution. The protoplasts were centrifuged at 100 × g for 3 min in an Eppendorff A-4-44 rotor (Hamburg, Germany), washed twice with 25 mL of pre-chilled modified W5 solution (154 mM NaCl, 125 mM CaCl2, 5 mM KCl, 5 mM glucose, and 2 mM MES, pH 5.7) and incubated on ice for 30 min. During the incubation period, protoplasts were counted using a hemocytometer under a light microscope. The protoplasts were then centrifuged and resuspended in modified MMg solution (0.4 M mannitol, 15 mM MgCl2, and 4 mM MES, pH 5.7) to a final concentration of 2 to 5 × 105 cells/mL.
Wu F.H., Shen S.C., Lee L.Y., Lee S.H., Chan M.T, & Lin C.S. (2009). Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method. Plant Methods, 5, 16.
Full text: Click here
Publication 2009
Arabidopsis Cells Cellulase Enzymes Epidermal Cells Epidermis Glucose Hyperostosis, Diffuse Idiopathic Skeletal Light Light Microscopy Magnesium Chloride Mannitol Plants Protoplasts Sodium Chloride
2
Rice Protoplast Isolation Protocol
Dehulled seeds of rice (Oryza sativa L.) cultivar Nipponbare were sterilized with 75% ethanol for 1 min. These seeds were further sterilized with 2.5% sodium hypochlorite for 20 min, washed at least five times with sterile water and then incubated on 1/2 MS medium with a photoperiod of 12 h light (about 150 μmol m-2 s-1) and 12 h dark at 26°C for 7-10 days. Green tissues from the stem and sheath of 40-60 rice seedlings were used. A bundle of rice plants (about 30 seedlings) were cut together into approximately 0.5 mm strips with propulsive force using sharp razors. The strips were immediately transferred into 0.6 M mannitol for 10 min in the dark. After discarding the mannitol, the strips were incubated in an enzyme solution (1.5% Cellulase RS, 0.75% Macerozyme R-10, 0.6 M mannitol, 10 mM MES at pH 5.7, 10 mM CaCl2 and 0.1% BSA) for 4-5 h in the dark with gentle shaking (60-80 rpm). After the enzymatic digestion, an equal volume of W5 solution (154 mM NaCl, 125 mM CaCl2, 5 mM KCl and 2 mM MES at pH 5.7) was added, followed by vigorous shaking by hand for 10 sec. Protoplasts were released by filtering through 40 μm nylon meshes into round bottom tubes with 3-5 washes of the strips using W5 solution. The pellets were collected by centrifugation at 1,500 rpm for 3 min with a swinging bucket. After washing once with W5 solution, the pellets were then resuspended in MMG solution (0.4 M mannitol, 15 mM MgCl2 and 4 mM MES at pH 5.7) at a concentration of 2 × 106 cells mL-1, determined by using a hematocytometer. The viability of protoplasts was determined by the FDA staining method as described [44 (link)]. All manipulations above were performed at room temperature.
For isolating protoplasts from etiolated rice seedlings, the sterilized seeds were germinated under light for 3 days, and then moved to the dark for another 4-7 days. The isolation procedure was the same as that for isolation of green tissue protoplasts described above.
Zhang Y., Su J., Duan S., Ao Y., Dai J., Liu J., Wang P., Li Y., Liu B., Feng D., Wang J, & Wang H. (2011). A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes. Plant Methods, 7, 30.
Full text: Click here
Publication 2011
Cells Cellulase Centrifugation Digestion Enzymes Ethanol isolation Light Magnesium Chloride Mannitol MS 1-2 Nylons Oryza sativa Pellets, Drug Plant Embryos Protoplasts Seedlings Sodium Chloride Sodium Hypochlorite Staining Stem, Plant Sterility, Reproductive Tissues
3
Rice Protoplast Isolation and Transformation
Our protoplast isolation protocol was based on the protocol for maize protoplasts provided online by J. Sheen's laboratory with several changes. Rice seeds were grown as stated above. Between 7 and 14 days post germination, plants were ~4–8 inches tall. Leaf and stem tissue was cut into 0.5 mm pieces using very sharp razors. Tissue was immediately incubated in enzyme solution (0.6 M mannitol, 10 mM MES (pH 5.7), 1.5% Cellulase RS, 0.75% Macerozyme, 0.1% BSA, 1 mM CaC12, 5 mM β-mercaptoethanol and 50 μg/ml carbenicillin) for 4 h in the dark under gentle shaking (40 rpm). After incubation, protoplasts were passed through a 35 μm nylon mesh filter. One volume of W5 solution (154 mM NaCl, 125 mM CaC12, 5 mM KC1, 2 mM MES (pH 5.7)) was added and the solution was centrifuged for 5 minutes at 1500 rpm to pellet the protoplasts. Cells were re-suspended in Mmg solution [13 (link)] (0.6 M mannitol, 15 mM MgC12, 4 mM MES (pH 5.7)) for PEG-mediated transformation at 106 cells/ml. Cells were quantified using a hemocytometer. For transformation, 40% PEG (0.6 M mannitol, 100 mM CaC12, 40% v/v PEG 3350) was added to the protoplasts for 15 minutes. Cells were washed 1× with 10 volumes of W5 and then re-suspended in incubation solution (0.6 M mannitol, 4 mM MES (pH 5.7), 4 mM KC1). Cells were incubated at 28°C in the dark overnight.
Bart R., Chern M., Park C.J., Bartley L, & Ronald P.C. (2006). A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts. Plant Methods, 2, 13.
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Publication 2006
2-Mercaptoethanol Carbenicillin Cells Cellulase Enzymes Germination isolation Maize Mannitol Nylons Oryza sativa Plant Embryos Plant Leaves Plants polyethylene glycol 3350 Precursor T-Cell Lymphoblastic Leukemia-Lymphoma Protoplasts Sodium Chloride Stem, Plant Tissues
4
Cultivation and Genetic Manipulation of Escherichia coli and Clostridium Species

Escherichia coli TOP10 (Invitrogen) and E. coli CA434 [39] (link) were cultured in Luria-Bertani (LB) medium, supplemented with chloramphenicol (25 µg/ml), where appropriate. Routine cultures of C. difficile 630 Δerm[40] (link) and C. difficile R20291 were carried out in BHIS medium (brain heart infusion medium supplemented with 5 mg/ml yeast extract and 0.1% [wt/vol] L-cysteine) [41] (link). C. difficile medium was supplemented with D-cycloserine (250 µg/ml), cefoxitin (8 µg/ml), lincomycin (20 µg/ml), and/or thiamphenicol (15 µg/ml) where appropriate. A defined minimal media [18] (link) was used as uracil-free medium when performing genetic selections. A basic nutritive mannitol broth for growth assays of C. difficile strains were prepared as follows : Proteose peptone no. 2 4% [wt/vol] (BD Diagnostics, USA), sodium phosphate dibasic 0.5%[wt/vol], potassium phosphate monobasic 0.1%[wt/vol], sodium chloride, 0.2% [wt/vol], magnesium sulfate, 0.01% [wt/vol], mannitol, 0.6% [wt/vol] with final pH at +/−7.35. For solid medium, agar was added to a final concentration of 1.0% (wt/vol). Clostridium sporogenes ATCC 15579 was cultivated in TYG media [7] (link). All Clostridium cultures were incubated in an anaerobic workstation at 37°C (Don Whitley, Yorkshire, United Kingdom). Uracil was added at 5 µg/ml, and 5-Fluoroorotic acid (5-FOA) at 2 mg/ml. All reagents, unless noted, were purchased from Sigma-Aldrich.
Ng Y.K., Ehsaan M., Philip S., Collery M.M., Janoir C., Collignon A., Cartman S.T, & Minton N.P. (2013). Expanding the Repertoire of Gene Tools for Precise Manipulation of the Clostridium difficile Genome: Allelic Exchange Using pyrE Alleles. PLoS ONE, 8(2), e56051.
Full text: Click here
Publication 2013
5-fluoroorotic acid Agar Biological Assay Brain Cefoxitin Chloramphenicol Clostridium Clostridium sporogenes Cycloserine Cysteine Diagnosis Escherichia coli Genetic Selection Heart Lincomycin Mannitol potassium phosphate proteose-peptone Sodium Chloride sodium phosphate Strains Sulfate, Magnesium Thiamphenicol Uracil Yeast, Dried
5
Efficient Protoplast Transfection Protocol
Protoplasts were transfected by a modified TEAMP method [4 (link)]. Approximately 5 × 104 protoplasts (2 × 104 to 1 × 105) in 0.2 mL of MMg solution were mixed with approximately 30 (20 to 40) μg of plasmid DNA at room temperature. An equal volume of a freshly-prepared solution of 40% (w/v) PEG (MW 4000; Fluka) with 0.1 M CaCl2 and 0.2 M mannitol was added, and the mixture was incubated at room temperature for 5 min. After incubation, 3 mL of W5 solution was added slowly, the solution was mixed, and protoplasts were pelleted by centrifugation at 100 × g for 1 min (Eppendorff A-4-44 rotor). This protoplast W5 wash step was repeated twice. The protoplasts were resuspended gently in 1 mL of W5 and were incubated in 6-well plates coated with 1% BSA at room temperature for 16 hr in light.
Wu F.H., Shen S.C., Lee L.Y., Lee S.H., Chan M.T, & Lin C.S. (2009). Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method. Plant Methods, 5, 16.
Full text: Click here
Publication 2009
Centrifugation Light Mannitol Plasmids Protoplasts

Most recents protocols related to «Mannitol»

1
Overexpression of Ceres cDNA 12723147 Confers Drought Tolerance
Not available on PMC !

Example 6

Ceres cDNA 12723147 encodes an Arabidopsis putative aldo/keto reductase. Ectopic expression of Ceres cDNA 12723147 under the control of the CaMV35S promoter induces the following phenotypes:

    • Germination on high concentrations of polyethylene glycol (PEG), mannitol and abscissic acid (ABA).
    • Continued growth on high concentration of PEG, mannitol and ABA.
      Generation and Phenotypic Evaluation of T1 Lines Containing 35S::cDNA 12723147.

Wild-type Arabidopsis Wassilewskija (WS) plants were transformed with a Ti plasmid containing cDNA 12723147 in the sense orientation relative to the CaMV35S constitutive promoter. The Ti plasmid vector used for this construct, CRS338, contains PAT and confers herbicide resistance to transformed plants. Ten independently transformed events were selected and evaluated for their qualitative phenotype in the T1 generation. No positive or negative phenotypes were observed in the T1 plants.

Screens of Superpools on High PEG, Mannitol, and ABA as Surrogate Screens for Drought Tolerance.

Seeds from 13 superpools (1,200 T2 seeds from each superpool) from the CaMV35S or 32449 over-expression lines were tested on 3 drought surrogate screens (high concentrations of PEG, mannitol, and ABA) as described above. T3 seeds were collected from the resistant plants and analyzed for resistance on all three surrogate drought screens.

Once cDNA 12723147 was identified in resistant plants from each of the three surrogate drought screens, the five individual T2 events containing this cDNA (SR01013) were screened on high PEG, mannitol, and ABA to identify events with the resistance phenotype.

Superpools (SP) are referred to as SP1, SP2 and so on. The letter following the hyphen refers to the screen (P=PEG, M=mannitol, and A=ABA) and the number following the letter refers to a number assigned to each plant obtained from that screen on that superpool. For example, SP1-M18 is the 18th plant isolated from a mannitol screen of Superpool 1.

Qualitative and Quantitative Analysis of 2 Independent Events Representing 35S::cDNA 12659859 (SR01010) on PEG, Mannitol and ABA

To identify two independent events of 35S::cDNA 12659859 showing PEG, mannitol, and ABA resistance, 36 seedlings from each of two events, SR01013-01 and -02 were screened as previously described. BastaR segregation was assessed to verify that the lines contained a single insert segregating in a 3:1 (R:S) ratio as calculated by a chi-square test (Table 6-1). Both lines (01 and 02) segregated for a single insert in the T2 generation (Table 1)

TABLE 6-1
BastaR segregation for SR01013 individual events
Probability
EventResistantSensitiveTotalof Chi-test*
SR01013-01305350.14323
SR01013-02306360.24821
SR01013-01-3341360.00248**
SR01013-02-2320320.00109**
*Chi-test to determine whether actual ratio of resistant to sensitive differs form the expected 3:1 ratio.
**Significantly different than a 3:1 (R:S) ratio

Lines SR01013-01 and -02 were chosen as the two events because they had a strong and consistent resistance to PEG, mannitol and ABA. The controls were sown the same day and in the same plate as the individual lines. The PEG (Tables 6-2 and 6-3), mannitol (Tables 6-4 and 6-5) and ABA (Tables 6-6 and 6-7) segregation ratios observed for SR01013-01 and -02 are consistent with the presence of single insert as demonstrated by chi-square, similar to what we observed for BastaR resistance (Table 6-1).

The progeny from one resistant T2 plant from each of these two events were tested in the same manner as the T2. Resistance to PEG, mannitol and ABA was also observed in the T3 generation. Taken together, the segregation of resistant seedlings containing cDNA 12723147 from two events on all three drought surrogate screens and the inheritance of this resistance in a subsequent generation, provide strong evidence that cDNA 12723147 when over-expressed can provide tolerance to drought.

TABLE 6-2
Chi-square analysis assuming a 3:1 (R:S) ratio for progeny of
SR01013-01T2 containing 35S::cDNA 12723147 on PEG.
Probability
EventObservedExpectedχ2of Chi-Test
PEG Resistant22270.9260.054
PEG Sensitive1492.778
36363.704

TABLE 6-3
Chi-square analysis assuming a 3:1 (R:S) ratio for progeny of
SR01013-02 T2 containing 35S::cDNA 12723147 on PEG.
Probability
EventObservedExpectedχ2of Chi-Test
PEG Resistant26270.037.700
PEG Sensitive109.111
3636.148

TABLE 6-4
Chi-square analysis assuming a 3:1 (R:S) ratio for progeny of
SR01013-01 T2 containing 35S::cDNA 12723147 on mannitol.
Probability
EventObservedExpectedχ2of Chi-Test
Mannitol Resistant2827.037.700
Mannitol Sensitive89.111
3636.148

TABLE 6-5
Chi-square analysis assuming a 3:1 (R:S) ratio for progeny of
SR01013-02 T2 containing 35S::cDNA 12723147 on mannitol.
Probability
EventObservedExpectedχ2of Chi-Test
Mannitol Resistant18273.0005
Mannitol Sensitive1899
363612

TABLE 6-6
Chi-square analysis assuming a 3:1 (R:S) ratio for progeny of
SR01013-02 T2 containing 35S::cDNA 12723147 on ABA.
EventObservedExpectedχ2Probability
ABA Resistant1324 5.0427.098
ABA Sensitive19 815.125
323220.167

TABLE 6-7
Chi-square analysis assuming a 3:1 (R:S) ratio for progeny of
SR01013-02 T2 containing 35S::cDNA 12723147 on ABA.
EventObservedExpectedχ2Probability
ABA Resistant1324 5.0427.098
ABA Sensitive19 815.125
323220.167
FIG. 5 provides the results of the consensus sequence (SEQ ID NOs: 178-200) analysis based on Ceres cDNA 12723147.

US11859195B2. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics (2024-01-02). CERES, INC. [US]. Inventors: Cory Christensen [US], Nestor Apuya [US], Kenneth A. Feldmann [US].
Full text: Click here
Patent 2024
14-3-3 Proteins Abscisic Acid Aldo-Keto Reductase Arabidopsis CERE Cloning Vectors Consensus Sequence DNA, Complementary Droughts Drought Tolerance Ectopic Gene Expression Germination Herbicide Resistance Mannitol Pattern, Inheritance Phenotype Plant Embryos Plants Plant Tumor-Inducing Plasmids Polyethylene Glycols Seedlings
2
Ticagrelor Oral Film Formulation
Not available on PMC !

EXAMPLE 7

IngredientsAmount
Ticagrelor (mg)70
Pectin(mg)200
Mannitol(mg)100
Carbopol(mg)300
Citric acid (mg)100
L-Lysine (mg)40
Purified water (ml)q.s. to 250 μl

Ticagrelor and pullulan were accurately weighed and dissolved in distilled water. This solution was mixed well followed by the addition of plasticizers and superdisintegrant. Then the resultant homogeneous solution was poured into a Petri dish (diameter 6 cm) and dried in an oven at 600 C for 24 h. The film was carefully removed from the Petri dish and cut into desired size (2×2 cm2).

US11878016B2. Methods and products for treating subjects with autism spectrum disorders (2024-01-23). NEURIM PHARMACEUTICALS (1991) LTD. [IL]. Inventors: Nava Zisapel [IL], Moshe Laudon [IL].
Full text: Click here
Patent 2024
Autism Spectrum Disorders Carbopol Citric Acid Hyperostosis, Diffuse Idiopathic Skeletal Lysine Mannitol Methoxypectin Plasticizers pullulan Ticagrelor
3
Compound (I) Modified Release Tablet Preparation
Not available on PMC !

Example 12

Protocol for Preparing the Compound (I) Core Tablet: The Compound (I) drug substance, Microcrystalline Cellulose PH 101, Mannitol 100SD, and Hypromellose K100 Premium LV (if required) are weighted and screened through a suitably sized sieve. The required quantity of each is transferred into a suitably sized blender and mechanically mixed.

The Crospovidone CL is weighed and screened through a suitably sized sieve. The required quantity of Crospovidone is transferred into the above-mentioned suitably sized container.

The sodium stearyl fumarate is weighed and screened through a suitably sized sieve. The required quantity is transferred into the above-mentioned suitably sized container and mechanically mixed. This provides the Compound (I) Modified Release Tablet Blend for compression.

The above-mentioned Compound (I) Modified Release Tablet Blend for compression is individually weighed and transferred into a tablet die for compression using a suitable tablet press. This provides the Compound (I) Modified Release Core Tablet.

This core tablet is placed into a container closure system.

US11872229B2. Modified release formulations of 2-[3-[4-amino-3-(2-fluoro-4-phenoxy-phenyl)pyrazolo[3,4-d]pyrimidin-1-yl]piperidine-1-carbonyl]-4-methyl-4-[4-(oxetan-3-yl)piperazin-1-yl]pent-2-enenitrile (2024-01-16). Principia Biopharma Inc. [US]. Inventors: Abu J. Ferdous [US], Mohammad R. Masjedizadeh [US], Wu Lin [GB].
Full text: Click here
Patent 2024
Crospovidone Hypromellose Mannitol microcrystalline cellulose Pharmaceutical Preparations Piperazine piperidine sodium stearyl fumarate Tablet Tetranitrate, Pentaerythritol
4
Freeze-Dried Ticagrelor Oral Dosage Formulation
Not available on PMC !

EXAMPLE 2

IngredientsAmount
Ticagrelor (mg)10
Gelatin (mg)40
Mannitol (mg)20
Methylparaben sodium (mg)10
Propylparaben sodium (mg)10
Zinc glycerate (mg)5
Aspartame (mg)2
Purified waterq.s. to 250 μl
    • 1) Dissolve Gelatin and other ingredients in purified water under stirring at 200-500 rpm.
    • 2) Make up the final volume of the solution using purified water.
    • 3) Mix the solution under stirring at 200 to 500 rpm for further 15 min.
    • 4) Dose the solution into each cavity of preformed blister sheets (preferably using dispensing pipette).
    • 5) Freeze the filled blisters at a temperature in the range of −20 to −110° C.
    • 6) Freeze dry the blisters in a lyophilizer.
    • 7) Place the blister sheet containing dried lyophilisates on the punched carrier web of the blister packaging machine to transport the blister sheets through the sealing station of the packaging machine
    • 8) Seal the blister with a lidding foil and punch into final blisters.

US11878016B2. Methods and products for treating subjects with autism spectrum disorders (2024-01-23). NEURIM PHARMACEUTICALS (1991) LTD. [IL]. Inventors: Nava Zisapel [IL], Moshe Laudon [IL].
Full text: Click here
Patent 2024
Aspartame Autism Spectrum Disorders Dental Caries Freezing Gelatins Mannitol methylparaben, sodium salt Phocidae propylparaben Sodium Ticagrelor Zinc
5
Vicagrel Tablet Formulation and Manufacture
Not available on PMC !

Example 5

Raw materialAmount mg/tablet
vicagrel5
pregaletinized starch57.5
mannitol22.5
low-substituted hydroxypropyl10
cellulose
hydroxypropyl methylcellulose4
waterq.s
sodium stearyl fumarate1
total200

The pulverized vicagrel was subjected to stirred mixing with pregelatinized starch, mannitol, low-substituted hydroxypropyl cellulose, and hydroxypropyl methyl cellulose in a high-shear granulator for 5 min, stirred at a linear speed of 4 m/s, and sheared with a shearer at 800 rpm, and granulated with added water, the particles were deagglomerated through a 10-mesh sieve, and dried in a fluidized bed while maintaining the bed temperature below 60° C. during drying. The particles were removed, and sized through a 24-mesh sieve, and sodium stearyl fumarate was added and mixed, and tableting was performed on a 10-punch rotary tablet press (ZP-10A, Sinopharm Longli), with a 8 mm shallow concave punch.

US11878078B2. Instant release pharmaceutical preparation of anticoagulant and preparation method therefor (2024-01-23). Jiangsu Vcare PharmaTech Co., Ltd. [CN]. Inventors: Yanlei Zhao [CN], Jianjun Zhang [CN], Xuefang Liu [CN], Yuan Gao [CN], Hongbin Sun [CN], Yanchun Gong [CN], Yongqiang Liu [CN].
Full text: Click here
Patent 2024
Cellulose Hypromellose low-substituted hydroxypropylcellulose Mannitol sodium stearyl fumarate Starch Tablet vicagrel

Top products related to «Mannitol»

1
Mannitol by Merck Group
Sourced in United States, Germany, Italy, United Kingdom, India, Spain, Sao Tome and Principe, Macao, China, Japan, Switzerland, Ireland, Australia, Israel
Mannitol is a type of sugar alcohol that is commonly used in the pharmaceutical industry as an excipient in various drug formulations. It is a white, crystalline powder that is odorless and has a sweet taste. Mannitol is known for its unique physical and chemical properties, which make it a valuable component in the production of various drug products.
2
D mannitol by Merck Group
Sourced in United States, United Kingdom, Germany, Canada, Spain, Italy, Japan, France
D-mannitol is a type of sugar alcohol commonly used in the production of pharmaceutical and laboratory equipment. It serves as a bulking agent, sweetener, and excipient in various formulations. D-mannitol is a white, crystalline powder with a sweet taste and is soluble in water. It is widely utilized in the pharmaceutical and biotechnology industries as a component in drug tablets, capsules, and other medicinal products.
3
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
4
Mannitol salt agar by Thermo Fisher Scientific
Sourced in United Kingdom, United States, Italy, France, Poland, Germany
Mannitol salt agar is a microbiological growth medium used for the selective isolation and identification of Staphylococcus species. It contains mannitol as the carbohydrate source and high concentrations of sodium chloride, which inhibit the growth of many non-Staphylococcus bacterial species.
5
Sucrose by Merck Group
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Sucrose is a disaccharide composed of glucose and fructose. It is commonly used as a laboratory reagent for various applications, serving as a standard reference substance and control material in analytical procedures.
6
D glucose by Merck Group
Sourced in United States, Germany, United Kingdom, China, Australia, France, Italy, Canada, Sao Tome and Principe, Japan, Macao, Israel, Switzerland, Spain, Belgium, India, Poland, Sweden, Denmark, Norway, Ireland, Mexico, New Zealand, Brazil, Singapore, Netherlands
D-glucose is a type of monosaccharide, a simple sugar that serves as the primary source of energy for many organisms. It is a colorless, crystalline solid that is soluble in water and other polar solvents. D-glucose is a naturally occurring compound and is a key component of various biological processes.
7
Bovine serum albumin (bsa) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico
Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
8
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
9
Dimethyl sulfoxide (dmso) by Merck Group
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

More about "Mannitol"

Mannitol, also known as D-mannitol, is a naturally occurring sugar alcohol found in various plants and fungi.
It has diverse applications in the pharmaceutical, food, and cosmetic industries.
Mannitol is commonly used as a diuretic, laxative, and osmotic agent in the treatment of various medical conditions, including increased intracranial pressure, glaucoma, and cerebral edema.
It also serves as a bulking agent and sweetener in the formulation of medications, food products, and personal care items.
Mannitol is known for its low caloric value and ability to maintain moisture, making it a popular choice in sugar-free and moisture-retaining formulations.
Fetal bovine serum (FBS) and bovine serum albumin (BSA) are commonly used in cell culture media, such as Dulbecco's Modified Eagle Medium (DMEM), to provide essential nutrients and growth factors for cells.
Sucrose and D-glucose are also important carbohydrate sources in cell culture and various food and pharmaceutical applications.
Research on the efficacy and reproducibility of mannitol-based products is crucial for optimizing its clinical and commercial utilization.
PubCompare.ai provides an AI-driven platform to quickly locate and compare mannitol research protocols from literature, preprints, and patents, enhancing the accuracy and reproducibility of mannitol experiments.
By utilizing this tool, researchers can improve the quality and consistency of their mannitol-related studies and formulations.