The largest database of trusted experimental protocols

Meloxicam

Meloxicam is a nonsteroidal anti-inflammatory drug (NSAID) used to treat pain and inflammation associated with osteoarthritis, rheumatoid arthritis, and other musculoskeletal conditions.
It works by reducing the production of prostaglandins, which are chemicals that contribute to inflammation and pain.
Meloxicam is typically taken orally, but it may also be administered intravenously or as a topical gel.
When used as directed, it can provide effective relief of symptoms and improved mobility for patients.
However, as with any medication, Meloxicam may have side effects, such as gastrointestinal distress, and should be used with caution, especially in elderly or high-risk patients.
Reserchers can use PubCompare.ai's AI-driven platform to effortlessly locate and compare protocols from literature, preprints, and patents, helping to identify the most accurate and reproducible methods for Meloxicam studies and enhancing their research.

Most cited protocols related to «Meloxicam»

The following mouse strains were used: GENSAT ChAT-GFP Tg(Chat-EGFP)GH293Gsat/Mmcd (#000296-UCD) and Mobp-GFP Tg(Mobp-EGFP)103Gsat/Mmcd (#030483-UCD)5 (link) (MMRRC; www.mmrrc.org); GFPM20 (link); SST-ires-Cre::Ai96 (link); and wild type C57BL/6J (Jax mice; # 000664). As anatomical tracers, we used CTB Alexa Fluor-488 (Invitrogen; #C22841; 0.5 % wt/vol in phosphate buffer) and AAV-GFP with synapsin promoter21 (link), 22 (link). AAV was produced at the Salk vector core (http://vectorcore.salk.edu) as a chimeric 1/2 serotype23 (link), purified by iodoxinal gradient and concentrated to 5.3 × 1011 genomic copy per ml. Stereotaxic injections of the tracers were done as described24 (link). Briefly, the mice were anaesthetized by 1% isoflurane inhalation. A small craniotomy (approximately 300 × 300 μm) was opened over the left primary somatosensory cortex and ~50 nl of virus or 50 nl of 0.05 % CTB Alexa FluorR 488 was injected into layer 2/3 barrel cortex at stereotaxic coordinates: caudal 1.6, lateral 3.2, ventral 0.3 mm relative to bregma. The skin incision was then closed with silk sutures, and the mice were allowed to recover with free access to food and water (meloxicam was given at 1 mg/kg, s.c. for analgesia). The brains were prepared for imaging 10–14 days later (see below).
The mouse brains were prepared for STP tomography as follows. The mice were deeply anesthetized by intraperitoneal (i.p.) injection of the mixture of ketamine (60 mg/kg) and medetomidine (0.5 mg/kg) and transcardially perfused with ~15 ml cold saline (0.9 % NaCl) followed by ~30 ml cold neutral buffered formaldehyde (NBF, 4% w/v in phosphate buffer, pH 7.4). The brains were dissected out and post-fixed in 4% NBF overnight at 4 °C. In order to decrease formaldehyde-induced autofluorescence, the brains were incubated in 0.1 M glycine (adjusted to pH 7. 4 with 1M Tris base) at 4 °C for 2–5 days. The brains were then washed in phosphate buffer (PB) and embedded in 3–5% oxidized agarose as described25 , 26 (link). Briefly, agarose (Sigma, cat.# A6013) was oxidized by stirring in 10 mM sodium periodate (NaIO4, Sigma cat.# S1878) solution for 2 hrs at RT, washed 3x and re-suspended in PB to bring the final concentration to 3–5 %. The mouse brain was pat-dried and embedded in melted oxidized agarose using a cube-shaped mold. Covalent crosslinking between brain surface and agarose was activated by equilibrating in excess of 0.5–1 % sodium borohydrate (NaBH4, Sigma cat.# 452882) in 0.05 M sodium borate buffer (pH = 9.0–9.5), gently shaking for 2–4 hrs at RT (or overnight at 4 °C) (note that after rinsing, activated agarose can be stored in PB at 4 °C for up to one week; sodium borohydrate buffer should be prepared fresh). Covalent crosslinking of the agar-brain interface is important to keep the brain firmly embedded during sectioning and to limit shadowing artifacts by insufficiently cut meninges (see Troubleshooting, below).
Publication 2011
Agar alexa fluor 488 Brain Buffers Chimera Cloning Vectors Cold Temperature Cortex, Cerebral Craniotomy Diencephalon Food Formaldehyde Fungus, Filamentous Genome Glycine Hypernatremia Inhalation Internal Ribosome Entry Sites Isoflurane Ketamine Management, Pain Medetomidine Meloxicam Meninges Mice, House mitomycin C-dextran Normal Saline Phosphates Saline Solution Sepharose Silk Skin sodium borate sodium borohydride sodium metaperiodate Somatosensory Cortex, Primary Strains Sutures Synapsins Tomography Tromethamine Virus
All animal procedures were approved by the University of British Columbia Committee on Animal Care (protocol # A11-0225) and were carried in strict accordance with the Canadian Council on Animal Care guidelines. Male C57Bl/6 mice (mean ± SD body weight 33.9 ± 4.6 g) at 4 months of age were housed with a reversed 12h light-12h dark cycle for at least 10 days before TBI. Animals were anaesthetized with isoflurane (induction: 4.5%, maintenance: 2.5-3%) in oxygen (0.9 L/min). Lubricating eye ointment was applied to prevent corneal drying. Meloxicam (1 mg/kg) and saline (1 mL/100 g body weight) were administered by subcutaneous injections for pain control and hydration, respectively. Animals were placed supine in the holding bed such that the top of the animal’s head lay flat over a hole in the head plate, aligned using crosshairs such that the piston strikes the vertex of the head covering a 5 mm area surrounding the bregma (Figure 9B and C). Impact was induced by pressing a trigger button that simultaneously fires the piston and when connected, activates a high-speed camera to record the resulting head trajectory. Isoflurane delivery was immediately stopped and the animal was continuously monitored until fully ambulatory. Total duration of isoflurane exposure was ~ 4–8 min. Twenty-four hours after the first impact, a second identical impact was delivered. Sham animals underwent all of these procedures, except for the impact. Approximately 3% of animals did not regain consciousness for > 45 min or displayed severe motor dysfunction after TBI, and were thus euthanized.
Full text: Click here
Publication 2014
Animals Body Weight Consciousness Cornea Fires Head Isoflurane Males Management, Pain Meloxicam Mice, Inbred C57BL Obstetric Delivery Ointments Oxygen Precipitating Factors Saline Solution Subcutaneous Injections

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2014
Analgesics Anesthesia Animals Cerebral Hemisphere, Right Cranium Ear Ethilon Hydrochloride, Buprenorphine Immobilization Ketamine Lactated Ringer's Solution Meloxicam Mice, House Microscopy Needles Operative Surgical Procedures Prefrontal Cortex Sutures Syringes Taxis Response Tissues Tooth Viral Genome Xylazine
Viral injections were performed using previously described procedures6 (link) at the following stereotaxic coordinates: pPVT, –1.34 mm from Bregma, 0.05 mm lateral from midline, and 3.03 mm vertical from cortical surface; CeL, −1.22 mm from Bregma, 2.9 mm lateral from midline, and 4.6 mm vertical from cortical surface; BLA, –1.80 mm from Bregma, 3.4 mm lateral from midline, and 5.4 mm vertical from cortical surface. For pPVT injections we used a 6.5° angle to avoid damage of the superior sagittal sinus. Animals were kept on a heating pad throughout the entire surgical procedures and were brought back to their home cages after 24 h post-surgery recovery and monitoring. Postoperative care included intraperitoneal injection with 0.3–0.5 ml of lactated Ringers solution and metacam (meloxicam, 1–2 mg/kg) for analgesia and anti-inflammatory purposes. All AAVs and the CAV2-Cre were injected at a total volume of approximately 1 μl (except for the monosynaptic rabies viral tracing, see below), and were allowed at least two weeks for maximal expression. For retrograde tracing of amygdala-projecting pPVT cells, CTB-555 or CTB-488 (0.1-0.3 μl, 0.5% in PBS) (Invitrogen) was injected into CeL and BLA and allowed 3-5 days for sufficient retrograde transport.
Publication 2014
Amygdaloid Body Animals Anti-Inflammatory Agents CAV2 protein, human Cells Kidney Cortex Lactated Ringer's Solution Management, Pain Meloxicam Operative Surgical Procedures Postoperative Care Rabies virus Sinus, Superior Sagittal
Mice were anaesthetised with isoflurane (3% induction, 0.5–1% maintenance), treated with buprenorphine (0.1 mg/kg) and meloxicam (5 mg/kg), and placed in a stereotactic frame. The skull was exposed and holes drilled to allow virus injection and implantation of fiber optic cannula. Mice were given additional doses of meloxicam each day for 3 days after surgery, and were monitored carefully for 7 days post-surgery.
For VTA cell body recordings (Fig. 5a,b), GCaMP and tdTomato were expressed in VTA dopamine neurons using AAV1-Syn-Flex-GCaMP6f-WPRE-SV40 (titer 6.2 × 1013) and AAV1-CAG-Flex-tdTomato-WPRE-bGH (titer 3.1 × 1013) viruses (Penn Vector Core) in male B6.SJL-Slc6a3tm1.1(cre)Bkmn/J mice. The viruses were mixed and diluted in a ratio of 20% GCaMP6f, 10% TdTomato, 70% saline. 500 nL per hemisphere of the diluted virus was injected at 1 nL/second at AP: −3.3, ML: ±0.4, DV: −4.5 mm relative to bregma. Recordings were made through a 200 um 0.53 NA fiber optic cannula implanted at AP: −3.3, ML: +0.4, DV: −4.3 mm relative to bregma.
For the NAc terminal recordings (Fig. 5c,d) GCaMP was expressed in VTA dopamine neurons using AAV1-Syn-Flex-GCaMP6f-WPRE-SV40 (titer 6.2 × 1013). The virus was diluted 1:10 with saline and 1 μL was injected per hemisphere at AP: −3.3, ML: ±0.4, DV: −4.2 mm relative to bregma. Optic fiber cannula were implanted at AP: 1.4, ML 0.8, DV: 4.1 relative to bregma.
Prior to recording, mice were put on a water restriction schedule where on training days they received 0.5–1.5 mL water from rewards received in the task and on non-training days 1 hour of unrestricted access in their home cage. Mice maintained a typical body weight of >90% pre-restriction levels. Experiments were carried out in accordance with the Oxford University animal use guidelines and performed under UK Home Office Project Licence P6F11BC25.
VTA cell body recordings were acquired using the ‘2 colour time division’ acquisition mode using 470 and 560 nm wavelength LEDs respectively for the GCaMP and tdTomato excitation light and 500–540 and 600–680 nm emission filters for the GCaMP and tdTomato signals. NAc terminal recordings were acquired using the ‘1 colour time division’ acquisition mode using 470 and 405 nm wavelength LEDs respectively for the GCaMP and isosbestic signals.
Acquired signals were bandpass filtered between 0.01 and 20 Hz using a fourth order zero phase filter. The full set of optical components used is listed Table 1 as well as in the hardware repository. The Newport photoreceivers were used in DC coupled mode for all recordings.
Full text: Click here
Publication 2019
Animals Body Weight Buprenorphine Cannula Cannulation Cell Body Cloning Vectors Cranium Dopaminergic Neurons Isoflurane Light Males Meloxicam Mus Operative Surgical Procedures Reading Frames Saline Solution Simian virus 40 tdTomato Virus

Most recents protocols related to «Meloxicam»

Example 6

A lidocaine preservative free intranasal formulation with combination of other drugs is prepared using the ingredients set forth in Table 4 for Examples 6-8.

TABLE 4
Example 6Example 7Example 8
Compositionmg/spraymg/spraymg/spray
Lidocaine101010
Epinephrine0.01
Meloxicam15
Ketamine15
Citric acid monohydrate   3.503.253.0
Purified WaterQsQsQs

The formulation is prepared as follows: Add citric acid monohydrate to purified water while stirring and mix till a clear solution is observed. Add lidocaine base or salt, combination drug and other optional excipients while stirring and mix for 30 minutes till a clear solution is formed. Filter the clear solution using sterile 0.2 micron pore size filter and fill the solution in a glass bottle aseptically and tightly crimp metered dose mechanical pump.

Full text: Click here
Patent 2024
Citric Acid Monohydrate Drug Combinations Epinephrine Excipients Ketamine Lidocaine Meloxicam Pharmaceutical Preservatives Sodium Chloride Sterility, Reproductive

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Acetaminophen Anesthesia Anesthesia, Conduction Anesthesiologist Antibiotics, Antitubercular Aprepitant Aspirin Bupivacaine Cefazolin Cephalexin Chemoprevention Chlorhexidine chlorhexidine gluconate Clindamycin Deep Vein Thrombosis Dexamethasone Ethanol Famotidine Fentanyl Gabapentin Hypersensitivity Ibuprofen Isopropyl Alcohol Management, Pain Medical Devices Meloxicam Nerve Block Ondansetron Operative Surgical Procedures Oxycodone Pain, Postoperative Patients Penicillins Percocet Postoperative Nausea Powder Ropivacaine Scopolamine Skin Surgery, Day Therapeutics Thigh Treatment Protocols Ultrasonics Vancomycin Wounds
In Yuan et al study,[29 (link)] patients received standardized general anesthesia and basic analgesic protocol. Intraoperatively, all patients received general anesthesia which was induced by sufentanil 0.5 μg/kg, midazolam 0.04 mg/kg, propofol 1 to 2 mg/kg, and Cisatracurium 2 μg/kg intravenously, followed by continuous intravenous infusion of remifentanil 0.1 to 0.3 μg/(kg·min), propofol 2 to 5 mg/(kg·hr) and inhalation of sevoflurane to maintain anesthesia. Since postoperative day 1, the protocol of oral celecoxib restarted till postoperative 3 weeks when the patients came back to the hospital for taking out the stitches. In Yadeau et al 2016 study,[6 (link)] patients received a standardized anesthetic and multimodal analgesic protocol. In Yadeau et al 2022 study,[28 (link)] patients received a standard intraoperative and postoperative multimodal anesthetic protocol: a spinal-epidural (subarachnoid mepivacaine, 45–60 mg); adductor canal block (ultrasound-guided; 15 cc bupivacaine, 0.25%, with 2 mg preservative-free dexamethasone). For postoperative pain management, patients were scheduled to receive the study medication once daily for 14 days; 4 doses of 1000 mg IV acetaminophen every 6 hours followed by 1000 mg oral acetaminophen every 8 hours; 4 doses of 15 mg IV ketorolac followed by 15 mg meloxicam every 24 hours; and 5 to 10 mg oral oxycodone was given as needed for pain. Patients could have pain medications adjusted as indicated. In Koh et al study,[12 (link)] all patients had a postoperative intravenous patient-controlled anesthesia (PCA) pump that administered 1 mL of a 100-mL mixture containing 2000 mg of fentanyl on demand. In Kim et al study,[27 ] all patients received intravenous PCA encompassing delivery of 1 mL of a 100 mL solution containing 2000 µg of fentanyl postoperatively. In Ho et al study,[26 (link)] patients were routinely offered a single shot spinal anesthesia consisting of an intrathecal dose of bupivacaine 10 to 12.5 mg with fentanyl 10 mg. After surgery, pain treatment consisted of PCA with intravenous injection of morphine. The settings were 1 mg bolus, 5 minutes lockout time, and a maximum hourly limit of 8 mg. All patients were also given acetaminophen 1 g 6 hourly.
Publication 2023
Acetaminophen Analgesics Anesthesia Anesthesia, Intravenous Anesthetics Bupivacaine Cardiac Arrest Celecoxib cisatracurium Dexamethasone Fentanyl General Anesthesia Inhalation Intravenous Infusion Ketorolac Management, Pain Meloxicam Mepivacaine Midazolam Morphine Multimodal Imaging Obstetric Delivery Operative Surgical Procedures Oxycodone Pain Pain, Postoperative Patients Pharmaceutical Preparations Pharmaceutical Preservatives Propofol Pulp Canals Remifentanil Sevoflurane Spinal Anesthesia Subarachnoid Space Sufentanil Ultrasonography
We stereotactically implanted two microdrives in each bird. The electrodes were positioned in NCL [anteroposterior (AP), +5.0; mediolateral (ML), –7.5; dorsoventral (DV), –1.5] and NIML (AP, +9.5; ML, –3.5; DV, –2.3) of the right hemisphere (Karten and Hodos, 1967 ). Coordinates for the regions were based on histologic studies on the localization of NCL (Waldmann and Güntürkün, 1993 (link); Herold et al., 2011 (link)) and NIML (Rehkämper et al., 1985 (link)). The birds were anesthetized using isoflurane and received meloxicam (2 mg/kg, i.m.) for analgesia. The skull was exposed, and small craniotomies were made over the target structures. Electrodes and microdrives were fixated with dental acrylic to small bone screws, one of which served as a ground for the recordings. After surgery, the birds received several days of recovery, with monitoring and analgesic treatment of butorphanol (1.5 ml/kg, i.m.). In one of the birds, the left hemisphere was also implanted with the same coordinates in a separate surgery.
Full text: Click here
Publication 2023
Analgesics Aves Bone Screws Butorphanol Craniotomy Cranium Dental Health Services Isoflurane Management, Pain Meloxicam Operative Surgical Procedures

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Animals Buprenorphine Cannula Catheters Cefazolin Cocaine Cranium Dental Health Services Heparin Isoflurane Jugular Vein Meloxicam Operative Surgical Procedures Polyurethanes Saline Solution Scapula Self Administration Sterility, Reproductive Ventricle, Lateral

Top products related to «Meloxicam»

Sourced in Germany, United Kingdom, Spain, United States, France, Canada, Australia, Japan, Poland, Switzerland, Italy
Metacam is a veterinary pharmaceutical product manufactured by Boehringer Ingelheim. It contains the active ingredient meloxicam, which is a nonsteroidal anti-inflammatory drug (NSAID).
Sourced in Germany, Japan, United Kingdom, Austria, United States, Canada
Meloxicam is a non-steroidal anti-inflammatory drug (NSAID) used as a laboratory tool. It acts as a selective inhibitor of the cyclooxygenase-2 (COX-2) enzyme. Meloxicam is commonly used in research settings to study inflammatory processes and related pharmacological effects.
Sourced in United States, Germany, United Kingdom
Meloxicam is a non-steroidal anti-inflammatory drug (NSAID) used as a veterinary medication. It is typically administered orally to reduce inflammation and pain in animals.
Sourced in United States, Sao Tome and Principe, Japan
Vetbond is a tissue adhesive product manufactured by 3M for use in veterinary applications. It is designed to quickly and effectively bond tissues together, facilitating wound closure and healing. The core function of Vetbond is to provide a reliable and secure means of joining various types of tissue, such as skin, muscle, and membranes, without the need for sutures or other invasive methods.
Sourced in United States, Germany, Italy
The Stereotaxic frame is a laboratory instrument used to immobilize and position the head of a subject, typically an animal, during surgical or experimental procedures. It provides a secure and reproducible method for aligning the subject's head in a three-dimensional coordinate system to enable precise targeting of specific brain regions.
Sourced in United States, United Kingdom
C&B Metabond is a dental laboratory product manufactured by Parkell. It is a dual-cure dental resin cement used for bonding indirect restorations, such as inlays, onlays, crowns, and bridges, to tooth structures.
Sourced in United States, United Kingdom, Spain, Israel, Belgium, Australia
IsoFlo is a laboratory equipment product offered by Abbott. It is designed for use in clinical and research settings. IsoFlo serves as a tool for analysis and measurement, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach.
Sourced in Germany, United States, United Kingdom, Italy, Belgium, France, Denmark, Canada, Japan, Brazil
Baytril is a veterinary antibiotic product produced by Bayer. It contains the active ingredient enrofloxacin, which is a fluoroquinolone antibiotic. Baytril is used to treat bacterial infections in animals.
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
Sourced in United States, China, Germany, Japan, Canada, United Kingdom, France, Italy, Morocco, Sweden
Male Sprague-Dawley rats are a widely used laboratory animal model. They are characterized by their large size, docile temperament, and well-established physiological and behavioral characteristics. These rats are commonly used in a variety of research applications.

More about "Meloxicam"

Meloxicam is a nonsteroidal anti-inflammatory drug (NSAID) commonly used to treat pain and inflammation associated with osteoarthritis, rheumatoid arthritis, and other musculoskeletal conditions.
It works by reducing the production of prostaglandins, which are chemicals that contribute to inflammation and discomfort.
This medication is typically taken orally, but it can also be administered intravenously or as a topical gel.
When used as directed, meloxicam can provide effective relief of symptoms and improve mobility for patients.
However, like any medication, it may have side effects such as gastrointestinal distress and should be used with caution, especially in elderly or high-risk individuals.
Researchers can utilize PubCompare.ai's AI-driven platform to effortlessly locate and compare protocols from literature, preprints, and patents.
This can help identify the most accurate and reproducible methods for meloxicam studies, enhancing the quality and reliability of the research.
The platform's powerful tools can also be used to explore related topics, such as the use of Metacam (another brand name for meloxicam) in veterinary medicine, the application of Vetbond for surgical procedures, and the incorporation of Stereotaxic frames and C&B Metabond in animal studies.
Additionally, researchers may find information on the use of anesthetics like IsoFlo and antimicrobials like Baytril, as well as the potential benefits of DMSO as a solvent for meloxicam studies.
To further optimize their research, scientists can utilize PubCompare.ai's AI-driven platform to explore the latest developments in meloxicam studies, including the use of Male Sprague-Dawley rats as common model organisms.
By leveraging the insights and comparative analysis provided by PubCompare.ai, researchers can enhance the efficiency, accuracy, and reproducibility of their meloxicam-related investigations, leading to more meaningful and impactful findings.