The mouse brains were prepared for STP tomography as follows. The mice were deeply anesthetized by intraperitoneal (i.p.) injection of the mixture of ketamine (60 mg/kg) and medetomidine (0.5 mg/kg) and transcardially perfused with ~15 ml cold saline (0.9 % NaCl) followed by ~30 ml cold neutral buffered formaldehyde (NBF, 4% w/v in phosphate buffer, pH 7.4). The brains were dissected out and post-fixed in 4% NBF overnight at 4 °C. In order to decrease formaldehyde-induced autofluorescence, the brains were incubated in 0.1 M glycine (adjusted to pH 7. 4 with 1M Tris base) at 4 °C for 2–5 days. The brains were then washed in phosphate buffer (PB) and embedded in 3–5% oxidized agarose as described25 , 26 (link). Briefly, agarose (Sigma, cat.# A6013) was oxidized by stirring in 10 mM sodium periodate (NaIO4, Sigma cat.# S1878) solution for 2 hrs at RT, washed 3x and re-suspended in PB to bring the final concentration to 3–5 %. The mouse brain was pat-dried and embedded in melted oxidized agarose using a cube-shaped mold. Covalent crosslinking between brain surface and agarose was activated by equilibrating in excess of 0.5–1 % sodium borohydrate (NaBH4, Sigma cat.# 452882) in 0.05 M sodium borate buffer (pH = 9.0–9.5), gently shaking for 2–4 hrs at RT (or overnight at 4 °C) (note that after rinsing, activated agarose can be stored in PB at 4 °C for up to one week; sodium borohydrate buffer should be prepared fresh). Covalent crosslinking of the agar-brain interface is important to keep the brain firmly embedded during sectioning and to limit shadowing artifacts by insufficiently cut meninges (see Troubleshooting, below).
Meloxicam
It works by reducing the production of prostaglandins, which are chemicals that contribute to inflammation and pain.
Meloxicam is typically taken orally, but it may also be administered intravenously or as a topical gel.
When used as directed, it can provide effective relief of symptoms and improved mobility for patients.
However, as with any medication, Meloxicam may have side effects, such as gastrointestinal distress, and should be used with caution, especially in elderly or high-risk patients.
Reserchers can use PubCompare.ai's AI-driven platform to effortlessly locate and compare protocols from literature, preprints, and patents, helping to identify the most accurate and reproducible methods for Meloxicam studies and enhancing their research.
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For VTA cell body recordings (Fig.
For the NAc terminal recordings (Fig.
Prior to recording, mice were put on a water restriction schedule where on training days they received 0.5–1.5 mL water from rewards received in the task and on non-training days 1 hour of unrestricted access in their home cage. Mice maintained a typical body weight of >90% pre-restriction levels. Experiments were carried out in accordance with the Oxford University animal use guidelines and performed under UK Home Office Project Licence P6F11BC25.
VTA cell body recordings were acquired using the ‘2 colour time division’ acquisition mode using 470 and 560 nm wavelength LEDs respectively for the GCaMP and tdTomato excitation light and 500–540 and 600–680 nm emission filters for the GCaMP and tdTomato signals. NAc terminal recordings were acquired using the ‘1 colour time division’ acquisition mode using 470 and 405 nm wavelength LEDs respectively for the GCaMP and isosbestic signals.
Acquired signals were bandpass filtered between 0.01 and 20 Hz using a fourth order zero phase filter. The full set of optical components used is listed Table
Most recents protocols related to «Meloxicam»
Example 6
A lidocaine preservative free intranasal formulation with combination of other drugs is prepared using the ingredients set forth in Table 4 for Examples 6-8.
The formulation is prepared as follows: Add citric acid monohydrate to purified water while stirring and mix till a clear solution is observed. Add lidocaine base or salt, combination drug and other optional excipients while stirring and mix for 30 minutes till a clear solution is formed. Filter the clear solution using sterile 0.2 micron pore size filter and fill the solution in a glass bottle aseptically and tightly crimp metered dose mechanical pump.
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More about "Meloxicam"
It works by reducing the production of prostaglandins, which are chemicals that contribute to inflammation and discomfort.
This medication is typically taken orally, but it can also be administered intravenously or as a topical gel.
When used as directed, meloxicam can provide effective relief of symptoms and improve mobility for patients.
However, like any medication, it may have side effects such as gastrointestinal distress and should be used with caution, especially in elderly or high-risk individuals.
Researchers can utilize PubCompare.ai's AI-driven platform to effortlessly locate and compare protocols from literature, preprints, and patents.
This can help identify the most accurate and reproducible methods for meloxicam studies, enhancing the quality and reliability of the research.
The platform's powerful tools can also be used to explore related topics, such as the use of Metacam (another brand name for meloxicam) in veterinary medicine, the application of Vetbond for surgical procedures, and the incorporation of Stereotaxic frames and C&B Metabond in animal studies.
Additionally, researchers may find information on the use of anesthetics like IsoFlo and antimicrobials like Baytril, as well as the potential benefits of DMSO as a solvent for meloxicam studies.
To further optimize their research, scientists can utilize PubCompare.ai's AI-driven platform to explore the latest developments in meloxicam studies, including the use of Male Sprague-Dawley rats as common model organisms.
By leveraging the insights and comparative analysis provided by PubCompare.ai, researchers can enhance the efficiency, accuracy, and reproducibility of their meloxicam-related investigations, leading to more meaningful and impactful findings.