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Methylene Blue

Methylene blue is a synthetic dye with a wide range of applications in biomedical research and clinical settings.
It is a heterocyclic aromatic compound that exhibits photosensitizing properties and has been studied for its potential therapeutic effects in a variety of conditions, including neurological disorders, oxidative stress, and antimicrobial applications.
Methylene blue research is an active area of investigation, with scientists exploring its mechanisms of action and optimizing protocols to enhance reproducibility and accuracy.
PubCompare.ai, an AI-driven platform, can help researchers locate the best protocols from literature, pre-prints, and patents, while providing insightful comparisons to improve research outcomes.
This tool can be a valuable resource for those interested in unlocking the power of methylene blue research.

Most cited protocols related to «Methylene Blue»

While the current standard tests of serology and conventional histology are usually adequate to reach a diagnosis of CD, there are patients whose tests are equivocal and diagnostic uncertainty remains. Several novel diagnostic approaches have been undertaken. The deposition of IgA antibodies in close proximity to TG2 in the small intestine has shown promise as a way of defining early or potential CD in patients who are seropositive but lack any of the usual histological markers for CD. Recent work from Finland on IgA-TG2 autoantibody deposition in the small intestine in such patients shows promise in delivery of an early prediction of development of CD. However, this is currently experimental and the methodology requires tissue sections frozen in liquid nitrogen.124 (link) Another diagnostic method meriting further evaluation is EmA assay in the culture medium of small intestinal biopsies.125 (link)
126 (link) Other investigators have reported their findings using new techniques associated with endoscopy to enhance the diagnosis of CD. These include confocal microscopy, high-resolution magnification endoscopy, optical band imaging127 (link) and optimal coherence tomography. These novel techniques are still limited by availability, tolerability and cost.98 (link) However, the immersion technique and dye enhancement in which the endoscopist instills water or a contrast dye (for example, indigo carmine or methylene blue) into the bowel lumen, with or without the assistance of magnification endoscopy, enhancing the visualisation of the villus128 (link) can be readily used and improves visualisation of villi, thus increasing the sensitivity for detection of villous atrophy.98 (link)
Publication 2014
Atrophy Autoantibodies Biological Assay Biopsy Culture Media Diagnosis Endoscopy Freezing Hypersensitivity Immunoglobulin A Indigo Carmine Intestines Intestines, Small Methylene Blue Microscopy, Confocal Nitrogen Obstetric Delivery Patients Submersion Tests, Serologic Tissues Tomography Vision
Mouse embryonic stem cells were cultured in M15 media (Knock-out DMEM (Gibco) and supplemented with 15% fetal bovine serum (HyClone), GPS (glutamate-penicillin-streptomycin, Gibco) and 0.1 mM β-mercaptoethanol with SNL feeder cells. 25μg BACμ DNA was electroporated into 107 Pl2F7 ES-cells (for details on generation of these cells from the AB2.2 cell line see Supplementary Figures 1–3 online) in 0.9 ml PBS at 230 V, 500 μF. Transgenic colonies were selected with G418 (Gibco) and analyzed for the presence of BRCA2 gene by Southern blot. Genomic DNA was hybridized with a 581 bp fragment corresponding to intron 1 of BRCA2 as a 5 probe (nucleotides 13,869,785–13,870,365 of NT_024524.13) recognizing a 1,835 bp EcoRI fragment and a 356 bp fragment corresponding to exon 27 of BRCA2 as a 3′ probe (nucleotides 13,953,046–13,953,401 of NT_024524.13) recognizing a 4,275 bp EcoRI fragment (Fig. 1b). Double-positive clones were further tested for expression of BRCA2 by RT-PCR and Western blot analysis. BRCA2 expressing clones were electroporated with Pgk-Cre plasmid4 (link) and 105 cells were seeded in a 100 mm dish with SNL-feeder cells. Recombinant colonies were selected in the HAT media (Gibco) for 5 days followed by 2 days in HT media (Gibco). Viable colonies were analyzed by Southern blot to confirm the loss of the conditional Brca2 allele. A 1.5 kb probe specific to Brca2 exon 11 (nucleotides 5208–6710 of NM 009765) has been used to detect a 2.2 kb EcoRV fragment corresponding to the mutant allele (ko) and a 4.8 kb fragment corresponding to the wild-type or conditional allele (cko) (Fig. 1e and Supplementary Figure 3 online). HATr colonies were also stained with methylene blue (2% methylene blue w/v in 70% ethanol for 15 minutes followed by washing with 70% ethanol) to facilitate their quantification.
Publication 2008
2-Mercaptoethanol Alleles Animals, Transgenic antibiotic G 418 Blot, Southern Cell Lines Cells Clone Cells Culture Media Deoxyribonuclease EcoRI Embryonic Stem Cells Ethanol Exons Feeder Cells Fetal Bovine Serum Gene, BRCA2 Genome Glutamate Hyperostosis, Diffuse Idiopathic Skeletal Introns Loss of Heterozygosity Methylene Blue Mouse Embryonic Stem Cells Nucleotides Penicillins Reverse Transcriptase Polymerase Chain Reaction Streptomycin Western Blot
Isolation of elaA promoter (1.9 kb) and construction of the chimeric plasmid pElaA-EGFP have been described previously [17 (link)] The liver-specific promoter (2.8 kb) derived from the zebrafish liver fatty acid binding protein gene was provided by Dr. G.-M. Her and was inserted into pDsRed-Express-1 (Clontech, USA) to make the chimeric plasmid pLFABP-RFP. Both plasmids were linearized, mixed with 0.25% phenol red solution (1:1:1) at a final concentration of 100 ng/μl of each plasmid. Microinjection was carried out at the 1–2 cell stage. The DNA solution was injected into the boundary between the yolk and blastodisc. After microinjection, the embryos were maintained in egg water [29 ] with ~0.0005% methylene blue in a 28.5°C incubator. Transgenic founders were screened by observation of F1 embryos for RFP and GFP expression. 444 injected embryos were raised to adult and 66 of them were screened for transgenics. Two of them were found to produce F1 embryos with strong liver-specific RFP expression and exocrine pancreas-specific GFP expression. Thus, two stable transgenic lines were established and both showed standard Mendelian inheritance from F2 generation onwards. Since identical reporter gene expression patterns were observed in the two lines, only one line, named LiPan, was used for further characterization. Homozygous LiPan zebrafish were viable and had no visible phenotype. The LiPan line has been maintained in our laboratories for over eight generations and co-expression of RFP in the liver and GFP in the exocrine pancreas is always observed. Thus the two injected DNA constructs are likely co-integrated into the same chromosomal locus.
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Publication 2008
Adult Animals, Transgenic Blastodisc Cells Chimera Chromosomes Embryo Fatty Acid-Binding Proteins, Liver-Specific Gene Expression Genes Genes, Reporter Homozygote isolation Liver Methylene Blue Microinjections Pancreas, Exocrine Pattern, Inheritance Phenotype Plasmids Recombinant DNA Zebrafish
Embryos from wildtype (TL, AB/TU, and WIK), transgenic (Tg(lyz:EGFP)[28 (link)], Tg(mpeg1:EGFP)[29 (link)], and Tg(pu.1:Gal4-UAS-EGFP)[22 (link)]), and irf8st95 and irf8st96 heterozygous intercross backgrounds were raised at 28.5°C, and staged by established standards [40 (link)]. Embryos were treated with 0.003% 1-phenyl-2-thiourea (PTU) in methylene blue embryo water to inhibit pigmentation. For in situ hybridization and TUNEL staining, zebrafish embryos and larvae were fixed immediately at the indicated time points of analysis using 4% paraformaldehyde/PBS for overnight fixation at 4°C. For fluorescent imaging, zebrafish embryos and larvae up to 7 dpf were imaged in the living animals at the time of analysis, and older larvae up to 31 dpf were imaged after fixation; they were briefly anesthetized using 0.02% MS-222 (tricaine) prior to overnight fixation in 4% paraformaldehyde/PBS. All euthanasia and procedures followed the protocols approved by the Stanford Institutional Animal Care and Use Committee.
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Publication 2015
Animals Animals, Transgenic Cardiac Arrest Embryo Euthanasia Heterozygote In Situ Hybridization In Situ Nick-End Labeling Institutional Animal Care and Use Committees Larva Methylene Blue MPEG1 protein, human MS-222 paraform Phenylthiourea Pigmentation tricaine Zebrafish
AB2.2 ES cells were cultured on a layer of mitotically inactive SNL76/7 feeder cells and transfected by electroporation as described previously (23 (link)). Puromycin selection was conducted on SNLP 76/7-4 feeders (a puromycin resistant derivative of SNL76/7) with 3 μg/ml of puromycin. Puromycin resistant ES cell colonies were stained for 15 min with 1% methylene blue in 70% EtOH, washed in distilled water over night and air-dried. This protocol produced a very low background which allowed counting colonies with diameters ⩾0.3 mm. COS-7 cells and HeLaS3 cells were grown in 6-well plates and with DMEM supplemented with 10% foetal bovine serum. Plasmids were transfected with Lipofectamine 2000 (Invitrogen), following manufacturer's instructions.
Publication 2007
Cells COS-7 Cells Electroporation Embryonic Stem Cells Ethanol Feeder Layer Cells Fetal Bovine Serum lipofectamine 2000 Methylene Blue Plasmids Puromycin

Most recents protocols related to «Methylene Blue»

The diagnosis of anal fistula is based on the German S3 guidelines: anal abscess and fistula (23 (link)). All patients were diagnosed with anal fistula by anal finger examination, anoscope examination, radiographic examination (including rectal endoluminal ultrasound, pelvic CT, or MRI), or intraoperative probe/methylene blue staining, and the number of internal orifices was counted by these techniques. The diagnostic criteria for T2DM were based on the latest Chinese guidelines for the prevention and treatment of T2DM set by the Chinese Diabetes Society (24 (link), 25 (link)). And the diagnosis was assigned by an endocrinologist. Relevant data were collected on the cases, including demographic characteristics, clinical features, laboratory and ancillary tests at admission, anal fistula-related information (e.g., previous surgical history, anal fistula types, number of internal orifices, etc.), pre- and post-surgical treatments, and surgical modalities. Non-healing (refractory) group refers to trauma that cannot be repaired in time with conventional therapy or wounds that can not achieve functional recovery and anatomical integrity (26 (link)). The last routine dressing change time in the outpatient clinic was collected as the outcome indicator. Judged by the specialist anorectologist and the definition of the relevant literature, patients were divided into the non-healing (refractory) group or healing group according to whether its recovery period is longer than 35 days (27 (link)–29 (link)).
Among the underlying diseases, hypertensive disease and non-alcoholic fatty liver diseases are listed independently. Chronic cardiovascular diseases included coronary atherosclerotic heart disease and lacunar cerebral infarction. Chronic lung diseases included tuberculosis, chronic obstructive pulmonary disease, and chronic pulmonary heart disease. Chronic liver diseases included chronic viral hepatitis B, cirrhosis of the liver, hepatic hemangioma, etc.
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Publication 2023
Abscess Anal Fistula Anus Cardiovascular Diseases Cardiovascular System Chinese Chronic Obstructive Airway Disease Coronary Arteriosclerosis Cor Pulmonale Diabetes Mellitus Diagnosis Disease, Chronic Endocrinologists Fingers Fistula Heart Hemangioma Hepatitis B, Chronic High Blood Pressures Hospital Admission Tests Liver Liver Cirrhosis Liver Diseases Lung Lung Diseases Methylene Blue Non-alcoholic Fatty Liver Disease Operative Surgical Procedures Patients Pelvis Recovery of Function Rectum Stroke, Lacunar Therapeutics Tuberculosis Ultrasonics Wounds Wounds and Injuries X-Rays, Diagnostic
The photocatalytic behaviour was investigated by the oxidation of methylene blue and reduction of Cr(VI). The photocatalytic performance was evaluated under simulated solar light using a 300 W Xe lamp (a high-pressure 150 W xenon lamp, LOT – QuantumDesign GmbH equipped with the AM1.5G filter). The intensity of the incident light that reaches the surface of the investigated solution was equal to 100 mWcm−2 (measured using a Coherentâ FieldMate Laser Power Meter). In a typical test, 20 mg of catalyst was placed in a 50 mL aqueous pollutant solution. The concentration of MB and Cr(VI) was 1·10–5 M. Before irradiation, the suspension was vigorously stirred in the dark for 30 min to reach desorption-adsorption equilibrium. The change in MB and Cr(VI) concentration was monitored by its absorption at 665 nm and 351 nm, respectively, from the UV–Vis (Spektrofotometr UV5100) spectra of the solution, using distilled water as a reference. A total of 0.75 ml of suspension was collected and centrifuged before UV‒Vis measurement. In the case of Cr(VI) photoreduction, the process was conducted in acidified (pH = 3) solutions.
To study the reusability of the prepared photocatalysts, the cycle experiment was repeated 4 times for the photodegradation of methylene blue. After each photodegradation test, the catalyst was collected by centrifugation, dried under natural conditions and used for the next degradation experiment. Moreover, to indicate the role of hydroxyl radicals (·OH), (h +) holes and superoxide radicals (·O2-) in the process of MB degradation, experiments were performed in the presence of appropriate scavengers: t-butanol (TBA), ammonium oxalate (AO) and benzoquinone (BQ). The concentration of each scavenger was equal to 1 mM.
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Publication 2023
1,4-benzoquinone Adsorption Ammonium Oxalate blue 4 carbene Centrifugation Environmental Pollutants Gas Scavengers Hydroxyl Radical Light Methylene Blue Photodegradation Pressure Radiotherapy Superoxides tert-Butyl Alcohol Xenon
Ammonium metavanadate (NH4VO3, 99.0%), oxalic acid dihydrate (C2H2O4 × 2H2O, 97.0%), and methylene blue (MB > 98%) were obtained from Sigma‒Aldrich and used without further purification. Deionized water was used in all experiments (conductivity < 0,06 μS/cm). Graphene oxide (GO) employed in the composite synthesis was prepared using the modified Hummers method 69 (link). Potassium dichromate (K2Cr2O7, ≥ 99.0%) and ammonium oxalate (AO, ≥ 99%) were purchased from Merck. Benzoquinone (BQ, > 98%) and tert-butyl alcohol (TBA, > 99.5%) were received from CheMondis.
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Publication 2023
1,4-benzoquinone ammonium metavanadate Ammonium Oxalate Anabolism Electric Conductivity graphene oxide Methylene Blue Oxalic Acid Potassium Dichromate tert-Butyl Alcohol
gDNA samples were boiled at 95 °C for 10 min for denaturation. Samples were immediately placed on ice for 5 min, and 250–500 ng were loaded on regular nitrocellulose membranes. DNA was then autocrosslinked in a UVC 500 crosslinker – 2 times at 1200uj (*100). The membranes were blocked for 1 h in 5% PBST-BSA. Membranes were probed for 1 h at room temperature or overnight at 4 °C with 1:1000 dilution of 5mC antibody (OptimAbtm Anti-5-Methylcytosine – BY-MECY 100). 5mC signals were revealed using 1:5000 dilution of HRP conjugated antirabbit IgG secondary antibody for 1 h at room temperature followed by chemo luminescence. Loading was measured using methylene blue staining.
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Publication 2023
Immunoglobulin G Immunoglobulins Luminescence Methylene Blue Nitrocellulose Technique, Dilution Tissue, Membrane

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Publication 2023
basic fuchsin Biopharmaceuticals Ethanol Femur fluorexon Light Microscopy Methylene Blue Microscopy Microscopy, Fluorescence Paraffin paraform Polymethyl Methacrylate Rattus Tissues Vascular Endothelial Growth Factor Receptor-2 Xylene

Top products related to «Methylene Blue»

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Methylene blue is a chemical compound used as a laboratory reagent. It is a blue crystalline solid that is soluble in water and other polar solvents. Methylene blue is commonly used as an indicator in various chemical and biological assays, as well as a staining agent in microscopy.
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
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Methylene blue is a synthetic dye commonly used in various laboratory and industrial applications. It serves as a versatile reagent for a range of analytical and diagnostic procedures. Methylene blue exhibits a characteristic blue color and is known for its oxidation-reduction properties, making it a valuable tool in various laboratory settings.
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.
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Methylene blue is a synthetic dye compound commonly used in various laboratory applications. It is a dark blue or purple crystalline solid that is soluble in water and alcohol. Methylene blue's core function is as a redox indicator, exhibiting different colors based on its oxidation state.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Methyl orange is a laboratory chemical used as an acid-base indicator. It changes color from red (acidic) to yellow (basic) around pH 3.1 to 4.4, indicating the pH of a solution.
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Eosin methylene blue agar is a selective and differential culture medium used for the isolation and identification of Gram-negative enteric bacteria, particularly Escherichia coli and other coliforms. It contains eosin Y and methylene blue as indicator dyes, which differentiate between lactose-fermenting and non-lactose-fermenting bacteria based on colony color.

More about "Methylene Blue"

Methylene blue is a versatile compound with a wide range of applications in the biomedical field.
It is a synthetic dye with photosensitizing properties, making it a subject of active research for its potential therapeutic effects in various conditions.
One of the key areas of methylene blue research is its use in neurological disorders.
Researchers have been exploring the compound's mechanisms of action and its ability to alleviate oxidative stress, which is a common factor in many neurological conditions.
Studies have also investigated methylene blue's potential as an antimicrobial agent, with promising results in combating bacterial and fungal infections.
To optimize the research process and enhance reproducibility, scientists are utilizing AI-driven platforms like PubCompare.ai.
This tool helps researchers locate the best protocols from literature, preprints, and patents, while providing insightful comparisons to improve research outcomes.
By leveraging the power of AI, researchers can unlock new insights and accelerate the development of innovative methylene blue-based therapies.
Beyond its biomedical applications, methylene blue has also found use in various other fields, such as dye manufacturing, water treatment, and even as a food coloring agent.
Its versatility is further demonstrated by its structural similarities to other compounds like sodium hydroxide, hydrochloric acid, ethanol, DMSO, methanol, methyl orange, and eosin methylene blue agar, all of which have their own unique applications and properties.
By delving deeper into the research and applications of methylene blue, scientists can uncover new possibilities and advance our understanding of this remarkable compound.
With the help of tools like PubCompare.ai, the process of discovering and optimizing methylene blue-based solutions becomes more efficient and effective, ultimately leading to better research outcomes and potential breakthroughs in the field.