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Monodansylcadaverine
Monodansylcadaverine
Monodansylcadaverine is a fluorescent compound used to study autophagy, a cellular process involving the degradation and recycling of damaged or unwanted components.
It selectively labels autophagic vacuoles, allowing researchers to visualize and quantify autophagic activity.
PubCompare.ai enhances the research process for Monodansylcadaverine by providing AI-driven comparisons to help locate the most accurate and reprodcible protocols from literature, preprints, and patents.
This powerful tool can improve the accuracy and effeciency of your Monodansylcadaverine research.
It selectively labels autophagic vacuoles, allowing researchers to visualize and quantify autophagic activity.
PubCompare.ai enhances the research process for Monodansylcadaverine by providing AI-driven comparisons to help locate the most accurate and reprodcible protocols from literature, preprints, and patents.
This powerful tool can improve the accuracy and effeciency of your Monodansylcadaverine research.
Most cited protocols related to «Monodansylcadaverine»
Amiloride
anti-IgG
Cell Nucleus
Cells
Clathrin
DAPI
Endocytosis
Fabry Disease
Fluorescein-5-isothiocyanate
Goat
Intercellular Adhesion Molecules
Microscopy, Fluorescence
Microscopy, Phase-Contrast
monodansylcadaverine
Mus
Tumor Necrosis Factor-alpha
Different endocytotic inhibitors were tested for their optimal concentration, exposure time and cell impairment (Table 1 , Figure 3 ). Inhibition of clathrin-mediated endocytosis in both cell types was tested with chlorpromazine hydrochloride (C8138, Sigma-Aldrich, Switzerland) and monoDansylcadaverine (Dansylcadaverine, D4008, Sigma-Aldrich). Inhibition of caveolin-mediated endocytosis was performed in both cell lines using 10 mM methyl-β-cyclodextrin (mβcd) (C4555, Sigma-Aldrich). A 4 µM cytochalasin D (C8273, Sigma-Aldrich) solution was used to inhibit phagocytosis and macropinocytosis in both cell lines.
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Cardiac Arrest
Caveolin 1
Cell Lines
Cells
Clathrin
Cyclodextrins
Cytochalasin D
Endocytosis
Hydrochloride, Chlorpromazine
inhibitors
monodansylcadaverine
Phagocytosis
Psychological Inhibition
Wild-type
and NPD fibroblasts were pretreated for 30 min at 37 °C with
control media or media supplemented with one or a combination of the
following pharmacological inhibitors: 50 μM monodansylcadaverine
(MDC; inhibits clathrin endocytosis), 1 μg/mL filipin (inhibits
caveolar endocytosis), and 3 mM amiloride (inhibits CAM endocytosis).23 (link) Cells were then incubated for 1 h with inhibitor-supplemented
media containing 1 mg/mL Texas Red dextran (a nondegradable fluid
phase marker for endocytosis). Cells were fixed and the number of
fluorescent dextran-filled compartments were quantified by fluorescence
microscopy using an algorithm that quantifies fluorescent objects
whose intensity is above a threshold background level.21 (link)
and NPD fibroblasts were pretreated for 30 min at 37 °C with
control media or media supplemented with one or a combination of the
following pharmacological inhibitors: 50 μM monodansylcadaverine
(MDC; inhibits clathrin endocytosis), 1 μg/mL filipin (inhibits
caveolar endocytosis), and 3 mM amiloride (inhibits CAM endocytosis).23 (link) Cells were then incubated for 1 h with inhibitor-supplemented
media containing 1 mg/mL Texas Red dextran (a nondegradable fluid
phase marker for endocytosis). Cells were fixed and the number of
fluorescent dextran-filled compartments were quantified by fluorescence
microscopy using an algorithm that quantifies fluorescent objects
whose intensity is above a threshold background level.21 (link)
Amiloride
Cardiac Arrest
Cells
Clathrin
Dextran
Endocytosis
Fibroblasts
Filipin
inhibitors
monodansylcadaverine
Adenocarcinoma
Autophagosome
Autophagy
bafilomycin A1
Breast
Cells
Cytosol
Dyes
epigallocatechin gallate
Homo sapiens
Macrophage
Malignant Neoplasms
MCF-7 Cells
Microscopy
Microscopy, Fluorescence
monodansylcadaverine
Mus
paraform
RAW 264.7 Cells
Western Blot
The formation of autophagic vesicles is a marker of cell autophagy, which is labeled by monodansylcadaverine (MDC) and appeared as bright green punctate under fluorescence microscopy, and 4’,6-diamidino-2-phenylindole (DAPI) was used to label DNA in the nucleus, which appeared as a blue fluorescence. Briefly, after 2 days of culture, the cells were incubated with 0.02 mM MDC and/or DAPI (1 μg/mL) in 24-well plates at 37 °C for 20 min, immediately followed by fixation in 4% paraformaldehyde for 30 min at 4 °C. A fluorescence microscope (excitation: 390 nm, emission: 460 nm) was used to analyze the cells with bright punctate. Two hundred cells per sample were analyzed and the percentage of autophagic cells was calculated.
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Autophagy
Cell Nucleus
Fluorescence
Microscopy, Fluorescence
monodansylcadaverine
paraform
Most recents protocols related to «Monodansylcadaverine»
Monodansylcadaverine (MDC) (Sigma-Aldrich Canada, Mississauga, ON, Canada) was utilized for visualizing autophagic vacuoles. K562 and KG1α cells were seeded in six-well plates and treated with Rh2 (60 µM) for 48 hours. Subsequently, the cells were incubated with 0.1 mM MDC for 30 minutes. The samples were mounted in 50% glycerol, and uorescent images were captured at 400x magni cation using a uorescent microscope (Olympus, Tokyo, Japan).
Cell autophagy was detected by MDC staining. After washing the cells from different treatment groups once with 1xWash buffer, the cells were incubated with MDC staining reagent (Solarbio) in the dark for 30 minutes, followed by three washes with 1xWash buffer, and nally, the cells were imaged with a uorescence microscope.
HaCaT cells were exposed to UVB (100 mJ/cm2) radiation, then treated with CHS for 24 h. For SA-β-Gal (senescence-associated β-galactosidase) staining, the cells were fixed and incubated with a staining solution containing X-gal at 37 °C for two days. For the AO/EB (acridine orange/ethidium bromide) double-staining assay, the cells were stained with a mixture of AO and EB (5 μg/mL) for 10 min in PBS buffer. The MDC (monodansylcadaverine) staining was measured at an excitation wavelength of 335 nm and an emission wavelength of 512 nm. And the fluorescence images were captured using a fluorescence microscope (Zeiss, Oberkochen, Germany).
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MDC, a fluorescent compound, was used as a tracer of autophagic vacuoles. After treatment, H9C2 cells were washed twice with washing buffer and incubated with 300 μL MDC solution (50 μM) for 8–10 min. Photographs were captured with a confocal microscope (UltraVIEW® Vox, PerkinElmer, Santa Clara, CA, USA). Images were taken at 200× magnification. Data analysis was performed using Image J software.
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The autophagosomes were detected using MDC fluorescent staining kit (C3018S, Beyotime, Shanghai, China), following the manufacturer’s instructions. Cells were cultured in 24-well plates and treated with a 1:1000 dilution of MDC for 30 min at 37 °C in the absence of light. Then, the cells underwent three consecutive washes with assay buffer. The degree of autophagy was assessed utilizing a fluorescence microscope (Leica, MHG, Germany).
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Top products related to «Monodansylcadaverine»
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Monodansylcadaverine (MDC) is a fluorescent compound used in various laboratory applications. It functions as a selective stain for autophagic vacuoles and can be used to monitor autophagy in cells.
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Monodansylcadaverine is a fluorescent dye used for the detection and quantification of autophagic vacuoles in cells. It is a selective marker for autophagic vacuoles and can be used in various cell types and applications.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Acridine orange is a fluorescent dye used in various laboratory applications. It is a metachromatic dye that can detect and differentiate between DNA and RNA within cells. Acridine orange is commonly used in microscopy techniques, cell biology studies, and nucleic acid staining.
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The Olympus Fluorescence Microscope is an optical microscope that uses fluorescence to visualize and analyze samples. It illuminates the specimen with light of a specific wavelength, causing fluorescent molecules within the sample to emit light at a different wavelength, which is then detected and displayed.
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Propidium iodide is a fluorescent dye commonly used in molecular biology and flow cytometry applications. It binds to DNA and is used to stain cell nuclei, allowing for the identification and quantification of cells in various stages of the cell cycle.
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3-methyladenine (3-MA) is a chemical compound used in laboratory research. It functions as an inhibitor of autophagy, a cellular process involved in the degradation and recycling of cellular components. 3-MA is commonly used as a tool to study the role of autophagy in various biological processes and disease models.
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The Autophagy/Cytotoxicity Dual Staining Kit is a laboratory tool designed to simultaneously detect and measure the levels of autophagy and cytotoxicity within cell samples. It provides a quantitative analysis of these two cellular processes.
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β-actin is a cytoskeletal protein that is ubiquitously expressed in eukaryotic cells. It is an important component of the microfilament system and is involved in various cellular processes such as cell motility, structure, and integrity.
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Chloroquine is a laboratory chemical primarily used as a research tool in biochemical and cell biology applications. It is a white, crystalline solid that is soluble in water. Chloroquine is commonly used in experiments to study cellular processes, such as autophagy and endocytosis, by inhibiting the function of lysosomes. Its core function is to serve as a research reagent for scientific investigations, without making any claims about its intended use.
More about "Monodansylcadaverine"
Monodansylcadaverine (MDC) is a widely used fluorescent compound that helps researchers study the cellular process of autophagy.
Autophagy is the degradation and recycling of damaged or unwanted components within a cell.
MDC selectively labels autophagic vacuoles, allowing scientists to visualize and quantify autophagic activity using techniques like fluorescence microscopy.
MDC is often used in combination with other dyes and stains, such as Acridine orange, Propidium iodide, and the Autophagy/Cytotoxicity Dual Staining Kit, to provide a more comprehensive understanding of cellular processes.
Researchers may also use inhibitors like 3-methyladenine (3-MA) or Chloroquine to modulate autophagy and study its effects.
Accurate and reproducible protocols are crucial for effective MDC research.
PubCompare.ai is a powerful tool that enhances the research process by providing AI-driven comparisons of protocols from literature, preprints, and patents.
This helps researchers locate the most reliable and validated methods, improving the accuracy and efficiency of their MDC studies.
In addition to its use in autophagy research, MDC has been employed to investigate other cellular processes, such as apoptosis and cytotoxicity.
It can be a valuable tool for a wide range of biological and biomedical applications, including the study of diseases, drug development, and cellular mechanisms.
Whether you're a seasoned researcher or new to the field, understanding the versatility and applications of Monodansylcadaverine (MDC) can help advance your scientific discoveries.
Leverage the power of PubCompare.ai to optimize your MDC research and unlock new insights into cellular function and disease pathways.
Autophagy is the degradation and recycling of damaged or unwanted components within a cell.
MDC selectively labels autophagic vacuoles, allowing scientists to visualize and quantify autophagic activity using techniques like fluorescence microscopy.
MDC is often used in combination with other dyes and stains, such as Acridine orange, Propidium iodide, and the Autophagy/Cytotoxicity Dual Staining Kit, to provide a more comprehensive understanding of cellular processes.
Researchers may also use inhibitors like 3-methyladenine (3-MA) or Chloroquine to modulate autophagy and study its effects.
Accurate and reproducible protocols are crucial for effective MDC research.
PubCompare.ai is a powerful tool that enhances the research process by providing AI-driven comparisons of protocols from literature, preprints, and patents.
This helps researchers locate the most reliable and validated methods, improving the accuracy and efficiency of their MDC studies.
In addition to its use in autophagy research, MDC has been employed to investigate other cellular processes, such as apoptosis and cytotoxicity.
It can be a valuable tool for a wide range of biological and biomedical applications, including the study of diseases, drug development, and cellular mechanisms.
Whether you're a seasoned researcher or new to the field, understanding the versatility and applications of Monodansylcadaverine (MDC) can help advance your scientific discoveries.
Leverage the power of PubCompare.ai to optimize your MDC research and unlock new insights into cellular function and disease pathways.