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Morin

Morin is a flavonoid compound found in various plants, including mulberry, onions, and guava.
It has been studied for its potential antioxidant, anti-inflammatory, and neuroprotective properties.
Morin may offer therapeutic benefits in conditions such as neurodegenerative diseases, cancer, and metabolic disorders, though more research is needed to fully understand its mechanisms of action and clinical applications.
Researchers can leverage AI-powered tools like PubCompare.ai to streamline the identification and comparison of Morin-related protocols from the literature, preprints, and patents, enhancing the efficiency and reproducibility of their research in this area.

Most cited protocols related to «Morin»

1
Insomnia Severity Index: Korean Validation
Cited 92 times
The ISI comprises seven items that evaluate difficulty falling asleep and staying asleep, problems waking up too early, satisfaction with current sleep patterns, interference with daily functions, noticeability of impairment attributed to sleep problems, and distress caused by the sleep problem. Each of the ISI items is rated on a scale of 0-4; the total score ranges from 0 to 28, with a higher score indicating greater insomnia severity. The total ISI scores are divided into four subcategories: 0-7, no clinically significant insomnia; 8-14, subthreshold insomnia; 15-21, moderate insomnia; and 22-28, severe insomnia. A cutoff score of 15 has been used as the threshold for clinically significant insomnia, and a score below 8 has been used to define remission after treatment (i.e., no longer meets the criteria for insomnia).28 (link)
Linguistic validation was achieved by having two sleep specialists translate the original ISI questionnaire into Korean; the Korean version was then translated back into English by one sleep specialist and one linguist, both of whom were fluent in Korean and English. Comparison of the original ISI with the final back-translated version was performed by individuals who were fluent in both languages and who were not involved in the research study. The final ISI-K was obtained after completion of these standard procedures.
Cho Y.W., Song M.L, & Morin C.M. (2014). Validation of a Korean Version of the Insomnia Severity Index. Journal of Clinical Neurology (Seoul, Korea), 10(3), 210-215.
Publication 2014
Aftercare Dyssomnias Koreans Satisfaction Sleep Sleeplessness
2
High-Resolution Cryo-EM Imaging Protocols
Cited 87 times
For EMPIAR-10025 and EMPIAR-10096, the aligned and summed micrographs along with CTF estimates were taken directly from the public data release on EMPIAR. For EMPIAR-10028 and EMPIAR-10261, frames were aligned and summed without dose compensation using MotionCor238 . Whole micrograph CTF estimates provided with the public release were used for this dataset.
For the clustered protocadherin dataset (EMPIAR-10234), single particle micrographs were collected on a Titan Krios (Thermo Fisher Scientific) equipped with a K2 counting camera (Gatan, Inc.); the microscope was operated at 300 kV with a calibrated pixel size of 1.061 Å. 10 secs exposures were collected (40 frames/micrograph), for a total dose of 68 e–/Å2 with a defocus range of 1 to 4 μm. A total of 896 micrographs were collected using Leginon39 . Frames were aligned using MotionCor238 . 1,540 particles were picked manually using Appion Manual Picker23 (link) from 87 micrographs and used as a training dataset for Topaz.
The rabbit muscle aldolase dataset (EMPIAR-10215) was collected on a Titan Krios (Thermo Fisher Scientific) equipped with a K2 counting camera (Gatan, Inc.) in super-resolution mode; the microscope was operated at 300 kV with a calibrated super-resolution pixel size of 0.416 Å. 6 secs exposures were collected (30 frames/micrograph), for a total dose of 70.32 e–/Å2 with a defocus range of 1 to 2 μm. A total of 1,052 micrographs were collected using Leginon39 . Frames were aligned, Fourier binned by a factor of 2, and dose compensated using MotionCor238 . Whole-image CTF estimation was performed using CTFFIND440 .
The Toll receptor dataset was collected on a Titan Krios (Thermo Fisher Scientific) equipped with a K2 counting camera (Gatan, Inc.); the microscope was operated at 300 kV with a calibrated pixel size of 0.832 Å. 6 secs exposures were collected (40 frames/micrograph), for a total dose of 73.48 e-/Å2 with a defocus range of 1.5 to 2.0 μm. A total of 9,323 micrographs were collected using Leginon. Frames were aligned using MotionCor238 . Whole-image CTF estimation was performed using CTFFIND440 .
Bepler T., Morin A., Rapp M., Brasch J., Shapiro L., Noble A.J, & Berger B. (2019). Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs. Nature methods, 16(11), 1153-1160.
Publication 2019
factor A Fructosediphosphate Aldolase Microscopy Muscle Tissue Rabbits Reading Frames TLR4 protein, human Topaz resin
3
Cryo-EM Structure Determination Workflow
Cited 72 times
Reconstruction was performed using cryoSPARC25 (link). For each particle set, we first generated an ab initio structure with a single class. These structures were then refined using cryoSPARC’s “homogenous refinement” option with symmetry specified depending on the dataset (T20S proteasome: D7, 80S ribosome: C1, aldolase: D2). For the aldolase dataset, we used C2 symmetry for ab initio structure determination. Otherwise, all other parameters were left in the default setting. When evaluating the quality of Topaz particle sets for decreasing score thresholds, each particle set was selected by taking all particles predicted by the Topaz model with scores greater than or equal to the given threshold. Reconstructions were calculated for each of these sets independently as described above.
Bepler T., Morin A., Rapp M., Brasch J., Shapiro L., Noble A.J, & Berger B. (2019). Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs. Nature methods, 16(11), 1153-1160.
Publication 2019
Aldehyde-Lyases Homozygote Multicatalytic Endopeptidase Complex Reconstructive Surgical Procedures Ribosomes Topaz resin
4
Classifying miRNA Families by Conservation
Cited 62 times
When partitioning miRNA families according to their conservation level, we began with a high-confidence set of human miRNAs supported by small-RNA sequencing (T Tuschl, personal communication) that shared nucleotides 2–8 with a mouse miRNA supported by small-RNA sequencing (Chiang et al., 2010 (link)). We then extracted 100-way multiz alignments of each mature miRNA from the UCSC Genome Browser and counted the number of species for which nucleotides 2–8 of the miRNA did not change. As an initial pass, those conserved among ≥40 species were classified as mammalian conserved, and those conserved among >60 species were classified as more broadly conserved among vertebrate species. Due to poorer quality alignments for more distantly related species, this procedure misclassified several more broadly conserved miRNAs as mammalian conserved. Therefore, mammalian conserved miRNAs that aligned with >90% homology to a mature miRNA from chicken, frog, or zebrafish, as annotated in miRBase release 21 (Kozomara and Griffiths-Jones, 2014 (link)), were re-classified as more broadly conserved. In addition, miR-489 was included in the broadly conserved set of TargetScanHuman (but not TargetScanMouse) despite having a seed substitution in mouse.
Some mammalian pri-miRNAs give rise to two or three abundant miRNA isoforms that have different seeds, either because both strands of the miRNA duplex load into Argonaute with near-equal efficiencies or because processing heterogeneity gives rise to alternative 5′ termini (Azuma-Mukai et al., 2008 (link); Morin et al., 2008 (link); Wu et al., 2009 (link); Chiang et al., 2010 (link)). To annotate these abundant alternative isoforms, we identified all isoforms expressed at ≥33% of the level of the most abundant isoform, as determined from high-throughput sequencing (allowing for 3′ heterogeneity within each isoform). These isoforms were carried forward as mammalian conserved isoforms if they also satisfied this property in the mouse small-RNA sequencing data (Chiang et al., 2010 (link)), and as broadly conserved isoforms if they satisfied this property in zebrafish small-RNA sequencing data available in miRBase release 21. Adhering to the miRNA naming convention, if two isoforms mapped to the 5′ and 3′ arms of the hairpin they were named ‘–5p’ and ‘–3p’, respectively, and if two isoforms were processed from the same arm they were named ‘.1’ and ‘.2’ in decreasing order of their abundance, as detected in the human.
All mature miRNAs were downloaded from miRBase release 21 (Kozomara and Griffiths-Jones, 2014 (link)). Those that matched a conserved miRNA at nucleotides 2–8 were considered part of that miRNA family. All miRNAs and miRNA isoforms annotated in miRBase but not meeting our criteria for conservation in mammals or beyond were also grouped into families based on the identity of nucleotides 2–8 and were classified as poorly conserved miRNAs (which included many small RNAs misclassified as miRNAs). The miRNA seed families and associated conservation classifications are available for download at TargetScan (targetscan.org).
Agarwal V., Bell G.W., Nam J.W, & Bartel D.P. (2015). Predicting effective microRNA target sites in mammalian mRNAs. eLife, 4, e05005.
Full text: Click here
Publication 2015
5
Optimized Particle Picking for Cryo-EM
Cited 61 times
All reconstructions were performed using cryoSPARC25 (link). For all particle picking approaches, we performed 2D Classification with default parameters and 100 2D classes, then removed obvious non-particles. For the DoG dataset, four rounds of 2D Classification yielded 770,263 particles from an initial stack of 1,599,638. For the template dataset, four rounds of 2D Classification yielded 627,533 particles from an initial stack of 1,265,564. For the Topaz dataset, one round of 2D Classification yielded 1,006,089 particles from an initial stack of 1,010,937. For the crYOLO dataset, one round of 2D Classification yielded 131,300 particles from an initial stack of 133,644. For all datasets, ab initio reconstruction was used to generate an initial model, and the structures were further refined using homogeneous refinement with C1 symmetry, followed by non-uniform refinement. All parameters were left in their default setting. Unfiltered half-maps and masks were used to calculate 3DFSCs using the public server, https://3dfsc.salk.edu.
Bepler T., Morin A., Rapp M., Brasch J., Shapiro L., Noble A.J, & Berger B. (2019). Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs. Nature methods, 16(11), 1153-1160.
Publication 2019
Microtubule-Associated Proteins Reconstructive Surgical Procedures Topaz resin

Most recents protocols related to «Morin»

1
Insomnia Severity Index Protocol

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Open the protocol to access the free full text link

Publication 2024
2
Synthesis and Characterization of Morin Oxime and Semicarbazone
A proper batch of sodium acetate (20.6 mg for preparation of 2, or 30.6 mg for yielding 3 and the respective Schiff base precursor hydroxylamine hydrochloride for preparation of 2 or semicarbazide for preparation of 3) were mixed at the same weight proportion in a flask and dissolved with MeOH (3 mL). Next, morin hydrate (30 mg) previously dissolved in MeOH (2 mL) was added to the reaction mixture, which was stirred and heated for 3 h. Next, the respective crude reaction was poured on wet ice to yield a solid that was filtered and washed consecutively with dichloromethane and methanol.
Morin oxime (2). Yellow crystals (m.p. 291–293 °C); 1H NMR δ 13.24 (1H, s), 9.50 (1H, brs) 7.38 (1H, d, J = 9.0 Hz), 6.36 (1H, d, J = 2.0 Hz), 6.25 (1H, dd, J = 9.0, 2.0 Hz), 6.16 (1H, d, J = 2.0 Hz), 6.13 (1H, d J = 2.5 Hz). 13C NMR δ 178.0, 162.8, 161.7, 160.9, 160.3, 156.3, 149.0, 140.2, 128.5, 112.8, 105.6, 104.8, 103.5, 97.2, 92.9. UV (EtOH) λmax (log ε) 210 (4.6), 265 (4.4), 390 (4.4). IR 3074, 1654, 1608, 1503, 1423, 1378, 1321, 1246, 1100. HRMS m/z 317.0579 [M]+ (calcd for C15H11NO7 317.0536).
Morin semicarbazone (3). Brown crystals (m.p. 269–270 °C); 1H NMR δ 13.41 (1H, s), 9.37 (1H, brs), 7.40 (1H, d, J = 9.0 Hz), 6.32 (1H, d, J = 2.5 Hz), 6.20 (1H, dd, J = 9.0; 2.5 Hz), 6.08 (1H, d, J = 2.5 Hz), 6.06 (1H, d, J = 2.5 Hz). 13C NMR δ 178.5, 163.1, 162.5, 161.0, 160.2, 156.3, 149.0, 141.2, 127.8, 127.8, 113.7, 105.2, 105.2, 103.5, 97.0, 92.7. UV (EtOH) λmax (log ε) 210 (4.5), 265 (4.2), 390 (4.1). IR 3335, 1651, 1608, 1574, 1505, 1378, 1308, 1224, 1173. HRMS m/z 359.0758 [M]+ (calcd for C16H13N3O7 359.0753).
García-Gil S., Rodríguez-Luna A., Ávila-Román J., Rodríguez-García G., del Río R.E., Motilva V., Gómez-Hurtado M.A, & Talero E. (2024). Photoprotective Effects of Two New Morin-Schiff Base Derivatives on UVB-Irradiated HaCaT Cells. Antioxidants, 13(1), 134.
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Publication 2024
3
Apoptosis Induction in Glioma Cells
Not available on PMC !
The induction of apoptosis by morin on C6 rat glioma cells was examined using the dual staining technique. AO and EtBr stains were used for this staining to examine the apoptotic morphological changes induced by morin on glioma cells. C6 glioma cells (3 × 10 5 )/well were seeded in the six-well culture plate and incubated at 37°C in a 5% CO 2 -supplemented incubator for 24 h. Morin (25 and 50 µM/mL) were added to the cells and incubated for 24 h. The control, untreated glioma cells, and morin-treated cells were fixed using a mixture consisting of methanol and glacial acetic acid in a 3:1 ratio for 20 min at 4°C. The fixed cells were then treated with a stain solution consisting of AO and EtBr in a 1:1 ratio. The stained cells were incubated in the dark for 30 min and then rinsed with saline. The cells were then viewed under a fluorescent microscope, and the percentage of live and apoptotic cells was calculated.
Zhu S., Yuan H., Alahmadi T.A., Almoallim H.S., & Wang C. (2024). Morin Inhibits Cell Proliferation and Induces Caspase-mediated Apoptotic Cell Death in Glioma C6 Cell Line. Pharmacognosy Magazine, 20(2), 494-504.
Publication 2024
4
Morin-induced Mitochondrial Depolarization in Glioma Cells
Not available on PMC !
The depolarization of mitochondrial membrane potential (MMP) in glioma cells upon treatment with various concentrations of morin was examined using the rhodamine 123 staining technique. The 25 and 50 µM/mL morin-treated and untreated control glioma cells were stained with the lipophilic cationic dye rhodamine 123 and incubated in the dark for 30 min. The fluorescence emission of the cells was read using a fluorescence microplate reader at an excitation of 485 nm and an emission of 530 nm. The intensity of the fluorescence was measured.
Zhu S., Yuan H., Alahmadi T.A., Almoallim H.S., & Wang C. (2024). Morin Inhibits Cell Proliferation and Induces Caspase-mediated Apoptotic Cell Death in Glioma C6 Cell Line. Pharmacognosy Magazine, 20(2), 494-504.
Publication 2024
5
Cadmium-Induced Liver Injury: Morin Intervention
Not available on PMC !
Male Sprague-Dawley rats (weighing 200-250 g, 10-12 weeks old) were purchased from the Medical Experimental Research and Application Center, Atatürk University (Erzurum, Turkey). The Atatürk University Ethical Committee approved the experimental protocol for our experiment (approval no: 2020/132).
The rats were housed under standard laboratory conditions (24 ± 1 °C, 45 ± 5% humidity, and 12-h light/dark cycle). Rats had access to a commercial pellet diet (Bayramoglu Feed and Flour Industry Trade A. C. Erzurum) and water ad libitum throughout the whole study. After 1 week of acclimatization, animals were grouped into five experimental groups (control, Cd, Morin100 + Cd, Morin200 + Cd, and Morin200) with ten rats each following the design below: Control: One milliliter of distilled water was orally given to each rat for 5 consecutive days. Cd: Rats were intraperitoneally injected (i.p.) with Cd (6.5 mg/kg) [26] for 5 consecutive days. Morin100 + Cd: Morin (100 mg/kg) was orally administered to each rat [22] followed by Cd (6.5 mg/kg, i.p.) injection after 1 h of Morin administration for 5 consecutive days. Morin200 + Cd: Morin (200 mg/kg) was orally administered to each rat [22] followed by Cd (6.5 mg/kg, i.p.) injection after 1 h of Morin administration for 5 consecutive days. Morin200: Morin (200 mg/kg) was orally administered to each rat for 5 consecutive days.
On the sixth day of the experiment, live animals were weighed and then euthanized by mild sevoflurane anesthesia, and intracardiac blood samples were collected. All rats were sacrificed immediately after blood sampling by decapitation, and abdominal laparotomy was performed to obtain the liver. After weighing, each liver was divided into two parts: the 1st was snap frozen in liquid nitrogen and stored at -80 °C to be used for biochemical and gene expression studies, and the 2nd was immediately flushed with saline and then taken into 10% formaldehyde for histopathological and immunofluorescent examinations.
Sengul E., Yildirim S., Cinar İ., Tekin S., Dag Y., Bolat M., Gok M, & Warda M. (2024). Mitigation of Acute Hepatotoxicity Induced by Cadmium Through Morin: Modulation of Oxidative and Pro-apoptotic Endoplasmic Reticulum Stress and Inflammatory Responses in Rats. Biological trace element research.
Publication 2024

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1
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Rutin is a laboratory reagent used for analytical and research purposes. It is a flavonoid compound derived from various plant sources. Rutin exhibits antioxidant and anti-inflammatory properties, and is commonly used in assays, chromatography, and other analytical techniques.
7
Morin hydrate by Merck Group
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Morin hydrate is a chemical compound used as a laboratory reagent. It is a yellow crystalline solid that is soluble in water and organic solvents. Morin hydrate is commonly used as a fluorescent indicator and as a chelating agent in analytical chemistry applications.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
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More about "Morin"

Morin is a flavonoid compound found in various plants, including mulberry, onions, and guava.
It is also known as 3,5,7,2',4'-Pentahydroxyflavone and is structurally similar to the well-known flavonoids quercetin and rutin.
Morin has been extensively studied for its potential antioxidant, anti-inflammatory, and neuroprotective properties, making it a subject of interest in the fields of neurodegenerative diseases, cancer, and metabolic disorders.
Researchers can leverage AI-powered tools like PubCompare.ai to streamline the identification and comparison of Morin-related protocols from the literature, preprints, and patents.
This platform allows for efficient and reproducible research by facilitating the discovery of relevant protocols and enabling AI-driven comparisons to identify the best protocols and products for specific research needs.
To further explore the potential of Morin, researchers may utilize a variety of experimental techniques and tools, such as FBS (Fetal Bovine Serum) for cell culture, GraphPad Prism 5 for data analysis, and kits like the RNeasy Mini Kit and TRIzol for RNA extraction and purification.
Additionally, compounds like quercetin, rutin, and Morin hydrate may be used as reference standards or for comparative studies.
The incorporation of Penicillin/streptomycin, Lipofectamine 2000, and other reagents and tools can also be valuable in Morin-related research, depending on the specific experimental design and objectives.
By leveraging these resources and techniques, researchers can further elucidate the mechanisms of action and potential therapeutic applications of Morin, ultimately contributing to the advancement of scientific knowledge and the development of novel therapeutic strategies.
With the aid of PubCompare.ai and other innovative tools, the exploration of Morin and its effects continues to evolve, offering promising avenues for future research and clinical applications.