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N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate

N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate is a chemical compound with a complex structure that plays a role in various biological processes.
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Most cited protocols related to «N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate»

1
Isolation and Identification of Vaginal Lactobacilli
Cited 16 times
Fifteen pre-menopausal Caucasian women (aged 18–45 years old), who have no symptoms of vaginal or urinary tract infection, were recruited for the present study. The women were non-menstruating and not receiving oral or local antimicrobial therapy within the previous 2 weeks. All volunteers provided a written informed consent in accordance with the Ethics Committee of the University of Bologna (52/2014/U/Tess) and the institutional review board approved the study. Mid-vaginal secretions were self-collected by women with E-swabs (Copan, Brescia, Italy) and immediately processed for lactobacilli isolation. The specimens were coded to assure full anonymousness.
Lactobacillus clones were isolated onto de Man, Rogosa and Sharpe (MRS) and Brain-Heart Infusion (BHI) agar plates (Difco, Detroit, MI). Both MRS and BHI agar plates were supplemented with 0.05% L-cysteine. Plates were incubated anaerobically for 24 h at 37°C in anaerobic jars supplemented with Anaerocult C (Merck, Milan, Italy). Colonies with different morphologies yielding variable rods by microscope observation were selected for glycerol stock preparation. To prepare lactobacilli fractions, 18-h MRS/BHI cultures (OD600 = 0.5) were centrifuged at 5,000 X g for 10 min at 4°C. Supernatants were filtered through a 0.2 μm membrane filter to obtain cell free supernatants (CFS). Cell pellets (CP) were washed in sterile saline.
Genomic DNA was extracted from lactobacilli CP using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) following the protocol “Pretreatment for Gram-positive bacteria”. The extracted DNA was amplified with Lactobacillus genus-specific primers Lac1 and Lac2 [32 (link)]. The positive isolates were taxonomically characterized to the species level by sequencing the 16S ribosomal RNA (rRNA) gene. Briefly, the complete 16S rRNA gene (1.5 kb) was amplified with the universal primers 27F and 1492R [33 ] and sequenced. The obtained sequences were compared with the sequences available in the Ribosomal Database Project (RDP, http://rdp.cme.msu.edu/) [34 (link)] in order to identify the Lactobacillus species.
Parolin C., Marangoni A., Laghi L., Foschi C., Ñahui Palomino R.A., Calonghi N., Cevenini R, & Vitali B. (2015). Isolation of Vaginal Lactobacilli and Characterization of Anti-Candida Activity. PLoS ONE, 10(6), e0131220.
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Publication 2015
Agar Blood Brain Caucasoid Races Cells Clone Cells Cysteine Ethics Committees Ethics Committees, Research Genome Glycerin Gram-Positive Bacteria Heart isolation Lactobacillus Microbicides Microscopy N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate Oligonucleotide Primers Pellets, Drug Premenopause Ribosomal RNA Genes Ribosomes Rod Photoreceptors Saline Solution Secretions, Bodily Sterility, Reproductive Therapeutics Tissue, Membrane Tissues Urinary Tract Infection Vagina Voluntary Workers Woman
2
Evaluating Landscape Genetic Models
Cited 13 times
To enable comparisons with other models, we simulated genotypic data from spatial coalescent models with the computer program ms (Hudson 2002 (link)). Ten data sets were generated according to a linear stepping-stone model with 40 demes, setting the effective migration rate between pairs of adjacent demes to the value . Sampling five individuals in each deme, each data set included a total of n = 200 haploid individuals genotyped at unlinked SNP loci. We ran the LFMM during 100 sweeps for burn-in, and we used the next 900 sweeps to compute point estimates, variances, and -scores. An environmental variable, x, was defined for each population as the geographic identifier of the population in the linear stepping-stone model.
We created an environmental gradient for the artificial variable x using a logistic function, s(x), of x as follows

For each of the 10 previously generated neutral stepping-stone simulations, we simulated binary alleles at 50 unlinked loci for each deme x with frequency s(x), and with the slope of the gradient . We then obtained 10 data sets with unlinked loci including 50 loci correlated with the environmental gradient, s(x). Using Tracy–Widom tests implemented in SmartPCA, we found that the number of principal components with P values smaller than 0.01 was around . Using the Bayesian clustering programs STRUCTURE and TESS, we found that components could better describe our simulated data. A value corresponds to a strong intensity of selection through geographic space, whereas corresponds to a weak intensity of selection. We used the value when comparing tests based on linear and PC regression models. When comparing LFMMs with BAYENV, we used the value to better fit the objectives of both models. As BAYENV returns Bayes factors instead of P values, we considered ranked lists recording the M loci corresponding to the strongest associations (M between 1 and ). For each M, we computed the number of TPs and the number of FPs. Locus ranking was performed on the basis of -scores in LFMM and on the basis of Bayes factors in BAYENV. The LFMM tests used values of K equal to and 20, and we used of the BAYENV algorithm to compute Bayes factors. Experiments were assessed by counting the number of FP and FN associations, and by measuring the AUC averaged over 10 replicates.
Frichot E., Schoville S.D., Bouchard G, & François O. (2013). Testing for Associations between Loci and Environmental Gradients Using Latent Factor Mixed Models. Molecular Biology and Evolution, 30(7), 1687-1699.
Publication 2013
2,2-dichloro-1,1-difluoroethyl difluoromethyl ether Alleles Calculi Debility Minor Lymphocyte Stimulatory Loci N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate Properdin
3
Assessing Community Stress and Health Impacts
Cited 9 times
TESS reflects a mixed methods survey of two large adjacent neighborhoods in response to community-driven health and social justice concerns. The standardized English and Spanish instruments were developed by an interdisciplinary team that consisted of community representatives and scholars with a background in psychology, sociology, anthropology, history and/or health professions. Questions from reliable and validated surveys used by the Centers for Disease Control and Prevention’s Behavioral Risk Factor Surveillance System (BRFSS) Survey, previous and new measures of culturally-specific stressors, acculturation and ethnic identity, and prior mistreatment surveys conducted in a nearby community11 ,12 were employed. Items were selected from the interdisciplinary team to match community and research goals while not overburdening participants. Translated items were available in most instances; multiple native Spanish speakers were involved in any required translations and reviewed all previous Spanish translations (and made adjustments) for appropriateness to current and local community usage.
This survey’s closed ended, open ended, and narrative questions were asked by trained bilingual interview pairs in houses, apartments, libraries, churches and community centers. A convenience, cross-sectional sample of 174 adults was obtained. Overall response rate could not be determined because the total number of all persons who became aware of the announcement of the survey opportunity was not determined. Anecdotally we believe the participation rate was about 40%, with interviewers perceiving palpable concerns among community members to participate in an official appearing survey. Interviewers were instructed to be inclusive in recruitment toward all community residents, participant refusals once the survey process began were minimal.
All interview pairs included highly fluent Spanish and English speakers within them, though not necessarily a native Spanish speaker. All human subjects protocols were approved by the overseeing institution’s federally approved internal review board and the project complied with American Psychological Associations ethical guidelines.
Stress was measured with the previously described 21item BCISS scale (items are presented in Table 1). The following were notes for the interviewers prior to administering the scale: “Within the last 3 months, has/have (each item, e.g., the need to speak better Spanish) made you_______ not all stressful, a little stressful, moderately stressful, very stressful, or extremely stressful, or have you not experienced stress due to this situation in the last 3 months, or have you never experienced stress over it?”/ “Dentro de los ultimos 3 meses [la situacion; por ejemplo, “la necesidad de hablar mejor espanol”] le he causado que no siente estres, que sienta un poco de estres, que siente un estres moderado, que sienta mucho estres, que sienta demasiado estres, o que no ha sentido estres por dicha situacion durante los ultimos tres meses, o que nunca le ha causado estres dicha situacion.” The interviewers were trained to note whether the stressor was ever experienced, experienced in the last 3 months, and for those who experienced the stressor in the last 3 months, the intensity of the stressor in a 5-point Likert format (i.e., not at all stressful/ le he causado que no siente estres to extremely stressful/ que sienta demasiado estres). The current study focused on summation of intensely reported stressors, those reported in the last two categories (very and extremely stressful), and total stress using a quantitative stress index considering the presence and intensity of stressors.7 (link),21 (link) When the items were represented by presence/intensity the scale showed high internal consistency (α= 0.91) and had a range of 0–58 (M = 14.3; SD = 14.6; Mdn = 10). As a follow up after the scale was completed, an open-ended question asked “Do you have other worries or sources of stress that you want to talk about?”/”¿Le gustaría hablar de otras preocupaciones o estresantes que pueda tener?”.
Other variables from the TESS study and selected for current study are reports of general health (an established predictor of objectively measured health markers), physical health, mental health, and depressive symptoms. Depressive symptoms were measured with a five-item shortened version of the CES-D.22 The measure included assessments of the frequency of experiencing each of the four major symptom domains within the past week. Depressive symptoms was kept continuous6 (link), internal consistency of the scale was adequate (α =0.79).
Descriptive analysis included reported frequencies and percentages of each item. Bivariate correlations were used to express the strength of association, direction of influence and statistical significance between total stress and health outcomes; these were conducted in the full sample as well as stratified by gender and by place of birth. Analysis of variance and multiple linear regressions were used to test for mean differences in gender and place of birth in total stress as well as their potential operation as effect modifiers in explaining the health outcomes.
Carvajal S.C., Rosales C., Rubio-Goldsmith R., Sabo S., Ingram M., McClelland D.J., Redondo F., Torres E., Romero A.J., Oleary A.O., Sanchez Z, & de Zapien J.G. (2013). The Border Community & Immigration Stress Scale: A Preliminary Examination of a Community Responsive Measure in Two Southwest Samples. Journal of immigrant and minority health / Center for Minority Public Health, 15(2), 427-436.
Publication 2013
Adult Behavioral Risk Factor Surveillance System Depressive Symptoms Gender Hispanic or Latino Homo sapiens Inclusion Bodies Interviewers Mental Health N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate Physical Examination Speech
4
Vitamin A Regulation of RAR-Mediated Transcription
Cited 8 times
Mice were maintained at IMM or VU University Medical Centre according to national and international guidelines. Bone marrow cells were isolated from 8 week-old C57Bl/6 CD45.1 mice and injected intravenously into 2 week-old lethally irradiated CD45.2 ROSA26-RARa403Het (WT→RarHet) or WT littermate controls (WT→WT). C57Bl/6 female mice received either vitamin A deficient (VAD), with no vitamin A (vitamin free casein), vitamin A high (VAH, 25,000IU/Kg) or vitamin A control (VAC, 4,000IU/kg) diets. Retinoic acid was provided to pregnant mice from E10.5 until sacrifice. Quantitative real time RT-PCR was performed as previously described6 (link),7 (link),10 (link). Computational analysis was performed with TESS. DNA/protein complexes were immunoprecipitated using antibodies against mouse pan-RAR, pan-RXR or control IgG.
van de Pavert S.A., Ferreira M., Domingues R.G., Ribeiro H., Molenaar R., Moreira-Santos L., Almeida F.F., Ibiza S., Barbosa I., Goverse G., Labão-Almeida C., Godinho-Silva C., Konijn T., Schooneman D., O’Toole T., Mizee M.R., Habani Y., Haak E., Santori F.R., Littman D.R., Schulte-Merker S., Dzierzak E., Simas J.P., Mebius R.E, & Veiga-Fernandes H. (2014). Maternal retinoids control type 3 innate lymphoid cells and set the offspring immunity. Nature, 508(7494), 123-127.
Publication 2014
Antibodies Bone Marrow Cells Caseins Diet Females Mice, Inbred C57BL Mus N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate Proteins Real-Time Polymerase Chain Reaction Tretinoin Vitamin A Vitamins
5
Placental Gene Regulation and Trophoblast Function
Cited 7 times

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Publication 2014
5' Untranslated Regions Anti-Antibodies Antibodies Binding Sites Biological Assay Biological Evolution Biopharmaceuticals CEBPB protein, human Cells DNA Chips DNA Transposable Elements Exons Formalin Freezing Galactose Binding Lectin Genes Genome Homo sapiens hydrogen sulfite Immunoprecipitation In Situ Hybridization isolation Laser Capture Microdissection LGALS13 protein, human Luciferases Methylation Microarray Analysis N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate Paraffin Placenta Pseudogenes Specimen Collection Tissues Transcription, Genetic Transcription Factor Trees Trophoblast Umbilical Cord Blood Untranslated Regions Woman

Most recents protocols related to «N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate»

1
SETD2 Proficiency in Mast Cell Disorders
Experiments were conducted in SETD2-proficient (ROSAKIT D816V) and -deficient (HMC-1) cell lines [17 (link)–19 (link)] and in primary cells from patients with various SM subtypes. Cell lines and patient characteristics are detailed in the Supplementary methods. Sample collection was approved by the Institutional Review Boards of the S. Orsola-Malpighi Hospital (protocol 112/2014/U/Tess) and of the other institutions participating in this study.
Mancini M., Monaldi C., De Santis S., Papayannidis C., Rondoni M., Sartor C., Bruno S., Pagano L., Criscuolo M., Zanotti R., Bonifacio M., Tosi P., Arock M., Valent P., Cavo M, & Soverini S. (2023). SETD2 non genomic loss of function in advanced systemic mastocytosis is mediated by an Aurora kinase A/MDM2 axis and can be therapeutically targeted. Biomarker Research, 11, 29.
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Publication 2023
Cell Lines Cells Ethics Committees, Research hSet2 protein, human N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate Patients Specimen Collection
2
Isolation and Characterization of Vaginal Lactobacilli
Cited 1 time
Lactobacilli included in this study were previously isolated from vaginal swabs of healthy pre-menopause Caucasian women, according to the protocol approved by the Ethics Committee of the University of Bologna, Bologna, Italy (52/2014/U/Tess) [47 (link)]. According to a recent reclassification of Lactobacillus genus [48 (link)], they belong to the species Lactobacillus crispatus (BC1, BC3, BC4, and BC5), Lactobacillus gasseri (BC9, BC10, BC12, and BC14) and Limosilactobacillus vaginalis (BC16 and BC17). Lactobacilli were routinely grown in de Man, Rogosa, and Sharpe broth (MRS) (Difco, Detroit, MI, USA) with the addition of L-cysteine 0.05% (w/v) (Merck, Milan, Italy), at 37 °C; the anaerobic conditions were guaranteed by incubation in anaerobic jars containing Gas-Pak EZ (Beckton, Dickinson and Co., Milan, Italy).
Escherichia coli DSM1900, E. coli DSM18039 and Staphylococcus aureus DSM2569 were purchased from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ, Braunschweig, Germany). Staphylococcus lugdunensis BC102, Enterococcus faecalis BC101 and Enterococcus faecium BC105 belong to the Department of Pharmacy and Biotechnology of the University of Bologna (Italy). Staphylococcus aureus SO88, Streptococcus agalactiae SO104, Candida albicans SO1 and Candida glabrata SO17 were isolated at Sant’Orsola-Malpighi University Hospital of Bologna during routine diagnostic procedures. The microbial identification was obtained by means of a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), using a Bruker Microflex MALDI-TOF MS instrument (Bruker Daltonics) [49 (link)]. Staphylococcus spp., E. coli, Enterococcus spp. and S. agalactiae were aerobically grown at 37 °C in Brain Heart Infusion medium (BHI) (Difco, Detroit, MI, USA), while Candida spp. were aerobically cultured at 35 °C in Sabouraud dextrose medium (SD) (Difco, Detroit, MI, USA).
Giordani B., Naldi M., Croatti V., Parolin C., Erdoğan Ü., Bartolini M, & Vitali B. (2023). Exopolysaccharides from vaginal lactobacilli modulate microbial biofilms. Microbial Cell Factories, 22, 45.
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Publication 2023
BC-105 Brain Candida Candida albicans Candida glabrata Caucasoid Races Cell Culture Techniques Culture Media Cysteine Diagnostic Tests, Routine Enterococcus Enterococcus faecalis Enterococcus faecium Escherichia coli Ethics Committees Glucose Heart Lactobacillus Lactobacillus crispatus Lactobacillus gasseri Lactobacillus vaginalis N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate Premenopause Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Staphylococcus Staphylococcus aureus Staphylococcus lugdunensis Streptococcus agalactiae Vagina Woman
3
Isolation and Expansion of Human Wharton's Jelly Mesenchymal Stem Cells
Human WJ-MSCs were isolated from umbilical cords of 3 healthy donor mothers after informed consent as previously described [67 (link)]. The study was approved by the Local Ethical Committee of Bologna (IRCCS St. Orsola-Malpighi University Hospital Ethical Committee, protocol n° 2481/2017, ref n° 68/2017/U/Tess). Cells were cultured in Dulbecco’s Modified Eagle’s Medium—1 g/L of glucose (L-DMEM; Corning Incorporated, Corning, NY, USA) supplemented with 10% Fetal Bovine Serum (FBS; Gibco, Waltham, MA, USA) and antibiotics (1% Penicillin-Streptomycin Solution; Thermo-Fisher Scientific, Waltham, MA, USA) and were maintained in standard culture conditions at 37 °C with 5% carbon dioxide (CO2) in a humidified atmosphere. The non-adherent cells were removed, and medium was changed twice a week. When cells reached 80% confluence, they were detached using a trypsin-EDTA solution (Sigma-Aldrich Co., St. Louis, MO, USA) and were seeded for maintenance and/or expansion. For experiments, cells were seeded at a density of 5000 cells/cm2 (except where otherwise noted) in adequate plastic support (Corning Incorporated, Corning, NY, USA) and incubated in standard condition for 24 h before treatment. Experiments were performed using hWJ-MSCs at the 3rd–6th culture passages and were repeated in biological triplicate (n = 3).
Pampanella L., Abruzzo P.M., Tassinari R., Alessandrini A., Petrocelli G., Ragazzini G., Cavallini C., Pizzuti V., Collura N., Canaider S., Facchin F, & Ventura C. (2023). Cytochalasin B Influences Cytoskeletal Organization and Osteogenic Potential of Human Wharton’s Jelly Mesenchymal Stem Cells. Pharmaceuticals, 16(2), 289.
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Publication 2023
Antibiotics Atmosphere Biopharmaceuticals Carbon dioxide Cells Eagle Edetic Acid Glucose Homo sapiens Mothers N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate Penicillins Streptomycin Tissue Donors Trypsin Umbilical Cord
4
Serum miRNA Profile in Liver Transplant Recipients
The enrolment of patients was conducted following the protocol of donor–recipient allografts performed at the Hepatobiliary and Transplant Surgery Unit (IRCCS, S. Orsola Hospital, Bologna, Italy) with the ethical committee’s approval (code: 44/2008/Tess) in the framework of a national project (PRIN2008), several years before the SARS-CoV-2 pandemic period. Serum samples were collected from 14 LT recipients before surgery (pre-LT) and during the follow-up period (FU) at different times after transplantation, for a total of 28 samples. The main characteristics of this group of patients are reported in Table 1.
The second group of samples was collected from recipients only after LT in the FU period (N = 19). The main characteristics of this cohort are summarized in Table 2. The same serum samples, for both cohorts, were analyzed and described in detail in the previous publication [11 (link)], even if some serum samples from those patients are currently no longer available. Thus, the total number of enrolled recipients was 33, their age range was from 25 up to 69 years, and their average age was of 50.6 ± 10.5 (years ± SD).
Furthermore, serum samples collected from a nontransplanted control group (N = 24) were evaluated as a reference for all of the investigated miRs. The control group was constituted by 28–68 year old healthy subjects, average 49.3 ± 12.3 (years ± SD), matching the age and sex of the recipients.
Liver function markers were acquired for all patients enrolled in the study, in collaboration with the General Surgery and Transplant Unit (S. Orsola Hospital, Bologna, Italy). In particular, total bilirubin (TBIL), indirect bilirubin (IBIL), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), alpha-fetoprotein (ALP), and albumin (ALB) were acquired. These data were also described in a previous study [11 (link)].
Morsiani C., Collura S., Sevini F., Ciurca E., Bertuzzo V.R., Franceschi C., Grazi G.L., Cescon M, & Capri M. (2023). Circulating miR-122-5p, miR-92a-3p, and miR-18a-5p as Potential Biomarkers in Human Liver Transplantation Follow-Up. International Journal of Molecular Sciences, 24(4), 3457.
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Publication 2023
Albumins Allografts alpha-Fetoproteins Bilirubin D-Alanine Transaminase Donors gamma-Glutamyl Transpeptidase Grafts Healthy Volunteers Liver N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate Operative Surgical Procedures Pandemics Patients SARS-CoV-2 Serum Transaminase, Serum Glutamic-Oxaloacetic Transplantation
5
Placental Cell Isolation Protocol
This study was approved by the Local Ethical Committee (IRCCS St. Orsola-Malpighi University Hospital Ethical Committee, protocol n° 2481/2017, ref n° 68/2017/U/Tess). Placentas were obtained from healthy donor mothers undergoing elective caesarean section at term (37–40 weeks) after written informed consent. Perinatal tissues were maintained under sterile conditions until specific cell isolation was performed.
Paris F., Marrazzo P., Pizzuti V., Marchionni C., Rossi M., Michelotti M., Petrovic B., Ciani E., Simonazzi G., Pession A., Bonsi L, & Alviano F. (2023). Characterization of Perinatal Stem Cell Spheroids for the Development of Cell Therapy Strategy. Bioengineering, 10(2), 189.
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Publication 2023
Cell Separation Cesarean Section N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate Placenta Sterility, Reproductive Tissue Donors Tissues

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More about "N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate"

N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate, also known as THAM or Tris buffer, is a chemical compound with a complex structure that plays a crucial role in various biological processes.
This versatile compound is widely used in biochemistry, cell biology, and molecular biology research.
Researchers often work with N-tris(hydroxymethyl)methyl-2-aminomethane sulfonate to maintain pH and buffer solutions, as it acts as a pH-stabilizing agent.
It is commonly used in conjunction with other laboratory reagents, such as FBS (Fetal Bovine Serum), L-cysteine, Penicillin/streptomycin solution, RNeasy Mini Kit, RPMI 1640 medium, and TurboFect transfection reagent.
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