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Necrostatin-1

Q: Are there different variations or types of Necrostatin-1?

Yes, there are several variations of Necrostatin-1 that researchers can use. These include Necrostatin-1s, a more soluble analogue, as well as Necrostatin-5, which has been reported to have improved specificity for RIPK1. The choice of Necrostatin-1 variation may depend on the specific research goals and the characteristics of the experiment.

Necrostatin-1 is a small-molecule inhibitor of necroptosis, a form of programmed cell death.
It has been widely studied for its potential therapeutic applications in various diseases, including neurodegeneration, ischemia-reperfusion injury, and inflammatory disorders.
Necrostatin-1 acts by targeting the receptor-interacting protein kinase 1 (RIPK1), a key mediator of necroptosis, and has been shown to be effective in preclinical models in preventing cell death and reducing tissue damage.
Researchers can leverage PubCompare.ai's AI-driven platform to easily identify the most reproducibble and accurate protocols for working with Necrostatin-1, as well as the best products to enhance their research outcomes.

Most cited protocols related to «Necrostatin-1»

Maintenance and Differentiation of Cell Lines

Cited 26 times

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Publication 2014

benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone Biological Factors BMS345541 Bone Marrow Cells CASP8 protein, human Culture Media, Conditioned Cycloheximide Femur Fetal Bovine Serum Glucose Glutamine HT29 Cells Macrophage Colony-Stimulating Factor necrostatin-1 Neutrophil NIH 3T3 Cells Penicillins Poly I-C Promega quinoline-val-asp(OMe)-CH2-OPH RNA, Small Interfering Streptomycin Sulfoxide, Dimethyl SYTOX Green Tibia Tumor Necrosis Factor-alpha

Caspase-8 Depletion in Intestinal Epithelial Cells

Cited 11 times

Mice carrying a loxP-flanked caspase-8 allele (Casp8^fl) and Villin-Cre mice were described earlier^{27 (link)-29 (link)}. IEC-specific caspase-8 knockout mice were generated by breeding Casp8^fl mice to Villin-Cre or Villin-CreERT2 mice. Experimental colitis was induced with 1-1.5% dextrane sodium sulfate (DSS, MP Biomedicals) in the drinking water for 5-10 day. Colitis development was monitored by analysis of weight, rectal bleeding and colonoscopy as previously described^{30 (link)}. In some experiments, mice were injected intravenously with TNF-α (200 ng/g body weight, Immunotools) plus/minus necrostatin-1 (1.65 μg/g body weight, Enzo). Histopathological analysis was performed on formalin-fixed paraffin embedded tissue after H&E staining. Immunofluorescence of cryosections was performed using the TSA-Kit as recommended by the manufacturer (PerkinElmer). For electron microscopy, glutaraldehyde fixed material was used. Ultrathin sections were cut and analyzed using a Zeiss EM 906 (Zeiss). Paraffin-embedded patient specimens were obtained from the Institute of Pathology and endoscopic biopsies were collected in the Department of Medicine 1 (Erlangen University). The collection of samples was approved by the local ethical committees and each patient gave written informed consent. For organoid culture, intestinal crypts were isolated from mice and cultured as previously described^{11 (link)}. Organoid growth was monitored by light microscopy. IEC were isolated as previously described^{5 (link)}. For gene chip experiments, total RNA of IEC was isolated using the RNeasy Mini Kit (Qiagen) and hybridised to the Affymetrix mouse 430 2.0 chip (Affymetrix, Santa Clara, CA). Gene ontology based analyses were performed using the online tool Database for Annotation, Visualization and Integrated Discovery (DAVID). Caspase-3/-7 activity was measured using the Caspase-Glo3/7 Assay (Promega) according to the manufacturer's instructions.

Günther C., Martini E., Wittkopf N., Amann K., Weigmann B., Neumann H., Waldner M., Hedrick S.M., Tenzer S., Neurath M.F, & Becker C. (2011). Caspase-8 regulates TNF-alpha induced epithelial necroptosis and terminal ileitis. Nature, 477(7364), 335-339.

Publication 2011

Alleles Biological Assay Biopsy Body Weight Caspase-7 Caspase-8 CFC1 protein, human Colitis Colonoscopy Cryoultramicrotomy DNA Chips Electron Microscopy Endoscopy Formalin Gene Chips Glutaral Immunofluorescence Intestines Light Microscopy Mice, Knockout Mus necrostatin-1 Organoids Paraffin Paraffin Embedding Patients Pharmaceutical Preparations Promega sodium sulfate Specimen Collection Tumor Necrosis Factor-alpha villin

Caspase-8 Depletion in Intestinal Epithelial Cells

Cited 11 times

Publication 2011

Characterization of L929sAhFas Cell Line

Cited 5 times

L929sAhFas cells were generated as previously described^{47 (link)} and are referred to as L929sA cells for simplicity. Cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum, penicillin (100 IU/ml), streptomycin (0.1 mg/ml) and ℒ-glutamine (0.03%). The recombinant mouse TNF used for in vivo experiments and human TNF for in vitro experiments were produced in Escherichia coli and purified in our laboratories. The activity and endotoxin contamination of mTNF, determined by MTT assay and the Limulus amebocyte lysate assay (Kabivitrum, Trenton, NJ, USA), was 1.7 × 10⁸ IU/mg and 0.47 EU/mg, respectively. Activity of human TNF used in vitro was 3.3 × 10⁸IU/mg. The 5-diphenyltetrazolium bromide (MTT; Sigma Aldrich, St. Louis, MO, USA) was used at 500 mg/ml. Nec-1 (full name, 5-((1H-indol-3-yl)methyl)-3-methyl-2-thioxoimidazolidin-4-one) and Nec-1i (full name 5-((1H-indol-3-yl)methyl)-2-thioxoimidazolidin-4-one) were purchased from Calbiochem (San Diego, CA, USA). Nec-1s (full name, 5-((7-Cl-1H-indol-3-yl)methyl)-3-methylimidazolidine-2,4-dione) was prepared and kindly provided by Brigham and Women's Hospital, Inc, Boston. See website for structures and nomenclature (http://www.nature.com/nchembio/journal/v1/n2/compound/nchembio711_ci.html). 1-MT (Sigma Aldrich) was dissolved in PBS.

Takahashi N., Duprez L., Grootjans S., Cauwels A., Nerinckx W., DuHadaway J.B., Goossens V., Roelandt R., Van Hauwermeiren F., Libert C., Declercq W., Callewaert N., Prendergast G.C., Degterev A., Yuan J, & Vandenabeele P. (2012). Necrostatin-1 analogues: critical issues on the specificity, activity and in vivo use in experimental disease models. Cell Death & Disease, 3(11), e437-.

Publication 2012

Biological Assay Bromides Cells Culture Media endotoxin binding proteins Endotoxins Escherichia coli Fetal Bovine Serum Glutamine Homo sapiens Kabivitrum Limulus Mus PCSK1 protein, human Penicillins Streptomycin

Multiplex Cytokine Assay for Radiation Damage

Cited 4 times

A list of the 33 proteins assayed and the known primary function of each is shown in Table 1. Selection of these 33 proteins was based on the pathways involved in the known inflammatory and radiation-induced damage responses summarized in Table 2. A Bio-Rad protein assay was run for each tissue type (plasma, intestine, bone marrow) and experimental group [9.25 Gy irradiation only (control); 9.25 Gy with JP4-039; 9.25 Gy with necrostatin-1; and 9.25 Gy with JP4-039 at 24 h and necrostatin-1 at 72 h after TBI, as well as other delivery times for the 2 drugs] using a Biotek® Epoch™ Microplate Spectrophotometer (BioTek Instruments Inc., Winooski, VT). We utilized the TGF β1 Single Plex Magnetic Bead Kit, as well as the 32-Multiplex Mouse Cytokine/Chemokine Magnetic Bead Panel (EMD Millipore, Billerica, MA) that tested protein concentrations for eotaxin, G-CSF, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-2, regulated upon activation normal T cell expressed and secreted (RANTES), TNF-α and VEGF.
Each kit (MCYTOMAG-70K and TGFBMAG-64K-01) provided reagents for use in the Luminex Protein Immunoassay, which included one vial of mouse cytokine/chemokine standard, two vials mouse cytokine/chemokine quality controls, one vial serum matrix, 96-well plate, 30 ml assay buffer, 60 ml 10× wash buffer, one 3.2-ml bottle of mouse cytokine detection antibodies and one 3.2-ml bottle of streptavidin-phycoerythrin. The 32-Multiplex Kit (MCYTOMAG-70K) provided one 3.5-ml pre-mixed 32-plex beads. The TGF β1 kit (TGFBMAG-64K-01) provided the above reagents, as well as 5 ml sample diluent, 1.0 ml 1.0 N HCl, 1.0 ml 1 N NaOH and 3.5 ml anti-TGF-β1 beads.

Steinman J., Epperly M., Hou W., Willis J., Wang H., Fisher R., Liu B., Bahar I., McCaw T., Kagan V., Bayir H., Yu J., Wipf P., Li S., Huq M.S, & Greenberger J.S. (2017). Improved Total-Body Irradiation Survival by Delivery of Two Radiation Mitigators that Target Distinct Cell Death Pathways. Radiation research, 189(1), 68-83.

Publication 2017

Most recents protocols related to «Necrostatin-1»

Compound Screening for Cell Death

Elesclomol (STA-4783), Puromycin (CL13900), Ferrostain-1 (S7243), and Tetrathiomolybdate (E1166) were purchased from Selleck Chemicals (China). Z-VAD-FMK (HY-16658B), N-Acetylcysteine (HY-B0215) and Necrostatin-1 (HY-15760) were purchased from MedChemExpress (China).

Liu C., Chen L., Cong Y., Cheng L., Shuai Y., Lv F., Chen K., Song Y, & Xing Y. (2024). Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers. Cell Death & Disease, 15(2), 149.

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Publication 2024

Pharmacological Modulation of Cell Death

All the inhibitors and inducers were purchased from Selleck Chemicals (USA). The Ferrostatin-1 (Ferr-1) with 5 μM, DFO with 50 μM, 3-Methyladenine (3-MA) with 5 mM, Bafilomycin A1 (BafA1) with 50 nM, Z-VAD-FMK (Z-VAD) with 10 μM, Necrostatin-1 (Nec-1) with 2 μM, Erastin with 10 μM stimulated the indicated cells for 48 h before detection, respectively.

Chen L., Lin W., Zhang H., Geng S., Le Z., Wan F., Huang Q., Chen H., Liu X., Lu J.J, & Kong L. (2024). TRIB3 promotes malignancy of head and neck squamous cell carcinoma via inhibiting ferroptosis. Cell Death & Disease, 15(3), 178.

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Publication 2024

Arnicolide D Cytotoxicity Evaluation

Arnicolide D (C₁₉H₂₄O₅, CAS34532-68-8) was purchased from Jiangsu Yongjian Pharmaceutical Co., Ltd (Jiangsu, China). Chemical reagents, including N-AcetylL-cysteine (NAC), propidium iodide (PI), z-VAD-fmk, ferrostatin-1, necrostatin-1, cycloheximide (CHX), and GSK2656157, were obtained from MedChemExpress (Shanghai, China). Dulbecco’s Modified Eagle’s Medium, fetal bovine serum, and Lipofectamine™ 3000 were sourced from Thermo Fisher (MA, USA).

Lin Y.S., Sun Z., Shen L.S., Gong R.H., Chen J.W., Xu Y., Yu H., Chen S, & Chen G.Q. (2024). Arnicolide D induces endoplasmic reticulum stress-mediated oncosis via ATF4 and CHOP in hepatocellular carcinoma cells. Cell Death Discovery, 10, 134.

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Publication 2024

Evaluating Cell Death Pathways

All-trans-retinal (atRAL) (TRC, Canada), 4-Hydroxynonenal (4-HNE) (Sigma, Burlington, MA), 2-(4-Amino-3-methylphenyl)benzothiazole (DF 203) (eMolecules, San Diego, CA), Z-VAD (R&D, Minneapolis, MN), Necrostatin-1 (Biovision, Waltham, MA), 5,11-Dihydroindolo[3,2-b]carbazole-6-carboxaldehyde (FICZ), and kynurenic acid (Tocris, Minneapolis, MN) were used in this study. atRAL was stored and manipulated with limited light exposure.

Schustak J., Han H., Bond K., Huang Q., Saint-Geniez M, & Bao Y. (2024). Phenotypic high-throughput screening identifies aryl hydrocarbon receptor agonism as common inhibitor of toxin-induced retinal pigment epithelium cell death. PLOS ONE, 19(4), e0301239.

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Publication 2024

Comprehensive Reagent Toolkit for Cell Death Studies

The following drugs were used: Z-VAD-fmk (Promega, G7231), necrostatin-1 (Sigma, N9037), liproxstatin-1 (Sigma, SML1414), Fer-1 (Sigma, SML0583), N-acetyl-l-cysteine (Sigma, A7250), MitoTEMPO (Sigma, SML0737), deferoxamine mesylate salt (Merck, D9533), staurosporine (Sigma, S5921), erastin (Sigma, E7781), 1S,3R-RSL3 (Sigma, SML2234), iFSP1 (Sigma, SML2749), arachidonic acid (Sigma, 10931), FIN56 (Sigma, SML1740), sorafenib (Santa Cruz, sc-220125), inPISD inhibitor (or STK770988; Vitasmlab.biz), mitoquinol (Cayman Chemicals, 89950), CCCP (Sigma, C2759) and ULK1 inhibitor SBI-0206965 (Sigma, SML1540-5MG).

Zhu Y., Fujimaki M., Snape L., Lopez A., Fleming A, & Rubinsztein D.C. (2024). Loss of WIPI4 in neurodegeneration causes autophagy-independent ferroptosis. Nature Cell Biology, 26(4), 542-551.

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Publication 2024

Top products related to «Necrostatin-1»

Necrostatin 1 by Merck Group

Sourced in United States, Germany, United Kingdom, China, Sao Tome and Principe, Morocco

Necrostatin-1 is a laboratory compound that functions as a selective inhibitor of necroptosis, a form of programmed cell death. It acts by blocking the receptor-interacting serine/threonine-protein kinase 1 (RIPK1), a key regulator of necroptosis. Necrostatin-1 is commonly used in scientific research to study cell death pathways.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Necrostatin 1 by Selleck Chemicals

Sourced in United States, China

Necrostatin-1 is a chemical compound that functions as a selective inhibitor of necroptosis, a form of programmed cell death. It acts by blocking the receptor-interacting protein kinase 1 (RIPK1), a key regulator of the necroptosis pathway. Necrostatin-1 has been widely used in research to study the mechanisms and implications of necroptosis in various biological systems.

Z vad fmk by Selleck Chemicals

Sourced in United States, China, Germany, Switzerland, United Kingdom

Z-VAD-FMK is a broad-spectrum caspase inhibitor. It functions by irreversibly binding to the catalytic site of caspase enzymes, thereby blocking their activity.

Dimethyl sulfoxide (dmso) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh

DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

Z vad fmk by Merck Group

Sourced in United States, Germany, China, Canada, Italy, Sao Tome and Principe, United Kingdom, Japan, Macao, Australia

Z-VAD-FMK is a caspase inhibitor that functions as a broad-spectrum inhibitor of caspase enzymes. It is commonly used in research applications to study apoptosis and cell death processes.

Ferrostatin 1 by Merck Group

Sourced in United States, Germany, United Kingdom, Italy, Japan

Ferrostatin-1 is a chemical compound used in research laboratories. It functions as a potent inhibitor of ferroptosis, a form of programmed cell death. Ferrostatin-1 is utilized in various experimental settings to study cellular mechanisms and pathways related to ferroptosis.

Necrostatin 1 by MedChemExpress

Sourced in United States, China

Necrostatin-1 is a small-molecule inhibitor of necroptosis, a form of programmed cell death. It acts by inhibiting the receptor-interacting serine/threonine-protein kinase 1 (RIPK1), a key mediator of necroptosis. Necrostatin-1 is commonly used in research applications to study the mechanisms and therapeutic potential of necroptosis.

Nec 1 by Merck Group

Sourced in United States, Germany, Sao Tome and Principe, United Kingdom

Nec-1 is a laboratory instrument designed for the analysis and quantification of cellular apoptosis. It is an established tool for researchers studying programmed cell death mechanisms and their implications in various biological processes and disease states.

Ferrostatin 1 by Selleck Chemicals

Sourced in United States, China

Ferrostatin-1 is a chemical compound used as a research tool in laboratory settings. It functions as a selective inhibitor of ferroptosis, a form of programmed cell death. The core purpose of Ferrostatin-1 is to serve as an experimental tool for investigating cellular processes and mechanisms related to ferroptosis.

What is Necrostatin-1 and how does it work?

Necrostatin-1 is a small-molecule inhibitor of necroptosis, a form of programmed cell death. It works by targeting the receptor-interacting protein kinase 1 (RIPK1), a key mediator of necroptosis. Necrostatin-1 has been shown to be effective in preclinical models in preventing cell death and reducing tissue damage in various diseases like neurodegeneration, ischemia-reperfusion injury, and inflammatory disorders.

What are some common applications of Necrostatin-1 in research?

Necrostatin-1 has been widely studied for its therapeutic potential in a variety of diseases. Some of the key applications include investigating its effects in models of neurodegeneration, ischemia-reperfusion injury, and inflammatory disorders. Researchers are particularly interested in Necrostatin-1's ability to prevent cell death and reduce tissue damage in these conditions.

Are there different variations or types of Necrostatin-1?

What are some common challenges in using Necrostatin-1 effectively?

One of the main challenges in using Necrostatin-1 effectively is ensuring reproducibility and accuracy of the protocols. Researchers may encounter issues with dosage, timing of administration, and ensuring sufficient target engagement. Additionally, there can be variability in the quality and potency of Necrostatin-1 products, which can impact the reliability of the results.

How can PubCompare.ai help researchers optimize their use of Necrostatin-1?

PubCompare.ai's AI-driven platform can be a valuable tool for researchers working with Necrostatin-1. The platform allows you to more efficiently screen protocol literature, leveraging AI to pinpoint critical insights. This can help researchers identify the most effective protocols related to Necrostatin-1 for their specific research goals. The platform's AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy. This can be particularly helpful in ensuring the optimal use and comparison of Necrostatin-1 in your research.

More about "Necrostatin-1"

Necrostatin-1 (Nec-1) is a powerful small-molecule inhibitor of necroptosis, a form of programmed cell death distinct from apoptosis.
This compound has garnered significant attention from researchers due to its potential therapeutic applications in a wide range of diseases, including neurodegenerative disorders, ischemia-reperfusion injury, and inflammatory conditions.
Necroptosis is a regulated cell death pathway that is mediated by the receptor-interacting protein kinase 1 (RIPK1), a key signaling molecule.
Necrostatin-1 exerts its effects by specifically targeting and inhibiting RIPK1, thereby preventing the initiation and progression of necroptosis.
This makes Nec-1 a valuable tool for investigating the role of necroptosis in various pathological scenarios and exploring its therapeutic potential.
In preclinical studies, Necrostatin-1 has demonstrated efficacy in reducing cell death and mitigating tissue damage in a variety of disease models.
Researchers have utilized Nec-1 in combination with other compounds, such as the pan-caspase inhibitor Z-VAD-FMK and the ferroptosis inhibitor Ferrostatin-1, to better understand the interplay between different cell death pathways and develop more comprehensive therapeutic strategies.
When working with Necrostatin-1, it is important to consider factors like solubility, stability, and potential interactions with other reagents, such as DMSO and fetal bovine serum (FBS).
PubCompare.ai's AI-driven platform can be a valuable resource for researchers, as it helps identify the most reproducible and accurate protocols for Nec-1 usage, as well as the best products and suppliers to optimize research outcomes.