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Nipagin

Nipagin, also known as methyl parahydroxybenzoate, is a commonly used preservative in a variety of pharmaceutical, cosmetic, and food products.
It helps prevent microbial growth and extend the shelf-life of these formulations.
PubCompare.ai's AI-powered platform allows researchers to easily locate, compare, and optimize protocols from literature, pre-prints, and patents related to the use of Nipagin, ensuring improved reproducibility and identification of the best products for their research needs.
Streamline your Nipagin research with PubCompare.ai's intellligent protocol comparisons.

Most cited protocols related to «Nipagin»

1
Drosophila Experimental Protocols
Cited 13 times
In all experiments except those for RU486 calibration, we used our laboratory stock of outbred wild type Drosophila melanogaster, Dahomey, which has been cured of Wolbachia by tetracycline treatment 1 . Flies were maintained in large population cages with overlapping generations at 25 °C with a 12 h: 12 h light: dark cycle. UAS-ArcAβ42 and elavGS transgenic flies were backcrossed into w1118, as reported in 2 and wDah; dilp2-31,5 deletion mutants and Wolbachia-positive white Dahomey (wDah) controls are those reported in 3 . All genetic constructs were back-crossed into the genetic background of their control for at least six generations before experiments were performed.
For all experiments using adult flies, other than food choice, flies were reared on sugar, yeast food (1SYBrewer’s; 1SY) as described in 4 for lifespan experiments. Egg collections were used to synchronize fly age as described in 5 . Flies for the food choice assay were reared in a medium containing, per liter, 80 g cane molasses, 22 g beetroot syrup, 8 g agar, 80 g corn flour, 10 g soya flour, 18 g yeast extract, 8 ml propionic acid, 12 ml nipagin (15% in ethanol).
Piper M.D., Blanc E., Leitão-Gonçalves R., Yang M., He X., Linford N.J., Hoddinott M.P., Hopfen C., Soultoukis G.A., Niemeyer C., Kerr F., Pletcher S.D., Ribeiro C, & Partridge L. (2013). A holidic medium for Drosophila melanogaster. Nature methods, 11(1), 10.1038/nmeth.2731.
Publication 2013
Adult Agar Animals, Transgenic Biological Assay Canes Carbohydrates Corn Flour Deletion Mutation Diptera Drosophila melanogaster Ethanol Food Gene Expression Regulation Light Molasses Nipagin propionic acid R-38486 Reproduction Saccharomyces cerevisiae Soybean Flour Tetracycline Wolbachia
2
Drosophila Experimental Protocols
Cited 11 times
In all experiments except those for RU486 calibration, we used our laboratory stock of outbred wild type Drosophila melanogaster, Dahomey, which has been cured of Wolbachia by tetracycline treatment 1 . Flies were maintained in large population cages with overlapping generations at 25 °C with a 12 h: 12 h light: dark cycle. UAS-ArcAβ42 and elavGS transgenic flies were backcrossed into w1118, as reported in 2 and wDah; dilp2-31,5 deletion mutants and Wolbachia-positive white Dahomey (wDah) controls are those reported in 3 . All genetic constructs were back-crossed into the genetic background of their control for at least six generations before experiments were performed.
For all experiments using adult flies, other than food choice, flies were reared on sugar, yeast food (1SYBrewer’s; 1SY) as described in 4 for lifespan experiments. Egg collections were used to synchronize fly age as described in 5 . Flies for the food choice assay were reared in a medium containing, per liter, 80 g cane molasses, 22 g beetroot syrup, 8 g agar, 80 g corn flour, 10 g soya flour, 18 g yeast extract, 8 ml propionic acid, 12 ml nipagin (15% in ethanol).
Piper M.D., Blanc E., Leitão-Gonçalves R., Yang M., He X., Linford N.J., Hoddinott M.P., Hopfen C., Soultoukis G.A., Niemeyer C., Kerr F., Pletcher S.D., Ribeiro C, & Partridge L. (2013). A holidic medium for Drosophila melanogaster. Nature methods, 11(1), 10.1038/nmeth.2731.
Publication 2013
Adult Agar Animals, Transgenic Biological Assay Canes Carbohydrates Corn Flour Deletion Mutation Diptera Drosophila melanogaster Ethanol Food Gene Expression Regulation Light Molasses Nipagin propionic acid R-38486 Reproduction Saccharomyces cerevisiae Soybean Flour Tetracycline Wolbachia
3
Drosophila Dietary Restriction Lifespan Protocol
Cited 11 times
Wild type Dahomey flies were housed and maintained as described in Bass et al. (2007) [7] (link). The chico1 allele is maintained as a balanced stock that has been backcrossed to the Dahomey outbred laboratory population as described in Clancy et al. (2001) [21] (link). snw, ry506, to1 (takeout) flies were a gift from Brigitte Dauwalder. All flies were maintained at 25°C, 65% humidity, on a 12h∶ 12h light∶ dark cycle. Unless stated otherwise, all assays used mated females at day 7 after eclosion. Day 7 was chosen because the flies are still young, but several early adult developmental processes have been completed [39] (link). All flies were reared for assays at a standard density, as for lifespan studies [40] (link), and allowed to mate for 48 h post emergence before being sorted by sex, under light CO2 anaesthesia, into 30 mL glass vials containing 7 mL food.
The DR food medium contained 100 g autolysed Brewer's yeast powder (MP Biomedicals, Ohio, USA), 50 g sugar, 15 g agar, 30 ml nipagin (100 g/L), and 3 mL propionic acid made up to 1 litre of distilled water. The full fed food contained 200 g autolysed yeast powder, 50 g sugar, 15 g agar, 30 ml nipagin (100 g/L), and 3 ml propionic acid made up to 1 litre of distilled water [7] (link). In the diet comparison experiment, this medium is labelled SYBrewer's. CSYExtract was made according to [15] (link). This was made by co-diluting sugar and yeast extract (Bacto Yeast extract, B.D. Diagnostics, Sparks, MD) in a binder of cornmeal (80 g/L), bacto-agar (0.5%) and propionic acid (10 g/L). The 1× concentration contained 10 g/L sucrose and 10 g/L yeast extract.
For DR lifespan experiments, flies were maintained 5 per vial at 25°C, 65% humidity, on a 12h∶ 12h light∶ dark cycle. Proboscis-extension assays were performed for 60 minutes at 5-minute intervals, 4 hours after lights-on at 21 separate days across the lifespan experiment.
Wong R., Piper M.D., Wertheim B, & Partridge L. (2009). Quantification of Food Intake in Drosophila. PLoS ONE, 4(6), e6063.
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Publication 2009
Adult Agar Alleles Anesthesia Bass Biological Assay Carbohydrates Diagnosis Diet Diptera Females Food Humidity Light Nipagin Powder propionic acid Saccharomyces cerevisiae Sucrose Yeast, Dried
4
Standardized Drosophila Lifespan Experiments
Cited 10 times
Experimental flies were raised at a standard density of 400–450 eggs per 200-ml bottle [52 ] on standard SY medium (1,000 ml distilled water, 100 g autolysed yeast powder, 100 g sucrose, 20 g agar, 30 ml Nipagin (100 gl–1), 3 ml propionic acid). Adults were collected over a 24-h period and transferred without anaesthesia to fresh SY food for 48 h and allowed to mate. Females were then collected using light CO2 anaesthesia and assigned randomly to the food regimes (Table S1). All experiments were done with mated females. Flies were kept on 35 ml of food at an initial density of 100 individuals per 200-ml bottle and transferred without anaesthesia to fresh food every 2–3 d. Deaths were scored 5–6 d a week and initial sample sizes (n0) were calculated as the summed death and censor observations over all ages. To minimise any density effects on mortality, two bottles within cohorts were merged when the density of flies reached 50 ± 10. To standardise the effects of parental age on offspring fitness [53 (link)], parents of experimental flies were of the same age and reared at a constant density.
Mair W., Piper M.D, & Partridge L. (2005). Calories Do Not Explain Extension of Life Span by Dietary Restriction in Drosophila. PLoS Biology, 3(7), e223.
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Publication 2005
Adult Agar Anesthesia Diptera Eggs Females Food Light Nipagin Parent Powder propionic acid Sucrose Yeast, Dried
5
Drosophila Husbandry and Genetics
Cited 8 times
Flies were raised on standard agar, cornmeal, molasses, and yeast food containing 1.5% Nipagin bought from IMBA (Vienna, Austria). Adults were placed in cages in a Percival DR 36VL incubator maintained at 29° and 65% humidity, and embryos were collected on standard plates prepared in house from apple juice, sugar, agar, and Nipagin, and treated with yeast from Lesaffre (Marcq, France). This applies to all experiments except the QF2 movie, whose fly husbandry conditions are described below. repo-GAL4 and QUAS-CD8::GFP were obtained from the Bloomington Drosophila Stock Center, UAS-moe::mCherry from P. Martin (Millard and Martin 2008 (link)), hml-dsRed from K. Brückner, and srp-GAL4 UAS-2xeGFP from R. Reuter.
Gyoergy A., Roblek M., Ratheesh A., Valoskova K., Belyaeva V., Wachner S., Matsubayashi Y., Sánchez-Sánchez B.J., Stramer B, & Siekhaus D.E. (2018). Tools Allowing Independent Visualization and Genetic Manipulation of Drosophila melanogaster Macrophages and Surrounding Tissues. G3: Genes|Genomes|Genetics, 8(3), 845-857.
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Publication 2018
Adult Agar Carbohydrates Drosophila Embryo Food Humidity Molasses Nipagin Yeasts

Most recents protocols related to «Nipagin»

1
Drosophila Models for Anti-Epileptic Treatments
The study was conducted on the male adult or post-eclosion D. melanogaster flies at 10 days of age at the time of study initiation following previous studies on bang-senseless (bss) D. melanogaster models (parabss) and novel anti-epileptic treatments.15 (link),16 (link) The Drosophila models of the bang sensitive (BS) family used in the study were the bang senseless or bss1 mutants of the paralytic gene (parabss1),48 ,49 (link) and the wild-type strains used as controls were Oregon-R or OrR and Canton-Special (CS).49 (link)-51 (link) Male flies were used as a previous laboratory study has shown differences between female and male bss Drosophila because the parabss is located on the X chromosome, and heterozygous parabss/+ female Drosophila have significantly decreased recovery times.52 (link) Only animals that underwent seizures were included in the study during behavioral (convulsion and learning/memory tests) and histological assessments.49 (link)The bang-sensitive mutant flies (parabss1) obtained from Prof. Richard Baines laboratory (University of Manchester, UK)48 ,49 (link) were used, and the wild-type flies ie, OrR (BDSC stock #25211) and CS (BDSC stock #64349), were obtained from Bloomington Drosophila Stock Center, Indiana University, U.S.A, and all the stocks were delivered to the Institute of Biomedical Research for culturing into sufficient stock colonies. The bss paralytic (para) D. melanogaster mutant flies used in the study have a gain-of-function mutation in the para sodium channel gene located at the para locus in neurons of the mutant flies.52 (link)-54 The flies were cultured on a standard yeast/cornmeal/agar diet (distilled water, 6.65% cornmeal, 7.15% dextrose, 5% yeast, 0.66% industrial agar supplemented with 3.4 mL/L propionic acid, and 2.2% nipagin) for Drosophila flies following previous methods,55 (link),56 (link) (Supplementary file 1). The flies were kept in incubators at 25°C, 65% humidity, and on a 12-h light/dark cycle (Fly incubator with programmable day/night cycle; Powers Scientific Inc., S33SD, USA). Flies were transferred to fresh plastic fly vials (Genesee Scientific, 32-116, USA), every 3 days, and fly density was kept to 10 flies per plastic fly vial (Genesee Scientific, 32-116, USA).
Ssempijja F., Dare S.S., Bukenya E.E., Kasozi K.I., Kenganzi R., Fernandez E.M, & Vicente-Crespo M. (2023). Attenuation of Seizures, Cognitive Deficits, and Brain Histopathology by Phytochemicals of Imperata cylindrica (L.) P. Beauv (Poaceae) in Acute and Chronic Mutant Drosophila melanogaster Epilepsy Models. Journal of Evidence-based Integrative Medicine, 28, 2515690X231160191.
Publication 2023
Adult Agar Animals Antiepileptic Agents Diet Drosophila Females Gain of Function Mutation Genes Glucose Heterozygote Humidity Males Memory Neurons Nipagin propionic acid Seizures Sodium Strains X Chromosome Yeast, Dried
2
Culturing Drosophila Fly Strain Canton S
The wild-type
strain of Drosophila fly (Canton S) was generously
gifted by Dr. Yasir Hasan Siddique, Department of Zoology, Faculty
of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh,
India. The flies were maintained and reared on a standard cornmeal
medium containing yeast granules as the protein source, agar–agar,
and nipagin (preservative) at constant temperature (23 ± 2 °C)
and 70–80% relative humidity under a 12 h dark/light cycle.
Diet was prepared according to a standard protocol. One liter of semisolid
diet contained 50 g of corn flour, 35 g of sucrose (carbohydrate source),
10 g of agar–agar (solidifying agent), 5 mL of propionic acid
(antifungal agent), and 15 g of yeast granules added to ensure availability
of the protein source. After 24 h, Drosophila flies
were transferred to stock bottles to avoid sticking flies to the media.
Rasheed M.Z., Khatoon R., Talat F., Alam M.M., Tabassum H, & Parvez S. (2023). Melatonin Mitigates Rotenone-Induced Oxidative Stress and Mitochondrial Dysfunction in the Drosophila melanogaster Model of Parkinson’s Disease-like Symptoms. ACS Omega, 8(8), 7279-7288.
Publication 2023
Agar Antifungal Agents Carbohydrates Corn Flour Cytoplasmic Granules Diet Diptera Drosophila Humidity Nipagin Pharmaceutical Preservatives propionic acid Proteins Sucrose Yeast, Dried
3
Antimicrobial Hydrogel Formulations with Propolis and Methylene Blue
Hydrogels were prepared by mechanical stirring of BNC pulp with 1% of the gelling agent Natrosol® (hydroxyethyl cellulose), 0.18% and 0.02% of the preservatives Nipagin® (methylparaben) and Nipazol® (propylparaben), respectively, and 5% of the humectant propylene glycol, all measured in w/w relative to the total mass (100%) of the hydrogel. Standard BNC hydrogel containing 1% dry mass of BNC was produced, named BNCH. From BNCH, formulations were prepared by adding 1.2, 2.4, and 3.6% (w/w) propolis extract (EPP-AF®) provided by Apis Flora Company (Ribeirão Preto, São Paulo, Brazil). The samples were named BNCH/P1, BNCH/P2, and BNCH/P3, respectively. Propolis concentrations were based on the study of Berretta et al. (2012) [24 (link)]. Natrosol® hydrogel was also produced as a control group and named BH.
For photodynamic inactivation, BNCH/P1 hydrogels were reproduced with the addition of MB at concentrations of 0.01 and 0.1% (w/w), originating samples BNCH/P1/MB1 and BNCH/P1/MB2, respectively. Formulations containing only BNC hydrogel and MB (BNCH/MB1 and BNCH/MB2) were also produced, keeping the same reagent concentrations.
Gonçalves I.S., Lima L.R., Berretta A.A., Amorim N.A., Pratavieira S., Corrêa T.Q., Nogueira F.A, & Barud H.S. (2023). Antimicrobial Formulation of a Bacterial Nanocellulose/Propolis-Containing Photosensitizer for Biomedical Applications. Polymers, 15(4), 987.
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Publication 2023
Apis Dental Pulp Humectants Hydrogels hydroxyethylcellulose methylparaben Nipagin Nipazol PEGDMA Hydrogel Pharmaceutical Preservatives Propolis Propylene Glycol propylparaben
4
Standardized Yeast-Sugar Food Protocol
Our sugar-yeast (SY) food contained sugar (Bundaberg, M180919) (50 g/L), brewer’s yeast (MP Biomedicals, 903312) (100 g/L), agar (Gelita, A-181017) (10 g/L), propionic acid (Merck, 8.00605.0500) (3 mL/L) and nipagin (Sigma-Aldrich, W271004-5KG-K) (12 g/L), prepared as in [16 (link)]. Refer to Table 1 for more detailed product information.
Gómez Ortega J., Raubenheimer D., Tyagi S., Mirth C.K, & Piper M.D. (2023). Biosynthetic constraints on amino acid synthesis at the base of the food chain may determine their use in higher-order consumer genomes. PLOS Genetics, 19(2), e1010635.
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Publication 2023
Agar Carbohydrates Food Nipagin propionic acid Saccharomyces cerevisiae Yeast, Dried
5
Drosophila Harwich Strain Maintenance

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Agar Diet Drosophila Drosophila melanogaster Functional Food Nipagin Pharmaceutical Preservatives Saccharomyces cerevisiae Strains

Top products related to «Nipagin»

1
Nipagin by Merck Group
Sourced in United States, Germany, Spain, United Kingdom
Nipagin is a preservative used in the manufacturing of various products, including pharmaceuticals, cosmetics, and personal care items. It serves as an antimicrobial agent, helping to prevent the growth of microorganisms in these formulations.
2
Propionic acid by Merck Group
Sourced in United States, Germany, China, United Kingdom, Croatia, Sao Tome and Principe, Macao, Italy, Japan, Singapore, Australia
Propionic acid is a widely used organic compound that serves as a key ingredient in various industrial and laboratory applications. It is a colorless, pungent liquid with a characteristic odor. Propionic acid is primarily utilized as a preservative and antimicrobial agent in food, animal feed, and pharmaceutical products.
3
Agar by Merck Group
Sourced in United States, Germany, United Kingdom, Spain, India, France, China, Italy, Ireland, Romania, Australia, Belgium, Hungary, Austria, Greece, Switzerland
Agar is a gelatinous substance derived from red algae that is commonly used as a growth medium in microbiology. It provides a solid, nutrient-rich substrate for the cultivation of various microorganisms, including bacteria, fungi, and some algae. Agar is known for its thermal stability, resistance to microbial degradation, and ability to support the growth of a wide range of microbes.
4
Propionic acid by Thermo Fisher Scientific
Sourced in Belgium, Germany, China, United States
Propionic acid is a colorless, liquid carboxylic acid with a pungent odor. It is commonly used in the chemical industry as a preservative, antimicrobial agent, and as an intermediate in the production of various organic compounds.
5
W1118 by Bloomington Drosophila Stock Center
Sourced in United States
W1118 is a wild-type Drosophila melanogaster strain commonly used as a genetic background. It serves as a standard reference strain for various experimental studies in the field of Drosophila research.
6
Ru486 by Merck Group
Sourced in United States, Germany, United Kingdom, Israel, Canada
RU486 is a laboratory product manufactured by Merck Group. It is a synthetic steroid compound used for research purposes. The core function of RU486 is to act as an antiprogesterone agent.
7
Propionic acid by Carl Roth
Sourced in France
Propionic acid is a colorless liquid organic compound with a pungent odor. It is a naturally occurring short-chain fatty acid.
8
Sucrose by Merck Group
Sourced in United States, Germany, United Kingdom, France, Switzerland, Sao Tome and Principe, China, Macao, Italy, Poland, Canada, Spain, India, Australia, Belgium, Japan, Sweden, Israel, Denmark, Austria, Singapore, Ireland, Mexico, Greece, Brazil
Sucrose is a disaccharide composed of glucose and fructose. It is commonly used as a laboratory reagent for various applications, serving as a standard reference substance and control material in analytical procedures.
9
Sodium alginate by Fagron
Sourced in Spain
Sodium alginate is a naturally derived polysaccharide that is commonly used as a thickening, stabilizing, and emulsifying agent in various pharmaceutical and personal care products. It is extracted from brown seaweed and has the ability to form viscous solutions when dissolved in water. Sodium alginate is a versatile excipient that can be utilized in a wide range of formulations, including topical gels, creams, and lotions.
10
Glucose by Merck Group
Sourced in United States, Germany, United Kingdom, China, France, Italy, Spain, Sao Tome and Principe, Macao, Switzerland, Poland, Japan, India, Canada, Belgium, Denmark, Australia, Sweden, Brazil, Austria, Singapore, Israel, Portugal, Argentina, Mexico, Norway, Greece, Ireland
Glucose is a laboratory equipment used to measure the concentration of glucose in a sample. It is a fundamental tool in various medical and scientific applications, including the diagnosis and monitoring of diabetes, metabolic research, and food analysis.

More about "Nipagin"

Nipagin, also known as methyl parahydroxybenzoate, is a widely used preservative in a variety of pharmaceutical, cosmetic, and food products.
It helps prevent microbial growth and extend the shelf-life of these formulations.
Nipagin is related to other preservatives like propionic acid, which is used to inhibit mold growth.
It may be used in combination with other ingredients like agar, a gelling agent, or sucrose and sodium alginate, which can modify texture and viscosity.
PubCompare.ai's innovative AI-powered platform enables researchers to easily locate, compare, and optimize protocols involving Nipagin from literature, pre-prints, and patents.
This ensures improved reproducibility and identification of the best products for their research needs, whether it's studying the effects of Nipagin on microbial cultures like W1118 yeast or investigating the interactions between Nipagin and compounds like the drug RU486.
Streamline your Nipagin research with PubCompare.ai's intellligent protocol comparisons and uncover the optimal conditions and techniques for your specific project.
Leverage the power of AI to take your Nipagin-related studies to the next level.