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Nobiletin
Nobiletin
Nobiletin is a natural flavonoid compound found in citrus fruits, particularly in the peel of mandarin oranges.
This bioactive compound has been extensively studied for its potential therapeutic benefits, including anti-inflammatory, antioxidant, and neuroprotective properties.
Nobiletin has shown promise in the treatment of various conditions, such as metabolic disorders, neurodegenerative diseases, and cancer.
Reserchers can leverage PubCompare.ai's AI-driven comparisons to optimize their nobiletin research, locate relevant data from literature, preprints, and patents, and achieve reproducible, accurate findings.
Experince the power of PubCompare's intelligent tools today to enhance your nobiletin-related studies.
This bioactive compound has been extensively studied for its potential therapeutic benefits, including anti-inflammatory, antioxidant, and neuroprotective properties.
Nobiletin has shown promise in the treatment of various conditions, such as metabolic disorders, neurodegenerative diseases, and cancer.
Reserchers can leverage PubCompare.ai's AI-driven comparisons to optimize their nobiletin research, locate relevant data from literature, preprints, and patents, and achieve reproducible, accurate findings.
Experince the power of PubCompare's intelligent tools today to enhance your nobiletin-related studies.
Most cited protocols related to «Nobiletin»
Biological Assay
Body Weight
Diet
Energy Metabolism
Males
Mice, House
Obesity
Sulfoxide, Dimethyl
Treatment Protocols
Tube Feeding
Data collection and processingCytotoxicity and mRNA arrays data were obtained from the NCI 60 DTP website (http.dtp.nci.nih.gov) (Monks et al., 1997). COMPARE analysis with the public library produces a list of drugs that have similarities with nobiletin, as well as a list of gene expressions on the NCI 60 cell line panel (Mahmoud et al., 2018). The similarity pattern is expressed as the Pearson correlation coefficient. In this study, the list of compounds and genes was limited to the Pearson correlation coefficient <-0.5 and> 0.5.
Functional and pathway enrichment analysisGene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were carried out by The Database for Annotation, Visualization and Integrated Discovery (DAVID) v6.8 (Huang da et al., 2009), with p<0.05 was selected as the cutoff value. Moreover, pathway enrichment was also conducted busing Overrepresentation Enrichment Analysis (ORA) from WEB-based GEne SeT AnaLysis Toolkit (WebGestalt) with FDR<0.05 was selected as the cutoff value (Wang et al., 2017a).
Construction of PPI network and module analysisProtein-protein interaction (PPI) network was constructed with STRING-DB v11.0 (Szklarczyk et al., 2015). Confidence scores >0.7 were considered significant. PPI network was visualized by Cytoscape software. Genes with a degree score more than 5, analyzed by CytoHubba plugin, were selected as hub genes.
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Cell Lines
DNA Library
Gene Expression
Genes
Genome
Monks
nobiletin
Pharmaceutical Preparations
Proteins
RNA, Messenger
Total RNA was extracted from frozen calf muscle by applying TRizol method (Invitrogen). Two micrograms of extracted RNA were used for cDNA synthesis. Gene expression was analyzed by using Mx3000p (Agilent technologies). Primer sequences are listed in the Supplementary Information (Supplementary Table 2 ).
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Anabolism
DNA, Complementary
Freezing
Gene Expression
Muscle Tissue
Oligonucleotide Primers
trizol
Sulphorhodamine B (SRB) assays were used for cell density determination, based on sensitive measure of total cellular protein, which perform similarly compared with other proliferation assays such as MTT assay44 (link). Briefly, cells were seeded into flat bottomed 96-well plates at an initial density of 7.5 × 103 per well before treatment. Cells were exposed to varying concentrations of nobiletin (9, 4.5, 1.5 and 0.5 μM) and combined them with varying concentrations of PTX (1 μM to 0.03 nM with 3.16 fold diluted, 10 μM to 0.3 nM with 3.16 fold diluted, 100 μM to 3 nM with 3.16 fold diluted respectively) to test whether this combination can enhance the growth inhibition of MDR cancer cells. After removing the medium, cells were fixed in 10% trichloroacetic acid for 1 h at 4 °C and then washed with water five times. 0.4% SRB dissolved in 1% v/v acetic acid was added and incubated 30 min for staining. The cells were quickly washed with 1% acetic acid and left to dry overnight. The protein bound SRB was solubilized by adding 200 μl 10 mM Tris buffer per well and was measured at wavelengths 490 nm using a plate reader (Spectra MAX 250; Molecular Devices, Sunnyvale, CA). The degree of resistance was estimated by comparing the IC50 (concentration of 50% inhibition) for the MDR cells to that of parent sensitive cells, while, the degree of reversal of MDR was calculated by dividing the IC50 for cells with the chemotherapeutic drugs in the absence of nobiletin by that obtained in the presence of nobiletin.
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Acetic Acid
Biological Assay
lissamine rhodamine B
Malignant Neoplasms
Medical Devices
nobiletin
Parent
Pharmaceutical Preparations
Pharmacotherapy
Proteins
Psychological Inhibition
Trichloroacetic Acid
Tromethamine
The tumor invasion assay was performed using a modified Boyden chamber (Neuro Probe, Cabin John, MD) as described elsewhere [65 (link)]. Matrigel (Collaborative Biomedical Products, Bedford, MA) was diluted with cold filtered distilled water to receive the concentration 25 μg/50 μl and applied to the upper to 8 μm pore size polycarbonate membrane filters of the upper well. Briefly, U2OS and HOS cells were treated with indicated concentrations (0, 25, 50, 75, 100 μM) of nobiletin for 24 h. The migrated cells passed through the basement membrane layer and clung to the bottom of the Boyden chamber membrane, while non-invasive cells stayed in the upper chamber. The data were presented as the average number of cells attached to the bottom surface from randomly chosen files under a light microscope. In order to measure the ability of osteosarcoma cells on migration, cells were seeded into the upper well of Boyden chamber which were not coated with Matrigel. Migration of cells treated with different concentrations of nobiletin was measured as described in the cell invasion assay.
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Biological Assay
Cells
Common Cold
Light Microscopy
matrigel
Membrane, Basement
Migration, Cell
Neoplasm Invasiveness
nobiletin
Osteosarcoma
polycarbonate
Strains
Tissue, Membrane
Most recents protocols related to «Nobiletin»
Male C57BL/6J mice (7 weeks old) were purchased from Charles River Japan (Kanagawa, Japan) and used for the serum hormone assay and RT-PCR. Male ICR mice were purchased from Tokyo Laboratory Animals Science Co., Ltd. (Tokyo, Japan), and female PERIOD2::LUCIFERASE (PER2::LUC) knock-in mice (ICR background) bred at Waseda University were used for blood glucose measurements and in vivo imaging assays, respectively. The mice were all housed under conditions of controlled temperature (23 °C ± 3 °C), humidity (50% ± 20%), and lighting (lights on from 07:00 to 19:00 at Taisho Pharmaceutical Co., Ltd. or from 08:00 to 20:00 at Waseda University). In the 24 h cycle, Zeitgeber time 0 (ZT0) was designated as the time when the lights were turned on, and ZT12 was designated as the time when the lights were turned off. All the mice were given access to food and tap water ad libitum, and nobiletin (10–100 mg/kg; purchased from FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was administered by intraperitoneal (i.p.) injection in the vehicle (10 mL/kg body weight) containing 0.5% carboxymethyl cellulose (CMC). Rolipram (PDE4 inhibitor, FUJIFILM Wako Pure Chemical Corporation) and SR1078 (RORα/γ agonist, FUJIFILM Wako Pure Chemical Corporation) were also used for the experiment at 10 mg/kg in 0.5% CMC [12 (link)]. Mice were euthanized by rapid decapitation for whole blood samples.
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The RNs and UPNs were dissolved in deionized water at concentrations of 15.63, 31.25, 62.50, 125, 250, and 500 μg/mL, and a 0.22 μm filter was used after thorough ultrasonication. Six milligrams of porcine pancreatic lipase (PPL) was dissolved in 10 mL of buffered phosphate (0.1 M, pH 7.2). Then, as the reaction substrate, 8.4 μL of p-nitrophenylbutyrate (PNPB) was dissolved in 10 mL of acetonitrile. Different amounts of nobiletin sample solution (0.2 mL) were carefully mixed with 0.1 mL of PPL solution, and the mixture was then diluted to 2 mL with phosphate buffer. The samples were mixed evenly by ultrasonication and incubated at 37 °C for 30 min. An ultraviolet spectrophotometer was used to measure the absorbance at 410 nm, with orlistat serving as the positive control.
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The α-glucosidase inhibition experiment was performed according to the standard protocol, with some modifications [19] (link). RN and UPN were dispersed in deionized water and diluted to different concentrations (15.63, 31.25, 62.50, 125, 250, and 500 μg/mL). After complete ultrasonography, a 0.22 μm filter membrane was filtered for use. In 1 mL of phosphate buffer solution (0.1 M, pH 7.2), 2 μL of α-glucosidase was dissolved. The reaction substrate, 8 mmol/L 4-nitrophenyl-D-glucopyranoside (PNPG) solution, was made by adding 200 mg of powdered PNPG to 125 ml of phosphate buffer. First, 10.6 g of sodium carbonate was dissolved in 100 mL of deionized water. as a stopper. Different concentrations of the nobiletin sample solution (0.1 mL) and the α-glucosidase solution (0.1 mL) were mixed well (2 μL/mL), and each reaction mixture received 200 μL of PNPG solution. The reaction was terminated with Na2CO3. The sample absorbance was measured at 405 nm. The procedure's positive control was acarbose, which was used at the same concentration as the sample.
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After the generated powder sample was fully homogenized using ultrasonic treatment for 5 min, it was dispersed in deionized water. A 0.22 μm organic filter membrane was used for filtration, and an AB SCIEX API4000 type LC-MS/MS System was used for detection. The conditions of the experiment were as follows: The mobile phase was a solution of 0.1 % formic acid. The gradient elution method was used to inject 1 μL of methanol. The column temperature was 35 °C, and the flow rate was adjusted to 0.4 mL/min.
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Cisplatin (P4394, Sigma-Aldrich, USA) was dissolved in saline, nobiletin (HY-N0155, MCE, USA), ferrostatin-1 (HY-100579, MCE, USA), and Z-VAD-FMK (HY-16658B, MCE, USA) were dissolved in DMSO. These solutions were diluted in culture medium. After incubation overnight, the cells and cochlear explants were treated with or without 20 µM nobiletin for 8 h, followed by exposure to 20 µM Cisplatin for 24 h. Alternatively, cells and cochlear explants were treated with 20 µM nobiletin alone for 24 h. Furthermore, based on previous study9 (link), 30 µM ferrostatin-1 and 40 µM Z-VAD-FMK were employed as inhibitors of ferroptosis and apoptosis, respectively, to pretreat cells for 2 h, followed by exposure to 20 µM Cisplatin for 24 h.
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Top products related to «Nobiletin»
Sourced in United States, Germany
Nobiletin is a lab equipment product manufactured by Merck Group. It is a naturally occurring flavonoid compound that can be isolated and used for research purposes. The core function of Nobiletin is to serve as a chemical standard and reference material for analytical and scientific applications.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
Sourced in China
Nobiletin is a bioactive compound extracted from citrus fruits, such as mandarin oranges. It is a natural product that has been the subject of scientific research. The core function of Nobiletin is to serve as a valuable tool for researchers and scientists studying its potential biological and pharmacological properties.
Sourced in United States, Germany, France, United Kingdom, Italy, Morocco, Spain, Japan, Brazil, Australia, China, Belgium, Ireland, Denmark, Sweden, Canada, Hungary, Greece, India, Portugal, Switzerland
The Milli-Q system is a water purification system designed to produce high-quality ultrapure water. It utilizes a multi-stage filtration process to remove impurities, ions, and organic matter from the input water, resulting in water that meets the strict standards required for various laboratory applications.
Sourced in China
Naringenin is a flavanone compound commonly found in citrus fruits. It serves as a standard reference material for analytical procedures involving flavonoid identification and quantification.
Sourced in United States, Germany
Tangeretin is a laboratory equipment product manufactured by Merck Group. It is a compound used in various research and analytical applications. The core function of Tangeretin is to serve as a reference standard or analytical tool, but a detailed description of its intended use cannot be provided in a concise, unbiased, and factual manner.
Sourced in China
Narirutin is a laboratory equipment product. It is a natural flavonoid compound that can be used for analytical and research purposes.
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
More about "Nobiletin"
Nobiletin, a natural citrus flavonoid, has garnered significant attention for its diverse therapeutic potentials.
This bioactive compound, predominantly found in the peel of mandarin oranges (Citrus reticulata), has been extensively studied for its anti-inflammatory, antioxidant, and neuroprotective properties.
Researchers can leverage the power of PubCompare.ai's AI-driven comparisons to optimize their nobiletin-related studies, locate relevant data from literature, preprints, and patents, and achieve reproducible, accurate findings.
Nobiletin's pharmacological activities extend beyond its antioxidant and anti-inflammatory effects.
It has demonstrated promise in the management of metabolic disorders, neurodegenerative conditions, and even cancer.
Synonyms and related terms include tangeretin, naringenin, and narirutin, all of which are similarly found in citrus fruits and possess various biological activities.
To conduct robust nobiletin research, researchers may employ techniques such as cell culture experiments using fetal bovine serum (FBS) and dimethyl sulfoxide (DMSO) as solvents, as well as advanced analytical methods like high-performance liquid chromatography (HPLC) and mass spectrometry.
The use of a Milli-Q water purification system can ensure the high-quality water necessary for these experiments.
By leveraging PubCompare.ai's intelligent tools, researchers can streamline their nobiletin-related studies, optimize research protocols, and access a wealth of relevant data from diverse sources, including scientific literature, preprints, and patents.
This empowers researchers to make informed decisions, enhance the reproducibility of their findings, and ultimately contribute to the growing understanding of nobiletin's therapeutic potential.
This bioactive compound, predominantly found in the peel of mandarin oranges (Citrus reticulata), has been extensively studied for its anti-inflammatory, antioxidant, and neuroprotective properties.
Researchers can leverage the power of PubCompare.ai's AI-driven comparisons to optimize their nobiletin-related studies, locate relevant data from literature, preprints, and patents, and achieve reproducible, accurate findings.
Nobiletin's pharmacological activities extend beyond its antioxidant and anti-inflammatory effects.
It has demonstrated promise in the management of metabolic disorders, neurodegenerative conditions, and even cancer.
Synonyms and related terms include tangeretin, naringenin, and narirutin, all of which are similarly found in citrus fruits and possess various biological activities.
To conduct robust nobiletin research, researchers may employ techniques such as cell culture experiments using fetal bovine serum (FBS) and dimethyl sulfoxide (DMSO) as solvents, as well as advanced analytical methods like high-performance liquid chromatography (HPLC) and mass spectrometry.
The use of a Milli-Q water purification system can ensure the high-quality water necessary for these experiments.
By leveraging PubCompare.ai's intelligent tools, researchers can streamline their nobiletin-related studies, optimize research protocols, and access a wealth of relevant data from diverse sources, including scientific literature, preprints, and patents.
This empowers researchers to make informed decisions, enhance the reproducibility of their findings, and ultimately contribute to the growing understanding of nobiletin's therapeutic potential.