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NS 398

NS 398 is a selective cyclooxygenase-2 (COX-2) inhibitor commonly used in biomedical research.
It has been studied for its anti-inflammatory, analgesic, and potential antitumor properties.
PubCompare.ai's AI-driven platform can help researchers optimize their NS 398 protocols by easily locating relevant literature, preprints, and patents, and leveraging AI-powered comparisons to identify the best protocols and products for their needs.
This can enhance reproducibility and accuracy in NS 398-related research.

Most cited protocols related to «NS 398»

1
Zebrafish Embryo Chemical Screen
Cited 7 times
Zebrafish wild-type (WT) embryos were arrayed and treated with small molecules using the ICCB Known Bioactives Library as described (Poureetezadi et al., 2014 (link)). Zebrafish embryos were staged at 2 hpf, then at least 30 fertilized embryos were arrayed into the chambers of 24-well plates and incubated at 28°С in E3 media until just prior to 4 hpf. Working stocks of small molecules were stored at −80°С, then dissolved in 100% DMSO to make 10 mM concentrations (Lengerke et al., 2011 (link)). For drug exposure, the E3 media was completely drawn off the embryos using a glass transfer pipet and the appropriate solution of DMSO, PGB2, dmPGE2, indomethacin, SC-560, NS-398, AH6809, or PF04418948 was applied at a discrete development time point (eg 4 hpf or 12 ss) (American Bioanalytical, Enzo Life Sciences, Santa Cruz) (North et al., 2007 (link); Eisinger et al., 2007 (link); Jin et al., 2014 (link)). Embryos were raised to the 20 ss or 24 hpf, washed three times with E3, then fixed in 4% paraformaldehyde. For rescue of prostaglandin activity, ptgs1 or ptgs2a deficient embryos were generated, and cohorts of approximately 30 embryos were arrayed in 24-well plates with E3, then placed in a 28°С incubator until 4 hpf. E3 was then completely drawn off the wells using a glass transfer pipet and a 50 µM concentration of dmPGE2 was applied. The embryos were placed into a 28°С incubator, raised until 24 hpf, washed three times with E3, and fixed as previously described.
Poureetezadi S.J., Cheng C.N., Chambers J.M., Drummond B.E, & Wingert R.A. (2016). Prostaglandin signaling regulates nephron segment patterning of renal progenitors during zebrafish kidney development. eLife, 5, e17551.
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Publication 2016
16,16-Dimethylprostaglandin E2 AH 6809 cDNA Library Embryo Indomethacin NS 398 paraform Pharmaceutical Preparations prostaglandin B2 Prostaglandins PTGS1 protein, human SC 560 Sulfoxide, Dimethyl Zebrafish
2
Screening Zebrafish Embryos for Bioactive Compounds
Cited 5 times
Tübingen strain fish were used for the studies and staged as described (64 (link)). For screening, WT zebrafish embryos were arrayed in 96-well plates at 2 h post fertilization. At 4 hpf the embryo media were replaced with 1:100 dilutions of drugs from the ICCB Known Bioactives Library (Enzo Life Sciences) (18 ). For treatments with 50 μM SC-560, 50 μM NS-398, 30 μM indomethacin, or 100 μM dmPGE2 (American Bioanalytical, Enzo Life Sciences, Santa Cruz), embryos were arrayed in 12-well plates and treated protected from light at 60% epiboly. Drugs were removed at the 28 ss and embryos were fixed in 4% paraformaldehyde.
Marra A.N., Adeeb B.D., Chambers B.E., Drummond B.E., Ulrich M., Addiego A., Springer M., Poureetezadi S.J., Chambers J.M., Ronshaugen M, & Wingert R.A. (2019). Prostaglandin signaling regulates renal multiciliated cell specification and maturation. Proceedings of the National Academy of Sciences of the United States of America, 116(17), 8409-8418.
Publication 2019
16,16-Dimethylprostaglandin E2 DNA Library Embryo Fertilization Fishes Indomethacin Light NS 398 paraform Pharmaceutical Preparations SC 560 Strains Technique, Dilution Zebrafish
3
Cell Cycle Analysis by Flow Cytometry
Cited 5 times
TIVE or TIVE-LTC cells were either untreated or treated with drugs (Indo or NS-398) or solvent control as described for cell number and viability assays. Harvested cells were diluted to contain ∼106 cells/ml and DNA distribution analysis was performed. Cells were fixed with 70% ethanol overnight and DNA was stained with propidium iodide at a final concentration of 50 µg/ml with RNaseA (100 U/ml) prior to flow cytometry analysis using a LSRII (BD Biosciences). Data were analyzed using ModFit Lt V3 software (Verity Software House).
Sharma-Walia N., Paul A.G., Bottero V., Sadagopan S., Veettil M.V., Kerur N, & Chandran B. (2010). Kaposi's Sarcoma Associated Herpes Virus (KSHV) Induced COX-2: A Key Factor in Latency, Inflammation, Angiogenesis, Cell Survival and Invasion. PLoS Pathogens, 6(2), e1000777.
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Publication 2010
Biological Assay Cells Ethanol Flow Cytometry NS 398 Pharmaceutical Preparations Propidium Iodide Solvents
4
Isolation and Characterization of Human Bone Marrow MSCs
Cited 4 times
Bone marrow MSCs were derived from whole bone marrow from independent human donors (commercially available by AllCells) (Supporting Information Figure S1A). Briefly, mononuclear cells were enriched in the buffy layer of whole bone marrow by phase separation in Ficoll-Paque. Cells were either cryopreserved or resuspended for immediate expansion in complete culture medium consisting of MEM-α (Thermo Scientific), 20% fetal bovine serum (Atlanta Biologicals), 100 units/ml penicillin (Gibco), 100 μg/ml streptomycin (Gibco), and 2 mM L-glutamine (Gibco). Nonadherent cells were removed after 2 days. Adherent colonies were expanded further and frozen as Passage 1. MSCs were profiled on a BD LSR II flow cytometer for expression of surface markers consistent with minimal guidelines established by the International Society for Stem Cell Therapy, including CD90-BV421 (clone 5E10, BD Biosciences), CD73-PE (clone AD2, BD Biosciences), CD105-FITC (clone 266, BD Biosciences), CD45-APC-Cy7 (clone HI30, Biolegend), CD34-APC (clone 581, BD Biosciences), HLA-DR-PerCP-Cy5.5 (clone G46-6, BD Biosciences), and CD11b-PE-Cy7 (clone ICRF44, Biolegend), using Ghost Dye BV510 (Tonbo) for exclusion of dead cells (Supporting Information Figure S1B, C) [24 (link)]. Thawed MSCs were plated at 1×105 cells/ml, and medium was changed every three days. At 80% confluence, cells were passaged into IBIDI channels (μ-Slide VI 0.4) or large-scale fluidics at a density of 0.5–1.5×106 cells/ml for qRT PCR, immunoblotting, PGE2 ELISA, and rat CCI experiments and at 2×105 cells/ml for TNF-α ELISA experiments.
Cells were cultured with pharmacological compounds to block PGE2 production and/or signaling by incubation with 10 μM indomethacin or 10 μM NS-398 (Cayman Chemical). The stabilized synthetic analog dmPGE2 (Cayman Chemical) was applied at 10 μM. BAY 11-7085 was applied at a concentration of 50 ng/ml (Cayman Chemical).
Diaz M.F., Vaidya A.B., Evans S.M., Lee H.J., Aertker B.M., Alexander A.J., Price K.M., Ozuna J.A., Liao G.P., Aroom K.R., Xue H., Gu L., Omichi R., Bedi S., Olson S.D., Cox CS J.r, & Wenzel P.L. (2017). Biomechanical forces promote immune regulatory function of bone marrow mesenchymal stromal cells. Stem cells (Dayton, Ohio), 35(5), 1259-1272.
Publication 2017
16,16-Dimethylprostaglandin E2 BAY 11-7085 Biological Factors Bone Marrow Caimans Cardiac Arrest Cells Cell Therapy Clone Cells CY5.5 cyanine dye Dinoprostone Donors Enzyme-Linked Immunosorbent Assay Fetal Bovine Serum Ficoll Fluorescein-5-isothiocyanate Freezing Glutamine HLA-DR Antigens Homo sapiens Indomethacin ITGAM protein, human NS 398 NT5E protein, human Penicillins Red Cell Ghost Stem, Plant Stem Cells Streptomycin Therapeutics Thy-1 Antigens Tumor Necrosis Factor-alpha
5
Lung Fibroblast Behavior on Gradient Stiffness Gels
Cited 4 times
Lung fibroblasts (CCL-151) were cultured in Kaighn’s Modification of Ham’s F12 medium (F12K) supplemented with 10% FBS (Lonza), 100 U/ml penicillin, and 100 µg/ml streptomycin. Gradient stiffness gels were seeded at 50 cells/mm2 in 6-well plates. After 4 h, gels were gently washed with fresh medium and then moved into new wells. Cells were cultured for 5 d before fixation for immunostaining. For TGF-β1 treatment, 2 ng/ml TGF-β1 was added at day 2 and day 4. To generate panorama images, the entire gel width was imaged along the stiffness gradient by tiling 5–7 adjacent pictures. Cell density was determined by counting nuclei numbers on fluorescent images with MetaMorph 6.1. Density values were normalized to the global mean value from 4-h attachment. NHLFs were seeded at a density of 50 cell/mm2 in 96-well plates containing gels with five individual discrete stiffnesses: 0.1, 0.4, 1.6, 6.4, and 25.6 kPa. Cells were cultured with F12K containing 1% FBS at 37°C and 5% CO2. After 48 h, cell density was determined using CyQuant NF Cell Proliferation Assay (Invitrogen). Density values were normalized to the global mean value from 4-h attachment. For drug treatments, PGE2, butaprost, or NS-398 was added after 4-h initial attachment. In general, NHLFs or CCL-151 cells were cultured in 0.1% FBS for microarray and qPCR analysis to minimize differences in transcript levels caused by variations in proliferation and cell cycle progression. NHLFs were cultured in 1% FBS for proliferation, procollagen expression, PGE2, and collagen secretion analysis; CCL-151 cells grew more slowly and were cultured in 10% FBS for proliferation and procollagen expression analyses.
Liu F., Mih J.D., Shea B.S., Kho A.T., Sharif A.S., Tager A.M, & Tschumperlin D.J. (2010). Feedback amplification of fibrosis through matrix stiffening and COX-2 suppression. The Journal of Cell Biology, 190(4), 693-706.
Publication 2010
Biological Assay butaprost Cell Cycle Cell Nucleus Cell Proliferation Cells Collagen Dinoprostone Disease Progression Fibroblasts Gels Lung Microarray Analysis NS 398 Penicillins Pharmaceutical Preparations Procollagen secretion Streptomycin TGF-beta1

Most recents protocols related to «NS 398»

1
Bile Salts and Isoketals Induce p53 Modification

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Publication 2023
Acetylcysteine Acids Aftercare Buffers Caimans Cells Coculture Techniques Culture Media Cyclooxygenase 2 Inhibitors Eagle Epithelial Cells Gas Scavengers Homo sapiens Immunoglobulins isolevuglandin Keratinocyte Milk, Cow's NS 398 Pharmaceutical Preparations Pylorus Salts Salts, Bile Sodium Stomach Strains tempol Tissue, Membrane TP53 protein, human Western Blot
2
Zebrafish Nephron Regeneration Modulation
During the nephron regeneration stage, the Indo (I7378-5G, Sigma; 400 μM, 10 μL per fish), NS-398 (N194-5MG, Sigma; 140 μM, 10 μL per fish), TG4-155 (S6793, Selleck; 400 μM, 10 μL per fish), dmPGE2 (D0160, Sigma; 600 μM, 10 μL per fish), GW627368X (T1978, TOPSCIENCE; 200 μM, 10 μL per fish), XAV939 (S1180, Selleck; 200 μM, 10 μL per fish), ICRT 14 (HY-16665, MCE; 10 μM, 10 μL per fish), ICG-001 (HY-14428, MCE; 100 μM, 10 μL per fish), PKI (HY-P1290A, MCE; 20 μM, 10 μL per fish), and H89 (HY-15979, MCE; 200 μM, 10 μL per fish) were intraperitoneally injected into zebrafish at 2, 4, and 6 dpi. Kidneys were collected at 7 dpi for RNA extraction, WISH, or immunofluorescence. For the 5 dpi test, inhibitors were injected at 2 and 4 dpi, and samples were collected at 5 dpi; 1% DMSO was injected in control groups with the same conditions. For juvenile zebrafish experiments, dmPGE2 (2 μM in egg water) was used to test the influence on mesonephros development. 0.2% DMSO was used in control groups under the same conditions.
Liu X., Yu T., Tan X., Jin D., Yang W., Zhang J., Dai L., He Z., Li D., Zhang Y., Liao S., Zhao J., Zhong T.P, & Liu C. (2023). Renal interstitial cells promote nephron regeneration by secreting prostaglandin E2. eLife, 12, e81438.
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Publication 2023
16,16-Dimethylprostaglandin E2 Fishes Fluorescent Antibody Technique GW627368X ICG 001 inhibitors Kidney Mesonephros Nephrons NS 398 Regeneration Sulfoxide, Dimethyl TG4-155 XAV939 Zebrafish
3
Spectrophotometric and Chromatographic Analysis
UV–Vis double beam spectrophotometer Halo DB-20S (Dynamica GmbH, Dietikon, Switzerland) was used for all spectrophotometric measurements. The chemical and reagents used for the evaluation of phenolic contents and antioxidant activity were obtained from Alfa Aesar (Karlsruhe, Germany) and Sigma Aldrich (Steinheim, Germany). The solvents (methanol, acetonitrile, and formic acid) and reference standards of polyphenols (purity greater than 95%) for UHPLC analyses were purchased from Sigma Aldrich (Steinheim, Germany). All materials for antimicrobial activity tests (Nutrient agar—NA, Sabouraud dextrose agar—SDA, Müller–Hinton broth—MHB, and Sabouraud dextrose broth—SDB) were obtained from Torlak Institute of Virology, Vaccines and Sera (Belgrade, Serbia). Regarding the anti-inflammatory activity assays, the reagents and assay kits were purchased as follows: arachidonic acid, purified prostaglandin H synthase (PGHS)-1 from ram seminal vesicles, human recombinant COX-2, and NS-398 were purchased from Cayman Chemical Co. (Ann Arbor, MI, USA), Na2EDTA and tris(hydroxymethyl)-aminomethanhydro-chlorid (Titriplex III) from Merck KGaA (Darmstadt, Germany). Hematin and indomethacin (porcine) were obtained from ICN (Aurora, OH, USA), competitive PGE2 EIA kit from Enzo Life Sciences Inc. (Farmingdale, NY, USA), formic acid and DMSO (>99.98% purity) from Sigma-Aldrich (St. Louis, MO, USA), and epinephrine hydrogen tartarate from Fluka (St. Louis, MO, USA).
Katanić Stanković J.S., Đorović Jovanović J., Mišić D., Gašić U., Nikles S., Marković Z, & Bauer R. (2023). UHPLC-MS Phytochemical Profiling and Insight into Bioactivity of Rabelera holostea (Greater Stitchwort) Extract. Molecules, 28(3), 1274.
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Publication 2023
acetonitrile Agar Anti-Inflammatory Agents Antioxidant Activity Arachidonic Acid Biological Assay Caimans Cyclooxygenase-1 Dinoprostone Epinephrine formic acid Glucose Hematin Homo sapiens Hydrogen Indomethacin Methanol Microbicides NS 398 Nutrients Pigs Polyphenols PTGS2 protein, human Seminal Vesicles Serum Solvents Spectrophotometry Sulfoxide, Dimethyl Tromethamine Vaccines
4
Photodiode Reset Time Measurement
Each pixel unit contains a local reset switch controlled by the RST signal to connect the photodiode to the power supply VDD (Extended Data Fig. 8a). When the reset switch is turned on to enable the reset operation for the computing line, the photodiodes are charged to supply voltage VDD with the local charging paths in each pixel. The charging speed is determined by the RC time constant τ = RS0CPD, where CPD is the capacitance of the photodiode and RS0 is the on-resistance of the reset switch (Extended Data Fig. 8b). The transient function of the voltage of the photodiode with time can be formulized with the standard RC charging function as VPD(t) = VDD – (VDD – V0)e−t/τ, where V0 is the initial voltage of the photodiode. Theoretically, VPD approaches the stable-state-voltage VDD as time t approaches infinite. Here, we consider VPD reaching the stable state when the increase of VPD from V0 is larger than 99% of VDD – V0, and thus the reset time is derived as tr = 4.6τ, which is about 12 ns according to the post-simulation result (Extended Data Fig. 8d). The voltage of the computing line is read out with an on-chip buffer to the chip I/O pin and recorded by an oscilloscope. However, because of the limited bandwidth of the on-chip buffer, the output signal may be distorted when the computing line is charged at a high speed, affecting the precision of the measured reset time. To measure the reset time more precisely, we used peripheral charging paths instead of the in-pixel local charging paths for the reset operation. The 1,024 photodiodes in the pixel array were all connected to the computing line V+, and V+ was connected to the power supply VDD with 32 peripheral switches (Extended Data Fig. 8a,c). Thus, the RC time constant of the peripheral charging path becomes τ′ = (RS0/32) × (1,024 × CPD) = 32τ, resulting in the reset time of about 32 times 12 ns. The experimentally measured reset time with peripheral charging paths is presented in Fig. 6b. The horizontal dashed lines are the average values of the steady-state voltage. The vertical dashed lines are the intersection points of the signal with the steady-state voltages (horizontal lines). Furthermore, if we consider the charging resistance introduced by RS1, the reset time with peripheral charging paths is larger than 32 times that with local charging paths. Therefore, the time of dividing the measured 398.8 ns in Fig. 6b by 32—that is, 12.5 ns is the upper limit of the experimental reset time, according well with the post-simulation results with Cadence (Extended Data Fig. 8 and Supplementary Note 7).
Chen Y., Nazhamaiti M., Xu H., Meng Y., Zhou T., Li G., Fan J., Wei Q., Wu J., Qiao F., Fang L, & Dai Q. (2023). All-analog photoelectronic chip for high-speed vision tasks. Nature, 623(7985), 48-57.
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Publication 2023
12-nitroxide stearate Buffers DNA Chips NS 398 Transients
5
Modulating Spine Follicle Development
ATPase inhibitor (Blebbistatin, 1mM, Beyotime) and COX2 inhibitor (NS-398, 50 µM, Beyotime) were injected into the bottom of the spine follicle at the early growth phase using a microinjector, with DMSO as a control. Spine follicles were injected for two consecutive days and collected on the third and ninth days to observe their phenotypes using HE and immunofluorescence staining.
Zhang M., Wang M., Jiang J., Liu W., Zhou S., Wang D., Wang M., Zhao Z., Xu Z., Wu W., Lin X., Zhang J., Xu W., Tang Q., Zhan R., Liu W., Yang L., Zhou X., Zhou W, & Lei M. (2023). COX2-ATP Synthase Regulates Spine Follicle Size in Hedgehogs. International Journal of Biological Sciences, 19(15), 4763-4777.
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Publication 2023
Adenosine Triphosphatases blebbistatin Cyclooxygenase 2 Inhibitors Fluorescent Antibody Technique Hair Follicle NS 398 Ovarian Follicle Phenotype Sulfoxide, Dimethyl Vertebral Column

Top products related to «NS 398»

1
Ns 398 by Merck Group
Sourced in United States, Germany, Brazil
NS-398 is a selective cyclooxygenase-2 (COX-2) inhibitor. It is a laboratory research tool used to study the role of COX-2 in various cellular processes and disease models. The core function of NS-398 is to inhibit the COX-2 enzyme, which is involved in the production of pro-inflammatory prostaglandins.
2
Ns 398 by Cayman Chemical
Sourced in United States
NS-398 is a selective inhibitor of the cyclooxygenase-2 (COX-2) enzyme, which plays a key role in inflammation and pain processes. It is commonly used in research applications to investigate the role of COX-2 in various biological systems.
3
Indomethacin by Merck Group
Sourced in United States, Germany, United Kingdom, Brazil, Italy, Sao Tome and Principe, China, India, Japan, Denmark, Australia, Macao, Spain, France, New Zealand, Poland, Singapore, Switzerland, Belgium, Canada, Argentina, Czechia, Hungary
Indomethacin is a laboratory reagent used in various research applications. It is a non-steroidal anti-inflammatory drug (NSAID) that inhibits the production of prostaglandins, which are involved in inflammation and pain. Indomethacin can be used to study the role of prostaglandins in biological processes.
4
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Sc 560 by Merck Group
Sourced in Germany, United States, Sao Tome and Principe
The SC-560 is a laboratory equipment product manufactured by Merck Group. It is a device designed for specific laboratory functions, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach without extrapolation. Additional information about the intended use or capabilities of the SC-560 is not available.
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Sc 560 by Cayman Chemical
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The SC-560 is a laboratory centrifuge designed for general-purpose applications. It features a 6-place rotor with a maximum speed of 4,000 rpm and a maximum relative centrifugal force (RCF) of 2,800 x g. The centrifuge has a compact footprint and is suitable for a variety of sample preparation and separation tasks in research and clinical settings.
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Dimethyl sulfoxide (dmso) by Merck Group
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Rpmi 1640 medium by Thermo Fisher Scientific
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RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
9
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
10
Ns 398 by Bio-Techne
Sourced in United Kingdom
NS-398 is a selective inhibitor of the cyclooxygenase-2 (COX-2) enzyme. It is a laboratory research tool used for the study of COX-2 and its related biological processes.

More about "NS 398"

NS-398 is a highly selective and potent inhibitor of the cyclooxygenase-2 (COX-2) enzyme, which plays a critical role in inflammation and pain processes.
This compound has been extensively studied for its anti-inflammatory, analgesic, and potential antitumor properties, making it a valuable tool in biomedical research.
The COX-2 enzyme is known to be upregulated in various pathological conditions, including inflammation, pain, and cancer.
By selectively inhibiting COX-2, NS-398 can effectively reduce the production of proinflammatory prostaglandins, leading to a decrease in inflammation and pain.
This makes NS-398 a useful pharmacological agent in the study of inflammatory diseases, such as rheumatoid arthritis, osteoarthritis, and certain types of cancer.
In addition to its anti-inflammatory and analgesic effects, NS-398 has also been investigated for its potential antitumor properties.
Some studies have suggested that the inhibition of COX-2 by NS-398 may have a direct impact on tumor growth and development, making it a promising candidate for cancer research.
When working with NS-398, researchers often utilize other related compounds and materials, such as indomethacin (a non-selective COX inhibitor), fetal bovine serum (FBS) for cell culture, SC-560 (a selective COX-1 inhibitor), dimethyl sulfoxide (DMSO) as a solvent, and RPMI 1640 medium for cell culture.
The careful optimization and standardization of these research protocols can enhance the reproducibility and accuracy of NS-398-related studies.
PubCompare.ai's AI-driven platform can be a valuable tool for researchers working with NS-398.
By providing easy access to relevant literature, preprints, and patents, as well as AI-powered comparisons of different protocols and products, the platform can help researchers identify the best approaches and materials for their specific needs.
This can lead to improved reproducibility, accuracy, and overall efficiency in NS-398-related research.