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Oleate

Q: What are the key properties and functions of Oleate?

Oleate is a monounsaturated fatty acid found in various plant and animal oils. It plays a crucial role in biological processes, including lipid metabolism and signaling pathways. Oleate is extensively studied for its potential applications in diverse fields such as nutrition, biochemistry, and medicine.

Q: What are the different types or variations of Oleate?

Oleate can exist in different isomeric forms, such as cis-oleate and trans-oleate, which can have slightly different properties and biological effects. Additionally, oleate can be derived from various sources, including plant oils (e.g., olive oil, canola oil) and animal fats (e.g., dairy products, meat).

Q: What are some common usage challenges with Oleate?

One of the key challenges with Oleate is ensuring the reproducibility and accuracy of research protocols. Differences in Oleate sources, purity, and experimental conditions can lead to variablity in results. Researchers need to carefully optimize their protocols to account for these factors and ensure the reliability of their findings.

Oleate, a monounsaturated fatty acid found in various plant and animal oils, plays a crucial role in biological processes.
Involved in lipid metabolism and signaling pathways, oleate has been extensively studied for its potential applications in diverse fields, including nutrition, biochemistry, and medicine.
This MeSH term provides a concise overview of the chemical and functional properties of oleate, serving as a valuable resource for researchers exploring its role in health, disease, and scientific investigations.

Most cited protocols related to «Oleate»

Autophagy Regulation of Lipid Metabolism

Cited 60 times

The hepatocyte cell line RALA255-10G was cultured under nontransformed conditions, as described previously20 (link). Wild-type and Atg5^−/− mouse embryonic fibroblasts provided by N. Mizushima21 (link) were cultured as described previously22 (link). TG content was determined by the Trig/GB Kit (Roche Diagnostics), cholesterol content by the Amplex Red Cholesterol Assay (Invitrogen), fatty acid β-oxidation by a modification of a previously used method23 (link), and TG decay in cells radiolabelled with [¹⁴C]oleate and TG synthesis by standard methods12 (link). shRNAs were cloned into pSUPER (Ambion) and then pCCL.sin.PPT.hPGK.GFPWpre24 (link). Protein isolation and western blotting were performed as described previously25 (link). Fluorescence microscopy for BODIPY 493/503 (Invitrogen) and immunofluorescence were performed as described previously26 (link). Atg7^F/F mice4 (link) were crossed with Alb-Cre mice27 (link) to generate Atg7^F/F-Alb-Cre mice. Some animals were fed a high-fat diet (60% kcal in fat; Research Diets, D12492). Electron microscopy and immunogold labelling were performed as described previously26 (link). LDs from mouse livers were isolated by sucrose density gradient centrifugation28 and autophagic vacuoles and lysosomes by centrifugation in metrizamide discontinuous density gradients29 (link).

Singh R., Kaushik S., Wang Y., Xiang Y., Novak I., Komatsu M., Tanaka K., Cuervo A.M, & Czaja M.J. (2009). Autophagy regulates lipid metabolism. Nature, 458(7242), 1131-1135.

Publication 2009

4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene Anabolism Animals Autophagosome Biological Assay Cell Lines Cells Centrifugation Cholesterol Diagnosis Diet Diet, High-Fat Electron Microscopy Embryo Fatty Acids Fibroblasts formaldehyde-serum albumin Hepatocyte Immunofluorescence isolation Liver Lysosomes Metrizamide Mice, Laboratory Microscopy, Fluorescence Oleate Proteins Short Hairpin RNA Sucrose

Inactivated Mycobacterium bovis Vaccine Protocol

Cited 8 times

The M. bovis strain used was a first passage level culture isolated from a naturally infected wild boar in Coletsos medium. The isolate was propagated in Middlebrook 7H9 broth enriched with OADC for 2–3 weeks. Cells were harvested by centrifugation at 2500 x g for 20 minutes and washed twice in PBS. The bacterial pellet was re-suspended in PBS and declumped using a fine needle syringe. The turbidity of this suspension was adjusted to an optical density of 1 McFarland unit. Before inactivation, tenfold serial dilutions were prepared and plated in agar-solidified 7H9 with OADC in quadruplicate to assess the number of cfu in the inoculum. The inoculum was then inactivated in a water bath at 80°C for 30 minutes. Animals in “parenteral inactivated vaccine” and “oral inactivated vaccine” groups were administered with approximately 6×10⁶ bacteria according to cfu counts. The parenteral vaccine (1 ml) was prepared using Montanide ISA 50 V, an oily adjuvant of mannide oleate and mineral oil (Seppic, Castres, France). The oral vaccine consisted of 2 ml of PBS containing the inactivated mycobacteria. This inactivated vaccine was again cultured in duplicate to assure absence of viable M. bovis.

Garrido J.M., Sevilla I.A., Beltrán-Beck B., Minguijón E., Ballesteros C., Galindo R.C., Boadella M., Lyashchenko K.P., Romero B., Geijo M.V., Ruiz-Fons F., Aranaz A., Juste R.A., Vicente J., de la Fuente J, & Gortázar C. (2011). Protection against Tuberculosis in Eurasian Wild Boar Vaccinated with Heat-Inactivated Mycobacterium bovis. PLoS ONE, 6(9), e24905.

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Publication 2011

Agar Animals Bacteria Bath Cells Centrifugation montanide ISA 50 V Mycobacterium Needles Oil, Mineral Oils Oleate Parenteral Nutrition Pharmaceutical Adjuvants Strains Sus scrofa Syringes Technique, Dilution Vaccines Vaccines, Inactivated

Oleic Acid-Coated Magnetite Nanoparticles Synthesis

Cited 8 times

Oleic acid coated magnetite nanoparticles (NP-OA) were synthesized via thermal decomposition of iron oleate complex.[5 (link)] To a 5 ml of toluene solution containing 50 mg (iron content) of NP-OA, 40 mL of acetone was added, and the nanoparticles were collected by centrifugation. The nanoparticles were redispersed in 50 mL anhydrous toluene and transferred to a three-neck flask equipped with a heater. After the system was sealed and purged with nitrogen, 0.15 mL of SAS was injected, and the solution was heated to 100°C for 12 hours. The nanoparticles were precipitated by the addition of hexane, and collected using a rare earth magnet. The nanoparticles were washed twice with hexane and redispersed in anhydrous tetrahydrofuran (THF). To this solution, 100 mg of H₂NPEG-NH₂ and 2.5 mg of N,N′-dicyclohexylcarbodiimide (DCC) were added, and the reaction mixture was sonicated in a sonication bath for 12 hrs at 25°C. The nanoparticles was precipitated by the addition of 200 mL of hexane, and collected using a rare earth magnet. The precipitated nanoparticles were redispersed in 50 mL of anhydrous THF, and 250 mg of PEG-bis(amine) and 12.5 mg of DCC were added. The reaction mixture was kept in a sonication bath for 16 hrs at 25°C. The resulting product was precipitated by the addition of hexane, and collected using a rare earth magnet. After two additional cycles of THFredispersion and ether-precipitation, the residue solvent was evaporated and the nanoparticles were redispersed in 5 mL PBS. After passing through a 0.2 μm syringe filter, the nanoparticles were purified through gel filtration chromatography by Sephacryl S-200 column. The nanoparticles were stored in 0.1 M sodium bicarbonate buffer (pH 8.5). The concentration of nanoparticles was determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES).

Fang C., Bhattarai N., Sun C, & Zhang M. (2009). Functionalized Nanoparticles with Long-Term Stability in Biological Media. Small (Weinheim an der Bergstrasse, Germany), 5(14), 1637-1641.

Publication 2009

Acetone Amines Bath Bicarbonate, Sodium Buffers Centrifugation Dicyclohexylcarbodiimide Ethyl Ether Gel Chromatography Inductively Coupled Plasma Atomic Emission Spectroscopy Iron Magnetite Nanoparticles Metals, Rare Earth n-hexane Neck Nitrogen Oleate Oleic Acid Solvents Syringes tetrahydrofuran Toluene

Measurement of Macrophage Cholesterol Oleate

Cited 6 times

Adherent macrophages were incubated for 18 h with culture medium containing 10 μg/ml human oxidised LDL in the presence of 0.1 mmol/l [³H]oleate conjugated with BSA [13 (link), 14 (link)]. Cellular lipids were extracted and the radioactivity of cholesterol [³H]oleate was determined by thin-layer chromatography [13 (link), 14 (link)].

Nagashima M., Watanabe T., Terasaki M., Tomoyasu M., Nohtomi K., Kim-Kaneyama J., Miyazaki A, & Hirano T. (2011). Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice. Diabetologia, 54(10), 2649-2659.

Publication 2011

Cells Cholesterol Culture Media Homo sapiens LDL-1 Lipids Macrophage Oleate Radioactivity Thin Layer Chromatography

Synthesis and Characterization of Iron Oxide MNP Tracers

Cited 5 times

Three iron oxide MNP tracer samples, UW-1, UW-2, and LS-1 were used in phantom experiments reported here. Physical properties of the samples are listed in Table 1. Tracers were synthesized according to protocols described in previous work [31 (link)]. Synthesis protocols were based on thermolysis of iron (III) oleate with excess oleic acid in 1-octadecene. After synthesis, oleic acid coated MNPs were transferred from organic to aqueous phase using a PEG-ylated amphiphilic polymer [poly(maleic anhydride-alt-1 octadecene)-poly(ethylene glycol); PEG M_w ~ 5,000 Da (UW-1, UW-2) or 20,000 Da (LS-1)]. All three samples showed comparable magnetic behavior measured by MPS and vibrating sample magnetometer (VSM); the thicker polymer coating of sample LS-1 was designed to prolong blood circulation time for ongoing animal studies unrelated to this work. MNP magnetic size was determined by bright field TEM imaging (FEI, Hillsboro, OR) and by fitting a Langevin function to room-temperature magnetization curves (VSM, Lakeshore, Westerville, OH) to determine the median magnetic core diameter (d₀) and geometric standard deviation (exp(σ)), assuming the particle sizes satisfied a log normal distribution of the form

g (d_{C}) = \frac{1}{σ d_{C} \sqrt{2 π}} \exp - \frac{{[\ln (\frac{d_{C}}{d_{0}}]}^{2}}{2 σ^{2}},

where d_c is the magnetic core diameter [19 (link)]. The number-weighted diameter distribution was determined to enable direct comparison with TEM. For fitting M(H) data, the saturation magnetization, M_s, was determined from measured iron concentration; average M_s was 2.8×10⁵ A/m, (UW-1: 1.5×10⁵; UW-2: 3.7×10⁵; LS-1: 3.3×10⁵ A/m), similar to the measured M_s of Resovist^® (3.0×10⁵). Sample iron concentration was measured by inductively coupled plasma - optical emission spectrophotometer (ICP) (Perkin Elmer, Waltham, MA). Z-average hydrodynamic diameter, d_H, of aqueous-phase MNPs was determined by Dynamic Light Scattering (DLS) (Zetasizer, Malvern Instruments, Malvern, UK).

Ferguson R.M., Khandhar A.P., Kemp S.J., Arami H., Saritas E.U., Croft L.R., Konkle J., Goodwill P.W., Halkola A., Rahmer J., Borgert J., Conolly S.M, & Krishnan K.M. (2014). Magnetic Particle Imaging with Tailored Iron Oxide Nanoparticle Tracers. IEEE transactions on medical imaging, 34(5), 1077-1084.

Publication 2014

1-octadecene Anabolism Animals Blood Circulation Time ferric oxide Hydrodynamics Iron Maleic Anhydride Oleate Oleic Acid Physical Processes Plasma poly(ethylene glycol)diacrylate Poly A Polyethylene Glycols Polymers Resovist Vision

Most recents protocols related to «Oleate»

Withaferin A Attenuates NAFLD Progression

Withaferin A was purchased from Xenon Biosciences (India), and the chow diet was procured from Adita Biosys Pvt Ltd. The high-fat diet was procured from VRK Nutritional Solutions, India. Glucose and fructose were procured from Sisco Research Laboratories Pvt. Ltd. Commonly used liver function test enzymes, like aspartate transaminases or aspartate aminotransferase (AST), alanine transaminase or alanine aminotransferase (ALT), and alkaline phosphatase (ALP), and lipid molecules like total cholesterol, triglycerides (TG), and high-density lipoproteins (HDL) kits were purchased from Agape Diagnostics Ltd. TRIzol reagent, sodium palmitate, oleate and Oil Red O stain solution were purchased from Sigma Aldrich, St. Louis, Missouri, United States. cDNA synthesis and SYBR green kits were purchased from Thermo Fisher Scientific. A TGF-β1 ELISA kit was purchased from Krishgen Biosystems. HepG2 and Huh7 cells were purchased from NCCS Pune, India. The cell culture media Minimum Essential Medium Eagle (MEM), Ham DMEM/F-12, 1:1 mixture, bovine serum albumin, and hematoxylin were purchased from HiMedia, India. Fetal bovine serum (FBS) and antibiotics like penicillin/streptomycin were purchased from Gibco. 25-Hydroxycholesterol was a gift provided by Dr. Perumal Madan Kumar, CSIR-CFTRI, Mysuru. Taurochenodeoxycholic acid and deoxycholic acid were a gift from Dr. Ramprasad Talahalli, CSIR-CFTRI, Mysuru.

Shiragannavar V.D., Sannappa Gowda N.G., Puttahanumantharayappa L.D., Karunakara S.H., Bhat S., Prasad S.K., Kumar D.P, & Santhekadur P.K. (2023). The ameliorating effect of withaferin A on high-fat diet-induced non-alcoholic fatty liver disease by acting as an LXR/FXR dual receptor activator. Frontiers in Pharmacology, 14, 1135952.

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Publication 2023

25-hydroxycholesterol Alkaline Phosphatase Anabolism Aspartate Transaminase Cell Culture Techniques Cells Cholesterol Culture Media D-Alanine Transaminase Deoxycholic Acid Diagnosis Diet, High-Fat DNA, Complementary Eagle Enzyme-Linked Immunosorbent Assay Enzymes Fetal Bovine Serum Fructose Glucose Hematoxylin High Density Lipoproteins Lipids Liver Function Tests Oleate Penicillins Serum Albumin, Bovine Sodium Palmitate solvent red 27 Streptomycin SYBR Green I Taurochenodeoxycholic Acid TGF-beta1 Therapy, Diet Triglycerides trizol withaferin A Xenon

Inducing Steatosis in Liver Cells

Stock solutions of sodium palmitate (SP) and oleate (OA) (Sigma-Aldrich, United States) were prepared, as previously described (Römer et al., 2021 (link); Cao et al., 2012 (link)). Briefly, 100 μM of SP and OA were incubated for 30 min at 50 °C. Later, fatty acids were mixed with BSA in a culture medium (the fatty acid to BSA molar ratio was 4:1). To induce steatosis, HepG2 and Huh7 cells were exposed to SP and OA conjugated with fatty acid-free BSA. After incubation for 24 h, the cells were treated for 24 h with various concentrations of withaferin A (1, 2.5, and 5 μM). Cells used as controls were treated with fatty acid-free media containing ethanol as a vehicle.

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Publication 2023

Cells Culture Media Ethanol Fatty Acids Molar Nonesterified Fatty Acids Oleate Sodium Palmitate Steatohepatitis withaferin A

3D Culture Assay for Colony Growth

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Open the protocol to access the free full text link

Publication 2023

Acetylcysteine Antibiotics, Antitubercular Caimans Cells Growth Factor Lipids Low-Density Lipoproteins MK 2206 Oleate sotrastaurin

Lipase Hydrolytic Activity Assay

Lipase hydrolytic activity was assessed by monitoring the conversion of 4-methylumbelliferyl oleate (4-MUO) into 4-methylumbelliferone (4-MU). The enzymatic activity was determined by measuring the 4-MU fluorescence signal at 460 nm (after excitation at 355 nm) over time. A commercial reference inhibitor, orlistat, was used as the positive control, and citrate-phosphate buffer (0.1 M, pH 7.4) was used as the reaction buffer. A series of 4-MU dilutions in buffer was used to generate the standard curve to convert absorbance values into 4-MU concentrations. A 4 g/L solution of lipase in buffer was centrifuged at 5000× g and 10 °C for 10 min, then the supernatant was aliquoted. The final on-well concentration used in the assay was 250 mg/L. In each microplate well, 20 µL of test products diluted in a 10:90 (v:v) DMSO:water mixture (or 20 µL of this solvent mixture for the controls), 20 µL of lipase in buffer (or 20 µL of buffer for the blanks), and 110 µL of buffer were incubated at 37 °C in a microplate reader for 10 min. Then, 10 µL of 4-MUO substrate (48 µM in DMSO) was added to each well to initiate the reaction. Fluorescence was measured at 355 nm/460 nm (excitation/emission) using a FLUO Star Omega (BMG LabTech, Champigny sur Marne, France) 96-well microplate reader, thermostatically controlled at 37 °C, every 30 s for 15 min. The relative enzymatic activity was calculated using Equation (1). The IC₅₀ of each compound corresponded to the lowest concentration at which the lipase activity was halved.

Haguet Q., Le Joubioux F., Chavanelle V., Groult H., Schoonjans N., Langhi C., Michaux A., Otero Y.F., Boisseau N., Peltier S.L., Sirvent P, & Maugard T. (2023). Inhibitory Potential of α-Amylase, α-Glucosidase, and Pancreatic Lipase by a Formulation of Five Plant Extracts: TOTUM-63. International Journal of Molecular Sciences, 24(4), 3652.

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Publication 2023

Biological Assay Buffers Citrates enzyme activity Fluorescence FLUOS Hydrolysis Hymecromone Lipase Oleate Orlistat Phosphates Solvents Suby's G solution Sulfoxide, Dimethyl Technique, Dilution

Metabolic Regulation in Mouse Hepatocytes

AML-12 mouse hepatocytes were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma, Rehovot, Israel) supplemented with 10% fetal calf serum (FCS), 100 mg/L l-glutamine and 100 mg/L penicillin at 37 °C in 5% CO₂. Cells were seeded in 12-well tissue culture plates, and once the desired confluence was reached, cells were synchronized with a 1-h pulse of 1 mM dexamethasone (Sigma). After 1 h, the medium was replaced with either high glucose (25 mM) or high fructose (25 mM) medium or high fructose (25 mM) containing a fatty acid complex of oleate and palmitate (ratio of 2:1) mixed with 10% bovine serum albumin. The final concentration of the palmitate/oleate mixture was 1 mM. Following 24 h of incubation, the medium was replaced, and cells were harvested in triplicates per treatment per time-point every 6 h for an additional 24 h.

Tsameret S., Chapnik N, & Froy O. (2023). Differential Effect of Fructose in the Presence or Absence of Fatty Acids on Circadian Metabolism in Hepatocytes. Metabolites, 13(2), 138.

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Publication 2023

Cells Dexamethasone Eagle Fatty Acids Fetal Bovine Serum Fructose Glucose Glutamine Hepatocyte Mus Oleate Palmitate Penicillins Pulse Rate Serum Albumin, Bovine Tissues

Top products related to «Oleate»

Oleate by Merck Group

Sourced in United States, Sao Tome and Principe, China

Oleate is a laboratory reagent used in various biochemical and analytical applications. It serves as a surfactant, aiding in the solubilization and dispersion of lipophilic compounds. Oleate is a naturally occurring fatty acid derivative that can be used to facilitate interactions between aqueous and non-aqueous phases in experimental settings.

Palmitate by Merck Group

Sourced in United States, Germany, Macao, United Kingdom, Sao Tome and Principe, France, Japan, China

Palmitate is a type of laboratory equipment used for research and analysis purposes. It is a fatty acid compound that serves as a common precursor for various biological processes. Palmitate is utilized in various scientific applications, including cell culture studies, biochemical assays, and metabolic research. The core function of Palmitate is to provide a standardized and reliable source of this essential fatty acid for experimental and analytical purposes.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Oleic acid by Merck Group

Sourced in United States, Germany, China, Italy, Japan, France, India, Spain, Sao Tome and Principe, United Kingdom, Sweden, Poland, Australia, Austria, Singapore, Canada, Switzerland, Ireland, Brazil, Saudi Arabia

Oleic acid is a long-chain monounsaturated fatty acid commonly used in various laboratory applications. It is a colorless to light-yellow liquid with a characteristic odor. Oleic acid is widely utilized as a component in various laboratory reagents and formulations, often serving as a surfactant or emulsifier.

Bovine serum albumin (bsa) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico

Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.

14c oleate by PerkinElmer

Sourced in United Kingdom

[14C]oleate is a radioactive tracer compound containing the carbon-14 isotope. It is used in various research applications to study lipid metabolism and transport processes in biological systems.

Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica

DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.

Bodipy 493 503 by Thermo Fisher Scientific

Sourced in United States, Germany, Italy, China, United Kingdom, Japan, France, Switzerland, Belgium

BODIPY 493/503 is a fluorescent dye that can be used to stain neutral lipids and lipid droplets. It has an excitation maximum at 493 nm and an emission maximum at 503 nm.

Penicillin streptomycin by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia

Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

Dulbecco modified eagle medium (dmem) by Merck Group

Sourced in United States, Germany, United Kingdom, Italy, Japan, China, Sao Tome and Principe, France, Macao, Canada, Switzerland, Spain, Australia, Austria, Poland, India, Hungary, Israel, Brazil, Ireland, Czechia, Denmark, Sweden, Argentina, Finland, Cameroon

DMEM (Dulbecco's Modified Eagle's Medium) is a commonly used cell culture medium formulated to support the growth and maintenance of various cell lines. It provides a balanced salt solution and essential nutrients required for cell proliferation. DMEM is suitable for use in a wide range of cell culture applications.

What are the key properties and functions of Oleate?

What are the different types or variations of Oleate?

How can Oleate be used in research and applications?

Oleate has a wide range of applications in research and various industries. It is commonly used as a substrate in lipid metabolism studies, as a signaling molecule in cellular processes, and as a component in pharmaceutical and cosmetic formulations. Oleate's unique properties make it a valuable tool for researchers exploring topics related to nutrition, biochemistry, and medicine.

What are some common usage challenges with Oleate?

How can PubCompare.ai help with Oleate research?

PubComapre.ai allows you to screen protocol literature more efficiently and leverage AI to pinpoint critical insights. The platform can help researchers identify the most effective protocols related to Oleate for their specific research goals. PubCompare.ai's AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy, accelerating your Oleate research with confidence and precision.

More about "Oleate"

Oleate, a monounsaturated fatty acid found in various plant and animal oils, plays a crucial role in numerous biological processes.
Closely related to Palmitate, another important fatty acid, Oleate is involved in lipid metabolism and signaling pathways, making it a subject of extensive research in the fields of nutrition, biochemistry, and medicine.
Oleic acid, the main component of Oleate, is commonly used in cell culture media such as DMEM (Dulbecco's Modified Eagle Medium) and is often supplemented with Bovine Serum Albumin (BSA) or Fetal Bovine Serum (FBS) to provide a nutrient-rich environment for cell growth.
Radioactively labeled [14C]oleate is also utilized in scientific investigations to track the metabolic fate of this fatty acid.
Fluorescent dyes like BODIPY 493/503 are employed to visualize and quantify intracellular lipid droplets, which store Oleate and other lipids.
Additionally, the use of Penicillin/Streptomycin antibiotics helps maintain a sterile cell culture environment, ensuring the reliability and reproducibility of Oleate-related research.
By understanding the chemical properties, biological functions, and experimental applications of Oleate, researchers can optimize their investigations and leverage the latest advancements in areas such as lipid metabolism, cell signaling, and potential therapeutic interventions.
PubCompare.ai, an AI-powered platform, can assist in identifying the most effective and reproducible protocols, products, and research insights to accelerate your Oleate-focused studies.