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Oxalic Acid

Oxalic Acid: A naturally occurring dicarboxylic acid found in many plants.
It is an important industrial chemical with applications in various fields, including pharmaceuticals, agriculture, and materials science.
Researchers can leverage PubCompare.ai's cutting-edge AI-powered tools to efficiently locate the most reproducible and effective protocols for their Oxalic Acid studies, optimizing the research process and enhancing reproducibility.
Discover the power of AI-driven protocol comparision to advance your Oxalic Acid research.

Most cited protocols related to «Oxalic Acid»

Determination of 89Zr Specific Activity

Cited 23 times

Protocol full text hidden due to copyright restrictions

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Publication 2009

chelex Chlorides Deferoxamine Gamma Rays Ligands Oxalates Oxalic Acid Pentetic Acid Radiation Solvents Technique, Dilution Titrimetry Zirconium

Radiolabeling of Monoclonal Antibody J591 with Zirconium-89

Cited 12 times

The IgG₁ monoclonal antibody J591 was conjugated to the tris-hydroxamate, hexadentate chelate, desferrioxamine B (DFO, Calbiochem, Spring Valley, CA) by using a 6-step procedure modified from that described by Verel et al.(23 (link)) Full details are provided in the supporting information.
Zirconium-89 was produced via the ⁸⁹Y(p,n)⁸⁹Zr transmutation reaction on an EBCO TR19/9 variable beam energy cyclotron (Ebco Industries Inc., Richmond, British Columbia, Canada) in accordance with previously reported methods.(23 (link), 24 (link)) The [⁸⁹Zr]Zr-oxalate was isolated in high radionuclidic and radiochemical purity (RCP) >99.9%, with an effective specific-activity of 195–497 MBq/µg, (5.28–13.43 mCi/µg).(24 (link))
⁸⁹Zr-DFO-J591 was prepared by the complexation of [⁸⁹Zr]Zr-oxalate with DFO-J591. Typical radiolabeling reactions were conducted in accordance with the following procedure. Briefly, [⁸⁹Zr]Zr-oxalate (153.2 MBq, [4.14 mCi]) in 1.0 M oxalic acid (170 µL) was adjusted to pH7.7–8.1 with 1.0 M Na₂CO₃(aq.). CAUTION: Acid neutralization releases CO₂(g) and care should be taken to ensure that no radioactivity escapes the microcentrifuge vial. After CO₂(g) evolution ceased, DFO-J591 (400 µL, 2.1 mg/mL [0.84 mg of mAb], in 0.9% sterile saline) was added and the reaction was mixed gently by aspirating with a pipette. The reaction was incubated at room temperature for between 1–2 h and complexation progress was monitored with respect to time by ITLC (DTPA, 50 mM, pH7). After 1 h, crude radiolabeling yields and RCP was >95%. ⁸⁹Zr-DFO-J591 was purified by using either size-exclusion chromatography (Sephadex G-25 M, PD-10 column, >30 kDa, GE Healthcare; dead-volume = 2.5 mL, eluted with 200 µL fractions of 0.9% sterile saline) or spin-column centrifugation (4 mL total volume, >30 kDa, Amicon Ultra-4, Millipore, Billerica, MA; washed with 4×3 mL, 0.9% sterile saline). The radiochemical purity (RCP) of the final ⁸⁹Zr-DFO-J591 (>77% radiochemical yield; formulation: pH5.5–6.0; <500 µL; 0.9% sterile saline) was measured by both radio-ITLC and analytical size-exclusion chromatography (loading <0.74 MBq [20 µCi], ca. 5–10 µL aliquots) and was found to be >99% in all preparations. In the ITLC experiment ⁸⁹Zr-DFO-J591 and [⁸⁹Zr]Zr-DFO remain at the baseline (R_f = 0.0), whereas ⁸⁹Zr⁴⁺(aq.) ions and [⁸⁹Zr]Zr-DTPA elute with the solvent front (R_f = 1.0).

Holland J.P., Divilov V., Bander N.H., Smith-Jones P.M., Larson S.M, & Lewis J.S. (2010). 89Zr-DFO-J591 for immunoPET imaging of prostate-specific membrane antigen (PSMA) expression in vivo. Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 51(8), 1293-1300.

Publication 2010

Acids Biological Evolution Centrifugation Cyclotrons EBCO Gel Chromatography IgG1 Ions J591 monoclonal antibody Normal Saline Oxalates Oxalic Acid Patient Discharge Pentetic Acid Radioactivity Radioisotopes Radiopharmaceuticals sephadex G 25 Solvents Sterility, Reproductive Tromethamine Zirconium-89

Ascorbic Acid Content Quantification

Cited 9 times

Ascorbic acid content was determined using the methods of Sun et al.^{13 (link)} Fifty mg of sample powder was extracted with 5 mL 1.0% (w/v) oxalic acid, subsequently centrifuged 5 min at 4000g. Each sample was filtered through a 0.45 μm cellulose acetate filter. HPLC analysis of ascorbic acid was carried out using a Waters instrument with a Model 2996 PDA detector (Waters Inc., Milford, USA). Sample (20 μL) were separated at room temperature on a Waters Spherisorb C18 column (150 × 4.6 mm id; 5 μm particle size), using a solvent of 0.1% oxalic acid at a flow rate of 1.0 mL min⁻¹. The amount of ascorbic acid was calculated from absorbance values at 243 nm, using authentic ascorbic acid as a standard. The results were expressed as mg g⁻¹ dry weight.

Sun B., Tian Y.X., Jiang M., Yuan Q., Chen Q., Zhang Y., Luo Y., Zhang F, & Tang H.R. (2018). Variation in the main health-promoting compounds and antioxidant activity of whole and individual edible parts of baby mustard (Brassica juncea var. gemmifera). RSC Advances, 8(59), 33845-33854.

Publication 2018

acetylcellulose Ascorbic Acid High-Performance Liquid Chromatographies Oxalic Acid Powder Solvents

Cell-free Protein Expression Optimization

Cited 8 times

Refer to the detailed description for the preparation of the cell-free expression experiment included in the Supplemental Note and the Supplemental Experimental Design Spreadsheet. The final CFE reaction mixture is composed of the following reagents: 10–20 mM magnesium glutamate; 10 mM ammonium glutamate; 130 mM potassium glutamate; 1.2 mM ATP; 0.850 mM each of GTP, UTP, and CTP; 0.034 mg/mL folinic acid; 0.171 mg/mL yeast tRNA; 2 mM amino acids; 30 mM PEP; 0.33 mM NAD; 0.27 mM CoA; 4 mM oxalic acid; 1 mM putrescine; 1.5 mM spermidine; 57 mM HEPES; 30% CFE extract by volume; plasmid DNA to the desired concentration (refer to Table S1 for plasmid DNA concentrations used in this study); and water. For reactions involving T7 RNAP expression, in-house purified T7 RNAP was doped into the reaction at 0.10 mg/mL. The optimal magnesium concentration was determined for each reporter construct and was found to be 16 mM magnesium glutamate in nearly all cases (for exceptions to this, as well as the inducer concentrations in Figure 5, refer to Table S1).
All kinetic CFE reactions were prepared on ice in triplicate at the 10 μL scale. 33 μL of a mixture containing the desired reaction components was prepared and then 10 μL was pipetted into three wells of a 384-well plate (Corning, 3712), taking care to avoid bubbles. Plates were sealed (Thermo Scientific, 232701) and sfGFP fluorescence (emission/excitation: 485/520 nm) was monitored every 5 min on a BioTek Synergy Him plate reader for 8 h at 30 °C. For the bulk endpoint experiments in Figure 1, reactions were prepared to the 15 μL scale in triplicate, pipetted into the bottom of a 2.0 mL microcentrifuge tube, and incubated without shaking at 30 °C overnight for 15 h. Final protein titers were calculated from a previously developed plate reader correlation to either sfGFP (high-yield overnight experiments) or FITC (low-yield kinetic experiments) (see below). For the malachite green experiments in Figure 3, fluorescence (emission/excitation: 615/650 nm) was measured every 3 min. For all experiments, a no-DNA negative control was prepared in triplicate for every extract being tested. All reported fluorescence values have been baseline-subtracted by the no-DNA condition, and all error bars include the propagated error from the no-DNA condition.

Silverman A.D., Kelley-Loughnane N., Lucks J.B, & Jewett M.C. (2019). Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry. ACS synthetic biology, 8(2), 403-414.

Publication 2019

Amino Acids Ammonium Cells Dietary Fiber Fluorescein-5-isothiocyanate Fluorescence Glutamates HEPES Kinetics Leucovorin Magnesium malachite green Oxalic Acid Plasmids Potassium Glutamate Proteins Putrescine Spermidine Transfer RNA Yeast, Dried

Mycobacterium Tuberculosis Complex Identification

Cited 8 times

The sputum samples were decontaminated with 5% oxalic acid [16] (link), and inoculated on Lowenstein-Jensen Media (supplemented with either glycerol or pyruvate) slants, incubated at 37 °C until growth was observed. Colonies from positive cultures were sub-cultured on similar media and incubated as above until confluent growth was observed. The isolates (1490) were confirmed MTBC by PCR amplification of IS6110, genotyped as MTBSS, MAF and/or Mycobacterium bovis by large sequence polymorphism (LSPs) detecting region of difference (RD) 4, 9 and 12 [17] (link) and spoligotyping as described by Kamerbeek et al. [18] (link).

Otchere I.D., Asante-Poku A., Osei-Wusu S., Baddoo A., Sarpong E., Ganiyu A.H., Aboagye S.Y., Forson A., Bonsu F., Yahayah A.I., Koram K., Gagneux S, & Yeboah-Manu D. (2016). Detection and characterization of drug-resistant conferring genes in Mycobacterium tuberculosis complex strains: A prospective study in two distant regions of Ghana. Tuberculosis (Edinburgh, Scotland), 99, 147-154.

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Publication 2016

Culture Media Genetic Polymorphism Glycerin Mycobacterium bovis Oxalic Acid Pyruvate Sputum

Most recents protocols related to «Oxalic Acid»

Varenicline Oxalate Isolation from Base

Not available on PMC !

Example 19

A solution of Varenicline free base (20.0 g) in methylene dichloride (100 ml) was stirred with the aqueous solution of Oxalic acid (14.35 g, 1.2 eq in 100 ml of water). The aqueous layer containing Varenicline oxalate was stirred with methylene dichloride to remove the nitrosamine impurity by solvent extraction. Thereafter, follow the general procedure for the isolation of Varenicline base from the aqueous layer. Yield: 14.6 g.

US11872234B2. Vareniciline compound and process of manufacture thereof (2024-01-16). Par Pharmaceutical, Inc. [US]. Inventors: Joseph Prabahar Koilpillai [IN], Sayuj Nath [IN], Satish Patil [IN], Somasundaram Muthuramalingam [IN], Selvakumar Viruthagiri [IN], Mohankumar Lakshmanan [IN].

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Patent 2024

isolation Methylene Chloride Nitrosamines Oxalates Oxalic Acid Solvents Varenicline

Synthesis of Cis-Isomer Compound A4-Hemi-Oxalate

Not available on PMC !

Example 3

To a solution of compound (A3) (40.0 g, 92.5 mmol) and toluene (311 mL) was added at −10° C. a solution of NaHMDS in THF (93.2 mL, 185 mmol, 2 M) over a period of 7.5 minutes. A slight increase in temperature was observed. The reaction mixture was stirred at −10° C. for 50 minutes. Then the temperature was raised to 5° C. and kept there overnight. The reaction mixture was then allowed reach room temperature, and an aqueous solution of NaCl (160 g, 5% w/w) was added over a period of 5 minutes. The organic phase was separated and evaporated to dryness in vacuum. The residue was stripped with acetone (200 mL), and the residue was mixed with acetone and oxalic acid (8.30 g, 92.2 mmol) and stirred overnight at room temperature. The mixture was then cooled on ice bath for 1 hour and filtered. The filter cake was washed with cold acetone (2×50 mL) twice, broken up and dried in vacuum at 50° C. to yield compound (A4-hemi-oxalate) (35.0 g, 78%) as a powder, exclusively as the cis isomer as determined by ¹H NMR analysis.

LC-MS: RT=0.61 minutes, [M+H]⁺=396.3 m/z.

¹H NMR (600 MHz, DMSO-d₆) δ 7.07 (s, 1H), 7.03 (s, 1H), 6.06 (s, 2H), 3.73 (br s, 1H), 2.99-3.16 (m, 2H), 2.73-2.90 (m, 4H), 2.43 (br s, 1H), 1.88-1.96 (m, 1H), 1.60-1.80 (m, 3H), 1.45-1.56 (m, 2H), 1.35 (s, 9H), 0.81 (t, J=7.5 Hz, 3H).

US11866410B2. Process for the manufacturing of (6AR,10AR)-7-propyl-6,6A,7,8,9,10,10A,11-octahydro-[1,3]dioxolo[4′,5′:5,6]benzo[1,2-G]quinoline and (4AR, 10AR)-1-propyl-1,2,3,4,4A,5,10,10A-octahydro-benzo[G]quinoline-6,7-diol (2024-01-09). H. Lundbeck A/S [DK]. Inventors: Mikkel Fog Jacobsen [DK], Martin Juhl [DK], Kåre Søndergaard [DK].

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Patent 2024

1H NMR Acetone Bath Cold Temperature Fever Isomerism Oxalates Oxalic Acid Powder Sodium Chloride Sulfoxide, Dimethyl Toluene Vacuum

One-pot Synthesis of NVO/rGO Photocatalyst

The NH₄V₄O₁₀/rGO (NVO/rGO) photocatalyst was prepared via a facile one-pot hydrothermal method. NH₄VO₃ and GO were used as precursors and mixed with a weight ratio of 10:1. In brief, 0.6 g of NH₄VO₃ and 0.6 g of oxalic acid were dissolved in 90 ml of deionized water. In the meantime, 60 mg of GO was dispersed in 30 ml of deionized water with ultrasonication (20 W, 30 min). Next, the prepared reagents were mixed together and sonicated for 15 min. Then, the as-obtained reaction mixture was transferred to a Teflon-lined stainless-steel autoclave (volume 1.8 L) for 8 h and 180 °C. Finally, the obtained product was washed with deionized water and dried at 40 °C under reduced pressure (0.01 bar). For comparison, bare NH₄V₄O₁₀ (NVO) without GO was synthesized in an analogous procedure.

Nadolska M., Szkoda M., Trzciński K., Ryl J., Lewkowicz A., Sadowska K., Smalc-Koziorowska J, & Prześniak-Welenc M. (2023). New light on the photocatalytic performance of NH4V4O10 and its composite with rGO. Scientific Reports, 13, 3946.

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Publication 2023

Oxalic Acid Pressure Stainless Steel Teflon

Graphene Oxide Composite Synthesis

Ammonium metavanadate (NH₄VO_3, 99.0%), oxalic acid dihydrate (C₂H₂O₄ × 2H₂O, 97.0%), and methylene blue (MB > 98%) were obtained from Sigma‒Aldrich and used without further purification. Deionized water was used in all experiments (conductivity < 0,06 μS/cm). Graphene oxide (GO) employed in the composite synthesis was prepared using the modified Hummers method ^{69 (link)}. Potassium dichromate (K₂Cr₂O₇, ≥ 99.0%) and ammonium oxalate (AO, ≥ 99%) were purchased from Merck. Benzoquinone (BQ, > 98%) and tert-butyl alcohol (TBA, > 99.5%) were received from CheMondis.

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Publication 2023

1,4-benzoquinone ammonium metavanadate Ammonium Oxalate Anabolism Electric Conductivity graphene oxide Methylene Blue Oxalic Acid Potassium Dichromate tert-Butyl Alcohol

Heme Quantification Assay Protocol

A modified version of the fluorometry heme measurement assay was performed as described (88 (link), 89 (link)). Hundred microliters of 2 M oxalic acid were added to 100-μL samples of 16 μM-purified protein or 100-μL FLAG immunoprecipitation elutions from S. cerevisiae. The resulting 200-μL reaction was split into two replicate 100-μL tubes (samples arrayed in a strip tube). The first replicate tube was kept in the dark at RT, and the other replicate tube was incubated at 98 °C for 30 min in a Mastercycler X50s thermocycler (Eppendorf). Both replicate samples were transferred to a black-walled microplate, and fluorescence measurements (λ_ex = 400 nm; λ_em = 620 nm) were taken on an Infinite M1000 multimode microplate reader (Tecan). The resulting fluorescence data were exported to Microsoft Excel and analyzed in Prism 9.2.0 (GraphPad by Dotmatics). Unheated samples were subtracted from the heated samples to produce the final fluorescence values and reported in relative fluorescence units.

Schmitz J.M., Wolters J.F., Murray N.H., Guerra R.M., Bingman C.A., Hittinger C.T, & Pagliarini D.J. (2023). Aim18p and Aim46p are chalcone isomerase domain–containing mitochondrial hemoproteins in Saccharomyces cerevisiae. The Journal of Biological Chemistry, 299(3), 102981.

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Publication 2023

Biological Assay DNA Replication Fluorescence Fluorometry Heme Immunoprecipitation Oxalic Acid prisma Proteins

Top products related to «Oxalic Acid»

Oxalic acid by Merck Group

Sourced in United States, Germany, India, Poland, China, Belgium, United Kingdom, Italy

Oxalic acid is a chemical compound with the formula H2C2O4. It is a colorless crystalline solid that is highly soluble in water. Oxalic acid is commonly used in various industrial and laboratory applications.

Sodium hydroxide (naoh) by Merck Group

Sourced in Germany, United States, India, United Kingdom, Italy, China, Spain, France, Australia, Canada, Poland, Switzerland, Singapore, Belgium, Sao Tome and Principe, Ireland, Sweden, Brazil, Israel, Mexico, Macao, Chile, Japan, Hungary, Malaysia, Denmark, Portugal, Indonesia, Netherlands, Czechia, Finland, Austria, Romania, Pakistan, Cameroon, Egypt, Greece, Bulgaria, Norway, Colombia, New Zealand, Lithuania

Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.

Oxalic acid by Sinopharm

Sourced in China

Oxalic acid is a dicarboxylic acid that is widely used in various laboratory applications. It is a colorless crystalline solid with a sharp, acidic taste. Oxalic acid is a common reagent in analytical chemistry, and it is often used for tasks such as sample preparation, titration, and cleaning laboratory glassware.

Fastin elastin assay kit by Biocolor

Sourced in United Kingdom, Ireland

The Fastin elastin assay kit is a laboratory equipment used to quantify the amount of elastin present in a sample. It provides a reliable and accurate method for measuring elastin levels without interpretation or extrapolation.

Fastin elastin assay by Biocolor

Sourced in United Kingdom, Ireland, United States

The Fastin Elastin Assay is a laboratory kit designed to measure the amount of elastin present in a sample. The assay utilizes a specific dye to quantify the elastin content in a colorimetric manner. This product provides a reliable and standardized method for researchers to analyze the elastin levels in their samples.

Hydrochloric acid (hcl) by Merck Group

Sourced in Germany, United States, United Kingdom, India, Italy, France, Spain, Australia, China, Poland, Switzerland, Canada, Ireland, Japan, Singapore, Sao Tome and Principe, Malaysia, Brazil, Hungary, Chile, Belgium, Denmark, Macao, Mexico, Sweden, Indonesia, Romania, Czechia, Egypt, Austria, Portugal, Netherlands, Greece, Panama, Kenya, Finland, Israel, Hong Kong, New Zealand, Norway

Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.

Oxalic acid by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Belgium

Oxalic acid is a chemical compound with the formula H2C2O4. It is a colorless crystalline solid that is used as a reagent in various laboratory applications. Oxalic acid is a dicarboxylic acid and has a wide range of chemical and physical properties that make it useful for various analytical and experimental purposes in a laboratory setting.

Oxalic acid by PerkinElmer

Sourced in United States

Oxalic acid is a colorless, crystalline chemical compound with the formula C2H2O4. It is a dicarboxylic acid that is widely used in various industrial and laboratory applications.

Oxalic acid dihydrate by Merck Group

Sourced in United States

Oxalic acid dihydrate is a laboratory chemical compound that serves as a source of oxalic acid. It is a crystalline solid that is soluble in water and commonly used in various scientific applications.

Spherisorb c18 column by Waters Corporation

Sourced in Ireland, United States

The Spherisorb C18 column is a reversed-phase liquid chromatography column. It is designed for the separation and analysis of a variety of organic compounds. The column features a silica-based stationary phase with octadecyl (C18) functional groups, which provide a non-polar surface for the separation of analytes.

What is Oxalic Acid?

Oxalic Acid is a naturally occurring dicarboxylic acid found in many plants. It is an important industrial chemical with applications in various fields, including pharmaceuticals, agriculture, and materials science. Researchers can leverage PubCompare.ai's cutting-edge AI-powered tools to efficiently locate the most reproducible and effective protocols for their Oxalic Acid studies, optimizing the research process and enhancing reproducibility.

What are the common uses and applications of Oxalic Acid?

Oxalic Acid has a wide range of applications in various industries. It is used in the production of pharmaceuticals, as a cleaning agent, in the synthesis of other chemicals, and as a preservative in the food industry. Oxalic Acid is also used in agriculture as a herbicide and in the production of certain fertilizers. Additionally, it finds applications in materials science, where it is used in the production of certain types of dyes and pigments.

How can PubCompare.ai help with Oxalic Acid research?

PubCompare.ai allows you to screen protocol literature more efficiently and leverage AI to pinpoint critical insights. The platform can help researchers identify the most effective protocols related to Oxalic Acid for their specific research goals. The AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy in your Oxalic Acid studies.

What are the different variations or types of Oxalic Acid?

While Oxalic Acid is a single chemical compound, there are several variations or forms that can be used in research and applications. These include anhydrous Oxalic Acid, which is the pure, dry form, as well as hydrated forms like dihydrate and trihydrate. The choice of Oxalic Acid type can depend on the specific requirements of the research or application, such as solubility, stability, or reactivity.

What are some common challenges in working with Oxalic Acid?

One of the main challenges in working with Oxalic Acid is its toxicity. Oxalic Acid can be harmful if ingested or inhaled, and proper safety precautions must be taken when handling it. Additionally, Oxalic Acid can be corrosive to certain materials, so researchers must be mindful of the compatibility of equipment and containers. Proper storage and disposal of Oxalic Acid are also important considerations to ensure safe and efective use.

More about "Oxalic Acid"

Oxalic acid, also known as ethanedioic acid, is a naturally occurring dicarboxylic acid found in many plants and fungi.
It is an important industrial chemical with a wide range of applications, including pharmaceuticals, agriculture, and materials science.
Researchers can leverage PubCompare.ai's cutting-edge AI-powered tools to efficiently locate the most reproducible and effective protocols for their oxalic acid studies, optimizing the research process and enhancing reproducibility.
Oxalic acid is often used as a precursor for the production of sodium oxalate, a compound with various applications in the chemical industry.
Additionally, it can be used in the production of hydrochloric acid and other important chemicals.
The Fastin elastin assay kit is a common tool used to measure the elastin content in biological samples, which may be relevant in some oxalic acid research.
When working with oxalic acid, it is important to consider safety precautions, as it can be toxic in high concentrations.
Proper handling and storage, as well as the use of appropriate personal protective equipment, are essential.
Researchers may also need to consider the use of sodium hydroxide or other neutralizing agents to adjust the pH of solutions containing oxalic acid.
The Spherisorb C18 column is a type of high-performance liquid chromatography (HPLC) column that may be used for the separation and analysis of oxalic acid and related compounds in various samples.
Researchers can leverage PubCompare.ai's AI-powered tools to identify the most effective and reproducible protocols for using this and other analytical techniques in their oxalic acid studies.
By exploring the insights and tools provided by PubCompare.ai, researchers can optimize their oxalic acid research, enhance reproducibility, and drive scientific progress in this important field.