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Pentobarbital

Pentobarbital is a barbiturate drug used as a sedative-hypnotic, anticonvulsant, and general anesthetic.
It acts by enhancing the inhibitory effects of gamma-aminobutyric acid (GABA) in the central nervous system.
Pentobarbital is commonly used in research applications, such as animal studies and in vitro assays.
Optimizing the research process for Pentobarbital can be achieved through the use of AI-driven comparisons, which help locate the best protocols and products from literature, pre-prints, and patents, thus enhancing reproducibility and accracy.
The PubCompare.ai platform offers a powerful tool to harness this capability and streamline Pentobarbital research.

Most cited protocols related to «Pentobarbital»

1
Gliosis and Behavioral Consequences
Cited 41 times
All procedures followed the Institute of Laboratory Animal Research guidelines and were approved by the Animal Care and Use Committee of the National Institute of Mental Health. Transgenic mice expressing HSV-TK under the GFAP promoter were generated from a previously-generated plasmid28 (link) using standard techniques and bred on a mixed C57Bl/6:CD-1 background. Male v-WT and v-TK mice were treated with valganciclovir for 8 weeks (dexamethasone experiment), 10-19 weeks (endocrine), 12 weeks (behavior) or 4 weeks (histology; histology after 12 weeks in Supplementary Fig. 1), beginning at 8 weeks of age. Male C57Bl/6 mice were irradiated under pentobarbital anesthesia, as described previously29 (link), and tested 9 weeks later. For immunohistochemical analyses, mice were given BrdU 6 weeks (for PVN analysis) or 24 hours prior to sacrifice, brain sections were immunostained as previously described29 (link), and labeled cells were counted stereologically.
Serum corticosterone was measured by radioimmunoassay (MP Biomedicals) from submandibular blood samples obtained directly from the home cage condition or after exploration of a novel box, restraint, or isoflurane exposure. For the dexamethasone suppression test, dexamethasone (Sigma; 50 μg/kg in propylene glycol) or vehicle were injected 90 min prior to restraint, and blood was sampled immediately following 10 min restraint.
Behavioral tests were performed following 30 min of restraint or directly from the home cage. Different cohorts of mice were tested in the NSF test, elevated plus maze, forced swim test and sucrose preference test as previously described.12 (link), 18 (link), 21 , 30 (link) Statistical analyses were performed by t-test, log-rank test, or ANOVA with Fisher's LSD test for post hoc comparisons. Significance was set at P<0.05.
Snyder J.S., Soumier A., Brewer M., Pickel J, & Cameron H.A. (2011). Adult hippocampal neurogenesis buffers stress responses and depressive behavior. Nature, 476(7361), 458-461.
Publication 2011
Anesthesia Animals Animals, Laboratory Behavior Test BLOOD Brain Bromodeoxyuridine Cells Corticosterone Dexamethasone Elevated Plus Maze Test Glial Fibrillary Acidic Protein Isoflurane Males Mice, Inbred C57BL Mice, Laboratory Mice, Transgenic neuro-oncological ventral antigen 2, human Pentobarbital Propylene Glycol Radioimmunoassay Serum Sucrose System, Endocrine Valganciclovir
2
Stereotaxic Lentiviral Transduction for Hippocampal Neuromodulation
Cited 27 times

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Publication 2013
Animal Care Committees Animals Animals, Laboratory Antibodies Brain Cannula GRIN2A protein, human Immunohistochemistry Lentivirinae Males Memory Mice, House Mice, Knockout Microscopy, Fluorescence paraform Pentobarbital Rabbits Seahorses Sucrose Technique, Dilution
3
SARS-CoV-2 Transmission Dynamics in Hamsters
Cited 20 times
Male golden Syrian hamsters at 4–5 weeks old were obtained from Laboratory Animal Services Centre, Chinese University of Hong Kong. The hamsters were originally imported from Harlan (Envigo), UK in 1998. All experiments were performed at the BSL-3 core facility, LKS Faculty of Medicine, The University of Hong Kong. The animals were randomized from different litters into experimental groups, and the animals were acclimatized at the BSL3 facility for 4–6 days prior to the experiments. The study protocol have been reviewed and approved by the Committee on the Use of Live Animals in Teaching and Research, The University of Hong Kong (CULATR # 5323–20). Experiments were performed in compliance with all relevant ethical regulations. For challenge studies, hamsters were anesthetized by ketamine(150mg/kg) and xylazine (10mg/kg) via intra-peritoneal injection and were intra-nasally inoculated with 8 × 104 TCID50 of SARS-CoV-2 in 80 μL DMEM. On days 2, 5, 7, three hamsters were euthanized by intra-peritoneal injection of pentobarbital at 200mg/kg. No blinding was done and a sample size of three animals was selected to assess the level of variation between animals. Lungs (left) and one kidney were collected for viral load determination and were homogenized in 1mL PBS. Brain, nasal turbinate, lungs (right, liver, heart, spleen, duodenum, and kidney were fixed in 4% paraformaldehyde for histopathological examination. To collect fecal samples, hamsters were transferred to a new cage one day in advance and fresh fecal samples (10 pieces) were collected for quantitative real-time RT-PCR and TCID50 assay. To evaluate SARS-CoV-2 transmissibility by direct contact, donor hamsters were anesthetized and inoculated with 8 × 104 TCID50 of SARS-CoV-2. On 1 dpi or on 6 dpi, one inoculated donor was transferred to co-house with one naïve hamster in a clean cage and co-housing of the animals continued for at least 13 days. Experiments were repeated with three pairs of donors: direct contact at 1:1 ratio31 (link),32 (link). Body weight and clinical signs of the animals were monitored daily. To evaluate SARS-CoV-2 transmissibility via aerosols, one naïve hamster was exposed to one inoculated donor hamster in two adjacent stainless steel wired cages on 1 dpi for 8 hours (Extended Data Fig. 3). DietGel®76A (ClearH2O®) was provided to the hamsters during the 8-hour exposure. Exposure was done by holding the animals inside individually ventilated cages (IsoCage N, Techniplast) with 70 air changes per hour. Experiments were repeated with three pairs of donors: aerosol contact at 1:1 ratio. After exposure, the animals were single-housed in separate cages and were continued monitored for 14 days. To evaluate transmission potential of SARS-CoV-2 virus via fomites, three naïve fomite contact hamsters were each introduced to a soiled donor cage on 2 dpi. The fomite contact hamsters were single-housed for 48 hours inside the soiled cages and then were each transferred to a new cage on 4 dpi of the donor. All animals were continued monitored for 14 days. For nasal wash collection, hamsters were anesthetized by ketamine (100mg/kg) and xylazine (10mg/kg) via intra-peritoneal injection and 160 μL of PBS containing 0.3% BSA was used to collect nasal washes from both nostrils of each animal. Collected nasal washes were diluted 1:1 by volume and aliquoted for TCID50 assay in Vero E6 cells and for quantitative real-time RT-PCR. The contact hamster were handled first followed by surface decontamination using 1% virkon and handling of the donor hamster.
Sia S.F., Yan L.M., Chin A.W., Fung K., Choy K.T., Wong A.Y., Kaewpreedee P., Perera R.A., Poon L.L., Nicholls J.M., Peiris M, & Yen H.L. (2020). Pathogenesis and transmission of SARS-CoV-2 in golden Syrian hamsters. Nature, 583(7818), 834-838.
Publication 2020
Animals Animals, Domestic Animals, Laboratory Biological Assay Body Weight Brain Chinese Decontamination Donors Duodenum Faculty, Medical Feces Fomites Hamsters Heart Injections, Intraperitoneal Ketamine Kidney Liver Lung Males Mesocricetus auratus Nose paraform Patient Holding Stretchers Pentobarbital Real-Time Polymerase Chain Reaction SARS-CoV-2 Spleen Stainless Steel Tissue Donors Transmission, Communicable Disease Turbinates Vero Cells Virkon Xylazine
4
Isolation and Culture of Neuronal and Cell Lines
Cited 18 times
Rat pheochromocytoma (PC12) and SH-SY5Y cell lines were purchased from American Type Culture Collection (ATCC) (Manassas, VA). PC12 cells were used for no more than 10 passages, and were in antibiotic-free DMEM supplemented with 10% horse serum and 5% FBS, whereas SH-SY5Y cells were grown in antibiotic-free DMEM supplemented with 10% FBS. Cells were maintained in a humid incubator (37°C, 5% CO2).
To isolate primary neurons, CD-1 mice were purchased from Charles River Laboratories (Wilmington, MA) and housed in the Animal Resource Center at Louisiana State University Health Sciences Center-Shreveport under specific pathogen-free conditions. All procedures were carried out in accordance with the guidelines of the Institutional Animal Care and Use Committee and were in compliance with the guidelines set forth by the Guide for the Care and Use of Laboratory Animals. Fetal mouse cerebral cortexes of 14-18 days of gestation were used. Briefly, the pregnant mice were anaesthetized with pentobarbital and embryos were removed by a cesarean section under sterile conditions. Cerebral cortexes were isolated under dissection microscope and washed with ice-cold Ca2+/Mg2+-free Hank's buffered salt solution (HBSS) supplemented with 1% glucose and 50 μg/ml gentamicin. The isolated embryo cortexes were digested with papain solution [HBSS containing 500 μg/ml papain, 50 μg/ml DNase1, 1% glucose, 10 μM CaCl2, 5 mM EDTA] for 15 min at 37°C. Digested tissues were triturated into single cells using 1 ml pipette in NEUROBASAL™ Media supplemented with 2% B27 Supplement, 2 mM glutamine, 1 mM sodium pyruvate, 5 μg/ml insulin, and 40 μg/ml of gentamicin. Isolated cells were seeded at a density of 2 × 106 cells/well in a 6-well plate coated with 10 μg/ml PDL in the above culture medium, and grown in a humid incubator (37°C, 5% CO2). Fresh medium was replaced every 3 days. The cells were used for experiments after 6 days of culture.
Chen L., Xu B., Liu L., Luo Y., Yin J., Zhou H., Chen W., Shen T., Han X, & Huang S. (2010). Hydrogen peroxide inhibits mTOR signaling by activation of AMPKα leading to apoptosis of neuronal cells. Laboratory investigation; a journal of technical methods and pathology, 90(5), 762-773.
Publication 2010
Animals Animals, Laboratory Antibiotics Cell Lines Cells Cesarean Section Common Cold Cortex, Cerebral Dietary Supplements Dissection Edetic Acid Embryo Equus caballus Fetus Gentamicin Glucose Glutamine Institutional Animal Care and Use Committees Insulin Microscopy Mus Neurons Papain PC12 Cells Pentobarbital Pheochromocytoma Pregnancy Pyruvate Rivers Serum Sodium Sodium Chloride Specific Pathogen Free Sterility, Reproductive Tissues
5
Hyperinsulinemic-Euglycemic Clamp in Mice
Cited 15 times
Clamps were performed according to recent recommendations of the Mouse Metabolic Phenotyping Center Consortium (15 (link)). After surgical implantation of an indwelling catheter in the right jugular vein, the mice were allowed to recover for 1 week prior to clamp experiments. Following an overnight 14-h fast, the mice were infused with 3-[3H]glucose at a rate of 0.05 μCi/min for 120 min to determine basal glucose turnover. Next, a primed infusion of insulin and 3-[3H]glucose was administered at 7.14 milliunits·kg−1·min−1 and 0.24 μCi/min, respectively, for 4 min, after which the rates were reduced to 3 milliunits·kg−1·min−1 insulin and 0.1 μCi/min 3-[3H]glucose for the remainder of the experiment. Blood was collected via tail massage for plasma glucose, insulin, and tracer levels at set time points during the 140-min infusion, and a variable infusion of 20% dextrose was given to maintain euglycemia. Glucose turnover was calculated as the ratio of the 3-[3H]glucose infusion rate to the specific activity of plasma glucose at the end of the basal infusion and during the last 40 min of the hyperinsulinemic-euglycemic clamp study. Hepatic glucose production represents the difference between the glucose infusion rate and the rate of glucose appearance. A 10-μCi bolus injection of [14C]2-deoxyglucose was given at 90 min to determine tissue-specific glucose uptake, which was calculated from the area under the curve of [14C]2-deoxyglucose detected in plasma and the tissue content of [14C]2-deoxyglucose-6-phosphate, as previously described (16 (link)). Following collection of the final blood sample, the mice were anesthetized with an intravenous injection of 150 mg/kg pentobarbital, and tissues were harvested and froze with aluminum forceps in liquid nitrogen. All of the tissues were stored at −80 °C until later use.
Jurczak M.J., Lee A.H., Jornayvaz F.R., Lee H.Y., Birkenfeld A.L., Guigni B.A., Kahn M., Samuel V.T., Glimcher L.H, & Shulman G.I. (2011). Dissociation of Inositol-requiring Enzyme (IRE1α)-mediated c-Jun N-terminal Kinase Activation from Hepatic Insulin Resistance in Conditional X-box-binding Protein-1 (XBP1) Knock-out Mice. The Journal of Biological Chemistry, 287(4), 2558-2567.
Publication 2011
2-Deoxyglucose 2-deoxyglucose-6-phosphate Aluminum BLOOD Euglycemic Clamp Forceps Freezing Glucose Indwelling Catheter Insulin Jugular Vein Massage Mus Nitrogen Operative Surgical Procedures Ovum Implantation Pentobarbital Plasma Specimen Collections, Blood Tail Tissues Tissue Specificity

Most recents protocols related to «Pentobarbital»

1
Equine Intestinal Ischemia-Reperfusion Protocol
General anesthesia was induced with 0.1 mg/kg BW diazepam (Ziapam 5 mg/kg, Ecuphar GmbH, Greifswald, Germany) and 2.2 mg/kg BW ketamine (Narketan, Vétoquinol GmbH, Ismaning, Germany) after premedication with 0.7 mg/kg BW xylazine (Xylavet 20 mg/ml, CP-Pharma GmbH, Burgdorf, Germany). Anesthesia was maintained with isoflurane (Isofluran CP, CP-Pharma GmbH) in 100% oxygen, and continuous rate infusions with lactated Ringer's solution (Ringer-Laktat EcobagClick, B. Braun Melsungen AG, Melsungen, Germany) and dobutamine (Dobutamin-ratiopharm 250 mg, Ratiopharm GmbH, Ulm, Germany) were given to effect, to maintain the mean arterial blood pressure between 60 and 80 mmHg. A routine pre-umbilical median laparotomy was performed in dorsal recumbency following aseptic preparation. Segmental small intestinal ischemia was induced in 1.5 m jejunum by occlusion of the mesenteric vessels with umbilical tape. The ligature was tightened under monitoring of intestinal microperfusion with microlightguide spectophotometry and laser Doppler flowmetry (O2C, LEA Medizintechnik GmbH, Giessen, Germany), and the ligature was tied when the blood flow was reduced by 90% of the pre-ischemic measurement. The ischemia was maintained for 90 min. In group C, the ligature was released without manipulation of the vessels and reperfusion was initiated without delay. In group IPoC, postconditioning was implemented after release of ischemia by clamping the mesenteric vessels for three cycles of 30 s, alternated with 30 s of reperfusion. This was followed by 120 min of reperfusion in both groups. Subsequently, the horses were euthanized with 90 mg/kg BW pentobarbital intravenously (Release 50 mg/mL, WDT eG, Garbsen, Germany) without regaining consciousness.
Verhaar N., de Buhr N., von Köckritz-Blickwede M., Dümmer K., Hewicker-Trautwein M., Pfarrer C., Dengler F, & Kästner S. (2023). Hypoxia signaling in the equine small intestine: Expression and distribution of hypoxia inducible factors during experimental ischemia. Frontiers in Veterinary Science, 10, 1110019.
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Publication 2023
Anesthesia Asepsis Blood Circulation Blood Vessel Consciousness Diazepam Dobutamin-ratiopharm Dobutamine Equus caballus General Anesthesia Intestines Intestines, Small Ischemia Isoflurane Jejunum Ketamine Lactated Ringer's Solution Laparotomy Laser-Doppler Flowmetry Ligature Mesenteric Vascular Occlusion Mesentery Oxygen Pentobarbital Premedication Reperfusion Umbilicus Xylazine
2
Ethical Mouse Handling Protocols
All procedures involving the handling of living mice were performed in strict accordance with the German Animal Protection Law, and were approved by the Regional Office for Health and Social Services in Berlin (Landesamt für Gesundheit und Soziales, Berlin, Germany, Permit Number G0164/19, X9005/18, A0376/17). Adult mice were euthanized by cervical dislocation or by transcardial perfusion of PBS or PFA after intraperitoneal injection of pentobarbital (Narcoren, Merial GmbH, Hallbergmoos, Germany). All efforts were made to minimize suffering. Similarly, all mice used at Washington University were performed under an approved Animal Studies protocol.
Logiacco F., Grzegorzek L.C., Cordell E.C., Popp O., Mertins P., Gutmann D.H., Kettenmann H, & Semtner M. (2023). Neurofibromatosis type 1-dependent alterations in mouse microglia function are not cell-intrinsic. Acta Neuropathologica Communications, 11, 36.
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Publication 2023
Adult Animals Injections, Intraperitoneal Joint Dislocations Mice, House Neck Pentobarbital Perfusion
3
Immunohistochemical Analysis of Mouse Brain
Mice were anesthetized with pentobarbital (Narcoren, Merial Hallbergmoos, Germany) and transcardially perfused with phosphate buffered salt solution (PBS) followed by 4% paraformaldehyde in PBS, decapitated and sectioned in the sagittal plane at 40 μm thickness using a sliding microtome (Leica SM2000 R, Leica Biosystems GmbH, Nussloch, Germany). Free-floating 40 μm sections were incubated in 5% donkey serum (EMD Millipore Corp., Burlington, Massachusetts, USA) and 0.1% Triton-X (Carl Roth®, Karlsruhe, Germany) in Tris-buffered saline solution (TBSplus) together with the primary antibodies over-night at 4 °C. The following primary antibodies were used: goat monoclonal Iba-1 antibody targeting microglia (1:300; Abcam, Cambridge, UK); chicken polyclonal GFP antibody targeting eYFP (1:250; Abcam, Cambridge, UK); mouse anti-NeuN targeting neurons (1:00; Synaptic Systems). After washing, secondary antibodies were prepared in TBSplus. Iba-1 was visualized with donkey anti-goat IgG conjugated with Cy5 or Alexa488 fluorophores (both 1:200; Dianova, Hamburg, Germany); eYFP was visualized with donkey anti-chicken IgY conjugated with Alexa488 or Cy5 (both 1:200; Dianova), NeuN with donkey anti-mouse IgG (H + L) conjugated with Cy3 (1:200; Dianova). Sections were incubated with secondary antibodies at room temperature for 2 h and then mounted on glass slides with Aqua Polymount mounting medium (Polysciences Europe GmbH, Hirschberg an der Bergstraße, Germany). Cell nuclei were stained using 4',6-diamidino-2-phenylindole (DAPI, 1:500; Dianova) before mounting. Images were acquired with either a Zeiss LSM700 (inverse) or a Leica SPE (upright; Leica Biosystems GmbH, Nussloch, Germany) using 40X oil immersion objectives. Z-stacks were taken at 1 μm z-step size, 35 steps to cover the whole thickness of the slice.
Logiacco F., Grzegorzek L.C., Cordell E.C., Popp O., Mertins P., Gutmann D.H., Kettenmann H, & Semtner M. (2023). Neurofibromatosis type 1-dependent alterations in mouse microglia function are not cell-intrinsic. Acta Neuropathologica Communications, 11, 36.
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Publication 2023
anti-IgG Antibodies Cell Nucleus Chickens DAPI Equus asinus Goat Immunoglobulins Microglia Microtomy Monoclonal Antibodies Mus Neurons paraform Pentobarbital Phosphates Saline Solution Serum Sodium Chloride Submersion
4
Autologous Tendon Graft Transplantation
Intraoperative 3% pentobarbital injection (1.0 mL/kg) was first used for anesthesia via intraperitoneal injection and the skin of lower limbs was shaved and sterilized. Then, a harvest of ipsilateral flexor digitorum longus tendons was completed (from the lateral aspect of ankle joints) and muscles on harvested grafts were removed. The knee was exposed through medial parapatellar arthrotomy and a lateral dislocation of the patella exposed native ACL. A careful excision of the native ACL was completed and confirmed after the tibia was translated anteriorly. After the knee was flexed (90°), tibial and femoral tunnels (diameter: 1.5 mm, length:7 mm) were created by 1.5 mm diameter Kirschner wire starting from the original ACL footprint to the tibia’s medial side (tibial tunnel) or the femoral condyle’s anterolateral side (femoral tunnel) (Lui et al., 2014 (link)). A 4-0 Ethibond (Ethicon) was used to attach one side of the graft and drag it into the tunnel. Previously stored at 4° FHE (or FHE + BP) were injected into tunnels before grafts’ immediate placement (dragging). The knee joint was flexed to 30° and 4N graft pretention was applied before the suturing of grafts to the surrounding periosteum at both tibial and femoral ends was completed. Layered wound closure was then carried and the anterior stability of the knee was validated by Lachman test. Intramuscular anti-infectious injections (penicillin, 50 KU/kg) were given to animals before returning to cages and being allowed free movement.
Cho E., Qiao Y., Chen C., Xu J., Cai J., Li Y, & Zhao J. (2023). Injectable FHE+BP composites hydrogel with enhanced regenerative capacity of tendon-bone interface for anterior cruciate ligament reconstruction. Frontiers in Bioengineering and Biotechnology, 11, 1117090.
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Publication 2023
Anesthesia Animals Anti-Infective Agents Condyle Ethibond Femur Grafts Injections, Intraperitoneal Intramuscular Injection Joints, Ankle Kirschner Wires Knee Knee Joint Lower Extremity Movement Muscle Tissue Patellar Dislocation Penicillins Pentobarbital Periosteum Skin Tendons Tibia Wounds
5
Bone Regeneration with Titanium Implants

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Publication 2023
Alloys Animal Ethics Committees Animals Condyle Epistropheus Ethics Committees, Research Fascia Femur fluorexon Injections, Intraperitoneal Joint Dislocations Males Muscle Tissue Neck Osteogenesis Oxide, Ferrosoferric Pentobarbital Rattus norvegicus Skin Specific Pathogen Free Thigh Titanium

Top products related to «Pentobarbital»

1
Pentobarbital by Merck Group
Sourced in United States, Germany, China, Japan, Canada
Pentobarbital is a barbiturate compound used as a sedative and hypnotic agent in laboratory research and clinical settings. It acts as a central nervous system depressant. Pentobarbital is commonly used in medical and scientific applications, such as anesthesia, seizure control, and as a euthanasia agent for animals.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Paraformaldehyde (pfa) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, France, Macao, Australia, Canada, Sao Tome and Principe, Japan, Switzerland, Spain, India, Poland, Belgium, Israel, Portugal, Singapore, Ireland, Austria, Denmark, Netherlands, Sweden, Czechia, Brazil
Paraformaldehyde is a white, crystalline solid compound that is a polymer of formaldehyde. It is commonly used as a fixative in histology and microscopy applications to preserve biological samples.
4
Vt1000 by Leica
Sourced in Germany, United States, France, United Kingdom, Israel, Japan, Switzerland, Canada, Belgium
The VT1000S is a vibratome, a precision instrument used for sectioning biological samples, such as tissues or organs, into thin slices for microscopic examination or further processing. The VT1000S provides consistent and accurate sectioning of samples, enabling researchers to obtain high-quality tissue sections for a variety of applications.
5
Fatal plus by Vortech Pharmaceuticals
Sourced in United States
Fatal Plus is a specialized laboratory instrument designed for performing precise chemical analyses. It features advanced detection capabilities and is capable of accurately measuring a wide range of chemical compounds. The core function of Fatal Plus is to provide researchers and scientists with a reliable tool for conducting in-depth analysis of various substances.
6
Somnopentyl by Kyoritsu Seiyaku
Sourced in Japan
Somnopentyl is a laboratory instrument designed for the measurement and analysis of sleep-related parameters. It is a specialized device used in sleep research and clinical sleep studies to gather data on various sleep-related physiological indicators.
7
Pentobarbital by Kyoritsu Seiyaku
Sourced in Japan
Pentobarbital is a barbiturate compound commonly used as a sedative and hypnotic drug. It is a powder substance that can be dissolved in water for various laboratory applications. Pentobarbital has a depressant effect on the central nervous system.
8
Cryostat by Leica
Sourced in Germany, United States, Japan, United Kingdom, France, Australia, Canada, Denmark, Italy, Singapore, Switzerland, Israel
The Cryostat is a specialized piece of laboratory equipment used for the sectioning of frozen tissue samples. It maintains a controlled low-temperature environment, enabling the precise and consistent cutting of delicate specimens for microscopic analysis and examination.
9
Lipopolysaccharides (lps) by Merck Group
Sourced in United States, Germany, China, United Kingdom, Sao Tome and Principe, Macao, Italy, Japan, Canada, France, Switzerland, Israel, Australia, Spain, India, Ireland, Brazil, Poland, Netherlands, Sweden, Denmark, Hungary, Austria, Mongolia
The LPS laboratory equipment is a high-precision device used for various applications in scientific research and laboratory settings. It is designed to accurately measure and monitor specific parameters essential for various experimental procedures. The core function of the LPS is to provide reliable and consistent data collection, ensuring the integrity of research results. No further details or interpretations can be provided while maintaining an unbiased and factual approach.
10
Vt1200 by Leica
Sourced in Germany, United States, United Kingdom, Japan, Australia, Canada, Israel, Switzerland
The VT1200S is a vibrating microtome designed for precision sectioning of biological samples. It features a high-precision feed system and a stable base for consistent, uniform sectioning.

More about "Pentobarbital"

Pentobarbital, also known as Nembutal, Pentobarbitone, or Somnopentyl, is a powerful sedative-hypnotic drug that belongs to the barbiturate class.
It is commonly used in research applications, such as animal studies and in vitro assays, due to its potent anesthetic and anticonvulsant properties.
Pentobarbital works by enhancing the inhibitory effects of gamma-aminobutyric acid (GABA) in the central nervous system, leading to a depressed state of consciousness and reduced neuronal excitability.
This makes it a valuable tool for researchers studying the mechanisms of sleep, seizures, and other neurological processes.
In addition to its research applications, Pentobarbital has also been used in the past as a general anesthetic and for the euthanasia of animals, though its use for these purposes has become more restricted in recent years.
Other barbiturate drugs, such as Phenobarbital and Secobarbital, share similar properties and may be used in similar research contexts.
When conducting research with Pentobarbital, it is important to carefully follow established protocols and safety guidelines to ensure the accuracy and reproducibility of experiments.
The use of AI-driven comparison tools, such as PubCompare.ai, can help researchers identify the most effective and reliable protocols and products from the scientific literature, preprints, and patents, thereby enhancing the quality and efficiency of their Pentobarbital-based research.
Additionally, the use of complementary reagents and equipment, such as Fetal Bovine Serum (FBS), Paraformaldehyde, Cryostats, and Lipopolysaccharide (LPS), may be necessary for various research applications involving Pentobarbital.
By understanding the role and proper use of these related materials, researchers can further optimize their Pentobarbital-based studies and ensure the integrity of their findings.
In conclusion, Pentobarbital is a versatile and powerful tool for researchers, but its use requires careful planning and execution.
By leveraging AI-driven comparison platforms and understanding the broader context of related research materials, scientists can streamline their Pentobarbital-based workflows, enhance reproducibility, and advance their understanding of the complex mechanisms underlying the actions of this important pharmaceutical agent.