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Peoniflorin

Peoniflorin is a monoterpene glycoside found in various Paeonia species, including the common garden peony (Paeonia lactiflora).
It has been the focus of numerous studies due to its potential therapeutic properties, including anti-inflammatory, antioxidant, and neuroprotective effects.
Peoniflorin has been investigated for its use in the treatment of conditions such as rheumatoid arthritis, Alzheimer's disease, and cerebral ischemia.
Researchers can leverage PubCompare.ai's AI-driven platform to optimize their peoniflorin research by quickly locating relevant protocols from the literature, preprints, and patents, and using intelligent comparisons to identify the best protocols and products.
This can enhance the reproducibility and accuracy of peoniflorin studies, empowering researchers to take their research to the next level and uncover new insights about this versatile natural compound.

Most cited protocols related to «Peoniflorin»

1
Identifying Potential Drug Targets
Cited 2 times
Input all the active compounds into SciFinder (http://scifinder.cas.org), a database of chemical and bibliographic information attached to the Chemical Abstracts Service; get the molecular structure of each active compound. Draw them in ChemBioDraw and save as “mol2” file format. Import them into PharmMapper (http://lilab.ecust.edu.cn/pharmmapper/, updated in September 2012), which is a web server for potential drug target identification using pharmacophore mapping approach [28 (link)]. Because of the nonstandard naming, we used UniProtKB (http://www.uniprot.org/), which is the central hub for the collection of functional information on proteins, with accurate, consistent, and rich annotation. Input the protein names with the species limited to “Homo sapiens” and we could receive their official symbol. After these operations, protein information of active compounds was obtained. Finally, we utilized Kyoto Encyclopedia of Genes and Genomes (KEGG) database (http://www.genome.jp/kegg/, updated in May 2016) for noting pathway and filtering out protein targets that can be noted by human signal pathway. We used saikosaponin a and saikosaponin d instead of saikosaponin and used peoniflorin instead of TGP to obtain targets because of their high activity. The details are described in Table S2.
Liu H., Zeng L., Yang K, & Zhang G. (2016). A Network Pharmacology Approach to Explore the Pharmacological Mechanism of Xiaoyao Powder on Anovulatory Infertility. Evidence-based Complementary and Alternative Medicine : eCAM, 2016, 2960372.
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Publication 2016
Drug Delivery Systems Genome Homo sapiens Molecular Structure peoniflorin Proteins saikosaponin D Signal Pathways
2
Standardized SNS Herbal Decoction Preparation
Cited 1 time
SNS consists of Chaihu (Radix Bupleuri Chinensis, derived from Bupleurum chinense DC, voucher number 16012002), Baishao (Radix Paeoniae Alba, derived from Paeonia lactiflora Pall, voucher number 16040201), Zhishi (Fructus Aurantii Immaturus, derived from Citrus aurantium L, voucher number 16011370), and Gancao (Radix Glycyrrhizae, derived from Glycyrrhiza uralensis Fisch, voucher number 15091001) with a ratio of 1:1:1:1.
These raw herbs were purchased from Beijing Xinglin Pharmaceutical Company and were identified as eligible medicinal material. SNS was prepared by the Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University. Specifically, a total weight of 400 g of the above raw herbs was mixed and impregnated in 2400 ml distilled water for 30 min. Then the medical materials were boiled for 30 min and 400 ml SNS preparation was harvested. This procedure was repeated, and a final volume of 800 ml was obtained from each 400 g herbs. The decoction was stored at 4 °C until the experiment. The major components of SNS decoction were saikosaponins, peoniflorin, naringin and glycyrrhizic acid [10 (link)–12 (link)].
Chang X., Zhao L., Wang J., Lu X, & Zhang S. (2017). Sini-san improves duodenal tight junction integrity in a rat model of functional dyspepsia. BMC Complementary and Alternative Medicine, 17, 432.
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Publication 2017
Bupleurum chinense Bupleurum root Citrus aurantium fructus aurantii immaturus glycyrrhizae radix et rhizoma Glycyrrhiza uralensis Glycyrrhizic Acid naringin Paeonia peoniflorin Pharmaceutical Preparations Plant Roots saikosaponin D
3
Evaluating Tight Junction Function in RNECs
In addition to determining the integrity of the monolayers, cell TEER was measured to investigate changes in TJ function in RNECs. After the cells formed monolayers, 0.5 mL of drug solution was added to the A side, and 1.5 mL of HBSS was added to the B side to simulate A→B transport. B→A transport was similarly evaluated by adding 1.5 mL of drug solution to the B side and 0.5 mL of HBSS to the A side. The TEER values of the untreated cells and of cells treated with puerarin, peoniflorin, and (or) menthol were determined at 30, 60, 90, 120, 150, and 180 minutes. The measured TEER value before the experiment was set as 100%, and all other values were calculated relative to this value. Then, the relative TEER value at each time point was compared with the control group value and statistically analyzed.
Zhang L., Du S.Y., Lu Y., Liu C., Wu H.C., Tian Z.H., Wang M, & Yang C. (2017). Puerarin transport across rat nasal epithelial cells and the influence of compatibility with peoniflorin and menthol. Drug Design, Development and Therapy, 11, 2581-2593.
Publication 2017
Cells Hemoglobin, Sickle Menthol peoniflorin Pharmaceutical Solutions puerarin
4
Evaluating Barrier Function of Herb Compounds
Puerarin (PubChem compound identifier [CID]: 5281807), peoniflorin (PubChem CID: 442534), and menthol (PubChem CID: 16666) were obtained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Polyethylene terephthalate (PET) cell culture inserts and 12-well plates (12-mm diameter, 0.4-μm pore size) were purchased from Corning Corporation (Corning, NY, USA). The rabbit anti-zonula occludens 1 (ZO-1) antibody (61-7300) and the mouse anti-claudin-1 antibody (2H10D10) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Tetramethyl rhodamine isothiocyanate (TRITC, red)-conjugated anti-rabbit IgG antibody was purchased from Beijing Zhongshan Golden Bridge Biotechnology Co, Ltd (Beijing, China). Fluorescein isothiocyanate (FITC, green)-conjugated anti-mouse IgG antibody was purchased from Kangwei Century Biotechnology Co, Ltd (Beijing, China). Acti-stain 488 (green) fluorescent phalloidin was purchased from Cytoskeleton Inc (Denver, CO, USA). 2-(6-(7-nitrobenz-2-oxa-,3-diazol-4-yl)amino) hexanoyl-1-hexadecanoyl-sn-glycero-3-phosphocholine (NBD-C6-HPC) was purchased from Thermo Fisher Scientific. A total protein bicinchoninic acid assay kit, a Na+-K+-ATPase assay kit, and a Ca2+-ATPase assay kit were purchased from NanJing JianCheng Bioengineering Institute (NanJing, China). In this study, all experimental protocols were approved by the Review Committee for the Use of Human or Animal Subjects of Beijing University of Chinese Medicine.
Zhang L., Du S.Y., Lu Y., Liu C., Wu H.C., Tian Z.H., Wang M, & Yang C. (2017). Puerarin transport across rat nasal epithelial cells and the influence of compatibility with peoniflorin and menthol. Drug Design, Development and Therapy, 11, 2581-2593.
Publication 2017
Animals anti-IgG Antibodies, Anti-Idiotypic bicinchoninic acid Biological Assay Biological Products Ca(2+)-Transporting ATPase Cell Culture Techniques Chinese Claudin-1 Cytoskeleton Fluorescein Fluorescein-5-isothiocyanate Glycerylphosphorylcholine Homo sapiens Immunoglobulin G Immunoglobulins isothiocyanate Menthol Mus Na(+)-K(+)-Exchanging ATPase peoniflorin Phalloidine Pharmaceutical Preparations Polyethylene Terephthalates Proteins puerarin Rabbits Stains tetramethylrhodamine isothiocyanate Tight Junctions
5
Quantifying Cytotoxicity of Phytochemicals
The levels of puerarin, peoniflorin, and menthol that interfered with the growth of RNECs were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye assay. The cells were seeded at a density of 1×105 cells/mL in 96-well flat-bottomed microtiter plates in a total volume of 100 μL of culture medium per well and incubated in a humidified atmosphere with 5% CO2 at 37°C. After 48 hours, the medium was removed and replaced with fresh medium containing different compounds at various concentrations. After further culture for 24 hours, 20 μL of 5 mg/mL MTT in PBS was added to each well, and the mixtures were incubated at 37°C for 4 hours until purple deposits became visible. The assay measured the amount of MTT reaction products produced by mitochondrial dehydrogenase and assumed that cell viability, corresponding to the reductive activity, was proportional to the production of purple formazan, which was measured spectrophotometrically. After the MTT solutions were discarded, the colored reaction products were completely dissolved by the addition of 20 μL of dimethyl sulfoxide, and the absorbance was measured at 490 nm on a Multiskan GO microplate reader (Thermo Fisher Scientific). The mean absorbance of five measurements for each compound was expressed as a percentage of the absorbance of the untreated control and plotted against the concentration of the compound. The cells were used at passage numbers 2–3.
Zhang L., Du S.Y., Lu Y., Liu C., Wu H.C., Tian Z.H., Wang M, & Yang C. (2017). Puerarin transport across rat nasal epithelial cells and the influence of compatibility with peoniflorin and menthol. Drug Design, Development and Therapy, 11, 2581-2593.
Publication 2017
Atmosphere Biological Assay Bromides Cells Cell Survival Formazans Menthol Mitochondrial Inheritance Oxidoreductase peoniflorin puerarin Sulfoxide, Dimethyl

Most recents protocols related to «Peoniflorin»

1
HPLC Analysis of YNFJ Herbal Extract

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
2
HPLC Analysis of BCD Granule Stability
BCD granule stability and quality were evaluated utilizing a high-performance liquid chromatography (HPLC) system (Agilent 1100, USA) equipped with a Waters Xbridge-C18 analytical column (4.6 × 150 mm, 5 μm). Mobile phase A indicated the acetonitrile organic phase, while mobile phase B indicated the ultrapure water (containing 0.1% phosphoric acid) aqueous phase. Mobile phases were altered based on the following: 0–5 min, 10% (phase A); 5–35 min, 10%–20% (phase A); 35–50 min, 20%–30% (phase A); 50–55 min, 30%–37% (phase A), 55–60 min, 37%–10% (phase A). The flow rate was 1.0 ml/min and the UV spectrum was calibrated to 230 nm. Specnuezhenide (CAS: 39011-92-2), cyasterone (CAS: 17086-76-9), ferulic acid (CAS: 537-98-4) and isorhamnetin-3-O-neohesperidoside (CAS: 55033-90-4) were procured from National Institutes for Food and Drug Control (Beijing, China), and hyperoside (CAS: 482-36-0), asperosaponin VI (CAS: 39524-08-8), peoniflorin (CAS: 23180-57-6) and typhaneoside (CAS: 104472-68-6) were obtained from Shanghai Standard Technology Co., Ltd. (Shanghai, China). The detection wavelength of BCD was identified based on retention time compared with reference substance.
Chen Y., Fan X., Ma K., Wang K., Tian C., Li M, & Gong L. (2022). Bushen Culuan Decoction Ameliorates Premature Ovarian Insufficiency by Acting on the Nrf2/ARE Signaling Pathway to Alleviate Oxidative Stress. Frontiers in Pharmacology, 13, 857932.
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Publication 2022
acetonitrile akebia saponin D cyasterone Cytoplasmic Granules ferulic acid Food High-Performance Liquid Chromatographies hyperoside isorhamnetin-3-O-neohesperidoside peoniflorin phosphoric acid Retention (Psychology) specnuezhenide typhaneoside
3
Extraction and Quantification of SGFD
Not available on PMC !
To prepare of the SGFD extract, 225 g Baishao, 225 g Zhigancao and 75 g Fuzi were mixed together and then macerated in 10-fold volume of double-distilled water for 30 min, and after boiling with high heat, keep slight boiling with gentle fire for 1 h followed by filtering with absorbent cotton. The decoction step was repeated two times. The filtrates were combined and concentrated under reduced pressure. The powder of SGFD (125 g) was obtained by freeze-drying machine and stored at -20 for further use. The assay of contents was calculated by the external standard method according to the LC-MS/MS method established by our laboratory. The average contents of the main components in SGFD were 29.06 mg/g for peoniflorin, 9.16 mg/g for liquiritin, 0.31 mg/g for benzoylmesaconine, 0.0029 mg/g for mesaconitine, and 16.99 mg/g for glycyrrhizic acid, respectively.
Lin L., Wang Y., Shao S., Lin W., Huang D., Pan R., & Xia Y. (2020). Effects of Shaoyao-Gancao-Fuzi Decoction on the Pharmacokinetics of CYP3A4-Mediated Tofacitinib in Rats.
Publication 2020
4
Network Analysis of IPF-Related Genes Regulated by DLP and DHP
Networks analysis of the targeted IPF related genes regulated by DLP and DHP were performed as described in our previous study (Lyu et al., 2017 (link), 2018 (link)). The main source of disease targets for IPF was obtained from IPA1 database. According to our previous study (Dong J. et al., 2013), 15 compounds used as marker substances of DLP were uploaded into the IPA system to enable the discovery visualization, including gallic acid, puerarin, daidzin, peoniflorin, naringin, rosmarinic acid, salvianolic acid A, formononetin, calycosin, ethyl gallate, lipoteichoic acid, chlorogenic acid, ferulic acid, cryptotanshinone and tanshinone IIA. Similarly, 14 compound were chosen as major ingredients of DHP for IPA analysis, such as L-proline, L-phenylalanine, caffeic acid, danshensu, rutin, hydroxysafflor yellow A, safflor yellow A, salvianolic acid B, uridine, syringin, chlorogenic acid, ferulic acid, rosmarinic acid, salvianolic acid A (Liu et al., 2013 (link)). “Build-Path Explorer” module was applied to discover IPF-related targets, and the relationship between the targets and DLP or DHP ingredients, respectively.
Shao R., Wang F.J., Lyu M., Yang J., Zhang P, & Zhu Y. (2019). Ability to Suppress TGF-β-Activated Myofibroblast Differentiation Distinguishes the Anti-pulmonary Fibrosis Efficacy of Two Danshen-Containing Chinese Herbal Medicine Prescriptions. Frontiers in Pharmacology, 10, 412.
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Publication 2019
3,4-dihydroxyphenyllactic acid 7,3'-dihydroxy-4'-methoxyisoflavone caffeic acid Candidate Gene Analysis Chlorogenic Acid cryptotanshinone daidzin ethyl gallate ferulic acid formononetin Gallic Acid hydroxysafflor yellow A lipoteichoic acid naringin peoniflorin Phenylalanine Proline puerarin rosmarinic acid Rutin salvianolic acid A salvianolic acid B syringin tanshinone II A Uridine
5
Validated UHPLC-MS/MS Method for Rat Plasma Analysis
The UHPLC-MS/MS method developed for determining the 10 components in rat plasma (Figure 1)—namely genipin-1-β-D-gentiobiodide, scandoside methyl ester, geniposide, geniposidic acid, chlorogenic acid, capillarisin, rhein, emodin, caffeic acid, and 4-hydroxyacetophenone—was validated in terms of specificity, accuracy, precision, linearity, lower limit of quantitation (LLOQ), stability, recovery, and matrix effect, in accordance with United States Food and Drug Administration guidelines. Calibration curves were constructed using the peak area ratios of the analytes to peoniflorin and by applying a weighted (1/x2) least squares linear regression analysis. The LLOQ was determined at the lowest concentrations at which the signal-to-noise (S/N) ratio was 10. Specificity was determined by comparing chromatograms of blank rat plasma obtained from five individual subjects with chromatograms of plasma samples obtained after YCHD administration at a dose of 12 g/kg. Precision [expressed as the relative standard deviation (RSD)] and accuracy [expressed as the relative error (RE)] were calculated for three QC points (low, medium, and high). Five replicates of each QC point were analyzed to determine the interday accuracy and precision. This process was repeated three times over three consecutive days to determine the intraday accuracy and precision. Recovery was evaluated in five replicates at three different QC concentrations (low, medium, and high). The percentage recovery was determined by comparing the concentrations of the pre-extraction spiked QC samples prepared in blank matrix (by adding analytes and peoniflorin to blank matrix prior to extraction) with the peak area of the post-extraction spiked QC samples prepared in an extracted blank matrix (prepared by adding analytes and peoniflorin to blank matrix extract). Matrix effects were investigated on five independent sources of blank rat plasma by calculating the ratio of the peak area in the presence of matrix to the peak area in absence of matrix at three different QC concentrations (low, medium, and high).
The stability of standard analytes in rat plasma was evaluated under various conditions (time and temperature) by analyzing five replicates of the QC samples at three concentrations (low, medium, and high). Stability was investigated in terms of short-term and long-term stability, freeze and thaw stability, and post-preparative stability by using the developed method. Short-term stability was evaluated by storing QC samples at room temperature (25°C) for 24 h. Long-term stability was assessed after 60 days of storage at -20°C. Freeze and thaw stability was determined after three freeze–thaw cycles at -20°C. In addition, post-preparative stability during storage in an auto sampler at 4°C for 24 h was investigated.
Yi Y.X., Ding Y., Zhang Y., Ma N.H., Shi F., Kang P., Cai Z.Z, & Zhang T. (2018). Yinchenhao Decoction Ameliorates Alpha-Naphthylisothiocyanate Induced Intrahepatic Cholestasis in Rats by Regulating Phase II Metabolic Enzymes and Transporters. Frontiers in Pharmacology, 9, 510.
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Publication 2018
4-hydroxyacetophenone caffeic acid capillarisin Chlorogenic Acid Emodin Esters Freezing genipin geniposide geniposidic acid peoniflorin Plasma rhein Tandem Mass Spectrometry

Top products related to «Peoniflorin»

1
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1,1-diphenyl-2-picrylhydrazyl (DPPH) is a stable free radical compound commonly used as a reagent in chemical analysis. It is a dark-colored crystalline solid with the molecular formula C18H12N5O6. DPPH is primarily utilized in antioxidant activity assays to measure the free radical scavenging capacity of various substances.
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Acetonitrile by Merck Group
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Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
3
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HPLC-grade methanol is a high-purity solvent for use in high-performance liquid chromatography (HPLC) applications. It is a clear, colorless liquid with a characteristic odor. The product is tested and certified to meet the strict purity requirements for HPLC analysis.
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More about "Peoniflorin"

Peoniflorin, a monoterpene glycoside found in various Paeonia species, has garnered significant attention due to its remarkable therapeutic potential.
This natural compound is present in the common garden peony (Paeonia lactiflora) and has been the subject of extensive research, unveiling its anti-inflammatory, antioxidant, and neuroprotective properties.
Researchers have investigated the use of peoniflorin in the treatment of several conditions, including rheumatoid arthritis, Alzheimer's disease, and cerebral ischemia.
To optimize peoniflorin research, scientists can leverage the AI-driven platform of PubCompare.ai, which allows them to quickly locate relevant protocols from the literature, preprints, and patents.
By utilizing intelligent comparisons, researchers can identify the best protocols and products, enhancing the reproducibility and accuracy of their peoniflorin studies.
This empowers researchers to take their investigations to new heights and uncover novel insights about this versatile natural compound.
Furthermore, researchers can leverage various analytical techniques to study peoniflorin, such as the use of 1,1-diphenyl-2-picrylhydrazyl (DPPH) for antioxidant assays, acetonitrile and HPLC-grade methanol for chromatographic analysis, and an academic ultrapure water system and XBridge C18 analytical column for high-performance liquid chromatography (HPLC) applications.
Harnessing the power of AI-driven insights and advanced analytical methods, researchers can unlock the full potential of peoniflorin and contribute to the growing body of knowledge surrounding this remarkable natural compound.