> Chemicals & Drugs > Organic Chemical > Percoll

Percoll

Percoll is a colloidal silica-based media used for cell separation and purification.
It is commonly employed in density gradient centrifugation to isolate specific cell types, organelles, and other biological particles.
Percoll optimization is crucial for achieving high-purity and reproducibile results in a variety of research applications, including immunology, neuroscience, and stem cell biology.
PubCompare.ai is an AI-driven platform that helps researchers identify the most effective Percoll protocols and products by leveraging advanced comparison tools across scientific literature, pre-prints, and patents.
This cutting-edge platform can streamline your workflow and enable you to achieve reliable, reproducible results for your Percoll-based experiments.

Most cited protocols related to «Percoll»

Artemisinin Resistance Evaluation Protocol

Cited 62 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2013

artenimol Atmosphere Biological Assay BLOOD Cell Nucleus Erythrocytes Gentamicin Heparin Sodium Parasitemia Parasites Percoll Pharmaceutical Preparations Schizonts Sorbitol Sulfoxide, Dimethyl Thermal Plasma Trophozoite Volumes, Packed Erythrocyte

Optimized Isolation of Mouse Hepatocytes

Cited 59 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2020

Berries BLOOD Calcium Cannulation Cell Cycle Checkpoints Cells ChIP-Chip Collagenase Dental Anesthesia Digestion Extracellular Matrix Friend Hepatocyte Liberase Liver Mice, House Percoll Perfusion Veins, Portal Venae Cavae

In vitro Culture and Genetic Manipulation of P. falciparum

Cited 36 times

P. falciparum parasites were grown in vitro in RPMI 1640 medium containing 0.5% w/v AlbumaxII as described^{42 (link)}. Parasites were synchronised by purifying schizont stages using a 70% Percoll gradient, before allowing reinvasion to occur, and followed by sorbitol treatment. All parasites used in this study were derived from the 3D7 clone, including the control 1G5DC strain^{13 (link)}. Plasmids were introduced into purified schizont stage parasites by electroporation using an Amaxa 4D-Nucleofector™ (Lonza) as previously described^{43 (link)}. For transfections with reporter plasmids to be maintained episomally 10 μg of DNA was electroporated and cultures were selected continuously with 2.5 μg/ml of blasticidin-S-HCl. For generation of marker-free, DiCre transgenic parasites 20–30 μg of CRISPR/Cas9 plasmids and 60 μg of linearised rescue plasmids were electroporated. Selection was applied one day after transfection with 5 nM WR99210 (a kind gift of Jacobus Pharmaceuticals) for four days. Following establishment of transgenic parasites, the cultures were treated with 1 μM 5-fluorocytosine (5-FC) provided as clinical grade Ancotil^® (MEDA) for one week before parasite cloning by limiting dilution. To induce DiCre recombinase mediated excision of DNA, early ring stage parasites were treated with rapamycin (stock solution was 200 μM in DMSO), before being washed in warm RPMI 1640 medium and returned to culture.

Knuepfer E., Napiorkowska M., van Ooij C, & Holder A.A. (2017). Generating conditional gene knockouts in Plasmodium – a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR/Cas9. Scientific Reports, 7, 3881.

Full text: Click here

Publication 2017

Ancobon Animals, Transgenic blasticidin S Clustered Regularly Interspaced Short Palindromic Repeats Electroporation Parasites Percoll Pharmaceutical Preparations Plasmids Recombinase Schizonts Sirolimus Sorbitol Sulfoxide, Dimethyl Technique, Dilution Transfection

Mitochondrial Proteome Profiling of Mouse Tissues

Cited 34 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2008

Amino Acids Antibodies Bistris Calreticulin Digestion Genes Ions Mice, House Mice, Inbred C57BL Mitochondria Organelles Peptides Percoll Proteins Protein Subunits Radionuclide Imaging SDS-PAGE Staphylococcal Protein A Tandem Mass Spectrometry Tissues Trypsin VDAC1 protein, human

In Vitro Cultivation and Genetic Manipulation of Malaria Parasite P. falciparum

Cited 33 times

Asexual blood-stage cultures of P. falciparum clone 3D7 were maintained in vitro and synchronized according to standard procedures (Blackman, 1994 (link); Yeoh et al., 2007 (link)) in RPMI 1640 medium containing the serum substitute Albumax (Invitrogen). For introduction of transfection constructs, mature schizonts were enriched from highly synchronous cultures using Percoll (GE Healthcare) as described previously (Harris et al., 2005 (link)), and transfected by electroporation with 10 μg of circular plasmid DNA using the Amaxa 4D electroporator (Lonza) and the P3 Primary cell 4D Nucleofector X Kit L (Lonza) and program FP158, exactly as recently described for P. knowlesi (Moon et al., 2013 (link)). Selection for parasites harbouring the plasmid was performed by culture in medium containing 2.5 nM WR99210. Selection for parasites in which integration of transfected DNA into the genome had taken place was promoted by cycles of culture in the absence and presence of WR99210, as described previously (Harris et al., 2005 (link)). When integration was detected by diagnostic PCR, integrant clones were obtained by limiting dilution and maintained in medium containing WR99210. Parasite growth rates were assessed by microscopic examination of Giemsa-stained thin films at 2-day intervals, and expressed as percentage parasitaemia (percentage of parasitized erythrocytes in the population)

Collins C.R., Das S., Wong E.H., Andenmatten N., Stallmach R., Hackett F., Herman J.P., Müller S., Meissner M, & Blackman M.J. (2013). Robust inducible Cre recombinase activity in the human malaria parasite Plasmodium falciparum enables efficient gene deletion within a single asexual erythrocytic growth cycle. Molecular Microbiology, 88(4), 687-701.

Full text: Click here

Publication 2013

BLOOD Clone Cells Diagnosis DNA, Circular Electroporation Erythrocytes Genome L Cells Microscopy Parasitemia Parasites Percoll Plasmids Schizonts Serum Stain, Giemsa Technique, Dilution Transfection

Most recents protocols related to «Percoll»

Mutagenesis and Lipid Production

Not available on PMC !

Example 6

Strain 5 was subjected to another round of mutagenesis with increasing concentrations and exposure time to 4-NQO (37 μM for 30 minutes at 28° C.). This population of cells was subsequently subdivided and grown in standard lipid production medium supplemented with a range of cerulenin concentrations (7-50 μM). Cells from all concentrations were pooled and fractionated over a 60% Percoll/0.15 M NaCl density gradient. Oil laden cells recovered from a density zone of 1.02 g/mL were plated and assessed for glucose consumption and fatty acid profile. One of these clones was subsequently stabilized and given the strain designation “Strain 6”.

US11873405B2. High oleic oil compositions and uses thereof (2024-01-16). Checkerspot, Inc. [US]. Inventors: Leon Parker [US], Kevin Ward [US], Scott Franklin [US], Mona Correa [US], Veronica Benites [US].

Full text: Click here

Patent 2024

Cells Cerulenin Clone Cells Fatty Acids Glucose Lipids Microalgae Mutagenesis Oleic Acid Percoll Sodium Chloride Strains Triglycerides

Strain 4 Mutagenesis and Enrichment

Not available on PMC !

Example 4

As an alternative to stabilizing Strain 3, a new round of mutagenesis was pursued for Strain 2 utilizing treatment of cells with the mutagen 4-nitroquinoline-1-oxide (4-NQO) for 5 minutes at 28° C. Mutagenized cells were enriched by growing under conditions of limited glucose (14 g/L) for three days, then the cells were subjected to fraction over a 60% Percoll/0.15 M NaCl density gradient. Cells recovered from a density zone of 1.06 g/mL were plated and assessed for glucose consumption and fatty acid profile. One of these clones was subsequently stabilized and given the strain designation “Strain 4”.

Full text: Click here

Patent 2024

Cells Clone Cells Cultured Cells Fatty Acids Glucose Microalgae Mutagenesis Mutagens Oleic Acid Oxides Percoll Sodium Chloride Strains Triglycerides

Quantitative ER-Mitochondria Interaction Assay

Mouse monoclonal anti ACTIN, Sigma-Aldrich, A5441; Mouse monoclonal anti c-myc, Sigma-Aldrich, M4439; Rabbit polyclonal anti Calnexin, Enzo, ADI-SPA-865-F; Mouse monoclonal anti CYTC, BD Bioscience, 556433; Rabbit DyLight 680, Thermo Fisher Scientific, 35569; Mouse DyLight 680 Thermo Fisher Scientific, 35519; Mouse DyLight 800 Thermo Fisher Scientific, 35521; Rabbit DyLight 800 Thermo Fisher Scientific, 35571; Mouse monoclonal anti eIF2α, Cell Signaling, 2103S; Rabbit polyclonal anti-E-Syt1, Sigma-Aldrich, HPA016858; Rabbit polyclonal anti-GFP, Cell Signaling, 2555S; Mouse monoclonal anti-GFP,Life technologies, A11122; HRP Mouse Bioké, Cell Signaling, 7076; HRP Rabbit Bioké, Cell Signaling, 7074; Mouse monoclonal anti IP3R3, BD Bioscience, 610312; Rabbit polyclonal anti-PERK, Cell signaling, 3192S; Rabbit polyclonal anti PERK, Cell signaling, 5683S; Rabbit monoclonal anti Phospho-eIF2α (Ser51), Cell signaling, 3597S; Rabbit polyclonal anti PDI Genetex, GTX30716; Mouse monoclonal anti PSD, Santa Cruz, sc-390070; Rabbit polyclonal anti, PSS1 (B-5), Santa Cruz, sc-515376; Rabbit polyclonal anti PSS2, Sigma-Aldrich, SAB1303408; Rabbit polyclonal VDAC1, Cell Signaling, 4866S; Rabbit polyclonal VDAC1, Abcam, ab15895; Veriblot antibody Abcam, ab131366.
The reagents used were: Antimycin A, Sigma-Aldrich, A8674; Calcium Chloride dihydrate, Sigma-Aldrich, C3881; CHAPS hydrate, Sigma-Aldrich, C3023; Conjugated GFP antibody beads, Laboratory of Chris Ulens; D-Galactose, Sigma-Aldrich, G0750; D-glucose, Sigma-Aldrich, G7021-1KG; DAPI, Thermo Fisher Scientific, 62248; Dulbecco’s Modified Eagle’s Medium - high glucose, Sigma-Aldrich, D0422; EGTA, AppliChem, A0878; FCCP, Sigma-Aldrich, C2920; Gibco DMEM/F-12, Thermo Fisher Scientific, 11320074; Glucose, Agilent Seahorse, 103577; Glutamine, Sigma-Aldrich, G7513; Glutamine, Agilent Seahorse, 103579; GSK PERK Inhibitor, Toronto Research Company, G797800; Hygromycin B, Invivogen, ant-hg-1; Lipofectamine 2000 Transfection Reagent, Thermo Fisher Scientific, 11668019; MitoTracker FarRed, Thermo Fisher Scientific, M22426; NBD-PS, Avanti Polar Lipids, 810194C; SE Cell Line 4D-Nucleofector X Kit L, V4XC-1024; Oligomycin, Sigma-Aldrich, 75351; Penicillin and streptomycin, Sigma-Aldrich, P0781; Percoll, Sigma-Aldrich, P1644; Pierce ECL Western Blotting Substrate, Thermo Fisher Scientific, 32106X4; Pierce Protein A/G Magnetic Beads, Thermo Fisher Scientific, 88802; Pierce Protease Inhibitor Tablets, EDTA-free, Thermo Fisher Scientific, 88266; Potassium Chloride, Janssen Chimica, 7447407; Protease inhibitor, Thermo Fisher Scientific, A32953; Puromycin, Thermo Fisher Scientific, A11138-03; Protein A/G PLUS-Agarose, Santa Cruz, sc-2003; XF DMEM pH7 7.4, Agilent Seahorse, 103575; Sodium Chloride, Sigma-Aldrich, A0431796; Sodium Pyruvate Solution, Agilent Seahorse, 103578; Sucrose, Acros, A0333146; Thapsigargin, Enzo Life Sciences, BML-PE180; TransIT-X2 Dynamic Delivery System, Mirus Bio, MIR 6000; Tris base, Sigma-Aldrich, 77861; Triton, Sigma-Aldrich, T9234; Tween, Sigma Aldrich, P4780.

Sassano M.L., van Vliet A.R., Vervoort E., Van Eygen S., Van den Haute C., Pavie B., Roels J., Swinnen J.V., Spinazzi M., Moens L., Casteels K., Meyts I., Pinton P., Marchi S., Rochin L., Giordano F., Felipe-Abrio B, & Agostinis P. (2023). PERK recruits E-Syt1 at ER–mitochondria contacts for mitochondrial lipid transport and respiration. The Journal of Cell Biology, 222(3), e202206008.

Full text: Click here

Publication 2023

3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate Actins Antimycin A Calcium Chloride Dihydrate Calnexin Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone DAPI Eagle Edetic Acid Egtazic Acid G-substrate Galactose Glucose Glutamine Hygromycin B Immunoglobulins L Cells Lipids lipofectamine 2000 Mus N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylserine Obstetric Delivery Oligomycins Peeling Skin Syndrome Peeling skin syndrome, acral type Penicillins Percoll Potassium Chloride Protease Inhibitors Puromycin Pyruvate Rabbits Seahorses Sepharose Sodium Sodium Chloride Staphylococcal Protein A Streptomycin Sucrose SYT1 protein, human Thapsigargin Transfection Tromethamine Tweens VDAC1 protein, human

Mitochondria and MAMs Isolation

Cells were collected and the resulting pellet after centrifugation (600–800 g for 5 min at RT) was resuspended in 5 ml of Starting Buffer 1 (SB1) containing 225 mM mannitol, 75 mM sucrose and 30 mM Tris–HCl, 0.1 mM EGTA, pH 7.4 and homogenized. Unbroken cells and nuclei were removed by centrifugation of the cell homogenate at 600 g for 5 min (at 4°C). The crude mitochondrial fraction (mito crude) was pelleted by centrifuging the supernatant at 7,000 g for 10 min at 4°C while the supernatant contained the ER. The crude mitochondria were resuspended in SB (225 mM mannitol, 75 mM sucrose and 30 mM Tris–HCl, pH 7.4) and after other two sequential centrifugations (7,000 g and 10,000 g for 10 min at 4°C), the obtained pellet was resuspended in 1 ml of mitochondria resuspending buffer MRB (250 mM mannitol, 5 mM HEPES and 0.5 mM EGTA), layered on top of a percoll gradient (30 and 15%) and spun down at 95,000 g for 40 min (using a Beckman ultracentrifuge, rotor SW41), to separate MAMs from the pure mitochondria (mito pure). Further ultracentrifugation (70Ti rotor, 100,000 g for 1 h at 4°C) was required to obtain the pellet of the MAMs fraction.

Full text: Click here

Publication 2023

Buffers Cell Nucleus Cells Centrifugation Egtazic Acid G-800 HEPES Mammography Mannitol Mitochondria Mitochondrial Inheritance Mitomycin Percoll Sucrose Tromethamine Ultracentrifugation

Isolation and Culture of Neonatal Rat Cardiomyocytes

NRCMs were prepared as previously described^{54 (link)}. NRCMs were obtained from 0- to 2-day-old Wistar rats (Takasugi Experimental Animal Supply, Saitama, Japan), dispersed by collagenase digestion, and subjected to Percoll gradient centrifugation. Isolated cardiomyocytes were cultured for 48 h in Dulbecco’s modified Eagle medium (Nacalai Tesque, Kyoto, Japan) supplemented with 10% fetal calf serum. After 24 h of serum starvation, NRCMs were stimulated with SA and PE.

Bujo S., Toko H., Ito K., Koyama S., Ishizuka M., Umei M., Yanagisawa-Murakami H., Guo J., Zhai B., Zhao C., Kishikawa R., Takeda N., Tsushima K., Ikeda Y., Takimoto E., Morita H., Harada M, & Komuro I. (2023). Low-carbohydrate diets containing plant-derived fat but not animal-derived fat ameliorate heart failure. Scientific Reports, 13, 3987.

Full text: Click here

Publication 2023

Animals, Laboratory Centrifugation Collagenase Digestion Eagle Fetal Bovine Serum Myocytes, Cardiac Percoll Rats, Wistar Serum

Top products related to «Percoll»

Percoll by GE Healthcare

Sourced in United States, Sweden, United Kingdom, Germany, Canada, Japan, Denmark, Switzerland, Italy, Australia, Spain, Norway, Belgium, Macao

Percoll is a colloidal silica-based medium used for cell separation and gradient centrifugation. It is designed to provide a density gradient for the isolation and purification of cells, organelles, and other biological particles.

Percoll by Merck Group

Sourced in United States, Germany, United Kingdom, Canada, Italy, Sao Tome and Principe, Australia, Belgium, France, Macao, Norway, Ireland

Percoll is a colloidal silica-based density gradient medium used for the separation and purification of cells, organelles, and other biological particles. It is designed to create density gradients for the isolation of specific cell types or subcellular fractions through centrifugation.

Dnase 1 by Merck Group

Sourced in United States, Germany, Switzerland, United Kingdom, Italy, Japan, Macao, Canada, Sao Tome and Principe, China, France, Australia, Spain, Belgium, Netherlands, Israel, Sweden, India

DNase I is a laboratory enzyme that functions to degrade DNA molecules. It catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, effectively breaking down DNA strands.

Dnase 1 by Roche

Sourced in United States, Switzerland, Germany, Japan, United Kingdom, France, Canada, Italy, Macao, China, Australia, Belgium, Israel, Sweden, Spain, Austria

DNase I is a lab equipment product that serves as an enzyme used for cleaving DNA molecules. It functions by catalyzing the hydrolytic cleavage of phosphodiester bonds in the DNA backbone, effectively breaking down DNA strands.

Collagenase d by Roche

Sourced in Switzerland, United States, Germany, United Kingdom, France, Japan, Canada, Australia, Ireland

Collagenase D is an enzyme solution used for the dissociation and isolation of cells from various tissues. It is a mixture of proteolytic enzymes that cleave the collagen present in the extracellular matrix, allowing for the release of individual cells.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Percoll gradient by GE Healthcare

Sourced in United States, Sweden, United Kingdom, Germany

Percoll gradient is a colloidal silica-based medium used for the separation and isolation of cells, organelles, and other biological particles through density gradient centrifugation. It provides a gentle, non-toxic environment that preserves the integrity and function of the separated components.

Collagenase 4 by Merck Group

Sourced in United States, Germany, United Kingdom, France, China, Sao Tome and Principe, Israel, Canada, Macao, Italy, Australia, Japan, Switzerland, Senegal

Collagenase IV is a purified enzyme used to dissociate and isolate cells from various tissue types. It is effective in breaking down collagen, a major structural component of the extracellular matrix.

Percoll by Cytiva

Sourced in United States, Sweden, United Kingdom, Japan

Percoll is a colloidal silica-based medium designed for the separation of cells and subcellular particles by density gradient centrifugation. It is a sterile, pyrogen-free, and non-toxic solution that can be used for the isolation and purification of various cell types and organelles.

Percoll gradient by Merck Group

Sourced in United States, Germany, United Kingdom, China

Percoll gradient is a colloidal silica-based density gradient media used for the separation and purification of cells, organelles, and other biological particles. It provides a gentle, non-toxic environment for the isolation of sensitive biological materials. Percoll gradient enables the separation of different cell types or sub-cellular components based on their distinct buoyant densities.

What are the common applications of Percoll in research?

Percoll is widely used in a variety of research fields, including immunology, neuroscience, and stem cell biology. It is commonly employed in density gradient centrifugation to isolate specific cell types, organelles, and other biological particles with high purity and reproducibility. Percoll optimization is crucial for achieving reliable results in these diverse research applications.

What are the key factors to consider when optimizing Percoll protocols?

When optimizing Percoll protocols, researchers should consider factors such as cell type, sample composition, and desired purity levels. The density and viscosity of the Percoll solution, as well as the centrifugation speed and duration, can significantly impact the separation efficiency and final product quality. Proper optimization is essential to ensure high-purity and reproducible results.

How can PubCompare.ai help researchers identify the most effective Percoll protocols?

PubCompare.ai is an AI-driven platform that can streamline the process of finding the most effective Percoll protocols. By leveraging advanced comparison tools across scientific literature, pre-prints, and patents, the platform can help researchers screen protocol information more efficiently and identify the key differences in protocol effectiveness. This enables researchers to choose the best Percoll protocol for their specific research goals, promoting reproducibility and accuracy in their experiments.

What are the different types or variations of Percoll available for cell separation?

Percoll is available in various formulations and densities to suit different cell separation needs. For example, Percoll can be used to isolate lymphocytes, monocytes, granulocytes, and other cell types by adjusting the density gradient. Additionally, some researchers may use pre-made Percoll solutions or create customized gradients to achieve optimal separation for their specific samples and research requirements.

How can PubCompare.ai help researchers overcome common challenges with Percoll-based cell separation?

PubCompare.ai's AI-driven analysis can help researchers overcome common challenges associated with Percoll-based cell separation, such as poor reproducibility or low purity. The platform's comparison tools can highlight the critical differences between Percoll protocols, enabling researchers to identify the most effective approaches for their research needs. By leveraging this insigth, researchers can make informed decisions and optimize their Percoll-based workflows to achieve reliable and reproducible results.

More about "Percoll"

Percoll, a silica-based media, is a crucial tool in cell separation and purification.
It is commonly used in density gradient centrifugation to isolate specific cell types, organelles, and other biological particles.
Optimizing Percoll protocols is key for achieving high-purity and reproducible results in a variety of research applications, including immunology, neuroscience, and stem cell biology.
Percoll gradients can be used in conjunction with other reagents like DNase I, Collagenase D, and FBS to enhance cell isolation and purification.
Collagenase IV, for example, is often used to dissociate tissues and release cells prior to Percoll-based separation.
PubCompare.ai is an innovative AI-driven platform that helps researchers identify the most effective Percoll protocols and products.
By leveraging advanced comparison tools across scientific literature, pre-prints, and patents, this cutting-edge platform can streamline your workflow and enable you to achieve reliable, reproducible results for your Percoll-based experiments.
Unlock the power of Percoll optimization with PubCompare.ai and take your research to the next level.