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Phloroglucinol

Phloroglucinol is a natural polyphenolic compound found in various plants.
It displays a wide range of biological activities, including antioxidant, anti-inflammatory, and antimicrobial properties.
Phloroglucinol has been studied for its potential therapeutic applications in areas such as skin health, wound healing, and neuroprotection.
Researchers use a variety of protocols to isolate, analyze, and evaluate the efficacy of phloroglucinol in their studies.
PubCompare.ai's AI-driven platform can help optimize these research protocols by easily locating and comparing methods from literature, pre-prints, and patents.
This intelligent system assists researchers in navigating the vast research landscape and making informed decisions for their phloroglucinol studies, experincing the future of protocol optimization today.

Most cited protocols related to «Phloroglucinol»

Cranberry fruit (Vaccinium macrocarpon Ait.) was collected at the Marucci Center for Blueberry and Cranberry Research, Chatsworth, NJ. Purified C-PAC extract was isolated from cranberries of the ‘Early Black’ cultivar utilizing solid-phase chromatography according to well established methodology [9 (link)–12 (link)]. In brief, the fruit was homogenized in 70% aqueous acetone, filtered and the pulp discarded. Collected cranberry-derived proanthocyanidins were concentrated under reduced pressure and purified extract isolated using bioassay-directed fractionation. The absence of absorption at 360 nm and 450 nm confirm all but proanthocyanidins are removed. Additional methods including 13C NMR, electrospray mass spectrometry, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and acid catalyzed degradation with phloroglucinol were utilized to verify the presence of A-type linkages as well as to determine the concentration of proanthocyanidins in the purified extract. C-PAC is comprised of five main proanthocyanidins as previously characterized by Dr. Howell and colleagues [11 (link)]. The proanthocyanidin molecules largely consist of epicatechin units with degrees of polymerization of 4 or 5, as well as epigallocatechin and catechin. C-PAC contains three types of linkages, two common B-type linkages (C4→C6 and C4→C8) and at least one unique A-type ether linkage (C2→O→C7) found only in cranberry, chokeberry, plums and avocado [14 (link), 15 (link)]. Purified C-PAC was freeze-dried and stored at −80°C. C-PAC concentrations chosen for study were informed by our earlier research which determined the LD50 to be in the 50 to 100 μg/ml range in various cancer cell lines [16 (link)–18 (link)]. Consideration was also given to earlier evaluations by Howell and colleagues showing 50 μg/ml of C-PAC inhibits adhesion of p-fimbriated uropathogenic E. coli bacteria in vitro and that 36 mg/day of C-PAC delivered in 10 ounces of juice inhibits bacterial adhesion in the urinary tract wall of humans [9 (link)–13 (link)]. Importantly, the concentrations of C-PAC under evaluation in this series of preclinical investigations are readily achievable in humans and are already under evaluation for oral and urinary tract health benefits.
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Publication 2015
Acetone Acids Bacteria Bacterial Adhesion Biological Assay Blueberries Carbon-13 Magnetic Resonance Spectroscopy Cardiac Arrest Catechin Cell Lines Chromatography Cranberry Dental Pulp Epicatechin epigallocatechin Ethyl Ether Fractionation, Chemical Freezing Fruit Homo sapiens Malignant Neoplasms Mass Spectrometry Persea americana Phloroglucinol Plum Polymerization Pressure proanthocyanidin Proanthocyanidins Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Urinary Tract Uropathogenic Escherichia coli Vaccinium macrocarpon
Hand-cut cross-sections were made from the base of the stem of both the inoculated and mock-treated cotton plants at 14 d after treatment. Lignin histochemistry was examined using Wiesner reagent (Pomar et al., 2004 ). Cross-sections were incubated for 10 min in a phloroglucinol solution (2% in 95% ethanol) or 95% ethanol (staining control), treated with 18% HCl for 5 min, and directly observed with a bright-field microscope using a Leica fluorescence microscope (DM2500, Leica, Wetzlar, Germany).
Publication 2011
Ethanol Gossypium Histocytochemistry Lignin Microscopy Microscopy, Fluorescence Phloroglucinol Stem, Plant
Fucoidan with an average Mw of 735 kDa was provided by MMBI KSC RAS, Murmansk, Russia. Fucoidan was extracted from F. vesiculosus by ultrasound-assisted extraction [72 (link)] according to the procedure described previously [18 (link)]. The yield of fucoidan was 4.9% by mass. Fucoidan contained 79.5% of neutral carbohydrates, 27.0% of sulfate residues, and 0.7% of uronic acid. Carbohydrates were represented by fucose (73.5 mol%), glucose (11.8 mol%), galactose (3.7 mol%), xylose (6.6 mol%), mannose (0.2 mol%), and arabinose (0.2 mol%). The molar ratio of fucose, glucose, galactose, xylose, mannose, and arabinose was 1.0:0.16:0.05:0.09:0.03:0.03, respectively, as evidenced by high performance liquid chromatography (HPLC) [18 (link)]. According to the literature data, fucoidans from Fucus spp. are represented mainly by (1→3)- and (1→4)-linked α-l-fucopyranose residues [3 (link),36 (link)]. Electrospray ionization mass spectrometry evidenced that sulfate groups are attached mostly at C-2 and sometimes at C-4 of sugar residues in fucoidans from brown seaweeds [73 (link)].
The concentration of total polyphenols was determined with Folin–Ciocalteu reagent as described previously [74 (link)]. Phloroglucinol (Sigma, St. Louis, MO, USA) was used as reference. The total polyphenol content was 4.7 ± 0.5 mg PGEq g−1 (milligram of Phloroglucinol equivalent PGEq per 1 g fucoidan).
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Publication 2020
Arabinose Carbohydrates folin fucoidan Fucose Fucus Galactose Glucose High-Performance Liquid Chromatographies Mannose Molar PER1 protein, human Phloroglucinol Polyphenols Seaweed Spectrometry, Mass, Electrospray Ionization Sugars Sulfates, Inorganic Ultrasonics Uronic Acids Xylose
Plants were grown for 3 weeks under short-day conditions (8 h light, 16 h dark), and then shifted to long-day conditions (16 h light, 8 h dark) to induce flowering. JA treatments were performed by watering plants with either tap water (mock) or 0.5 mm jasmonic acid after moving plants to long days. Due to the asymmetric effects of side shoots on tissue patterning, only plants in which the first internode was at least 3.5 cm long were analysed. For histological analyses, stem fragments were fixed in FAA (formalin/acetic acid/alcohol) and embedded in paraffin. Subsequently, 10 μm sections were produced using a microtome, deparaffinized, stained with 0.05% toluidine blue (AppliChem, http://www.applichem.com), and fixed with Entellan (Merck, http://www.merck.com) or Dako Ultramount (Dako, http://www.dako.com) (Figure 6) on microscope slides. For quantitative analyses, at least five plants were evaluated for each data point. The standard errors of means were used to visualize variation. Data were subjected to statistical analysis, using a two-tailed independent Student’s t test with SPSS 15.0 software (http://www.spss.com). Significance levels of P<0.05, P<0.01 and P<0.001 are indicated in the figures by single, double and triple asterisks, respectively. Phloroglucinol staining and analysis of GUS reporter activity were performed as described previously (Ruzin, 1999 ; Scarpella et al., 2004 (link)). For analysis of signal distribution in cross-sections (Figure 7), stained samples were left in 30% sucrose overnight at 4°C, then embedded in 5% low-melting-point agarose (Sigma, http://www.sigmaaldrich.com/) and sectioned using a vibratome (HM430, Microm, http://www.microm-online.com). The resulting 30 μm sections were observed using DIC optics. Alternatively, samples were embedded in Technovit 7100 (Kulzer, http://www.kulzer.com) using the manufacturer’s protocol, and 5 μm sections were produced with a microtome, fixed with Entellan and observed using dark-field optics (Figure S3).
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Publication 2010
Acetic Acid entellan Ethanol Eye Formalin jasmonic acid Light Microscopy Microtomy Paraffin Embedding Phloroglucinol Plants Sepharose Stem, Plant Student Sucrose Technovit 7100 Tissues Tolonium Chloride
P90, E90, E80, and E70 chromatographic profiles were obtained with an HPLC system (Jasco, Tokyo, Japan) equipped with a PU‐980 pump, an UV‐1575 detector, and a degasser Populaire DP4003. Data were obtained and processed with PowerChrom 2.5 (eDaq Technologies, NZ, Australia) software and MATLAB R2019b software (MathWorks Inc., USA). Chromatographic separation was performed on a Kromasil C‐18 semi‐preparative column (8 × 250 mm) at 30°C. A gradient elution using water (A) and methanol (B) consisted of 0–3 min (99% A, 1% B), 30 min (5% A, 95% B), and 40 min (99% A, 1% B). Freeze‐dried samples (2 mg/ml) were diluted in EtOH 20% (v/v) and filtered through 0.45 µm syringe filters. Injection volume was 50 µl, detection wavelength 266 nm, and solvent flow rate 0.4 ml/min. Polyphenols content was calculated with standard molecules of phloroglucinol and resorcinol; additionally, glucose and glucuronic acid standards were injected to check for possible interference of UA and sugars (Gonçalves‐Fernández et al., 2019 (link)). To ensure the reproducibility of the assays, a minimum of four injections of each extract were carried out.
Publication 2022
Biological Assay Chromatography Ethanol Freezing Glucose Glucuronic Acid High-Performance Liquid Chromatographies Methanol Phloroglucinol Polyphenols resorcinol Solvents Sugars Syringes

Most recents protocols related to «Phloroglucinol»

The assessment of lignin deposition on vascular tissue (xylem) was performed using the method described by Saxena et al. [26 ]. The control and pre-treated chilli plants with bioagents were used for the observation of lignin deposition on cell-wall. After 30 and 60 days of treatment with bioagents, the second internodal region of the stem was selected. The transverse section of the stem of treated and untreated samples was made with hand-cut approx. 0.5 mm thickness with the help of a steel blade. The sections were fixed in 1% (w/v) phloroglucinol solution containing 50% HCl in the ratio of 3:1 and kept for 5 min. After that, the sections were mounted with glycerin on a glass side and covered with a coverslip. The stained tissue was observed under Olympus binocular microscope. The deposition of lignin in stem tissues was examined as red-pink color. The experiment was repeated thrice.
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Publication 2023
Blood Vessel Cell Wall Glycerin Lignin Microscopy Phloroglucinol Plants Steel Stem, Plant Tissues Tissue Stains Xylem

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Publication 2023
Darkness folin Phloroglucinol

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Publication 2023
Fast Blue BB Phloroglucinol Radionuclide Imaging

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Publication 2023
Acetate Ethanol Phloroglucinol Radionuclide Imaging

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Publication 2023
Acids Biological Assay Chlorides fast blue 2B salt folin Gallic Acid Phenol Phloroglucinol Radionuclide Imaging Vision zinc chloride

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Phloroglucinol is a chemical compound that is commonly used as a reagent in various analytical and laboratory applications. It is a colorless, crystalline solid that is soluble in water and organic solvents. Phloroglucinol serves as a precursor for the synthesis of other organic compounds and is often used as a detection agent in chemical analysis.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Ascorbic acid is a chemical compound commonly known as Vitamin C. It is a water-soluble vitamin that plays a role in various physiological processes. As a laboratory product, ascorbic acid is used as a reducing agent, antioxidant, and pH regulator in various applications.
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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
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The Folin-Ciocalteu reagent is a colorimetric reagent used for the quantitative determination of phenolic compounds. It is a mixture of phosphomolybdic and phosphotungstic acid complexes that undergo a color change when reduced by phenolic compounds.
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Catechin is a natural polyphenolic compound found in various plants, including green tea. It functions as an antioxidant, with the ability to scavenge free radicals and protect cells from oxidative stress.
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Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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Epicatechin is a natural compound found in various plants and is commonly used in laboratory settings. It serves as a standard reference material for analytical and research purposes. Epicatechin exhibits antioxidant properties and is often employed in the evaluation of antioxidant activity and the development of analytical methods.
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Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.

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Polyphenols, natural compounds, antioxidants, anti-inflammatory, antimicrobial, skin care, wound healing, neuroprotection, research protocols, literature review, pre-prints, patents, AI-driven platform, protocol optimization, research landscape, informed decisions