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Phosphomolybdic acid

Phosphomolybdic acid is an inorganic compound with the chemical formula H3PMo12O40.
It is a yellow, crystalline solid that is soluble in water and ethanl.
Phosphomolybdic acid is commonly used as an oxidizing agent, a reagent in analytical chemistry, and a catalyst in organic synthesis.
It has applications in the detection and quantification of various biomolecules, such as proteins, carbohydrates, and alkaloids.
Phosphomolybdic acid can also be used to stain biological specimens for microscopic examination.
Researchers can utilize PubCompare.ai's AI-driven optimiztion to find the most reproducible protocols for working with phosphomolybdic acid, exploring pre-prints, patents, and literaure to identify the best products and procedures for their experiments.

Most cited protocols related to «Phosphomolybdic acid»

1
Contrast Staining Protocols for Microscopy
Cited 63 times
The most broadly useful contrast stains tested so far are inorganic iodine and phosphotungstic acid (PTA)[22 (link)]. The formulations and general procedures used are given in Table 2, and notes on the fixatives used are in Table 3[23 -25 (link)]. The stains and procedures are simple and the procedures are robust. The staining times were found not to be critical, as long as the stain had sufficient time to penetrate the tissues. Inorganic iodine in alcoholic or aqueous solution diffuses rapidly into fixed tissues and was able to stain most specimens in a few hours or less, although staining was generally done overnight. PTA is a much larger molecule [26 (link)], and the solution used here was found to require overnight incubation to penetrate specimens 2–3 mm thick, and longer for larger specimens. PTA is known to bind heavily to various proteins and connective tissue [27 ,28 ], and this property, along with electron-shell energies that match common x-ray source emissions, suggested that it might be a useful stain for x-ray imaging. A few samples were tested with phosphomolybdic acid (PMA) staining, used similarly to PTA. The results (not shown) were generally similar, and PMA was not pursued further here (but see refs. [29 (link)] and [30 ] for successful application of PMA).
, & Metscher B.D. (2009). MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues. BMC Physiology, 9, 11.
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Publication 2009
Alcoholics Connective Tissue Electrons Fixatives Iodine phosphomolybdic acid Phosphotungstic Acid Proteins Radiography Stains Tissues
2
Quantitative Synovial Tissue Immunohistochemistry
Cited 8 times
All enrolled patients underwent ultrasound-guided synovial tissue biopsy of the knee following the already published protocol.8 (link) Once collected, synovial tissue specimens were fixed in 10% neutral-buffered formalin and embedded in paraffin for histology. Briefly, paraffin-embedded synovial tissue (ST) specimens were sectioned at 3–4 μm. First, sections were stained for H&E as follows: sections were deparaffinised in xylene and rehydrated in a graded ethanol series. Then they were stained in haematoxylin and counterstained in eosin/phloxine. Finally, sections were dehydrated, cleared in xylene and mounted with Bio Mount (Bio-Optica). Other sections were stained for CD68 mouse antihuman monoclonal antibody (clone 514H12) or CD21 mouse antihuman monoclonal antibody (clone 2G9) or CD20 mouse antihuman monoclonal antibody (clone L26) or CD3 mouse antihuman monoclonal antibody (clone LN10) or CD31 mouse antihuman monoclonal antibody (clone 1A10) (all from Leica Biosystem, Newcastle, UK) by immunostainer BOND MAX III (Leica).9
ST slides were stained for collagen using the Masson Trichrome Goldner with light green (Bio-Optica; 04-011802) as follows: slides were washed in distilled water; then six drops of Weigert's iron haematoxylin, provided by the manufacturer, were put on the sections and left to act 10 min. Without washing, the slides were drained, and 10 drops of picric acid alcoholic stable solution were added on the sections. Then, the slides were quickly (3–4 s) washed in distilled water, and 10 drops of ponceau acid fuchsin according to Masson were added on the sections and left to act 4 min. Then, the slides were washed in distilled water, and 10 drops of phosphomolybdic acid solution were added on the sections and left to act 10 min. Without washing, the slides were drained, and 10 drops of Light Green Solution, according to Goldner, were added and left to act 5 min. Then, the slides were washed in distilled water and rapidly dehydrated through ascending alcohols. Reaction was done for 1 min in absolute alcohol. Finally, the slides were cleared in xylene and mounted.
Slides were examined by two independent evaluators using a light microscope (Leica DM 2000), and all tissues were evaluated using a numerical score based on the number of CD68+, CD21+, CD3+, CD20+ and CD31+ cells in the lining and sublining areas of the section (three different fields in each section), with a score of 0 indicating no positive cells; 1 indicating <10% positive cells; 2 indicating 10%–50% positive cells; and 3 indicating >50% positive cells.10 (link) The inter-rater agreement coefficient was assessed for each single immunohistochemistry (IHC) marker (see online supplementary table S1).
Alivernini S., Tolusso B., Petricca L., Bui L., Di Sante G., Peluso G., Benvenuto R., Fedele A.L., Federico F., Ferraccioli G, & Gremese E. (2017). Synovial features of patients with rheumatoid arthritis and psoriatic arthritis in clinical and ultrasound remission differ under anti-TNF therapy: a clue to interpret different chances of relapse after clinical remission?. Annals of the Rheumatic Diseases, 76(7), 1228-1236.
Publication 2017
Absolute Alcohol acid-fuchsin Alcoholics Biopsy Cells Clone Cells Collagen Eosin Ethanol Formalin Hematoxylin Immunohistochemistry Iron Knee Light Microscopy Methyl Green Monoclonal Antibodies Mus ocaratuzumab Paraffin Paraffin Embedding Patients phloxine phosphomolybdic acid picric acid Synovial Membrane Tissues Ultrasonography Xylene
3
Antioxidant Capacity Assessment of Dioscorea palmatum
Cited 7 times
Frozen leaves (2 g) were homogenized with 10 mL of 0.05 M sodium phosphate buffer solution (pH 7.8) with 1% polyvivylpyrrolidone and was centrifuged at 12,000× g at 4 °C for 15 min. The supernatant was collected for malondialdehyde (MDA) and enzyme analysis. MDA content was measured using spectrophotometric assay based on the thiobarbituric acid reaction [101 (link)]. Superoxide dismutase activity was analyzed using spectrophotometric method of Giannopolitis-Ries with measurements of the inhibition levels of nitroblue tetrazolium reduction at 560 nm [102 (link)]. Catalase activity was measured using spectrophotometric method of Abassi et al. which include the measuring of H2O2 extinction decline at 240 nm [103 (link)].
The total antioxidant capacity of D. palmatum extracts was determined using the phosphomolybdic acid method [104 (link)]; the DPPH• radical scavenging activity and ABTS•+ radical scavenging activity was assessed as described early [105 (link)]; the determination of superoxide anion scavenging activity (O2•−-SA) was measured in phenazine methosulfate-nicotinamide adenine dinucleotide-nitroblue tetrazolium systems using the method of Ozen et al. [106 (link)]; the Br• radical scavenging activity (Br•-SA) was determined using culometric method [105 (link)] with electrogenerated bromine radicals; the potential to inactivate NO was assessed by the sodium nitropusside technique [107 (link)]; the ability to inactivate H2O2 was determined using the technique developed by Badami and Channabasavaraj [108 (link)]; to evaluate the chelating activity for Fe2+ ions the o-phenanthroline technique was applied [59 (link)].
For preparation of the extract, accurately-weighed dried sample of D. palmatum herb (10 g) was transferred in a conical flask for preparation of the extracts. After that, 150 mL of solvent (60% methanol solutions) was added with stirring and put in an ultrasonic bath. The extraction conditions were 60 min at 45 °C, ultrasound power of 100 W, the frequency 35 kHz. The extraction was repeated twice. The obtained extract were filtered through a cellulose filter and combined. The filtrates were evaporated in vacuo until dryness with the use of a rotary evaporator.
Olennikov D.N., Chirikova N.K., Kashchenko N.I., Gornostai T.G., Selyutina I.Y, & Zilfikarov I.N. (2017). Effect of Low Temperature Cultivation on the Phytochemical Profile and Bioactivity of Arctic Plants: A Case of Dracocephalum palmatum. International Journal of Molecular Sciences, 18(12), 2579.
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Publication 2017
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid Antioxidants Bath Biological Assay Bromine Buffers Catalase Cellulose Coenzyme I Enzymes Extinction, Psychological Freezing Ions Malondialdehyde Methanol Methylphenazonium Methosulfate Nitroblue Tetrazolium o-phenanthroline Peroxide, Hydrogen phosphomolybdic acid Psychological Inhibition Sodium sodium phosphate Solvents Spectrophotometry Superoxide Dismutase Superoxides thiobarbituric acid Ultrasonics
4
Immunostaining and Trichrome Staining of Cell and Tissue Samples
Cited 6 times
Cells in lrECM gel were smeared on slides, dried briefly, and fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100. Immunostaining was performed as previous described [32 (link)]. Stained samples were imaged with a Nikon upright epifluorescence microscope or a confocal system comprised of an Olympus IX81 microscope.
Xenograft tumor sections were de-paraffined and hydrated from xylene, 100% ethanol, 95% ethanol, 85% ethanol and 70% ethanol to distilled water. For Masson’s trichrome staining, slides were re-fixed with Bouin’s solution at 60°C for 60 minutes. Slides were washed in running tap water for 5 minutes and stained in Weigert’s working hematoxyin for 10 minutes. Then they were washed in running tap water for 5 minutes and stained in Biebrich scarlet-acid fuchsin solution for 5 minutes. Slides were rinsed in distilled water and differentiated in phosphomolybdic-phosphotungstic acid solution for 10 minutes, transferred to aniline blue solution and stain for 5 minutes. Slides were rinsed in distilled water and images were taken with a Nikon microscope. The percentage of collagen was quantified by calculating the ratio of blue staining (collagen) area in the total area of the tumor section using Imagescope analysis software [33 (link)].
Xiong G., Deng L., Zhu J., Rychahou P.G, & Xu R. (2014). Prolyl-4-hydroxylase α subunit 2 promotes breast cancer progression and metastasis by regulating collagen deposition. BMC Cancer, 14, 1.
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Publication 2014
aniline blue Biebrich Scarlet Cells Collagen Ethanol Microscopy Neoplasms paraform Phosphotungstic Acid Stains Triton X-100 Xenografting Xylene
5
Histological Analysis of Kidney Fibrosis
Cited 5 times
All the kidney tissues were excised, fixed in 4% formaldehyde and embedded in paraffin. Kidney tissue sections (5 μm) were obtained and used for histological and immunohistochemical analyses. H&E staining was performed according to the standard H&E protocol. The injury score was acquired by modified Banff classification criteria in ten randomly selected non-overlapping fields per rat H&E stained kidney tissues59 (link). All specimens were blindly evaluated by one nephrologist.
For renal fibrosis, Masson’s trichrome staining from the each group was used to evaluate the extent of renal fibrosis according to the standard Masson’s trichrome protocol. Briefly, kidney tissue sections were successively immersed into Weigert’s iron hematoxylin, Biebrich scarlet-acid fuchsin, phosphomolybdic-phosphotungstic acid, and aniline blue. To quantify the renal fibrosis, the blue pixel contents of the images were photographed with the same microscope and magnification times. Ten different views in each group were selected to detect the values of the integral optical density and the total area and the expression intensity was calculated as the percentage of the integral optical density to the total area which was performed by Image-Pro Plus 6.0 (Media Cybernetics, Inc.).
Zhang Z.H., Wei F., Vaziri N.D., Cheng X.L., Bai X., Lin R.C, & Zhao Y.Y. (2015). Metabolomics insights into chronic kidney disease and modulatory effect of rhubarb against tubulointerstitial fibrosis. Scientific Reports, 5, 14472.
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Publication 2015
acid-fuchsin aniline blue Biebrich Scarlet Fibrosis Formaldehyde Hematoxylin Injuries Iron Kidney Microscopy Nephrologists Paraffin Embedding phosphomolybdic acid Tissues

Most recents protocols related to «Phosphomolybdic acid»

1
Phytochemical Extraction Procedure
Not available on PMC !
Chloroform, methanol, ethanol, n-hexane, acetone, n-butanol, acetic acid, phosphomolybdic acid, sodium tungstate, sodium carbonate, phosphoric acid, gallic acid, β-carotene, Tween 40, and butylated hydroxyanisole (BHA) were procured from the Fisher Scientific Co. (Nepean, ON, Canada) and Sigma-Aldrich Ltd. (Oakville, ON, Canada).
Onodenalore A.C., Hossain A., Banoub J., & Shahidi F. (2024). Unique heterocyclic phenolic compounds from shrimp (Pandalus borealis) and beyond. Food Production Processing and Nutrition, 6(1).
Publication 2024
2
Masson's Trichrome Staining of Colon Tissue
The colon tissues
were cut into sections (each 5-μm thick) and then stained using
Masson’s trichrome staining following the established procedure.
After a short period of deparaffinization, the sections were submerged
in Bouin solution and left to incubate at 37 °C overnight. The
sections underwent a 2–3 min staining process using Celestine
blue after being rinsed with distilled water. Subsequently, the samples
were washed with water and colored using Mayer hematoxylin solution
for a duration of 2–3 min. After that, the sections underwent
another water wash and were differentiated with an acidic ethanol
solution for a brief period. Following a 10 min rinse with running
water, the sections were stained using Li Chunhong Acid Fuchsin Staining
solution, and 1% phosphomolybdic acid was used to differentiate the
sections for 10 min. After extraction from phosphomolybdic acid, the
samples underwent staining using aniline blue solution, cleansing
with distilled water, and a 2 min treatment with a mild acid solution.
Eventually, the slices underwent dehydration using 95% and 100% alcohol,
followed by sealing.
Ji K., Zhang M., Du L., Wang J., Liu Y., Xu C., He N., Wang Q., Gu Y., Song H., Wang Y, & Liu Q. (2024). Exploring the Role of Inulin in Targeting the Gut Microbiota: An Innovative Strategy for Alleviating Colonic Fibrosis Induced By Irradiation. Journal of Agricultural and Food Chemistry, 72(11), 5710-5724.
Publication 2024
3
Lowry Protein Quantification Assay
Not available on PMC !
Serum protein concentration was determined by the method of Lowry. 16 Alkaline Copper sulfate solution catalyzes the oxidation of aromatic amino acids followed by the reduction of phosphomolybdic and phosphotungstic acid, resulting in a purple color complex whose intensity is proportional to the quantity of aromatic amino acid, which is measured at 660 nm.
Pandian N.S., Sankar H.R., Ramalingam S., & Saravanan R. (2024). Hepatoprotective Effect of Hydroalcoholic Extract of Vitis vinifera L Seeds on Paracetamol-Induced Liver Damage in Wistar Rats. Tropical Journal of Natural Product Research, 8(2).
Publication 2024
4
Picrosirius Red Staining Protocol
Picrosirius red (PSR) staining was conducted by Van Andel Institute Pathology and Biorepository Core using a standard protocol (Dolber and Spach, 1987 (link)). Briefly, tissue sections 5-μm-thick tissue sections were deparaffinized, treated with phosphomolybdic acid before staining with Sirius red. Slides were washed in hydrochloric acid and 70% EtOH before cover slipping for polarized light imaging.
Arumugam M., Tovar E.A., Essenburg C.J., Dischinger P.S., Beddows I., Wolfrum E., Madaj Z.B., Turner L., Feenstra K., Gallik K.L., Cohen L., Nichols M., Sheridan R.T., Esquibel C.R., Mouneimne G., Graveel C.R, & Steensma M.R. (2024). Nf1 deficiency modulates the stromal environment in the pretumorigenic rat mammary gland. Frontiers in Cell and Developmental Biology, 12, 1375441.
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Publication 2024
5
Microwave-Assisted Masson's Trichrome Staining
Microwave procedure for Masson’s trichrome kit (HT15-1KT, Sigma-Aldrich, Germany) was used for infarct size estimation with the omission of counter staining with hematoxylin. Skin was the positive control.
5μm slides were prepared from paraffin blocks and mounted on Poly L-lysine coated slides. These slides were dewaxed in xylene and hydrated with decreasing gradations of alcohol and placed in a Coplin jar filled with Bouin’s solution (500ml distilled water was saturated with picric acid and then filtered 167ml formaldehyde and 33.3ml glacial acetic acid were then added to it). This was microwaved for 15seconds followed by a 5mins incubation in fume hood. Slides were then washed with tap water until yellow color on the slides disappeared. Slides were then stained with Biebrich Scarlet-Acid Fuchsin and microwaved for 12seconds, followed by a two minute incubation and then placed in Phosphotungstic/Phosphomolybdic acid (10ml Phosphotungstic acid and 10ml Phosphomolybdic acid is mixed in 20ml deionized water) and microwaved for 12seconds followed by 5mins incubation. Slides were then placed in Analine Blue solution, microwaved for nine seconds, followed by a one minute incubation. After several washes of deionized water, slides were placed in 1% acetic acid (1N acetic acid) for 45 seconds and washed with deionized water for two mins. This was followed by sequential dehydration with alcohol and clearing with two changes of xylene. Slides were then mounted with DPX.
Zuberi S., Rafi H., Hussain A, & Hashmi S. (2024). Upregulation of Nrf2 in myocardial infarction and ischemia-reperfusion injury of the heart. PLOS ONE, 19(3), e0299503.
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Publication 2024

Top products related to «Phosphomolybdic acid»

1
Phosphomolybdic acid by Merck Group
Sourced in United States, Germany
Phosphomolybdic acid is a chemical compound with the formula H3[P(Mo3O10)4]. It is a yellow, crystalline solid that is commonly used as a reagent in analytical chemistry.
2
Bouin s solution by Merck Group
Sourced in United States, Germany, Japan, France, United Kingdom
Bouin's solution is a laboratory fixative used for the preservation and staining of biological samples. It contains a mixture of picric acid, formaldehyde, and acetic acid. Bouin's solution is commonly used in histological and cytological procedures to prepare tissue samples for microscopic examination.
3
Kieselgel 60 by Merck Group
Sourced in Germany, United States, Italy, Sweden, Japan
Kieselgel 60 is a silica gel used as a stationary phase in column chromatography. It is a highly porous, amorphous form of silicon dioxide with a high surface area. Kieselgel 60 is commonly used for the separation and purification of organic compounds.
4
Silica gel 60 f254 by Merck Group
Sourced in Germany, United States, India, Japan, Switzerland
Silica gel 60 F254 is a type of silica gel thin-layer chromatography (TLC) plate. It is a planar solid support material used for the separation and identification of chemical compounds. The silica gel 60 F254 plate contains a fluorescent indicator that allows for the visualization of separated compounds under ultraviolet (UV) light.
5
Silica gel 60 by Merck Group
Sourced in Germany, United States, Japan, Italy, Switzerland, India, Egypt, Sweden, Canada, China, United Kingdom, Brazil
Silica gel 60 is a porous, amorphous form of silicon dioxide commonly used as a stationary phase in column chromatography. It has a high surface area and is effective at adsorbing a wide range of organic and inorganic compounds. Silica gel 60 is available in various particle sizes and pore sizes to suit different chromatographic applications.
6
Aniline blue by Merck Group
Sourced in United States, Germany
Aniline blue is a synthetic dye used as a staining agent in various laboratory applications. It is a deep blue or purple colored powder that is soluble in water. Aniline blue is commonly used to stain nucleic acids, carbohydrates, and other biological structures for visualization and analysis purposes.
7
Permount by Thermo Fisher Scientific
Sourced in United States, Canada, Japan, Germany, United Kingdom, Gabon, China
Permount is a mounting medium used in microscopy to permanently mount specimens on glass slides. It is a solvent-based, xylene-containing solution that dries to form a clear, resinous film, securing the specimen in place and providing optical clarity for microscopic examination.
8
Aniline blue solution by Merck Group
Sourced in United States, Germany
Aniline blue solution is a laboratory reagent used for staining and visualization of biological samples. It contains the dye aniline blue, which binds to and stains polysaccharides and other carbohydrate-containing structures. This solution is commonly used in various microscopy and histological applications.
9
Image pro plus 6 by Media Cybernetics
Sourced in United States, Japan, Germany, United Kingdom, China, Hungary, Singapore, Canada, Switzerland
Image-Pro Plus 6.0 is a comprehensive image analysis software package designed for scientific and industrial applications. It provides a wide range of tools for image capture, enhancement, measurement, analysis, and reporting.
10
Phosphomolybdic phosphotungstic acid solution by Merck Group
Sourced in Germany, United States
Phosphomolybdic/phosphotungstic acid solution is a reagent used in analytical chemistry. It is a mixture of phosphomolybdic acid and phosphotungstic acid, which are employed as oxidizing agents in various colorimetric and spectrophotometric assays.

More about "Phosphomolybdic acid"

Phosphomolybdic acid (PMA) is a versatile inorganic compound with the chemical formula H3PMo12O40.
It is a yellow, crystalline solid that is soluble in water and ethanol.
PMA has numerous applications in analytical chemistry, organic synthesis, and biological research.
As an oxidizing agent, PMA is commonly used in the detection and quantification of various biomolecules, such as proteins, carbohydrates, and alkaloids.
PMA-based staining techniques are also employed to visualize biological specimens under microscopic examination.
Researchers can utilize PubCompare.ai's AI-driven optimization to find the most reproducible protocols for working with PMA.
By exploring pre-prints, patents, and scientific literature, they can identify the best products and procedures for their experiments, ensuring more efficient and reliable research.
PMA is often used in conjunction with other reagents and materials, such as Bouin's solution (a fixative used in histology), Kieselgel 60 and Silica gel 60 F254 (adsorbents used in chromatography), Aniline blue (a staining agent), Permount (a mounting medium), and Image-Pro Plus 6.0 (an image analysis software).
The combination of these tools and techniques can enhance the effectiveness and reproducibility of PMA-based experiments.
By leveraging the insights gained from the MeSH term description and the Metadescription, researchers can optimize their workflows and access the most reliable information to advance their understanding and utilization of this versatile compound.