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Plumbagin

Plumbagin is a naphthoquinone compound found in several plant species, particularly the Plumbaginaceae family.
It has demonstrated a range of biological activities, including antimicrobial, anti-inflammatory, and antitumor properties.
Plumbagin research has potential applications in drug development, but repoducubility and optimization of experimental protocols can be challneging.
PubCompare.ai's AI-driven platform can help researchers locate relevant protocols from literature, preprints, and patents, and leverage advanced comparisons to identify the most effective methods and products for Plumbagin studies.
This tool can improve the accuracy and reproducibility of Plumbagin research, supporting the development of new therapeutic applications.

Most cited protocols related to «Plumbagin»

1
Schistosomula Viability Assay Protocol
Cited 8 times
Auranofin, gambogic acid (GA), disulfiram, menadione, oltipraz, parthenolide, plumbagin from Plumbago indica, PZQ, thonzonium bromide, sanguinarine chloride hydrate, dimethyl sulphoxide (DMSO), percoll and fetal bovine serum (FBS) were from Sigma-Aldrich. The Ro 15–5458 compound was a kind gift from Dr H. Stohler (Hoffman-La Roche, Basel, Switzerland) and oxamniquine was provided by Pfizer, London. Drugs were dissolved in DMSO to obtain stock solutions at 10 mM and were then diluted into culture medium. CellTiter-Glo (CTG) reagent, used in the schistosomula viability luminescence-based assay, and CellTox green dye, used in the schistosomula staining, were from Promega. BioWhittaker Dulbecco-Modified Eagle’s Medium (DMEM) lacking phenol red and containing 4500 mg/l glucose, 1 mM Hepes pH 6.98–7.30, 2 mM L-glutamine, 1x antibiotic-antimycotic reagent (Life Technologies) and 10% heat inactivated FBS, was used as tissue culture medium for schistosomula. Adult worms were cultured in BioWhittaker DMEM containing 4500 mg/l glucose, 2 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, 0.5 μg/ml amphothericin B and 10% heat inactivated FBS.
Lalli C., Guidi A., Gennari N., Altamura S., Bresciani A, & Ruberti G. (2015). Development and Validation of a Luminescence-based, Medium-Throughput Assay for Drug Screening in Schistosoma mansoni. PLoS Neglected Tropical Diseases, 9(1), e0003484.
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Publication 2015
Adult Antibiotics Auranofin Culture Media Disulfiram Eagle Fetal Bovine Serum gambogic acid Glucose Glutamine Helminths HEPES Luminescent Measurements oltipraz Oxamniquine parthenolide Penicillins Percoll Pharmaceutical Preparations plumbagin Plumbago Promega Ro 15-5458 sanguinarine chloride Streptomycin Sulfoxide, Dimethyl thonzonium bromide Tissues Vitamin K3
2
Cell Viability and Proliferation Assay
Cited 6 times
Cell viability was evaluated using CCK8 (Beyotime, Jiangsu, China). Cells (2 × 103) were seeded in 96-well plates by different treatment, and cultured for different time points. CCK8 solution (10 μl) was added to each well, and the plates were incubated at 37°C for 1 hour. Absorbance was measured at 450 nm on a microplate reader (Bioteck). DMEM containing 10% CCK8 was used as a control. Proliferating cell count was measured using the Cell-Light EdU DNA Cell Proliferation Kit (RIBOBio Co, Guangzhou, China). The cells were seeded in 96-well culture plates and exposed to media with or without plumbagin. 2000 cells/well were treated with 50 μmol/L of EdU for 2 h at 37 °C. After being fixed with 4% paraformaldehyde for 15 min, the cells were baptized with 0.5% Triton X-100 for 30 minutes and rinsed with PBS three times. Thereafter, the cells were exposed to 100 μL/well of 1×Apollo® reaction cocktail for 30 minutes and incubated with 5 μg/mL of Hoechst 33342 to stain the cell nuclei for 30 minutes. Images were captured using a fluorescent microscope (Olympus, Tokyo, Japan). Experiments were repeated at least three times.
Li Y., Zhao C., Yu Z., Chen J., She X., Li P., Liu C., Zhang Y., Feng J., Fu H., Wang B., Kuang L., Li L., Lv G, & Wu M. (2016). Low expression of miR-381 is a favorite prognosis factor and enhances the chemosensitivity of osteosarcoma. Oncotarget, 7(42), 68585-68596.
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Publication 2016
Cell Nucleus Cell Proliferation Cells Cell Survival Cultural Evolution HOE 33342 Light Microscopy paraform plumbagin Stains Triton X-100
3
Measuring Tyrosinase Activity in B16F10 Cells
Cited 5 times
Cellular tyrosinase activity was determined using a previously described method with modification [25 (link),26 (link)]. Cultured B16F10 cells were incubated with α-MSH in the absence or presence of plumbagin, and cells were then washed and lysed with PBS containing 1% sodium deoxycholate and 0.5% Triton X-100. After the protein concentration was determined, 50 μg of protein was incubated with 2.0 mM l-DOPA and 0.1 M PBS (pH 6.8) for 1 h at 37 °C. The oxidation of l-DOPA was measured at 475 nm (OD475) using an absorbance reader. Activity was measured using the following formula: tyrosinase activity (%) = (OD475 of sample/OD475 of control) × 100.
Oh T.I., Yun J.M., Park E.J., Kim Y.S., Lee Y.M, & Lim J.H. (2017). Plumbagin Suppresses α-MSH-Induced Melanogenesis in B16F10 Mouse Melanoma Cells by Inhibiting Tyrosinase Activity. International Journal of Molecular Sciences, 18(2), 320.
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Publication 2017
alpha Melanocyte Stimulating Hormone Cells Cultured Cells Deoxycholic Acid, Monosodium Salt Levodopa Monophenol Monooxygenase plumbagin Proteins Triton X-100
4
Purification of Dihydroorotase Enzymes
Cited 5 times
The construction of StDHOase [61 (link)], KpDHOase [62 (link)], huDHOase [61 (link)], and ScDHOase [61 (link),63 (link)] expression plasmids were reported previously. The recombinant protein was purified using the protocol described previously for SSB-like proteins [64 (link),65 (link),66 (link),67 (link),68 (link)]. Briefly, E. coli BL21(DE3) cells were transformed with the expression vector, and the overexpression of the expression plasmids was induced by incubating with 1 mM isopropyl thiogalactopyranoside. The protein was purified from the soluble supernatant by using Ni2+-affinity chromatography (HiTrap HP; GE Healthcare Bio-Sciences), eluted with buffer A (20 mM Tris–HCl, 250 mM imidazole, and 0.5 M NaCl, pH 7.9), and dialyzed against a dialysis buffer (20 mM Tris–HCl and 0.1 M NaCl, pH 7.9). The protein purity remained at >97% as determined using SDS–PAGE (Mini-PROTEAN Tetra System; Bio-Rad, CA, USA).
Guan H.H., Huang Y.H., Lin E.S., Chen C.J, & Huang C.Y. (2021). Plumbagin, a Natural Product with Potent Anticancer Activities, Binds to and Inhibits Dihydroorotase, a Key Enzyme in Pyrimidine Biosynthesis. International Journal of Molecular Sciences, 22(13), 6861.
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Publication 2021
Buffers Cells Chromatography, Affinity Cloning Vectors Dialysis Escherichia coli imidazole Plasmids Proteins Recombinant Proteins SDS-PAGE Sodium Chloride Tetragonopterus Tromethamine
5
Trypan Blue Cytotoxicity Assay for 4T1 Cells
Cited 4 times
The trypan blue cytotoxicity assay was performed to assess the cell death and study whether PLU can exhibit cytotoxicity on the 4T1 carcinoma cell survival. The 4T1 cells (1 × 104) were incubated with PLU in a 100 μL volume. After 24 h, the anticancer potentiality was estimated [79 ].
Guan H.H., Huang Y.H., Lin E.S., Chen C.J, & Huang C.Y. (2021). Plumbagin, a Natural Product with Potent Anticancer Activities, Binds to and Inhibits Dihydroorotase, a Key Enzyme in Pyrimidine Biosynthesis. International Journal of Molecular Sciences, 22(13), 6861.
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Publication 2021
Biological Assay Carcinoma Cell Death Cells Cell Survival Cytotoxin Trypan Blue

Most recents protocols related to «Plumbagin»

1
Plumbagin Dose-Response Evaluation
Not available on PMC !
Plumbagin was procured from sigma company in Bangalore. Plumbagin 0.01ppm, 0.02ppm, 0.025ppm, 0.035ppm, 0.05ppm, 0.075ppm and 0.1ppm were dissolved in 1 ml of acetone. It has been used orally and topically.
Gnanamani R., Ramanathan B., & G I.B. (2024). Bioassay Study of Azadirachtin And Plumbagin on Pericallia ricini (Lepidoptera: Arctiidae). Current Agriculture Research Journal, 12(1), 231-241.
Publication 2024
2
Quantitative Analysis of Plumbagin in Samples
Not available on PMC !
Accurately weighed 25mg standard Plumbagin was dissolved in 25 ml of methanol to prepare 1mg/ml solution. Preparation of Test samples: Each 1 gm of sample was taken in conical flask along with 10 ml methanol and kept it overnight. Next day all samples are sonicated and centrifuged. Supernatant liquid is collected and stored in test tube for further use. Application of standard and sample: For spot application, Linomat V sample applicator (CAMAG, 4132 Muttenz, Switzerland) was used and samples solution applied to Silica Gel 60 F254 plate as sharp bands. After that this plate kept aside till the spots get dried. After that the mobile phase, Toluene: Formic acid (9.9:0.1) was poured into Twin trough chamber and chamber covered with lid for 45 minutes to allow saturation. Then lid was removed and plate was inserted into the chamber carefully. Hereafter the plate was allowed to develop until the solvent front had travelled at the distance of 70mm above the base of the plate. After this the plate was taken off from the chamber and dried by using blow dryer and then at 266nm wavelength, detection was done by using CAMAG TLC Scanner III & Win cats 4.02 software. This study was done at CRL (Central Research Laboratory), DMIHER ( Datta Meghe Institute of Higher Education & Research), Sawangi (M), Wardha, Maharashtra.
Kamdi P.S., Gupta R., Khobragade P., & Bahatkar G.K. (2024). Physicochemical and Phytochemical analysis of Wild and Cultivated Chitrak (Plumbago zeylanica Linn.). International Journal of Ayurvedic Medicine, 14(4), 1087-1092.
Publication 2024
3
Preparation of Stock Solutions for Bioassays
PLU was purchased from Aladdin Reagent Ltd. in Shanghai, China. NIT, CPR, TMP, and CHL were obtained from Sigma-Aldrich, while MEC was sourced from Shanghai Yuanye Biological Co., Ltd. in Shanghai, China. The stock solutions of PLU and antibiotics were prepared in water (MEC) or dimethyl sulfoxide (DMSO) (NIT, CPR, TMP, CHL, and PLU) due to the varying solubility of each compound. The solutions were then passed through 0.22 μm filters and stored in the dark at -20°C. Before being used in assays, the stocks were thawed and diluted to the desired concentrations.
Bie S., Mo Q., Shi C., Yuan H., Li C., Wu T., Li W, & Yu H. (2024). Interactions of plumbagin with five common antibiotics against Staphylococcus aureus in vitro. PLOS ONE, 19(1), e0297493.
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Publication 2024
4
Silencing p53 Enhances Plumbagin Effects

Protocol full text hidden due to copyright restrictions

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Publication 2024
5
Plumbagin's Cytotoxic Effects on Colon Cancer

Protocol full text hidden due to copyright restrictions

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Publication 2024

Top products related to «Plumbagin»

1
Plumbagin by Merck Group
Sourced in United States, Germany
Plumbagin is a chemical compound used as a laboratory reagent. It is a naphthoquinone with the chemical formula C11H8O3. Plumbagin is commonly used in various analytical and experimental applications within research and scientific settings.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
4
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
5
Prism 5 by GraphPad
Sourced in United States, Germany, United Kingdom, Israel, Canada, Austria, Belgium, Poland, Lao People's Democratic Republic, Japan, China, France, Brazil, New Zealand, Switzerland, Sweden, Australia
GraphPad Prism 5 is a data analysis and graphing software. It provides tools for data organization, statistical analysis, and visual representation of results.
6
Facscalibur by BD
Sourced in United States, Germany, United Kingdom, China, Canada, Japan, Italy, France, Belgium, Switzerland, Singapore, Uruguay, Australia, Spain, Poland, India, Austria, Denmark, Netherlands, Jersey, Finland, Sweden
The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.
7
Pvdf membrane by Merck Group
Sourced in United States, Germany, China, United Kingdom, Morocco, Ireland, France, Italy, Japan, Canada, Spain, Switzerland, New Zealand, India, Hong Kong, Sao Tome and Principe, Sweden, Netherlands, Australia, Belgium, Austria
PVDF membranes are a type of laboratory equipment used for a variety of applications. They are made from polyvinylidene fluoride (PVDF), a durable and chemically resistant material. PVDF membranes are known for their high mechanical strength, thermal stability, and resistance to a wide range of chemicals. They are commonly used in various filtration, separation, and analysis processes in scientific and research settings.
8
Prism 6 by GraphPad
Sourced in United States, United Kingdom, Canada, China, Germany, Japan, Belgium, Israel, Lao People's Democratic Republic, Italy, France, Austria, Sweden, Switzerland, Ireland, Finland
Prism 6 is a data analysis and graphing software developed by GraphPad. It provides tools for curve fitting, statistical analysis, and data visualization.
9
Lsrii analyzer by BD
Sourced in United States
The BD LSRII analyzer is a flow cytometry instrument designed for multi-parameter analysis of cells and particles. It is capable of detecting and measuring the size, granularity, and fluorescence properties of cells or particles passing through a laser beam. The instrument provides high-resolution data acquisition and analysis for a wide range of applications in life science research.
10
Menadione by Merck Group
Sourced in United States, Germany, United Kingdom, France, Austria, Japan, China, Macao, India, Italy, Poland, Czechia, Switzerland, Belgium, Canada, Brazil
Menadione is a synthetic form of vitamin K. It is a colorless crystalline solid that is used as a dietary supplement and in the production of various pharmaceutical and industrial products. Menadione serves as a precursor for the production of vitamin K-dependent proteins, which are involved in blood clotting and other physiological processes.

More about "Plumbagin"

Plumbagin, a naphthoquinone compound, is a natural product found in various plant species, particularly the Plumbaginaceae family.
This bioactive molecule has demonstrated a wide range of therapeutic potential, including antimicrobial, anti-inflammatory, and antitumor properties.
Plumbagin research has garnered significant interest in the scientific community, as it holds promise for drug development and novel therapeutic applications.
Optimizing Plumbagin research protocols can be challenging, as reproducibility and experimental consistency are crucial.
PubCompare.ai's AI-driven platform offers a unique solution to this challenge.
This advanced tool can help researchers locate relevant protocols from the vast trove of scientific literature, preprints, and patents.
By leveraging AI-driven comparisons, researchers can identify the most effective methods and products for their Plumbagin studies, improving the accuracy and reproducibility of their findings.
The versatility of Plumbagin research extends beyond just its therapeutic potential.
Researchers may also utilize related compounds and techniques, such as FBS (Fetal Bovine Serum) for cell culture, DMSO (Dimethyl Sulfoxide) as a solvent, Penicillin/Streptomycin for antimicrobial protection, and GraphPad Prism 5 or Prism 6 for data analysis.
Flow cytometry, using instruments like the FACSCalibur or BD LSRII analyzer, can provide valuable insights into the cellular effects of Plumbagin.
Additionally, techniques like Western blotting, with PVDF membranes, can elucidate the molecular mechanisms underlying Plumbagin's biological activities.
Plumbagin research has the potential to uncover new therapeutic avenues, but the optimization of experimental protocols is crucial.
PubCompare.ai's AI-driven platform can be a valuable tool in this endeavor, helping researchers navigate the vast scientific landscape and identify the most effective methods and products for their Plumbagin studies.
By improving reproducibility and accuracy, this platform can support the development of novel Plumbagin-based therapies and broaden our understanding of this fascinating naphthoquinone compound.