The core particles were prime-coated with pD by incubating 0.5 mg particles in 1 mL of dopamine hydrochloride solution in Tris buffer (10 mM, pH 8.5) for 3h at room temperature with rotation. Dopamine concentration was fixed at 0.5 mg/mL unless specified otherwise. The pD-coated PLGA particles (NP-pD or MP-pD) were collected by centrifugation (NP-pD: 8,161 ×g for 20 min; MP-pD: 1,306 ×g for 10 min) at 4°C. For surface functionalization, NP-pD or MP-pD were resuspended in Tris buffer (10 mM, pH 8.5), which contained different ligands (Folate, mPEG-NH2, Fol-PEG-NH2, cRADyK, cRGDyK, or pCB). The final concentrations of particles and ligands were 1 mg/mL and 2 mg/mL, respectively. After 30 min incubation at room temperature with rotation, particles were collected by centrifugation and washed with deionized water once. The functionalized NPs or MPs were named as NP-pD-Fol, NP-pD-PEG, NP-pD-PEG-Fol, NP-pD-RAD, NP-pD-RGD, MP-pD-pCB, and MP-pD-PEG according to the ligand used for the functionalization.
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Polydopamine
Polydopamine
Polydopamine is a versatile bioinspired material derived from the self-polymerization of the catecholamine dopamine.
This adhesive and multifunctional polymer has gained widespread interest in various applications, including surface modification, nanomedicine, and catalysis.
Polydopamine exhibits excellent biocompatibility, strong adhesion to diverse substrates, and the ability to undergo further modification.
Its unique properties make it a promising candidate for a wide range of research and development endeavors.
The PubCompare.ai platform offers an AI-driven approach to enhance the reproducibility of Polydopamine research, enabling researchers to easily locate relevant protocols from literature, preprints, and patents, and utilize AI-powered comparisons to identify the most optimized Polydopamine protocols and products.
This streamlined workflow can help take Polydopamine studies to new heights and accelerate advancements in this exciting field of materials science.
This adhesive and multifunctional polymer has gained widespread interest in various applications, including surface modification, nanomedicine, and catalysis.
Polydopamine exhibits excellent biocompatibility, strong adhesion to diverse substrates, and the ability to undergo further modification.
Its unique properties make it a promising candidate for a wide range of research and development endeavors.
The PubCompare.ai platform offers an AI-driven approach to enhance the reproducibility of Polydopamine research, enabling researchers to easily locate relevant protocols from literature, preprints, and patents, and utilize AI-powered comparisons to identify the most optimized Polydopamine protocols and products.
This streamlined workflow can help take Polydopamine studies to new heights and accelerate advancements in this exciting field of materials science.
Most cited protocols related to «Polydopamine»
Centrifugation
Dopamine
Folate
Hydrochloride, Dopamine
Ligands
monomethoxypolyethylene glycol
Polylactic Acid-Polyglycolic Acid Copolymer
Tromethamine
PDMS substrates were prepared by mixing ten parts of silicone elastomer base with one part of curing agent (SYLGARD, Dow Corning, USA) and stirred. The mixture was then poured into well plates or culture dishes, degassed for 30 min in a vacuum oven to remove air bubbles, followed by heat-curing at 70 °C for 100 min. Surface modification was performed by immersing the native PDMS surface in dopamine solution (Sigma Aldrich, Singapore) prepared in 10 mM Tris-HCl (pH 8.5). PD concentration (0.000%w/v–0.100%w/v) and duration of coating (0–24 h) were varied to investigate the effect of these parameters on the stability of BMSC adhesion and proliferation. After PD coating, the surfaces were rinsed twice with 1X Phosphate Buffered Saline (PBS) to remove unattached PD molecules. Additional coating of 20 μg/ml collagen type 1 (Life Technologies, Singapore) was performed to evaluate the combinatorial effect of PD and collagen on BMSC behaviour. Lastly, the uncoated and PD-coated PDMS substrates were UV-sterilized for 1 h prior to cell culture.
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Cell Culture Techniques
Collagen
Collagen Type I
Dopamine
Hyperostosis, Diffuse Idiopathic Skeletal
Phosphates
Saline Solution
Silicone Elastomers
Tromethamine
Vacuum
Buffers
Hydrochloride, Dopamine
Hydrolysis
PER1 protein, human
polydopamine
Silicon
Sodium Chloride
sodium phosphate
Tromethamine
Trypsin
Acetate
Biotin
biotinyl N-hydroxysuccinimide ester
Buffers
Horseradish Peroxidase
Peroxide, Hydrogen
Phosphates
polydopamine
Pyrogallol
Silicon
Sodium Chloride
sodium phosphate
Streptavidin
Tromethamine
Photopolymerizable thiol-ene composition was prepared as a mixture of PETMP and TTT with 1:1 stoichiometric ratio of thiol to ene functional groups, containing 1 wt. % of DMPA. The reason we chose cleavage photoinitiator is that it gives higher quantum yield for the production of reactive radicals as compared to the hydrogen-transfer photoinitiators [20 (link)]. Photoinitiator was dissolved in a warm PETMP at 60 °C in an amber glass jar, then the calculated amount of TTT was added avoiding the direct day or artificial light; components were thoroughly mixed with a spatula. The clear colorless viscous mixtures were applied on flexible polyethylene terephthalate (PET) substrates (APLI paper S.A., product Ref. 10580) as a 100 µm thick layer via the Meyer rod coating method. The PET substrate (thickness 0.1 mm) side was suitable for inkjet printing and was used in the deposition process. The water contact angle (CA) for this substrate side was determined to be 30 ± 1°, which is significantly lower than for polydopamine-coated (CA = 49.8°) or carboxyl-group-modified (CA = 50.6°) PET [33 (link)], but close to the O2 plasma treated PET films (CA = 34 ± 1°) [34 (link)]. In another instance, deposition was performed on the polytetrafluoroethylene (PTFE) plate. Samples were cured simultaneously at the intensity of 1.64 mW/cm2 (wavelength: 254 nm) and 0.8 mW/cm2 (wavelength: 365 nm). After that, cured network was obtained, denoted as PETMP-TTT. Free-standing PETMP-TTT films were obtained by gently peeling the film from the PTFE plate.
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Amber
Cytokinesis
Hydrogen
Light
N,N-dimethyl-4-anisidine
Plasma
polydopamine
Polyethylene Terephthalates
Polytetrafluoroethylene
Sulfhydryl Compounds
Viscosity
Most recents protocols related to «Polydopamine»
Polydopamine nanocarriers are formed by the auto-polymerization of dopamine hydrochloride under appropriate conditions. Add 10 mL distilled water and 10 mL anhydrous ethanol into the same beaker and place them in a magnetic stirrer for stirring. During the stirring process, absorb 0.5 mL ammonia water and add it into the beaker, and continue to stir at constant temperature. Weight 100 mg dopamine hydrochloride powder, absorb 1 mL distilled water to dissolve it fully, add it to the beaker as soon as possible, keep the system stirred at a constant speed for 12 h, then centrifuge the beaker solution separately (12 000 rpm, 2 min), carefully remove the supernatant with a pipette, and repeat the operation for 5 to 7 times. After centrifugation, the supernatant was water transparent, indicating that the washing and purification of the polydopamine nanomaterial had been completed. After centrifugation, the precipitated polydopamine nanoparticles were used for subsequent curcumin load characterization and detection. The size distribution and polydispersity index of polydopamine nanoparticles were determined by dynamic light scattering (DLS) measurements (Nano ZS90, Malvern Instruments, UK).
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Dopamine hydrochloride (2 mg/mL) (Sigma-Aldrich, St. Louis, MO, USA) was polymerized to polydopamine (PDA) in Tris-HCl buffer (pH 8.5) [25 (link)]. The prepared dark PDA solution was stored at 4 °C for a maximum period of a month [12 (link)].
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The UV absorption spectrum of polydopamine has a broad spectrum, with absorption in the range of 400–800 nm, but no clear absorption peak, while curcumin has a maximum UV absorption peak at 425 nm. Therefore, the experiment was designed to calculate the loading effect of polydopamine nanocarriers on curcumin by measuring the change of UV absorption value at 425 nm wavelength of the loading system. Three beakers were taken, and 30 mL of curcumin working liquid (8 μg mL−1) was measured in two beakers, and 30 mL of distilled water was measured in the other beaker as the control group. The beakers were placed in a magnetic stirrer and stirred at a constant speed. Add 10 mg of the above-mentioned prepared polydopamine nanoparticles into three beakers and stir them fully and continuously in the curcumin working liquid system. Collect 4 mL of each experimental group every 1 h. After centrifugation, the supernatant is tested for 425 nm absorption value. The loading and loading percentage of polydopamine nanocarriers on ginger were calculated until there was no significant change in light absorption value measured for 3 consecutive times (loading plateau period). After that, the prepared PDA-Cur NPs were purified, the unstable load of curcumin was fully washed away for analysis, and then characterized and used for backup. For curcumin releasing measurement, PDA-Cur NPs was dialyzed in PBS buffer at pH 6.0 in dark and kept in a 37 °C water bath. At different time points (1, 2, 4, 8 and 16 h), curcumin released from PDA-Cur NPs was collected. The size distribution and polydispersity index of PDA-Cur NPs were determined by dynamic light scattering (DLS) measurements (Nano ZS90, Malvern Instruments, UK).
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BBR NPs were prepared as described above. Aqueous dopamine hydrochloride (PDA) (0.2 mg·mL−1) and Tris base solution (6 mg·mL−1) were added into the solution of BBR NP sequentially to obtain an alkaline environment at pH=8.5. Then, the Polydopamine-coated Berberine nanoparticles (PDA@BBR NPs) suspension was obtained by reacting for 6 h under magnetic stirring at room temperature. Finally, the product was purified by centrifugation at 13,000 r·min−1 for 15 min and washed three times with distilled water to remove the unloaded BBR.
The POSS nanoparticles were coated with polydopamine (PDA) as described by Kibar et al. [31 ]. 0.25 mg of synthesized POSS nanoparticles were dispersed in 2 mg/mL dopamine hydrochloric (DOPA-HCl, Sigma-Aldrich) containing 10 mL of Tris-Buffer (pH 8.5). The mixture was magnetically stirred for 6 h in a dark hood. The reaction solution changed the color from white to dark. After PDA coating, POSS nanoparticles were collected via centrifugation at 15,000 rpm for 2 min and washed with DI water several times. The collected particles were dried at 50∘C in a vacuum oven overnight.
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Top products related to «Polydopamine»
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Dopamine hydrochloride is a chemical compound used in laboratory settings. It is a crystalline powder that serves as a precursor for the synthesis of various compounds. The core function of dopamine hydrochloride is to provide a source of the neurotransmitter dopamine for research and analytical purposes.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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The S-4800 is a high-resolution scanning electron microscope (SEM) manufactured by Hitachi. It provides a range of imaging and analytical capabilities for various applications. The S-4800 utilizes a field emission electron gun to generate high-quality, high-resolution images of samples.
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Dopamine is a laboratory reagent used in various biochemical and analytical applications. It is a naturally occurring neurotransmitter that plays a crucial role in the human body. Dopamine is often used as a standard in the measurement and analysis of compounds with similar chemical structures and properties.
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The ESCALAB 250Xi is a high-performance X-ray photoelectron spectroscopy (XPS) system designed for surface analysis. It provides precise and reliable data for the characterization of materials at the nanoscale level.
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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Dopamine hydrochloride is a chemical compound used in laboratory settings. It is a crystalline solid that is soluble in water. Dopamine hydrochloride is commonly used as a reference standard or analytical reagent in various scientific applications.
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The Zetasizer Nano ZS90 is a dynamic light scattering (DLS) instrument designed for the measurement of particle size and zeta potential. It utilizes a 633 nm laser and a detection angle of 90 degrees to analyze the Brownian motion of particles in a sample. The instrument can measure particle sizes ranging from 0.3 nm to 10 μm and zeta potential values from -500 mV to +500 mV.
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Pluronic F-127 is a non-ionic, tri-block copolymer composed of polyethylene oxide (PEO) and polypropylene oxide (PPO) segments. It is a widely used surfactant and emulsifying agent in various pharmaceutical and biomedical applications.
More about "Polydopamine"
Polydopamine is a versatile bioinspired material derived from the self-polymerization of the neurotransmitter dopamine.
This adhesive and multifunctional polymer has gained widespread interest in various applications, including surface modification, nanomedicine, and catalysis.
Polydopamine exhibits excellent biocompatibility, strong adhesion to diverse substrates, and the ability to undergo further chemical modifications.
Dopamine hydrochloride, a salt form of dopamine, is commonly used as a precursor for the synthesis of polydopamine.
Fetal bovine serum (FBS) and penicillin/streptomycin are often used in cell culture studies involving polydopamine-coated substrates to ensure optimal cell growth and proliferation.
The unique properties of polydopamine, such as its strong adhesion and versatile functionalization, make it a promising candidate for a wide range of research and development endeavors in materials science and biomedical engineering.
The ESCALAB 250Xi X-ray photoelectron spectrometer and Zetasizer Nano ZS90 dynamic light scattering instrument are commonly used to characterize the chemical composition and physical properties of polydopamine, respectively.
To enhance the reproducibility of polydopamine research, the PubCompare.ai platform offers an AI-driven approach that enables researchers to easily locate relevant protocols from literature, preprints, and patents, and utilize AI-powered comparisons to identify the most optimized polydopamine protocols and products.
This streamlined workflow can help take polydopamine studies to new heights and accelerate advancements in this exciting field of biomimetic materials.
Polydopamine can also be used in conjunction with other materials, such as Pluronic F-127, a non-ionic surfactant, to create complex, multifunctional systems for various applications.
By leveraging the insights and tools provided by PubCompare.ai, researchers can streamline their polydopamine-related studies and drive innovation in this rapidly evolving field of materials science.
This adhesive and multifunctional polymer has gained widespread interest in various applications, including surface modification, nanomedicine, and catalysis.
Polydopamine exhibits excellent biocompatibility, strong adhesion to diverse substrates, and the ability to undergo further chemical modifications.
Dopamine hydrochloride, a salt form of dopamine, is commonly used as a precursor for the synthesis of polydopamine.
Fetal bovine serum (FBS) and penicillin/streptomycin are often used in cell culture studies involving polydopamine-coated substrates to ensure optimal cell growth and proliferation.
The unique properties of polydopamine, such as its strong adhesion and versatile functionalization, make it a promising candidate for a wide range of research and development endeavors in materials science and biomedical engineering.
The ESCALAB 250Xi X-ray photoelectron spectrometer and Zetasizer Nano ZS90 dynamic light scattering instrument are commonly used to characterize the chemical composition and physical properties of polydopamine, respectively.
To enhance the reproducibility of polydopamine research, the PubCompare.ai platform offers an AI-driven approach that enables researchers to easily locate relevant protocols from literature, preprints, and patents, and utilize AI-powered comparisons to identify the most optimized polydopamine protocols and products.
This streamlined workflow can help take polydopamine studies to new heights and accelerate advancements in this exciting field of biomimetic materials.
Polydopamine can also be used in conjunction with other materials, such as Pluronic F-127, a non-ionic surfactant, to create complex, multifunctional systems for various applications.
By leveraging the insights and tools provided by PubCompare.ai, researchers can streamline their polydopamine-related studies and drive innovation in this rapidly evolving field of materials science.