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Polyethylene glycol 2000

Polyethylene glycol 2000 is a versatile and widely used polymer with a molecular weight of approximately 2,000 daltons.
It has diverse applications in biomedical research, drug delivery, and tissue engineering due to its unique physicochemical properties.
PEG 2000 is known for its ability to improve the solubility, stability, and pharmacokinetics of various compounds.
Researchers can explore the power of this remarkable molecule and discover the best protocols and products through the AI-driven platform of PubCompare.ai.
This user-friendly tool offers intelligent comparisons of literature, preprints, and patents, helping optimize research and unleash the full potential of PEG 2000.
Experience seamnless protocol discovery and product selection today and elevate your research with the power of PEG 2000.

Most cited protocols related to «Polyethylene glycol 2000»

1
Faecal DNA Isolation and Purification
Cited 39 times
Faecal DNA was isolated and purified according to the literature, with minor modifications.49 The bacterial pellet was suspended and incubated with 15 mg/ml lysozyme (Sigma-Aldrich Co., LCC) at 37°C for 1 h in TE10. Purified achromopeptidase (Wako Pure Chemical Industries, Ltd) was added at a final concentration of 2000 units/ml and then incubated at 37°C for 30 min. The suspension was treated with 1% (wt/vol) sodium dodecyl sulphate and 1 mg/ml proteinase K (Merck Japan) and incubated at 55°C for 1 h. The lysate was treated with phenol/chloroform/isoamyl alcohol (Life Technologies Japan, Ltd). DNA was precipitated by adding ethanol and pelleted by centrifugation at 3,300 g at 4°C for 15 min. The DNA pellet was rinsed with 75% ethanol, dried, and dissolved in 10 mM Tris-HCl/1 mM EDTA (TE). DNA samples were purified by treating with 1 mg/ml RNase A (Wako Pure Chemical Industries, Ltd) at 37°C for 30 min and precipitated by adding equal volumes of 20% polyethylene glycol solution (PEG6000-2.5M NaCl). DNA was pelleted by centrifugation at 8,060 g at 4°C, rinsed with 75% ethanol, and dissolved in TE.
Kim S.W., Suda W., Kim S., Oshima K., Fukuda S., Ohno H., Morita H, & Hattori M. (2013). Robustness of Gut Microbiota of Healthy Adults in Response to Probiotic Intervention Revealed by High-Throughput Pyrosequencing. DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes, 20(3), 241-253.
Publication 2013
Bacteria Centrifugation Chloroform Edetic Acid Endopeptidase K Ethanol Feces isopentyl alcohol lysyl endopeptidase Muramidase Phenol Polyethylene Glycol 6000 Polyethylene Glycols Ribonucleases Sodium Chloride Sulfate, Sodium Dodecyl Tromethamine
2
Lipid-Polymer Hybrid Nanoparticle Fabrication
Cited 29 times
Lipid-polymer hybrid NPs were prepared via self-assembly of PLGA (poly (D,L-lactic-co-glycolic acid); Lactel, Pelham, AL), lecithin (soybean, refined, molecular weight: ~330 Da; Alfa Aesar, Ward Hill, MA), and DSPE-PEG (1,2-distearoyl-sn-glycero-3-phosphoethanolamine- N-carboxy (polyethylene glycol)2000); Avanti, Alabaster, AL) through a single-step nanoprecipitation method. Briefly, PLGA polymer was dissolved in acetonitrile with concentrations ranging from 1~5 mg/mL. Lecithin/DSPE-PEG (8.5/1.5, molar ratio) with a weight ratio of 15% to the PLGA polymer were dissolved in 4 wt% ethanol aqueous solution. The lecithin/DSPE-PEG solution was heated to 65°C to ensure all lipids were in liquid phase. The resulting PLGA solution was then added into the preheated lipid solution dropwise under gentle stirring. The mixed solution was vortexed vigorously for 3 minutes followed by gentle stirring for 2 hours at room temperature. The remaining organic solvent and free molecules were removed by washing the NP solution three times using an Amicon Ultra-4 centrifugal filter (Millipore, Billerica, MA) with a molecular weight cut-off of 10,000 Da. To prepare drug-encapsulated NPs, docetaxel (Sigma-Aldrich, St Louis, MO) with proper initial dosage was dissolved into the PLGA acetonitrile solution before the nanoprecipitation process. NP size (diameter, nm) and surface charge (zeta potential, mV) were obtained from three repeat measurements by Quasi-elastic laser light scattering with a ZetaPALS dynamic light scattering detector (15 mW laser, incident beam = 676 nm; Brookhaven Instruments Corporation, Holtsville, NY).
Zhang L., Chan J.M., Gu F.X., Rhee J.W., Wang A.Z., Radovic-Moreno A.F., Alexis F., Langer R, & Farokhzad O.C. (2008). Self-Assembled Lipid-Polymer Hybrid Nanoparticles: A Robust Drug Delivery Platform. ACS nano, 2(8), 1696-1702.
Publication 2008
1,2-distearoylphosphatidylethanolamine acetonitrile Alabaster DA10 Docetaxel Ethanol glycolic acid Hybrids Lecithin Lipids Molar Pharmaceutical Preparations Phosphatidylethanolamines Poly A polyethylene glycol 2000 Polylactic Acid-Polyglycolic Acid Copolymer Polymers Solvents Soybeans
3
Structural Basis of RCC1-Nucleosome Complex
Cited 25 times
The complex of Drosophila RCC1(2–422) and nucleosome core particles containing Xenopus core histones and the 147 bp Widom 601 sequence were purified by size exclusion chromatography and crystallized against 25 mM sodium acetate pH 5.5, 25 mM sodium citrate, 1 mM DTT and 6–7% polyethylene glycol monomethyl ether 2,000 (PEG MME 2,000) at 21°C. Crystals were soaked in 25 mM sodium acetate pH 5.5, 25 mM sodium citrate, 1 mM DTT, 5% ethanol, 10% PEG MME 2,000 containing increasing concentrations of polyethylene glycol 400 (0 to 24% in 2% increments) before flash cooling in liquid nitrogen. Diffraction data were collected using an ADSC Quantum 315 CCD detector at Advanced Photon Source’s NE-CAT beamline 24-ID-E, and the data was processed using the HKL-2000 program suite38 . The structure was solved by molecular replacement using Phaser software39 (link) and a search model containing Drosophila RCC1, the histone octamer with tails removed, and the 147 bp human αsatellite DNA, each treated as a rigid body. Crystallographic refinement was carried out using REFMAC540 (link) and PHENIX41 (link) together with manual model building in COOT42 (link). The structure was refined to 2.9 Å resolution with Rwork/Rfree of 17.49/21.55%. All molecular graphics were prepared using PyMOL43 .
Makde R.D., England J.R., Yennawar H.P, & Tan S. (2010). Structure of RCC1 chromatin factor bound to the nucleosome core particle. Nature, 467(7315), 562-566.
Publication 2010
Crystallography Drosophila Ethanol Histones Homo sapiens Human Body Molecular Sieve Chromatography monomethoxypolyethylene glycol Muscle Rigidity Nitrogen Nucleosomes Sodium Acetate Sodium Citrate Tail Xenopus laevis
4
Recombinant AAV Production and Purification
Cited 18 times
Recombinant AAVs were generated by triple transfection of 293T cells (ATCC) using polyethylenimine (PEI)46 . Viral particles were harvested from the media at 72 hrs post transfection and from the cells and media at 120 hrs. Cell pellets were resuspended in 10mM Tris with 2mM MgCl2, pH 8, freeze-thawed three times, and treated with 100 U/mL Benzonase (Epicentre) at 37°C for at least 1 hr. Viral media was concentrated by precipitation with 8% polyethylene glycol 8000 (Sigma-Aldrich) with 500 mM sodium chloride47 (link), resuspended in Tris-MgCl2, and then added to the lysates. The combined stocks were then adjusted to 500 mM NaCl, incubated at 37°C for 30 minutes, and clarified by centrifugation at 2000 × g. The clarified stocks were then purified over iodixanol (Optiprep, Sigma; D1556) step gradients (15%, 25%, 40% and 60%)48 (link). Viruses were concentrated and formulated in phosphate buffered saline (PBS). Virus titers were determined by measuring the number of DNaseI-resistant vg using qPCR with linearized genome plasmid as a standard46 .
For capsid library virus generation, two modifications were made to the above virus production protocol to reduce the production of mosaic capsids that could arise from the presence of multiple capsid sequences in the same cell. First, only 10 ng of the rAAV-Cap-in-cis library plasmid was transfected (per 150 mm dish) to increase the likelihood that most transfected cells only received one capsid variant sequence. Second, the virus was collected at 48 hrs (media) and 60 hrs (cells and media), rather than at 72 hrs and 120 hrs as described above, to minimize the secondary transduction of producer cells with rAAV library virus released into the medium.
Deverman B.E., Pravdo P.L., Simpson B.P., Kumar S.R., Chan K.Y., Banerjee A., Wu W.L., Yang B., Huber N., Pasca S.P, & Gradinaru V. (2016). Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology, 34(2), 204-209.
Publication 2015
Benzonase Capsid Capsid Proteins Centrifugation DNA Library Freezing Genome HEK293 Cells Hyperostosis, Diffuse Idiopathic Skeletal iodixanol Magnesium Chloride Pellets, Drug Phosphates Plasmids polyethylene glycol 8000 Polyethyleneimine Saline Solution Sodium Sodium Chloride Transfection Tromethamine Virion Virus
5
Recombinant AAV Production and Purification
Cited 18 times
Recombinant AAVs were generated by triple transfection of 293T cells (ATCC) using polyethylenimine (PEI)46 . Viral particles were harvested from the media at 72 hrs post transfection and from the cells and media at 120 hrs. Cell pellets were resuspended in 10mM Tris with 2mM MgCl2, pH 8, freeze-thawed three times, and treated with 100 U/mL Benzonase (Epicentre) at 37°C for at least 1 hr. Viral media was concentrated by precipitation with 8% polyethylene glycol 8000 (Sigma-Aldrich) with 500 mM sodium chloride47 (link), resuspended in Tris-MgCl2, and then added to the lysates. The combined stocks were then adjusted to 500 mM NaCl, incubated at 37°C for 30 minutes, and clarified by centrifugation at 2000 × g. The clarified stocks were then purified over iodixanol (Optiprep, Sigma; D1556) step gradients (15%, 25%, 40% and 60%)48 (link). Viruses were concentrated and formulated in phosphate buffered saline (PBS). Virus titers were determined by measuring the number of DNaseI-resistant vg using qPCR with linearized genome plasmid as a standard46 .
For capsid library virus generation, two modifications were made to the above virus production protocol to reduce the production of mosaic capsids that could arise from the presence of multiple capsid sequences in the same cell. First, only 10 ng of the rAAV-Cap-in-cis library plasmid was transfected (per 150 mm dish) to increase the likelihood that most transfected cells only received one capsid variant sequence. Second, the virus was collected at 48 hrs (media) and 60 hrs (cells and media), rather than at 72 hrs and 120 hrs as described above, to minimize the secondary transduction of producer cells with rAAV library virus released into the medium.
Deverman B.E., Pravdo P.L., Simpson B.P., Kumar S.R., Chan K.Y., Banerjee A., Wu W.L., Yang B., Huber N., Pasca S.P, & Gradinaru V. (2016). Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology, 34(2), 204-209.
Publication 2015
Benzonase Capsid Capsid Proteins Centrifugation DNA Library Freezing Genome HEK293 Cells Hyperostosis, Diffuse Idiopathic Skeletal iodixanol Magnesium Chloride Pellets, Drug Phosphates Plasmids polyethylene glycol 8000 Polyethyleneimine Saline Solution Sodium Sodium Chloride Transfection Tromethamine Virion Virus

Most recents protocols related to «Polyethylene glycol 2000»

1
Lipid Nanoparticle Preparation and Characterization
CNLs were prepared as described in Sakae et al. [30 (link)]. Briefly, dioleoylphosphatidylethanolamine, distearoylphosphatidylcholine, distearoylphosphatidylethanolamine-polyethylene glycol 2000, polyethylene glycol 750-N-octanoylsphingosine, and C6-ceramide were mixed in chloroform, dried to a thin film under nitrogen, and then hydrated by the addition of sterilized 0.9% NaCl solution at 60 °C with sonicating and vortexing. Lipid solutions were then extruded at 60 °C by passing through 100 nm polycarbonate filters. Size (80 nM) and charge (−7 mV) were validated using Malvern Zetasizer Nano (Malvern Panalytical, Malvern, UK).
Ohya Y., Ogiso Y., Matsuda M., Sakae H., Nishida K., Miki Y., Fox T.E., Kester M., Sakamoto W., Nabe T, & Kitatani K. (2024). Pronecroptotic Therapy Using Ceramide Nanoliposomes Is Effective for Triple-Negative Breast Cancer Cells. Cells, 13(5), 405.
Full text: Click here
Publication 2024
2
Cyclodextrin-PEG Hydrogel Synthesis
α-Cyclodextrin
was purchased from Sigma-Aldrich (C4642, ≥
98%). Polyethylene glycol 2000 was purchased from Sigma-Aldrich (Mn = 2000 Da for synthesis). Gelatin type A from
porcine skin (ref G2500) and methacrylic anhydride (ref 276685) were
purchased from Sigma-Aldrich.
Stampoultzis T., Rana V.K., Guo Y, & Pioletti D.P. (2024). Impact of Molecular Dynamics of Polyrotaxanes on Chondrocytes in Double-Network Supramolecular Hydrogels under Physiological Thermomechanical Stimulation. Biomacromolecules, 25(2), 1144-1152.
Full text: Click here
Publication 2024
3
Radiolabeled Lipid Nanoparticle Synthesis
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), cholesterol (chol), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-mPEG2000), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (DSPE-PEG2000-Mal), 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[amino(-polyethyleneglycol)-2000] (DSPE-PEG2000-NH2), and 1,2-dioleoyl-snglycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (DOPE-rho) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid was purchased (p-SCN-Bn-NOTA) from Macrocyclics (Dallas, TX, USA). Copper-64 was produced at the Korea Institute of Radiological and Medical Sciences (KIRAMS, Seoul, Republic of Korea) by 50-MeV cyclotron irradiation [56 (link)]. Chloroform, methanol, dimethylformamide (DMF), and triethylamine (TEA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cetuximab was purchased from Merck KGaA (Darmstadt, Germany). Amicon filters were purchased from Millipore (Billerica, MA, USA).
Jeong H.Y., Kang S.J., Kim M.W., Jeong I.H., Choi M.J., Jung C., Song I.H., Lee T.S, & Park Y.S. (2024). Development of PET Radioisotope Copper-64-Labeled Theranostic Immunoliposomes for EGFR Overexpressing Cancer-Targeted Therapy and Imaging. International Journal of Molecular Sciences, 25(3), 1813.
Full text: Click here
Publication 2024
4
Lipid-based Anti-microRNA103/107 Delivery
Anti-microRNA103/107 (5′-rUrCrArUrArGrCrCrCrUrGrUrArCrArArUrGrCrUrGrCrU-3′) and (5′-/5RhoR-XN/rUrCrArUrArGrCrCrCrUrGrUrArCrArArUrGrCrUrGrCrU-3′), namely anti-miR103/107, was synthesized by Tema Ricerca s.r.l. (Bologna, Italy). 1,2-dioleyl-3-dimethylammonium propane (DODAP), N-palmitoyl-sphingosine-1-succinyl[methoxy(polyethylene glycol)2000] (PEG2000-Cer16) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000]-Maleimide (DSPE-PEG-Mal) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium salt) were obtained by Avanti Polar Lipids. Disteroylphosphatidylcholine (DSPC) was kindly offered from Lipoid GmbH (Cam, Switzerland). Cholesterol (CHOL), human transferrin (Tf), ammonium ferrithiocyanate, sodium borate, sodium chloride, sodium citrate, sodium phosphate, HEPES, ethylenediaminetetraacetic acid (EDTA), citric acid, Sepharose G-25, and 2-iminothiolane (Traut’s reagent) were purchased from Sigma-Aldrich (USA). Ethanol and other solvents were obtained by Exacta Optech (Italy).
Cepparulo P., Cuomo O., Campani V., Vinciguerra A., Sisalli M.J., Nele V., Anzilotti S., Valsecchi V., Casamassa A., Brancaccio P., Scorziello A., De Rosa G., Annunziato L, & Pignataro G. (2024). Anti-miRNA103/107 encapsulated in transferrin-conjugated lipid nanoparticles crosses blood-brain barrier and reduces brain ischemic damage. Molecular Therapy. Nucleic Acids, 35(1), 102131.
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Publication 2024
5
Targeted Nanoparticle Delivery for Neurodegeneration

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Publication 2024

Top products related to «Polyethylene glycol 2000»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Cholesterol by Merck Group
Sourced in United States, Germany, United Kingdom, India, Japan, Sao Tome and Principe, China, France, Spain, Canada, Switzerland, Italy, Australia, Israel, Brazil, Belgium, Poland, Hungary, Macao
Cholesterol is a lab equipment product that measures the concentration of cholesterol in a given sample. It provides quantitative analysis of total cholesterol, HDL cholesterol, and LDL cholesterol levels.
3
Dspe peg2000 by Avanti Polar Lipids
Sourced in United States, Germany
DSPE-PEG2000 is a lipid conjugate compound composed of distearoylphosphatidylethanolamine (DSPE) and polyethylene glycol (PEG) with an average molecular weight of 2000 Da. It is a widely used material in the development of liposomal drug delivery systems and other biomedical applications.
4
Chloroform by Merck Group
Sourced in United States, Germany, United Kingdom, India, Italy, Spain, France, Canada, Switzerland, China, Australia, Brazil, Poland, Ireland, Sao Tome and Principe, Chile, Japan, Belgium, Portugal, Netherlands, Macao, Singapore, Sweden, Czechia, Cameroon, Austria, Pakistan, Indonesia, Israel, Malaysia, Norway, Mexico, Hungary, New Zealand, Argentina
Chloroform is a colorless, volatile liquid with a characteristic sweet odor. It is a commonly used solvent in a variety of laboratory applications, including extraction, purification, and sample preparation processes. Chloroform has a high density and is immiscible with water, making it a useful solvent for a range of organic compounds.
5
1 2 dipalmitoyl sn glycero 3 phosphocholine by Avanti Polar Lipids
Sourced in United States, United Kingdom
1,2-dipalmitoyl-sn-glycero-3-phosphocholine is a synthetic phospholipid commonly used as a model system for the study of lipid membranes and their properties. It has a molecular formula of C40H80NO8P and is composed of two palmitoyl fatty acid chains attached to a glycerol backbone, with a phosphocholine head group.
6
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
7
Cholesterol by Avanti Polar Lipids
Sourced in United States
Cholesterol is a lipid compound found in animal cells. It is a core component of cell membranes and is essential for various physiological processes. Avanti Polar Lipids offers high-purity cholesterol for use in research and laboratory applications.
8
1 2 distearoyl sn glycero 3 phosphoethanolamine n methoxy polyethylene glycol 2000 dspe peg2000 by Avanti Polar Lipids
Sourced in United States, China
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) is a lipid conjugate composed of a distearoyl phospholipid and a polyethylene glycol (PEG) moiety. The PEG component is covalently attached to the lipid head group, providing a hydrophilic polymer layer.
9
1 2 dioleoyl sn glycero 3 phosphocholine by Avanti Polar Lipids
Sourced in United States
1,2-dioleoyl-sn-glycero-3-phosphocholine is a synthetic lipid compound. It is a phospholipid that consists of two oleic acid chains attached to a glycerol backbone, with a phosphocholine headgroup.
10
1 2 distearoyl sn glycero 3 phosphocholine by Avanti Polar Lipids
Sourced in United States
1,2-distearoyl-sn-glycero-3-phosphocholine is a lipid molecule that is commonly used as a component in various research and laboratory applications. It is a synthetic phospholipid that belongs to the class of phosphatidylcholines. The core function of this compound is to serve as a model system for studying membrane properties and behaviors in a controlled environment.

More about "Polyethylene glycol 2000"

Polyethylene glycol, also known as PEG or polyethylene oxide (PEO), is a versatile and widely used polymer in the biomedical field.
PEG 2000, with a molecular weight of approximately 2,000 daltons, is a particularly popular variant due to its unique physicochemical properties.
This hydrophilic, non-toxic, and non-immunogenic polymer has diverse applications in drug delivery, tissue engineering, and biomedical research.
PEG 2000 is known for its ability to improve the solubility, stability, and pharmacokinetics of various compounds, including drugs, proteins, and nanoparticles.
It can be used to conjugate with active pharmaceutical ingredients (APIs) or incorporated into drug formulations to enhance their biopharmaceutical characteristics.
For example, PEG 2000 can be attached to the surface of liposomes, such as those composed of DSPE-PEG2000 and DOPC, to improve their circulation time and reduce uptake by the reticuloendothelial system.
In tissue engineering, PEG 2000 hydrogels have been explored for their versatility in supporting cell growth and facilitating the delivery of growth factors, cells, and other biomolecules.
These hydrogels can be designed with tunable mechanical properties and biodegradability by incorporating various crosslinkers, such as FBS or cholesterol.
Researchers can utilize the power of PEG 2000 and discover the best protocols and products through the AI-driven platform of PubCompare.ai.
This user-friendly tool offers intelligent comparisons of literature, preprints, and patents, helping optimize research and unleash the full potential of this remarkable molecule.
By exploring the wealth of information available, scientists can find the most suitable PEG 2000-based solutions for their specific research needs, ultimately advancing the field of biomedical engineering and drug development.
Experience seamless protocol discovery and product selection today and elevate your research with the power of PEG 2000.