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Polyethylene Glycol 6000

Polyethylene Glycol 6000 (PEG 6000) is a widely used polymer in biomedical and pharmaceutical applications.
It is a non-toxic, water-soluble, and biocompatible compound that has been extensively studied for its versatile properties.
PEG 6000 is commonly employed as a drug delivery vehicle, stabilizer, and excipient in formulations.
It can enhance the solubility, stability, and pharmacokinetics of various active pharmaceutical ingredients.
Additionally, PEG 6000 has been utilized in tissue engineering, cell culturing, and other biotechnological processes.
Researchers can leverage the power of PubCompare.ai to optimize their PEG 6000 research by accessing the most reproducible and accurate protocols from literature, preprints, and patents.
This AI-driven platfrom allows for the comparison of products and procedures, helping researchers find the best solution for their specific needs.

Most cited protocols related to «Polyethylene Glycol 6000»

SARS-CoV-2 RBD-ACE2 Complex Purification and Crystallization

Cited 63 times

To purify the RBD/ACE2 complex, human ACE2 and RBD were incubated together, and then the complex was purified on Superdex200 gel filtration chromatography. RBD/ACE2 crystals were grown in sitting drops at room temperature over wells containing 100 mM Tris (pH 8.5), 18-20% PEG 6000, and 100 mM NaCl. Crystals were soaked briefly in 100 mM Tris (pH 8.5), 30% PEG 6000, 100 mM NaCl, and 30% ethylene glycol before being flash-frozen in liquid nitrogen. X-ray diffraction data were collected at the Advanced Photon Source beamline 24-ID-E. The structure was determined by molecular replacement using the structure of human ACE2 complexed with SARS-CoV RBD as the search template (Protein Data Bank accession code 2AJF). Structure data and refinement statistics are shown in Extended Data Table.1.

Shang J., Ye G., Shi K., Wan Y., Luo C., Aihara H., Geng Q., Auerbach A, & Li F. (2020). Structural basis of receptor recognition by SARS-CoV-2. Nature, 581(7807), 221-224.

Publication 2020

ACE2 protein, human Freezing Gel Chromatography Glycol, Ethylene Homo sapiens Nitrogen Polyethylene Glycol 6000 Severe acute respiratory syndrome-related coronavirus Sodium Chloride Tromethamine X-Ray Diffraction

Faecal DNA Isolation and Purification

Cited 39 times

Faecal DNA was isolated and purified according to the literature, with minor modifications.⁴⁹ The bacterial pellet was suspended and incubated with 15 mg/ml lysozyme (Sigma-Aldrich Co., LCC) at 37°C for 1 h in TE10. Purified achromopeptidase (Wako Pure Chemical Industries, Ltd) was added at a final concentration of 2000 units/ml and then incubated at 37°C for 30 min. The suspension was treated with 1% (wt/vol) sodium dodecyl sulphate and 1 mg/ml proteinase K (Merck Japan) and incubated at 55°C for 1 h. The lysate was treated with phenol/chloroform/isoamyl alcohol (Life Technologies Japan, Ltd). DNA was precipitated by adding ethanol and pelleted by centrifugation at 3,300 g at 4°C for 15 min. The DNA pellet was rinsed with 75% ethanol, dried, and dissolved in 10 mM Tris-HCl/1 mM EDTA (TE). DNA samples were purified by treating with 1 mg/ml RNase A (Wako Pure Chemical Industries, Ltd) at 37°C for 30 min and precipitated by adding equal volumes of 20% polyethylene glycol solution (PEG6000-2.5M NaCl). DNA was pelleted by centrifugation at 8,060 g at 4°C, rinsed with 75% ethanol, and dissolved in TE.

Kim S.W., Suda W., Kim S., Oshima K., Fukuda S., Ohno H., Morita H, & Hattori M. (2013). Robustness of Gut Microbiota of Healthy Adults in Response to Probiotic Intervention Revealed by High-Throughput Pyrosequencing. DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes, 20(3), 241-253.

Publication 2013

Bacteria Centrifugation Chloroform Edetic Acid Endopeptidase K Ethanol Feces isopentyl alcohol lysyl endopeptidase Muramidase Phenol Polyethylene Glycol 6000 Polyethylene Glycols Ribonucleases Sodium Chloride Sulfate, Sodium Dodecyl Tromethamine

Efficient Purification and Infection of HBV

Cited 18 times

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Publication 2018

Cell Culture Techniques Cells Chromatography, Affinity Collagen DNA Viruses Heparin Hep G2 Cells Polyethylene Glycol 6000 Sucrose Sulfoxide, Dimethyl Ultracentrifugation

Structural Determination of SARS-CoV-2 3CLpro-Baicalein Complex

Cited 17 times

The purified SARS-CoV-2 3CLpro protein was concentrated to 7 mg/mL for crystallization. The protein was incubated 1 h with 10 mM baicalein before crystallization condition screening. Crystals of the complex were obtained at 20 °C by mixing equal volumes of protein-baicalein and a reservoir (16% PEG6000, 100 mM MES, pH 5.8, 3% DMSO) with a hanging-drop vapor diffusion method. Crystals were flash-frozen in liquid nitrogen in the presence of the reservoir solution supplemented with 20% glycerol. X-ray diffraction data were collected at beamline BL18U1 at the Shanghai Synchrotron Radiation Facility [25 ]. The data were processed with HKL3000 software packages [26 (link)]. The complex structure was solved by molecular replacement using the program PHASER [27 (link)] with a search model of PDB code 6LU7. The model was built using Coot [28 (link)] and refined with a simulated annealing protocol implemented in the program PHENIX [29 (link)]. The refined structure was deposited to the Protein Data Bank with the accession code listed in Supplementary Table S1. The complete statistics as well as the quality of the solved structure are also shown in Supplementary Table S1.

Su H.X., Yao S., Zhao W.F., Li M.J., Liu J., Shang W.J., Xie H., Ke C.Q., Hu H.C., Gao M.N., Yu K.Q., Liu H., Shen J.S., Tang W., Zhang L.K., Xiao G.F., Ni L., Wang D.W., Zuo J.P., Jiang H.L., Bai F., Wu Y., Ye Y, & Xu Y.C. (2020). Anti-SARS-CoV-2 activities in vitro of Shuanghuanglian preparations and bioactive ingredients. Acta Pharmacologica Sinica, 41(9), 1167-1177.

Publication 2020

baicalein Crystallization Diffusion Freezing Glycerin Nitrogen Polyethylene Glycol 6000 Proteins Radiation SARS-CoV-2 Sulfoxide, Dimethyl X-Ray Diffraction

Bacterial DNA Isolation Protocol

Cited 16 times

Bacteria and bacterial DNA were prepared as described previously with minor modifications.^{26 (link),27} In brief, the bacterial DNA was isolated by the enzymatic lysis method using lysozyme (Sigma-Aldrich Co. LCC., Tokyo, Japan) and achromopeptidase (Wako). The DNA samples were purified by treatment with RNase A (Wako), followed by precipitation with 20% PEG solution (PEG6000 in 2.5 M NaCl). The DNA was pelleted by centrifugation, rinsed with 75% ethanol, and dissolved in TE buffer.

Nishijima S., Suda W., Oshima K., Kim S.W., Hirose Y., Morita H, & Hattori M. (2016). The gut microbiome of healthy Japanese and its microbial and functional uniqueness. DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes, 23(2), 125-133.

Publication 2016

Bacteria Buffers Centrifugation DNA, Bacterial Enzymes Ethanol lysyl endopeptidase Muramidase Polyethylene Glycol 6000 Ribonuclease, Pancreatic Sodium Chloride

Most recents protocols related to «Polyethylene Glycol 6000»

Crystallization of Bacterial Adhesion Proteins

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Publication 2023

Bistris Buffers Cryoprotective Agents Culicidae Diffusion Glycol, Ethylene HEPES malonate morpholinopropane sulfonic acid Nitrogen polyethylene glycol 3350 Polyethylene Glycol 6000 Proteins Selenomethionine Sodium Sodium Acetate Sodium Chloride Staphylococcal Protein A Sulfate, Ammonium Tromethamine

Immune Complex Precipitation and Analysis

Whole sera were treated with polyethylene glycol (PEG) 6000 to a final concentration of 2.5% overnight at 4°C. Immune complexes were precipitated as previously described [19 (link)], by centrifugation at 2000g for 30 minutes at 4°C, resuspending pellets in 30μl of prewarmed PBS, and analysed using SDS-PAGE and western blotting.

Fehmi J., Davies A.J., Antonelou M., Keddie S., Pikkupeura S., Querol L., Delmont E., Cortese A., Franciotta D., Persson S., Barratt J., Pepper R., Farinha F., Rahman A., Canetti D., Gilbertson J.A., Rendell N.B., Radunovic A., Minton T., Fuller G., Murphy S.M., Carr A.S., Reilly M.R., Eftimov F., Wieske L., Teunissen C.E., Roberts I.S., Ashman N., Salama A.D, & Rinaldi S. (2023). Contactin-1 links autoimmune neuropathy and membranous glomerulonephritis. PLOS ONE, 18(3), e0281156.

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Publication 2023

Centrifugation Complex, Immune Pellets, Drug Polyethylene Glycol 6000 SDS-PAGE Serum

Purification of RnMBV1 Virus Particles

RnMBV1 particles were purified according to Chiba et al.’s [32 (link)] method. The R. necatrix W779 strain was grown at 25°C in Difco potato dextrose broth (PDB, Becton Dickinson, Sparks, MD, USA) in the dark. Approximately 35 g (wet weight) of the mycelia was harvested and ground into powder in the presence of liquid nitrogen. The virus particles were then extracted from the homogenates in 150 mL of 100 mM sodium phosphate, pH 7.0, through clarification with Vertrel XF (Du Pont-Mitsui Fluorochemicals Co., Tokyo) for potato dextrose agar (PDA)-cellophane cultures. After the addition of NaCl and PEG 6000 to the final concentrations of 1% (w/v) and 8% (w/v), respectively, the particles were pelleted by high-speed centrifugation. After an additional round of differential centrifugation, the RnMBV1 particles were purified through sucrose gradient 10%–40% (w/v) centrifugation at 70, 000 x g for 2 h. The virus-containing fractions were pelleted and resuspended in 100 μL of 50 mM sodium-phosphate buffer (pH 7.0).

Wang H., Salaipeth L., Miyazaki N., Suzuki N, & Okamoto K. (2023). Capsid structure of a fungal dsRNA megabirnavirus reveals its previously unidentified surface architecture. PLOS Pathogens, 19(2), e1011162.

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Publication 2023

Agar Buffers Cellophane Centrifugation Glucose Mycelium Nitrogen Polyethylene Glycol 6000 Powder Sodium Chloride sodium phosphate Solanum tuberosum Strains Sucrose Virion Virus

Phylogenetic and Expression Analysis of Soybean HXK Genes

The sequences of the GmHXKs proteins obtained from the G. max genome were aligned using DNAMAN 7.0 software to search for conserved domains by inspection using sites present in AtHXK1 as a reference. To compare evolutionary relationships, the putative HXKs from G. max, A. thaliana, Solanum lycopersicum, O. sativa and Nicotiana tabacum were used to construct the phylogenetic tree using MEGA-X with the neighbor-joining (NJ) method and 1,000 bootstrap replicates (Kumar et al., 2018 (link)). Expression data on GmHXK gene family members at different developmental stages and in different tissues under normal conditions were downloaded from the Soybase (https://www.soybase.org/). Data on differential expression for only 14 members were eventually obtained and used for subsequent analysis.
To analyze expression pattern of soybean seedlings under salt stress, soybean seedlings were grown in a growth chamber under greenhouse conditions of 28°C under a16-h light/8-h dark cycle. Three-week-old seedlings were treated with 0.5% NaCl (salt stress) or drought treatment (10%PEG 6000). The root samples of the seedlings were collected after treatment for 2-h, 8-h, 24-h, and 72-h. Then, different samples were frozen quickly in liquid nitrogen, and stored at −80°C for RNA extraction and analysis. Total RNA was isolated using the Plant RNA Kit (CWBIO, Beijing, China), and its concentration and purity were determined by Nanodrop2000 nucleic acid analyzer (Thermo, America). First-strand cDNA was synthesized from 0.5 µg of total RNA using the HiFi-MMLV cDNA Kit (CWBIO, Beijing, China), and then used as a template for qRT-PCR analysis using gene-specific primers (Supplementary Table S1). Data analysis of RT-qPCR was performed using 2^−ΔΔCT method (Livak and Schmittgen, 2001 (link)).

Chen S., Tian Z, & Guo Y. (2023). Characterization of hexokinase gene family members in Glycine max and functional analysis of GmHXK2 under salt stress. Frontiers in Genetics, 14, 1135290.

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Publication 2023

Aftercare Amino Acid Sequence Biological Evolution DNA, Complementary Droughts Family Member Freezing Gene Expression Genes Genome Lycopersicon esculentum Nicotiana tabacum Nitrogen Nucleic Acids Oligonucleotide Primers Plant Roots Polyethylene Glycol 6000 RNA, Plant Salt Stress Seedlings Sodium Chloride Soybeans Tissues

Stress and hormone response in sweetpotato

Cited 2 times

The salt-tolerant XuShu 22 seedlings were grown in 1/4 Hoagland solutions and used for all the stress and hormone treatments with three different biological replicates. The roots were immersed in 150 mM NaCl and 20% PEG6000 solutions, respectively, for salt and dehydration stress treatment, then root samples were collected. Seedlings were incubated at 4°C and 42°C, respectively, for cold and heat conditions, then leaves samples were harvested. And the leaves of seedlings were collected after spraying with 0.1 mM ABA or 2 mM SA solutions for phytohormone tests (Meng et al., 2018 (link)). All of the samples were collected at 0, 1, 12, 24, and 48 hours after each treatment.
Total RNA was extracted using RNA Extraction Kits (TianGen, Beijing, China) following the manufacturer’s instructions. PrimeScript reverse transcriptase with the gDNA Eraser (TaKaRa, Dalian, China) was used to reverse-transcribe 1 μg of each RNA sample. qRT-PCR tests were conducted using the CFX96TM Real-Time System as previously described (Bio-Rad, USA) (Meng et al., 2020a (link)), and the sweetpotato ARF gene (JX177359) was employed as an internal control (Park et al., 2012 (link)). All the qRT-PCR primers are provided in Supplementary Table S4.

Zhang C., Dong T., Yu J., Hong H., Liu S., Guo F., Ma H., Zhang J., Zhu M, & Meng X. (2023). Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance. Frontiers in Plant Science, 14, 1140727.

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Publication 2023

Biopharmaceuticals Cold Temperature Dehydration Genes Hormones Ipomoea batatas Oligonucleotide Primers Plant Growth Regulators Plant Roots Polyethylene Glycol 6000 RNA-Directed DNA Polymerase Seedlings Sodium Chloride

Top products related to «Polyethylene Glycol 6000»

Peg 6000 by Merck Group

Sourced in United States, Germany, India, France, Japan, Italy, Hungary, United Kingdom, Spain

PEG 6000 is a polyethylene glycol product with an average molecular weight of 6,000 g/mol. It is a white, waxy solid used as a non-ionic, water-soluble polymer in various laboratory applications.

Polyethylene glycol 6000 by Merck Group

Sourced in United States, Germany, India

Polyethylene glycol 6000 is a polymer compound used as a laboratory reagent. It is a water-soluble, non-toxic, and non-reactive material commonly employed in various scientific applications.

Trizol reagent by Thermo Fisher Scientific

Sourced in United States, China, Japan, Germany, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Netherlands, Belgium, Lithuania, Denmark, Singapore, New Zealand, India, Brazil, Argentina, Sweden, Norway, Austria, Poland, Finland, Israel, Hong Kong, Cameroon, Sao Tome and Principe, Macao, Taiwan, Province of China, Thailand

TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.

Sodium chloride (nacl) by Merck Group

Sourced in United States, Germany, United Kingdom, India, Italy, France, Spain, China, Canada, Sao Tome and Principe, Poland, Belgium, Australia, Switzerland, Macao, Denmark, Ireland, Brazil, Japan, Hungary, Sweden, Netherlands, Czechia, Portugal, Israel, Singapore, Norway, Cameroon, Malaysia, Greece, Austria, Chile, Indonesia

NaCl is a chemical compound commonly known as sodium chloride. It is a white, crystalline solid that is widely used in various industries, including pharmaceutical and laboratory settings. NaCl's core function is to serve as a basic, inorganic salt that can be used for a variety of applications in the lab environment.

Polyethylene glycol peg 6000 by Merck Group

Sourced in United States, United Kingdom

Polyethylene glycol (PEG) 6000 is a high molecular weight, water-soluble polymer. It is commonly used as a component in various laboratory applications, including gel electrophoresis, protein purification, and cell culture media. PEG 6000 has a molecular weight of approximately 6,000 g/mol and exhibits a range of physicochemical properties that make it suitable for these applications.

Lysozyme by Merck Group

Sourced in United States, Germany, United Kingdom, Japan, China, Poland, Ireland, Switzerland, Italy, Sao Tome and Principe, India, Canada, France, Belgium, Australia, Netherlands, Singapore, Denmark, Macao, Portugal

Lysozyme is an enzyme that catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan, which is a major component of the cell walls of gram-positive bacteria. This function makes lysozyme an effective antimicrobial agent.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Sodium hydroxide (naoh) by Merck Group

Sourced in Germany, United States, India, United Kingdom, Italy, China, Spain, France, Australia, Canada, Poland, Switzerland, Singapore, Belgium, Sao Tome and Principe, Ireland, Sweden, Brazil, Israel, Mexico, Macao, Chile, Japan, Hungary, Malaysia, Denmark, Portugal, Indonesia, Netherlands, Czechia, Finland, Austria, Romania, Pakistan, Cameroon, Egypt, Greece, Bulgaria, Norway, Colombia, New Zealand, Lithuania

Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.

Peg 6000 by Thermo Fisher Scientific

Sourced in United States, Belgium

PEG 6000 is a polyethylene glycol product with an average molecular weight of 6,000 Daltons. It is a white, waxy solid at room temperature. PEG 6000 is commonly used as a stabilizer, emulsifier, and lubricant in various laboratory applications.

Tween 20 by Merck Group

Sourced in United States, Germany, United Kingdom, Italy, France, China, Spain, Australia, Japan, India, Poland, Sao Tome and Principe, Switzerland, Macao, Belgium, Canada, Denmark, Israel, Mexico, Netherlands, Singapore, Austria, Ireland, Sweden, Argentina, Romania

Tween 20 is a non-ionic detergent commonly used in biochemical applications. It is a polyoxyethylene sorbitan monolaurate, a surfactant that can be used to solubilize and stabilize proteins and other biomolecules. Tween 20 is widely used in various laboratory techniques, such as Western blotting, ELISA, and immunoprecipitation, to prevent non-specific binding and improve the efficiency of these assays.

What are some common applications of Polyethylene Glycol 6000 (PEG 6000)?

Polyethylene Glycol 6000 (PEG 6000) has a wide range of applications in the biomedical and pharmaceutical fields. It is commonly used as a drug delivery vehicle, stabilizer, and excipient in formulations. PEG 6000 can enhance the solubility, stability, and pharmacokinetics of various active pharmaceutical ingredients. Additionally, it has been utilized in tissue engineering, cell culturing, and other biotechnological processes.

How can PEG 6000 be used to improve drug delivery?

PEG 6000 can help improve drug delivery by enhancing the solubility and stability of active pharmaceutical ingredients. By incorporating PEG 6000 into drug formulations, researchers can increase the bioavailability and efficacy of drugs, leading to improved therapeutic outcomes. PEG 6000 also has the abiltiy to prolong the circulatin half-life of drugs, which can optimize their pharmacokinetic profile.

What are some of the key benefits of using PEG 6000 in biomedical and pharmaceutical applications?

One of the major benefits of PEG 6000 is its non-toxic and biocompatible nature. As a water-soluble polymer, it is well-tolerated by the body and does not elicit adverse immune responses. PEG 6000 can also act as a stabilizing agent, helping to maintain the structural integrity and activity of sensitive biomolecules and pharmaceutical compounds. Additionally, PEG 6000's versatile properties make it a valuable excipient for formulation development, allowing for improved solubility, stability, and delivery of a wide range of active ingredients.

How can PubCompare.ai assist researchers working with PEG 6000?

PubCompare.ai is an AI-driven platform that can greatly assist researchers optimizing their PEG 6000 research. The platform allows you to screen protocol liteature more efficiently and leverage AI to pinpoint critical insights. PubCompare.ai can help researchers identify the most effective protocols related to Polyethylene Glycol 6000 for their specific research goals. The platform's AI-driven analysis can highlight key differences in protocol effectiveness, enabling researchers to choose the best option for reproducibility and accuracy.

Are there different types or variations of Polyethylene Glycol 6000?

Yes, there are several variations of Polyethylene Glycol 6000 available. The molecular weight can vary, with different PEG 6000 products having slightly different properties and characteristics. Some PEG 6000 formulations may also be modified or functionalized for specific applications, such as pegylated proteins or PEG-based hydrogels. Researchers should carefullly evaluate the different PEG 6000 options to determine the most suitable one for their particular application and needs.

More about "Polyethylene Glycol 6000"

Polyethylene Glycol 6000 (PEG 6000) is a versatile and widely used polymer in the biomedical and pharmaceutical industries.
This non-toxic, water-soluble, and biocompatible compound has been extensively studied for its diverse applications.
PEG 6000 is commonly employed as a drug delivery vehicle, stabilizer, and excipient in various formulations.
It can enhance the solubility, stability, and pharmacokinetics of active pharmaceutical ingredients (APIs), making it a valuable tool for drug development.
PEG 6000 has also found applications in tissue engineering, cell culturing, and other biotechnological processes.
Researchers can leverage the power of PubCompare.ai, an AI-driven platform, to optimize their PEG 6000 research by accessing the most reproducible and accurate protocols from literature, preprints, and patents.
This platform allows for the comparison of products and procedures, helping researchers find the best solution for their specific needs.
In addition to PEG 6000, related terms such as Polyethylene glycol (PEG) 6000, Lysozyme, FBS (Fetal Bovine Serum), Sodium hydroxide, and Tween 20 are also commonly encountered in PEG 6000 research and applications.
Researchers can utilize these terms to further explore the various aspects of PEG 6000 usage, including its interactions with other compounds, formulation development, and downstream processing.
By leveraging the insights gained from PubCompare.ai and the wealth of information available on PEG 6000 and its related terms, researchers can streamline their experiments, improve reproducibility, and ultimately drive advancements in biomedical and pharmaceutical research.