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> Chemicals & Drugs > Organic Chemical > Polyethyleneimine

Polyethyleneimine

Polyethylenimine (PEI) is a cationic, branched polymer widely used in various biomedical applications, including gene delivery, protein purification, and drug delivery.
PEI possesses a high cationic charge density, which allows it to efficiently condense and protect nucleic acids, making it a popular transfection agent.
Reseach on PEI often focuses on optimizing its physicochemical properties, such as molecular weight and degree of branching, to enhance its performance and minimize cytotoxicity.
PubCompare.ai's AI-driven platform can help researchers locate the best protocols from literature, preprints, and patents, allowing them to compare data and identify the most accurate and reproducible methods for their Polyethylenimine studies.

Most cited protocols related to «Polyethyleneimine»

1
Env Protein Expression in HEK293 and CHO Cells
The Env proteins from various env genes described above were expressed in wild type, adherent HEK293T cells or the 293F variant that is adapted for suspension cultures, or in CHO-K1 cells, essentially as described [25] (link), [27] (link), [46] (link), [64] (link). HEK293T and CHO-K1 cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (FCS), penicillin (100 U/ml), streptomycin (100 µg/ml), Glutamax (Invitrogen), non-essential amino acids (0.1 mM), sodium pyruvate (0.1 mM) and HEPES (0.1 mM). For gp140 trimer production, HEK293T or CHO-K1 cells were seeded at a density of 5.5×104/ml in a Corning Hyperflask. After 3 days, when the cells had reached a density of 1.0×106/ml, they were transfected using polyethyleneimine (PEI) as described elsewhere [65] (link). Briefly, PEI-MAX (1.0 mg/ml) in water was mixed with expression plasmids for Env and Furin [5] (link) in OPTI-MEM. For one Corning Hyperflask, 600 µg of Env plasmid, 150 µg of Furin plasmid and 3 mg of PEI-MAX were added in 550 ml of growth media. Culture supernatants were harvested 72 h after transfection. BG505 gp120 used in differential scanning calorimetry (DSC) experiments was produced in HEK293F cells using a protocol similar to that previously described [25] (link).
Sanders R.W., Derking R., Cupo A., Julien J.P., Yasmeen A., de Val N., Kim H.J., Blattner C., de la Peña A.T., Korzun J., Golabek M., de los Reyes K., Ketas T.J., van Gils M.J., King C.R., Wilson I.A., Ward A.B., Klasse P.J, & Moore J.P. (2013). A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies. PLoS Pathogens, 9(9), e1003618.
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Publication 2013
Amino Acids, Essential Calorimetry, Differential Scanning Cells CHO Cells Culture Media Fetal Bovine Serum FURIN protein, human Gene Products, Protein GP 140 HEPES HIV Envelope Protein gp120 Penicillins Plasmids Polyethyleneimine Proteins Pyruvate Sodium Streptomycin Transfection
2
Lentivirus Production and Purification
Lentiviruses were produced by transfecting the HEK293T cells with the pFUGW vectors expressing GFP and three helper plasmids (pVSVg, RRE, and REV)14 (link). The transfections were carried out using the Polyethylenimine (PEI) method with the ratio at PEI:pFUGW:pVSVg:RRE:REV = 24:3:1:2:2. The virus-containing medium was harvested 48 or 72 hours after transfection and subsequently pre-cleaned with a 3,000 g centrifuge and a 0.45 μm filtration (Millipore). The virus-containing medium was overlaid on a sucrose-containing buffer (50 mM Tris-HCl, pH 7.4,100 mM NaCl, 0.5 mM ethylene diamine tetra acetic acid [EDTA]) at a 4:1 v/v ratio and centrifuged at the indicated RCF at 4 °C. After centrifugation, the supernatant was carefully removed, and the tube was placed on the tissue paper for 3 minutes. Phosphate Buffered Saline (PBS) was added to the semi-dried tube for re-suspension, and then the tube was placed in the 4 °C fridge with a cover for recovery overnight.
Jiang W., Hua R., Wei M., Li C., Qiu Z., Yang X, & Zhang C. (2015). An optimized method for high-titer lentivirus preparations without ultracentrifugation. Scientific Reports, 5, 13875.
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Publication 2015
Acetic Acids Centrifugation Cloning Vectors ethylenediamine Filtration Lentivirus Phosphates Plasmids Polyethyleneimine Saline Solution Sodium Chloride Sucrose Tetragonopterus Tissues Transfection Tromethamine Virus
3
Generation of SARS-CoV-2 Pseudotype Particles
To generate (HIV/NanoLuc) SARS-CoV-2 pseudotype particles, 5 × 106 293T cells were plated per 10-cm dish in 10 ml in growth medium. The following day, 7.5 µg pHIV-1NL4-3 ΔEnv-NanoLuc reporter virus plasmid and 2.5 µg SARS-CoV-2 or SARS-CoV plasmid (unless otherwise indicated, pSARS-CoV-2-SΔ19 was used) were mixed mix thoroughly with 500 µl serum-free DMEM (this represents a molar plasmid ratio of 1:0.55). Then, 44 µl polyethylenimine (PEI; 1 mg/ml) was diluted in 500 µl serum-free DMEM and mixed thoroughly.
To generate control virus lacking S, the S expression plasmid was omitted from the transfection, and the amount of PEI was reduced to 30 µl. The diluted DNA and PEI were then mixed thoroughly by pipetting or vortexing, incubated at 20 min at room temperature (RT), and added dropwise to the 293T cells. After 8-h or overnight incubation, the transfected cells were washed carefully twice with PBS and incubated in 10 ml DMEM++. At 48 h after transfection, the 10-ml supernatant was harvested, clarified by centrifugation at 300 g for 5 min, and passed through a 0.22-µm pore-size polyvinylidene fluoride syringe filter (Millipore; SLGVR33RS), aliquoted, and frozen at −80°C.
Schmidt F., Weisblum Y., Muecksch F., Hoffmann H.H., Michailidis E., Lorenzi J.C., Mendoza P., Rutkowska M., Bednarski E., Gaebler C., Agudelo M., Cho A., Wang Z., Gazumyan A., Cipolla M., Caskey M., Robbiani D.F., Nussenzweig M.C., Rice C.M., Hatziioannou T, & Bieniasz P.D. (2020). Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses. The Journal of Experimental Medicine, 217(11), e20201181.
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Publication 2020
Cells Centrifugation Culture Media Freezing HEK293 Cells Hyperostosis, Diffuse Idiopathic Skeletal Molar nanoluc Plasmids Polyethyleneimine polyvinylidene fluoride SARS-CoV-2 Serum Severe acute respiratory syndrome-related coronavirus Syringes Transfection Virus
4
Generation and Purification of AAV Particles
AAVs were generated in HEK 293T cells (ATCC) using Polyethylenimine (PEI)61 (link). 72 hours post transfection, viral particles were harvested from the media and after 120 hours from cells and the media. Viral particles from the media were precipitated with 40% polyethylene glycol (Sigma, 89510-1KG-F) in 500 mM NaCl and combined with cell pellets for processing. The cell pellets were suspended in 500 mM NaCl, 40 mM Tris, 2.5 mM MgCl2, pH 8, and 100 U/mL of salt-activate nuclease (Arcticzymes) at 37°C for 30 minutes. Afterwards, the cells were clarified by centrifugation at 2,000 × g and then purified over iodixanol (Optiprep, Sigma; D1556) step gradients (15%, 25%, 40%, and 60%)62 (link). Viruses were concentrated using Amicon filters (EMD, UFC910024), and formulated in sterile phosphate buffer saline (PBS). Virus titers were measured by determining the number of DNAse I-resistant vg using qPCR using a linearized genome plasmid as a standard61 (link).
Chan K.Y., Jang M.J., Yoo B.B., Greenbaum A., Ravi N., Wu W.L., Sánchez-Guardado L., Lois C., Mazmanian S.K., Deverman B.E, & Gradinaru V. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems. Nature neuroscience, 20(8), 1172-1179.
Publication 2017
Buffers Cells Centrifugation Deoxyribonuclease I F 500 Genome HEK293 Cells iodixanol Magnesium Chloride Pellets, Drug Phosphates Plasmids Polyethylene Glycols Polyethyleneimine Saline Solution Sodium Chloride Sterility, Reproductive Transfection Tromethamine Virion Virus
5
Ligand Binding and Uptake Assays for SERT
Ligand binding experiments were carried out by adding HEK293 membranes containing SERT to a final concentration of 2 nM in 1 ml of TBS with either [3H]paroxetine 0.01–10 nM or [3H](R/S)-citalopram 0.01–20 nM. Reactions were rotated at room temperature for 4 hours followed by filtering through a glass microfiber filter prewet with 0.4% polyethylenimine in TBS. Membranes were washed 3x with 4 ml of TBS followed by liquid scintillation counting. Data was fit to a single-site binding curve accounting for ligand depletion. For dissociation, 20 nM SERT in membranes was mixed with 40 nM [3H](R/S)-citalopram in 10 μl; samples were diluted to 1 ml in TBS with 100 μM (S)-citalopram, or without ligand, followed by filtering. For uptake assays, ~105 HEK293 cells in 96-well Cytostar T plates were transfected with 0.2 μg of plasmid with Polyjet. After 24–36 hrs, cells were washed with 25 mM HEPES-Tris pH 7.0, 130 mM NaCl, 5.4 mM KCl, 1.2 mM CaCl2, 1.2 mM MgSO4, 1 mM ascorbic acid, and 5 mM glucose. For a control, 10 μM paroxetine was added. [14C]5-hydroxytryptamine at concentrations of 0.02–40 μM was added and uptake was followed using a MicroBeta scintillation counter. Data was fit to a Michaelis-Menten equation.
Coleman J.A., Green E.M, & Gouaux E. (2016). X-ray structures and mechanism of the human serotonin transporter. Nature, 532(7599), 334-339.
Publication 2016
Ascorbic Acid Binding Sites Biological Assay Cells Citalopram Escitalopram Glucose HEK293 Cells HEPES Ligands Paroxetine Plasmids Polyethyleneimine Scintillation Counters Serotonin Sodium Chloride Sulfate, Magnesium Tissue, Membrane Tromethamine

Most recents protocols related to «Polyethyleneimine»

1
Amino-Functionalized Polyester Adhesives
Not available on PMC !

Example 4

As shown in Table 5a herein below, two (2) adhesive formulations (AF5-AF6) were prepared by independently mixing two of the above defined amino functionalized polyesters (Table 1: NH2B, NH2F) with 40 mol. % polyethylenimine (MW 800) and then with a cyclic carbonate functional curing partner (CC-BADGE): said curing partner was obtained by the addition of CO2 to the epoxide compound. The mixtures were prepared at a ratio of [Cyclic Carbonate groups]:[Primary and Secondary Amino groups] of 1:1 and were cured at 130° C. for 20 hours.

TABLE 5a
Adhesive Formulations
AdhesiveNH-PES with PEICC-BADGE
FormulationIdentity of NH-PES(g)(g)
AF-52B1.1141.386
AF-62F1.4781.022

Utilizing these adhesive formulations, lap shear tests were performed according to DIN EN 1465 and using the following substrates: ARMS-ARMS; and, Beech Wood-Beech Wood. The results of the tests performed at room temperature are shown in Table 5b herein below, wherein: SF means substrate failure; AF means adhesive failure; CF means cohesive failure; and, NM means not measured.

TABLE 5b
Lap Shear Strength
Beech
AdhesiveCuringARMSWood
FormulationConditions(MPa)(MPa)
AF-5130° C., 201.362.56
hoursAFCF
AF-62.75NM
AF

US11866547B2. Method for producing functionalized polyesters (2024-01-09). Henkel AG & Co. KGaA [DE]. Inventors: Sonia Flores Penalba [ES], Miguel Paradas-Palomo [ES], Jose Garcia Miralles [DE], Hans-Georg Kinzelmann [DE], Rosa Maria Sebastian Perez [ES], Jordi Marquet Cortes [ES], Jorge Aguilera Corrochano [ES], Federica Arioli [ES], Therese Hemery [DE].
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Patent 2024
AF 75 Arm, Upper Beech Carbonates Epoxy Compounds Polyesters Polyethyleneimine Shear Strength
2
Lignosulfonate-Polyamine Composite Synthesis
Not available on PMC !

Example 1

Calcium lignosulfonate (Borrement CA 2120) was provided by Borregaard LignoTech. Sodium lignosulfonate was purchased from Aldrich, and ammonium lignosulfonate was obtained from TemBac.

Sodium carboxymethylcellulose (NaCMC), hydroxypropylcellulose (HPC) and hydroxyethylcellulose (HEC) were obtained from Aldrich and showed a Mw of approx. 250 kDa, 100 kDa and 100 kDa, respectively.

The amine functional material such as hexamethylene diamine (HMDA) and diethylenetriamine (DETA) were obtained from Aldrich. Different types of polyethylenimines (Lupasol® EO, Lupasol® PS, Lupasol® P and Lupasol® G100), polyvinyl amines (Luredur® VM, Luredur® VH and Luredur® VI), were obtained from BASF Chemical Company, and polyetheramines (JeffamineED600, JeffamineEDR148, JeffamineT403) from Huntsman Holland BV.

The required amounts of polymer and lignosulfonate (LS) were dissolved in water individually. The required amount of polyamine functional compound was added to the LS solution followed by homogenization. The polymer solution and LS-amine solution were then combined at ambient temperature and stirred at 500 rpm for 30 minutes.

US11879063B2. Binder compositions and uses thereof (2024-01-23). KNAUF INSULATION SPRL [BE], KNAUF INSULATION, INC. [US]. Inventors: Carl Hampson [GB], Ferdous Khan [GB].
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Patent 2024
Amines Ammonium calcium lignosulfonate Cyclohexane Diamines diethylenetriamine hydroxyethylcellulose hydroxypropylcellulose lignosulfonates Polyamines Polyethyleneimine Polymers Polyvinyls Sodium Sodium Carboxymethylcellulose TLR4 agonist G100
3
Lentiviral Transduction of Myeloma Cells
Transient transfections to HEK 293 T cells were performed using polyethyleneimine (PEI) (Polysciences, Warrington, PA, USA) in the OPTI-MEM medium (Life Technologies, Carlsbad, CA, USA) with a ratio of 1:4 to 1:6 of DNA: PEI.
Viral particles were produced by HEK 293 T cells in a 10 cm dish transfected with 4 μg pMD2.G and 6 μg psPAX2 packaging plasmids (Addgene, Watertown, MA, USA), together with 8 μg lentiviral expressing vectors encoding target genes, including pITA -CBX3-N/C-flag, pLKO.1 or hU6-MSC-Ubiquitin-eGFP vector encoding shRNAs targeting interested genes as listed in the Supplementary Information. Supernatant carrying the viral particles was harvested 35 h and 60 h after transfection and concentrated to 100× volume by Poly (ethylene glycol) 8000 (Sigma-Aldrich, St. Louis, MS, USA).
For viral infection, 1 × 106 myeloma cells were seeded in 1 ml new complete media for 6 h and then added 50 μl viral concentration and 8 μg/mL polybrene, and cells were spin at 800 × g for 45 min at 20 °C. In total, 12 h after spinfection, the medium was changed and cells were cultured for another 48 h until further management.
Li X., Wang S., Xie Y., Jiang H., Guo J., Wang Y., Peng Z., Hu M., Wang M., Wang J., Li Q., Wang Y, & Liu Z. (2023). Deacetylation induced nuclear condensation of HP1γ promotes multiple myeloma drug resistance. Nature Communications, 14, 1290.
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Publication 2023
Cells Cloning Vectors G-800 Genes HEK293 Cells Hyperostosis, Diffuse Idiopathic Skeletal Multiple Myeloma Plasmids Polybrene polyethylene glycol 8000 Polyethyleneimine Short Hairpin RNA Transfection Transients Ubiquitin Virion Virus Diseases
4
HEK293 Cell Culture and Transfection
HEK293 cells were grown in DMEM (Wisent, 319-005-CL) supplemented with 10% fetal calf serum (Sigma, F1051) at 37 °C under 5% CO2. Cells were seeded into 15 cm plates and co-transfected with NanoLuc plasmids using linear polyethylenimine 25 kDa (PEI 25 K; Polysciences, 23966). Cells were harvested after 24 h and cell pellets were stored at − 80 °C.
Alexander-Howden B., Zhang L., van der Sloot A.M., Tollis S., St-Cyr D.J., Sicheri F., Bird A.P., Tyers M, & Lyst M.J. (2023). A screen for MeCP2-TBL1 interaction inhibitors using a luminescence-based assay. Scientific Reports, 13, 3868.
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Publication 2023
Cells Fetal Bovine Serum HEK293 Cells nanoluc Pellets, Drug Plasmids Polyethyleneimine
5
HEK293 Cell Transfection and Microscopy
HEK293 cells (CRL-1573, A.T.C.C., Manassas, VA, USA) were grown in 25 cm2 culture flasks in MEM-Earle’s media (Sigma-Aldrich, Munich, Germany) supplemented with 10% (v/v) fetal bovine serum, 100 U/mL penicillin, and 100 µg/ml streptomycin (Sigma-Aldrich), at 37 °C in a humidified 5% CO2 atmosphere. Cell line identity was confirmed by PCR single locus technology (Promega, PowerPlex 21 PCR Kit; Eurofins Medigenomix Forensik GmbH, Ebersberg, Germany) in 2018.
For transient transfection, HEK293 cells were seeded on poly-L-lysine (Sigma–Aldrich) coated coverslips in 6-well plates, at a density of 600,000 cells per well. 24 h later, cells were transfected by adding 500 µl cell culture medium (without supplements) per well, containing 10 µl polyethylenimine solution (Sigma-Aldrich, stock concentration 1 µg/µl), 2 µg EYFP- and 2 µg ECFP-plasmid DNA. Approximately 48 h after transfection, HEK293 cells were used for confocal laser scanning microscopy.
The following plasmid combinations were used: EYFP-Cldn10b wt–ECFP-Cldn10b wt, EYFP-Cldn10b fs–ECFP-Cldn10b fs, EYFP-Cldn10b wt–ECFP-Cldn10b fs, EYFP-Cldn10b fs–ECFP- Cldn10b wt. For comparison, the frameshift mutant previously found in Saudi Arabian HELIX syndrome patients37 (link) was also included: EYFP-Cldn10b fs(SA)–ECFP-Cldn10b wt.
Wuttke M., König E., Katsara M.A., Kirsten H., Farahani S.K., Teumer A., Li Y., Lang M., Göcmen B., Pattaro C., Günzel D., Köttgen A, & Fuchsberger C. (2023). Imputation-powered whole-exome analysis identifies genes associated with kidney function and disease in the UK Biobank. Nature Communications, 14, 1287.
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Publication 2023
Atmosphere Cell Culture Techniques Cell Lines Cells Culture Media Dietary Supplements Fetal Bovine Serum Frameshift Mutation HEK293 Cells Helix (Snails) Lysine Microscopy, Confocal Penicillins Plasmids Poly A Polyethyleneimine Promega Streptomycin Syndrome Transfection Transients

Top products related to «Polyethyleneimine»

1
Polyethylenimine (pei) by Polysciences
Sourced in United States, Germany, United Kingdom, Panama, China, Canada, Switzerland
PEI (Polyethylenimine) is a cationic polymer used as a transfection reagent in cell biology and molecular biology applications. It facilitates the delivery of nucleic acids, such as DNA or RNA, into cells for various experimental purposes.
2
Polyethylenimine (pei) by Merck Group
Sourced in United States, Germany, China, Canada, United Kingdom, Sao Tome and Principe, Israel, Spain, Switzerland, France, Macao
PEI is a type of laboratory equipment used in various scientific applications. It functions as a polymeric transfection reagent, facilitating the delivery of nucleic acids, such as DNA or RNA, into cells for research purposes. The core function of PEI is to enable efficient and controlled cellular uptake of these genetic materials.
3
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
4
Lipofectamine 2000 by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Italy, Switzerland, Australia, Spain, Belgium, Denmark, Singapore, India, Netherlands, Sweden, New Zealand, Portugal, Poland, Israel, Lithuania, Hong Kong, Argentina, Ireland, Austria, Czechia, Cameroon, Taiwan, Province of China, Morocco
Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
5
Opti mem by Thermo Fisher Scientific
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Opti-MEM is a cell culture medium designed to support the growth and maintenance of a variety of cell lines. It is a serum-reduced formulation that helps to reduce the amount of serum required for cell culture, while still providing the necessary nutrients and growth factors for cell proliferation.
6
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
7
Polybrene by Merck Group
Sourced in United States, Germany, China, Sao Tome and Principe, United Kingdom, Macao, Canada, France, Japan, Switzerland, Italy, Australia, Netherlands, Morocco, Israel, Ireland, Belgium, Denmark, Norway
Polybrene is a cationic polymer used as a transfection reagent in cell biology research. It facilitates the introduction of genetic material into cells by enhancing the efficiency of DNA or RNA uptake.
8
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
9
Polyethyleneimine by Merck Group
Sourced in United States, Germany, Poland, India, France, China, Sao Tome and Principe, United Kingdom
Polyethyleneimine is a water-soluble, cationic polymer that is commonly used as a transfection agent in molecular biology and cell culture applications. It functions as a non-viral vector for the delivery of nucleic acids into eukaryotic cells.
10
Pspax2 by Addgene
Sourced in United States, Switzerland, China, Japan, Germany, United Kingdom, France, Canada
PsPAX2 is a packaging plasmid used for the production of lentiviral particles. It contains the necessary genes for lentiviral packaging, but does not contain the viral genome. PsPAX2 is commonly used in lentiviral production workflows.

More about "Polyethyleneimine"

Polyethyleneimine (PEI) is a cationic, branched polymer that has become a widely used tool in various biomedical applications, including gene delivery, protein purification, and drug delivery.
With its high cationic charge density, PEI is able to efficiently condense and protect nucleic acids, making it a popular transfection agent.
Researchers often focus on optimizing PEI's physicochemical properties, such as molecular weight and degree of branching, to enhance its performance and minimize cytotoxicity.
PubCompare.ai's AI-driven platform can be a valuable resource for researchers working with PEI.
The platform helps locate the best protocols from literature, preprints, and patents, allowing users to compare data and identify the most accurate and reproducible methods for their PEI studies.
This can be particularly helpful when working with related materials like fetal bovine serum (FBS), Lipofectamine 2000, Opti-MEM, DMEM, Polybrene, and Penicillin/Streptomycin, which are commonly used in PEI-based experiments.
By utilizing PubCompare.ai, researchers can streamline their PEI research and ensure they are using the most effective and reliable protocols, leading to more accurate and reproducible results.
This can be especially beneficial for studies involving the optimization of PEI's physicochemical properties, as the platform can help identify the best approaches for enhancing PEI's performance while minimizing potential cytotoxicity.