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Potassium Oxalate

Potassium Oxalate: A versatile chemical compound with a variety of applications in scientific research and industrial processes.
Potassium oxalate is a salt of oxalic acid and potassium, known for its unique properties and uses.
This MeSH term provides a concise overview of the substance, highlighting its key characteristics and potential areas of study, such as in the field of Potassium Oxalate research.
Researchers can utilize this description to gain a quick, informative understanding of this important chemical compound and its relevance to their work.

Most cited protocols related to «Potassium Oxalate»

Anthropometric measurements, including weight, height, hip, and WC were measured with the subjects wearing light clothing and no shoes according to the World Health Organization report.29 WC was measured to the nearest centimeter using a nonstretchable tailors’ measuring tape at the midpoint between the bottom of the rib cage and above the top of the iliac crest during minimal respiration. Hip circumference was measured at the widest part of the body below the waist. Waist and hip circumferences were measured using a circumference measuring tape (Seca 200, Hamburg, Germany). BMI was calculated as the ratio of weight in kilograms to the square of height in meters. Readings of systolic and diastolic blood pressure were taken with the subject seated and the arm at heart level, after at least five minutes of rest, using a standardized mercury sphygmomanometer.30
For laboratory analysis and all biochemical measurements, two sets of fasting blood samples were drawn from a cannula inserted in the antecubital vein and put into sodium fluoride potassium oxalate tubes for glucose and into lithium heparin vacuum tubes for lipids. Samples were centrifuged at 3000 rpm for 10 minutes within one hour at the survey site, and plasma was transferred to separate labeled tubes and transferred immediately in cold boxes filled with ice to the Central Laboratory of the National Center for Diabetes and Endocrinology. All biochemical measurements were carried out by the same team of laboratory technicians and using the same method throughout the study period.
Lipid parameters, ie, total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglyceride (TG), and glucose were analyzed for all samples using enzymatic assays. Glucose levels were determined using the enzymatic reference method with hexokinase.31 (link) TG values were obtained using COBAS Integra 700 (Roche Diagnostics Ltd, Indianapolis, IN) with the cassette COBAS Integra TG (Roche Diagnostics Ltd) using an enzymatic colorimetric method with glycerol phosphate oxidase and 4-aminophenazone.32 (link) Total cholesterol was analyzed using an enzymatic colorimetric method with COBAS Integra Cholesterol Gen.2 (Roche Diagnostics Ltd). HDL and LDL values were obtained on COBAS Integra 700 using a homogeneous enzymatic colorimetric assay.33 (link),34 (link) The assays were conducted according to the manufacturer’s instructions.
Publication 2010
Ampyrone Biological Assay BLOOD Cannula Cell Respiration Cholesterol Cold Temperature Colorimetry Diabetes Mellitus Diagnosis Enzyme Assays Enzymes Glucose Heart Heparin Hexokinase High Density Lipoproteins Iliac Crest L-alpha-glycerol-phosphate oxidase Laboratory Technicians Light Lipids Lithium Low-Density Lipoproteins Mercury Plasma potassium fluoride Potassium Oxalate Pressure, Diastolic Rib Cage Sodium Fluoride Sodium Oxalate Sphygmomanometers System, Endocrine Systole Triglycerides Vacuum Veins
Each horse underwent an incremental high-speed treadmill test (Sato I, Uppsala, Sweden). Two days before the test, the horses were conditioned to the treadmill with two daily sessions, and the test was performed on the third day. The belt was inclined with a slope of 5% and the protocol consisted of a warm up of 4 min walk (1.5 m/s) and 3 min trot (6 m/s), followed by 1 min phases increasing the speed of 1 m/s for each stage, until the horse was no longer able to maintain the treadmill speed (maximal speed 12 m/s). Cool down was achieved by 30 min walk with 0% slope [19 (link)].
Blood samples during the test were taken without stopping the treadmill with the aid of a 14 G Teflon venous catheter placed in the jugular vein connected to an extension tube. Blood samples were taken at rest, after the warm-up, just before increasing the speed at the end of each phase, and at 5, 10 and 30 min during the recovery phase. After collection, blood was transferred into tubes containing 10 mg of sodium fluoride and 2 mg of potassium oxalate for 1 mL of blood. Within 15 min, the samples were centrifuged (4000 g for 10 min) to obtain plasma, refrigerated (2–8 °C) and analyzed. Plasma lactate (mmol/L) was measured using the enzymatic colorimetric method with a lactate dry-fast kit for the automatic system (Uni Fast System II Analyzer, Sclavo, Italy) and reagents supplied by the manufacturer.
Data concerning the values of plasma lactate at each speed stage were collected on an electronic sheet (Microsoft Excel, Redmond, USA) and VLA4 was calculated for each horse with dedicated software (Lactate-E) [20 (link)]. This was calculated using inverse prediction by finding the speed corresponding to a lactate value equal to 4 mmol/L.
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Publication 2020
BLOOD Catheters Colorimetry Enzymes Equus caballus Hematologic Tests Integrin alpha4beta1 Jugular Vein Lactates Plasma Potassium Oxalate Sodium Fluoride Teflon Treadmill Test Veins
Prior to study enrollment, potential participants completed a detailed electronic medical history questionnaire. Responses requiring additional query were addressed either in-person, via telephone, or video conference. Body size and composition were assessed at Colorado State University using dual energy X-ray absorptiometry (DEXA; Hologic, DiscoveryW, QDR Series, Bedford, MA, USA), and a physician’s digital scale and stadiometer.
The remaining data collection sessions were completed off-campus on six mornings, each separated by 1–2 weeks depending on participant schedules. The time of protocol initiation was kept constant for each participant. Every data collection was preceded by a 12-h fast, 24-h abstention from alcohol and exercise, and 96-h abstention from any products derived from Cannabis sativa L., including CBD and marijuana.
A venous catheter was introduced to an antecubital or dorsal hand vein and blood (~10 mL) was collected for analysis of baseline circulating concentrations of THC, THC-COOH, THC-OH, glucose, and insulin. Immediately following baseline blood collection, participants self-administered one of five edible marijuana products or a marijuana-free control product (described in detail in a subsequent section). Thirty minutes following marijuana (or marijuana-free control) ingestion, participants imbibed a beverage consisting of 75 g of glucose dissolved in 250 mL of water (i.e., an oral glucose tolerance test (OGTT)).
Relative to marijuana ingestion (Time 0), venous blood was sampled for subsequent analysis of circulating concentrations of THC, THC-COOH, and THC-OH at minutes 10, 20, 30, 45, 60, 75, 90, 120, 180, and 240. Blood was immediately transferred into chilled tubes coated with ethylenediaminetetraacetic acid (K3 EDTA) and placed on ice for up to 30 min before isolation of plasma via chilled (4 °C) centrifugation. One milliliter aliquots of plasma were then placed on ice while being transported to the research facility for storage at −80 °C prior to subsequent analysis.
Relative to marijuana ingestion (Time 0), the carbohydrate beverage was imbibed at minute 30. Venous blood was sampled for subsequent analysis of circulating concentrations of glucose at minutes 25 (i.e., post-marijuana but pre-glucose), 35, 40, 45, 50, 60, 75, 90, 105, 120, 135, 150, 180, and 240, and at minutes 25, 45, 75, 105, 135, and 150 for subsequent analysis of plasma insulin concentration. Blood intended for glucose analysis was transferred to chilled tubes containing sodium fluoride (potassium oxalate), and then immediately placed on ice for transport to the research facility where it was evaluated, in duplicate, without delay using an automated analyzer (YSI 2900 STAT Glucose Lactate Analyzer, YSI Inc., Yellow Springs, OH, USA). Blood intended for insulin analysis was processed in an identical manner to the blood used for THC, THC-COOH, and THC-OH analysis. Plasma insulin concentration was determined in triplicate via enzyme-linked immunosorbent assay ((ELISA) Crystal Chem, Inc., Elk Grove Village, IL, USA).
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Publication 2021
Beverages BLOOD Body Size Cannabis sativa Carbohydrates Catheters Centrifugation Dual-Energy X-Ray Absorptiometry Edetic Acid Enzyme-Linked Immunosorbent Assay Ethanol Glucose Insulin isolation Lactates Natural Springs Oral Glucose Tolerance Test Physicians Plasma Potassium Oxalate Sodium Fluoride Veins
Membrane vesicles were resuspended in 1 mL buffer (30 mmol/L Tris-HCl, pH 7.0; 100 mmol/L KCl; 5 mmol/L NaN3; 5 mmol/L MgCl2; 0.15 mmol/L EGTA; 0.12 mmol/L CaCl2; 1 μCi 45Ca2+; and 10 mmol/L potassium oxalate) and were divided into 2 × 500-μL aliquots. One aliquot was incubated with a SERCA2a inhibitor cocktail (10 μmol/L thapsigargin, 100 μmol/L ammonium molybdate, and 0.05 μg bafilomycin) and the other remained untreated. Ca2+ uptake assays were conducted as described by Andersen (30 (link),31 (link)) using Na2ATP to initiate uptake. In assays involving rat heart membrane vesicles, 50 μmol/L ryanodine was added to the incubation media for 10 min to inhibit or close the ryanodine receptor Ca2+ release channel.
Publication 2011
ammonium molybdate Biological Assay Buffers Cardiac Arrest Egtazic Acid Heart Magnesium Chloride Potassium Oxalate Ryanodine Ryanodine Receptors Sodium Azide Thapsigargin Tissue, Membrane Tromethamine
The largemouth bass was obtained from the Sanshui platinum Aquafarm (Foshan, Guangdong, China). All fish were acclimated and fed the control experimental diet (B0) for 2 weeks before the trial. Fish (6.17 ± 0.03 g) were selected and distributed into 36 tanks (256 L) after 24 h starvation with 30 fish per tank and six tanks per treatment. Fish were fed to apparent satiation twice daily at 08:00 and 16:00 for 70 days. The water temperature was maintained at 22–24°C, pH = 7–8, dissolved oxygen (DO) > 6.0 mg/L and NH4-N < 0.5 mg/L. Aeration was supplied to each tank 24 h per day. The photoperiod was 12D: 12L and the light intensity was 400 lx. During the feeding period, recording the feed consumption.
At the end of the feeding trial, fish fasted for 24 h. Then, the fish in each tank were counted and batch weighed to calculate survival rate (SR), final mean body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), voluntary feed intake (VFI), and feed conversion ratio (FCR). All the sampled fish were anesthetized with chlorobutanol (300 mg/L, SINOPHARM, China) before sampling. Individual body weight, viscera, and liver weight of three fish in each tank were recorded to calculate viscerosomatic index (VSI) and hepatosomatic index (HSI). Blood samples were drawn from the caudal part of the sedated fish using anticoagulant syringes with 2% sodium fluoride (SINOPHARM, China) and 4% potassium oxalate (SINOPHARM, China). Blood samples were centrifuged at 1500 g for 10 min at 4°C to obtain plasma, and then stored at −80°C for analysis of hematological parameters. Two liver samples near to the bile duct in each tank were put into 1.5 mL RNase-free tubes (Axygen, United States), fast frozen in liquid nitrogen and then stored at −80°C for mRNA level and western blot analysis, or fixed in 4% paraformaldehyde no longer than 48 h for histology determination. Two liver samples in each tank were put into 1.5 mL tubes, fast frozen in liquid nitrogen and stored at −80°C for detection of hepatic cAMP. Livers from another ten fish in each tank were pooled into ziplock bags and then stored at −20°C for the assay of moisture and crude lipid. Whole bodies of four fish per tank were collected into plastic bags and then stored at −20°C for the assay of moisture, crude protein and crude lipid to calculate productive lipid value (PLV) and productive protein value (PPV).
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Publication 2019
angiogenin Anticoagulants Bass Bile Biological Assay BLOOD Chlorobutanol Diet Duct, Bile Feed Intake Fishes Freezing Hepatic Duct Human Body Light Lipids Liver Nitrogen Oxygen paraform Plasma Platinum Potassium Oxalate Proteins RNA, Messenger Satiation Sodium Fluoride Syringes Viscera Western Blot

Most recents protocols related to «Potassium Oxalate»

Analysing biological samples enables us to objectively evaluate biomarkers that act as an indicator of a person’s health. Biomarkers can also provide an early indication of disease before symptoms arise, provide us with information on disease progression and/or suggest therapies. In NICOLA, non-fasting venous blood samples were obtained from consenting participants. These included blood serum, plasma (EDTA/clot activator), glucose (potassium oxalate/sodium fluoride) and RNA (PAXgene). A spot urine sample was also obtained from all participants. All biological samples were transported in temperature controlled containers to a central laboratory and processed within 4 h. Aliquoted samples were subsequently frozen at − 80 °C until analysis. A dedicated courier service was used for transporting samples collected at the home-based assessments. As described previously, detailed laboratory analysis was conducted on all of the samples which included multi-omic biomarkers, lipid profiling, dietary biomarkers, inflammatory biomarkers and hormones [3 ]. All laboratory assays were standardised against available international standards, and quality control samples were included in every run. Participants consented separately for the collection of blood, DNA, urine, retinal images, facial photograph and the administration of the eye drops including consent for analysis, storage and future contact. Data are currently available for 28 biochemical biomarkers from 3082 participants within the NICOLA cohort. Participants were also offered rapid testing and feedback from blood glucose and lipid levels. NICOLA has a strong focus on molecular biomarkers and complementary genetic, epigenetic and transcriptomic data is available for a subset of participants. There is also 551,830 directly genotyped and 18,148,478 imputed SNPs currently available for 2969 participants.
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Publication 2023
Administration, Ophthalmic Biological Assay Biological Markers Biopharmaceuticals BLOOD Blood Glucose Clotrimazole Diet Disease Progression Edetic Acid Epigenesis, Genetic Face Freezing Gene Expression Profiling Glucose Hormones Inflammation Lipids Plasma Potassium Oxalate Retina Serum Single Nucleotide Polymorphism Sodium Fluoride Urine Veins
SR calcium uptake was measured using a previously described Millipore filtration technique.20 Approximately 150 μg of ventricular protein extract was incubated at 37 °C in 1.5 mL of calcium uptake medium (40 mmol/L imidazole, pH 7.0, 100 mmol/L KCl, 5 mmol/L MgCl2, 5 mmol/L NaN3, 5 mmol/L potassium oxalate, 0.5 mmol/L EGTA), and various concentrations of CaCl2 to yield 0.03–3 μmol/L free calcium. Ruthenium red was added to a final concentration of 1 μmole immediately before adding substrates to begin calcium uptake. A final concentration of 5 mmole ATP was added to initiate the reaction. The reaction was terminated at 1 minute by filtration. The rate of SR calcium uptake and the calcium concentration required for EC50 were determined by nonlinear curve fitting analysis using GraphPad Prism v6.01 software (GraphPad, San Diego, CA).
Publication 2023
Calcium, Dietary Egtazic Acid Filtration Heart Ventricle imidazole Magnesium Chloride Potassium Oxalate prisma Sodium Azide Staphylococcal Protein A
The experimental procedure is outlined in Figure 1.
The fabrication of the bovine deciduous anterior teeth was as follows: epoxy resin (Epofix, Struers, Tokyo, Japan) was poured into bovine-extracted mandibular premolars (n = 5) pulp cavity and stored at 60 °C for 24 h to cure the epoxy resin. Each tooth was longitudinally divided into a four-specimen block using a low-speed rotary cutting machine (IsoMet, Buehler, Lake Bluff, IL, USA). Furthermore, to expose the fresh dentin, they were polished using a manual polisher (EcoMet3, Buehler, Tokyo, Japan) and water-resistant polishing paper (#400–800, Buehler). The specimens were cut perpendicular to the tooth axis into 300 μm-thick sections using a slow rotary cutter. Finally, to prepare single-section specimens, all the surfaces except the exposed dentin surface were coated with a sticky wax. Potassium oxalate monohydrate (Nacalai Tesque, Kyoto, Japan), polyacrylic acid 25,000 (FUJIFILM Wako Pure Chemicals, Osaka, Japan), and malonic acid (FUJIFILM Wako Pure Chemicals, Osaka, Japan), each in 5% solution, were used as the material groups (oxalic acid (OA), polyacrylic acid (PA), succinic acid (SA), and malonic acid (MA) (Figure 2)); deionized distilled water was used as a control (CO).
The bovine tooth single-section samples were immersed in 500 µL of each solution (CO, MA, PA, SA, and OA) for 5 min and then washed three times using 500 µL of deionized distilled water. After the treatment, the groups washed using 500 µL of remineralization solution were designated as (CO_RE, MA_RE, PA_RE, SA_RE, and OA_RE).
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Publication 2023
Bicuspid Bos taurus carbomer 940 Deciduous Tooth Dental Pulp Cavity Dentin Epistropheus Epoxy Resins malonic acid Mandible Oxalic Acid Potassium Oxalate Succinic Acid Tooth
This was a randomized, open-label, two-period, fixed-sequence crossover study (Figure S1). Based on the exploratory and descriptive characteristics of the study aim, the calculation of study power was not considered to determine the sample size. The enrolled subjects were randomly assigned to the aspirin group or the fexuprazan group at a ratio of 1:1. For the aspirin group, a single oral dose of aspirin 500 mg was administered alone in the first period and then in combination with fexuprazan 80 mg after 4 days of pretreatment with once-daily doses of fexuprazan 80 mg in the second period, and there was a 10-day washout period between each period. For the fexuprazan group, once-daily doses of fexuprazan 80 mg were administered alone for 5 days in the first period and then in combination with aspirin 500 mg for 5 days in the second period. The subjects received their respective treatment with 150 mL of water after overnight fasting.
For the aspirin group, serial blood samples were collected at predose, 1.5, 4, 6, 8, 10, 12, 24, and 48 h after aspirin dosing for the PD analysis and at predose, 0.17, 0.33, 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12, 24, 36, and 48 h after aspirin dosing for the PK analysis. For the PD evaluation of aspirin, 16.2 mL of blood was taken in a citrate tube and then analyzed by both arachidonic acid-induced and collagen-induced platelet aggregation assays. For the PK evaluation of aspirin and its metabolite, salicylic acid, 10 mL of blood was taken in a sodium fluoride and potassium oxalate tube at each sampling point and subsequently centrifuged at 3000 rpm for 10 min at 4 °C, and 1.0 mL of supernatant was transferred to Eppendorf tubes and stored at −70 °C until analysis. For the fexuprazan group, serial blood samples for PK analysis were collected at predose, 0.5, 1, 1.5, 2, 2.5, 3, 4, 6, 8, 12, 24, 36, and 48 h after the 5th dose of fexuprazan (i.e., the last dose) and at predose before the 3rd and 4th doses of fexuprazan. For PK evaluation of fexuprazan and its metabolite, M14, 6 mL of blood was taken in a sodium heparin tube for each sampling point and subsequently centrifuged at 3000 rpm for 10 min at 4 °C, and 1.0 mL of supernatant was transferred to Eppendorf tubes and stored at −70 °C until analysis.
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Publication 2023
Arachidonic Acid Aspirin Biological Assay BLOOD Citrates Collagen fexuprazan Hematologic Tests Heparin Sodium Platelet Aggregation Potassium Oxalate Salicylic Acid Sodium Fluoride
Blood was sampled at the end of periods 1 and 2 in the morning when measures noted above were made, and samples were also taken after period 3. Samples were collected by jugular venipuncture into two tubes used for plasma, one with sodium fluoride and potassium oxalate and another with sodium heparin, and a third tube without an anticoagulant for serum. Plasma and serum were harvested by centrifugation for 20 min at 3000× g and frozen at −20 °C. Plasma from the sodium fluoride and potassium oxalate tube was analyzed for glucose and lactate, respectively, with a USI 2300 Plus Glucose & Lactate Analyzer (YSI Inc., Yellow Springs, OH, USA). Plasma from the sodium heparin tubes and (or) serum was analyzed for constituents such as non-esterified fatty acids (NEFA), triglycerides, cholesterol, urea, albumin, and total protein with a Vet Axcel® Chemistry Analyzer (Alfa Wassermann Diagnostic Technologies, West Caldwell, NJ, USA) according to the manufacturer’s instructions. Plasma was analyzed for total antioxidant capacity (TAC) colorimetrically with a Technicon Autoanalyzer II System (Technicon Instruments, Tarrytown, NY, USA) based on a ferric reducing ability of plasma [29 (link)].
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Publication 2023
Albumins Anticoagulants Antioxidants BLOOD Centrifugation Cholesterol Diagnosis Fatty Acids, Esterified Freezing Glucose Heparin Sodium Lactate Natural Springs Plasma Potassium Oxalate Serum Sodium Fluoride Staphylococcal Protein A Triglycerides Urea Venipuncture

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More about "Potassium Oxalate"

Potassium Oxalate (K2C2O4) is a versatile chemical compound with a wide range of applications in scientific research and industrial processes.
Also known as Oxalate of Potash or Potassium Binoxalate, this salt of oxalic acid and potassium is prized for its unique properties and diverse uses.
In the field of analytical chemistry, Potassium Oxalate is commonly used as a reagent in various assays and procedures.
It finds applications in the Cobas analyzer, a widely used clinical chemistry platform, as well as the EmulsiFlex-C5, a high-pressure homogenizer employed for cell disruption and sample preparation.
Potassium Oxalate also plays a role in the FLIPR Tetra, a fluorescence-based assay system used to study ion channel and receptor activity.
Biochemistry and protein purification researchers often utilize Potassium Oxalate in conjunction with Ni-NTA Superflow resin and HisTrap FF crude columns for the efficient purification of recombinant proteins tagged with histidine.
Additionally, Potassium Oxalate is employed in the BD Vacutainer system, a widely used collection and storage device for blood and plasma samples.
In the field of molecular biology, Potassium Oxalate has been utilized in the XK16 column, a chromatographic tool for the purification of biomolecules, as well as the Storm phosphorimager, a device used for the detection and quantification of radioactively labeled nucleic acids and proteins.
The Vacutainer plasma collection tube, designed for the collection and storage of plasma samples, may also contain Potassium Oxalate as an anticoagulant.
Furthermore, the AU480 clinical chemistry analyzer, a widely used instrument for the measurement of various analytes in biological samples, may employ Potassium Oxalate in its assay protocols.
Overall, Potassium Oxalate's versatility and importance in scientific research and industrial applications make it a compound of great interest to researchers and professionals across various disciplines.