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> Chemicals & Drugs > Organic Chemical > Propidium Iodide

Propidium Iodide

Propidium iodide is a fluorescent dye that binds to DNA and is commonly used in flow cytometry and microscopy to detect cell death and assess cell viability.
It is membrane-impermeant and can only enter cells with compromised membranes, making it a useful marker for late apoptotic and necrotic cells.
Propidium iodide has applications in research, clinical diagnostics, and drug discovery, enabling the quantification of cell death and the evaluation of cell health.
PubCompare.ai's AI-driven optimized protocols can help you locate the most reproducible and accurate propidium iodide research from literature, preprints, and patents, allowing you to identify the best products and protocols for your needs.
Trust PubCompare.ai for seamless, data-driven research to advance your studies.

Most cited protocols related to «Propidium Iodide»

1
Genome-wide Analysis of Somatic Copy Number Alterations in Cancer
DNA extracted from cancer specimens and normal tissue was labeled and hybridized to the Affymetrix 250K Sty I array to obtain signal intensities and genotype calls. Signal intensities were normalized against data from 1480 normal samples. Copy-number profiles were inferred using GLAD48 (link) and changes of > 0.1 copies in either direction were called SCNAs. The significance of focal SCNAs (covering < 0.5 chromosome arms) was determined using GISTIC18 (link), with modifications to score SCNAs directly proportional to amplitude and to allow summation of non-overlapping deletions affecting the same gene. Peak region boundaries were determined so that the change in the GISTIC score from peak to boundary had < 5% likelihood of occurring by random fluctuation. P-values for Figures 2b and 4 were determined by comparing the gene densities of SCNAs and fraction overlap of peak regions respectively to the same quantities calculated from random permutations of the locations of these SCNAs and peak regions. RNAi was performed by inducible and stable expression of shRNA lentiviral vectors and by siRNA transfection. Proliferation in inducible shRNA experiments was measured in triplicate every half-hour on 96-well plates by a real time electric sensing system (ACEA Bioscience) and in stable shRNA expression and siRNA transfection experiments by CellTiterGlo (Promega). Apoptosis was measured by immunoblot against cleaved PARP and FACS analysis of cells stained with antibody to annexin V and propidium iodide. Tumor growth in nude mice was measured by caliper twice weekly. Expression of MYC, MCL1, and BCL2L1 was performed with retroviral vectors in lung epithelial cells immortalized by introduction of SV40 and hTERT49 (link).
Full methods are described in Supplementary Methods.
Beroukhim R., Mermel C.H., Porter D., Wei G., Raychaudhuri S., Donovan J., Barretina J., Boehm J.S., Dobson J., Urashima M., Mc Henry K.T., Pinchback R.M., Ligon A.H., Cho Y.J., Haery L., Greulich H., Reich M., Winckler W., Lawrence M.S., Weir B.A., Tanaka K.E., Chiang D.Y., Bass A.J., Loo A., Hoffman C., Prensner J., Liefeld T., Gao Q., Yecies D., Signoretti S., Maher E., Kaye F.J., Sasaki H., Tepper J.E., Fletcher J.A., Tabernero J., Baselga J., Tsao M.S., DeMichelis F., Rubin M.A., Janne P.A., Daly M.J., Nucera C., Levine R.L., Ebert B.L., Gabriel S., Rustgi A.K., Antonescu C.R., Ladanyi M., Letai A., Garraway L.A., Loda M., Beer D.G., True L.D., Okamoto A., Pomeroy S.L., Singer S., Golub T.R., Lander E.S., Getz G., Sellers W.R, & Meyerson M. (2010). The landscape of somatic copy-number alteration across human cancers. Nature, 463(7283), 899-905.
Publication 2010
Annexin A5 Apoptosis Arm, Upper bcl-X Protein Cells Chromosomes Cloning Vectors Electricity Epithelial Cells Gene Deletion Genes Genotype Immunoblotting Immunoglobulins Lung Malignant Neoplasms MCL1 protein, human Mice, Nude Neoplasms Promega Propidium Iodide Retroviridae RNA, Small Interfering RNA Interference Short Hairpin RNA Simian virus 40 Tissues Transfection
2
Genome-wide Analysis of Somatic Copy Number Alterations in Cancer
DNA extracted from cancer specimens and normal tissue was labeled and hybridized to the Affymetrix 250K Sty I array to obtain signal intensities and genotype calls. Signal intensities were normalized against data from 1480 normal samples. Copy-number profiles were inferred using GLAD48 (link) and changes of > 0.1 copies in either direction were called SCNAs. The significance of focal SCNAs (covering < 0.5 chromosome arms) was determined using GISTIC18 (link), with modifications to score SCNAs directly proportional to amplitude and to allow summation of non-overlapping deletions affecting the same gene. Peak region boundaries were determined so that the change in the GISTIC score from peak to boundary had < 5% likelihood of occurring by random fluctuation. P-values for Figures 2b and 4 were determined by comparing the gene densities of SCNAs and fraction overlap of peak regions respectively to the same quantities calculated from random permutations of the locations of these SCNAs and peak regions. RNAi was performed by inducible and stable expression of shRNA lentiviral vectors and by siRNA transfection. Proliferation in inducible shRNA experiments was measured in triplicate every half-hour on 96-well plates by a real time electric sensing system (ACEA Bioscience) and in stable shRNA expression and siRNA transfection experiments by CellTiterGlo (Promega). Apoptosis was measured by immunoblot against cleaved PARP and FACS analysis of cells stained with antibody to annexin V and propidium iodide. Tumor growth in nude mice was measured by caliper twice weekly. Expression of MYC, MCL1, and BCL2L1 was performed with retroviral vectors in lung epithelial cells immortalized by introduction of SV40 and hTERT49 (link).
Full methods are described in Supplementary Methods.
Beroukhim R., Mermel C.H., Porter D., Wei G., Raychaudhuri S., Donovan J., Barretina J., Boehm J.S., Dobson J., Urashima M., Mc Henry K.T., Pinchback R.M., Ligon A.H., Cho Y.J., Haery L., Greulich H., Reich M., Winckler W., Lawrence M.S., Weir B.A., Tanaka K.E., Chiang D.Y., Bass A.J., Loo A., Hoffman C., Prensner J., Liefeld T., Gao Q., Yecies D., Signoretti S., Maher E., Kaye F.J., Sasaki H., Tepper J.E., Fletcher J.A., Tabernero J., Baselga J., Tsao M.S., DeMichelis F., Rubin M.A., Janne P.A., Daly M.J., Nucera C., Levine R.L., Ebert B.L., Gabriel S., Rustgi A.K., Antonescu C.R., Ladanyi M., Letai A., Garraway L.A., Loda M., Beer D.G., True L.D., Okamoto A., Pomeroy S.L., Singer S., Golub T.R., Lander E.S., Getz G., Sellers W.R, & Meyerson M. (2010). The landscape of somatic copy-number alteration across human cancers. Nature, 463(7283), 899-905.
Publication 2010
Annexin A5 Apoptosis Arm, Upper bcl-X Protein Cells Chromosomes Cloning Vectors Electricity Epithelial Cells Gene Deletion Genes Genotype Immunoblotting Immunoglobulins Lung Malignant Neoplasms MCL1 protein, human Mice, Nude Neoplasms Promega Propidium Iodide Retroviridae RNA, Small Interfering RNA Interference Short Hairpin RNA Simian virus 40 Tissues Transfection
3
Automated Comet Assay Image Analysis

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Open the protocol to access the free full text link

Publication 2014
1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidine)quinolinium, tetraiodide Cells Comet Assay DAPI DNA Damage EDNRB protein, human Fluorescent Dyes Gold Head Light Propidium Iodide Silver Nitrate SYBR Green I Tail
4
Isolation and Culture of Intestinal Organoids
Described previously 2 (link), 4 (link). For culture/sorting experiments, at least 3 independent experiments were performed. For each experiment, crypts/cells were pooled from 3 intestines. For microarray, sorted cells from 10 intestines were pooled. Crypts were directly cultured as previously described (100 crypts/well on 24-well plate) 4 (link). For single-cell or doublet-cell culture, crypts were dissociated with TrypLE express (Invitrogen) including 2000 U/ml DNase (Sigma) for 30 min at 37 °C or 2 hr at room temperature. For reassociation assay from established crypt organoids, the samples were dissociated with TrypLE express for 15 min at 37 °C. Dissociated cells were passed through 20- μm cell strainer (Celltrix) and washed with PBS. Cells were stained with PE- conjugated anti-CD24 antibody (eBioscience) and APC- conjugated anti-Epcam antibody (eBioscience) for 15 min at 4 °C, and analyzed by MoFlo (DakoCytomation). Viable epithelial single-cells or doublets were gated by forward scatter, side scatter and pulse-width parameter, and negative staining for propidium iodide or 7-ADD (eBioscience). Sorted cells were collected, pelleted, and embedded in Matrigel (BD bioscience), followed by seeding on 96-well plate (30-50 singlets or doublets/10 μl Matrigel/well. Culture medium (Advanced DMEM/F12 supplemented with Penicillin/Streptomycin, 10 mM Hepes, Glutamax, 1× N2, 1× B27 (all from Invitrogen), and 1 μM N-acetylcysteine (Sigma) containing growth factors: 50 ng/ml EGF, 100 ng/ml noggin, 1 μg/ml R-spondin) were overlaid. Y-27632 (10 μM) was included for the first 2 days to avoid anoikis. Growth factors were added every other day and the entire medium was changed every 4 days. In some experiments, 100 ng/ml Wnt-3a (Millipore) was added in the culture medium. Sorted cells were manually inspected by inverted microscopy, and the numbers of viable organoids in triplicate were calculated.
Sato T., van Es J.H., Snippert H.J., Stange D.E., Vries R.G., van den Born M., Barker N., Shroyer N.F., van de Wetering M, & Clevers H. (2010). Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts. Nature, 469(7330), 415-418.
Publication 2010
Acetylcysteine Anoikis Antibodies, Anti-Idiotypic Biological Assay Cell Culture Techniques Cells CFC1 protein, human Culture Media Deoxyribonucleases Epithelial Cells Growth Factor HEPES Intestines matrigel Microarray Analysis Microscopy noggin protein Organoids Penicillins Propidium Iodide Pulse Rate Streptomycin TACSTD1 protein, human Y 27632
5
Protocols for DNA Damage Response Research
U2OS-based lines were maintained under standard conditions. cDNA cloning was by standard procedures. siRNA transfections were with Lipofectamine RNAiMAX (Invitrogen). IR was administered with a Faxitron X-ray machine (Faxitron X-ray Corporation). ATM inhibition was by KU-55933 (KuDOS Pharmaceuticals). Laser micro-irradiation was with a FluoView 1000 confocal microscope (Olympus) with 37°C heating stage (Ibidi) and 405 nm diode (6 mW). FRAP was performed when laser-track accumulation of GFP-tagged protein reached maximal steady-state level. For immunofluorescence, cells were pre-extracted or not, fixed with 2% paraformaldehyde, permeabilized and stained. For whole cell extracts, cells were lysed on plates with 2% SDS, 50 mM Tris-HCl pH 7.5, 20 mM N-ethylmaleimide (Sigma-Aldrich) and protease inhibitor cocktail (Roche). To immunoprecipitate 53BP1, BRCA1 and sumoylated proteins, different lysis and binding buffers were used (Supplementary Information). HR and NHEJ assays were as previously described17 (link),28 (link). For IR survival, cells were transfected with siRNA and exposed to IR. After 10-14 days, colonies were stained with 0.5% crystal violet/20% ethanol, counted and normalized to plating efficiencies. For Florescence-Activated Cell Sorting (FACS) of propidium iodide-stained cells, data were analyzed by FlowJo software. All error-bars represent STDEV. Detailed descriptions of methods are provided in Supplementary Information.
Galanty Y., Belotserkovskaya R., Coates J., Polo S., Miller K.M, & Jackson S.P. (2009). Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses to DNA double-strand breaks. Nature, 462(7275), 935-939.
Publication 2009
Biological Assay BRCA1 protein, human Buffers Cell Extracts Cells DNA, Complementary Ethanol Ethylmaleimide Immunofluorescence KU 55933 Lipofectamine Microscopy, Confocal Non-Homologous DNA End-Joining paraform Pharmaceutical Preparations Propidium Iodide Protease Inhibitors Proteins Psychological Inhibition Radiography Radiotherapy RNA, Small Interfering TP53BP1 protein, human Transfection Tromethamine Violet, Gentian

Most recents protocols related to «Propidium Iodide»

1
MSLN-BiTE Binding Affinity Assay
Not available on PMC !

Example 10

Binding of MSLN-BiTE to membrane-bound target expressed in cells was determined with an on-cell affinity assay. 3×104 cells per well of a microtiter plate were incubated with MSLN-BiTE protein in a dose response for 16-22 h at 4° C. Cells were washed twice with flow buffer (PBS that contained 2% fetal calf serum and 0.01% sodium azide), and then resuspended in flow buffer and incubated with an anti-His Fab labeled with Alexa Fluor-647 for 50 minutes at 4° C. Cells were fixed after incubation to optimize detection of the fluorescent signal. Cells were then washed twice and resuspended in flow buffer that contained propidium iodide at 1 ug/ml. Cells were analyzed by flow cytometry for live cells that were positive for Alexa Fluor-647. EC50 values were determined from the dose response curve of Alexa Fluor-647 positive cells.

FIG. 20 shows the results of binding of representative MSLN-BiTE proteins to human MSLN in NCI-N87 gastric cancer cells and to human CD3 in HPB-ALL cells. Solid lines in the graphs below indicate VH-VL orientation and dotted lines indicate the VL-VH orientation. FIG. 22 shows the results of binding of representative MSLN-BiTE proteins to human MSLN in OVCAR-8 ovarian cancer cells and to human or cyno MSLN in 293T cells that are transiently transfected with human MSLN or cyno MSLN.

US11866508B2. Anti-mesothelin binding proteins (2024-01-09). AMGEN INC. [US]. Inventors: William Christian Fanslow, III [US], Carl Kozlosky [US], Jean Gudas [US].
Full text: Click here
Patent 2024
Alexa Fluor 647 Binding Proteins Biological Assay Buffers Cells Dental Occlusion Fetal Bovine Serum Flow Cytometry Gastric Cancer HEK293 Cells Homo sapiens MSLN protein, human Ovarian Cancer Propidium Iodide Proteins Signal Detection (Psychology) Sodium Azide Tissue, Membrane
2
Cytotoxicity Assay for AQP4-Expressing Cells
Not available on PMC !

Example 3

After HEK293 cells were transfected with hAQP4-plasmid DNA for 24 hours, the cells were incubated with NMO-IgG or A002, or with normal human serum (1:20) or anti-beta actin mouse mAb with 5% human complement for 90 min at 37° C. After washed by 1X PBS, cells were incubated with 10 nM Calcein-AM for 15 min at 37° C. Then cells were incubated with 10 μM Propidium Iodide for 15 min at 37° C. after washed. After washed, cells were incubated with 4% formaldehyde for 10 min at room temperature. The images were obtained by microscopy and the results were shown in FIG. 8.

US11858989B2. Anti-AQP4 antibodies (2024-01-02). None [TW]. Inventors: Chao-Lin Lee [TW].
Full text: Click here
Patent 2024
beta-Actin Biological Assay Cells Complement 5 fluorexon Formaldehyde HEK293 Cells Homo sapiens Microscopy Mus Plasmids Propidium Iodide Serum
3
Bipartite Engager Binding Assay
Not available on PMC !

Example 16

The binding of bipartite (FAP-CD3) engager molecules to T cells will be assessed. Briefly, 25,000 T cells are stimulated with 200 U/mL IL-2 for 12 days. After 12 days, T cell are incubated with varying concentrations of engager molecules (500, 1000, or 2000 ng/mL) for 20 minutes at room temperature in triplicate. Cells are then washed twice, followed by staining with an anti-6×His APC antibody at 500 ng/mL for an additional 20 minutes. Cells are washed again and treated with propidium iodide (PI) to exclude dead cells from further analysis. Stained cells are analyzed by flow cytometry on a BD LSR Fortesa cytometer and the percentage of the cell population positive for staining was set at 2% of the secondary only control.

Assessment of binding of bipartite (FAP-CD3) engager molecules to tumor cells. Briefly, HEK293 stably overexpressing FAB or negative control cells (HEK293) are treated with 500 or 1000 ng/mL of either (i) His-tagged soluble FAP or (ii) His-tagged Fc negative control protein. Cells are stained with a fluorescently labelled anti-His antibody and analyze by flow cytometry.

US11865081B2. Oncolytic viral delivery of therapeutic polypeptides (2024-01-09). Virogin Biotech Canada Ltd. [CA]. Inventors: Mitchell H. Finer [US], Luke Evnin [US].
Full text: Click here
Patent 2024
CD3 Antigens Cells Flow Cytometry Immunoglobulins Neoplasms Propidium Iodide Proteins T-Lymphocyte
4
Cytotoxicity Assay of MSLN-Targeting BiTE Molecules
Not available on PMC !

Example 5

Throughout the specification, and in particular the examples, the terms “2X” and “3X” in reference to the anti-MSLN antibodies are used. These terms refer to substitutions in the Fc region that result in enhanced ADCC through increased binding affinity to the Fc receptor. The “2X” molecule has the following substitutions: S239D/I332E and the “3X” molecule has the following mutations: S239D/I332E/A330L.

Example 12

Cytoxicity assays for the MSLN-BiTE molecules were performed generally as described above in Example 5, except that pan-T cells were used as effector cells. BiTE proteins were incubated with MSLN-expressing tumor cells OVCAR-8 at 2500 cells per well of a microtiter plate, and unstimulated pan-T cells (source: ALL CELLS) in a 10:1 effector to target cells ratio, for 48 h at 37° C., 5% CO2. Cells were then stained with 10 uM Hoechst 33342 to visualize the cell nuclei, and 2 uM propidium iodide to specifically label the nuclei of dead cells. After incubation with the DNA stains for 90 min at 37° C., 5% CO2, the microplate wells were scanned by high content imaging (ArrayScan) to capture images of the cells. Percent specific cytotoxicity was calculated using the formula: [1−live target cell count (+BiTE)/live target cell count (−BiTE)]×100. Results are summarized in FIGS. 25 and 26.

US11866508B2. Anti-mesothelin binding proteins (2024-01-09). AMGEN INC. [US]. Inventors: William Christian Fanslow, III [US], Carl Kozlosky [US], Jean Gudas [US].
Full text: Click here
Patent 2024
Adrenocortical Carcinoma, Hereditary Anti-Antibodies Binding Proteins Biological Assay Cell Nucleus Cells Cytotoxin Dental Occlusion Fc Receptor Figs HOE 33342 Mesothelin MSLN protein, human Mutation Neoplasms Propidium Iodide Proteins Staining T-Lymphocyte
5
Antibody Cytotoxicity Assay in AQP4-GFP Cells
Not available on PMC !

Example 4

HEK293-hAQP4-GFP and LPS-stimulated RAW264.7 were co-cultured with CD4 antibody, commercial AQP4 antibody, A002 antibody (ten-fold serial dilution) or culture medium only at 37° C. and 5% CO 2 for 6 hours. Cells were stain with Fixable Far Red-labeled anti-amine, PE-labeled anti-mouse CD11 b then analyzed the % of amine in CD11b-/GFP+ cells. Cell death(%) increased in ADCC=(% cell death in presence of IgG-% cell death in absence of IgG)/(% Cell death in maximum lysis-% cell death in absence of IgG)×100. Antibody-dependent cell-mediated cytotoxicity Assay (ADCC) of A002 antibody by immunofluorescent stain. HEK293-hAQP4-GFP and LPS-stimulated RAW264.7 were co-cultured with CD4 antibody, commercial AQP4 antibody, A002 antibody (ten-fold serial dilution) or culture medium only at 37° C. and 5% CO2 for 6 hours. Then cells were stain with Propidium Iodide (PI). Histograms show quantification of Propidium Iodide (PI) of cells co-cultured with CD4 antibody, commercial AQP4 antibody or A002 antibody (ten-fold serial dilution) (FIG. 9). PI intensity was adjusted by Subtracting PI intensity of cells co-cultured with culture medium only.

US11858989B2. Anti-AQP4 antibodies (2024-01-02). None [TW]. Inventors: Chao-Lin Lee [TW].
Full text: Click here
Patent 2024
Amines Biological Assay Cell Death Cells Cytotoxicities, Antibody-Dependent Cell Fluorescent Antibody Technique Immunoglobulins ITGAM protein, human Mus Propidium Iodide Stains Technique, Dilution

Top products related to «Propidium Iodide»

1
Propidium iodide by Merck Group
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Propidium iodide is a fluorescent dye commonly used in molecular biology and flow cytometry applications. It binds to DNA and is used to stain cell nuclei, allowing for the identification and quantification of cells in various stages of the cell cycle.
2
Facscalibur by BD
Sourced in United States, Germany, United Kingdom, China, Canada, Japan, Italy, France, Belgium, Switzerland, Singapore, Uruguay, Australia, Spain, Poland, India, Austria, Denmark, Netherlands, Jersey, Finland, Sweden
The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.
3
Facscalibur flow cytometer by BD
Sourced in United States, Germany, United Kingdom, China, Canada, Japan, Belgium, France, Spain, Italy, Australia, Finland, Poland, Switzerland, Cameroon, Uruguay, Denmark, Jersey, Moldova, Republic of, Singapore, India, Brazil
The FACSCalibur flow cytometer is a compact and versatile instrument designed for multiparameter analysis of cells and particles. It employs laser-based technology to rapidly measure and analyze the physical and fluorescent characteristics of cells or other particles as they flow in a fluid stream. The FACSCalibur can detect and quantify a wide range of cellular properties, making it a valuable tool for various applications in biology, immunology, and clinical research.
4
Propidium iodide by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, France, Australia, Canada, Netherlands, Japan, Spain, Italy, Belgium, India, Portugal, Austria, Poland, Switzerland, Sweden, Malaysia, Brazil, Hong Kong, Ireland, Denmark, Singapore, New Zealand
Propidium iodide is a fluorescent dye used in flow cytometry to stain and quantify DNA content in cells. It binds to DNA by intercalating between the bases. Propidium iodide is commonly used to distinguish viable from non-viable cells.
5
Cellquest software by BD
Sourced in United States, Germany, United Kingdom, Italy, Canada, China, Japan, Belgium, France, Spain, Switzerland, Poland, Uruguay, Denmark, Singapore
CellQuest software is a data acquisition and analysis software designed for flow cytometry applications. It provides tools for acquiring, processing, and analyzing flow cytometry data.
6
Propidium iodide by BD
Sourced in United States, Germany, Belgium, China, United Kingdom, Canada, France, Italy, India, Poland
Propidium iodide is a fluorescent dye used in various laboratory applications, particularly in cell biology and flow cytometry. It is a nucleic acid intercalator that binds to DNA, emitting a red fluorescence upon excitation. Propidium iodide is commonly used to stain and identify dead or dying cells in a sample.
7
Rnase a by Merck Group
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RNase A is a ribonuclease enzyme used in molecular biology laboratories. It functions by catalyzing the hydrolysis of single-stranded RNA, cleaving the phosphodiester bonds between nucleotides.
8
Facscanto 2 by BD
Sourced in United States, Germany, United Kingdom, Belgium, China, Australia, France, Japan, Italy, Spain, Switzerland, Canada, Uruguay, Netherlands, Czechia, Jersey, Brazil, Denmark, Singapore, Austria, India, Panama
The FACSCanto II is a flow cytometer instrument designed for multi-parameter analysis of single cells. It features a solid-state diode laser and up to four fluorescence detectors for simultaneous measurement of multiple cellular parameters.
9
Facscan by BD
Sourced in United States, Germany, China, United Kingdom, Italy, Japan, Spain, France, Canada, Switzerland, Jersey, Macao
The FACScan is a flow cytometry instrument manufactured by BD. It is designed to analyze and sort cells or particles in a fluid stream. The FACScan uses laser technology to detect and measure the physical and fluorescent characteristics of individual cells or particles as they pass through the instrument's flow cell.
10
Fitc annexin 5 apoptosis detection kit by BD
Sourced in United States, Germany, China, United Kingdom, Poland, Belgium, Japan, Canada, India, Australia
The FITC Annexin V Apoptosis Detection Kit is a laboratory reagent used to detect and quantify apoptosis, a form of programmed cell death, in cell samples. The kit contains FITC-conjugated Annexin V, a protein that binds to phosphatidylserine, a molecule that is externalized during the early stages of apoptosis. The kit also includes a propidium iodide solution, which can be used to identify late-stage apoptotic or necrotic cells.

More about "Propidium Iodide"

Propidium iodide (PI) is a versatile fluorescent dye commonly used in flow cytometry, microscopy, and various research applications.
As a DNA-binding agent, PI is a crucial tool for detecting cell death and assessing cell viability.
Its membrane-impermeant property ensures that it only enters cells with compromised membranes, making it an effective marker for late apoptotic and necrotic cells.
PI has a wide range of applications in research, clinical diagnostics, and drug discovery.
It enables the quantification of cell death and the evaluation of cell health, supporting advancements in fields such as cell biology, immunology, and cancer research.
PubCompare.ai's AI-driven optimized protocols can help researchers locate the most reproducible and accurate PI-related studies from literature, preprints, and patents.
This allows users to identify the best products and protocols for their specific needs, streamlining their research process and ensuring reliable results.
In addition to PI, related tools like the FACSCalibur flow cytometer, CellQuest software, RNase A, FACSCanto II, FACScan, and FITC Annexin V Apoptosis Detection Kit can also be leveraged to complement PI-based investigations.
These integrated solutions provide a comprehensive toolkit for researchers to explore cell health, death, and apoptosis with precision and confidence.
By utilizing PubCompare.ai's AI-powered insights and optimized protocols, researchers can trust in the seamless, data-driven approach to advance their studies on Propidium iodide and related topics.